//proteindomain/degradation
type: Collection
persistent identity: proteindomain degradation
display id: proteindomain_degradation
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K863010
member: BBa_K1471001
member: BBa_K863012
member: BBa_M0052
member: BBa_K863015
member: BBa_K525001
member: BBa_K863001
member: LAA
member: BBa_K863020
member: hsl1
member: BBa_K525007
member: BBa_K863005
member: AAV
member: cin8
member: BBa_K863011
member: BBa_K525008
member: BBa_K863000
member: BBa_M0051
member: BBa_K863006
member: UmuD
member: cln2
member: BBa_K863021
member: BBa_K525002

UmuD
type: ComponentDefinition
persistent identity: BBa K145012
display id: BBa_K145012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: N-terminal UmuD-derived degradation tag
creator: Benjamien Moeyaert
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:10:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2939
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: UmuD derived N-terminal degradation tag; experimentation demonstrated that residues 1-29 of UmuD are sufficient to impart Lon recognition and degradation.
mutable notes: none
mutable provenance: Derived from the 19 N-terminal amino acids of the UmuD protein. http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=945746&ordinalpos=1&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K145012_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1968219
display id: annotation1968219
version: 1
top level: UmuD
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K145012/annotation1968219/range1968219/1

https://synbiohub.org/public/igem/BBa_K145012/annotation1968219/range1968219/1
type: Range
persistent identity: range1968219
display id: range1968219
version: 1
direction: direction/forward
top level: UmuD
start: 1
end: 3
orientation: inline

cin8
type: ComponentDefinition
persistent identity: BBa K105013
display id: BBa_K105013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cin8 - cell cycle specific degradation tag in yeast
creator: Robin Sorg
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This BioBrick is the xxx domain of the yeast cin8 protein. It has been shown that this region of part of the protein is suffient to get xxx specific degradation via the APC complex.
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: Yeast genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: KEN-box
sequence: https://synbiohub.org/public/igem/BBa_K105013_sequence/1

KEN-box
type: SequenceAnnotation
persistent identity: annotation1983852
display id: annotation1983852
version: 1
top level: cin8
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K105013/annotation1983852/range1983852/1

https://synbiohub.org/public/igem/BBa_K105013/annotation1983852/range1983852/1
type: Range
persistent identity: range1983852
display id: range1983852
version: 1
direction: direction/forward
top level: cin8
start: 63
end: 81
orientation: inline

BBa_K525001
type: ComponentDefinition
persistent identity: BBa K525001
display id: BBa_K525001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SgsE, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SgsE || Red || BisdA || BisdB ; RCU 25
mutable notes: Assembled RCU 25
mutable provenance: BBa_K525303, BBa_K525499, BBa_K123000, BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Freiburg Scar
sequence annotation: BisdB
sequence annotation: BisdA
sequence annotation: Reductase
sequence annotation: AgeI
sequence annotation: B0034
sequence annotation: SgsE domain
sequence annotation: TAA
sequence annotation: K525301
sequence annotation: ATG
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: K525560
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K525001_sequence/1

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160965
display id: annotation2160965
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160965/range2160965/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160965/range2160965/1
type: Range
persistent identity: range2160965
display id: range2160965
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3148
end: 3153
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2160992
display id: annotation2160992
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160992/range2160992/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160992/range2160992/1
type: Range
persistent identity: range2160992
display id: range2160992
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3472
end: 4746
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161002
display id: annotation2161002
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161002/range2161002/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161002/range2161002/1
type: Range
persistent identity: range2161002
display id: range2161002
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3154
end: 3465
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2161003
display id: annotation2161003
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161003/range2161003/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161003/range2161003/1
type: Range
persistent identity: range2161003
display id: range2161003
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2407
end: 3147
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160991
display id: annotation2160991
version: 1
top level: BBa_K525001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160991/range2160991/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160991/range2160991/1
type: Range
persistent identity: range2160991
display id: range2160991
version: 1
direction: direction/forward
top level: BBa_K525001
start: 4738
end: 4743
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160959
display id: annotation2160959
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160959/range2160959/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160959/range2160959/1
type: Range
persistent identity: range2160959
display id: range2160959
version: 1
direction: direction/forward
top level: BBa_K525001
start: 53
end: 64
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2161004
display id: annotation2161004
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161004/range2161004/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161004/range2161004/1
type: Range
persistent identity: range2161004
display id: range2161004
version: 1
direction: direction/forward
top level: BBa_K525001
start: 76
end: 2400
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160990
display id: annotation2160990
version: 1
top level: BBa_K525001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160990/range2160990/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160990/range2160990/1
type: Range
persistent identity: range2160990
display id: range2160990
version: 1
direction: direction/forward
top level: BBa_K525001
start: 4744
end: 4746
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2160962
display id: annotation2160962
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160962/range2160962/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160962/range2160962/1
type: Range
persistent identity: range2160962
display id: range2160962
version: 1
direction: direction/forward
top level: BBa_K525001
start: 73
end: 2400
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160961
display id: annotation2160961
version: 1
top level: BBa_K525001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160961/range2160961/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160961/range2160961/1
type: Range
persistent identity: range2160961
display id: range2160961
version: 1
direction: direction/forward
top level: BBa_K525001
start: 73
end: 75
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160958
display id: annotation2160958
version: 1
top level: BBa_K525001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160958/range2160958/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160958/range2160958/1
type: Range
persistent identity: range2160958
display id: range2160958
version: 1
direction: direction/forward
top level: BBa_K525001
start: 20
end: 44
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160963
display id: annotation2160963
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160963/range2160963/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160963/range2160963/1
type: Range
persistent identity: range2160963
display id: range2160963
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2401
end: 2406
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2160957
display id: annotation2160957
version: 1
top level: BBa_K525001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160957/range2160957/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160957/range2160957/1
type: Range
persistent identity: range2160957
display id: range2160957
version: 1
direction: direction/forward
top level: BBa_K525001
start: 1
end: 20
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160989
display id: annotation2160989
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160989/range2160989/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160989/range2160989/1
type: Range
persistent identity: range2160989
display id: range2160989
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3466
end: 3471
orientation: inline

K525560
type: SequenceAnnotation
persistent identity: annotation2160964
display id: annotation2160964
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160964/range2160964/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160964/range2160964/1
type: Range
persistent identity: range2160964
display id: range2160964
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2407
end: 4746
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2160960
display id: annotation2160960
version: 1
top level: BBa_K525001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160960/range2160960/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160960/range2160960/1
type: Range
persistent identity: range2160960
display id: range2160960
version: 1
direction: direction/forward
top level: BBa_K525001
start: 56
end: 59
orientation: inline

BBa_K525007
type: ComponentDefinition
persistent identity: BBa K525007
display id: BBa_K525007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of S-Layer SgsE with T7/lac and BisdA and BisdB
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PT7 + RBS + SgsE || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525303, BBa_K123000, BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Freiburg Scar
sequence annotation: K525301
sequence annotation: BisdA
sequence annotation: RBS
sequence annotation: B0034
sequence annotation: T7
sequence annotation: K525515
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence annotation: ATG
sequence annotation: lac operator
sequence annotation: BisdB
sequence annotation: SgsE domain
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K525007_sequence/1

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161170
display id: annotation2161170
version: 1
top level: BBa_K525007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161170/range2161170/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161170/range2161170/1
type: Range
persistent identity: range2161170
display id: range2161170
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2401
end: 2406
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2161168
display id: annotation2161168
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161168/range2161168/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161168/range2161168/1
type: Range
persistent identity: range2161168
display id: range2161168
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 2400
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161172
display id: annotation2161172
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161172/range2161172/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161172/range2161172/1
type: Range
persistent identity: range2161172
display id: range2161172
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2407
end: 2718
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161165
display id: annotation2161165
version: 1
top level: BBa_K525007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161165/range2161165/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161165/range2161165/1
type: Range
persistent identity: range2161165
display id: range2161165
version: 1
direction: direction/forward
top level: BBa_K525007
start: 56
end: 59
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161164
display id: annotation2161164
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161164/range2161164/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161164/range2161164/1
type: Range
persistent identity: range2161164
display id: range2161164
version: 1
direction: direction/forward
top level: BBa_K525007
start: 53
end: 64
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161173
display id: annotation2161173
version: 1
top level: BBa_K525007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161173/range2161173/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161173/range2161173/1
type: Range
persistent identity: range2161173
display id: range2161173
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2719
end: 2724
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2161162
display id: annotation2161162
version: 1
top level: BBa_K525007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161162/range2161162/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161162/range2161162/1
type: Range
persistent identity: range2161162
display id: range2161162
version: 1
direction: direction/forward
top level: BBa_K525007
start: 1
end: 20
orientation: inline

K525515
type: SequenceAnnotation
persistent identity: annotation2161171
display id: annotation2161171
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161171/range2161171/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161171/range2161171/1
type: Range
persistent identity: range2161171
display id: range2161171
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2407
end: 3999
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161175
display id: annotation2161175
version: 1
top level: BBa_K525007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161175/range2161175/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161175/range2161175/1
type: Range
persistent identity: range2161175
display id: range2161175
version: 1
direction: direction/forward
top level: BBa_K525007
start: 3991
end: 3996
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161169
display id: annotation2161169
version: 1
top level: BBa_K525007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161169/range2161169/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161169/range2161169/1
type: Range
persistent identity: range2161169
display id: range2161169
version: 1
direction: direction/forward
top level: BBa_K525007
start: 76
end: 82
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161166
display id: annotation2161166
version: 1
top level: BBa_K525007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161166/range2161166/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161166/range2161166/1
type: Range
persistent identity: range2161166
display id: range2161166
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 75
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2161163
display id: annotation2161163
version: 1
top level: BBa_K525007
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161163/range2161163/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161163/range2161163/1
type: Range
persistent identity: range2161163
display id: range2161163
version: 1
direction: direction/forward
top level: BBa_K525007
start: 20
end: 44
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2161174
display id: annotation2161174
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161174/range2161174/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161174/range2161174/1
type: Range
persistent identity: range2161174
display id: range2161174
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2725
end: 3999
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2161167
display id: annotation2161167
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161167/range2161167/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161167/range2161167/1
type: Range
persistent identity: range2161167
display id: range2161167
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 2400
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161176
display id: annotation2161176
version: 1
top level: BBa_K525007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161176/range2161176/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161176/range2161176/1
type: Range
persistent identity: range2161176
display id: range2161176
version: 1
direction: direction/forward
top level: BBa_K525007
start: 3997
end: 3999
orientation: inline

hsl1
type: ComponentDefinition
persistent identity: BBa K105015
display id: BBa_K105015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hsl1 - cell cycle dependent degradation tag in yeast
creator: Robin Sorg
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This BioBrick is a fragement of the yeast hsl1 protein. Fused to an artificial transcription factor it tiggers the degradation in??? phase via the ???.
mutable notes: To enable the fusion with the protein in frame the vector of this BioBrick has no base pair in between the restriction sites and the BioBrick. Furthermore, this coding sequence does not include a start codon. For more information about this issus, see: Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: Yeast genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K105015_sequence/1

BBa_K863011
type: ComponentDefinition
persistent identity: BBa K863011
display id: BBa_K863011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from Thermus thermophilus
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tthl (Laccase from Thermus thermophilus) coding sequence
mutable notes: test
mutable provenance: Thermus thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAG
sequence annotation: tthl
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863011_sequence/1

TAG
type: SequenceAnnotation
persistent identity: annotation2184889
display id: annotation2184889
version: 1
top level: BBa_K863011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184889/range2184889/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184889/range2184889/1
type: Range
persistent identity: range2184889
display id: range2184889
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1387
end: 1389
orientation: inline

tthl
type: SequenceAnnotation
persistent identity: annotation2184888
display id: annotation2184888
version: 1
top level: BBa_K863011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184888/range2184888/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184888/range2184888/1
type: Range
persistent identity: range2184888
display id: range2184888
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1
end: 1389
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184806
display id: annotation2184806
version: 1
top level: BBa_K863011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184806/range2184806/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184806/range2184806/1
type: Range
persistent identity: range2184806
display id: range2184806
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1
end: 3
orientation: inline

BBa_K863021
type: ComponentDefinition
persistent identity: BBa K863021
display id: BBa_K863021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bhal laccase from Bacillus halodurans
creator: Isabel Huber
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bhal laccase from Bacillus halodurans
mutable notes: test
mutable provenance: Bacillus halodurans C-125
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: CDS
sequence: https://synbiohub.org/public/igem/BBa_K863021_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2213231
display id: annotation2213231
version: 1
top level: BBa_K863021
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213231/range2213231/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213231/range2213231/1
type: Range
persistent identity: range2213231
display id: range2213231
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1501
end: 1503
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2213230
display id: annotation2213230
version: 1
top level: BBa_K863021
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213230/range2213230/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213230/range2213230/1
type: Range
persistent identity: range2213230
display id: range2213230
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1
end: 3
orientation: inline

CDS
type: SequenceAnnotation
persistent identity: annotation2213229
display id: annotation2213229
version: 1
top level: BBa_K863021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213229/range2213229/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213229/range2213229/1
type: Range
persistent identity: range2213229
display id: range2213229
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1
end: 1503
orientation: inline

BBa_K1471001
type: ComponentDefinition
persistent identity: BBa K1471001
display id: BBa_K1471001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MerB.
creator: Jaime Antonio del Castillo Nu??ez
created: 2014-10-07T11:00:00Z
modified: 2015-07-22T02:40:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1850_
m user id: 4206
owner id: 16614
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The MerB gene codes for the proetin which function is to cleave the organic part of the molecule of methyl-mercury
mutable notes: efwgeg
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MerB
sequence: https://synbiohub.org/public/igem/BBa_K1471001_sequence/1

MerB
type: SequenceAnnotation
persistent identity: annotation2419568
display id: annotation2419568
version: 1
top level: BBa_K1471001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1471001/annotation2419568/range2419568/1

https://synbiohub.org/public/igem/BBa_K1471001/annotation2419568/range2419568/1
type: Range
persistent identity: range2419568
display id: range2419568
version: 1
direction: direction/forward
top level: BBa_K1471001
start: 1
end: 639
orientation: inline

BBa_K863020
type: ComponentDefinition
persistent identity: BBa K863020
display id: BBa_K863020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bhal laccase from Bacillus halodurans with T7 and HIS
mutable notes: test
mutable provenance: Bacillus halodurans C-125
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6 x HIS
sequence annotation: TAA
sequence annotation: RBS
sequence annotation: bhal
sequence annotation: T7
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863020_sequence/1

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2202255
display id: annotation2202255
version: 1
top level: BBa_K863020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202255/range2202255/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202255/range2202255/1
type: Range
persistent identity: range2202255
display id: range2202255
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1534
end: 1551
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2202257
display id: annotation2202257
version: 1
top level: BBa_K863020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202257/range2202257/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202257/range2202257/1
type: Range
persistent identity: range2202257
display id: range2202257
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1555
end: 1557
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2202252
display id: annotation2202252
version: 1
top level: BBa_K863020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202252/range2202252/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202252/range2202252/1
type: Range
persistent identity: range2202252
display id: range2202252
version: 1
direction: direction/forward
top level: BBa_K863020
start: 25
end: 30
orientation: inline

bhal
type: SequenceAnnotation
persistent identity: annotation2202254
display id: annotation2202254
version: 1
top level: BBa_K863020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202254/range2202254/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202254/range2202254/1
type: Range
persistent identity: range2202254
display id: range2202254
version: 1
direction: direction/forward
top level: BBa_K863020
start: 34
end: 1533
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2202256
display id: annotation2202256
version: 1
top level: BBa_K863020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202256/range2202256/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202256/range2202256/1
type: Range
persistent identity: range2202256
display id: range2202256
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1552
end: 1554
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2202251
display id: annotation2202251
version: 1
top level: BBa_K863020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202251/range2202251/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202251/range2202251/1
type: Range
persistent identity: range2202251
display id: range2202251
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1
end: 23
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2202253
display id: annotation2202253
version: 1
top level: BBa_K863020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202253/range2202253/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202253/range2202253/1
type: Range
persistent identity: range2202253
display id: range2202253
version: 1
direction: direction/forward
top level: BBa_K863020
start: 34
end: 36
orientation: inline

BBa_K525002
type: ComponentDefinition
persistent identity: BBa K525002
display id: BBa_K525002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SbpA || Red || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525403; BBa_K525499; BBa_K123000 ; BBa_K123001 ; RCU 25
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: K525560
sequence annotation: Freiburg sacr
sequence annotation: SbpA domain
sequence annotation: Freiburg scar
sequence annotation: B0034
sequence annotation: AgeI
sequence annotation: lac operator
sequence annotation: TAA
sequence annotation: BisdA
sequence annotation: RBS
sequence annotation: NgoMIV
sequence annotation: Reductase
sequence annotation: T7 promoter
sequence annotation: BisdB
sequence annotation: K525401
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K525002_sequence/1

K525560
type: SequenceAnnotation
persistent identity: annotation2160929
display id: annotation2160929
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160929/range2160929/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160929/range2160929/1
type: Range
persistent identity: range2160929
display id: range2160929
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3199
end: 5538
orientation: inline

Freiburg sacr
type: SequenceAnnotation
persistent identity: annotation2160932
display id: annotation2160932
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160932/range2160932/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160932/range2160932/1
type: Range
persistent identity: range2160932
display id: range2160932
version: 1
direction: direction/forward
top level: BBa_K525002
start: 4258
end: 4262
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2160925
display id: annotation2160925
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160925/range2160925/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160925/range2160925/1
type: Range
persistent identity: range2160925
display id: range2160925
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 3192
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160927
display id: annotation2160927
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160927/range2160927/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160927/range2160927/1
type: Range
persistent identity: range2160927
display id: range2160927
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3193
end: 3198
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161005
display id: annotation2161005
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2161005/range2161005/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2161005/range2161005/1
type: Range
persistent identity: range2161005
display id: range2161005
version: 1
direction: direction/forward
top level: BBa_K525002
start: 53
end: 64
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160934
display id: annotation2160934
version: 1
top level: BBa_K525002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160934/range2160934/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160934/range2160934/1
type: Range
persistent identity: range2160934
display id: range2160934
version: 1
direction: direction/forward
top level: BBa_K525002
start: 5530
end: 5535
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160922
display id: annotation2160922
version: 1
top level: BBa_K525002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160922/range2160922/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160922/range2160922/1
type: Range
persistent identity: range2160922
display id: range2160922
version: 1
direction: direction/forward
top level: BBa_K525002
start: 20
end: 40
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160935
display id: annotation2160935
version: 1
top level: BBa_K525002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160935/range2160935/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160935/range2160935/1
type: Range
persistent identity: range2160935
display id: range2160935
version: 1
direction: direction/forward
top level: BBa_K525002
start: 5536
end: 5538
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160930
display id: annotation2160930
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160930/range2160930/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160930/range2160930/1
type: Range
persistent identity: range2160930
display id: range2160930
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3940
end: 3945
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2160931
display id: annotation2160931
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160931/range2160931/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160931/range2160931/1
type: Range
persistent identity: range2160931
display id: range2160931
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3946
end: 4258
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2160923
display id: annotation2160923
version: 1
top level: BBa_K525002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160923/range2160923/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160923/range2160923/1
type: Range
persistent identity: range2160923
display id: range2160923
version: 1
direction: direction/forward
top level: BBa_K525002
start: 56
end: 59
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160926
display id: annotation2160926
version: 1
top level: BBa_K525002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160926/range2160926/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160926/range2160926/1
type: Range
persistent identity: range2160926
display id: range2160926
version: 1
direction: direction/forward
top level: BBa_K525002
start: 76
end: 81
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2160928
display id: annotation2160928
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160928/range2160928/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160928/range2160928/1
type: Range
persistent identity: range2160928
display id: range2160928
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3199
end: 3939
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2160920
display id: annotation2160920
version: 1
top level: BBa_K525002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160920/range2160920/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160920/range2160920/1
type: Range
persistent identity: range2160920
display id: range2160920
version: 1
direction: direction/forward
top level: BBa_K525002
start: 1
end: 20
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2160933
display id: annotation2160933
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160933/range2160933/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160933/range2160933/1
type: Range
persistent identity: range2160933
display id: range2160933
version: 1
direction: direction/forward
top level: BBa_K525002
start: 4263
end: 5538
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2160921
display id: annotation2160921
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160921/range2160921/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160921/range2160921/1
type: Range
persistent identity: range2160921
display id: range2160921
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 3192
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160924
display id: annotation2160924
version: 1
top level: BBa_K525002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160924/range2160924/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160924/range2160924/1
type: Range
persistent identity: range2160924
display id: range2160924
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 75
orientation: inline

BBa_K863001
type: ComponentDefinition
persistent identity: BBa K863001
display id: BBa_K863001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bpul laccase from Bacillus pumilus
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bpul (Laccase from Bacillus pumilus)
mutable notes: test
mutable provenance: Bacillus pumilus DSM 27 (ATCC7061)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: A (Original) - T (Mutation)
sequence annotation: bpul
sequence annotation: ATG
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863001_sequence/1

A (Original) - T (Mutation)
type: SequenceAnnotation
persistent identity: annotation2184905
display id: annotation2184905
version: 1
top level: BBa_K863001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184905/range2184905/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184905/range2184905/1
type: Range
persistent identity: range2184905
display id: range2184905
version: 1
direction: direction/forward
top level: BBa_K863001
start: 801
end: 801
orientation: inline

bpul
type: SequenceAnnotation
persistent identity: annotation2184621
display id: annotation2184621
version: 1
top level: BBa_K863001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184621/range2184621/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184621/range2184621/1
type: Range
persistent identity: range2184621
display id: range2184621
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1
end: 1533
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184622
display id: annotation2184622
version: 1
top level: BBa_K863001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184622/range2184622/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184622/range2184622/1
type: Range
persistent identity: range2184622
display id: range2184622
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1
end: 3
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184641
display id: annotation2184641
version: 1
top level: BBa_K863001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184641/range2184641/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184641/range2184641/1
type: Range
persistent identity: range2184641
display id: range2184641
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1531
end: 1533
orientation: inline

BBa_K863015
type: ComponentDefinition
persistent identity: BBa K863015
display id: BBa_K863015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xccl laccase from Xanthomonas campestris with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: xccl laccase from Xanthomonas campestris with pT7 and HIS tag
mutable notes: test
mutable provenance: Xanthomonas campestris pv. campestris B100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: xccl
sequence annotation: 6x HIS
sequence annotation: ATG
sequence annotation: T7
sequence: https://synbiohub.org/public/igem/BBa_K863015_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2198674
display id: annotation2198674
version: 1
top level: BBa_K863015
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198674/range2198674/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198674/range2198674/1
type: Range
persistent identity: range2198674
display id: range2198674
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1861
end: 1863
orientation: inline

xccl
type: SequenceAnnotation
persistent identity: annotation2198673
display id: annotation2198673
version: 1
top level: BBa_K863015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198673/range2198673/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198673/range2198673/1
type: Range
persistent identity: range2198673
display id: range2198673
version: 1
direction: direction/forward
top level: BBa_K863015
start: 34
end: 1837
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2198697
display id: annotation2198697
version: 1
top level: BBa_K863015
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198697/range2198697/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198697/range2198697/1
type: Range
persistent identity: range2198697
display id: range2198697
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1858
end: 1860
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2198699
display id: annotation2198699
version: 1
top level: BBa_K863015
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198699/range2198699/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198699/range2198699/1
type: Range
persistent identity: range2198699
display id: range2198699
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1838
end: 1857
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2198671
display id: annotation2198671
version: 1
top level: BBa_K863015
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198671/range2198671/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198671/range2198671/1
type: Range
persistent identity: range2198671
display id: range2198671
version: 1
direction: direction/forward
top level: BBa_K863015
start: 34
end: 36
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2198670
display id: annotation2198670
version: 1
top level: BBa_K863015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198670/range2198670/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198670/range2198670/1
type: Range
persistent identity: range2198670
display id: range2198670
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1
end: 23
orientation: inline

BBa_K863010
type: ComponentDefinition
persistent identity: BBa K863010
display id: BBa_K863010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag
mutable notes: test
mutable provenance: Thermus thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tthl
sequence annotation: TAA
sequence annotation: 6x HIS
sequence annotation: T7 promoter
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863010_sequence/1

tthl
type: SequenceAnnotation
persistent identity: annotation2184732
display id: annotation2184732
version: 1
top level: BBa_K863010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184732/range2184732/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184732/range2184732/1
type: Range
persistent identity: range2184732
display id: range2184732
version: 1
direction: direction/forward
top level: BBa_K863010
start: 34
end: 1419
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184782
display id: annotation2184782
version: 1
top level: BBa_K863010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184782/range2184782/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184782/range2184782/1
type: Range
persistent identity: range2184782
display id: range2184782
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1438
end: 1440
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2184733
display id: annotation2184733
version: 1
top level: BBa_K863010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184733/range2184733/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184733/range2184733/1
type: Range
persistent identity: range2184733
display id: range2184733
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1420
end: 1437
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184783
display id: annotation2184783
version: 1
top level: BBa_K863010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184783/range2184783/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184783/range2184783/1
type: Range
persistent identity: range2184783
display id: range2184783
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1441
end: 1443
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2184708
display id: annotation2184708
version: 1
top level: BBa_K863010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184708/range2184708/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184708/range2184708/1
type: Range
persistent identity: range2184708
display id: range2184708
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1
end: 23
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184671
display id: annotation2184671
version: 1
top level: BBa_K863010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184671/range2184671/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184671/range2184671/1
type: Range
persistent identity: range2184671
display id: range2184671
version: 1
direction: direction/forward
top level: BBa_K863010
start: 34
end: 36
orientation: inline

BBa_K863000
type: ComponentDefinition
persistent identity: BBa K863000
display id: BBa_K863000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: bpul (Laccase from Bacillus pumilus) with T7, RBS and HIS tag
mutable notes: test
mutable provenance: Bacillus pumilus DSM 27 (ATCC7061)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6 x HIS
sequence annotation: A (Original) T (Mutation)
sequence annotation: TAA
sequence annotation: bpul
sequence annotation: RBS
sequence annotation: T7- Promotor BBa_I719005
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863000_sequence/1

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2184913
display id: annotation2184913
version: 1
top level: BBa_K863000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184913/range2184913/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184913/range2184913/1
type: Range
persistent identity: range2184913
display id: range2184913
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1557
end: 1581
orientation: inline

A (Original) T (Mutation)
type: SequenceAnnotation
persistent identity: annotation2184912
display id: annotation2184912
version: 1
top level: BBa_K863000
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184912/range2184912/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184912/range2184912/1
type: Range
persistent identity: range2184912
display id: range2184912
version: 1
direction: direction/forward
top level: BBa_K863000
start: 834
end: 834
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184914
display id: annotation2184914
version: 1
top level: BBa_K863000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184914/range2184914/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184914/range2184914/1
type: Range
persistent identity: range2184914
display id: range2184914
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1582
end: 1584
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184915
display id: annotation2184915
version: 1
top level: BBa_K863000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184915/range2184915/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184915/range2184915/1
type: Range
persistent identity: range2184915
display id: range2184915
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1585
end: 1587
orientation: inline

bpul
type: SequenceAnnotation
persistent identity: annotation2184911
display id: annotation2184911
version: 1
top level: BBa_K863000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184911/range2184911/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184911/range2184911/1
type: Range
persistent identity: range2184911
display id: range2184911
version: 1
direction: direction/forward
top level: BBa_K863000
start: 34
end: 1556
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2184909
display id: annotation2184909
version: 1
top level: BBa_K863000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184909/range2184909/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184909/range2184909/1
type: Range
persistent identity: range2184909
display id: range2184909
version: 1
direction: direction/forward
top level: BBa_K863000
start: 25
end: 30
orientation: inline

T7- Promotor BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2184908
display id: annotation2184908
version: 1
top level: BBa_K863000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184908/range2184908/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184908/range2184908/1
type: Range
persistent identity: range2184908
display id: range2184908
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1
end: 23
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184910
display id: annotation2184910
version: 1
top level: BBa_K863000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184910/range2184910/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184910/range2184910/1
type: Range
persistent identity: range2184910
display id: range2184910
version: 1
direction: direction/forward
top level: BBa_K863000
start: 34
end: 36
orientation: inline

LAA
type: ComponentDefinition
persistent identity: BBa M0050
display id: BBa_M0050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AANDENYALAA. (Very fast) SsrA degradation tag.
creator: Felix Moser
created: 2007-12-05T12:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2398
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This sequence codes for the amino acid sequence AANDENYALAA, which when fused to the C-terminal of proteins, will make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease. The following rates of degradation of this tag are pulled from the corresponding references below: ~5 Vmax/ [Clpx6] min-1 from (1) ~0.5%/min on log scale from (2) ~1 min half life from (3) See the following references for further information on degradation rates and mechanisms of this tag: (1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. (2)Flynn et al 2003. Mol. Cell. 11: 671. Flynn et al. 2001. PNAS 98(19): 10584. Anderson et al 1998. App. Env. Microbiol. 64(6):2240.
mutable notes: C-terminal tag. Degradation rate is very fast. Deviations from this sequence in key amino acids will lower degradation rates (see Parts BBa_M0051, BBa_M0052, BBa_M0053). Three C-terminal aa's (LAA in this case) are necessary and sufficient for ClpX binding and degradation. Upstream aa sequence serves as a binding site for SspB, which guides rapid binding to ClpX.
mutable provenance: C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: WT SsrA tag AANDENYALAA
sequence: https://synbiohub.org/public/igem/BBa_M0050_sequence/1

WT SsrA tag AANDENYALAA
type: SequenceAnnotation
persistent identity: annotation1958880
display id: annotation1958880
version: 1
top level: LAA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_M0050/annotation1958880/range1958880/1

https://synbiohub.org/public/igem/BBa_M0050/annotation1958880/range1958880/1
type: Range
persistent identity: range1958880
display id: range1958880
version: 1
direction: direction/forward
top level: LAA
start: 1
end: 33
orientation: inline

BBa_M0052
type: ComponentDefinition
persistent identity: BBa M0052
display id: BBa_M0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AANDENYADAS (moderately fast) SsrA degradation tag.
creator: Felix Moser
created: 2007-12-05T12:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2398
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This sequence codes for the amino acid sequence AANDENYADAS, a slower variation of the WT SsrA tag sequence, which, when fused to the C-terminal of proteins, will make the protein susceptible to moderately fast degradation through SspB-mediated binding to the ClpX protease. The following rates of degradation of this tag are pulled from the corresponding references below: ~1 Vmax/ [Clpx6] min-1 from (1). See the following references for further information on degradation rates and mechanisms of this tag: (1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. (2)Flynn et al 2003. Mol. Cell. 11: 671. Flynn et al. 2001. PNAS 98(19): 10584. Anderson et al 1998. App. Env. Microbiol. 64(6):2240.
mutable notes: C-terminal tag. Degradation rate is moderately fast. Variations of this sequence yield different degradation rates(see Parts BBa_M0050, BBa_M0051, BBa_M0053). Sequence derived from reverse translation of AANDENYADAS sequence using codon usage optimized for E. coli. Three C-terminal aa's (DAS in this case) are necessary and sufficient for ClpX binding and degradation. Upstream aa sequence serves as a binding site for SspB, which guides rapid binding to ClpX. See sources on the main part page for further information about the mechanism of the system. NOTE: this sequence has only the amino acid sequence for the tag and bears NO STOP CODONS, so be sure to include them if you use this sequence.
mutable provenance: This variation of the SsrA tag was studied by McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. SsrA tags are prevalent in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: AANDENYADAS SsrA degradation tag
sequence: https://synbiohub.org/public/igem/BBa_M0052_sequence/1

AANDENYADAS SsrA degradation tag
type: SequenceAnnotation
persistent identity: annotation1958882
display id: annotation1958882
version: 1
top level: BBa_M0052
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_M0052/annotation1958882/range1958882/1

https://synbiohub.org/public/igem/BBa_M0052/annotation1958882/range1958882/1
type: Range
persistent identity: range1958882
display id: range1958882
version: 1
direction: direction/forward
top level: BBa_M0052
start: 1
end: 33
orientation: inline

cln2
type: ComponentDefinition
persistent identity: BBa K105014
display id: BBa_K105014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cln2 PEST destabilization domain for rapid protein turnover
creator: Robin Sorg
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick is the xxx domain of the yeast cln2 protein. It has been shown that this region of part of the protein is suffient to get xxx specific degradation via the APC complex. <br\n>
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: Yeast genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K105014_sequence/1

BBa_M0051
type: ComponentDefinition
persistent identity: BBa M0051
display id: BBa_M0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AANDENYNYADAS. (Fast) SsrA degradation tag.
creator: Felix Moser
created: 2007-12-05T12:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2398
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This sequence codes for the amino acid sequence AANDENYNYADAS, a variation of the WT SsrA tag sequence, which, when fused to the C-terminal of proteins, will make the protein susceptible to fast degradation through SspB-mediated binding to the ClpX protease. The following rates of degradation of this tag are pulled from the corresponding references below: ~2 Vmax/ [Clpx6] min-1 from (1). See the following references for further information on degradation rates and mechanisms of this tag: (1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. (2)Flynn et al 2003. Mol. Cell. 11: 671. Flynn et al. 2001. PNAS 98(19): 10584. Anderson et al 1998. App. Env. Microbiol. 64(6):2240.
mutable notes: C-terminal tag. Degradation rate is fast. Variations of this sequence yield different degradation rates(see Parts BBa_M0050, BBa_M0052, BBa_M0053). Sequence derived from reverse translation of AANDENYNYADAS sequence using codon usage optimized for E. coli. Three C-terminal aa's (DAS in this case) are necessary and sufficient for ClpX binding and degradation. Upstream aa sequence serves as a binding site for SspB, which guides rapid binding to ClpX. See sources on the main part page for further information about the mechanism of the system. NOTE: this sequence has only the amino acid sequence for the tag and bears NO STOP CODONS, so be sure to inlclude them if you use this sequence.
mutable provenance: This variation of the SsrA tag was studied by McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: AANDENYNYADAS SsrA deg. tag
sequence: https://synbiohub.org/public/igem/BBa_M0051_sequence/1

AANDENYNYADAS SsrA deg. tag
type: SequenceAnnotation
persistent identity: annotation1958881
display id: annotation1958881
version: 1
top level: BBa_M0051
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_M0051/annotation1958881/range1958881/1

https://synbiohub.org/public/igem/BBa_M0051/annotation1958881/range1958881/1
type: Range
persistent identity: range1958881
display id: range1958881
version: 1
direction: direction/forward
top level: BBa_M0051
start: 1
end: 39
orientation: inline

BBa_K863012
type: ComponentDefinition
persistent identity: BBa K863012
display id: BBa_K863012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: tthl laccase (T. thermophilus) with constitutive promoter and HIS tag
mutable notes: test
mutable provenance: T. thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23100
sequence annotation: BBa_J61101
sequence annotation: ATG
sequence annotation: g (original) --> a (mutation)
sequence annotation: TAA
sequence annotation: 6 x HIS
sequence annotation: tthl
sequence: https://synbiohub.org/public/igem/BBa_K863012_sequence/1

J23100
type: SequenceAnnotation
persistent identity: annotation2185215
display id: annotation2185215
version: 1
top level: BBa_K863012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185215/range2185215/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185215/range2185215/1
type: Range
persistent identity: range2185215
display id: range2185215
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1
end: 35
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2185216
display id: annotation2185216
version: 1
top level: BBa_K863012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185216/range2185216/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185216/range2185216/1
type: Range
persistent identity: range2185216
display id: range2185216
version: 1
direction: direction/forward
top level: BBa_K863012
start: 44
end: 55
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2185217
display id: annotation2185217
version: 1
top level: BBa_K863012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185217/range2185217/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185217/range2185217/1
type: Range
persistent identity: range2185217
display id: range2185217
version: 1
direction: direction/forward
top level: BBa_K863012
start: 62
end: 64
orientation: inline

g (original) --> a (mutation)
type: SequenceAnnotation
persistent identity: annotation2185245
display id: annotation2185245
version: 1
top level: BBa_K863012
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185245/range2185245/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185245/range2185245/1
type: Range
persistent identity: range2185245
display id: range2185245
version: 1
direction: direction/forward
top level: BBa_K863012
start: 775
end: 775
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2185224
display id: annotation2185224
version: 1
top level: BBa_K863012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185224/range2185224/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185224/range2185224/1
type: Range
persistent identity: range2185224
display id: range2185224
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1469
end: 1471
orientation: inline

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2185218
display id: annotation2185218
version: 1
top level: BBa_K863012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185218/range2185218/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185218/range2185218/1
type: Range
persistent identity: range2185218
display id: range2185218
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1448
end: 1465
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2185223
display id: annotation2185223
version: 1
top level: BBa_K863012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185223/range2185223/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185223/range2185223/1
type: Range
persistent identity: range2185223
display id: range2185223
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1466
end: 1468
orientation: inline

tthl
type: SequenceAnnotation
persistent identity: annotation2185228
display id: annotation2185228
version: 1
top level: BBa_K863012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185228/range2185228/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185228/range2185228/1
type: Range
persistent identity: range2185228
display id: range2185228
version: 1
direction: direction/forward
top level: BBa_K863012
start: 62
end: 1447
orientation: inline

BBa_K525008
type: ComponentDefinition
persistent identity: BBa K525008
display id: BBa_K525008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of S-Layer SbpA with T7/lac and BisdA and BisdB
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PT7 + RBS + SbpA || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525403 , BBa_K123000 , BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K525401
sequence annotation: Freiburg Scar
sequence annotation: lac operator
sequence annotation: T7
sequence annotation: RBS
sequence annotation: SbpA domain
sequence annotation: B0034
sequence annotation: BisdB
sequence annotation: ATG
sequence annotation: K525515
sequence annotation: BisdA
sequence annotation: NgoMIV
sequence annotation: TAA
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K525008_sequence/1

K525401
type: SequenceAnnotation
persistent identity: annotation2161198
display id: annotation2161198
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161198/range2161198/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161198/range2161198/1
type: Range
persistent identity: range2161198
display id: range2161198
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 3192
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161202
display id: annotation2161202
version: 1
top level: BBa_K525008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161202/range2161202/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161202/range2161202/1
type: Range
persistent identity: range2161202
display id: range2161202
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3193
end: 3198
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161204
display id: annotation2161204
version: 1
top level: BBa_K525008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161204/range2161204/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161204/range2161204/1
type: Range
persistent identity: range2161204
display id: range2161204
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3511
end: 3516
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2161195
display id: annotation2161195
version: 1
top level: BBa_K525008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161195/range2161195/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161195/range2161195/1
type: Range
persistent identity: range2161195
display id: range2161195
version: 1
direction: direction/forward
top level: BBa_K525008
start: 20
end: 44
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2161194
display id: annotation2161194
version: 1
top level: BBa_K525008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161194/range2161194/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161194/range2161194/1
type: Range
persistent identity: range2161194
display id: range2161194
version: 1
direction: direction/forward
top level: BBa_K525008
start: 1
end: 20
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161197
display id: annotation2161197
version: 1
top level: BBa_K525008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161197/range2161197/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161197/range2161197/1
type: Range
persistent identity: range2161197
display id: range2161197
version: 1
direction: direction/forward
top level: BBa_K525008
start: 56
end: 59
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2161200
display id: annotation2161200
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161200/range2161200/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161200/range2161200/1
type: Range
persistent identity: range2161200
display id: range2161200
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 3192
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161196
display id: annotation2161196
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161196/range2161196/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161196/range2161196/1
type: Range
persistent identity: range2161196
display id: range2161196
version: 1
direction: direction/forward
top level: BBa_K525008
start: 53
end: 64
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2161205
display id: annotation2161205
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161205/range2161205/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161205/range2161205/1
type: Range
persistent identity: range2161205
display id: range2161205
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3517
end: 4791
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161199
display id: annotation2161199
version: 1
top level: BBa_K525008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161199/range2161199/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161199/range2161199/1
type: Range
persistent identity: range2161199
display id: range2161199
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 75
orientation: inline

K525515
type: SequenceAnnotation
persistent identity: annotation2161207
display id: annotation2161207
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161207/range2161207/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161207/range2161207/1
type: Range
persistent identity: range2161207
display id: range2161207
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3199
end: 4791
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161203
display id: annotation2161203
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161203/range2161203/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161203/range2161203/1
type: Range
persistent identity: range2161203
display id: range2161203
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3199
end: 3510
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161201
display id: annotation2161201
version: 1
top level: BBa_K525008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161201/range2161201/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161201/range2161201/1
type: Range
persistent identity: range2161201
display id: range2161201
version: 1
direction: direction/forward
top level: BBa_K525008
start: 76
end: 82
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161206
display id: annotation2161206
version: 1
top level: BBa_K525008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161206/range2161206/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161206/range2161206/1
type: Range
persistent identity: range2161206
display id: range2161206
version: 1
direction: direction/forward
top level: BBa_K525008
start: 4789
end: 4791
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161208
display id: annotation2161208
version: 1
top level: BBa_K525008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161208/range2161208/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161208/range2161208/1
type: Range
persistent identity: range2161208
display id: range2161208
version: 1
direction: direction/forward
top level: BBa_K525008
start: 4783
end: 4788
orientation: inline

AAV
type: ComponentDefinition
persistent identity: BBa I11012
display id: BBa_I11012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AAV degradation tag
creator: BU
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: true
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Degradation tag
mutable provenance: Appl Environ Microbiol, 1998 June; 64(6):2240-2246
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_I11012_sequence/1

BBa_K863005
type: ComponentDefinition
persistent identity: BBa K863005
display id: BBa_K863005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ecol laccase from E. coli with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: E.coli laccase ORF with T7, RBS and HIS tag
mutable notes: TEST
mutable provenance: E. coli BL21 (DE3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: 6x HIS
sequence annotation: ecol
sequence annotation: ATG
sequence annotation: RBS
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K863005_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2184563
display id: annotation2184563
version: 1
top level: BBa_K863005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184563/range2184563/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184563/range2184563/1
type: Range
persistent identity: range2184563
display id: range2184563
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1603
end: 1605
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184564
display id: annotation2184564
version: 1
top level: BBa_K863005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184564/range2184564/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184564/range2184564/1
type: Range
persistent identity: range2184564
display id: range2184564
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1600
end: 1602
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2184565
display id: annotation2184565
version: 1
top level: BBa_K863005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184565/range2184565/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184565/range2184565/1
type: Range
persistent identity: range2184565
display id: range2184565
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1582
end: 1599
orientation: inline

ecol
type: SequenceAnnotation
persistent identity: annotation2184566
display id: annotation2184566
version: 1
top level: BBa_K863005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184566/range2184566/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184566/range2184566/1
type: Range
persistent identity: range2184566
display id: range2184566
version: 1
direction: direction/forward
top level: BBa_K863005
start: 34
end: 1581
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184562
display id: annotation2184562
version: 1
top level: BBa_K863005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184562/range2184562/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184562/range2184562/1
type: Range
persistent identity: range2184562
display id: range2184562
version: 1
direction: direction/forward
top level: BBa_K863005
start: 34
end: 36
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2187301
display id: annotation2187301
version: 1
top level: BBa_K863005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2187301/range2187301/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2187301/range2187301/1
type: Range
persistent identity: range2187301
display id: range2187301
version: 1
direction: direction/forward
top level: BBa_K863005
start: 25
end: 30
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2184561
display id: annotation2184561
version: 1
top level: BBa_K863005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184561/range2184561/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184561/range2184561/1
type: Range
persistent identity: range2184561
display id: range2184561
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1
end: 23
orientation: inline

BBa_K863006
type: ComponentDefinition
persistent identity: BBa K863006
display id: BBa_K863006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ecol laccase from E. coli
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: E.coli laccase ORF
mutable notes: test
mutable provenance: E. coli BL21 (DE3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863006_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2184435
display id: annotation2184435
version: 1
top level: BBa_K863006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863006/annotation2184435/range2184435/1

https://synbiohub.org/public/igem/BBa_K863006/annotation2184435/range2184435/1
type: Range
persistent identity: range2184435
display id: range2184435
version: 1
direction: direction/forward
top level: BBa_K863006
start: 1
end: 3
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184436
display id: annotation2184436
version: 1
top level: BBa_K863006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863006/annotation2184436/range2184436/1

https://synbiohub.org/public/igem/BBa_K863006/annotation2184436/range2184436/1
type: Range
persistent identity: range2184436
display id: range2184436
version: 1
direction: direction/forward
top level: BBa_K863006
start: 1549
end: 1551
orientation: inline