This is a S-tag head domain designed to be at the N-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
This head domain begins with Met-Lys to promote efficient translation of the protein in ''E. coli''.
hemagglutinin (HA) tag special internal domain (YPYDVPDYA)
This is a HA tag special internal domain designed to be within a protein. HA tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
This domain encodes the HA sequence YPYDVPDYA. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', C. elegans, ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
hemagglutinin (HA) tag tail domain (YPYDVPDYA)
This is a HA tag tail domain designed to be at the C-terminus of a protein. HA tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
This domain encodes the HA sequence YPYDVPDYA. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
This S-tag can be added between two protein parts. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of S-tag> basic part K112705 Wobble PCR dv013/dv014 (47 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+47, L) Product is pBca1256-K112705 ----------------------------------------------- dv013 Forward Biobricking of S-Tag> cgataGAATTCatgAGATCTatgaaagaaaccgctgctgctaaattcg dv014 Reverse Biobricking of S-Tag> cgttaGGATCCgctgtccatgtgctggcgttcgaatttagcagcagcgg
RFC12 Maltose Binding Protein Internal Domain [malE]
This Maltose-binding protein is designed for either N-terminal or C-terminal fusions. MBP fused proteins can be purified in one step affinity chromatography using a cross-linked amylose matrix and then eluted with 10 mM maltose. Note that the amylose matrix can only be regenerated, in most cases, up to five times. The large size of this tag can also affect the fused protein. MBP fusions often have higher levels of expression and solubility that the native untagged protein, with typical yields of up to 40 mg/liter culture.
pMAL-c2x
This is the polypeptide protein FLAG tag. It is used in antibody binding assays if there is no antibody against the studied protein. It should be put at either the N terminus or the C terminus of the protein This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
Tags are almost always used in conjunction with another protein (the one being studied), so we used the modified Silver BioBrick prefix and suffix to make the construction of funcitonal fusion peptides possible
This sequence is easily recognized by many antibodies.
3x Flag tag with RBS in Freiburg standard. prefix: suffix:
For N- and C-terminal in-frame translational fusion to other genes in Freiburg standard.
This is an HA tag without a start or a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of {<HA>} basic part K112506 Wobble PCR of cc001/cc010 ( 51 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112506 -------------------------------------------- cc001 Forward Biobricking of {<HA>} CgATAgaattcATGagatcttacccatacgacgtcccagac cc010 Reverse biobricking of {<HA>} ccagtggatcccccagcgtagtctgggacgtcgtatggg
c-myc tag is used for detection of proteins by anti-c-myc antibodies.
Gene was designed to be constructed by primer annealing. Includes start codone.
V5 epitope tag tail domain (GKPIPNPLLGLDST)
This is a V5 epitope tag tail domain designed to be at the C-terminus of a protein. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.
A double TAATAA stop codon was included. Rare codons from common model organisms were avoided.
* His-tag * NgoMIV / AgeI protein fusion part * iGEM Team Freiburg 2007
between BioBrick 1.0 sites part is flanked 5' by NgoMIV and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions
This is the polypeptide protein HA tag. It is used in antibody binding assays if there is no antibody against the studied protein. It should be put at either the N terminus or the C terminus of the protein This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
Strep affinity tag II; Freiburg standard
Strep tag as NgoMIV / AgeI protein fusion part, fusion to proteins facilitates detection, purification, immobilization.
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
This codes for six Histidine residues to allow for His column protein purification. It will work best if put at either the N or C terminus of your protein construct. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
GST domain for protein purification. Note: GST is naturally forming dimers.
* aa sequence taken from vector pGEX * codon optimized for E. coli * gene synthesis
This is a FLAG tag with a stop codon, but no start codon. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of {<FLAG!} basic part K112603 Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 910) Product is pBca1256-K112603 ----------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag! cgataGAATTCatgAGATCTgactacaaggatgacgacg SC006 Reverse Biobricking of <FLAG-tag! ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
HA-tag with RBS in Freiburg standard. prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
This is a chitin binding domain (CBD) designed to be at between protein domains. Chitin binding domains are a type of affinity tag that can be used to purify proteins.
Rare codons from common model organisms as well as restriction sites used in various assembly standards were removed.
V5 epitope tag head domain (GKPIPNPLLGLDST)
This is a V5 epitope tag head domain designed to be at the N-terminus of a protein. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.
This head domain begins with Met-Lys to promote efficient translation of the protein in E. coli. Rare codons from various model organisms were avoided where possible.
This is a Strep tag head domain designed to be at the N-terminus of a protein. Strep tags are typically used for detection, purification and immobilization of proteins.
This domain encodes the Strep tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
This is FLAG tag with no start and no stop codon ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of {<FLAG>} basic part K112602 Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 910) Product is pBca1256-K112602 --------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag> cgataGAATTCatgAGATCTgactacaaggatgacgacg SC005 Reverse Biobricking of <FLAG-tag> ccagtGGATCCcttgtcgtcgtcatccttgtagtc
V5 epitope tag special internal domain (GKPIPNPLLGLDST)
This is a V5-tag special internal domain designed to between protein domains. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.
Rare codons from common model organisms were avoided.
This is a c-Myc tag head domain designed to be at the N-terminus of a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
This tail domain encodes the c-Myc sequence EQKLISEEDL. The codons at each amino acid position were selected to avoid rare codons in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts.
A 3 repeat FLAG (DYKDDDDK) antigen epitope tag used for marking proteins of interest. Proteins carrying this tag can be easily detected using commercial western blotting reagents.
This sequence was designed as part of an oligonucleotide primer on each strand then annealed and ligated into the vector.
Synthetic DNA from Oligonucleotide synthesis
FLAG octapeptide special internal domain (DYKDDDDK)
This is a FLAG tag octapeptide special internal domain designed to be internal to the sequence of a protein. FLAG tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
The FLAG sequence AspTyrLysAspAspAspAspLys (DYKDDDDK) is one of the most commonly used FLAG tag sequences. Each of the amino acids in this octapeptide have two possible codon choices. For each amino acid, neither of the two codon choices appear to be rare in the common model organisms. So codons were selected to minimize repeats.
This his tag can be fused to the N terminus of a protein. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of His-Tag> basic part K112703 Mix dv009/dv010 (41bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+41, L) Product is pBca1256-K112703 ----------------------------------------------- dv009 Forward Biobricking of His-Tag> cgataGAATTCatgAGATCTatgCATCATCATCATCATCATGGATCCtaacg dv010 Reverse Biobricking of His-Tag> cgttaGGATCCATGATGATGATGATGATGcatAGATCTcatGAATTCtatgg
VSV-G epitope tag tail domain (YTDIEMNRLGK)
This is a VSV-G epitope tag tail domain designed to be at the C-terminus of a protein. VSV-G tags are a type of epitope tag that can be used to measure the quantity of proteins.
A double TAA TAA stop codon was used. Rare codons from model organisms were avoided where possible.
VSV-G epitope tag special internal domain (YTDIEMNRLGK)
This is a VSV-G epitope tag special internal domain designed to be used between protein domains. VSV-G tags are a type of epitope tag that can be used to measure the quantity of proteins.
Rare codons from model organisms were avoided where possible.
This is a Strep tag tail domain designed to be at the C-terminus of a protein. Strep tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
This domain encodes the Strep tag sequence AWRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
This is a 6x His tag special internal domain is designed to be between protein domains. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
Used both Histidine codons to minimize repeats.
This is an HA tag without a start, but with a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of {<HA!} basic part K112507 Wobble PCR of cc001/cc011 ( 54 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112507 -------------------------------------------- cc001 Forward Biobricking of {<HA>} CgATAgaattcATGagatcttacccatacgacgtcccagac cc011 Reverse biobricking of {<HA!} ccagtggatccttacccagcgtagtctgggacgtcgtatgggtaag
This is a 6x His tag head domain designed to encode the N-terminus of a protein. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
Both Histidine codons were used to minimize repeats. An ATG start codon was used.
Hexahistidine affinity tag. Binds zinc, nickel, or cobalt affinity resins. =Notes= This part is identical to BBa_I757013 (which does not seem to be available though). =References= Wikipedia article: [http://en.wikipedia.org/wiki/Polyhistidine-tag]
Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.
This is a 6x His tag tail domain designed to encode the C-terminus of a protein. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
Used both His codons in order to avoid repeats. Also included a double TAATAA stop codon.
Vector containing Hisx6 and Sortase tag LPETGG
This vector is constructed to simplify the usage of the transpeptidase Sortase A for future iGEM teams. Following a BamHI restriction site we constructed a synthetic sequence consisting of three main elements: 1) A linker (GSSS) to allow for limited mobility of the attached peptides and facilitate correct folding 2) A Sortase A tag (LPETGG) that is used to conjugate the proteins to poly-glycines 3) A standard Hisx6 tag commonly used for Immobilized Metal Affinity Chromatography (IMAC) This sequence follows a BamHI restriction site which can be used by teams to clone in whichever peptide they would like to conjugate using Sortase A.
BamHI is a very convenient restriction enzyme for our application as its restriction site will code for a glycine and a serine, both of which are often used in linkers and tend to be unproblematic in such sequences.
The plasmid was constructed using the iGEM-supplied pSB1C3 and a synthetically produced sequence.
This is His-tag with stop codon and RGD seguence. His-tag is used for detection of His-tagged proteins by anti-His antibodies. RGD is arginine-glycine-aspartic amino acid sequence, present on several extracellular matrix proteins. It is required for binding to integrins.
Part was constructed by primer annealing.
cMyc-tag with RBS in Freiburg standard. prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT This is a part created by the LMU-Munich 2012 team. See here for other usefull parts of the [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks Bacillus Bio B rick B ox].
We designed this part in Freiburg standard for inframe fusions.
His-tag with amino acid Methionine.His-tag is used for detection of His-tagged proteins by anti-His antibodies.
The S???Tag sequence is a novel fusion peptide tag for recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection in Western blots. Proteins can also be rapidly purified from crude extracts using a one step affinity separation method. The system is based on the strong interaction between the 15aa S???Tag and 103aa S-protein.
This is a S-tag tail domain designed to be at the C-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag
10xHis-tag with RBS in Freiburg standard. prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT This is a part created by the LMU-Munich 2012 team. We added five tags to the registry, all in the Freiburg standard for N-and C-terminal fusions: 3x Flag tag HA-tag cMyc-tag His-tag Streptavidin-tag Visit our project page for more usefull parts of our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''BacillusB'''''io'''B'''rick'''B'''ox].
We designed the part in the Freiburg standard for N-and C-terminal fusions.
HA-tag is used for detection of HA-tagged proteins by anti-HA antibodies.
Gene was designed to be constructed by primer annealing. Includes start codon.
This is a myc tag without a stop or a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of {<myc>} basic part K112502 Wobble PCR of cc004/cc005 ( 51 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112502 -------------------------------------------- cc004 Forward Biobricking of {<myc>} CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG cc005 Reverse Biobricking of {<myc>} ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG
This is an AP tag with stop codon, but no start. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of {<AP!} basic part K112601 Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 910) Product is pBca1256-K112601 -------------------------------------------------- SC001 Forward Biobricking of <AP-tag! cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC003 Reverse Biobricking of <AP-tag! ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
hemagglutinin (HA) tag head domain (YPYDVPDYA)
This is a HA tag head domain designed to be at the N-terminus of a protein. HA tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
c-Myc special internal domain (EQKLISEEDL)
This is a c-Myc tag special internal domain designed to be within a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
This domain encodes the c-Myc sequence EQKLISEEDL. The codons at each amino acid position were selected to avoid rare codons in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts.
His-tag; fusion to proteins facilitates detection, purification, immobilization; NgoMIV / AgeI protein fusion part.
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008
Glutathione S-transferase internal domain
This is a GST internal domain designed to be between protein domains. GST domains are a type of affinity domain that can be used to purify and/or measure the quantity of proteins.
Rare codons in common model organisms were avoided along with restriction sites used in various assembly standards.
S-tag special internal domain (KETAAAKFERQHMDS)
This is a S-tag special internal domain designed to between protein domains. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
Rare codons from various model organisms were avoided where possible.
Protein purification tag. Bound by Strepavidine (optimized: strepTactin(tm)) and eluted with biotin. Allows for mild and efficient elution. SEE ALSO: * novagen.com, gehealthcare.com (former amersham) for purification protocols * BBa_I757014, StrepTag II from Freiburg iGem team, has three codon variations
* gene synthesis * codon optimized for E. coli
p70-CsgA-His, His-tagged curli generator
This is His-tag with stop codon. His-tag is used for detection of His-tagged proteins by anti-His antibodies. Cloned into pSB1AK3 vector.
This is a myc tag without a start, but with a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of {<myc!} basic part K112503 Wobble PCR of cc004/cc006 ( 54 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI,2227+ 910,L) Product is pBca1256-K112503 -------------------------------------------- cc004 Forward Biobricking of {<myc>} CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG cc006 Reverse Biobricking of {<myc!} ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC
Strep-tag II special internal domain (WSHPQFEK)
This is a Strep tag tail domain designed to be internal to a protein. Strep tags are typically used for detection, purification and immobilization of proteins.
This domain encodes the Strep tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
K157011(His affinity tag; Freiburg standard):K157004(Fluorescin-A-binding )
p70-CsgA-His*2, double His-tagged curli generator
* Strep tag as NgoMIV / AgeI protein fusion part * iGEM Team Freiburg 2007
This part is a C-terminal 6xHis-tag with a double stop-codon. It can be fused to the C-terminus of proteins to enable purification of fully-translated proteins.
In contrast to other 6x-His tag parts, this part uses the standard double stop codon TAA-TAA.
The part was designed using the histidin codon CAC and the double stop codons TAA-TAA.
Streptavidin - tag with RBS in Freiburg standard. prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT This is a part created by the LMU-Munich 2012 team. We added five tags to the registry, all in the Freiburg standard for N-and C-terminal fusions: 3x FLAG - tag HA - tag cMyc - tag His - tag Streptavidin - tag Visit our project page for more usefull parts of our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''BacillusB'''''io'''B'''rick'''B'''ox].
We designed the part in Freiburg standard for N- and C-terminal fusions
This is a c-Myc tag head domain designed to be at the C-terminus of a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.