//proteindomain/activation
type: Collection
persistent identity: proteindomain activation
display id: proteindomain_activation
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: VP64
member: BBa_K801043
member: BBa_K352001
member: BBa_K801042
member: BBa_K1333200
member: BBa_K1333203
member: BBa_K1333201
member: BBa_K1333202
member: BBa_K801039

BBa_K801039
type: ComponentDefinition
persistent identity: BBa K801039
display id: BBa_K801039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-GAL4AD-linker-PIF3 (part for GAL4/LexA based light-switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CDS for a synthetic hybrid fusion protein. Consists of serveral domains. RFC10 pre and suffix
mutable notes: -
mutable provenance: CDS for a synthetic hybrid fusion protein
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GAL4 activation domain (GAL4AD)
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: Linker
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: EcoRI/MfeI fusion scar
sequence annotation: HA epitope tag
sequence annotation: T7 promoter (for sequencing)
sequence: https://synbiohub.org/public/igem/BBa_K801039_sequence/1

GAL4 activation domain (GAL4AD)
type: SequenceAnnotation
persistent identity: annotation2199997
display id: annotation2199997
version: 1
top level: BBa_K801039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199997/range2199997/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199997/range2199997/1
type: Range
persistent identity: range2199997
display id: range2199997
version: 1
direction: direction/forward
top level: BBa_K801039
start: 70
end: 408
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2199998
display id: annotation2199998
version: 1
top level: BBa_K801039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199998/range2199998/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199998/range2199998/1
type: Range
persistent identity: range2199998
display id: range2199998
version: 1
direction: direction/forward
top level: BBa_K801039
start: 503
end: 801
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2200008
display id: annotation2200008
version: 1
top level: BBa_K801039
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200008/range2200008/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200008/range2200008/1
type: Range
persistent identity: range2200008
display id: range2200008
version: 1
direction: direction/forward
top level: BBa_K801039
start: 409
end: 498
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199929
display id: annotation2199929
version: 1
top level: BBa_K801039
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199929/range2199929/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199929/range2199929/1
type: Range
persistent identity: range2199929
display id: range2199929
version: 1
direction: direction/forward
top level: BBa_K801039
start: 31
end: 51
orientation: inline

EcoRI/MfeI fusion scar
type: SequenceAnnotation
persistent identity: annotation2200000
display id: annotation2200000
version: 1
top level: BBa_K801039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200000/range2200000/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200000/range2200000/1
type: Range
persistent identity: range2200000
display id: range2200000
version: 1
direction: direction/forward
top level: BBa_K801039
start: 499
end: 504
orientation: inline

HA epitope tag
type: SequenceAnnotation
persistent identity: annotation2200012
display id: annotation2200012
version: 1
top level: BBa_K801039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200012/range2200012/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200012/range2200012/1
type: Range
persistent identity: range2200012
display id: range2200012
version: 1
direction: direction/forward
top level: BBa_K801039
start: 457
end: 477
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2200010
display id: annotation2200010
version: 1
top level: BBa_K801039
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200010/range2200010/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200010/range2200010/1
type: Range
persistent identity: range2200010
display id: range2200010
version: 1
direction: direction/forward
top level: BBa_K801039
start: 414
end: 436
orientation: inline

VP64
type: ComponentDefinition
persistent identity: BBa J176013
display id: BBa_J176013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VP64
creator: Karmella Haynes
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tetrameric VP16 transc. activator domain
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: VP16
sequence: https://synbiohub.org/public/igem/BBa_J176013_sequence/1

VP16
type: SequenceAnnotation
persistent identity: annotation2166132
display id: annotation2166132
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166132/range2166132/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166132/range2166132/1
type: Range
persistent identity: range2166132
display id: range2166132
version: 1
direction: direction/forward
top level: VP64
start: 118
end: 150
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2166131
display id: annotation2166131
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166131/range2166131/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166131/range2166131/1
type: Range
persistent identity: range2166131
display id: range2166131
version: 1
direction: direction/forward
top level: VP64
start: 79
end: 111
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2166129
display id: annotation2166129
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166129/range2166129/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166129/range2166129/1
type: Range
persistent identity: range2166129
display id: range2166129
version: 1
direction: direction/forward
top level: VP64
start: 1
end: 33
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2166130
display id: annotation2166130
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166130/range2166130/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166130/range2166130/1
type: Range
persistent identity: range2166130
display id: range2166130
version: 1
direction: direction/forward
top level: VP64
start: 40
end: 72
orientation: inline

BBa_K1333202
type: ComponentDefinition
persistent identity: BBa K1333202
display id: BBa_K1333202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda cI-LGF2(R74A)
creator: Yanwen Xie
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fuse lambda cI with LGF2 mutated from Arg to Ala at site 74 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes a stronger interaction with GAL11P compared to the normalized WT.
mutable notes: The LGF2 is mutated from CGA to GCA at site 74.
mutable provenance: It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TGA stop codon
sequence annotation: lambda cI
sequence annotation: ATG start codon
sequence annotation: LGF2(R74A)
sequence: https://synbiohub.org/public/igem/BBa_K1333202_sequence/1

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2385729
display id: annotation2385729
version: 1
top level: BBa_K1333202
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1333202/annotation2385729/range2385729/1

https://synbiohub.org/public/igem/BBa_K1333202/annotation2385729/range2385729/1
type: Range
persistent identity: range2385729
display id: range2385729
version: 1
direction: direction/forward
top level: BBa_K1333202
start: 841
end: 843
orientation: inline

lambda cI
type: SequenceAnnotation
persistent identity: annotation2385730
display id: annotation2385730
version: 1
top level: BBa_K1333202
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333202/annotation2385730/range2385730/1

https://synbiohub.org/public/igem/BBa_K1333202/annotation2385730/range2385730/1
type: Range
persistent identity: range2385730
display id: range2385730
version: 1
direction: direction/forward
top level: BBa_K1333202
start: 1
end: 711
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2385728
display id: annotation2385728
version: 1
top level: BBa_K1333202
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1333202/annotation2385728/range2385728/1

https://synbiohub.org/public/igem/BBa_K1333202/annotation2385728/range2385728/1
type: Range
persistent identity: range2385728
display id: range2385728
version: 1
direction: direction/forward
top level: BBa_K1333202
start: 1
end: 3
orientation: inline

LGF2(R74A)
type: SequenceAnnotation
persistent identity: annotation2385731
display id: annotation2385731
version: 1
top level: BBa_K1333202
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333202/annotation2385731/range2385731/1

https://synbiohub.org/public/igem/BBa_K1333202/annotation2385731/range2385731/1
type: Range
persistent identity: range2385731
display id: range2385731
version: 1
direction: direction/forward
top level: BBa_K1333202
start: 721
end: 840
orientation: inline

BBa_K801043
type: ComponentDefinition
persistent identity: BBa K801043
display id: BBa_K801043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA based yeast light-swithable promoter system
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: composite part of Bba_K319003, K801039, BBa_K801011 , Bba_K319003, Bba_K801041, and BBa_K801011
mutable notes: -
mutable provenance: composite part of Bba_K319003, K801039, BBa_K801011 , Bba_K319003, Bba_K801041, and BBa_K801011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Linker
sequence annotation: BBa_K319003 (pTEF1, TEF1 promoter)
sequence annotation: BBa_K801041 (SV40NLS-PhyB-Linker-LexA)
sequence annotation: BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
sequence annotation: SV40 NLS (SV40 nuclear localization signal)
sequence annotation: BBa_K801011, (tTEF1, TEF1 terminator)
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: LexA
sequence annotation: BBa_K365005 (20 amino acids long linker)
sequence annotation: GAL4AD (GAL4 transcriptonal activation domain)
sequence annotation: T7 promoter (for sequencing)
sequence annotation: Phytochrome B (PhyB, 908 first N-terminal amino acids)
sequence annotation: HA epitope tage
sequence annotation: SV40NLS-PhyB-Linker-LexA
sequence: https://synbiohub.org/public/igem/BBa_K801043_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2198801
display id: annotation2198801
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198801/range2198801/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198801/range2198801/1
type: Range
persistent identity: range2198801
display id: range2198801
version: 1
direction: direction/forward
top level: BBa_K801043
start: 864
end: 959
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198806
display id: annotation2198806
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198806/range2198806/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198806/range2198806/1
type: Range
persistent identity: range2198806
display id: range2198806
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1782
end: 2231
orientation: inline

BBa_K801041 (SV40NLS-PhyB-Linker-LexA)
type: SequenceAnnotation
persistent identity: annotation2198814
display id: annotation2198814
version: 1
top level: BBa_K801043
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198814/range2198814/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198814/range2198814/1
type: Range
persistent identity: range2198814
display id: range2198814
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2246
end: 5704
orientation: inline

BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
type: SequenceAnnotation
persistent identity: annotation2198798
display id: annotation2198798
version: 1
top level: BBa_K801043
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198798/range2198798/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198798/range2198798/1
type: Range
persistent identity: range2198798
display id: range2198798
version: 1
direction: direction/forward
top level: BBa_K801043
start: 456
end: 1259
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198808
display id: annotation2198808
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198808/range2198808/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198808/range2198808/1
type: Range
persistent identity: range2198808
display id: range2198808
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2246
end: 2302
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198797
display id: annotation2198797
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198797/range2198797/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198797/range2198797/1
type: Range
persistent identity: range2198797
display id: range2198797
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1
end: 450
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198812
display id: annotation2198812
version: 1
top level: BBa_K801043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198812/range2198812/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198812/range2198812/1
type: Range
persistent identity: range2198812
display id: range2198812
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5722
end: 6228
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198805
display id: annotation2198805
version: 1
top level: BBa_K801043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198805/range2198805/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198805/range2198805/1
type: Range
persistent identity: range2198805
display id: range2198805
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1268
end: 1774
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198804
display id: annotation2198804
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198804/range2198804/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198804/range2198804/1
type: Range
persistent identity: range2198804
display id: range2198804
version: 1
direction: direction/forward
top level: BBa_K801043
start: 960
end: 1256
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation2198811
display id: annotation2198811
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198811/range2198811/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198811/range2198811/1
type: Range
persistent identity: range2198811
display id: range2198811
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5102
end: 5704
orientation: inline

BBa_K365005 (20 amino acids long linker)
type: SequenceAnnotation
persistent identity: annotation2198810
display id: annotation2198810
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198810/range2198810/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198810/range2198810/1
type: Range
persistent identity: range2198810
display id: range2198810
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5036
end: 5095
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198799
display id: annotation2198799
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198799/range2198799/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198799/range2198799/1
type: Range
persistent identity: range2198799
display id: range2198799
version: 1
direction: direction/forward
top level: BBa_K801043
start: 465
end: 521
orientation: inline

GAL4AD (GAL4 transcriptonal activation domain)
type: SequenceAnnotation
persistent identity: annotation2198800
display id: annotation2198800
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198800/range2198800/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198800/range2198800/1
type: Range
persistent identity: range2198800
display id: range2198800
version: 1
direction: direction/forward
top level: BBa_K801043
start: 525
end: 863
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2198802
display id: annotation2198802
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198802/range2198802/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198802/range2198802/1
type: Range
persistent identity: range2198802
display id: range2198802
version: 1
direction: direction/forward
top level: BBa_K801043
start: 869
end: 891
orientation: inline

Phytochrome B (PhyB, 908 first N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198809
display id: annotation2198809
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198809/range2198809/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198809/range2198809/1
type: Range
persistent identity: range2198809
display id: range2198809
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2309
end: 5029
orientation: inline

HA epitope tage
type: SequenceAnnotation
persistent identity: annotation2198803
display id: annotation2198803
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198803/range2198803/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198803/range2198803/1
type: Range
persistent identity: range2198803
display id: range2198803
version: 1
direction: direction/forward
top level: BBa_K801043
start: 912
end: 932
orientation: inline

SV40NLS-PhyB-Linker-LexA
type: SequenceAnnotation
persistent identity: annotation2198807
display id: annotation2198807
version: 1
top level: BBa_K801043
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198807/range2198807/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198807/range2198807/1
type: Range
persistent identity: range2198807
display id: range2198807
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2237
end: 5713
orientation: inline

VP64
type: ComponentDefinition
persistent identity: BBa K1982012
display id: BBa_K1982012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VP64 transcription activitor
creator: Zexu Li
created: 2016-10-17T11:00:00Z
modified: 2016-10-18T09:58:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" ! colspan="2" style="background:#FFBF00;"|VP64 |- |'''Function''' |transcription activator |- |'''RFC standard''' |[http://parts.igem.org/Help:Assembly_standard_10 RFC 10] |- |'''Backbone''' |pSB1C3 |- |'''Organism''' |''synthetic construct'' |- |'''Source''' |synthetic construct |- |'''Submitted by''' |[http://2016.igem.org/Team:NEU-China NEU-China 2016] |} Tetrameric VP16 transcription activator domain
mutable notes: synthetic construct
mutable provenance: synthetic construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: VP16
sequence annotation: FLAG
sequence annotation: HA
sequence annotation: Linker
sequence annotation: TAATAA
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K1982012_sequence/1

VP16
type: SequenceAnnotation
persistent identity: annotation2517152
display id: annotation2517152
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517152/range2517152/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517152/range2517152/1
type: Range
persistent identity: range2517152
display id: range2517152
version: 1
direction: direction/forward
top level: VP64
start: 103
end: 141
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2517156
display id: annotation2517156
version: 1
top level: VP64
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517156/range2517156/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517156/range2517156/1
type: Range
persistent identity: range2517156
display id: range2517156
version: 1
direction: direction/forward
top level: VP64
start: 217
end: 240
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517151
display id: annotation2517151
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517151/range2517151/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517151/range2517151/1
type: Range
persistent identity: range2517151
display id: range2517151
version: 1
direction: direction/forward
top level: VP64
start: 64
end: 102
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517153
display id: annotation2517153
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517153/range2517153/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517153/range2517153/1
type: Range
persistent identity: range2517153
display id: range2517153
version: 1
direction: direction/forward
top level: VP64
start: 142
end: 180
orientation: inline

HA
type: SequenceAnnotation
persistent identity: annotation2517155
display id: annotation2517155
version: 1
top level: VP64
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517155/range2517155/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517155/range2517155/1
type: Range
persistent identity: range2517155
display id: range2517155
version: 1
direction: direction/forward
top level: VP64
start: 187
end: 213
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2517154
display id: annotation2517154
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517154/range2517154/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517154/range2517154/1
type: Range
persistent identity: range2517154
display id: range2517154
version: 1
direction: direction/forward
top level: VP64
start: 181
end: 186
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2517157
display id: annotation2517157
version: 1
top level: VP64
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517157/range2517157/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517157/range2517157/1
type: Range
persistent identity: range2517157
display id: range2517157
version: 1
direction: direction/forward
top level: VP64
start: 241
end: 246
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517150
display id: annotation2517150
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517150/range2517150/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517150/range2517150/1
type: Range
persistent identity: range2517150
display id: range2517150
version: 1
direction: direction/forward
top level: VP64
start: 22
end: 63
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2517149
display id: annotation2517149
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517149/range2517149/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517149/range2517149/1
type: Range
persistent identity: range2517149
display id: range2517149
version: 1
direction: direction/forward
top level: VP64
start: 1
end: 21
orientation: inline

BBa_K352001
type: ComponentDefinition
persistent identity: BBa K352001
display id: BBa_K352001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CooA from Rhodospirillum rubrum
creator: Cihan Tastan
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _474_606_
m user id: 0
owner id: 5815
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. CooA is a member of a family of transcriptional regulators similar to the cAMP receptor protein and fumavate nitrate reduction from Escherichia coli. The protein is active in sequence-specific DNA binding in the presence of CO, but not in the absence of CO. The protein to be a dimer in the absence of CO. The product, CooA, is 28% identical (51% similar) to CRP(cAMP receptor protein) and 18% identical (45% similar) to FNR(fumavate nitrate reduction) from Escherichia coli. Inactive Fe(II) CooA structure adapted from that of the strain with PDB identification no. 1FT9. The protein consists of two monomers, shaded differently, which dimerize along the central C-helices of adjacent effector-binding domains. The solved structure is asymmetric, in which one monomer contains fused C- and D-helices. Nonetheless, both F-helices that interact with DNA in a sequence-specific manner are buried from the surface in the structure. The 4/5 loop is noted and so are the Pro2 and His77 heme Fe(II) ligands.
mutable notes: The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.
mutable provenance: Hwan Youn, Robert L. Kerby, Mary Conrad, et al. 2004. Functionally Critical Elements of CooA-Related CO Sensors. J. Bacteriol. 186(5):1320-1329. doi:10.1128/JB.186.5.1320-1329.2004.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K352001_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2081251
display id: annotation2081251
version: 1
top level: BBa_K352001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K352001/annotation2081251/range2081251/1

https://synbiohub.org/public/igem/BBa_K352001/annotation2081251/range2081251/1
type: Range
persistent identity: range2081251
display id: range2081251
version: 1
direction: direction/forward
top level: BBa_K352001
start: 1
end: 3
orientation: inline

BBa_K1333201
type: ComponentDefinition
persistent identity: BBa K1333201
display id: BBa_K1333201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda cI-LGF2(D78A)
creator: Yanwen Xie
created: 2014-09-08T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78. As one of the components in Bacterial Two-hybrid System, it causes a less interaction with GAL11P compared to the nomalized WT.
mutable notes: Mutation of GAC to GCC LGF2 at site 78.
mutable provenance: From Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG start codon
sequence annotation: TGA stop codon
sequence annotation: LGF2(D78A)
sequence annotation: lambda cI
sequence: https://synbiohub.org/public/igem/BBa_K1333201_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2382833
display id: annotation2382833
version: 1
top level: BBa_K1333201
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382833/range2382833/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382833/range2382833/1
type: Range
persistent identity: range2382833
display id: range2382833
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 1
end: 3
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2382834
display id: annotation2382834
version: 1
top level: BBa_K1333201
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382834/range2382834/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382834/range2382834/1
type: Range
persistent identity: range2382834
display id: range2382834
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 841
end: 843
orientation: inline

LGF2(D78A)
type: SequenceAnnotation
persistent identity: annotation2382832
display id: annotation2382832
version: 1
top level: BBa_K1333201
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382832/range2382832/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382832/range2382832/1
type: Range
persistent identity: range2382832
display id: range2382832
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 721
end: 840
orientation: inline

lambda cI
type: SequenceAnnotation
persistent identity: annotation2382831
display id: annotation2382831
version: 1
top level: BBa_K1333201
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382831/range2382831/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382831/range2382831/1
type: Range
persistent identity: range2382831
display id: range2382831
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 1
end: 711
orientation: inline

BBa_K801042
type: ComponentDefinition
persistent identity: BBa K801042
display id: BBa_K801042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GAL4 based yeast light-switchable promoter system
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011
mutable notes: -
mutable provenance: composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K319003 (pTEF1, TEF1 promoter)
sequence annotation: HA epitope tage
sequence annotation: SV40NLS-PhyB-Linker-GAL4DBD
sequence annotation: BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD)
sequence annotation: Linker
sequence annotation: GAL4DBD (GAL4 DNA binding domain)
sequence annotation: BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
sequence annotation: SV40 NLS (SV40 nuclear localization signal)
sequence annotation: BBa_K801011, (tTEF1, TEF1 terminator)
sequence annotation: T7 promoter (for sequencing)
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: Phytochrome B (PhyB, 908 first N-terminal amino acids)
sequence annotation: 20 amino acids long linker (BBa_K365005)
sequence annotation: GAL4AD (GAL4 transcriptonal activation domain)
sequence: https://synbiohub.org/public/igem/BBa_K801042_sequence/1

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198790
display id: annotation2198790
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198790/range2198790/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198790/range2198790/1
type: Range
persistent identity: range2198790
display id: range2198790
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1782
end: 2231
orientation: inline

HA epitope tage
type: SequenceAnnotation
persistent identity: annotation2198787
display id: annotation2198787
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198787/range2198787/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198787/range2198787/1
type: Range
persistent identity: range2198787
display id: range2198787
version: 1
direction: direction/forward
top level: BBa_K801042
start: 912
end: 932
orientation: inline

SV40NLS-PhyB-Linker-GAL4DBD
type: SequenceAnnotation
persistent identity: annotation2198791
display id: annotation2198791
version: 1
top level: BBa_K801042
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198791/range2198791/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198791/range2198791/1
type: Range
persistent identity: range2198791
display id: range2198791
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2237
end: 5548
orientation: inline

BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD)
type: SequenceAnnotation
persistent identity: annotation2198793
display id: annotation2198793
version: 1
top level: BBa_K801042
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198793/range2198793/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198793/range2198793/1
type: Range
persistent identity: range2198793
display id: range2198793
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2246
end: 5539
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2198785
display id: annotation2198785
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198785/range2198785/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198785/range2198785/1
type: Range
persistent identity: range2198785
display id: range2198785
version: 1
direction: direction/forward
top level: BBa_K801042
start: 864
end: 959
orientation: inline

GAL4DBD (GAL4 DNA binding domain)
type: SequenceAnnotation
persistent identity: annotation2198796
display id: annotation2198796
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198796/range2198796/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198796/range2198796/1
type: Range
persistent identity: range2198796
display id: range2198796
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5102
end: 5539
orientation: inline

BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
type: SequenceAnnotation
persistent identity: annotation2198782
display id: annotation2198782
version: 1
top level: BBa_K801042
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198782/range2198782/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198782/range2198782/1
type: Range
persistent identity: range2198782
display id: range2198782
version: 1
direction: direction/forward
top level: BBa_K801042
start: 456
end: 1259
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198781
display id: annotation2198781
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198781/range2198781/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198781/range2198781/1
type: Range
persistent identity: range2198781
display id: range2198781
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1
end: 450
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198783
display id: annotation2198783
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198783/range2198783/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198783/range2198783/1
type: Range
persistent identity: range2198783
display id: range2198783
version: 1
direction: direction/forward
top level: BBa_K801042
start: 465
end: 521
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198789
display id: annotation2198789
version: 1
top level: BBa_K801042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198789/range2198789/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198789/range2198789/1
type: Range
persistent identity: range2198789
display id: range2198789
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1268
end: 1774
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2198786
display id: annotation2198786
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198786/range2198786/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198786/range2198786/1
type: Range
persistent identity: range2198786
display id: range2198786
version: 1
direction: direction/forward
top level: BBa_K801042
start: 869
end: 891
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198788
display id: annotation2198788
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198788/range2198788/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198788/range2198788/1
type: Range
persistent identity: range2198788
display id: range2198788
version: 1
direction: direction/forward
top level: BBa_K801042
start: 960
end: 1256
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198792
display id: annotation2198792
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198792/range2198792/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198792/range2198792/1
type: Range
persistent identity: range2198792
display id: range2198792
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2246
end: 2302
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198816
display id: annotation2198816
version: 1
top level: BBa_K801042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198816/range2198816/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198816/range2198816/1
type: Range
persistent identity: range2198816
display id: range2198816
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5557
end: 6063
orientation: inline

Phytochrome B (PhyB, 908 first N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198794
display id: annotation2198794
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198794/range2198794/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198794/range2198794/1
type: Range
persistent identity: range2198794
display id: range2198794
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2309
end: 5029
orientation: inline

20 amino acids long linker (BBa_K365005)
type: SequenceAnnotation
persistent identity: annotation2198795
display id: annotation2198795
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198795/range2198795/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198795/range2198795/1
type: Range
persistent identity: range2198795
display id: range2198795
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5036
end: 5095
orientation: inline

GAL4AD (GAL4 transcriptonal activation domain)
type: SequenceAnnotation
persistent identity: annotation2198784
display id: annotation2198784
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198784/range2198784/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198784/range2198784/1
type: Range
persistent identity: range2198784
display id: range2198784
version: 1
direction: direction/forward
top level: BBa_K801042
start: 525
end: 863
orientation: inline

BBa_K1333200
type: ComponentDefinition
persistent identity: BBa K1333200
display id: BBa_K1333200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda cI-LGF2(E75A)
creator: Yanwen Xie
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fuse lambda cI with LGF2 mutated from Glu to Ala at site 75 (the Glu which locates after three continuous Ala behind lambda repressor is at site 68). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.
mutable notes: The LGF2 is mutated from GAA to GCA at site 75.
mutable provenance: It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lambda cI
sequence annotation: LGF2(E75A)
sequence annotation: ATG start codon
sequence annotation: TGA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1333200_sequence/1

lambda cI
type: SequenceAnnotation
persistent identity: annotation2385724
display id: annotation2385724
version: 1
top level: BBa_K1333200
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385724/range2385724/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385724/range2385724/1
type: Range
persistent identity: range2385724
display id: range2385724
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 1
end: 711
orientation: inline

LGF2(E75A)
type: SequenceAnnotation
persistent identity: annotation2385726
display id: annotation2385726
version: 1
top level: BBa_K1333200
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385726/range2385726/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385726/range2385726/1
type: Range
persistent identity: range2385726
display id: range2385726
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 721
end: 840
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2385725
display id: annotation2385725
version: 1
top level: BBa_K1333200
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385725/range2385725/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385725/range2385725/1
type: Range
persistent identity: range2385725
display id: range2385725
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 1
end: 3
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2385727
display id: annotation2385727
version: 1
top level: BBa_K1333200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385727/range2385727/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385727/range2385727/1
type: Range
persistent identity: range2385727
display id: range2385727
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 841
end: 843
orientation: inline

BBa_K1333203
type: ComponentDefinition
persistent identity: BBa K1333203
display id: BBa_K1333203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda cI-LGF2(D76A&D78A)
creator: Yanwen Xie
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fuse lambda cI with LGF2 mutated from Asp to Ala at sites 76 and 78 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.
mutable notes: The LGF2 is mutated from GAC to GCC at site 76 and from GAC to GCC at site 78.
mutable provenance: It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TGA stop codon
sequence annotation: LGF2(D76A&D78A)
sequence annotation: ATG start codon
sequence annotation: lambda cI
sequence: https://synbiohub.org/public/igem/BBa_K1333203_sequence/1

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2385733
display id: annotation2385733
version: 1
top level: BBa_K1333203
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1333203/annotation2385733/range2385733/1

https://synbiohub.org/public/igem/BBa_K1333203/annotation2385733/range2385733/1
type: Range
persistent identity: range2385733
display id: range2385733
version: 1
direction: direction/forward
top level: BBa_K1333203
start: 841
end: 843
orientation: inline

LGF2(D76A&D78A)
type: SequenceAnnotation
persistent identity: annotation2385735
display id: annotation2385735
version: 1
top level: BBa_K1333203
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333203/annotation2385735/range2385735/1

https://synbiohub.org/public/igem/BBa_K1333203/annotation2385735/range2385735/1
type: Range
persistent identity: range2385735
display id: range2385735
version: 1
direction: direction/forward
top level: BBa_K1333203
start: 721
end: 840
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2385732
display id: annotation2385732
version: 1
top level: BBa_K1333203
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1333203/annotation2385732/range2385732/1

https://synbiohub.org/public/igem/BBa_K1333203/annotation2385732/range2385732/1
type: Range
persistent identity: range2385732
display id: range2385732
version: 1
direction: direction/forward
top level: BBa_K1333203
start: 1
end: 3
orientation: inline

lambda cI
type: SequenceAnnotation
persistent identity: annotation2385734
display id: annotation2385734
version: 1
top level: BBa_K1333203
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333203/annotation2385734/range2385734/1

https://synbiohub.org/public/igem/BBa_K1333203/annotation2385734/range2385734/1
type: Range
persistent identity: range2385734
display id: range2385734
version: 1
direction: direction/forward
top level: BBa_K1333203
start: 1
end: 711
orientation: inline