//proteindomain/dnabinding
type: Collection
persistent identity: proteindomain dnabinding
display id: proteindomain_dnabinding
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K747045
member: BBa_K801038
member: BBa_K747015
member: BBa_K747087
member: BBa_K364001
member: BBa_K747075
member: BBa_K1855003
member: BBa_K747006
member: BBa_K747036
member: BBa_K747078
member: BBa_K747027
member: BBa_K747057
member: BBa_K801040
member: BBa_K747048
member: BBa_K747069
member: BBa_K747039
member: BBa_K747018
member: BBa_K747000
member: BBa_K747042
member: BBa_K747072
member: BBa_K747030
member: BBa_K165007
member: BBa_K747019
member: BBa_K747084
member: BBa_K801043
member: BBa_K747012
member: BBa_K747041
member: BBa_K747071
member: BBa_K747011
member: BBa_K1031912
member: BBa_K747024
member: BBa_K747054
member: BBa_K747058
member: BBa_K747032
member: BBa_K747053
member: BBa_K747002
member: BBa_K747083
member: BBa_K106007
member: BBa_K747028
member: BBa_K747088
member: BBa_K747062
member: BBa_K747049
member: BBa_K747079
member: BBa_K747092
member: BBa_K801037
member: BBa_K747060
member: BBa_K747090
member: BBa_K747086
member: BBa_K1861050
member: BBa_K801041
member: BBa_K747009
member: BBa_K747077
member: BBa_K618211
member: BBa_K747094
member: BBa_K747081
member: BBa_K747017
member: BBa_K747047
member: BBa_K747051
member: BBa_K747034
member: BBa_K747021
member: BBa_K747064
member: BBa_K1855001
member: BBa_K105003
member: BBa_K747091
member: BBa_K747040
member: BBa_K1861060
member: BBa_K747070
member: BBa_K801042
member: BBa_K747029
member: BBa_K747010
member: BBa_K747008
member: BBa_K801033

BBa_K747034
type: ComponentDefinition
persistent identity: BBa K747034
display id: BBa_K747034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AG3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AG 3
sequence: https://synbiohub.org/public/igem/BBa_K747034_sequence/1

AG 3
type: SequenceAnnotation
persistent identity: annotation2196210
display id: annotation2196210
version: 1
top level: BBa_K747034
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747034/annotation2196210/range2196210/1

https://synbiohub.org/public/igem/BBa_K747034/annotation2196210/range2196210/1
type: Range
persistent identity: range2196210
display id: range2196210
version: 1
direction: direction/forward
top level: BBa_K747034
start: 14
end: 211
orientation: inline

BBa_K747018
type: ComponentDefinition
persistent identity: BBa K747018
display id: BBa_K747018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AG2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AG 2
sequence: https://synbiohub.org/public/igem/BBa_K747018_sequence/1

AG 2
type: SequenceAnnotation
persistent identity: annotation2195969
display id: annotation2195969
version: 1
top level: BBa_K747018
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747018/annotation2195969/range2195969/1

https://synbiohub.org/public/igem/BBa_K747018/annotation2195969/range2195969/1
type: Range
persistent identity: range2195969
display id: range2195969
version: 1
direction: direction/forward
top level: BBa_K747018
start: 14
end: 211
orientation: inline

BBa_K747040
type: ComponentDefinition
persistent identity: BBa K747040
display id: BBa_K747040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 3
sequence: https://synbiohub.org/public/igem/BBa_K747040_sequence/1

GA 3
type: SequenceAnnotation
persistent identity: annotation2196250
display id: annotation2196250
version: 1
top level: BBa_K747040
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747040/annotation2196250/range2196250/1

https://synbiohub.org/public/igem/BBa_K747040/annotation2196250/range2196250/1
type: Range
persistent identity: range2196250
display id: range2196250
version: 1
direction: direction/forward
top level: BBa_K747040
start: 14
end: 211
orientation: inline

BBa_K747088
type: ComponentDefinition
persistent identity: BBa K747088
display id: BBa_K747088
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 6
sequence: https://synbiohub.org/public/igem/BBa_K747088_sequence/1

GA 6
type: SequenceAnnotation
persistent identity: annotation2197064
display id: annotation2197064
version: 1
top level: BBa_K747088
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747088/annotation2197064/range2197064/1

https://synbiohub.org/public/igem/BBa_K747088/annotation2197064/range2197064/1
type: Range
persistent identity: range2197064
display id: range2197064
version: 1
direction: direction/forward
top level: BBa_K747088
start: 14
end: 211
orientation: inline

BBa_K105004
type: ComponentDefinition
persistent identity: BBa K105004
display id: BBa_K105004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI - repressor from E.coli phage lambda
creator: Katja Karstens
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: cI is a repressor of the phage lamda. It binds specificly to a operator site ( BBa_R0051 & BBa_K105021).
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: We changed the format of the cI coding part of BBa_C0051 into Phillips/Silver standard.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K105004_sequence/1

BBa_K801038
type: ComponentDefinition
persistent identity: BBa K801038
display id: BBa_K801038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhyB-linker-Gal4bd
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K801031, Bba_K365005 and BBa_K801032
mutable notes: -
mutable provenance: Composite part of BBa_K801031, Bba_K365005 and BBa_K801032
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin (PCB)
sequence annotation: Essential Arg for photoconvertible binding of PIF3
sequence annotation: BBa_K801032
sequence annotation: BBa_K365005
sequence annotation: Essential Gly for photoconvertible binding of PIF3
sequence annotation: BBa_K801031
sequence annotation: RFC25 fusion scar
sequence annotation: BBa_K801037
sequence: https://synbiohub.org/public/igem/BBa_K801038_sequence/1

Essential Cys for chromophore attachement of phycocyanobilin (PCB)
type: SequenceAnnotation
persistent identity: annotation2198916
display id: annotation2198916
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198916/range2198916/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198916/range2198916/1
type: Range
persistent identity: range2198916
display id: range2198916
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1066
end: 1068
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198912
display id: annotation2198912
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198912/range2198912/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198912/range2198912/1
type: Range
persistent identity: range2198912
display id: range2198912
version: 1
direction: direction/forward
top level: BBa_K801038
start: 325
end: 327
orientation: inline

BBa_K801032
type: SequenceAnnotation
persistent identity: annotation2198920
display id: annotation2198920
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198920/range2198920/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198920/range2198920/1
type: Range
persistent identity: range2198920
display id: range2198920
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2794
end: 3231
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2198918
display id: annotation2198918
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198918/range2198918/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198918/range2198918/1
type: Range
persistent identity: range2198918
display id: range2198918
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2728
end: 2787
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198913
display id: annotation2198913
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198913/range2198913/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198913/range2198913/1
type: Range
persistent identity: range2198913
display id: range2198913
version: 1
direction: direction/forward
top level: BBa_K801038
start: 328
end: 330
orientation: inline

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2198917
display id: annotation2198917
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198917/range2198917/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198917/range2198917/1
type: Range
persistent identity: range2198917
display id: range2198917
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1
end: 2721
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198919
display id: annotation2198919
version: 1
top level: BBa_K801038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198919/range2198919/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198919/range2198919/1
type: Range
persistent identity: range2198919
display id: range2198919
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2788
end: 2793
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198923
display id: annotation2198923
version: 1
top level: BBa_K801038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198923/range2198923/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198923/range2198923/1
type: Range
persistent identity: range2198923
display id: range2198923
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2722
end: 2727
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198914
display id: annotation2198914
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198914/range2198914/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198914/range2198914/1
type: Range
persistent identity: range2198914
display id: range2198914
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1039
end: 1041
orientation: inline

BBa_K801037
type: SequenceAnnotation
persistent identity: annotation2198921
display id: annotation2198921
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198921/range2198921/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198921/range2198921/1
type: Range
persistent identity: range2198921
display id: range2198921
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2728
end: 3231
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198915
display id: annotation2198915
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198915/range2198915/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198915/range2198915/1
type: Range
persistent identity: range2198915
display id: range2198915
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1051
end: 1053
orientation: inline

BBa_K747012
type: ComponentDefinition
persistent identity: BBa K747012
display id: BBa_K747012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 1
sequence: https://synbiohub.org/public/igem/BBa_K747012_sequence/1

TA 1
type: SequenceAnnotation
persistent identity: annotation2195920
display id: annotation2195920
version: 1
top level: BBa_K747012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747012/annotation2195920/range2195920/1

https://synbiohub.org/public/igem/BBa_K747012/annotation2195920/range2195920/1
type: Range
persistent identity: range2195920
display id: range2195920
version: 1
direction: direction/forward
top level: BBa_K747012
start: 12
end: 209
orientation: inline

BBa_K747015
type: ComponentDefinition
persistent identity: BBa K747015
display id: BBa_K747015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 1
sequence: https://synbiohub.org/public/igem/BBa_K747015_sequence/1

TT 1
type: SequenceAnnotation
persistent identity: annotation2195931
display id: annotation2195931
version: 1
top level: BBa_K747015
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747015/annotation2195931/range2195931/1

https://synbiohub.org/public/igem/BBa_K747015/annotation2195931/range2195931/1
type: Range
persistent identity: range2195931
display id: range2195931
version: 1
direction: direction/forward
top level: BBa_K747015
start: 12
end: 209
orientation: inline

BBa_K747039
type: ComponentDefinition
persistent identity: BBa K747039
display id: BBa_K747039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CT3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CT 3
sequence: https://synbiohub.org/public/igem/BBa_K747039_sequence/1

CT 3
type: SequenceAnnotation
persistent identity: annotation2196224
display id: annotation2196224
version: 1
top level: BBa_K747039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747039/annotation2196224/range2196224/1

https://synbiohub.org/public/igem/BBa_K747039/annotation2196224/range2196224/1
type: Range
persistent identity: range2196224
display id: range2196224
version: 1
direction: direction/forward
top level: BBa_K747039
start: 14
end: 211
orientation: inline

BBa_K747045
type: ComponentDefinition
persistent identity: BBa K747045
display id: BBa_K747045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 3
sequence: https://synbiohub.org/public/igem/BBa_K747045_sequence/1

TC 3
type: SequenceAnnotation
persistent identity: annotation2196264
display id: annotation2196264
version: 1
top level: BBa_K747045
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747045/annotation2196264/range2196264/1

https://synbiohub.org/public/igem/BBa_K747045/annotation2196264/range2196264/1
type: Range
persistent identity: range2196264
display id: range2196264
version: 1
direction: direction/forward
top level: BBa_K747045
start: 14
end: 211
orientation: inline

BBa_K747027
type: ComponentDefinition
persistent identity: BBa K747027
display id: BBa_K747027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 2
sequence: https://synbiohub.org/public/igem/BBa_K747027_sequence/1

GT 2
type: SequenceAnnotation
persistent identity: annotation2196136
display id: annotation2196136
version: 1
top level: BBa_K747027
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747027/annotation2196136/range2196136/1

https://synbiohub.org/public/igem/BBa_K747027/annotation2196136/range2196136/1
type: Range
persistent identity: range2196136
display id: range2196136
version: 1
direction: direction/forward
top level: BBa_K747027
start: 14
end: 211
orientation: inline

BBa_K747061
type: ComponentDefinition
persistent identity: BBa K747061
display id: BBa_K747061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 4
sequence: https://synbiohub.org/public/igem/BBa_K747061_sequence/1

TC 4
type: SequenceAnnotation
persistent identity: annotation2197018
display id: annotation2197018
version: 1
top level: BBa_K747061
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747061/annotation2197018/range2197018/1

https://synbiohub.org/public/igem/BBa_K747061/annotation2197018/range2197018/1
type: Range
persistent identity: range2197018
display id: range2197018
version: 1
direction: direction/forward
top level: BBa_K747061
start: 14
end: 211
orientation: inline

BBa_K747072
type: ComponentDefinition
persistent identity: BBa K747072
display id: BBa_K747072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 5
sequence: https://synbiohub.org/public/igem/BBa_K747072_sequence/1

GA 5
type: SequenceAnnotation
persistent identity: annotation2197039
display id: annotation2197039
version: 1
top level: BBa_K747072
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747072/annotation2197039/range2197039/1

https://synbiohub.org/public/igem/BBa_K747072/annotation2197039/range2197039/1
type: Range
persistent identity: range2197039
display id: range2197039
version: 1
direction: direction/forward
top level: BBa_K747072
start: 14
end: 211
orientation: inline

BBa_K801042
type: ComponentDefinition
persistent identity: BBa K801042
display id: BBa_K801042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GAL4 based yeast light-switchable promoter system
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011
mutable notes: -
mutable provenance: composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: SV40NLS-PhyB-Linker-GAL4DBD
sequence annotation: HA epitope tage
sequence annotation: T7 promoter (for sequencing)
sequence annotation: BBa_K319003 (pTEF1, TEF1 promoter)
sequence annotation: BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD)
sequence annotation: Linker
sequence annotation: GAL4DBD (GAL4 DNA binding domain)
sequence annotation: BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
sequence annotation: GAL4AD (GAL4 transcriptonal activation domain)
sequence annotation: Phytochrome B (PhyB, 908 first N-terminal amino acids)
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: SV40 NLS (SV40 nuclear localization signal)
sequence annotation: 20 amino acids long linker (BBa_K365005)
sequence annotation: BBa_K801011, (tTEF1, TEF1 terminator)
sequence: https://synbiohub.org/public/igem/BBa_K801042_sequence/1

SV40NLS-PhyB-Linker-GAL4DBD
type: SequenceAnnotation
persistent identity: annotation2198791
display id: annotation2198791
version: 1
top level: BBa_K801042
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198791/range2198791/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198791/range2198791/1
type: Range
persistent identity: range2198791
display id: range2198791
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2237
end: 5548
orientation: inline

HA epitope tage
type: SequenceAnnotation
persistent identity: annotation2198787
display id: annotation2198787
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198787/range2198787/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198787/range2198787/1
type: Range
persistent identity: range2198787
display id: range2198787
version: 1
direction: direction/forward
top level: BBa_K801042
start: 912
end: 932
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2198786
display id: annotation2198786
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198786/range2198786/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198786/range2198786/1
type: Range
persistent identity: range2198786
display id: range2198786
version: 1
direction: direction/forward
top level: BBa_K801042
start: 869
end: 891
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198790
display id: annotation2198790
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198790/range2198790/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198790/range2198790/1
type: Range
persistent identity: range2198790
display id: range2198790
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1782
end: 2231
orientation: inline

BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD)
type: SequenceAnnotation
persistent identity: annotation2198793
display id: annotation2198793
version: 1
top level: BBa_K801042
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198793/range2198793/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198793/range2198793/1
type: Range
persistent identity: range2198793
display id: range2198793
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2246
end: 5539
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2198785
display id: annotation2198785
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198785/range2198785/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198785/range2198785/1
type: Range
persistent identity: range2198785
display id: range2198785
version: 1
direction: direction/forward
top level: BBa_K801042
start: 864
end: 959
orientation: inline

GAL4DBD (GAL4 DNA binding domain)
type: SequenceAnnotation
persistent identity: annotation2198796
display id: annotation2198796
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198796/range2198796/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198796/range2198796/1
type: Range
persistent identity: range2198796
display id: range2198796
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5102
end: 5539
orientation: inline

BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
type: SequenceAnnotation
persistent identity: annotation2198782
display id: annotation2198782
version: 1
top level: BBa_K801042
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198782/range2198782/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198782/range2198782/1
type: Range
persistent identity: range2198782
display id: range2198782
version: 1
direction: direction/forward
top level: BBa_K801042
start: 456
end: 1259
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198781
display id: annotation2198781
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198781/range2198781/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198781/range2198781/1
type: Range
persistent identity: range2198781
display id: range2198781
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1
end: 450
orientation: inline

GAL4AD (GAL4 transcriptonal activation domain)
type: SequenceAnnotation
persistent identity: annotation2198784
display id: annotation2198784
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198784/range2198784/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198784/range2198784/1
type: Range
persistent identity: range2198784
display id: range2198784
version: 1
direction: direction/forward
top level: BBa_K801042
start: 525
end: 863
orientation: inline

Phytochrome B (PhyB, 908 first N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198794
display id: annotation2198794
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198794/range2198794/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198794/range2198794/1
type: Range
persistent identity: range2198794
display id: range2198794
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2309
end: 5029
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198788
display id: annotation2198788
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198788/range2198788/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198788/range2198788/1
type: Range
persistent identity: range2198788
display id: range2198788
version: 1
direction: direction/forward
top level: BBa_K801042
start: 960
end: 1256
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198792
display id: annotation2198792
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198792/range2198792/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198792/range2198792/1
type: Range
persistent identity: range2198792
display id: range2198792
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2246
end: 2302
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198783
display id: annotation2198783
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198783/range2198783/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198783/range2198783/1
type: Range
persistent identity: range2198783
display id: range2198783
version: 1
direction: direction/forward
top level: BBa_K801042
start: 465
end: 521
orientation: inline

20 amino acids long linker (BBa_K365005)
type: SequenceAnnotation
persistent identity: annotation2198795
display id: annotation2198795
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198795/range2198795/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198795/range2198795/1
type: Range
persistent identity: range2198795
display id: range2198795
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5036
end: 5095
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198816
display id: annotation2198816
version: 1
top level: BBa_K801042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198816/range2198816/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198816/range2198816/1
type: Range
persistent identity: range2198816
display id: range2198816
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5557
end: 6063
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198789
display id: annotation2198789
version: 1
top level: BBa_K801042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198789/range2198789/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198789/range2198789/1
type: Range
persistent identity: range2198789
display id: range2198789
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1268
end: 1774
orientation: inline

BBa_K1855001
type: ComponentDefinition
persistent identity: BBa K1855001
display id: BBa_K1855001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lpp-OmpA sZF2
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:11:22Z
discontinued: false
dominant: false
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (sZF2),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to TGCTGCTTC on the cell surface of E.coli.
mutable notes: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The Lpp-OmpA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The sZF2 domain is preceeded by an NheI cut site and followed by a ClaI cut site.
mutable provenance: Lpp-OmpA is originally from E.coli; sZF2 is from http://www.nature.com/nmeth/journal/v10/n5/abs/nmeth.2407.html
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sZF2
sequence annotation: B0034 Strong RBS
sequence annotation: 3x FLAG
sequence annotation: pL_lac
sequence annotation: Lpp-OmpA
sequence annotation: BBa_K864600 T0 Terminator
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K1855001_sequence/1

sZF2
type: SequenceAnnotation
persistent identity: annotation2466711
display id: annotation2466711
version: 1
top level: BBa_K1855001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466711/range2466711/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466711/range2466711/1
type: Range
persistent identity: range2466711
display id: range2466711
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 564
end: 833
orientation: inline

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466694
display id: annotation2466694
version: 1
top level: BBa_K1855001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466694/range2466694/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466694/range2466694/1
type: Range
persistent identity: range2466694
display id: range2466694
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 60
end: 80
orientation: inline

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466713
display id: annotation2466713
version: 1
top level: BBa_K1855001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466713/range2466713/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466713/range2466713/1
type: Range
persistent identity: range2466713
display id: range2466713
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 867
end: 938
orientation: inline

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466693
display id: annotation2466693
version: 1
top level: BBa_K1855001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466693/range2466693/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466693/range2466693/1
type: Range
persistent identity: range2466693
display id: range2466693
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 1
end: 56
orientation: inline

Lpp-OmpA
type: SequenceAnnotation
persistent identity: annotation2466695
display id: annotation2466695
version: 1
top level: BBa_K1855001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466695/range2466695/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466695/range2466695/1
type: Range
persistent identity: range2466695
display id: range2466695
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 84
end: 518
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466714
display id: annotation2466714
version: 1
top level: BBa_K1855001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466714/range2466714/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466714/range2466714/1
type: Range
persistent identity: range2466714
display id: range2466714
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 945
end: 996
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466712
display id: annotation2466712
version: 1
top level: BBa_K1855001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466712/range2466712/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466712/range2466712/1
type: Range
persistent identity: range2466712
display id: range2466712
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 840
end: 863
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466710
display id: annotation2466710
version: 1
top level: BBa_K1855001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466710/range2466710/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466710/range2466710/1
type: Range
persistent identity: range2466710
display id: range2466710
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 522
end: 557
orientation: inline

BBa_K747074
type: ComponentDefinition
persistent identity: BBa K747074
display id: BBa_K747074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 5
sequence: https://synbiohub.org/public/igem/BBa_K747074_sequence/1

GG 5
type: SequenceAnnotation
persistent identity: annotation2197043
display id: annotation2197043
version: 1
top level: BBa_K747074
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747074/annotation2197043/range2197043/1

https://synbiohub.org/public/igem/BBa_K747074/annotation2197043/range2197043/1
type: Range
persistent identity: range2197043
display id: range2197043
version: 1
direction: direction/forward
top level: BBa_K747074
start: 14
end: 211
orientation: inline

BBa_K747035
type: ComponentDefinition
persistent identity: BBa K747035
display id: BBa_K747035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 3
sequence: https://synbiohub.org/public/igem/BBa_K747035_sequence/1

AT 3
type: SequenceAnnotation
persistent identity: annotation2196211
display id: annotation2196211
version: 1
top level: BBa_K747035
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747035/annotation2196211/range2196211/1

https://synbiohub.org/public/igem/BBa_K747035/annotation2196211/range2196211/1
type: Range
persistent identity: range2196211
display id: range2196211
version: 1
direction: direction/forward
top level: BBa_K747035
start: 14
end: 211
orientation: inline

BBa_K747041
type: ComponentDefinition
persistent identity: BBa K747041
display id: BBa_K747041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 3
sequence: https://synbiohub.org/public/igem/BBa_K747041_sequence/1

GC 3
type: SequenceAnnotation
persistent identity: annotation2196252
display id: annotation2196252
version: 1
top level: BBa_K747041
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747041/annotation2196252/range2196252/1

https://synbiohub.org/public/igem/BBa_K747041/annotation2196252/range2196252/1
type: Range
persistent identity: range2196252
display id: range2196252
version: 1
direction: direction/forward
top level: BBa_K747041
start: 14
end: 211
orientation: inline

BBa_K747008
type: ComponentDefinition
persistent identity: BBa K747008
display id: BBa_K747008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 1
sequence: https://synbiohub.org/public/igem/BBa_K747008_sequence/1

GA 1
type: SequenceAnnotation
persistent identity: annotation2195824
display id: annotation2195824
version: 1
top level: BBa_K747008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747008/annotation2195824/range2195824/1

https://synbiohub.org/public/igem/BBa_K747008/annotation2195824/range2195824/1
type: Range
persistent identity: range2195824
display id: range2195824
version: 1
direction: direction/forward
top level: BBa_K747008
start: 12
end: 209
orientation: inline

BBa_K747029
type: ComponentDefinition
persistent identity: BBa K747029
display id: BBa_K747029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 2
sequence: https://synbiohub.org/public/igem/BBa_K747029_sequence/1

TC 2
type: SequenceAnnotation
persistent identity: annotation2196201
display id: annotation2196201
version: 1
top level: BBa_K747029
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747029/annotation2196201/range2196201/1

https://synbiohub.org/public/igem/BBa_K747029/annotation2196201/range2196201/1
type: Range
persistent identity: range2196201
display id: range2196201
version: 1
direction: direction/forward
top level: BBa_K747029
start: 14
end: 211
orientation: inline

BBa_K909007
type: ComponentDefinition
persistent identity: BBa K909007
display id: BBa_K909007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR DNA binding domain containing BamHI site for protein fusions
creator: Gintautas Vainorius
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1174_
m user id: 0
owner id: 14094
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Truncated version of tetracycline repressor TetR, consisting of 1 ??? 127 amino acids, containing DNA binding domain. The rest of the structure, 8-10 helixes responsible for tetR dimerization, were removed in order to keep tetR-DBD in monomer form and prevent from efficient DNA binding and transcription repression. In addition, we introduced BamHI site at C terminus for an easy in tetR-DBD frame fusions with other proteins. Thus, it can be used in two hybrid systems for both, homo and hetero dimerizations, also one can use these fusions to turn protein-protein interaction into the repression of transcription.
mutable notes: Deletion of 8-10 helixes might lower the stability of protein, also TetR-DBD is no longer responsive to anhydrotetracycline
mutable provenance: E. coli Tn10 transposon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: BamHI restriction site
sequence: https://synbiohub.org/public/igem/BBa_K909007_sequence/1

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation2194604
display id: annotation2194604
version: 1
top level: BBa_K909007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909007/annotation2194604/range2194604/1

https://synbiohub.org/public/igem/BBa_K909007/annotation2194604/range2194604/1
type: Range
persistent identity: range2194604
display id: range2194604
version: 1
direction: direction/forward
top level: BBa_K909007
start: 382
end: 387
orientation: inline

BBa_K747051
type: ComponentDefinition
persistent identity: BBa K747051
display id: BBa_K747051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT4_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 4
sequence: https://synbiohub.org/public/igem/BBa_K747051_sequence/1

AT 4
type: SequenceAnnotation
persistent identity: annotation2196280
display id: annotation2196280
version: 1
top level: BBa_K747051
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747051/annotation2196280/range2196280/1

https://synbiohub.org/public/igem/BBa_K747051/annotation2196280/range2196280/1
type: Range
persistent identity: range2196280
display id: range2196280
version: 1
direction: direction/forward
top level: BBa_K747051
start: 14
end: 211
orientation: inline

BBa_K747077
type: ComponentDefinition
persistent identity: BBa K747077
display id: BBa_K747077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 5
sequence: https://synbiohub.org/public/igem/BBa_K747077_sequence/1

TC 5
type: SequenceAnnotation
persistent identity: annotation2197046
display id: annotation2197046
version: 1
top level: BBa_K747077
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747077/annotation2197046/range2197046/1

https://synbiohub.org/public/igem/BBa_K747077/annotation2197046/range2197046/1
type: Range
persistent identity: range2197046
display id: range2197046
version: 1
direction: direction/forward
top level: BBa_K747077
start: 14
end: 211
orientation: inline

BBa_K747013
type: ComponentDefinition
persistent identity: BBa K747013
display id: BBa_K747013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 1
sequence: https://synbiohub.org/public/igem/BBa_K747013_sequence/1

TC 1
type: SequenceAnnotation
persistent identity: annotation2195923
display id: annotation2195923
version: 1
top level: BBa_K747013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747013/annotation2195923/range2195923/1

https://synbiohub.org/public/igem/BBa_K747013/annotation2195923/range2195923/1
type: Range
persistent identity: range2195923
display id: range2195923
version: 1
direction: direction/forward
top level: BBa_K747013
start: 12
end: 209
orientation: inline

BBa_K618211
type: ComponentDefinition
persistent identity: BBa K618211
display id: BBa_K618211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLAC ompA-Gli1
creator: Ben Park, Chris Bate, Marc Ammerlaan
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _790_
m user id: 0
owner id: 8977
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a fusion between ompA and the DNA binding protein Gli-1 under the control of pLAC. The protein should be present on the outer surface of cells and bind the Gli-1 recognition sequence (GACCACCCAAGACGA). This will also allow cells to be anchored to a glass slide with the appropriate DNA attached. This can serve as a way to position cells in any desired pattern.
mutable notes: NOTE- the sequence as listed here is wrong. Because part K165007 is supplied on a Silver lab vector (Bba_J63009 or V0002) with a modified biobrick prefix, the scar between ompA and Gli-1 is non-standard. The G at position 840 is not there. This has been verified by sequencing. Deleting the G keeps Gli-1 in frame with ompA. Part length is 1364bp. The sequence was generated automatically and I couldn't figure out how to edit it.
mutable provenance: parts come from iGEM distribution and from iGEM HQ
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J04500
component: BBa_K165007
component: BBa_K103006
sequence annotation: BBa_K165007
sequence annotation: BBa_K103006
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K618211_sequence/1

BBa_J04500
type: Component
persistent identity: component2144971
display id: component2144971
version: 1
top level: BBa_K618211
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K165007
type: Component
persistent identity: component2144978
display id: component2144978
version: 1
top level: BBa_K618211
access: public
definition: BBa_K165007

BBa_K103006
type: Component
persistent identity: component2144977
display id: component2144977
version: 1
top level: BBa_K618211
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation2144978
display id: annotation2144978
version: 1
top level: BBa_K618211
location: https://synbiohub.org/public/igem/BBa_K618211/annotation2144978/range2144978/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K618211/annotation2144978/range2144978/1
type: Range
persistent identity: range2144978
display id: range2144978
version: 1
direction: direction/reverse
top level: BBa_K618211
start: 701
end: 1225
orientation: inline

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation2144977
display id: annotation2144977
version: 1
top level: BBa_K618211
location: https://synbiohub.org/public/igem/BBa_K618211/annotation2144977/range2144977/1
component: BBa_K103006

https://synbiohub.org/public/igem/BBa_K618211/annotation2144977/range2144977/1
type: Range
persistent identity: range2144977
display id: range2144977
version: 1
direction: direction/reverse
top level: BBa_K618211
start: 229
end: 692
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2144971
display id: annotation2144971
version: 1
top level: BBa_K618211
location: https://synbiohub.org/public/igem/BBa_K618211/annotation2144971/range2144971/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K618211/annotation2144971/range2144971/1
type: Range
persistent identity: range2144971
display id: range2144971
version: 1
direction: direction/reverse
top level: BBa_K618211
start: 1
end: 220
orientation: inline

BBa_K747055
type: ComponentDefinition
persistent identity: BBa K747055
display id: BBa_K747055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CT4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CT 4
sequence: https://synbiohub.org/public/igem/BBa_K747055_sequence/1

CT 4
type: SequenceAnnotation
persistent identity: annotation2197012
display id: annotation2197012
version: 1
top level: BBa_K747055
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747055/annotation2197012/range2197012/1

https://synbiohub.org/public/igem/BBa_K747055/annotation2197012/range2197012/1
type: Range
persistent identity: range2197012
display id: range2197012
version: 1
direction: direction/forward
top level: BBa_K747055
start: 14
end: 211
orientation: inline

BBa_K747086
type: ComponentDefinition
persistent identity: BBa K747086
display id: BBa_K747086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 6
sequence: https://synbiohub.org/public/igem/BBa_K747086_sequence/1

CG 6
type: SequenceAnnotation
persistent identity: annotation2197060
display id: annotation2197060
version: 1
top level: BBa_K747086
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747086/annotation2197060/range2197060/1

https://synbiohub.org/public/igem/BBa_K747086/annotation2197060/range2197060/1
type: Range
persistent identity: range2197060
display id: range2197060
version: 1
direction: direction/forward
top level: BBa_K747086
start: 14
end: 211
orientation: inline

BBa_K747090
type: ComponentDefinition
persistent identity: BBa K747090
display id: BBa_K747090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 6
sequence: https://synbiohub.org/public/igem/BBa_K747090_sequence/1

GG 6
type: SequenceAnnotation
persistent identity: annotation2197066
display id: annotation2197066
version: 1
top level: BBa_K747090
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747090/annotation2197066/range2197066/1

https://synbiohub.org/public/igem/BBa_K747090/annotation2197066/range2197066/1
type: Range
persistent identity: range2197066
display id: range2197066
version: 1
direction: direction/forward
top level: BBa_K747090
start: 14
end: 211
orientation: inline

BBa_K747058
type: ComponentDefinition
persistent identity: BBa K747058
display id: BBa_K747058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 4
sequence: https://synbiohub.org/public/igem/BBa_K747058_sequence/1

GG 4
type: SequenceAnnotation
persistent identity: annotation2197015
display id: annotation2197015
version: 1
top level: BBa_K747058
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747058/annotation2197015/range2197015/1

https://synbiohub.org/public/igem/BBa_K747058/annotation2197015/range2197015/1
type: Range
persistent identity: range2197015
display id: range2197015
version: 1
direction: direction/forward
top level: BBa_K747058
start: 14
end: 211
orientation: inline

BBa_K106007
type: ComponentDefinition
persistent identity: BBa K106007
display id: BBa_K106007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA (1-87) DBD, Aar1 AB part
creator: Willis Wong
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3412
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a coding sequence for the partial LexA gene with AB ends for Aar1 cloning.
mutable notes: Since this is an AB part, there is a ATG but no stop codon. For more information on AarI cloning, please refer to the UCSF 2008 iGEM page.
mutable provenance: Yeast genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K106007_sequence/1

BBa_K747031
type: ComponentDefinition
persistent identity: BBa K747031
display id: BBa_K747031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 2
sequence: https://synbiohub.org/public/igem/BBa_K747031_sequence/1

TT 2
type: SequenceAnnotation
persistent identity: annotation2196206
display id: annotation2196206
version: 1
top level: BBa_K747031
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747031/annotation2196206/range2196206/1

https://synbiohub.org/public/igem/BBa_K747031/annotation2196206/range2196206/1
type: Range
persistent identity: range2196206
display id: range2196206
version: 1
direction: direction/forward
top level: BBa_K747031
start: 14
end: 211
orientation: inline

BBa_K747084
type: ComponentDefinition
persistent identity: BBa K747084
display id: BBa_K747084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CA6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CA 6
sequence: https://synbiohub.org/public/igem/BBa_K747084_sequence/1

CA 6
type: SequenceAnnotation
persistent identity: annotation2197057
display id: annotation2197057
version: 1
top level: BBa_K747084
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747084/annotation2197057/range2197057/1

https://synbiohub.org/public/igem/BBa_K747084/annotation2197057/range2197057/1
type: Range
persistent identity: range2197057
display id: range2197057
version: 1
direction: direction/forward
top level: BBa_K747084
start: 14
end: 211
orientation: inline

BBa_K747009
type: ComponentDefinition
persistent identity: BBa K747009
display id: BBa_K747009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 1
sequence: https://synbiohub.org/public/igem/BBa_K747009_sequence/1

GC 1
type: SequenceAnnotation
persistent identity: annotation2195828
display id: annotation2195828
version: 1
top level: BBa_K747009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747009/annotation2195828/range2195828/1

https://synbiohub.org/public/igem/BBa_K747009/annotation2195828/range2195828/1
type: Range
persistent identity: range2195828
display id: range2195828
version: 1
direction: direction/forward
top level: BBa_K747009
start: 12
end: 209
orientation: inline

BBa_K747076
type: ComponentDefinition
persistent identity: BBa K747076
display id: BBa_K747076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This Part was synthesized and flanked with BsmBI-restriction sites.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 5
sequence: https://synbiohub.org/public/igem/BBa_K747076_sequence/1

TA 5
type: SequenceAnnotation
persistent identity: annotation2197045
display id: annotation2197045
version: 1
top level: BBa_K747076
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747076/annotation2197045/range2197045/1

https://synbiohub.org/public/igem/BBa_K747076/annotation2197045/range2197045/1
type: Range
persistent identity: range2197045
display id: range2197045
version: 1
direction: direction/forward
top level: BBa_K747076
start: 14
end: 211
orientation: inline

BBa_K1861060
type: ComponentDefinition
persistent identity: BBa K1861060
display id: BBa_K1861060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of TALE2 and a SpyTag in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Why can't I edit these descriptions?
mutable notes: Coming soon, if I am allowed to edit this.
mutable provenance: It keeps saying I have no permission to edit this.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1861101
component: BBa_K1861102
component: BBa_K1159201
component: BBa_K392003
component: BBa_K1861104
component: BBa_K1861601
sequence annotation: BBa_K1861104
sequence annotation: BBa_K1159201
sequence annotation: BBa_K1861102
sequence annotation: BBa_K1861101
sequence annotation: BBa_K392003
sequence annotation: BBa_K1861601
sequence: https://synbiohub.org/public/igem/BBa_K1861060_sequence/1

BBa_K1861101
type: Component
persistent identity: component2475040
display id: component2475040
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1861102
type: Component
persistent identity: component2475041
display id: component2475041
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K1159201
type: Component
persistent identity: component2475045
display id: component2475045
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K392003
type: Component
persistent identity: component2475047
display id: component2475047
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K1861104
type: Component
persistent identity: component2475046
display id: component2475046
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1861601
type: Component
persistent identity: component2475042
display id: component2475042
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861601/1

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2475046
display id: annotation2475046
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475046/range2475046/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475046/range2475046/1
type: Range
persistent identity: range2475046
display id: range2475046
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 2515
end: 2523
orientation: inline

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2475045
display id: annotation2475045
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475045/range2475045/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475045/range2475045/1
type: Range
persistent identity: range2475045
display id: range2475045
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 2476
end: 2514
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2475041
display id: annotation2475041
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475041/range2475041/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475041/range2475041/1
type: Range
persistent identity: range2475041
display id: range2475041
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 239
end: 265
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2475040
display id: annotation2475040
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475040/range2475040/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475040/range2475040/1
type: Range
persistent identity: range2475040
display id: range2475040
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 1
end: 238
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2475047
display id: annotation2475047
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475047/range2475047/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475047/range2475047/1
type: Range
persistent identity: range2475047
display id: range2475047
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 2524
end: 2652
orientation: inline

BBa_K1861601
type: SequenceAnnotation
persistent identity: annotation2475042
display id: annotation2475042
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475042/range2475042/1
component: BBa_K1861601

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475042/range2475042/1
type: Range
persistent identity: range2475042
display id: range2475042
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 266
end: 2475
orientation: inline

BBa_K747042
type: ComponentDefinition
persistent identity: BBa K747042
display id: BBa_K747042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 3
sequence: https://synbiohub.org/public/igem/BBa_K747042_sequence/1

GG 3
type: SequenceAnnotation
persistent identity: annotation2196258
display id: annotation2196258
version: 1
top level: BBa_K747042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747042/annotation2196258/range2196258/1

https://synbiohub.org/public/igem/BBa_K747042/annotation2196258/range2196258/1
type: Range
persistent identity: range2196258
display id: range2196258
version: 1
direction: direction/forward
top level: BBa_K747042
start: 14
end: 211
orientation: inline

BBa_K747068
type: ComponentDefinition
persistent identity: BBa K747068
display id: BBa_K747068
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CA5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CA 5
sequence: https://synbiohub.org/public/igem/BBa_K747068_sequence/1

CA 5
type: SequenceAnnotation
persistent identity: annotation2197031
display id: annotation2197031
version: 1
top level: BBa_K747068
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747068/annotation2197031/range2197031/1

https://synbiohub.org/public/igem/BBa_K747068/annotation2197031/range2197031/1
type: Range
persistent identity: range2197031
display id: range2197031
version: 1
direction: direction/forward
top level: BBa_K747068
start: 14
end: 211
orientation: inline

BBa_K747005
type: ComponentDefinition
persistent identity: BBa K747005
display id: BBa_K747005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cgtctcaTGACcccggaacaggtggtggccattgcatctcacgatggtggtAagcaggccttggagaccgttcagcgcCt gCtgccagtgCtgtgtcaggcccatggcctcacaccggaacaggtggtggccatcgccagccacgacggcggcaaacagg ctCtggaaactgtgcagcgcCtgttgccggtgCtgtgtcaggcccacggGCTCtgagacg
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 1
sequence: https://synbiohub.org/public/igem/BBa_K747005_sequence/1

CC 1
type: SequenceAnnotation
persistent identity: annotation2195792
display id: annotation2195792
version: 1
top level: BBa_K747005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747005/annotation2195792/range2195792/1

https://synbiohub.org/public/igem/BBa_K747005/annotation2195792/range2195792/1
type: Range
persistent identity: range2195792
display id: range2195792
version: 1
direction: direction/forward
top level: BBa_K747005
start: 12
end: 209
orientation: inline

BBa_K747010
type: ComponentDefinition
persistent identity: BBa K747010
display id: BBa_K747010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 1
sequence: https://synbiohub.org/public/igem/BBa_K747010_sequence/1

GG 1
type: SequenceAnnotation
persistent identity: annotation2195829
display id: annotation2195829
version: 1
top level: BBa_K747010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747010/annotation2195829/range2195829/1

https://synbiohub.org/public/igem/BBa_K747010/annotation2195829/range2195829/1
type: Range
persistent identity: range2195829
display id: range2195829
version: 1
direction: direction/forward
top level: BBa_K747010
start: 12
end: 209
orientation: inline

BBa_K1855005
type: ComponentDefinition
persistent identity: BBa K1855005
display id: BBa_K1855005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BclA-Zif268
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:13:15Z
discontinued: false
dominant: false
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (BclA) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli.
mutable notes: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The BclA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The Zif268 fragment domain is preceeded by an NheI cut site and followed by a ClaI cut site.
mutable provenance: BclA is originally from Bacillus Anthracis; Zif268 is from mice.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0034 Strong RBS
sequence annotation: 3x FLAG
sequence annotation: Zif268
sequence annotation: BBa_K864600 T0 Terminator
sequence annotation: pL_lac
sequence annotation: Linker
sequence annotation: BclA
sequence: https://synbiohub.org/public/igem/BBa_K1855005_sequence/1

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466910
display id: annotation2466910
version: 1
top level: BBa_K1855005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466910/range2466910/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466910/range2466910/1
type: Range
persistent identity: range2466910
display id: range2466910
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 60
end: 80
orientation: inline

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466915
display id: annotation2466915
version: 1
top level: BBa_K1855005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466915/range2466915/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466915/range2466915/1
type: Range
persistent identity: range2466915
display id: range2466915
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 492
end: 563
orientation: inline

Zif268
type: SequenceAnnotation
persistent identity: annotation2466913
display id: annotation2466913
version: 1
top level: BBa_K1855005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466913/range2466913/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466913/range2466913/1
type: Range
persistent identity: range2466913
display id: range2466913
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 192
end: 458
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466916
display id: annotation2466916
version: 1
top level: BBa_K1855005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466916/range2466916/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466916/range2466916/1
type: Range
persistent identity: range2466916
display id: range2466916
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 570
end: 621
orientation: inline

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466909
display id: annotation2466909
version: 1
top level: BBa_K1855005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466909/range2466909/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466909/range2466909/1
type: Range
persistent identity: range2466909
display id: range2466909
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 1
end: 56
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466912
display id: annotation2466912
version: 1
top level: BBa_K1855005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466912/range2466912/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466912/range2466912/1
type: Range
persistent identity: range2466912
display id: range2466912
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 150
end: 185
orientation: inline

BclA
type: SequenceAnnotation
persistent identity: annotation2466911
display id: annotation2466911
version: 1
top level: BBa_K1855005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466911/range2466911/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466911/range2466911/1
type: Range
persistent identity: range2466911
display id: range2466911
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 84
end: 146
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466914
display id: annotation2466914
version: 1
top level: BBa_K1855005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466914/range2466914/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466914/range2466914/1
type: Range
persistent identity: range2466914
display id: range2466914
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 465
end: 488
orientation: inline

BBa_K747093
type: ComponentDefinition
persistent identity: BBa K747093
display id: BBa_K747093
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TC6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 6
sequence: https://synbiohub.org/public/igem/BBa_K747093_sequence/1

TC 6
type: SequenceAnnotation
persistent identity: annotation2197071
display id: annotation2197071
version: 1
top level: BBa_K747093
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747093/annotation2197071/range2197071/1

https://synbiohub.org/public/igem/BBa_K747093/annotation2197071/range2197071/1
type: Range
persistent identity: range2197071
display id: range2197071
version: 1
direction: direction/forward
top level: BBa_K747093
start: 14
end: 211
orientation: inline

BBa_K747047
type: ComponentDefinition
persistent identity: BBa K747047
display id: BBa_K747047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 3
sequence: https://synbiohub.org/public/igem/BBa_K747047_sequence/1

TT 3
type: SequenceAnnotation
persistent identity: annotation2196270
display id: annotation2196270
version: 1
top level: BBa_K747047
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747047/annotation2196270/range2196270/1

https://synbiohub.org/public/igem/BBa_K747047/annotation2196270/range2196270/1
type: Range
persistent identity: range2196270
display id: range2196270
version: 1
direction: direction/forward
top level: BBa_K747047
start: 14
end: 211
orientation: inline

BBa_K747038
type: ComponentDefinition
persistent identity: BBa K747038
display id: BBa_K747038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 3
sequence: https://synbiohub.org/public/igem/BBa_K747038_sequence/1

CG 3
type: SequenceAnnotation
persistent identity: annotation2196220
display id: annotation2196220
version: 1
top level: BBa_K747038
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747038/annotation2196220/range2196220/1

https://synbiohub.org/public/igem/BBa_K747038/annotation2196220/range2196220/1
type: Range
persistent identity: range2196220
display id: range2196220
version: 1
direction: direction/forward
top level: BBa_K747038
start: 14
end: 211
orientation: inline

BBa_K747063
type: ComponentDefinition
persistent identity: BBa K747063
display id: BBa_K747063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 4
sequence: https://synbiohub.org/public/igem/BBa_K747063_sequence/1

TT 4
type: SequenceAnnotation
persistent identity: annotation2197020
display id: annotation2197020
version: 1
top level: BBa_K747063
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747063/annotation2197020/range2197020/1

https://synbiohub.org/public/igem/BBa_K747063/annotation2197020/range2197020/1
type: Range
persistent identity: range2197020
display id: range2197020
version: 1
direction: direction/forward
top level: BBa_K747063
start: 14
end: 211
orientation: inline

BBa_K747050
type: ComponentDefinition
persistent identity: BBa K747050
display id: BBa_K747050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AG4_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AG 4
sequence: https://synbiohub.org/public/igem/BBa_K747050_sequence/1

AG 4
type: SequenceAnnotation
persistent identity: annotation2196279
display id: annotation2196279
version: 1
top level: BBa_K747050
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747050/annotation2196279/range2196279/1

https://synbiohub.org/public/igem/BBa_K747050/annotation2196279/range2196279/1
type: Range
persistent identity: range2196279
display id: range2196279
version: 1
direction: direction/forward
top level: BBa_K747050
start: 14
end: 211
orientation: inline

BBa_K801032
type: ComponentDefinition
persistent identity: BBa K801032
display id: BBa_K801032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GAL4 DNA binding domain
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Gal4 binding domain
mutable notes: -
mutable provenance: extracted from "pGBKT7" from clontech, with RFC25-pre and suffix for protein fusions
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K801032_sequence/1

BBa_K747054
type: ComponentDefinition
persistent identity: BBa K747054
display id: BBa_K747054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 4
sequence: https://synbiohub.org/public/igem/BBa_K747054_sequence/1

CG 4
type: SequenceAnnotation
persistent identity: annotation2196846
display id: annotation2196846
version: 1
top level: BBa_K747054
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747054/annotation2196846/range2196846/1

https://synbiohub.org/public/igem/BBa_K747054/annotation2196846/range2196846/1
type: Range
persistent identity: range2196846
display id: range2196846
version: 1
direction: direction/forward
top level: BBa_K747054
start: 14
end: 211
orientation: inline

BBa_K747078
type: ComponentDefinition
persistent identity: BBa K747078
display id: BBa_K747078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TG 5
sequence: https://synbiohub.org/public/igem/BBa_K747078_sequence/1

TG 5
type: SequenceAnnotation
persistent identity: annotation2197047
display id: annotation2197047
version: 1
top level: BBa_K747078
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747078/annotation2197047/range2197047/1

https://synbiohub.org/public/igem/BBa_K747078/annotation2197047/range2197047/1
type: Range
persistent identity: range2197047
display id: range2197047
version: 1
direction: direction/forward
top level: BBa_K747078
start: 14
end: 211
orientation: inline

BBa_K1861050
type: ComponentDefinition
persistent identity: BBa K1861050
display id: BBa_K1861050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of TALE1 and a SpyTag in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:16:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K392003
component: BBa_K1861101
component: BBa_K1861104
component: BBa_K1861102
component: BBa_K1861501
component: BBa_K1159201
sequence annotation: BBa_K392003
sequence annotation: BBa_K1861102
sequence annotation: BBa_K1159201
sequence annotation: BBa_K1861104
sequence annotation: BBa_K1861101
sequence annotation: BBa_K1861501
sequence: https://synbiohub.org/public/igem/BBa_K1861050_sequence/1

BBa_K392003
type: Component
persistent identity: component2474418
display id: component2474418
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K1861101
type: Component
persistent identity: component2474411
display id: component2474411
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1861104
type: Component
persistent identity: component2474417
display id: component2474417
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1861102
type: Component
persistent identity: component2474412
display id: component2474412
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K1861501
type: Component
persistent identity: component2474413
display id: component2474413
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861501/1

BBa_K1159201
type: Component
persistent identity: component2474416
display id: component2474416
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2474418
display id: annotation2474418
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474418/range2474418/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474418/range2474418/1
type: Range
persistent identity: range2474418
display id: range2474418
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 2525
end: 2653
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2474412
display id: annotation2474412
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474412/range2474412/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474412/range2474412/1
type: Range
persistent identity: range2474412
display id: range2474412
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 239
end: 265
orientation: inline

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2474416
display id: annotation2474416
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474416/range2474416/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474416/range2474416/1
type: Range
persistent identity: range2474416
display id: range2474416
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 2477
end: 2515
orientation: inline

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2474417
display id: annotation2474417
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474417/range2474417/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474417/range2474417/1
type: Range
persistent identity: range2474417
display id: range2474417
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 2516
end: 2524
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2474411
display id: annotation2474411
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474411/range2474411/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474411/range2474411/1
type: Range
persistent identity: range2474411
display id: range2474411
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 1
end: 238
orientation: inline

BBa_K1861501
type: SequenceAnnotation
persistent identity: annotation2474413
display id: annotation2474413
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474413/range2474413/1
component: BBa_K1861501

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474413/range2474413/1
type: Range
persistent identity: range2474413
display id: range2474413
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 266
end: 2476
orientation: inline

BBa_K747071
type: ComponentDefinition
persistent identity: BBa K747071
display id: BBa_K747071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CT5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CT 5
sequence: https://synbiohub.org/public/igem/BBa_K747071_sequence/1

CT 5
type: SequenceAnnotation
persistent identity: annotation2197038
display id: annotation2197038
version: 1
top level: BBa_K747071
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747071/annotation2197038/range2197038/1

https://synbiohub.org/public/igem/BBa_K747071/annotation2197038/range2197038/1
type: Range
persistent identity: range2197038
display id: range2197038
version: 1
direction: direction/forward
top level: BBa_K747071
start: 14
end: 211
orientation: inline

BBa_K747002
type: ComponentDefinition
persistent identity: BBa K747002
display id: BBa_K747002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AG1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AG 1
sequence: https://synbiohub.org/public/igem/BBa_K747002_sequence/1

AG 1
type: SequenceAnnotation
persistent identity: annotation2195763
display id: annotation2195763
version: 1
top level: BBa_K747002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747002/annotation2195763/range2195763/1

https://synbiohub.org/public/igem/BBa_K747002/annotation2195763/range2195763/1
type: Range
persistent identity: range2195763
display id: range2195763
version: 1
direction: direction/forward
top level: BBa_K747002
start: 12
end: 209
orientation: inline

BBa_K747016
type: ComponentDefinition
persistent identity: BBa K747016
display id: BBa_K747016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 2
sequence: https://synbiohub.org/public/igem/BBa_K747016_sequence/1

AA 2
type: SequenceAnnotation
persistent identity: annotation2195965
display id: annotation2195965
version: 1
top level: BBa_K747016
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747016/annotation2195965/range2195965/1

https://synbiohub.org/public/igem/BBa_K747016/annotation2195965/range2195965/1
type: Range
persistent identity: range2195965
display id: range2195965
version: 1
direction: direction/forward
top level: BBa_K747016
start: 14
end: 211
orientation: inline

BBa_K747079
type: ComponentDefinition
persistent identity: BBa K747079
display id: BBa_K747079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 5
sequence: https://synbiohub.org/public/igem/BBa_K747079_sequence/1

TT 5
type: SequenceAnnotation
persistent identity: annotation2197049
display id: annotation2197049
version: 1
top level: BBa_K747079
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747079/annotation2197049/range2197049/1

https://synbiohub.org/public/igem/BBa_K747079/annotation2197049/range2197049/1
type: Range
persistent identity: range2197049
display id: range2197049
version: 1
direction: direction/forward
top level: BBa_K747079
start: 14
end: 211
orientation: inline

BBa_K747075
type: ComponentDefinition
persistent identity: BBa K747075
display id: BBa_K747075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 5
sequence: https://synbiohub.org/public/igem/BBa_K747075_sequence/1

GT 5
type: SequenceAnnotation
persistent identity: annotation2197044
display id: annotation2197044
version: 1
top level: BBa_K747075
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747075/annotation2197044/range2197044/1

https://synbiohub.org/public/igem/BBa_K747075/annotation2197044/range2197044/1
type: Range
persistent identity: range2197044
display id: range2197044
version: 1
direction: direction/forward
top level: BBa_K747075
start: 14
end: 211
orientation: inline

BBa_K801037
type: ComponentDefinition
persistent identity: BBa K801037
display id: BBa_K801037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Linker-GAL4 DNA binding domain
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K365005 BBa_K801032
mutable notes: -
mutable provenance: Composite part of BBa_K365005 BBa_K801032
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K365005
sequence annotation: BBa_K801032
sequence annotation: RFC25 fusion scar
sequence: https://synbiohub.org/public/igem/BBa_K801037_sequence/1

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2198905
display id: annotation2198905
version: 1
top level: BBa_K801037
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801037/annotation2198905/range2198905/1

https://synbiohub.org/public/igem/BBa_K801037/annotation2198905/range2198905/1
type: Range
persistent identity: range2198905
display id: range2198905
version: 1
direction: direction/reverse
top level: BBa_K801037
start: 1
end: 60
orientation: inline

BBa_K801032
type: SequenceAnnotation
persistent identity: annotation2198906
display id: annotation2198906
version: 1
top level: BBa_K801037
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801037/annotation2198906/range2198906/1

https://synbiohub.org/public/igem/BBa_K801037/annotation2198906/range2198906/1
type: Range
persistent identity: range2198906
display id: range2198906
version: 1
direction: direction/reverse
top level: BBa_K801037
start: 67
end: 504
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198908
display id: annotation2198908
version: 1
top level: BBa_K801037
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801037/annotation2198908/range2198908/1

https://synbiohub.org/public/igem/BBa_K801037/annotation2198908/range2198908/1
type: Range
persistent identity: range2198908
display id: range2198908
version: 1
direction: direction/forward
top level: BBa_K801037
start: 61
end: 66
orientation: inline

BBa_K747091
type: ComponentDefinition
persistent identity: BBa K747091
display id: BBa_K747091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 6
sequence: https://synbiohub.org/public/igem/BBa_K747091_sequence/1

GT 6
type: SequenceAnnotation
persistent identity: annotation2197069
display id: annotation2197069
version: 1
top level: BBa_K747091
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747091/annotation2197069/range2197069/1

https://synbiohub.org/public/igem/BBa_K747091/annotation2197069/range2197069/1
type: Range
persistent identity: range2197069
display id: range2197069
version: 1
direction: direction/forward
top level: BBa_K747091
start: 14
end: 211
orientation: inline

BBa_K1861070
type: ComponentDefinition
persistent identity: BBa K1861070
display id: BBa_K1861070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of TALE3 and a SpyTag in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T03:37:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1861701
component: BBa_K392003
component: BBa_K1861101
component: BBa_K1861102
component: BBa_K1159201
component: BBa_K1861104
sequence annotation: BBa_K1861701
sequence annotation: BBa_K1861101
sequence annotation: BBa_K392003
sequence annotation: BBa_K1861104
sequence annotation: BBa_K1159201
sequence annotation: BBa_K1861102
sequence: https://synbiohub.org/public/igem/BBa_K1861070_sequence/1

BBa_K1861701
type: Component
persistent identity: component2474670
display id: component2474670
version: 1
top level: BBa_K1861070
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861701/1

BBa_K392003
type: Component
persistent identity: component2474675
display id: component2474675
version: 1
top level: BBa_K1861070
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K1861101
type: Component
persistent identity: component2474668
display id: component2474668
version: 1
top level: BBa_K1861070
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1861102
type: Component
persistent identity: component2474669
display id: component2474669
version: 1
top level: BBa_K1861070
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K1159201
type: Component
persistent identity: component2474673
display id: component2474673
version: 1
top level: BBa_K1861070
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K1861104
type: Component
persistent identity: component2474674
display id: component2474674
version: 1
top level: BBa_K1861070
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1861701
type: SequenceAnnotation
persistent identity: annotation2474670
display id: annotation2474670
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474670/range2474670/1
component: BBa_K1861701

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474670/range2474670/1
type: Range
persistent identity: range2474670
display id: range2474670
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 266
end: 2480
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2474668
display id: annotation2474668
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474668/range2474668/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474668/range2474668/1
type: Range
persistent identity: range2474668
display id: range2474668
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 1
end: 238
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2474675
display id: annotation2474675
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474675/range2474675/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474675/range2474675/1
type: Range
persistent identity: range2474675
display id: range2474675
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 2529
end: 2657
orientation: inline

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2474674
display id: annotation2474674
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474674/range2474674/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474674/range2474674/1
type: Range
persistent identity: range2474674
display id: range2474674
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 2520
end: 2528
orientation: inline

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2474673
display id: annotation2474673
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474673/range2474673/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474673/range2474673/1
type: Range
persistent identity: range2474673
display id: range2474673
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 2481
end: 2519
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2474669
display id: annotation2474669
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474669/range2474669/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474669/range2474669/1
type: Range
persistent identity: range2474669
display id: range2474669
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 239
end: 265
orientation: inline

BBa_K364001
type: ComponentDefinition
persistent identity: BBa K364001
display id: BBa_K364001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human VDR DBD
creator: Ophir Keret
created: 2010-06-29T11:00:00Z
modified: 2015-05-08T01:12:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _486_
m user id: 0
owner id: 6746
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Human vitamin D receptor dna binding domain
mutable notes: The reconstruction of the DBD to the LBD will be done with RFC 25.
mutable provenance: Human VDR (of the nuclear hormone receptor superfamily)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Zinc Finger (cys2/his2)
sequence: https://synbiohub.org/public/igem/BBa_K364001_sequence/1

Zinc Finger (cys2/his2)
type: SequenceAnnotation
persistent identity: annotation2072067
display id: annotation2072067
version: 1
top level: BBa_K364001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K364001/annotation2072067/range2072067/1

https://synbiohub.org/public/igem/BBa_K364001/annotation2072067/range2072067/1
type: Range
persistent identity: range2072067
display id: range2072067
version: 1
direction: direction/forward
top level: BBa_K364001
start: 40
end: 102
orientation: inline

Zinc Finger (cys2/his2)
type: SequenceAnnotation
persistent identity: annotation2072068
display id: annotation2072068
version: 1
top level: BBa_K364001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K364001/annotation2072068/range2072068/1

https://synbiohub.org/public/igem/BBa_K364001/annotation2072068/range2072068/1
type: Range
persistent identity: range2072068
display id: range2072068
version: 1
direction: direction/forward
top level: BBa_K364001
start: 147
end: 222
orientation: inline

BBa_K747037
type: ComponentDefinition
persistent identity: BBa K747037
display id: BBa_K747037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 3
sequence: https://synbiohub.org/public/igem/BBa_K747037_sequence/1

CC 3
type: SequenceAnnotation
persistent identity: annotation2196216
display id: annotation2196216
version: 1
top level: BBa_K747037
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747037/annotation2196216/range2196216/1

https://synbiohub.org/public/igem/BBa_K747037/annotation2196216/range2196216/1
type: Range
persistent identity: range2196216
display id: range2196216
version: 1
direction: direction/forward
top level: BBa_K747037
start: 14
end: 211
orientation: inline

BBa_K747043
type: ComponentDefinition
persistent identity: BBa K747043
display id: BBa_K747043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 3
sequence: https://synbiohub.org/public/igem/BBa_K747043_sequence/1

GT 3
type: SequenceAnnotation
persistent identity: annotation2196260
display id: annotation2196260
version: 1
top level: BBa_K747043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747043/annotation2196260/range2196260/1

https://synbiohub.org/public/igem/BBa_K747043/annotation2196260/range2196260/1
type: Range
persistent identity: range2196260
display id: range2196260
version: 1
direction: direction/forward
top level: BBa_K747043
start: 14
end: 211
orientation: inline

BBa_K747085
type: ComponentDefinition
persistent identity: BBa K747085
display id: BBa_K747085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 6
sequence: https://synbiohub.org/public/igem/BBa_K747085_sequence/1

CC 6
type: SequenceAnnotation
persistent identity: annotation2197058
display id: annotation2197058
version: 1
top level: BBa_K747085
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747085/annotation2197058/range2197058/1

https://synbiohub.org/public/igem/BBa_K747085/annotation2197058/range2197058/1
type: Range
persistent identity: range2197058
display id: range2197058
version: 1
direction: direction/forward
top level: BBa_K747085
start: 14
end: 211
orientation: inline

BBa_K747006
type: ComponentDefinition
persistent identity: BBa K747006
display id: BBa_K747006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 1
sequence: https://synbiohub.org/public/igem/BBa_K747006_sequence/1

CG 1
type: SequenceAnnotation
persistent identity: annotation2195805
display id: annotation2195805
version: 1
top level: BBa_K747006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747006/annotation2195805/range2195805/1

https://synbiohub.org/public/igem/BBa_K747006/annotation2195805/range2195805/1
type: Range
persistent identity: range2195805
display id: range2195805
version: 1
direction: direction/forward
top level: BBa_K747006
start: 12
end: 209
orientation: inline

BBa_K747025
type: ComponentDefinition
persistent identity: BBa K747025
display id: BBa_K747025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 2
sequence: https://synbiohub.org/public/igem/BBa_K747025_sequence/1

GC 2
type: SequenceAnnotation
persistent identity: annotation2196083
display id: annotation2196083
version: 1
top level: BBa_K747025
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747025/annotation2196083/range2196083/1

https://synbiohub.org/public/igem/BBa_K747025/annotation2196083/range2196083/1
type: Range
persistent identity: range2196083
display id: range2196083
version: 1
direction: direction/forward
top level: BBa_K747025
start: 14
end: 211
orientation: inline

BBa_K747059
type: ComponentDefinition
persistent identity: BBa K747059
display id: BBa_K747059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 4
sequence: https://synbiohub.org/public/igem/BBa_K747059_sequence/1

GT 4
type: SequenceAnnotation
persistent identity: annotation2197017
display id: annotation2197017
version: 1
top level: BBa_K747059
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747059/annotation2197017/range2197017/1

https://synbiohub.org/public/igem/BBa_K747059/annotation2197017/range2197017/1
type: Range
persistent identity: range2197017
display id: range2197017
version: 1
direction: direction/forward
top level: BBa_K747059
start: 14
end: 211
orientation: inline

BBa_K747033
type: ComponentDefinition
persistent identity: BBa K747033
display id: BBa_K747033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AC3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 3
sequence: https://synbiohub.org/public/igem/BBa_K747033_sequence/1

AT 3
type: SequenceAnnotation
persistent identity: annotation2196208
display id: annotation2196208
version: 1
top level: BBa_K747033
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747033/annotation2196208/range2196208/1

https://synbiohub.org/public/igem/BBa_K747033/annotation2196208/range2196208/1
type: Range
persistent identity: range2196208
display id: range2196208
version: 1
direction: direction/forward
top level: BBa_K747033
start: 14
end: 211
orientation: inline

BBa_K165008
type: ComponentDefinition
persistent identity: BBa K165008
display id: BBa_K165008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YY1 DNA-binding domain
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K165008_sequence/1

BBa_K747011
type: ComponentDefinition
persistent identity: BBa K747011
display id: BBa_K747011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GT1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GT 1
sequence: https://synbiohub.org/public/igem/BBa_K747011_sequence/1

GT 1
type: SequenceAnnotation
persistent identity: annotation2195848
display id: annotation2195848
version: 1
top level: BBa_K747011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747011/annotation2195848/range2195848/1

https://synbiohub.org/public/igem/BBa_K747011/annotation2195848/range2195848/1
type: Range
persistent identity: range2195848
display id: range2195848
version: 1
direction: direction/forward
top level: BBa_K747011
start: 12
end: 209
orientation: inline

BBa_K747092
type: ComponentDefinition
persistent identity: BBa K747092
display id: BBa_K747092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 6
sequence: https://synbiohub.org/public/igem/BBa_K747092_sequence/1

TA 6
type: SequenceAnnotation
persistent identity: annotation2197070
display id: annotation2197070
version: 1
top level: BBa_K747092
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747092/annotation2197070/range2197070/1

https://synbiohub.org/public/igem/BBa_K747092/annotation2197070/range2197070/1
type: Range
persistent identity: range2197070
display id: range2197070
version: 1
direction: direction/forward
top level: BBa_K747092
start: 14
end: 211
orientation: inline

BBa_K747030
type: ComponentDefinition
persistent identity: BBa K747030
display id: BBa_K747030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TG 2
sequence: https://synbiohub.org/public/igem/BBa_K747030_sequence/1

TG 2
type: SequenceAnnotation
persistent identity: annotation2196204
display id: annotation2196204
version: 1
top level: BBa_K747030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747030/annotation2196204/range2196204/1

https://synbiohub.org/public/igem/BBa_K747030/annotation2196204/range2196204/1
type: Range
persistent identity: range2196204
display id: range2196204
version: 1
direction: direction/forward
top level: BBa_K747030
start: 14
end: 211
orientation: inline

BBa_K747049
type: ComponentDefinition
persistent identity: BBa K747049
display id: BBa_K747049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AC4_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC 4
sequence: https://synbiohub.org/public/igem/BBa_K747049_sequence/1

AC 4
type: SequenceAnnotation
persistent identity: annotation2196276
display id: annotation2196276
version: 1
top level: BBa_K747049
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747049/annotation2196276/range2196276/1

https://synbiohub.org/public/igem/BBa_K747049/annotation2196276/range2196276/1
type: Range
persistent identity: range2196276
display id: range2196276
version: 1
direction: direction/forward
top level: BBa_K747049
start: 14
end: 211
orientation: inline

BBa_K747082
type: ComponentDefinition
persistent identity: BBa K747082
display id: BBa_K747082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AG6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC 6
sequence: https://synbiohub.org/public/igem/BBa_K747082_sequence/1

AC 6
type: SequenceAnnotation
persistent identity: annotation2197055
display id: annotation2197055
version: 1
top level: BBa_K747082
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747082/annotation2197055/range2197055/1

https://synbiohub.org/public/igem/BBa_K747082/annotation2197055/range2197055/1
type: Range
persistent identity: range2197055
display id: range2197055
version: 1
direction: direction/forward
top level: BBa_K747082
start: 14
end: 211
orientation: inline

BBa_K801041
type: ComponentDefinition
persistent identity: BBa K801041
display id: BBa_K801041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-PhyB-linker-LexA (part for a LexA based light-switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801033
mutable notes: -
mutable provenance: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801033
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Essential Gly for photoconvertible binding of PIF3
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin (PCB)
sequence annotation: BBa_K365005
sequence annotation: RFC25 fusion scar
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: BBa_K801033
sequence annotation: BBa_K801030
sequence annotation: BBa_K801031
sequence annotation: Essential Arg for photoconvertible binding of PIF3
sequence annotation: BBa_K801036
sequence: https://synbiohub.org/public/igem/BBa_K801041_sequence/1

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199711
display id: annotation2199711
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199711/range2199711/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199711/range2199711/1
type: Range
persistent identity: range2199711
display id: range2199711
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1102
end: 1104
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin (PCB)
type: SequenceAnnotation
persistent identity: annotation2199713
display id: annotation2199713
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199713/range2199713/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199713/range2199713/1
type: Range
persistent identity: range2199713
display id: range2199713
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1129
end: 1131
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2199715
display id: annotation2199715
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199715/range2199715/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199715/range2199715/1
type: Range
persistent identity: range2199715
display id: range2199715
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2791
end: 2851
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199722
display id: annotation2199722
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199722/range2199722/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199722/range2199722/1
type: Range
persistent identity: range2199722
display id: range2199722
version: 1
direction: direction/forward
top level: BBa_K801041
start: 58
end: 63
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199707
display id: annotation2199707
version: 1
top level: BBa_K801041
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199707/range2199707/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199707/range2199707/1
type: Range
persistent identity: range2199707
display id: range2199707
version: 1
direction: direction/forward
top level: BBa_K801041
start: 22
end: 42
orientation: inline

BBa_K801033
type: SequenceAnnotation
persistent identity: annotation2199717
display id: annotation2199717
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199717/range2199717/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199717/range2199717/1
type: Range
persistent identity: range2199717
display id: range2199717
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2857
end: 3459
orientation: inline

BBa_K801030
type: SequenceAnnotation
persistent identity: annotation2199708
display id: annotation2199708
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199708/range2199708/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199708/range2199708/1
type: Range
persistent identity: range2199708
display id: range2199708
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1
end: 57
orientation: inline

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2199714
display id: annotation2199714
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199714/range2199714/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199714/range2199714/1
type: Range
persistent identity: range2199714
display id: range2199714
version: 1
direction: direction/forward
top level: BBa_K801041
start: 64
end: 2784
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199716
display id: annotation2199716
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199716/range2199716/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199716/range2199716/1
type: Range
persistent identity: range2199716
display id: range2199716
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2851
end: 2856
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199709
display id: annotation2199709
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199709/range2199709/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199709/range2199709/1
type: Range
persistent identity: range2199709
display id: range2199709
version: 1
direction: direction/forward
top level: BBa_K801041
start: 388
end: 390
orientation: inline

BBa_K801036
type: SequenceAnnotation
persistent identity: annotation2199718
display id: annotation2199718
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199718/range2199718/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199718/range2199718/1
type: Range
persistent identity: range2199718
display id: range2199718
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2791
end: 3459
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199724
display id: annotation2199724
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199724/range2199724/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199724/range2199724/1
type: Range
persistent identity: range2199724
display id: range2199724
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2785
end: 2790
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199710
display id: annotation2199710
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199710/range2199710/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199710/range2199710/1
type: Range
persistent identity: range2199710
display id: range2199710
version: 1
direction: direction/forward
top level: BBa_K801041
start: 391
end: 393
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199712
display id: annotation2199712
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199712/range2199712/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199712/range2199712/1
type: Range
persistent identity: range2199712
display id: range2199712
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1114
end: 1116
orientation: inline

BBa_K747081
type: ComponentDefinition
persistent identity: BBa K747081
display id: BBa_K747081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AC6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC 5
sequence: https://synbiohub.org/public/igem/BBa_K747081_sequence/1

AC 5
type: SequenceAnnotation
persistent identity: annotation2197053
display id: annotation2197053
version: 1
top level: BBa_K747081
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747081/annotation2197053/range2197053/1

https://synbiohub.org/public/igem/BBa_K747081/annotation2197053/range2197053/1
type: Range
persistent identity: range2197053
display id: range2197053
version: 1
direction: direction/forward
top level: BBa_K747081
start: 14
end: 211
orientation: inline

BBa_K1855002
type: ComponentDefinition
persistent identity: BBa K1855002
display id: BBa_K1855002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lpp-OmpA sZF10
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:11:52Z
discontinued: false
dominant: false
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (sZF10),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to TGCTGCTTC on the cell surface of E.coli.
mutable notes: Lpp-OmpA is originally from E.coli; sZF10 is from http://www.nature.com/nmeth/journal/v10/n5/abs/nmeth.2407.html
mutable provenance: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The Lpp-OmpA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The sZF10 domain is preceeded by an NheI cut site and followed by a ClaI cut site.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 3x FLAG
sequence annotation: B0034 Strong RBS
sequence annotation: BBa_K864600 T0 Terminator
sequence annotation: Lpp-OmpA
sequence annotation: Linker
sequence annotation: pL_lac
sequence annotation: sZF10
sequence: https://synbiohub.org/public/igem/BBa_K1855002_sequence/1

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466785
display id: annotation2466785
version: 1
top level: BBa_K1855002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466785/range2466785/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466785/range2466785/1
type: Range
persistent identity: range2466785
display id: range2466785
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 867
end: 938
orientation: inline

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466777
display id: annotation2466777
version: 1
top level: BBa_K1855002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466777/range2466777/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466777/range2466777/1
type: Range
persistent identity: range2466777
display id: range2466777
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 60
end: 80
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466787
display id: annotation2466787
version: 1
top level: BBa_K1855002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466787/range2466787/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466787/range2466787/1
type: Range
persistent identity: range2466787
display id: range2466787
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 945
end: 996
orientation: inline

Lpp-OmpA
type: SequenceAnnotation
persistent identity: annotation2466779
display id: annotation2466779
version: 1
top level: BBa_K1855002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466779/range2466779/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466779/range2466779/1
type: Range
persistent identity: range2466779
display id: range2466779
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 84
end: 518
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466784
display id: annotation2466784
version: 1
top level: BBa_K1855002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466784/range2466784/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466784/range2466784/1
type: Range
persistent identity: range2466784
display id: range2466784
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 840
end: 863
orientation: inline

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466776
display id: annotation2466776
version: 1
top level: BBa_K1855002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466776/range2466776/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466776/range2466776/1
type: Range
persistent identity: range2466776
display id: range2466776
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 1
end: 56
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466781
display id: annotation2466781
version: 1
top level: BBa_K1855002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466781/range2466781/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466781/range2466781/1
type: Range
persistent identity: range2466781
display id: range2466781
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 522
end: 557
orientation: inline

sZF10
type: SequenceAnnotation
persistent identity: annotation2466782
display id: annotation2466782
version: 1
top level: BBa_K1855002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466782/range2466782/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466782/range2466782/1
type: Range
persistent identity: range2466782
display id: range2466782
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 564
end: 833
orientation: inline

BBa_K801040
type: ComponentDefinition
persistent identity: BBa K801040
display id: BBa_K801040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-PhyB-linker-Gal4DBD (part for GAL4 based light switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801035
mutable notes: -
mutable provenance: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801035
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K801032
sequence annotation: Essential Arg for photoconvertible binding of PIF3
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: Essential Gly for photoconvertible binding of PIF3
sequence annotation: BBa_K801037
sequence annotation: BBa_K801031
sequence annotation: BBa_K801038
sequence annotation: BBa_K365005
sequence annotation: RFC25 fusion scar
sequence annotation: BBa_K801030
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin (PCB)
sequence: https://synbiohub.org/public/igem/BBa_K801040_sequence/1

BBa_K801032
type: SequenceAnnotation
persistent identity: annotation2199590
display id: annotation2199590
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199590/range2199590/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199590/range2199590/1
type: Range
persistent identity: range2199590
display id: range2199590
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2857
end: 3294
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199584
display id: annotation2199584
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199584/range2199584/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199584/range2199584/1
type: Range
persistent identity: range2199584
display id: range2199584
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1114
end: 1116
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199578
display id: annotation2199578
version: 1
top level: BBa_K801040
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199578/range2199578/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199578/range2199578/1
type: Range
persistent identity: range2199578
display id: range2199578
version: 1
direction: direction/forward
top level: BBa_K801040
start: 22
end: 42
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199583
display id: annotation2199583
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199583/range2199583/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199583/range2199583/1
type: Range
persistent identity: range2199583
display id: range2199583
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1102
end: 1104
orientation: inline

BBa_K801037
type: SequenceAnnotation
persistent identity: annotation2199588
display id: annotation2199588
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199588/range2199588/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199588/range2199588/1
type: Range
persistent identity: range2199588
display id: range2199588
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2791
end: 3294
orientation: inline

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2199580
display id: annotation2199580
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199580/range2199580/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199580/range2199580/1
type: Range
persistent identity: range2199580
display id: range2199580
version: 1
direction: direction/forward
top level: BBa_K801040
start: 64
end: 2784
orientation: inline

BBa_K801038
type: SequenceAnnotation
persistent identity: annotation2199591
display id: annotation2199591
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199591/range2199591/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199591/range2199591/1
type: Range
persistent identity: range2199591
display id: range2199591
version: 1
direction: direction/forward
top level: BBa_K801040
start: 64
end: 3294
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2199587
display id: annotation2199587
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199587/range2199587/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199587/range2199587/1
type: Range
persistent identity: range2199587
display id: range2199587
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2791
end: 2850
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199589
display id: annotation2199589
version: 1
top level: BBa_K801040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199589/range2199589/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199589/range2199589/1
type: Range
persistent identity: range2199589
display id: range2199589
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2851
end: 2856
orientation: inline

BBa_K801030
type: SequenceAnnotation
persistent identity: annotation2199579
display id: annotation2199579
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199579/range2199579/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199579/range2199579/1
type: Range
persistent identity: range2199579
display id: range2199579
version: 1
direction: direction/reverse
top level: BBa_K801040
start: 1
end: 57
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199582
display id: annotation2199582
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199582/range2199582/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199582/range2199582/1
type: Range
persistent identity: range2199582
display id: range2199582
version: 1
direction: direction/forward
top level: BBa_K801040
start: 391
end: 393
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199586
display id: annotation2199586
version: 1
top level: BBa_K801040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199586/range2199586/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199586/range2199586/1
type: Range
persistent identity: range2199586
display id: range2199586
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2785
end: 2790
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin (PCB)
type: SequenceAnnotation
persistent identity: annotation2199585
display id: annotation2199585
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199585/range2199585/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199585/range2199585/1
type: Range
persistent identity: range2199585
display id: range2199585
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1129
end: 1131
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199581
display id: annotation2199581
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199581/range2199581/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199581/range2199581/1
type: Range
persistent identity: range2199581
display id: range2199581
version: 1
direction: direction/forward
top level: BBa_K801040
start: 388
end: 390
orientation: inline

BBa_K747048
type: ComponentDefinition
persistent identity: BBa K747048
display id: BBa_K747048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA4_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 4
sequence: https://synbiohub.org/public/igem/BBa_K747048_sequence/1

AA 4
type: SequenceAnnotation
persistent identity: annotation2196273
display id: annotation2196273
version: 1
top level: BBa_K747048
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747048/annotation2196273/range2196273/1

https://synbiohub.org/public/igem/BBa_K747048/annotation2196273/range2196273/1
type: Range
persistent identity: range2196273
display id: range2196273
version: 1
direction: direction/forward
top level: BBa_K747048
start: 14
end: 211
orientation: inline

lexA DBD
type: ComponentDefinition
persistent identity: BBa K165009
display id: BBa_K165009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA DNA-binding domain
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K165009_sequence/1

BBa_K747060
type: ComponentDefinition
persistent identity: BBa K747060
display id: BBa_K747060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 4
sequence: https://synbiohub.org/public/igem/BBa_K747060_sequence/1

TA 4
type: SequenceAnnotation
persistent identity: annotation2197016
display id: annotation2197016
version: 1
top level: BBa_K747060
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747060/annotation2197016/range2197016/1

https://synbiohub.org/public/igem/BBa_K747060/annotation2197016/range2197016/1
type: Range
persistent identity: range2197016
display id: range2197016
version: 1
direction: direction/forward
top level: BBa_K747060
start: 14
end: 211
orientation: inline

BBa_K747022
type: ComponentDefinition
persistent identity: BBa K747022
display id: BBa_K747022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 2
sequence: https://synbiohub.org/public/igem/BBa_K747022_sequence/1

CG 2
type: SequenceAnnotation
persistent identity: annotation2196045
display id: annotation2196045
version: 1
top level: BBa_K747022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747022/annotation2196045/range2196045/1

https://synbiohub.org/public/igem/BBa_K747022/annotation2196045/range2196045/1
type: Range
persistent identity: range2196045
display id: range2196045
version: 1
direction: direction/forward
top level: BBa_K747022
start: 14
end: 211
orientation: inline

BBa_K747024
type: ComponentDefinition
persistent identity: BBa K747024
display id: BBa_K747024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 2
sequence: https://synbiohub.org/public/igem/BBa_K747024_sequence/1

GA 2
type: SequenceAnnotation
persistent identity: annotation2196062
display id: annotation2196062
version: 1
top level: BBa_K747024
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747024/annotation2196062/range2196062/1

https://synbiohub.org/public/igem/BBa_K747024/annotation2196062/range2196062/1
type: Range
persistent identity: range2196062
display id: range2196062
version: 1
direction: direction/forward
top level: BBa_K747024
start: 14
end: 211
orientation: inline

BBa_K747017
type: ComponentDefinition
persistent identity: BBa K747017
display id: BBa_K747017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AC2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC 2
sequence: https://synbiohub.org/public/igem/BBa_K747017_sequence/1

AC 2
type: SequenceAnnotation
persistent identity: annotation2195966
display id: annotation2195966
version: 1
top level: BBa_K747017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747017/annotation2195966/range2195966/1

https://synbiohub.org/public/igem/BBa_K747017/annotation2195966/range2195966/1
type: Range
persistent identity: range2195966
display id: range2195966
version: 1
direction: direction/forward
top level: BBa_K747017
start: 14
end: 211
orientation: inline

BBa_K747044
type: ComponentDefinition
persistent identity: BBa K747044
display id: BBa_K747044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 3
sequence: https://synbiohub.org/public/igem/BBa_K747044_sequence/1

TA 3
type: SequenceAnnotation
persistent identity: annotation2196261
display id: annotation2196261
version: 1
top level: BBa_K747044
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747044/annotation2196261/range2196261/1

https://synbiohub.org/public/igem/BBa_K747044/annotation2196261/range2196261/1
type: Range
persistent identity: range2196261
display id: range2196261
version: 1
direction: direction/forward
top level: BBa_K747044
start: 14
end: 211
orientation: inline

BBa_K747095
type: ComponentDefinition
persistent identity: BBa K747095
display id: BBa_K747095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 6
sequence: https://synbiohub.org/public/igem/BBa_K747095_sequence/1

TT 6
type: SequenceAnnotation
persistent identity: annotation2197073
display id: annotation2197073
version: 1
top level: BBa_K747095
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747095/annotation2197073/range2197073/1

https://synbiohub.org/public/igem/BBa_K747095/annotation2197073/range2197073/1
type: Range
persistent identity: range2197073
display id: range2197073
version: 1
direction: direction/forward
top level: BBa_K747095
start: 14
end: 211
orientation: inline

BBa_K801043
type: ComponentDefinition
persistent identity: BBa K801043
display id: BBa_K801043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA based yeast light-swithable promoter system
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: composite part of Bba_K319003, K801039, BBa_K801011 , Bba_K319003, Bba_K801041, and BBa_K801011
mutable notes: -
mutable provenance: composite part of Bba_K319003, K801039, BBa_K801011 , Bba_K319003, Bba_K801041, and BBa_K801011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
sequence annotation: BBa_K319003 (pTEF1, TEF1 promoter)
sequence annotation: Linker
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: BBa_K801011, (tTEF1, TEF1 terminator)
sequence annotation: SV40 NLS (SV40 nuclear localization signal)
sequence annotation: BBa_K365005 (20 amino acids long linker)
sequence annotation: Phytochrome B (PhyB, 908 first N-terminal amino acids)
sequence annotation: LexA
sequence annotation: T7 promoter (for sequencing)
sequence annotation: BBa_K801041 (SV40NLS-PhyB-Linker-LexA)
sequence annotation: HA epitope tage
sequence annotation: GAL4AD (GAL4 transcriptonal activation domain)
sequence annotation: SV40NLS-PhyB-Linker-LexA
sequence: https://synbiohub.org/public/igem/BBa_K801043_sequence/1

BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
type: SequenceAnnotation
persistent identity: annotation2198798
display id: annotation2198798
version: 1
top level: BBa_K801043
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198798/range2198798/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198798/range2198798/1
type: Range
persistent identity: range2198798
display id: range2198798
version: 1
direction: direction/forward
top level: BBa_K801043
start: 456
end: 1259
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198797
display id: annotation2198797
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198797/range2198797/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198797/range2198797/1
type: Range
persistent identity: range2198797
display id: range2198797
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1
end: 450
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2198801
display id: annotation2198801
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198801/range2198801/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198801/range2198801/1
type: Range
persistent identity: range2198801
display id: range2198801
version: 1
direction: direction/forward
top level: BBa_K801043
start: 864
end: 959
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198806
display id: annotation2198806
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198806/range2198806/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198806/range2198806/1
type: Range
persistent identity: range2198806
display id: range2198806
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1782
end: 2231
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198804
display id: annotation2198804
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198804/range2198804/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198804/range2198804/1
type: Range
persistent identity: range2198804
display id: range2198804
version: 1
direction: direction/forward
top level: BBa_K801043
start: 960
end: 1256
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198812
display id: annotation2198812
version: 1
top level: BBa_K801043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198812/range2198812/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198812/range2198812/1
type: Range
persistent identity: range2198812
display id: range2198812
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5722
end: 6228
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198799
display id: annotation2198799
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198799/range2198799/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198799/range2198799/1
type: Range
persistent identity: range2198799
display id: range2198799
version: 1
direction: direction/forward
top level: BBa_K801043
start: 465
end: 521
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198805
display id: annotation2198805
version: 1
top level: BBa_K801043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198805/range2198805/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198805/range2198805/1
type: Range
persistent identity: range2198805
display id: range2198805
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1268
end: 1774
orientation: inline

BBa_K365005 (20 amino acids long linker)
type: SequenceAnnotation
persistent identity: annotation2198810
display id: annotation2198810
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198810/range2198810/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198810/range2198810/1
type: Range
persistent identity: range2198810
display id: range2198810
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5036
end: 5095
orientation: inline

Phytochrome B (PhyB, 908 first N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198809
display id: annotation2198809
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198809/range2198809/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198809/range2198809/1
type: Range
persistent identity: range2198809
display id: range2198809
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2309
end: 5029
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation2198811
display id: annotation2198811
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198811/range2198811/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198811/range2198811/1
type: Range
persistent identity: range2198811
display id: range2198811
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5102
end: 5704
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2198802
display id: annotation2198802
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198802/range2198802/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198802/range2198802/1
type: Range
persistent identity: range2198802
display id: range2198802
version: 1
direction: direction/forward
top level: BBa_K801043
start: 869
end: 891
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198808
display id: annotation2198808
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198808/range2198808/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198808/range2198808/1
type: Range
persistent identity: range2198808
display id: range2198808
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2246
end: 2302
orientation: inline

BBa_K801041 (SV40NLS-PhyB-Linker-LexA)
type: SequenceAnnotation
persistent identity: annotation2198814
display id: annotation2198814
version: 1
top level: BBa_K801043
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198814/range2198814/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198814/range2198814/1
type: Range
persistent identity: range2198814
display id: range2198814
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2246
end: 5704
orientation: inline

HA epitope tage
type: SequenceAnnotation
persistent identity: annotation2198803
display id: annotation2198803
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198803/range2198803/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198803/range2198803/1
type: Range
persistent identity: range2198803
display id: range2198803
version: 1
direction: direction/forward
top level: BBa_K801043
start: 912
end: 932
orientation: inline

GAL4AD (GAL4 transcriptonal activation domain)
type: SequenceAnnotation
persistent identity: annotation2198800
display id: annotation2198800
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198800/range2198800/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198800/range2198800/1
type: Range
persistent identity: range2198800
display id: range2198800
version: 1
direction: direction/forward
top level: BBa_K801043
start: 525
end: 863
orientation: inline

SV40NLS-PhyB-Linker-LexA
type: SequenceAnnotation
persistent identity: annotation2198807
display id: annotation2198807
version: 1
top level: BBa_K801043
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198807/range2198807/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198807/range2198807/1
type: Range
persistent identity: range2198807
display id: range2198807
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2237
end: 5713
orientation: inline

BBa_K747057
type: ComponentDefinition
persistent identity: BBa K747057
display id: BBa_K747057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 4
sequence: https://synbiohub.org/public/igem/BBa_K747057_sequence/1

GC 4
type: SequenceAnnotation
persistent identity: annotation2197014
display id: annotation2197014
version: 1
top level: BBa_K747057
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747057/annotation2197014/range2197014/1

https://synbiohub.org/public/igem/BBa_K747057/annotation2197014/range2197014/1
type: Range
persistent identity: range2197014
display id: range2197014
version: 1
direction: direction/forward
top level: BBa_K747057
start: 14
end: 211
orientation: inline

BBa_K747028
type: ComponentDefinition
persistent identity: BBa K747028
display id: BBa_K747028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TA2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TA 2
sequence: https://synbiohub.org/public/igem/BBa_K747028_sequence/1

TA 2
type: SequenceAnnotation
persistent identity: annotation2196199
display id: annotation2196199
version: 1
top level: BBa_K747028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747028/annotation2196199/range2196199/1

https://synbiohub.org/public/igem/BBa_K747028/annotation2196199/range2196199/1
type: Range
persistent identity: range2196199
display id: range2196199
version: 1
direction: direction/forward
top level: BBa_K747028
start: 14
end: 211
orientation: inline

BBa_K618112
type: ComponentDefinition
persistent identity: BBa K618112
display id: BBa_K618112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD ompA-zif268
creator: Chris Bate, Ben Park, Marc Ammerlaan
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _790_
m user id: 0
owner id: 8977
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a fusion between ompA and the DNA binding protein zif268 under the control of pBAD. The protein should be present on the outer surface of cells and bind the zif268 recognition sequence (GATGCTGCA). This will also allow cells to be anchored to a glass slide with the appropriate DNA attached. This can serve as a way to position cells in any desired pattern.
mutable notes: NOTE- the sequence as listed here is wrong. Because part K165006 is supplied on a Silver lab vector (Bba_J63010) with a modified biobrick prefix, the scar between ompA and zif268 is non-standard. The G at position 840 is not there. This has been verified by sequencing. Deleting the G keeps zif268 in frame with ompA. Part length is 1364bp. The sequence was generated automatically and I couldn't figure out how to edit it.
mutable provenance: parts come from iGEM distribution and from iGEM HQ
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K165006
component: BBa_K103006
component: BBa_K259007
sequence annotation: BBa_K103006
sequence annotation: BBa_K165006
sequence annotation: BBa_K259007
sequence: https://synbiohub.org/public/igem/BBa_K618112_sequence/1

BBa_K165006
type: Component
persistent identity: component2144956
display id: component2144956
version: 1
top level: BBa_K618112
access: public
definition: BBa_K165006

BBa_K103006
type: Component
persistent identity: component2144955
display id: component2144955
version: 1
top level: BBa_K618112
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_K259007
type: Component
persistent identity: component2144949
display id: component2144949
version: 1
top level: BBa_K618112
access: public
definition: https://synbiohub.org/public/igem/BBa_K259007/1

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation2144955
display id: annotation2144955
version: 1
top level: BBa_K618112
location: https://synbiohub.org/public/igem/BBa_K618112/annotation2144955/range2144955/1
component: BBa_K103006

https://synbiohub.org/public/igem/BBa_K618112/annotation2144955/range2144955/1
type: Range
persistent identity: range2144955
display id: range2144955
version: 1
direction: direction/reverse
top level: BBa_K618112
start: 369
end: 832
orientation: inline

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation2144956
display id: annotation2144956
version: 1
top level: BBa_K618112
location: https://synbiohub.org/public/igem/BBa_K618112/annotation2144956/range2144956/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K618112/annotation2144956/range2144956/1
type: Range
persistent identity: range2144956
display id: range2144956
version: 1
direction: direction/reverse
top level: BBa_K618112
start: 841
end: 1092
orientation: inline

BBa_K259007
type: SequenceAnnotation
persistent identity: annotation2144949
display id: annotation2144949
version: 1
top level: BBa_K618112
location: https://synbiohub.org/public/igem/BBa_K618112/annotation2144949/range2144949/1
component: BBa_K259007

https://synbiohub.org/public/igem/BBa_K618112/annotation2144949/range2144949/1
type: Range
persistent identity: range2144949
display id: range2144949
version: 1
direction: direction/reverse
top level: BBa_K618112
start: 1
end: 360
orientation: inline

BBa_K747062
type: ComponentDefinition
persistent identity: BBa K747062
display id: BBa_K747062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TG 4
sequence: https://synbiohub.org/public/igem/BBa_K747062_sequence/1

TG 4
type: SequenceAnnotation
persistent identity: annotation2197019
display id: annotation2197019
version: 1
top level: BBa_K747062
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747062/annotation2197019/range2197019/1

https://synbiohub.org/public/igem/BBa_K747062/annotation2197019/range2197019/1
type: Range
persistent identity: range2197019
display id: range2197019
version: 1
direction: direction/forward
top level: BBa_K747062
start: 14
end: 211
orientation: inline

BBa_K801033
type: ComponentDefinition
persistent identity: BBa K801033
display id: BBa_K801033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA DNA binding protein
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: LexA protein, improved version of BBa_K105005 to allow N- and C-terminal protein fusions using RFC25
mutable notes: -
mutable provenance: improved version of BBa_K105005 to allow N- and C-terminal protein fusions using RFC25
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K801033_sequence/1

BBa_K105007
type: ComponentDefinition
persistent identity: BBa K105007
display id: BBa_K105007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gal4 - DNA binding domain
creator: Katja Karstens
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Gal4 is a yeast transcription factor which activates the transcription.<br\n> This BioBrick is the DNA binding domain of Gal4, which binds specificly to the regulatory sequence BBa_K105024.
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: lab plasmid
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K105007_sequence/1

BBa_K747067
type: ComponentDefinition
persistent identity: BBa K747067
display id: BBa_K747067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 5
sequence: https://synbiohub.org/public/igem/BBa_K747067_sequence/1

AT 5
type: SequenceAnnotation
persistent identity: annotation2197030
display id: annotation2197030
version: 1
top level: BBa_K747067
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747067/annotation2197030/range2197030/1

https://synbiohub.org/public/igem/BBa_K747067/annotation2197030/range2197030/1
type: Range
persistent identity: range2197030
display id: range2197030
version: 1
direction: direction/forward
top level: BBa_K747067
start: 14
end: 211
orientation: inline

BBa_K747065
type: ComponentDefinition
persistent identity: BBa K747065
display id: BBa_K747065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AC5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AC 5
sequence: https://synbiohub.org/public/igem/BBa_K747065_sequence/1

AC 5
type: SequenceAnnotation
persistent identity: annotation2197022
display id: annotation2197022
version: 1
top level: BBa_K747065
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747065/annotation2197022/range2197022/1

https://synbiohub.org/public/igem/BBa_K747065/annotation2197022/range2197022/1
type: Range
persistent identity: range2197022
display id: range2197022
version: 1
direction: direction/forward
top level: BBa_K747065
start: 14
end: 211
orientation: inline

BBa_K364101
type: ComponentDefinition
persistent identity: BBa K364101
display id: BBa_K364101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zinc Finger Protein AAA
creator: Ophir Keret
created: 2010-07-26T11:00:00Z
modified: 2015-05-08T01:12:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _486_
m user id: 0
owner id: 6746
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part codes for a Cys2His2 zinc finger protein to be used in assembly of zinc finger polymers (to be continued).
mutable notes: Special RFC needs to be constructed for this purpose
mutable provenance: Completely artificial
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: Recognition Alpha Helix
sequence: https://synbiohub.org/public/igem/BBa_K364101_sequence/1

Recognition Alpha Helix
type: SequenceAnnotation
persistent identity: annotation2077781
display id: annotation2077781
version: 1
top level: BBa_K364101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K364101/annotation2077781/range2077781/1

https://synbiohub.org/public/igem/BBa_K364101/annotation2077781/range2077781/1
type: Range
persistent identity: range2077781
display id: range2077781
version: 1
direction: direction/forward
top level: BBa_K364101
start: 34
end: 54
orientation: inline

BBa_K747064
type: ComponentDefinition
persistent identity: BBa K747064
display id: BBa_K747064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 5
sequence: https://synbiohub.org/public/igem/BBa_K747064_sequence/1

AA 5
type: SequenceAnnotation
persistent identity: annotation2197021
display id: annotation2197021
version: 1
top level: BBa_K747064
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747064/annotation2197021/range2197021/1

https://synbiohub.org/public/igem/BBa_K747064/annotation2197021/range2197021/1
type: Range
persistent identity: range2197021
display id: range2197021
version: 1
direction: direction/forward
top level: BBa_K747064
start: 12
end: 211
orientation: inline

BBa_K747021
type: ComponentDefinition
persistent identity: BBa K747021
display id: BBa_K747021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 2
sequence: https://synbiohub.org/public/igem/BBa_K747021_sequence/1

CC 2
type: SequenceAnnotation
persistent identity: annotation2196025
display id: annotation2196025
version: 1
top level: BBa_K747021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747021/annotation2196025/range2196025/1

https://synbiohub.org/public/igem/BBa_K747021/annotation2196025/range2196025/1
type: Range
persistent identity: range2196025
display id: range2196025
version: 1
direction: direction/forward
top level: BBa_K747021
start: 14
end: 211
orientation: inline

BBa_K747053
type: ComponentDefinition
persistent identity: BBa K747053
display id: BBa_K747053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 4
sequence: https://synbiohub.org/public/igem/BBa_K747053_sequence/1

CC 4
type: SequenceAnnotation
persistent identity: annotation2196693
display id: annotation2196693
version: 1
top level: BBa_K747053
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747053/annotation2196693/range2196693/1

https://synbiohub.org/public/igem/BBa_K747053/annotation2196693/range2196693/1
type: Range
persistent identity: range2196693
display id: range2196693
version: 1
direction: direction/forward
top level: BBa_K747053
start: 14
end: 211
orientation: inline

BBa_K747019
type: ComponentDefinition
persistent identity: BBa K747019
display id: BBa_K747019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 2
sequence: https://synbiohub.org/public/igem/BBa_K747019_sequence/1

AT 2
type: SequenceAnnotation
persistent identity: annotation2195984
display id: annotation2195984
version: 1
top level: BBa_K747019
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747019/annotation2195984/range2195984/1

https://synbiohub.org/public/igem/BBa_K747019/annotation2195984/range2195984/1
type: Range
persistent identity: range2195984
display id: range2195984
version: 1
direction: direction/forward
top level: BBa_K747019
start: 14
end: 211
orientation: inline

BBa_K105003
type: ComponentDefinition
persistent identity: BBa K105003
display id: BBa_K105003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR - DNA binding domain
creator: Katja Karstens
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The TetR protein is a repressor that binds to a specific operator site (BBa_R0040 & BBa_K105020). This site specific binding can be inhibited by the small molecule tetracyclin and analogs of tetracyclin.
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> The frist codon has been changed from TCC to AGT (both Ser) to prevent a methylation site. For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: We changed the format of the TetR coding part of BBa_C0040 into Phillips/Silver standard.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K105003_sequence/1

BBa_K747032
type: ComponentDefinition
persistent identity: BBa K747032
display id: BBa_K747032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 3
sequence: https://synbiohub.org/public/igem/BBa_K747032_sequence/1

AA 3
type: SequenceAnnotation
persistent identity: annotation2196207
display id: annotation2196207
version: 1
top level: BBa_K747032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747032/annotation2196207/range2196207/1

https://synbiohub.org/public/igem/BBa_K747032/annotation2196207/range2196207/1
type: Range
persistent identity: range2196207
display id: range2196207
version: 1
direction: direction/forward
top level: BBa_K747032
start: 14
end: 211
orientation: inline

BBa_K165007
type: ComponentDefinition
persistent identity: BBa K165007
display id: BBa_K165007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli-1 DNA-binding domain
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K165007_sequence/1

BBa_K747069
type: ComponentDefinition
persistent identity: BBa K747069
display id: BBa_K747069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CC5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CC 5
sequence: https://synbiohub.org/public/igem/BBa_K747069_sequence/1

CC 5
type: SequenceAnnotation
persistent identity: annotation2197032
display id: annotation2197032
version: 1
top level: BBa_K747069
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747069/annotation2197032/range2197032/1

https://synbiohub.org/public/igem/BBa_K747069/annotation2197032/range2197032/1
type: Range
persistent identity: range2197032
display id: range2197032
version: 1
direction: direction/forward
top level: BBa_K747069
start: 14
end: 211
orientation: inline

BBa_K747000
type: ComponentDefinition
persistent identity: BBa K747000
display id: BBa_K747000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA1_DiRepeat
creator: Lucas Schneider
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This Part specifies the DNA binding affinity of the 2. and 3. base pair of a Transactivator-like (TAL) protein. The TAL-Protein binds a specific fourteen base pair DNA sequence. The 2. and 3. base pair of this part is specified by adenine-adenine.
mutable notes: This part can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg.
mutable provenance: This Part was synthesized and flanked with BsmB1-restriction sites
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 1
sequence: https://synbiohub.org/public/igem/BBa_K747000_sequence/1

AA 1
type: SequenceAnnotation
persistent identity: annotation2195665
display id: annotation2195665
version: 1
top level: BBa_K747000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747000/annotation2195665/range2195665/1

https://synbiohub.org/public/igem/BBa_K747000/annotation2195665/range2195665/1
type: Range
persistent identity: range2195665
display id: range2195665
version: 1
direction: direction/forward
top level: BBa_K747000
start: 12
end: 209
orientation: inline

BBa_K165006
type: ComponentDefinition
persistent identity: BBa K165006
display id: BBa_K165006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV DNA-binding domain
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K165006_sequence/1

BBa_K747056
type: ComponentDefinition
persistent identity: BBa K747056
display id: BBa_K747056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GA4_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GA 4
sequence: https://synbiohub.org/public/igem/BBa_K747056_sequence/1

GA 4
type: SequenceAnnotation
persistent identity: annotation2197013
display id: annotation2197013
version: 1
top level: BBa_K747056
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747056/annotation2197013/range2197013/1

https://synbiohub.org/public/igem/BBa_K747056/annotation2197013/range2197013/1
type: Range
persistent identity: range2197013
display id: range2197013
version: 1
direction: direction/forward
top level: BBa_K747056
start: 14
end: 211
orientation: inline

BBa_K747020
type: ComponentDefinition
persistent identity: BBa K747020
display id: BBa_K747020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CA2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CA 2
sequence: https://synbiohub.org/public/igem/BBa_K747020_sequence/1

CA 2
type: SequenceAnnotation
persistent identity: annotation2196006
display id: annotation2196006
version: 1
top level: BBa_K747020
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747020/annotation2196006/range2196006/1

https://synbiohub.org/public/igem/BBa_K747020/annotation2196006/range2196006/1
type: Range
persistent identity: range2196006
display id: range2196006
version: 1
direction: direction/forward
top level: BBa_K747020
start: 14
end: 211
orientation: inline

BBa_K747070
type: ComponentDefinition
persistent identity: BBa K747070
display id: BBa_K747070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CG5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CG 5
sequence: https://synbiohub.org/public/igem/BBa_K747070_sequence/1

CG 5
type: SequenceAnnotation
persistent identity: annotation2197035
display id: annotation2197035
version: 1
top level: BBa_K747070
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747070/annotation2197035/range2197035/1

https://synbiohub.org/public/igem/BBa_K747070/annotation2197035/range2197035/1
type: Range
persistent identity: range2197035
display id: range2197035
version: 1
direction: direction/forward
top level: BBa_K747070
start: 14
end: 211
orientation: inline

BBa_K747087
type: ComponentDefinition
persistent identity: BBa K747087
display id: BBa_K747087
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_CT6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: Design Notes This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CT 6
sequence: https://synbiohub.org/public/igem/BBa_K747087_sequence/1

CT 6
type: SequenceAnnotation
persistent identity: annotation2197062
display id: annotation2197062
version: 1
top level: BBa_K747087
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747087/annotation2197062/range2197062/1

https://synbiohub.org/public/igem/BBa_K747087/annotation2197062/range2197062/1
type: Range
persistent identity: range2197062
display id: range2197062
version: 1
direction: direction/forward
top level: BBa_K747087
start: 14
end: 211
orientation: inline

BBa_K747080
type: ComponentDefinition
persistent identity: BBa K747080
display id: BBa_K747080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AA6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AA 6
sequence: https://synbiohub.org/public/igem/BBa_K747080_sequence/1

AA 6
type: SequenceAnnotation
persistent identity: annotation2197052
display id: annotation2197052
version: 1
top level: BBa_K747080
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747080/annotation2197052/range2197052/1

https://synbiohub.org/public/igem/BBa_K747080/annotation2197052/range2197052/1
type: Range
persistent identity: range2197052
display id: range2197052
version: 1
direction: direction/forward
top level: BBa_K747080
start: 14
end: 211
orientation: inline

BBa_K1031912
type: ComponentDefinition
persistent identity: BBa K1031912
display id: BBa_K1031912
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23105-XylS-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 105-XylS-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23105
sequence annotation: B0015
sequence annotation: XylS
sequence: https://synbiohub.org/public/igem/BBa_K1031912_sequence/1

J23105
type: SequenceAnnotation
persistent identity: annotation2360063
display id: annotation2360063
version: 1
top level: BBa_K1031912
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031912/annotation2360063/range2360063/1

https://synbiohub.org/public/igem/BBa_K1031912/annotation2360063/range2360063/1
type: Range
persistent identity: range2360063
display id: range2360063
version: 1
direction: direction/forward
top level: BBa_K1031912
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360108
display id: annotation2360108
version: 1
top level: BBa_K1031912
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031912/annotation2360108/range2360108/1

https://synbiohub.org/public/igem/BBa_K1031912/annotation2360108/range2360108/1
type: Range
persistent identity: range2360108
display id: range2360108
version: 1
direction: direction/forward
top level: BBa_K1031912
start: 1034
end: 1162
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2360107
display id: annotation2360107
version: 1
top level: BBa_K1031912
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031912/annotation2360107/range2360107/1

https://synbiohub.org/public/igem/BBa_K1031912/annotation2360107/range2360107/1
type: Range
persistent identity: range2360107
display id: range2360107
version: 1
direction: direction/forward
top level: BBa_K1031912
start: 62
end: 1028
orientation: inline

BBa_K747083
type: ComponentDefinition
persistent identity: BBa K747083
display id: BBa_K747083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 6
sequence: https://synbiohub.org/public/igem/BBa_K747083_sequence/1

AT 6
type: SequenceAnnotation
persistent identity: annotation2197056
display id: annotation2197056
version: 1
top level: BBa_K747083
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747083/annotation2197056/range2197056/1

https://synbiohub.org/public/igem/BBa_K747083/annotation2197056/range2197056/1
type: Range
persistent identity: range2197056
display id: range2197056
version: 1
direction: direction/forward
top level: BBa_K747083
start: 14
end: 211
orientation: inline

BBa_K1855004
type: ComponentDefinition
persistent identity: BBa K1855004
display id: BBa_K1855004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: INP-Zif268
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:12:50Z
discontinued: false
dominant: false
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (INP) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli.
mutable notes: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The INP domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The Zif268 fragment domain is preceeded by an NheI cut site and followed by a ClaI cut site.
mutable provenance: Lpp-OmpA is originally from Pseudomonas syringae; Zif268 is from mice.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 3x FLAG
sequence annotation: Linker
sequence annotation: BBa_K864600 T0 Terminator
sequence annotation: INP
sequence annotation: Zif268
sequence annotation: B0034 Strong RBS
sequence annotation: pL_lac
sequence: https://synbiohub.org/public/igem/BBa_K1855004_sequence/1

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466890
display id: annotation2466890
version: 1
top level: BBa_K1855004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466890/range2466890/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466890/range2466890/1
type: Range
persistent identity: range2466890
display id: range2466890
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1353
end: 1424
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466889
display id: annotation2466889
version: 1
top level: BBa_K1855004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466889/range2466889/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466889/range2466889/1
type: Range
persistent identity: range2466889
display id: range2466889
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1326
end: 1349
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466891
display id: annotation2466891
version: 1
top level: BBa_K1855004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466891/range2466891/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466891/range2466891/1
type: Range
persistent identity: range2466891
display id: range2466891
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1431
end: 1482
orientation: inline

INP
type: SequenceAnnotation
persistent identity: annotation2466886
display id: annotation2466886
version: 1
top level: BBa_K1855004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466886/range2466886/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466886/range2466886/1
type: Range
persistent identity: range2466886
display id: range2466886
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 84
end: 1007
orientation: inline

Zif268
type: SequenceAnnotation
persistent identity: annotation2466888
display id: annotation2466888
version: 1
top level: BBa_K1855004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466888/range2466888/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466888/range2466888/1
type: Range
persistent identity: range2466888
display id: range2466888
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1053
end: 1319
orientation: inline

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466885
display id: annotation2466885
version: 1
top level: BBa_K1855004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466885/range2466885/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466885/range2466885/1
type: Range
persistent identity: range2466885
display id: range2466885
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 60
end: 80
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466887
display id: annotation2466887
version: 1
top level: BBa_K1855004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466887/range2466887/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466887/range2466887/1
type: Range
persistent identity: range2466887
display id: range2466887
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1011
end: 1046
orientation: inline

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466884
display id: annotation2466884
version: 1
top level: BBa_K1855004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466884/range2466884/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466884/range2466884/1
type: Range
persistent identity: range2466884
display id: range2466884
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1
end: 56
orientation: inline

BBa_K747026
type: ComponentDefinition
persistent identity: BBa K747026
display id: BBa_K747026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GG2_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GG 2
sequence: https://synbiohub.org/public/igem/BBa_K747026_sequence/1

GG 2
type: SequenceAnnotation
persistent identity: annotation2196132
display id: annotation2196132
version: 1
top level: BBa_K747026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747026/annotation2196132/range2196132/1

https://synbiohub.org/public/igem/BBa_K747026/annotation2196132/range2196132/1
type: Range
persistent identity: range2196132
display id: range2196132
version: 1
direction: direction/forward
top level: BBa_K747026
start: 14
end: 211
orientation: inline

BBa_K747036
type: ComponentDefinition
persistent identity: BBa K747036
display id: BBa_K747036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_AT3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AT 3
sequence: https://synbiohub.org/public/igem/BBa_K747036_sequence/1

AT 3
type: SequenceAnnotation
persistent identity: annotation2196214
display id: annotation2196214
version: 1
top level: BBa_K747036
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747036/annotation2196214/range2196214/1

https://synbiohub.org/public/igem/BBa_K747036/annotation2196214/range2196214/1
type: Range
persistent identity: range2196214
display id: range2196214
version: 1
direction: direction/forward
top level: BBa_K747036
start: 14
end: 211
orientation: inline

BBa_K618212
type: ComponentDefinition
persistent identity: BBa K618212
display id: BBa_K618212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLAC ompA-zif268
creator: Ben Park, Chris Bate, Marc Ammerlaan
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _790_
m user id: 0
owner id: 8977
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a fusion between ompA and the DNA binding protein zif268 under the control of pLAC. The protein should be present on the outer surface of cells and bind the zif268 recognition sequence (GATGCTGCA). This will also allow cells to be anchored to a glass slide with the appropriate DNA attached. This can serve as a way to position cells in any desired pattern.
mutable notes: NOTE- the sequence as listed here is wrong. Because part K165006 is supplied on a Silver lab vector (Bba_J63010) with a modified biobrick prefix, the scar between ompA and zif268 is non-standard. The G at position 700 is not there. This has been verified by sequencing. Deleting the G keeps zif268 in frame with ompA. Part length is 1091bp. The sequence was generated automatically and I couldn't figure out how to edit it.
mutable provenance: parts come from iGEM distribution and from iGEM HQ
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K165006
component: BBa_J04500
component: BBa_K103006
sequence annotation: BBa_K103006
sequence annotation: BBa_J04500
sequence annotation: BBa_K165006
sequence: https://synbiohub.org/public/igem/BBa_K618212_sequence/1

BBa_K165006
type: Component
persistent identity: component2144999
display id: component2144999
version: 1
top level: BBa_K618212
access: public
definition: BBa_K165006

BBa_J04500
type: Component
persistent identity: component2144992
display id: component2144992
version: 1
top level: BBa_K618212
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K103006
type: Component
persistent identity: component2144998
display id: component2144998
version: 1
top level: BBa_K618212
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation2144998
display id: annotation2144998
version: 1
top level: BBa_K618212
location: https://synbiohub.org/public/igem/BBa_K618212/annotation2144998/range2144998/1
component: BBa_K103006

https://synbiohub.org/public/igem/BBa_K618212/annotation2144998/range2144998/1
type: Range
persistent identity: range2144998
display id: range2144998
version: 1
direction: direction/reverse
top level: BBa_K618212
start: 229
end: 692
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2144992
display id: annotation2144992
version: 1
top level: BBa_K618212
location: https://synbiohub.org/public/igem/BBa_K618212/annotation2144992/range2144992/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K618212/annotation2144992/range2144992/1
type: Range
persistent identity: range2144992
display id: range2144992
version: 1
direction: direction/reverse
top level: BBa_K618212
start: 1
end: 220
orientation: inline

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation2144999
display id: annotation2144999
version: 1
top level: BBa_K618212
location: https://synbiohub.org/public/igem/BBa_K618212/annotation2144999/range2144999/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K618212/annotation2144999/range2144999/1
type: Range
persistent identity: range2144999
display id: range2144999
version: 1
direction: direction/reverse
top level: BBa_K618212
start: 701
end: 952
orientation: inline

BBa_K1855003
type: ComponentDefinition
persistent identity: BBa K1855003
display id: BBa_K1855003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lpp-OmpA sZF14
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:12:20Z
discontinued: false
dominant: false
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (sZF14),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to TCCTCACTC on the cell surface of E.coli.
mutable notes: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The Lpp-OmpA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The sZF14 domain is preceeded by an NheI cut site and followed by a ClaI cut site.
mutable provenance: Lpp-OmpA is originally from E.coli; sZF2 is from http://www.nature.com/nmeth/journal/v10/n5/abs/nmeth.2407.html
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 3x FLAG
sequence annotation: sZF14
sequence annotation: B0034 Strong RBS
sequence annotation: BBa_K864600 T0 Terminator
sequence annotation: pL_lac
sequence annotation: Linker
sequence annotation: Lpp-OmpA
sequence: https://synbiohub.org/public/igem/BBa_K1855003_sequence/1

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466852
display id: annotation2466852
version: 1
top level: BBa_K1855003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466852/range2466852/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466852/range2466852/1
type: Range
persistent identity: range2466852
display id: range2466852
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 867
end: 938
orientation: inline

sZF14
type: SequenceAnnotation
persistent identity: annotation2466850
display id: annotation2466850
version: 1
top level: BBa_K1855003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466850/range2466850/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466850/range2466850/1
type: Range
persistent identity: range2466850
display id: range2466850
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 564
end: 833
orientation: inline

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466847
display id: annotation2466847
version: 1
top level: BBa_K1855003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466847/range2466847/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466847/range2466847/1
type: Range
persistent identity: range2466847
display id: range2466847
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 60
end: 80
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466853
display id: annotation2466853
version: 1
top level: BBa_K1855003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466853/range2466853/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466853/range2466853/1
type: Range
persistent identity: range2466853
display id: range2466853
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 945
end: 996
orientation: inline

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466846
display id: annotation2466846
version: 1
top level: BBa_K1855003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466846/range2466846/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466846/range2466846/1
type: Range
persistent identity: range2466846
display id: range2466846
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 1
end: 56
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466851
display id: annotation2466851
version: 1
top level: BBa_K1855003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466851/range2466851/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466851/range2466851/1
type: Range
persistent identity: range2466851
display id: range2466851
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 840
end: 863
orientation: inline

Lpp-OmpA
type: SequenceAnnotation
persistent identity: annotation2466848
display id: annotation2466848
version: 1
top level: BBa_K1855003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466848/range2466848/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466848/range2466848/1
type: Range
persistent identity: range2466848
display id: range2466848
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 84
end: 518
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466849
display id: annotation2466849
version: 1
top level: BBa_K1855003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466849/range2466849/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466849/range2466849/1
type: Range
persistent identity: range2466849
display id: range2466849
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 522
end: 557
orientation: inline

BBa_K747014
type: ComponentDefinition
persistent identity: BBa K747014
display id: BBa_K747014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG1_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TG 1
sequence: https://synbiohub.org/public/igem/BBa_K747014_sequence/1

TG 1
type: SequenceAnnotation
persistent identity: annotation2195929
display id: annotation2195929
version: 1
top level: BBa_K747014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747014/annotation2195929/range2195929/1

https://synbiohub.org/public/igem/BBa_K747014/annotation2195929/range2195929/1
type: Range
persistent identity: range2195929
display id: range2195929
version: 1
direction: direction/forward
top level: BBa_K747014
start: 12
end: 209
orientation: inline

BBa_K747073
type: ComponentDefinition
persistent identity: BBa K747073
display id: BBa_K747073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC5_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 5
sequence: https://synbiohub.org/public/igem/BBa_K747073_sequence/1

GC 5
type: SequenceAnnotation
persistent identity: annotation2197041
display id: annotation2197041
version: 1
top level: BBa_K747073
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747073/annotation2197041/range2197041/1

https://synbiohub.org/public/igem/BBa_K747073/annotation2197041/range2197041/1
type: Range
persistent identity: range2197041
display id: range2197041
version: 1
direction: direction/forward
top level: BBa_K747073
start: 14
end: 211
orientation: inline

BBa_K1855000
type: ComponentDefinition
persistent identity: BBa K1855000
display id: BBa_K1855000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lpp-OmpA Zif268
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:10:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli.
mutable notes: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites.
mutable provenance: Lpp-OmpA is originally from E.coli; Zif268 is from mice.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker
sequence annotation: pL_lac
sequence annotation: 3x FLAG
sequence annotation: B0034 Strong RBS
sequence annotation: BBa_K864600 T0 Terminator
sequence annotation: Lpp-OmpA
sequence annotation: Zif268
sequence: https://synbiohub.org/public/igem/BBa_K1855000_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2466652
display id: annotation2466652
version: 1
top level: BBa_K1855000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466652/range2466652/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466652/range2466652/1
type: Range
persistent identity: range2466652
display id: range2466652
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 837
end: 860
orientation: inline

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466646
display id: annotation2466646
version: 1
top level: BBa_K1855000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466646/range2466646/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466646/range2466646/1
type: Range
persistent identity: range2466646
display id: range2466646
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 1
end: 56
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466650
display id: annotation2466650
version: 1
top level: BBa_K1855000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466650/range2466650/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466650/range2466650/1
type: Range
persistent identity: range2466650
display id: range2466650
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 522
end: 557
orientation: inline

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466653
display id: annotation2466653
version: 1
top level: BBa_K1855000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466653/range2466653/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466653/range2466653/1
type: Range
persistent identity: range2466653
display id: range2466653
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 864
end: 935
orientation: inline

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466647
display id: annotation2466647
version: 1
top level: BBa_K1855000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466647/range2466647/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466647/range2466647/1
type: Range
persistent identity: range2466647
display id: range2466647
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 60
end: 80
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466654
display id: annotation2466654
version: 1
top level: BBa_K1855000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466654/range2466654/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466654/range2466654/1
type: Range
persistent identity: range2466654
display id: range2466654
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 942
end: 993
orientation: inline

Lpp-OmpA
type: SequenceAnnotation
persistent identity: annotation2466649
display id: annotation2466649
version: 1
top level: BBa_K1855000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466649/range2466649/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466649/range2466649/1
type: Range
persistent identity: range2466649
display id: range2466649
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 84
end: 518
orientation: inline

Zif268
type: SequenceAnnotation
persistent identity: annotation2466651
display id: annotation2466651
version: 1
top level: BBa_K1855000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466651/range2466651/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466651/range2466651/1
type: Range
persistent identity: range2466651
display id: range2466651
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 564
end: 830
orientation: inline

BBa_K747094
type: ComponentDefinition
persistent identity: BBa K747094
display id: BBa_K747094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 6
sequence: https://synbiohub.org/public/igem/BBa_K747094_sequence/1

TC 6
type: SequenceAnnotation
persistent identity: annotation2197072
display id: annotation2197072
version: 1
top level: BBa_K747094
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747094/annotation2197072/range2197072/1

https://synbiohub.org/public/igem/BBa_K747094/annotation2197072/range2197072/1
type: Range
persistent identity: range2197072
display id: range2197072
version: 1
direction: direction/forward
top level: BBa_K747094
start: 14
end: 211
orientation: inline

BBa_K801036
type: ComponentDefinition
persistent identity: BBa K801036
display id: BBa_K801036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Linker-LexA
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of composite part of Bba_K365005 and BBa_K801033
mutable notes: -
mutable provenance: Composite part of composite part of Bba_K365005 and BBa_K801033
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K365005
sequence annotation: RFC25 fusion scar
sequence annotation: BBa_K801033
sequence: https://synbiohub.org/public/igem/BBa_K801036_sequence/1

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2198891
display id: annotation2198891
version: 1
top level: BBa_K801036
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801036/annotation2198891/range2198891/1

https://synbiohub.org/public/igem/BBa_K801036/annotation2198891/range2198891/1
type: Range
persistent identity: range2198891
display id: range2198891
version: 1
direction: direction/forward
top level: BBa_K801036
start: 1
end: 60
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198895
display id: annotation2198895
version: 1
top level: BBa_K801036
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801036/annotation2198895/range2198895/1

https://synbiohub.org/public/igem/BBa_K801036/annotation2198895/range2198895/1
type: Range
persistent identity: range2198895
display id: range2198895
version: 1
direction: direction/forward
top level: BBa_K801036
start: 61
end: 66
orientation: inline

BBa_K801033
type: SequenceAnnotation
persistent identity: annotation2198892
display id: annotation2198892
version: 1
top level: BBa_K801036
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801036/annotation2198892/range2198892/1

https://synbiohub.org/public/igem/BBa_K801036/annotation2198892/range2198892/1
type: Range
persistent identity: range2198892
display id: range2198892
version: 1
direction: direction/forward
top level: BBa_K801036
start: 67
end: 669
orientation: inline

BBa_K747046
type: ComponentDefinition
persistent identity: BBa K747046
display id: BBa_K747046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG3_Direpeat
creator: Lucas Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:10Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: following
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TG 3
sequence: https://synbiohub.org/public/igem/BBa_K747046_sequence/1

TG 3
type: SequenceAnnotation
persistent identity: annotation2196268
display id: annotation2196268
version: 1
top level: BBa_K747046
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747046/annotation2196268/range2196268/1

https://synbiohub.org/public/igem/BBa_K747046/annotation2196268/range2196268/1
type: Range
persistent identity: range2196268
display id: range2196268
version: 1
direction: direction/forward
top level: BBa_K747046
start: 14
end: 211
orientation: inline

BBa_K618111
type: ComponentDefinition
persistent identity: BBa K618111
display id: BBa_K618111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD ompA-Gli1
creator: Chris Bate, Ben Park, Marc Ammerlaan
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _790_
m user id: 0
owner id: 8977
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a fusion between ompA and the DNA binding protein Gli-1. It should be present on the outer surface of cells and bind the Gli-! recognition sequence there. This will also allows cells to be anchored to glass slide with the DNA attached.
mutable notes: Silver prefix on Gli-1 allowed us to fuse it to ompA in frame.
mutable provenance: parts come from iGEM distribution
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K259007
component: BBa_K103006
component: BBa_K165007
sequence annotation: BBa_K259007
sequence annotation: BBa_K165007
sequence annotation: BBa_K103006
sequence: https://synbiohub.org/public/igem/BBa_K618111_sequence/1

BBa_K259007
type: Component
persistent identity: component2144556
display id: component2144556
version: 1
top level: BBa_K618111
access: public
definition: https://synbiohub.org/public/igem/BBa_K259007/1

BBa_K103006
type: Component
persistent identity: component2144562
display id: component2144562
version: 1
top level: BBa_K618111
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_K165007
type: Component
persistent identity: component2144563
display id: component2144563
version: 1
top level: BBa_K618111
access: public
definition: BBa_K165007

BBa_K259007
type: SequenceAnnotation
persistent identity: annotation2144556
display id: annotation2144556
version: 1
top level: BBa_K618111
location: https://synbiohub.org/public/igem/BBa_K618111/annotation2144556/range2144556/1
component: BBa_K259007

https://synbiohub.org/public/igem/BBa_K618111/annotation2144556/range2144556/1
type: Range
persistent identity: range2144556
display id: range2144556
version: 1
direction: direction/reverse
top level: BBa_K618111
start: 1
end: 360
orientation: inline

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation2144563
display id: annotation2144563
version: 1
top level: BBa_K618111
location: https://synbiohub.org/public/igem/BBa_K618111/annotation2144563/range2144563/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K618111/annotation2144563/range2144563/1
type: Range
persistent identity: range2144563
display id: range2144563
version: 1
direction: direction/reverse
top level: BBa_K618111
start: 841
end: 1365
orientation: inline

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation2144562
display id: annotation2144562
version: 1
top level: BBa_K618111
location: https://synbiohub.org/public/igem/BBa_K618111/annotation2144562/range2144562/1
component: BBa_K103006

https://synbiohub.org/public/igem/BBa_K618111/annotation2144562/range2144562/1
type: Range
persistent identity: range2144562
display id: range2144562
version: 1
direction: direction/reverse
top level: BBa_K618111
start: 369
end: 832
orientation: inline

BBa_K747089
type: ComponentDefinition
persistent identity: BBa K747089
display id: BBa_K747089
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_GC6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GC 6
sequence: https://synbiohub.org/public/igem/BBa_K747089_sequence/1

GC 6
type: SequenceAnnotation
persistent identity: annotation2197065
display id: annotation2197065
version: 1
top level: BBa_K747089
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747089/annotation2197065/range2197065/1

https://synbiohub.org/public/igem/BBa_K747089/annotation2197065/range2197065/1
type: Range
persistent identity: range2197065
display id: range2197065
version: 1
direction: direction/forward
top level: BBa_K747089
start: 14
end: 211
orientation: inline