//proteindomain/affinity
type: Collection
persistent identity: proteindomain affinity
display id: proteindomain_affinity
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: 3x FLAG
member: HA tag
member: BBa_T2025
member: BBa_K1404006
member: V5 tag
member: S-tag
member: BBa_K823034
member: GST
member: BBa_K404211
member: His
member: Strep-tag
member: S*Tag
member: His-Stop
member: FLAG
member: c-Myc
member: BBa_K112502
member: VSV-G tag
member: BBa_K823037
member: BBa_K1404008
member: BBa_J70620
member: HA
member: BBa_K112503
member: BBa_K1404007
member: BBa_K823036
member: BBa_K1319007
member: BBa_K112603
member: StrepII
member: BBa_K823035
member: Strep-Tag
member: HisTag
member: LT
member: BBa_K823038
member: 7x His
member: LigA
member: Met-His
member: BBa_K157012
member: BBa_T2028
member: BBa_K112601
member: RGD-His-St
member: BBa_K112602
member: 7xHis
member: BBa_K112507
member: S
member: His_FluA

S-tag
type: ComponentDefinition
persistent identity: BBa T2016
display id: BBa_T2016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-tag head domain (KETAAAKFERQHMDS)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a S-tag head domain designed to be at the N-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This head domain begins with Met-Lys to promote efficient translation of the protein in ''E. coli''.
mutable provenance: This is a synthetic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: preferred AAA +2 codon
sequence annotation: start codon
sequence annotation: S-tag
sequence: https://synbiohub.org/public/igem/BBa_T2016_sequence/1

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2018272
display id: annotation2018272
version: 1
top level: S-tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2016/annotation2018272/range2018272/1

https://synbiohub.org/public/igem/BBa_T2016/annotation2018272/range2018272/1
type: Range
persistent identity: range2018272
display id: range2018272
version: 1
direction: direction/forward
top level: S-tag
start: 4
end: 6
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2018271
display id: annotation2018271
version: 1
top level: S-tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2016/annotation2018271/range2018271/1

https://synbiohub.org/public/igem/BBa_T2016/annotation2018271/range2018271/1
type: Range
persistent identity: range2018271
display id: range2018271
version: 1
direction: direction/forward
top level: S-tag
start: 1
end: 3
orientation: inline

S-tag
type: SequenceAnnotation
persistent identity: annotation2018273
display id: annotation2018273
version: 1
top level: S-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2016/annotation2018273/range2018273/1

https://synbiohub.org/public/igem/BBa_T2016/annotation2018273/range2018273/1
type: Range
persistent identity: range2018273
display id: range2018273
version: 1
direction: direction/forward
top level: S-tag
start: 7
end: 51
orientation: inline

HA tag
type: ComponentDefinition
persistent identity: BBa T2011
display id: BBa_T2011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hemagglutinin (HA) tag special internal domain (YPYDVPDYA)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a HA tag special internal domain designed to be within a protein. HA tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This domain encodes the HA sequence YPYDVPDYA. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', C. elegans, ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: HA tag
sequence: https://synbiohub.org/public/igem/BBa_T2011_sequence/1

HA tag
type: SequenceAnnotation
persistent identity: annotation2017186
display id: annotation2017186
version: 1
top level: HA tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2011/annotation2017186/range2017186/1

https://synbiohub.org/public/igem/BBa_T2011/annotation2017186/range2017186/1
type: Range
persistent identity: range2017186
display id: range2017186
version: 1
direction: direction/forward
top level: HA tag
start: 1
end: 27
orientation: inline

HA tag
type: ComponentDefinition
persistent identity: BBa T2009
display id: BBa_T2009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hemagglutinin (HA) tag tail domain (YPYDVPDYA)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a HA tag tail domain designed to be at the C-terminus of a protein. HA tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This domain encodes the HA sequence YPYDVPDYA. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: HA tag
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_T2009_sequence/1

HA tag
type: SequenceAnnotation
persistent identity: annotation2017182
display id: annotation2017182
version: 1
top level: HA tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2009/annotation2017182/range2017182/1

https://synbiohub.org/public/igem/BBa_T2009/annotation2017182/range2017182/1
type: Range
persistent identity: range2017182
display id: range2017182
version: 1
direction: direction/forward
top level: HA tag
start: 1
end: 27
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2017183
display id: annotation2017183
version: 1
top level: HA tag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2009/annotation2017183/range2017183/1

https://synbiohub.org/public/igem/BBa_T2009/annotation2017183/range2017183/1
type: Range
persistent identity: range2017183
display id: range2017183
version: 1
direction: direction/forward
top level: HA tag
start: 28
end: 33
orientation: inline

S
type: ComponentDefinition
persistent identity: BBa K112705
display id: BBa_K112705
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S affinity tag
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This S-tag can be added between two protein parts. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of S-tag> basic part K112705 Wobble PCR dv013/dv014 (47 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+47, L) Product is pBca1256-K112705 ----------------------------------------------- dv013 Forward Biobricking of S-Tag> cgataGAATTCatgAGATCTatgaaagaaaccgctgctgctaaattcg dv014 Reverse Biobricking of S-Tag> cgttaGGATCCgctgtccatgtgctggcgttcgaatttagcagcagcgg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112705_sequence/1

BBa_J70620
type: ComponentDefinition
persistent identity: BBa J70620
display id: BBa_J70620
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFC12 Maltose Binding Protein Internal Domain [malE]
creator: Joseph Lynch
created: 2010-08-04T11:00:00Z
modified: 2015-05-08T01:08:25Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 6384
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This Maltose-binding protein is designed for either N-terminal or C-terminal fusions. MBP fused proteins can be purified in one step affinity chromatography using a cross-linked amylose matrix and then eluted with 10 mM maltose. Note that the amylose matrix can only be regenerated, in most cases, up to five times. The large size of this tag can also affect the fused protein. MBP fusions often have higher levels of expression and solubility that the native untagged protein, with typical yields of up to 40 mg/liter culture.
mutable notes: Primers:
mutable provenance: pMAL-c2x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Asparagine Spacer
sequence annotation: MBP
sequence: https://synbiohub.org/public/igem/BBa_J70620_sequence/1

Asparagine Spacer
type: SequenceAnnotation
persistent identity: annotation2076894
display id: annotation2076894
version: 1
top level: BBa_J70620
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J70620/annotation2076894/range2076894/1

https://synbiohub.org/public/igem/BBa_J70620/annotation2076894/range2076894/1
type: Range
persistent identity: range2076894
display id: range2076894
version: 1
direction: direction/forward
top level: BBa_J70620
start: 1094
end: 1138
orientation: inline

MBP
type: SequenceAnnotation
persistent identity: annotation2076870
display id: annotation2076870
version: 1
top level: BBa_J70620
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70620/annotation2076870/range2076870/1

https://synbiohub.org/public/igem/BBa_J70620/annotation2076870/range2076870/1
type: Range
persistent identity: range2076870
display id: range2076870
version: 1
direction: direction/forward
top level: BBa_J70620
start: 1
end: 1093
orientation: inline

FLAG
type: ComponentDefinition
persistent identity: BBa T2003
display id: BBa_T2003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FLAG octapeptide tail domain (DYKDDDDK)
creator: Reshma Shetty
created: 2006-10-25T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: kill this part
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: double stop codon
sequence annotation: FLAG tag octapeptide
sequence: https://synbiohub.org/public/igem/BBa_T2003_sequence/1

double stop codon
type: SequenceAnnotation
persistent identity: annotation2016711
display id: annotation2016711
version: 1
top level: FLAG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2003/annotation2016711/range2016711/1

https://synbiohub.org/public/igem/BBa_T2003/annotation2016711/range2016711/1
type: Range
persistent identity: range2016711
display id: range2016711
version: 1
direction: direction/forward
top level: FLAG
start: 25
end: 30
orientation: inline

FLAG tag octapeptide
type: SequenceAnnotation
persistent identity: annotation2016710
display id: annotation2016710
version: 1
top level: FLAG
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2003/annotation2016710/range2016710/1

https://synbiohub.org/public/igem/BBa_T2003/annotation2016710/range2016710/1
type: Range
persistent identity: range2016710
display id: range2016710
version: 1
direction: direction/forward
top level: FLAG
start: 1
end: 24
orientation: inline

FLAG
type: ComponentDefinition
persistent identity: BBa K128000
display id: BBa_K128000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FLAG affinity tag
creator: Sara Mouradian
created: 2008-07-09T11:00:00Z
modified: 2015-05-08T01:09:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _178_
m user id: 0
owner id: 2822
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the polypeptide protein FLAG tag. It is used in antibody binding assays if there is no antibody against the studied protein. It should be put at either the N terminus or the C terminus of the protein This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
mutable notes: Tags are almost always used in conjunction with another protein (the one being studied), so we used the modified Silver BioBrick prefix and suffix to make the construction of funcitonal fusion peptides possible
mutable provenance: This sequence is easily recognized by many antibodies.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Flag tag
sequence: https://synbiohub.org/public/igem/BBa_K128000_sequence/1

Flag tag
type: SequenceAnnotation
persistent identity: annotation1966459
display id: annotation1966459
version: 1
top level: FLAG
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K128000/annotation1966459/range1966459/1

https://synbiohub.org/public/igem/BBa_K128000/annotation1966459/range1966459/1
type: Range
persistent identity: range1966459
display id: range1966459
version: 1
direction: direction/forward
top level: FLAG
start: 1
end: 25
orientation: inline

BBa_K823034
type: ComponentDefinition
persistent identity: BBa K823034
display id: BBa_K823034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3x FLAG tag (Freiburg standard+RBS)
creator: Jara Radeck
created: 2012-09-06T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: 3x Flag tag with RBS in Freiburg standard. prefix: suffix:
mutable notes: For N- and C-terminal in-frame translational fusion to other genes in Freiburg standard.
mutable provenance: synthesis by gene art.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: FLAG
sequence: https://synbiohub.org/public/igem/BBa_K823034_sequence/1

FLAG
type: SequenceAnnotation
persistent identity: annotation2182331
display id: annotation2182331
version: 1
top level: BBa_K823034
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823034/annotation2182331/range2182331/1

https://synbiohub.org/public/igem/BBa_K823034/annotation2182331/range2182331/1
type: Range
persistent identity: range2182331
display id: range2182331
version: 1
direction: direction/forward
top level: BBa_K823034
start: 1
end: 21
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2182333
display id: annotation2182333
version: 1
top level: BBa_K823034
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823034/annotation2182333/range2182333/1

https://synbiohub.org/public/igem/BBa_K823034/annotation2182333/range2182333/1
type: Range
persistent identity: range2182333
display id: range2182333
version: 1
direction: direction/forward
top level: BBa_K823034
start: 43
end: 66
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2182332
display id: annotation2182332
version: 1
top level: BBa_K823034
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823034/annotation2182332/range2182332/1

https://synbiohub.org/public/igem/BBa_K823034/annotation2182332/range2182332/1
type: Range
persistent identity: range2182332
display id: range2182332
version: 1
direction: direction/forward
top level: BBa_K823034
start: 22
end: 42
orientation: inline

HA tag
type: ComponentDefinition
persistent identity: BBa K112506
display id: BBa_K112506
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < HA > in BBb
creator: Sherine Cheung
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is an HA tag without a start or a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<HA>} basic part K112506 Wobble PCR of cc001/cc010 ( 51 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112506 -------------------------------------------- cc001 Forward Biobricking of {<HA>} CgATAgaattcATGagatcttacccatacgacgtcccagac cc010 Reverse biobricking of {<HA>} ccagtggatcccccagcgtagtctgggacgtcgtatggg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112506_sequence/1

c-Myc
type: ComponentDefinition
persistent identity: BBa I712007
display id: BBa_I712007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c-Myc affinity tag
creator: Anja Korenčič
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: c-myc tag is used for detection of proteins by anti-c-myc antibodies.
mutable notes: Gene was designed to be constructed by primer annealing. Includes start codone.
mutable provenance: Constructed by primer annealing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_I712007_sequence/1

V5 tag
type: ComponentDefinition
persistent identity: BBa T2018
display id: BBa_T2018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: V5 epitope tag tail domain (GKPIPNPLLGLDST)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a V5 epitope tag tail domain designed to be at the C-terminus of a protein. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.
mutable notes: A double TAATAA stop codon was included. Rare codons from common model organisms were avoided.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: V5 epitope tag
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_T2018_sequence/1

V5 epitope tag
type: SequenceAnnotation
persistent identity: annotation2018275
display id: annotation2018275
version: 1
top level: V5 tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2018/annotation2018275/range2018275/1

https://synbiohub.org/public/igem/BBa_T2018/annotation2018275/range2018275/1
type: Range
persistent identity: range2018275
display id: range2018275
version: 1
direction: direction/forward
top level: V5 tag
start: 1
end: 42
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2018276
display id: annotation2018276
version: 1
top level: V5 tag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2018/annotation2018276/range2018276/1

https://synbiohub.org/public/igem/BBa_T2018/annotation2018276/range2018276/1
type: Range
persistent identity: range2018276
display id: range2018276
version: 1
direction: direction/forward
top level: V5 tag
start: 43
end: 48
orientation: inline

His
type: ComponentDefinition
persistent identity: BBa I757013
display id: BBa_I757013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His affinity tag
creator: Freiburg iGEM 2007 team
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _125_
m user id: 0
owner id: 2006
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: * His-tag * NgoMIV / AgeI protein fusion part * iGEM Team Freiburg 2007
mutable notes: between BioBrick 1.0 sites part is flanked 5' by NgoMIV and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions
mutable provenance: synthetic DNA by GeneArt
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence: https://synbiohub.org/public/igem/BBa_I757013_sequence/1

AgeI
type: SequenceAnnotation
persistent identity: annotation1954507
display id: annotation1954507
version: 1
top level: His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I757013/annotation1954507/range1954507/1

https://synbiohub.org/public/igem/BBa_I757013/annotation1954507/range1954507/1
type: Range
persistent identity: range1954507
display id: range1954507
version: 1
direction: direction/forward
top level: His
start: 28
end: 33
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation1954506
display id: annotation1954506
version: 1
top level: His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I757013/annotation1954506/range1954506/1

https://synbiohub.org/public/igem/BBa_I757013/annotation1954506/range1954506/1
type: Range
persistent identity: range1954506
display id: range1954506
version: 1
direction: direction/forward
top level: His
start: 4
end: 9
orientation: inline

HA
type: ComponentDefinition
persistent identity: BBa K128001
display id: BBa_K128001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HA affinity tag
creator: Sara Mouradian
created: 2008-07-09T11:00:00Z
modified: 2015-05-08T01:09:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _178_
m user id: 0
owner id: 2822
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the polypeptide protein HA tag. It is used in antibody binding assays if there is no antibody against the studied protein. It should be put at either the N terminus or the C terminus of the protein This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
mutable notes: Tags are almost always used in conjunction with another protein (the one being studied), so we used the modified Silver BioBrick prefix and suffix to make the construction of funcitonal fusion peptides possible
mutable provenance: This sequence is easily recognized by many antibodies.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: HA tag
sequence: https://synbiohub.org/public/igem/BBa_K128001_sequence/1

HA tag
type: SequenceAnnotation
persistent identity: annotation1966476
display id: annotation1966476
version: 1
top level: HA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K128001/annotation1966476/range1966476/1

https://synbiohub.org/public/igem/BBa_K128001/annotation1966476/range1966476/1
type: Range
persistent identity: range1966476
display id: range1966476
version: 1
direction: direction/forward
top level: HA
start: 1
end: 27
orientation: inline

BBa_K157012
type: ComponentDefinition
persistent identity: BBa K157012
display id: BBa_K157012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strep affinity tag II; Freiburg standard
creator: Kristian M??ller
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strep tag as NgoMIV / AgeI protein fusion part, fusion to proteins facilitates detection, purification, immobilization.
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Strep-Tag II
sequence: https://synbiohub.org/public/igem/BBa_K157012_sequence/1

Strep-Tag II
type: SequenceAnnotation
persistent identity: annotation2041866
display id: annotation2041866
version: 1
top level: BBa_K157012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157012/annotation2041866/range2041866/1

https://synbiohub.org/public/igem/BBa_K157012/annotation2041866/range2041866/1
type: Range
persistent identity: range2041866
display id: range2041866
version: 1
direction: direction/forward
top level: BBa_K157012
start: 1
end: 24
orientation: inline

Strep-Tag II
type: SequenceAnnotation
persistent identity: annotation2041865
display id: annotation2041865
version: 1
top level: BBa_K157012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157012/annotation2041865/range2041865/1

https://synbiohub.org/public/igem/BBa_K157012/annotation2041865/range2041865/1
type: Range
persistent identity: range2041865
display id: range2041865
version: 1
direction: direction/forward
top level: BBa_K157012
start: 1
end: 24
orientation: inline

His
type: ComponentDefinition
persistent identity: BBa K128005
display id: BBa_K128005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His affinity tag
creator: Prarthna Desai
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:09:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _178_
m user id: 0
owner id: 2826
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This codes for six Histidine residues to allow for His column protein purification. It will work best if put at either the N or C terminus of your protein construct. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
mutable notes: This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
mutable provenance: This sequence is easily recognized by many antibodies.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K128005_sequence/1

BBa_K1404006
type: ComponentDefinition
persistent identity: BBa K1404006
display id: BBa_K1404006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA, curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Beta sheet5
sequence annotation: Beta sheet1
sequence annotation: CsgG signal
sequence annotation: CsgA-WT
sequence annotation: mutation
sequence annotation: Beta sheet2
sequence annotation: Beta sheet3
sequence annotation: Sec signal
sequence annotation: RBS Paris 2009
sequence annotation: Beta sheet4
sequence annotation: Part:BBa_K342000
sequence: https://synbiohub.org/public/igem/BBa_K1404006_sequence/1

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2425863
display id: annotation2425863
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1
type: Range
persistent identity: range2425863
display id: range2425863
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 488
end: 544
orientation: inline

Beta sheet1
type: SequenceAnnotation
persistent identity: annotation2425859
display id: annotation2425859
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1
type: Range
persistent identity: range2425859
display id: range2425859
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 218
end: 286
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2425825
display id: annotation2425825
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1
type: Range
persistent identity: range2425825
display id: range2425825
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 152
end: 217
orientation: inline

CsgA-WT
type: SequenceAnnotation
persistent identity: annotation2425824
display id: annotation2425824
version: 1
top level: BBa_K1404006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1
type: Range
persistent identity: range2425824
display id: range2425824
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 547
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2425844
display id: annotation2425844
version: 1
top level: BBa_K1404006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1
type: Range
persistent identity: range2425844
display id: range2425844
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 461
end: 463
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2425860
display id: annotation2425860
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1
type: Range
persistent identity: range2425860
display id: range2425860
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 287
end: 352
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2425861
display id: annotation2425861
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1
type: Range
persistent identity: range2425861
display id: range2425861
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 353
end: 421
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2425823
display id: annotation2425823
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1
type: Range
persistent identity: range2425823
display id: range2425823
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 151
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2425875
display id: annotation2425875
version: 1
top level: BBa_K1404006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1
type: Range
persistent identity: range2425875
display id: range2425875
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 79
end: 91
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2425862
display id: annotation2425862
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1
type: Range
persistent identity: range2425862
display id: range2425862
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 422
end: 487
orientation: inline

Part:BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2425822
display id: annotation2425822
version: 1
top level: BBa_K1404006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1
type: Range
persistent identity: range2425822
display id: range2425822
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 1
end: 70
orientation: inline

GST
type: ComponentDefinition
persistent identity: BBa J18917
display id: BBa_J18917
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GST tag (E. coli)
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: GST domain for protein purification. Note: GST is naturally forming dimers.
mutable notes: * aa sequence taken from vector pGEX * codon optimized for E. coli * gene synthesis
mutable provenance: pGEX vector (amino acid sequence only)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J18917_sequence/1

BBa_K404211
type: ComponentDefinition
persistent identity: BBa K404211
display id: BBa_K404211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587KO-His-Tag
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: TG Spacer
sequence annotation: upstream 587
sequence annotation: downstream 453
sequence annotation: GLS Spacer
sequence annotation: upstream 453
sequence annotation: downstream 587
sequence annotation: R=>A
sequence annotation: His-Tag
sequence: https://synbiohub.org/public/igem/BBa_K404211_sequence/1

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2079134
display id: annotation2079134
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079134/range2079134/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079134/range2079134/1
type: Range
persistent identity: range2079134
display id: range2079134
version: 1
direction: direction/forward
top level: BBa_K404211
start: 43
end: 48
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079132
display id: annotation2079132
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079132/range2079132/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079132/range2079132/1
type: Range
persistent identity: range2079132
display id: range2079132
version: 1
direction: direction/forward
top level: BBa_K404211
start: 10
end: 42
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079139
display id: annotation2079139
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079139/range2079139/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079139/range2079139/1
type: Range
persistent identity: range2079139
display id: range2079139
version: 1
direction: direction/forward
top level: BBa_K404211
start: 97
end: 105
orientation: inline

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2079136
display id: annotation2079136
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079136/range2079136/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079136/range2079136/1
type: Range
persistent identity: range2079136
display id: range2079136
version: 1
direction: direction/forward
top level: BBa_K404211
start: 67
end: 75
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079131
display id: annotation2079131
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079131/range2079131/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079131/range2079131/1
type: Range
persistent identity: range2079131
display id: range2079131
version: 1
direction: direction/forward
top level: BBa_K404211
start: 1
end: 9
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079138
display id: annotation2079138
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079138/range2079138/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079138/range2079138/1
type: Range
persistent identity: range2079138
display id: range2079138
version: 1
direction: direction/forward
top level: BBa_K404211
start: 76
end: 96
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079137
display id: annotation2079137
version: 1
top level: BBa_K404211
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079137/range2079137/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079137/range2079137/1
type: Range
persistent identity: range2079137
display id: range2079137
version: 1
direction: direction/forward
top level: BBa_K404211
start: 76
end: 78
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2079135
display id: annotation2079135
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079135/range2079135/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079135/range2079135/1
type: Range
persistent identity: range2079135
display id: range2079135
version: 1
direction: direction/forward
top level: BBa_K404211
start: 49
end: 66
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079133
display id: annotation2079133
version: 1
top level: BBa_K404211
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079133/range2079133/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079133/range2079133/1
type: Range
persistent identity: range2079133
display id: range2079133
version: 1
direction: direction/forward
top level: BBa_K404211
start: 34
end: 36
orientation: inline

BBa_K112603
type: ComponentDefinition
persistent identity: BBa K112603
display id: BBa_K112603
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < FLAG tag ! in BBb
creator: Sherine Cheung
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a FLAG tag with a stop codon, but no start codon. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<FLAG!} basic part K112603 Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 910) Product is pBca1256-K112603 ----------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag! cgataGAATTCatgAGATCTgactacaaggatgacgacg SC006 Reverse Biobricking of <FLAG-tag! ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112603_sequence/1

BBa_K823035
type: ComponentDefinition
persistent identity: BBa K823035
display id: BBa_K823035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HA-tag (Freiburg standard+RBS)
creator: Jara Radeck
created: 2012-09-06T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: HA-tag with RBS in Freiburg standard. prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
mutable notes: For N- and C-terminal in-frame translational fusion to other genes in Freiburg standard.
mutable provenance: gene synthesis by gene art.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K823035_sequence/1

BBa_T2028
type: ComponentDefinition
persistent identity: BBa T2028
display id: BBa_T2028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chitin binding internal domain
creator: Reshma Shetty
created: 2009-09-16T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a chitin binding domain (CBD) designed to be at between protein domains. Chitin binding domains are a type of affinity tag that can be used to purify proteins.
mutable notes: Rare codons from common model organisms as well as restriction sites used in various assembly standards were removed.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: chitin binding domain
sequence: https://synbiohub.org/public/igem/BBa_T2028_sequence/1

chitin binding domain
type: SequenceAnnotation
persistent identity: annotation2021347
display id: annotation2021347
version: 1
top level: BBa_T2028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_T2028/annotation2021347/range2021347/1

https://synbiohub.org/public/igem/BBa_T2028/annotation2021347/range2021347/1
type: Range
persistent identity: range2021347
display id: range2021347
version: 1
direction: direction/forward
top level: BBa_T2028
start: 1
end: 156
orientation: inline

V5 tag
type: ComponentDefinition
persistent identity: BBa T2019
display id: BBa_T2019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: V5 epitope tag head domain (GKPIPNPLLGLDST)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a V5 epitope tag head domain designed to be at the N-terminus of a protein. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.
mutable notes: This head domain begins with Met-Lys to promote efficient translation of the protein in E. coli. Rare codons from various model organisms were avoided where possible.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: V5 epitope tag
sequence annotation: preferred AAA +2 codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_T2019_sequence/1

V5 epitope tag
type: SequenceAnnotation
persistent identity: annotation2018279
display id: annotation2018279
version: 1
top level: V5 tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2019/annotation2018279/range2018279/1

https://synbiohub.org/public/igem/BBa_T2019/annotation2018279/range2018279/1
type: Range
persistent identity: range2018279
display id: range2018279
version: 1
direction: direction/forward
top level: V5 tag
start: 7
end: 48
orientation: inline

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2018278
display id: annotation2018278
version: 1
top level: V5 tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2019/annotation2018278/range2018278/1

https://synbiohub.org/public/igem/BBa_T2019/annotation2018278/range2018278/1
type: Range
persistent identity: range2018278
display id: range2018278
version: 1
direction: direction/forward
top level: V5 tag
start: 4
end: 6
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2018277
display id: annotation2018277
version: 1
top level: V5 tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2019/annotation2018277/range2018277/1

https://synbiohub.org/public/igem/BBa_T2019/annotation2018277/range2018277/1
type: Range
persistent identity: range2018277
display id: range2018277
version: 1
direction: direction/forward
top level: V5 tag
start: 1
end: 3
orientation: inline

Strep-tag
type: ComponentDefinition
persistent identity: BBa T2013
display id: BBa_T2013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strep-tag II head domain (WSHPQFEK)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a Strep tag head domain designed to be at the N-terminus of a protein. Strep tags are typically used for detection, purification and immobilization of proteins.
mutable notes: This domain encodes the Strep tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: start codon
sequence annotation: strep tag II
sequence annotation: preferred AAA +2 codon
sequence: https://synbiohub.org/public/igem/BBa_T2013_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2017309
display id: annotation2017309
version: 1
top level: Strep-tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2013/annotation2017309/range2017309/1

https://synbiohub.org/public/igem/BBa_T2013/annotation2017309/range2017309/1
type: Range
persistent identity: range2017309
display id: range2017309
version: 1
direction: direction/forward
top level: Strep-tag
start: 1
end: 3
orientation: inline

strep tag II
type: SequenceAnnotation
persistent identity: annotation2017311
display id: annotation2017311
version: 1
top level: Strep-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2013/annotation2017311/range2017311/1

https://synbiohub.org/public/igem/BBa_T2013/annotation2017311/range2017311/1
type: Range
persistent identity: range2017311
display id: range2017311
version: 1
direction: direction/forward
top level: Strep-tag
start: 7
end: 30
orientation: inline

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2017310
display id: annotation2017310
version: 1
top level: Strep-tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2013/annotation2017310/range2017310/1

https://synbiohub.org/public/igem/BBa_T2013/annotation2017310/range2017310/1
type: Range
persistent identity: range2017310
display id: range2017310
version: 1
direction: direction/forward
top level: Strep-tag
start: 4
end: 6
orientation: inline

BBa_K112602
type: ComponentDefinition
persistent identity: BBa K112602
display id: BBa_K112602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < FLAG tag > in BBb
creator: Sherine Cheung
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is FLAG tag with no start and no stop codon ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<FLAG>} basic part K112602 Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 910) Product is pBca1256-K112602 --------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag> cgataGAATTCatgAGATCTgactacaaggatgacgacg SC005 Reverse Biobricking of <FLAG-tag> ccagtGGATCCcttgtcgtcgtcatccttgtagtc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112602_sequence/1

V5 tag
type: ComponentDefinition
persistent identity: BBa T2020
display id: BBa_T2020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: V5 epitope tag special internal domain (GKPIPNPLLGLDST)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a V5-tag special internal domain designed to between protein domains. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.
mutable notes: Rare codons from common model organisms were avoided.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: V5 epitope tag
sequence: https://synbiohub.org/public/igem/BBa_T2020_sequence/1

V5 epitope tag
type: SequenceAnnotation
persistent identity: annotation2018280
display id: annotation2018280
version: 1
top level: V5 tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2020/annotation2018280/range2018280/1

https://synbiohub.org/public/igem/BBa_T2020/annotation2018280/range2018280/1
type: Range
persistent identity: range2018280
display id: range2018280
version: 1
direction: direction/forward
top level: V5 tag
start: 1
end: 42
orientation: inline

c-Myc
type: ComponentDefinition
persistent identity: BBa T2007
display id: BBa_T2007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c-Myc head domain (EQKLISEEDL)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a c-Myc tag head domain designed to be at the N-terminus of a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This tail domain encodes the c-Myc sequence EQKLISEEDL. The codons at each amino acid position were selected to avoid rare codons in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: start codon
sequence annotation: preferred AAA +2 codon
sequence annotation: c-Myc tag
sequence: https://synbiohub.org/public/igem/BBa_T2007_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2017179
display id: annotation2017179
version: 1
top level: c-Myc
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2007/annotation2017179/range2017179/1

https://synbiohub.org/public/igem/BBa_T2007/annotation2017179/range2017179/1
type: Range
persistent identity: range2017179
display id: range2017179
version: 1
direction: direction/forward
top level: c-Myc
start: 1
end: 3
orientation: inline

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2017307
display id: annotation2017307
version: 1
top level: c-Myc
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2007/annotation2017307/range2017307/1

https://synbiohub.org/public/igem/BBa_T2007/annotation2017307/range2017307/1
type: Range
persistent identity: range2017307
display id: range2017307
version: 1
direction: direction/forward
top level: c-Myc
start: 4
end: 6
orientation: inline

c-Myc tag
type: SequenceAnnotation
persistent identity: annotation2017180
display id: annotation2017180
version: 1
top level: c-Myc
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2007/annotation2017180/range2017180/1

https://synbiohub.org/public/igem/BBa_T2007/annotation2017180/range2017180/1
type: Range
persistent identity: range2017180
display id: range2017180
version: 1
direction: direction/forward
top level: c-Myc
start: 7
end: 36
orientation: inline

3x FLAG
type: ComponentDefinition
persistent identity: BBa J64005
display id: BBa_J64005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3x FLAG affinity tag
creator: Dan Widmaier
created: 2008-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A 3 repeat FLAG (DYKDDDDK) antigen epitope tag used for marking proteins of interest. Proteins carrying this tag can be easily detected using commercial western blotting reagents.
mutable notes: This sequence was designed as part of an oligonucleotide primer on each strand then annealed and ligated into the vector.
mutable provenance: Synthetic DNA from Oligonucleotide synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: STOP Codon
sequence annotation: 3x FLAG Tag
sequence annotation: XbaI Restriction Site
sequence: https://synbiohub.org/public/igem/BBa_J64005_sequence/1

STOP Codon
type: SequenceAnnotation
persistent identity: annotation1959596
display id: annotation1959596
version: 1
top level: 3x FLAG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J64005/annotation1959596/range1959596/1

https://synbiohub.org/public/igem/BBa_J64005/annotation1959596/range1959596/1
type: Range
persistent identity: range1959596
display id: range1959596
version: 1
direction: direction/forward
top level: 3x FLAG
start: 73
end: 75
orientation: inline

3x FLAG Tag
type: SequenceAnnotation
persistent identity: annotation1959595
display id: annotation1959595
version: 1
top level: 3x FLAG
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J64005/annotation1959595/range1959595/1

https://synbiohub.org/public/igem/BBa_J64005/annotation1959595/range1959595/1
type: Range
persistent identity: range1959595
display id: range1959595
version: 1
direction: direction/forward
top level: 3x FLAG
start: 1
end: 72
orientation: inline

XbaI Restriction Site
type: SequenceAnnotation
persistent identity: annotation1960214
display id: annotation1960214
version: 1
top level: 3x FLAG
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64005/annotation1960214/range1960214/1

https://synbiohub.org/public/igem/BBa_J64005/annotation1960214/range1960214/1
type: Range
persistent identity: range1960214
display id: range1960214
version: 1
direction: direction/forward
top level: 3x FLAG
start: 76
end: 81
orientation: inline

FLAG
type: ComponentDefinition
persistent identity: BBa T2005
display id: BBa_T2005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FLAG octapeptide special internal domain (DYKDDDDK)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a FLAG tag octapeptide special internal domain designed to be internal to the sequence of a protein. FLAG tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: The FLAG sequence AspTyrLysAspAspAspAspLys (DYKDDDDK) is one of the most commonly used FLAG tag sequences. Each of the amino acids in this octapeptide have two possible codon choices. For each amino acid, neither of the two codon choices appear to be rare in the common model organisms. So codons were selected to minimize repeats.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: FLAG octapeptide
sequence: https://synbiohub.org/public/igem/BBa_T2005_sequence/1

FLAG octapeptide
type: SequenceAnnotation
persistent identity: annotation2017176
display id: annotation2017176
version: 1
top level: FLAG
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2005/annotation2017176/range2017176/1

https://synbiohub.org/public/igem/BBa_T2005/annotation2017176/range2017176/1
type: Range
persistent identity: range2017176
display id: range2017176
version: 1
direction: direction/forward
top level: FLAG
start: 1
end: 24
orientation: inline

His
type: ComponentDefinition
persistent identity: BBa K112703
display id: BBa_K112703
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His affinity tag
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This his tag can be fused to the N terminus of a protein. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of His-Tag> basic part K112703 Mix dv009/dv010 (41bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+41, L) Product is pBca1256-K112703 ----------------------------------------------- dv009 Forward Biobricking of His-Tag> cgataGAATTCatgAGATCTatgCATCATCATCATCATCATGGATCCtaacg dv010 Reverse Biobricking of His-Tag> cgttaGGATCCATGATGATGATGATGATGcatAGATCTcatGAATTCtatgg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112703_sequence/1

VSV-G tag
type: ComponentDefinition
persistent identity: BBa T2021
display id: BBa_T2021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VSV-G epitope tag tail domain (YTDIEMNRLGK)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a VSV-G epitope tag tail domain designed to be at the C-terminus of a protein. VSV-G tags are a type of epitope tag that can be used to measure the quantity of proteins.
mutable notes: A double TAA TAA stop codon was used. Rare codons from model organisms were avoided where possible.
mutable provenance: This is a synthetic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: double TAA stop codon
sequence annotation: VSV-G epitope tag
sequence: https://synbiohub.org/public/igem/BBa_T2021_sequence/1

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2018282
display id: annotation2018282
version: 1
top level: VSV-G tag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2021/annotation2018282/range2018282/1

https://synbiohub.org/public/igem/BBa_T2021/annotation2018282/range2018282/1
type: Range
persistent identity: range2018282
display id: range2018282
version: 1
direction: direction/forward
top level: VSV-G tag
start: 34
end: 39
orientation: inline

VSV-G epitope tag
type: SequenceAnnotation
persistent identity: annotation2018281
display id: annotation2018281
version: 1
top level: VSV-G tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2021/annotation2018281/range2018281/1

https://synbiohub.org/public/igem/BBa_T2021/annotation2018281/range2018281/1
type: Range
persistent identity: range2018281
display id: range2018281
version: 1
direction: direction/forward
top level: VSV-G tag
start: 1
end: 33
orientation: inline

VSV-G tag
type: ComponentDefinition
persistent identity: BBa T2022
display id: BBa_T2022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VSV-G epitope tag special internal domain (YTDIEMNRLGK)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a VSV-G epitope tag special internal domain designed to be used between protein domains. VSV-G tags are a type of epitope tag that can be used to measure the quantity of proteins.
mutable notes: Rare codons from model organisms were avoided where possible.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: VSV-G tag
sequence: https://synbiohub.org/public/igem/BBa_T2022_sequence/1

VSV-G tag
type: SequenceAnnotation
persistent identity: annotation2018283
display id: annotation2018283
version: 1
top level: VSV-G tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2022/annotation2018283/range2018283/1

https://synbiohub.org/public/igem/BBa_T2022/annotation2018283/range2018283/1
type: Range
persistent identity: range2018283
display id: range2018283
version: 1
direction: direction/forward
top level: VSV-G tag
start: 1
end: 33
orientation: inline

Strep-tag
type: ComponentDefinition
persistent identity: BBa T2012
display id: BBa_T2012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strep-tag tail domain (WRHPQFGG)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a Strep tag tail domain designed to be at the C-terminus of a protein. Strep tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This domain encodes the Strep tag sequence AWRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Strep-tag
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_T2012_sequence/1

Strep-tag
type: SequenceAnnotation
persistent identity: annotation2017187
display id: annotation2017187
version: 1
top level: Strep-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2012/annotation2017187/range2017187/1

https://synbiohub.org/public/igem/BBa_T2012/annotation2017187/range2017187/1
type: Range
persistent identity: range2017187
display id: range2017187
version: 1
direction: direction/forward
top level: Strep-tag
start: 1
end: 24
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2017188
display id: annotation2017188
version: 1
top level: Strep-tag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2012/annotation2017188/range2017188/1

https://synbiohub.org/public/igem/BBa_T2012/annotation2017188/range2017188/1
type: Range
persistent identity: range2017188
display id: range2017188
version: 1
direction: direction/forward
top level: Strep-tag
start: 25
end: 30
orientation: inline

7xHis
type: ComponentDefinition
persistent identity: BBa T2002
display id: BBa_T2002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 7x His tag special internal domain
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a 6x His tag special internal domain is designed to be between protein domains. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: Used both Histidine codons to minimize repeats.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: 7x His
sequence: https://synbiohub.org/public/igem/BBa_T2002_sequence/1

7x His
type: SequenceAnnotation
persistent identity: annotation2016499
display id: annotation2016499
version: 1
top level: 7xHis
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2002/annotation2016499/range2016499/1

https://synbiohub.org/public/igem/BBa_T2002/annotation2016499/range2016499/1
type: Range
persistent identity: range2016499
display id: range2016499
version: 1
direction: direction/forward
top level: 7xHis
start: 1
end: 21
orientation: inline

BBa_K112507
type: ComponentDefinition
persistent identity: BBa K112507
display id: BBa_K112507
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < HA ! in BBb
creator: Sherine Cheung
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is an HA tag without a start, but with a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<HA!} basic part K112507 Wobble PCR of cc001/cc011 ( 54 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112507 -------------------------------------------- cc001 Forward Biobricking of {<HA>} CgATAgaattcATGagatcttacccatacgacgtcccagac cc011 Reverse biobricking of {<HA!} ccagtggatccttacccagcgtagtctgggacgtcgtatgggtaag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112507_sequence/1

7x His
type: ComponentDefinition
persistent identity: BBa T2001
display id: BBa_T2001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 7x His tag head domain
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a 6x His tag head domain designed to encode the N-terminus of a protein. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: Both Histidine codons were used to minimize repeats. An ATG start codon was used.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: preferred AAA +2 codon
sequence annotation: start codon
sequence annotation: 7x his tag
sequence: https://synbiohub.org/public/igem/BBa_T2001_sequence/1

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2017305
display id: annotation2017305
version: 1
top level: 7x His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2001/annotation2017305/range2017305/1

https://synbiohub.org/public/igem/BBa_T2001/annotation2017305/range2017305/1
type: Range
persistent identity: range2017305
display id: range2017305
version: 1
direction: direction/forward
top level: 7x His
start: 4
end: 6
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2016497
display id: annotation2016497
version: 1
top level: 7x His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2001/annotation2016497/range2016497/1

https://synbiohub.org/public/igem/BBa_T2001/annotation2016497/range2016497/1
type: Range
persistent identity: range2016497
display id: range2016497
version: 1
direction: direction/forward
top level: 7x His
start: 1
end: 3
orientation: inline

7x his tag
type: SequenceAnnotation
persistent identity: annotation2016498
display id: annotation2016498
version: 1
top level: 7x His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2001/annotation2016498/range2016498/1

https://synbiohub.org/public/igem/BBa_T2001/annotation2016498/range2016498/1
type: Range
persistent identity: range2016498
display id: range2016498
version: 1
direction: direction/forward
top level: 7x His
start: 7
end: 27
orientation: inline

HisTag
type: ComponentDefinition
persistent identity: BBa J18909
display id: BBa_J18909
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His tag (6xHis; E. coli - optimized)
creator: Raik Gruenberg
created: 2010-01-15T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Hexahistidine affinity tag. Binds zinc, nickel, or cobalt affinity resins. =Notes= This part is identical to BBa_I757013 (which does not seem to be available though). =References= Wikipedia article: [http://en.wikipedia.org/wiki/Polyhistidine-tag]
mutable notes: Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: PolyHis
sequence: https://synbiohub.org/public/igem/BBa_J18909_sequence/1

PolyHis
type: SequenceAnnotation
persistent identity: annotation2065463
display id: annotation2065463
version: 1
top level: HisTag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1

https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
type: Range
persistent identity: range2065463
display id: range2065463
version: 1
direction: direction/forward
top level: HisTag
start: 1
end: 18
orientation: inline

7x His
type: ComponentDefinition
persistent identity: BBa T2000
display id: BBa_T2000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 7x His tag tail domain
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a 6x His tag tail domain designed to encode the C-terminus of a protein. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: Used both His codons in order to avoid repeats. Also included a double TAATAA stop codon.
mutable provenance: No. This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: double stop codon
sequence annotation: 7x His
sequence: https://synbiohub.org/public/igem/BBa_T2000_sequence/1

double stop codon
type: SequenceAnnotation
persistent identity: annotation2016496
display id: annotation2016496
version: 1
top level: 7x His
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2000/annotation2016496/range2016496/1

https://synbiohub.org/public/igem/BBa_T2000/annotation2016496/range2016496/1
type: Range
persistent identity: range2016496
display id: range2016496
version: 1
direction: direction/forward
top level: 7x His
start: 22
end: 27
orientation: inline

7x His
type: SequenceAnnotation
persistent identity: annotation2016495
display id: annotation2016495
version: 1
top level: 7x His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2000/annotation2016495/range2016495/1

https://synbiohub.org/public/igem/BBa_T2000/annotation2016495/range2016495/1
type: Range
persistent identity: range2016495
display id: range2016495
version: 1
direction: direction/forward
top level: 7x His
start: 1
end: 21
orientation: inline

LT
type: ComponentDefinition
persistent identity: BBa K2144101
display id: BBa_K2144101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Vector containing Hisx6 and Sortase tag LPETGG
creator: Sigrun Stulz
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T01:15:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2614_
m user id: 31510
owner id: 31510
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This vector is constructed to simplify the usage of the transpeptidase Sortase A for future iGEM teams. Following a BamHI restriction site we constructed a synthetic sequence consisting of three main elements: 1) A linker (GSSS) to allow for limited mobility of the attached peptides and facilitate correct folding 2) A Sortase A tag (LPETGG) that is used to conjugate the proteins to poly-glycines 3) A standard Hisx6 tag commonly used for Immobilized Metal Affinity Chromatography (IMAC) This sequence follows a BamHI restriction site which can be used by teams to clone in whichever peptide they would like to conjugate using Sortase A.
mutable notes: BamHI is a very convenient restriction enzyme for our application as its restriction site will code for a glycine and a serine, both of which are often used in linkers and tend to be unproblematic in such sequences.
mutable provenance: The plasmid was constructed using the iGEM-supplied pSB1C3 and a synthetically produced sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: barcode
sequence annotation: -35
sequence annotation: LacI
sequence annotation: Hisx6
sequence annotation: polya
sequence annotation: stem_loop
sequence annotation: BBa_R0010
sequence annotation: -10
sequence annotation: T7 TE
sequence annotation: ori
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: stop
sequence annotation: Sortase A Tag
sequence annotation: conserved
sequence annotation: Stop
sequence annotation: CAP
sequence annotation: CmR
sequence annotation: BBa_B0034
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K2144101_sequence/1

barcode
type: SequenceAnnotation
persistent identity: annotation2509044
display id: annotation2509044
version: 1
top level: LT
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509044/range2509044/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509044/range2509044/1
type: Range
persistent identity: range2509044
display id: range2509044
version: 1
direction: direction/forward
top level: LT
start: 908
end: 932
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2509037
display id: annotation2509037
version: 1
top level: LT
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509037/range2509037/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509037/range2509037/1
type: Range
persistent identity: range2509037
display id: range2509037
version: 1
direction: direction/forward
top level: LT
start: 137
end: 142
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation2509039
display id: annotation2509039
version: 1
top level: LT
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509039/range2509039/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509039/range2509039/1
type: Range
persistent identity: range2509039
display id: range2509039
version: 1
direction: direction/forward
top level: LT
start: 166
end: 200
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2509052
display id: annotation2509052
version: 1
top level: LT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509052/range2509052/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509052/range2509052/1
type: Range
persistent identity: range2509052
display id: range2509052
version: 1
direction: direction/forward
top level: LT
start: 2077
end: 2094
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2509049
display id: annotation2509049
version: 1
top level: LT
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509049/range2509049/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509049/range2509049/1
type: Range
persistent identity: range2509049
display id: range2509049
version: 1
direction: direction/forward
top level: LT
start: 1056
end: 1069
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2509046
display id: annotation2509046
version: 1
top level: LT
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509046/range2509046/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509046/range2509046/1
type: Range
persistent identity: range2509046
display id: range2509046
version: 1
direction: direction/forward
top level: LT
start: 952
end: 995
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2509035
display id: annotation2509035
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509035/range2509035/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509035/range2509035/1
type: Range
persistent identity: range2509035
display id: range2509035
version: 1
direction: direction/forward
top level: LT
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2509038
display id: annotation2509038
version: 1
top level: LT
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509038/range2509038/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509038/range2509038/1
type: Range
persistent identity: range2509038
display id: range2509038
version: 1
direction: direction/forward
top level: LT
start: 161
end: 166
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2509048
display id: annotation2509048
version: 1
top level: LT
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509048/range2509048/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509048/range2509048/1
type: Range
persistent identity: range2509048
display id: range2509048
version: 1
direction: direction/forward
top level: LT
start: 1036
end: 1055
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2509043
display id: annotation2509043
version: 1
top level: LT
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509043/range2509043/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509043/range2509043/1
type: Range
persistent identity: range2509043
display id: range2509043
version: 1
direction: direction/forward
top level: LT
start: 254
end: 842
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2509045
display id: annotation2509045
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509045/range2509045/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509045/range2509045/1
type: Range
persistent identity: range2509045
display id: range2509045
version: 1
direction: direction/forward
top level: LT
start: 941
end: 1020
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2509047
display id: annotation2509047
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509047/range2509047/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509047/range2509047/1
type: Range
persistent identity: range2509047
display id: range2509047
version: 1
direction: direction/forward
top level: LT
start: 1029
end: 1069
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2509050
display id: annotation2509050
version: 1
top level: LT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509050/range2509050/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509050/range2509050/1
type: Range
persistent identity: range2509050
display id: range2509050
version: 1
direction: direction/forward
top level: LT
start: 1062
end: 1062
orientation: inline

Sortase A Tag
type: SequenceAnnotation
persistent identity: annotation2509053
display id: annotation2509053
version: 1
top level: LT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509053/range2509053/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509053/range2509053/1
type: Range
persistent identity: range2509053
display id: range2509053
version: 1
direction: direction/forward
top level: LT
start: 2059
end: 2076
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2509042
display id: annotation2509042
version: 1
top level: LT
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509042/range2509042/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509042/range2509042/1
type: Range
persistent identity: range2509042
display id: range2509042
version: 1
direction: direction/forward
top level: LT
start: 213
end: 216
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2509054
display id: annotation2509054
version: 1
top level: LT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509054/range2509054/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509054/range2509054/1
type: Range
persistent identity: range2509054
display id: range2509054
version: 1
direction: direction/forward
top level: LT
start: 2095
end: 2097
orientation: inline

CAP
type: SequenceAnnotation
persistent identity: annotation2509036
display id: annotation2509036
version: 1
top level: LT
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509036/range2509036/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509036/range2509036/1
type: Range
persistent identity: range2509036
display id: range2509036
version: 1
direction: direction/forward
top level: LT
start: 89
end: 126
orientation: inline

CmR
type: SequenceAnnotation
persistent identity: annotation2509051
display id: annotation2509051
version: 1
top level: LT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509051/range2509051/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509051/range2509051/1
type: Range
persistent identity: range2509051
display id: range2509051
version: 1
direction: direction/forward
top level: LT
start: 1140
end: 1799
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2509041
display id: annotation2509041
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509041/range2509041/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509041/range2509041/1
type: Range
persistent identity: range2509041
display id: range2509041
version: 1
direction: direction/forward
top level: LT
start: 209
end: 220
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2509040
display id: annotation2509040
version: 1
top level: LT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509040/range2509040/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509040/range2509040/1
type: Range
persistent identity: range2509040
display id: range2509040
version: 1
direction: direction/forward
top level: LT
start: 173
end: 173
orientation: inline

RGD-His-St
type: ComponentDefinition
persistent identity: BBa K133036
display id: BBa_K133036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RGD-His affinity tag-Stop
creator: Jerneja Mori
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3350
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is His-tag with stop codon and RGD seguence. His-tag is used for detection of His-tagged proteins by anti-His antibodies. RGD is arginine-glycine-aspartic amino acid sequence, present on several extracellular matrix proteins. It is required for binding to integrins.
mutable notes: Cloned into pSB1AK3 vector.
mutable provenance: Part was constructed by primer annealing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K133036_sequence/1

BBa_K823036
type: ComponentDefinition
persistent identity: BBa K823036
display id: BBa_K823036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cMyc-tag (Freiburg standard+RBS)
creator: Jara Radeck
created: 2012-09-06T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: cMyc-tag with RBS in Freiburg standard. prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT This is a part created by the LMU-Munich 2012 team. See here for other usefull parts of the [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks Bacillus Bio B rick B ox].
mutable notes: We designed this part in Freiburg standard for inframe fusions.
mutable provenance: Gene synthesis by gene art.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K823036_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2182255
display id: annotation2182255
version: 1
top level: BBa_K823036
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823036/annotation2182255/range2182255/1

https://synbiohub.org/public/igem/BBa_K823036/annotation2182255/range2182255/1
type: Range
persistent identity: range2182255
display id: range2182255
version: 1
direction: direction/forward
top level: BBa_K823036
start: 1
end: 30
orientation: inline

Met-His
type: ComponentDefinition
persistent identity: BBa K133035
display id: BBa_K133035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Methionine-His affinity tag
creator: Jerneja Mori
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3350
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: His-tag with amino acid Methionine.His-tag is used for detection of His-tagged proteins by anti-His antibodies.
mutable notes: Cloned into pSB1AK3 vector.
mutable provenance: Part was constructed by primer annealing.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K133035_sequence/1

S*Tag
type: ComponentDefinition
persistent identity: BBa J32017
display id: BBa_J32017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-Tag
creator: Austen Heinz
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The S???Tag sequence is a novel fusion peptide tag for recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection in Western blots. Proteins can also be rapidly purified from crude extracts using a one step affinity separation method. The system is based on the strong interaction between the 15aa S???Tag and 103aa S-protein.
mutable notes: .
mutable provenance: Novagen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: S-Tag
sequence: https://synbiohub.org/public/igem/BBa_J32017_sequence/1

S-Tag
type: SequenceAnnotation
persistent identity: annotation1898184
display id: annotation1898184
version: 1
top level: S*Tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J32017/annotation1898184/range1898184/1

https://synbiohub.org/public/igem/BBa_J32017/annotation1898184/range1898184/1
type: Range
persistent identity: range1898184
display id: range1898184
version: 1
direction: direction/forward
top level: S*Tag
start: 1
end: 46
orientation: inline

S-tag
type: ComponentDefinition
persistent identity: BBa T2015
display id: BBa_T2015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-tag tail domain (KETAAAKFERQHMDS)
creator: Reshma Shetty
created: 2009-09-01T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a S-tag tail domain designed to be at the C-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: Included a double TAATAA stop codon.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: S-tag
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_T2015_sequence/1

S-tag
type: SequenceAnnotation
persistent identity: annotation2018265
display id: annotation2018265
version: 1
top level: S-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2015/annotation2018265/range2018265/1

https://synbiohub.org/public/igem/BBa_T2015/annotation2018265/range2018265/1
type: Range
persistent identity: range2018265
display id: range2018265
version: 1
direction: direction/forward
top level: S-tag
start: 1
end: 45
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2018266
display id: annotation2018266
version: 1
top level: S-tag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2015/annotation2018266/range2018266/1

https://synbiohub.org/public/igem/BBa_T2015/annotation2018266/range2018266/1
type: Range
persistent identity: range2018266
display id: range2018266
version: 1
direction: direction/forward
top level: S-tag
start: 46
end: 51
orientation: inline

LigA
type: ComponentDefinition
persistent identity: BBa K525710
display id: BBa_K525710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: LigA1 mit Promotor, RBS, Histag
mutable notes: PCR
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: His-tag
sequence annotation: T7 promoter
sequence annotation: LigA
sequence annotation: RBS
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K525710_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2161302
display id: annotation2161302
version: 1
top level: LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161302/range2161302/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161302/range2161302/1
type: Range
persistent identity: range2161302
display id: range2161302
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 36
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2161304
display id: annotation2161304
version: 1
top level: LigA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161304/range2161304/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161304/range2161304/1
type: Range
persistent identity: range2161304
display id: range2161304
version: 1
direction: direction/forward
top level: LigA
start: 2047
end: 2064
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2161299
display id: annotation2161299
version: 1
top level: LigA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161299/range2161299/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161299/range2161299/1
type: Range
persistent identity: range2161299
display id: range2161299
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 20
orientation: inline

LigA
type: SequenceAnnotation
persistent identity: annotation2161303
display id: annotation2161303
version: 1
top level: LigA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161303/range2161303/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161303/range2161303/1
type: Range
persistent identity: range2161303
display id: range2161303
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 2045
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161301
display id: annotation2161301
version: 1
top level: LigA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161301/range2161301/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161301/range2161301/1
type: Range
persistent identity: range2161301
display id: range2161301
version: 1
direction: direction/forward
top level: LigA
start: 24
end: 27
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161300
display id: annotation2161300
version: 1
top level: LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161300/range2161300/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161300/range2161300/1
type: Range
persistent identity: range2161300
display id: range2161300
version: 1
direction: direction/forward
top level: LigA
start: 21
end: 33
orientation: inline

BBa_K823037
type: ComponentDefinition
persistent identity: BBa K823037
display id: BBa_K823037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 10x His-tag (Freiburg standard+RBS)
creator: Jara Radeck
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: 10xHis-tag with RBS in Freiburg standard. prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT This is a part created by the LMU-Munich 2012 team. We added five tags to the registry, all in the Freiburg standard for N-and C-terminal fusions: 3x Flag tag HA-tag cMyc-tag His-tag Streptavidin-tag Visit our project page for more usefull parts of our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''BacillusB'''''io'''B'''rick'''B'''ox].
mutable notes: We designed the part in the Freiburg standard for N-and C-terminal fusions.
mutable provenance: Gene synthesis by GeneArt.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: His
sequence: https://synbiohub.org/public/igem/BBa_K823037_sequence/1

His
type: SequenceAnnotation
persistent identity: annotation2182324
display id: annotation2182324
version: 1
top level: BBa_K823037
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823037/annotation2182324/range2182324/1

https://synbiohub.org/public/igem/BBa_K823037/annotation2182324/range2182324/1
type: Range
persistent identity: range2182324
display id: range2182324
version: 1
direction: direction/forward
top level: BBa_K823037
start: 7
end: 9
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2182329
display id: annotation2182329
version: 1
top level: BBa_K823037
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823037/annotation2182329/range2182329/1

https://synbiohub.org/public/igem/BBa_K823037/annotation2182329/range2182329/1
type: Range
persistent identity: range2182329
display id: range2182329
version: 1
direction: direction/forward
top level: BBa_K823037
start: 22
end: 24
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2182322
display id: annotation2182322
version: 1
top level: BBa_K823037
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823037/annotation2182322/range2182322/1

https://synbiohub.org/public/igem/BBa_K823037/annotation2182322/range2182322/1
type: Range
persistent identity: range2182322
display id: range2182322
version: 1
direction: direction/forward
top level: BBa_K823037
start: 1
end: 3
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2182323
display id: annotation2182323
version: 1
top level: BBa_K823037
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823037/annotation2182323/range2182323/1

https://synbiohub.org/public/igem/BBa_K823037/annotation2182323/range2182323/1
type: Range
persistent identity: range2182323
display id: range2182323
version: 1
direction: direction/forward
top level: BBa_K823037
start: 4
end: 6
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2182328
display id: annotation2182328
version: 1
top level: BBa_K823037
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823037/annotation2182328/range2182328/1

https://synbiohub.org/public/igem/BBa_K823037/annotation2182328/range2182328/1
type: Range
persistent identity: range2182328
display id: range2182328
version: 1
direction: direction/forward
top level: BBa_K823037
start: 19
end: 21
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2182325
display id: annotation2182325
version: 1
top level: BBa_K823037
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823037/annotation2182325/range2182325/1

https://synbiohub.org/public/igem/BBa_K823037/annotation2182325/range2182325/1
type: Range
persistent identity: range2182325
display id: range2182325
version: 1
direction: direction/forward
top level: BBa_K823037
start: 10
end: 12
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2182330
display id: annotation2182330
version: 1
top level: BBa_K823037
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823037/annotation2182330/range2182330/1

https://synbiohub.org/public/igem/BBa_K823037/annotation2182330/range2182330/1
type: Range
persistent identity: range2182330
display id: range2182330
version: 1
direction: direction/forward
top level: BBa_K823037
start: 25
end: 27
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2182327
display id: annotation2182327
version: 1
top level: BBa_K823037
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823037/annotation2182327/range2182327/1

https://synbiohub.org/public/igem/BBa_K823037/annotation2182327/range2182327/1
type: Range
persistent identity: range2182327
display id: range2182327
version: 1
direction: direction/forward
top level: BBa_K823037
start: 16
end: 18
orientation: inline

His
type: SequenceAnnotation
persistent identity: annotation2182326
display id: annotation2182326
version: 1
top level: BBa_K823037
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823037/annotation2182326/range2182326/1

https://synbiohub.org/public/igem/BBa_K823037/annotation2182326/range2182326/1
type: Range
persistent identity: range2182326
display id: range2182326
version: 1
direction: direction/forward
top level: BBa_K823037
start: 13
end: 15
orientation: inline

HA
type: ComponentDefinition
persistent identity: BBa I712008
display id: BBa_I712008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HA affinity tag
creator: Anja Korenčič
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: HA-tag is used for detection of HA-tagged proteins by anti-HA antibodies.
mutable notes: Gene was designed to be constructed by primer annealing. Includes start codon.
mutable provenance: Part was constructed by primer annealing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_I712008_sequence/1

BBa_K112502
type: ComponentDefinition
persistent identity: BBa K112502
display id: BBa_K112502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < myc > in BBb
creator: Sherine Cheung
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a myc tag without a stop or a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<myc>} basic part K112502 Wobble PCR of cc004/cc005 ( 51 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112502 -------------------------------------------- cc004 Forward Biobricking of {<myc>} CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG cc005 Reverse Biobricking of {<myc>} ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112502_sequence/1

BBa_K112601
type: ComponentDefinition
persistent identity: BBa K112601
display id: BBa_K112601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < AP tag ! in BBb
creator: Sherine Cheung
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is an AP tag with stop codon, but no start. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<AP!} basic part K112601 Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 910) Product is pBca1256-K112601 -------------------------------------------------- SC001 Forward Biobricking of <AP-tag! cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC003 Reverse Biobricking of <AP-tag! ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112601_sequence/1

HA tag
type: ComponentDefinition
persistent identity: BBa T2010
display id: BBa_T2010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hemagglutinin (HA) tag head domain (YPYDVPDYA)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a HA tag head domain designed to be at the N-terminus of a protein. HA tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This domain encodes the HA sequence YPYDVPDYA. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: start codon
sequence annotation: preferred AAA +2 codon
sequence annotation: HA tag
sequence: https://synbiohub.org/public/igem/BBa_T2010_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2017184
display id: annotation2017184
version: 1
top level: HA tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2010/annotation2017184/range2017184/1

https://synbiohub.org/public/igem/BBa_T2010/annotation2017184/range2017184/1
type: Range
persistent identity: range2017184
display id: range2017184
version: 1
direction: direction/forward
top level: HA tag
start: 1
end: 3
orientation: inline

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2017308
display id: annotation2017308
version: 1
top level: HA tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2010/annotation2017308/range2017308/1

https://synbiohub.org/public/igem/BBa_T2010/annotation2017308/range2017308/1
type: Range
persistent identity: range2017308
display id: range2017308
version: 1
direction: direction/forward
top level: HA tag
start: 4
end: 6
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation2017185
display id: annotation2017185
version: 1
top level: HA tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2010/annotation2017185/range2017185/1

https://synbiohub.org/public/igem/BBa_T2010/annotation2017185/range2017185/1
type: Range
persistent identity: range2017185
display id: range2017185
version: 1
direction: direction/forward
top level: HA tag
start: 7
end: 33
orientation: inline

c-Myc
type: ComponentDefinition
persistent identity: BBa T2008
display id: BBa_T2008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c-Myc special internal domain (EQKLISEEDL)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a c-Myc tag special internal domain designed to be within a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This domain encodes the c-Myc sequence EQKLISEEDL. The codons at each amino acid position were selected to avoid rare codons in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: c-Myc tag
sequence: https://synbiohub.org/public/igem/BBa_T2008_sequence/1

c-Myc tag
type: SequenceAnnotation
persistent identity: annotation2017181
display id: annotation2017181
version: 1
top level: c-Myc
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2008/annotation2017181/range2017181/1

https://synbiohub.org/public/igem/BBa_T2008/annotation2017181/range2017181/1
type: Range
persistent identity: range2017181
display id: range2017181
version: 1
direction: direction/forward
top level: c-Myc
start: 1
end: 30
orientation: inline

His
type: ComponentDefinition
persistent identity: BBa K157011
display id: BBa_K157011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His affinity tag; Freiburg standard
creator: Kristian M??ller
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: true
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: His-tag; fusion to proteins facilitates detection, purification, immobilization; NgoMIV / AgeI protein fusion part.
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: His-Tag
sequence: https://synbiohub.org/public/igem/BBa_K157011_sequence/1

His-Tag
type: SequenceAnnotation
persistent identity: annotation2040626
display id: annotation2040626
version: 1
top level: His
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157011/annotation2040626/range2040626/1

https://synbiohub.org/public/igem/BBa_K157011/annotation2040626/range2040626/1
type: Range
persistent identity: range2040626
display id: range2040626
version: 1
direction: direction/forward
top level: His
start: 1
end: 18
orientation: inline

BBa_T2025
type: ComponentDefinition
persistent identity: BBa T2025
display id: BBa_T2025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Glutathione S-transferase internal domain
creator: Reshma Shetty
created: 2009-09-16T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a GST internal domain designed to be between protein domains. GST domains are a type of affinity domain that can be used to purify and/or measure the quantity of proteins.
mutable notes: Rare codons in common model organisms were avoided along with restriction sites used in various assembly standards.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GST
sequence: https://synbiohub.org/public/igem/BBa_T2025_sequence/1

GST
type: SequenceAnnotation
persistent identity: annotation2021346
display id: annotation2021346
version: 1
top level: BBa_T2025
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_T2025/annotation2021346/range2021346/1

https://synbiohub.org/public/igem/BBa_T2025/annotation2021346/range2021346/1
type: Range
persistent identity: range2021346
display id: range2021346
version: 1
direction: direction/forward
top level: BBa_T2025
start: 1
end: 657
orientation: inline

S-tag
type: ComponentDefinition
persistent identity: BBa T2017
display id: BBa_T2017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-tag special internal domain (KETAAAKFERQHMDS)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a S-tag special internal domain designed to between protein domains. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: Rare codons from various model organisms were avoided where possible.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: S-tag
sequence: https://synbiohub.org/public/igem/BBa_T2017_sequence/1

S-tag
type: SequenceAnnotation
persistent identity: annotation2018274
display id: annotation2018274
version: 1
top level: S-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2017/annotation2018274/range2018274/1

https://synbiohub.org/public/igem/BBa_T2017/annotation2018274/range2018274/1
type: Range
persistent identity: range2018274
display id: range2018274
version: 1
direction: direction/forward
top level: S-tag
start: 1
end: 45
orientation: inline

StrepII
type: ComponentDefinition
persistent identity: BBa J18916
display id: BBa_J18916
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strep tag II
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: true
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Protein purification tag. Bound by Strepavidine (optimized: strepTactin(tm)) and eluted with biotin. Allows for mild and efficient elution. SEE ALSO: * novagen.com, gehealthcare.com (former amersham) for purification protocols * BBa_I757014, StrepTag II from Freiburg iGem team, has three codon variations
mutable notes: * gene synthesis * codon optimized for E. coli
mutable provenance: pET52b gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J18916_sequence/1

BBa_K1404007
type: ComponentDefinition
persistent identity: BBa K1404007
display id: BBa_K1404007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His, His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS Paris 2009
sequence annotation: Linker
sequence annotation: Beta sheet 1
sequence annotation: His tag
sequence annotation: CsgG signal
sequence annotation: Beta sheet5
sequence annotation: Beta sheet4
sequence annotation: BBa_K342000
sequence annotation: Sec signal
sequence annotation: Beta sheet2
sequence annotation: GCT into GCC
sequence annotation: CsgA-His1
sequence annotation: Beta sheet3
sequence: https://synbiohub.org/public/igem/BBa_K1404007_sequence/1

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426220
display id: annotation2426220
version: 1
top level: BBa_K1404007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1
type: Range
persistent identity: range2426220
display id: range2426220
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 79
end: 91
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426229
display id: annotation2426229
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1
type: Range
persistent identity: range2426229
display id: range2426229
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 545
end: 556
orientation: inline

Beta sheet 1
type: SequenceAnnotation
persistent identity: annotation2426223
display id: annotation2426223
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1
type: Range
persistent identity: range2426223
display id: range2426223
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 218
end: 286
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426230
display id: annotation2426230
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1
type: Range
persistent identity: range2426230
display id: range2426230
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 557
end: 574
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2426222
display id: annotation2426222
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1
type: Range
persistent identity: range2426222
display id: range2426222
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 152
end: 217
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426228
display id: annotation2426228
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1
type: Range
persistent identity: range2426228
display id: range2426228
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 488
end: 544
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426226
display id: annotation2426226
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1
type: Range
persistent identity: range2426226
display id: range2426226
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 422
end: 487
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426218
display id: annotation2426218
version: 1
top level: BBa_K1404007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1
type: Range
persistent identity: range2426218
display id: range2426218
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 1
end: 70
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2426221
display id: annotation2426221
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1
type: Range
persistent identity: range2426221
display id: range2426221
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 151
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426224
display id: annotation2426224
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1
type: Range
persistent identity: range2426224
display id: range2426224
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 287
end: 352
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426227
display id: annotation2426227
version: 1
top level: BBa_K1404007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1
type: Range
persistent identity: range2426227
display id: range2426227
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 461
end: 463
orientation: inline

CsgA-His1
type: SequenceAnnotation
persistent identity: annotation2426219
display id: annotation2426219
version: 1
top level: BBa_K1404007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1
type: Range
persistent identity: range2426219
display id: range2426219
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 577
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2426225
display id: annotation2426225
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1
type: Range
persistent identity: range2426225
display id: range2426225
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 353
end: 421
orientation: inline

His-Stop
type: ComponentDefinition
persistent identity: BBa K133133
display id: BBa_K133133
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His affinity tag-stop
creator: Katja Kolar
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is His-tag with stop codon. His-tag is used for detection of His-tagged proteins by anti-His antibodies. Cloned into pSB1AK3 vector.
mutable provenance: Part was constructed by primer annealing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K133133_sequence/1

BBa_K112503
type: ComponentDefinition
persistent identity: BBa K112503
display id: BBa_K112503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < myc ! in BBb
creator: Sherine Cheung
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a myc tag without a start, but with a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<myc!} basic part K112503 Wobble PCR of cc004/cc006 ( 54 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI,2227+ 910,L) Product is pBca1256-K112503 -------------------------------------------- cc004 Forward Biobricking of {<myc>} CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG cc006 Reverse Biobricking of {<myc!} ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112503_sequence/1

FLAG
type: ComponentDefinition
persistent identity: BBa T2004
display id: BBa_T2004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FLAG octapeptide head domain (DYKDDDDK)
creator: Reshma Shetty
created: 2008-04-30T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Test of composite parts
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: FLAG octapeptide
sequence annotation: start codon
sequence annotation: preferred AAA +2 codon
sequence: https://synbiohub.org/public/igem/BBa_T2004_sequence/1

FLAG octapeptide
type: SequenceAnnotation
persistent identity: annotation2017175
display id: annotation2017175
version: 1
top level: FLAG
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2004/annotation2017175/range2017175/1

https://synbiohub.org/public/igem/BBa_T2004/annotation2017175/range2017175/1
type: Range
persistent identity: range2017175
display id: range2017175
version: 1
direction: direction/forward
top level: FLAG
start: 7
end: 30
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2017174
display id: annotation2017174
version: 1
top level: FLAG
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2004/annotation2017174/range2017174/1

https://synbiohub.org/public/igem/BBa_T2004/annotation2017174/range2017174/1
type: Range
persistent identity: range2017174
display id: range2017174
version: 1
direction: direction/forward
top level: FLAG
start: 1
end: 3
orientation: inline

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2017306
display id: annotation2017306
version: 1
top level: FLAG
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2004/annotation2017306/range2017306/1

https://synbiohub.org/public/igem/BBa_T2004/annotation2017306/range2017306/1
type: Range
persistent identity: range2017306
display id: range2017306
version: 1
direction: direction/forward
top level: FLAG
start: 4
end: 6
orientation: inline

Strep-tag
type: ComponentDefinition
persistent identity: BBa T2014
display id: BBa_T2014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strep-tag II special internal domain (WSHPQFEK)
creator: Reshma Shetty
created: 2009-08-12T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a Strep tag tail domain designed to be internal to a protein. Strep tags are typically used for detection, purification and immobilization of proteins.
mutable notes: This domain encodes the Strep tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Strep-tag II
sequence: https://synbiohub.org/public/igem/BBa_T2014_sequence/1

Strep-tag II
type: SequenceAnnotation
persistent identity: annotation2017312
display id: annotation2017312
version: 1
top level: Strep-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2014/annotation2017312/range2017312/1

https://synbiohub.org/public/igem/BBa_T2014/annotation2017312/range2017312/1
type: Range
persistent identity: range2017312
display id: range2017312
version: 1
direction: direction/forward
top level: Strep-tag
start: 1
end: 24
orientation: inline

His_FluA
type: ComponentDefinition
persistent identity: BBa S04282
display id: BBa_S04282
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K157011(His affinity tag; Freiburg standard):K157004(Fluorescin-A-binding )
creator: Timo Lorenz
created: 2009-10-04T11:00:00Z
modified: 2015-05-08T01:14:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K157011
component: BBa_K157004
sequence annotation: BBa_K157011
sequence annotation: BBa_K157004
sequence: https://synbiohub.org/public/igem/BBa_S04282_sequence/1

BBa_K157011
type: Component
persistent identity: component2218738
display id: component2218738
version: 1
top level: His_FluA
access: public
definition: His

BBa_K157004
type: Component
persistent identity: component2218740
display id: component2218740
version: 1
top level: His_FluA
access: public
definition: https://synbiohub.org/public/igem/BBa_K157004/1

BBa_K157011
type: SequenceAnnotation
persistent identity: annotation2218738
display id: annotation2218738
version: 1
top level: His_FluA
location: https://synbiohub.org/public/igem/BBa_S04282/annotation2218738/range2218738/1
component: BBa_K157011

https://synbiohub.org/public/igem/BBa_S04282/annotation2218738/range2218738/1
type: Range
persistent identity: range2218738
display id: range2218738
version: 1
direction: direction/reverse
top level: His_FluA
start: 1
end: 18
orientation: inline

BBa_K157004
type: SequenceAnnotation
persistent identity: annotation2218740
display id: annotation2218740
version: 1
top level: His_FluA
location: https://synbiohub.org/public/igem/BBa_S04282/annotation2218740/range2218740/1
component: BBa_K157004

https://synbiohub.org/public/igem/BBa_S04282/annotation2218740/range2218740/1
type: Range
persistent identity: range2218740
display id: range2218740
version: 1
direction: direction/reverse
top level: His_FluA
start: 27
end: 548
orientation: inline

BBa_K1404008
type: ComponentDefinition
persistent identity: BBa K1404008
display id: BBa_K1404008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His*2, double His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: GCT into GCC
sequence annotation: Linker
sequence annotation: His tag
sequence annotation: RBS Paris 2009
sequence annotation: Beta sheet5
sequence annotation: CsgA-His2
sequence annotation: Beta sheet
sequence annotation: BBa_K342000
sequence annotation: CsgD signal
sequence annotation: Beta sheet2
sequence annotation: Beta sheet4
sequence annotation: linker
sequence annotation: SEC signal
sequence: https://synbiohub.org/public/igem/BBa_K1404008_sequence/1

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426516
display id: annotation2426516
version: 1
top level: BBa_K1404008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1
type: Range
persistent identity: range2426516
display id: range2426516
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 461
end: 463
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426518
display id: annotation2426518
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1
type: Range
persistent identity: range2426518
display id: range2426518
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 545
end: 556
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426521
display id: annotation2426521
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1
type: Range
persistent identity: range2426521
display id: range2426521
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 587
end: 604
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426508
display id: annotation2426508
version: 1
top level: BBa_K1404008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1
type: Range
persistent identity: range2426508
display id: range2426508
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 79
end: 91
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426517
display id: annotation2426517
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1
type: Range
persistent identity: range2426517
display id: range2426517
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 488
end: 544
orientation: inline

CsgA-His2
type: SequenceAnnotation
persistent identity: annotation2426510
display id: annotation2426510
version: 1
top level: BBa_K1404008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1
type: Range
persistent identity: range2426510
display id: range2426510
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 607
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426512
display id: annotation2426512
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1
type: Range
persistent identity: range2426512
display id: range2426512
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 218
end: 286
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426514
display id: annotation2426514
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1
type: Range
persistent identity: range2426514
display id: range2426514
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 353
end: 421
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426507
display id: annotation2426507
version: 1
top level: BBa_K1404008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1
type: Range
persistent identity: range2426507
display id: range2426507
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 1
end: 70
orientation: inline

CsgD signal
type: SequenceAnnotation
persistent identity: annotation2426511
display id: annotation2426511
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1
type: Range
persistent identity: range2426511
display id: range2426511
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 152
end: 217
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426513
display id: annotation2426513
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1
type: Range
persistent identity: range2426513
display id: range2426513
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 287
end: 352
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426519
display id: annotation2426519
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1
type: Range
persistent identity: range2426519
display id: range2426519
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 557
end: 574
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426515
display id: annotation2426515
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1
type: Range
persistent identity: range2426515
display id: range2426515
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 422
end: 487
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2426520
display id: annotation2426520
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1
type: Range
persistent identity: range2426520
display id: range2426520
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 575
end: 586
orientation: inline

SEC signal
type: SequenceAnnotation
persistent identity: annotation2426509
display id: annotation2426509
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1
type: Range
persistent identity: range2426509
display id: range2426509
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 151
orientation: inline

Strep-Tag
type: ComponentDefinition
persistent identity: BBa I757014
display id: BBa_I757014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strep-Tag II affinity tag
creator: iGEM Team Freiburg 2007
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: true
experience: experience/None
group u list: _125_
m user id: 0
owner id: 2006
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: * Strep tag as NgoMIV / AgeI protein fusion part * iGEM Team Freiburg 2007
mutable notes: between BioBrick 1.0 sites part is flanked 5' by NgoMIV and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions
mutable provenance: synthetic DNA by GeneArt
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: StrepTagII
sequence: https://synbiohub.org/public/igem/BBa_I757014_sequence/1

StrepTagII
type: SequenceAnnotation
persistent identity: annotation2040664
display id: annotation2040664
version: 1
top level: Strep-Tag
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I757014/annotation2040664/range2040664/1

https://synbiohub.org/public/igem/BBa_I757014/annotation2040664/range2040664/1
type: Range
persistent identity: range2040664
display id: range2040664
version: 1
direction: direction/forward
top level: Strep-Tag
start: 1
end: 24
orientation: inline

BBa_K1319007
type: ComponentDefinition
persistent identity: BBa K1319007
display id: BBa_K1319007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 6xHis tag with double stop codon
creator: Michael Osthege
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a C-terminal 6xHis-tag with a double stop-codon. It can be fused to the C-terminus of proteins to enable purification of fully-translated proteins.
mutable notes: In contrast to other 6x-His tag parts, this part uses the standard double stop codon TAA-TAA.
mutable provenance: The part was designed using the histidin codon CAC and the double stop codons TAA-TAA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: 6xHis
sequence: https://synbiohub.org/public/igem/BBa_K1319007_sequence/1

6xHis
type: SequenceAnnotation
persistent identity: annotation2386538
display id: annotation2386538
version: 1
top level: BBa_K1319007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1319007/annotation2386538/range2386538/1

https://synbiohub.org/public/igem/BBa_K1319007/annotation2386538/range2386538/1
type: Range
persistent identity: range2386538
display id: range2386538
version: 1
direction: direction/forward
top level: BBa_K1319007
start: 1
end: 24
orientation: inline

BBa_K823038
type: ComponentDefinition
persistent identity: BBa K823038
display id: BBa_K823038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strep-tag (Freiburg standard+RBS)
creator: Jara Radeck
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Streptavidin - tag with RBS in Freiburg standard. prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT This is a part created by the LMU-Munich 2012 team. We added five tags to the registry, all in the Freiburg standard for N-and C-terminal fusions: 3x FLAG - tag HA - tag cMyc - tag His - tag Streptavidin - tag Visit our project page for more usefull parts of our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''BacillusB'''''io'''B'''rick'''B'''ox].
mutable notes: We designed the part in Freiburg standard for N- and C-terminal fusions
mutable provenance: Gene synthesis by GeneArt
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Strep
sequence: https://synbiohub.org/public/igem/BBa_K823038_sequence/1

Strep
type: SequenceAnnotation
persistent identity: annotation2182334
display id: annotation2182334
version: 1
top level: BBa_K823038
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K823038/annotation2182334/range2182334/1

https://synbiohub.org/public/igem/BBa_K823038/annotation2182334/range2182334/1
type: Range
persistent identity: range2182334
display id: range2182334
version: 1
direction: direction/forward
top level: BBa_K823038
start: 1
end: 24
orientation: inline

c-Myc
type: ComponentDefinition
persistent identity: BBa T2006
display id: BBa_T2006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c-Myc tail domain (EQKLISEEDL)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a c-Myc tag head domain designed to be at the C-terminus of a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This tail domain encodes the c-Myc sequence EQKLISEEDL. The codons at each amino acid position were selected to avoid rare codons in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: double stop codon
sequence annotation: c-Myc tag
sequence: https://synbiohub.org/public/igem/BBa_T2006_sequence/1

double stop codon
type: SequenceAnnotation
persistent identity: annotation2017178
display id: annotation2017178
version: 1
top level: c-Myc
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2006/annotation2017178/range2017178/1

https://synbiohub.org/public/igem/BBa_T2006/annotation2017178/range2017178/1
type: Range
persistent identity: range2017178
display id: range2017178
version: 1
direction: direction/forward
top level: c-Myc
start: 31
end: 36
orientation: inline

c-Myc tag
type: SequenceAnnotation
persistent identity: annotation2017177
display id: annotation2017177
version: 1
top level: c-Myc
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2006/annotation2017177/range2017177/1

https://synbiohub.org/public/igem/BBa_T2006/annotation2017177/range2017177/1
type: Range
persistent identity: range2017177
display id: range2017177
version: 1
direction: direction/forward
top level: c-Myc
start: 1
end: 30
orientation: inline