Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
LacI promoter with RBS, followed by OmpA outer membrane protein and linker for Escherichia coli cell surface display.
Composite part BBa_K392001 (LacI promoter + RBS) and registry part BBa_K103006 (OmpA w/ linker).
This S-tag can be added between two protein parts. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of S-tag> basic part K112705 Wobble PCR dv013/dv014 (47 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+47, L) Product is pBca1256-K112705 ----------------------------------------------- dv013 Forward Biobricking of S-Tag> cgataGAATTCatgAGATCTatgaaagaaaccgctgctgctaaattcg dv014 Reverse Biobricking of S-Tag> cgttaGGATCCgctgtccatgtgctggcgttcgaatttagcagcagcgg
His-tag with amino acid Methionine.His-tag is used for detection of His-tagged proteins by anti-His antibodies.
Part was constructed by primer annealing.
V5 epitope tag head domain (GKPIPNPLLGLDST)
This is a V5 epitope tag head domain designed to be at the N-terminus of a protein. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.
This head domain begins with Met-Lys to promote efficient translation of the protein in E. coli. Rare codons from various model organisms were avoided where possible.
EnvZ Signal Peptide and first Transmembrane Domain
For EnvZ fusion protein design. Long description will be added soon.
Source: E. coli EnvZ protein (ncbi: https://www.ncbi.nlm.nih.gov/gene/947272)
This is a c-Myc tag head domain designed to be at the N-terminus of a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
This tail domain encodes the c-Myc sequence EQKLISEEDL. The codons at each amino acid position were selected to avoid rare codons in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts.
c-myc tag is used for detection of proteins by anti-c-myc antibodies.
Gene was designed to be constructed by primer annealing. Includes start codone.
This is a 6x His tag head domain designed to encode the N-terminus of a protein. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
Both Histidine codons were used to minimize repeats. An ATG start codon was used.
This is a Strep tag head domain designed to be at the N-terminus of a protein. Strep tags are typically used for detection, purification and immobilization of proteins.
This domain encodes the Strep tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
HA-tag is used for detection of HA-tagged proteins by anti-HA antibodies.
Gene was designed to be constructed by primer annealing. Includes start codon.
This his tag can be fused to the N terminus of a protein. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of His-Tag> basic part K112703 Mix dv009/dv010 (41bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+41, L) Product is pBca1256-K112703 ----------------------------------------------- dv009 Forward Biobricking of His-Tag> cgataGAATTCatgAGATCTatgCATCATCATCATCATCATGGATCCtaacg dv010 Reverse Biobricking of His-Tag> cgttaGGATCCATGATGATGATGATGATGcatAGATCTcatGAATTCtatgg
hemagglutinin (HA) tag head domain (YPYDVPDYA)
This is a HA tag head domain designed to be at the N-terminus of a protein. HA tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
This domain encodes the HA sequence YPYDVPDYA. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
This is a S-tag head domain designed to be at the N-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
This head domain begins with Met-Lys to promote efficient translation of the protein in ''E. coli''.
EGFR/ErbB1 signal peptide; mediates protein transport to a translocational pore
The N-terminal signal-peptide ensures protein transport to the cytoplasm-membrane.
Part derived from the sequence of EGFR/ErbB1