//rbs/prokaryote/constitutive/community
type: Collection
persistent identity: rbs prokaryote constitutive community
display id: rbs_prokaryote_constitutive_community
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_B0033
member: BBa_K1390019
member: nickname
member: BBa_B0031
member: BBa_B0030
member: BBa_K2122000
member: BBa_B0035
member: BBa_B0034
member: BBa_B0064
member: BBa_B0032
member: BBa_J23080
member: BBa_B0029
member: BBa_K2122100

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.4 (weaker) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
direction: direction/forward
top level: BBa_B0033
start: 7
end: 10
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.3 (medium) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
direction: direction/forward
top level: BBa_B0032
start: 7
end: 10
orientation: inline

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

BBa_B0029
type: ComponentDefinition
persistent identity: BBa B0029
display id: BBa_B0029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1 bp deletion from B0031
creator: Jason Kelly
created: 2008-02-07T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0029_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS (Elowitz 1999) -- defines RBS efficiency
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS based on Elowitz repressilator.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
direction: direction/forward
top level: BBa_B0034
start: 5
end: 8
orientation: inline

BBa_K2122000
type: ComponentDefinition
persistent identity: BBa K2122000
display id: BBa_K2122000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter
creator: Sannia Farrukh
created: 2016-09-28T11:00:00Z
modified: 2016-10-18T01:26:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2591_
m user id: 32074
owner id: 32074
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
mutable notes: An inducible promoter (BBa_10500), RBS (BBa_B0034) Scar was added to the sequence. Along with a terminator.
mutable provenance: Coding Sequence from GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K2122150
component: BBa_B0034
component: BBa_I0500
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K2122150
sequence: https://synbiohub.org/public/igem/BBa_K2122000_sequence/1

BBa_K2122150
type: Component
persistent identity: component2517225
display id: component2517225
version: 1
top level: BBa_K2122000
access: public
definition: https://synbiohub.org/public/igem/BBa_K2122150/1

BBa_B0034
type: Component
persistent identity: component2517224
display id: component2517224
version: 1
top level: BBa_K2122000
access: public
definition: BBa_B0034

BBa_I0500
type: Component
persistent identity: component2517222
display id: component2517222
version: 1
top level: BBa_K2122000
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0014
type: Component
persistent identity: component2517232
display id: component2517232
version: 1
top level: BBa_K2122000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2517232
display id: annotation2517232
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517232/range2517232/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517232/range2517232/1
type: Range
persistent identity: range2517232
display id: range2517232
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 2307
end: 2401
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2517224
display id: annotation2517224
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517224/range2517224/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517224/range2517224/1
type: Range
persistent identity: range2517224
display id: range2517224
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 1219
end: 1230
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2517222
display id: annotation2517222
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517222/range2517222/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517222/range2517222/1
type: Range
persistent identity: range2517222
display id: range2517222
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 1
end: 1210
orientation: inline

BBa_K2122150
type: SequenceAnnotation
persistent identity: annotation2517225
display id: annotation2517225
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517225/range2517225/1
component: BBa_K2122150

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517225/range2517225/1
type: Range
persistent identity: range2517225
display id: range2517225
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 1237
end: 2298
orientation: inline

BBa_B0035
type: ComponentDefinition
persistent identity: BBa B0035
display id: BBa_B0035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS (B0030 derivative)
creator: Jason Kelly
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0035_sequence/1

BBa_J23080
type: ComponentDefinition
persistent identity: BBa J23080
display id: BBa_J23080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: b0034 derived RBS to match spacing of J01122
creator: bryan hernandez
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _52_
m user id: 0
owner id: 936
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122.
mutable notes: none
mutable provenance: j01122
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J23080_sequence/1

BBa_K2122100
type: ComponentDefinition
persistent identity: BBa K2122100
display id: BBa_K2122100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Device expressing the Gb3 Synthase enzyme under control of a constitutive promoter
creator: Sannia Farrukh
created: 2016-09-28T11:00:00Z
modified: 2016-10-18T01:30:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2591_
m user id: 32074
owner id: 32074
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
mutable notes: A constitutive promoter was added to the sequence
mutable provenance: Coding Sequence from GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_B0014
component: BBa_K2122150
component: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_B0014
sequence annotation: BBa_J23100
sequence annotation: BBa_K2122150
sequence: https://synbiohub.org/public/igem/BBa_K2122100_sequence/1

BBa_B0034
type: Component
persistent identity: component2518015
display id: component2518015
version: 1
top level: BBa_K2122100
access: public
definition: BBa_B0034

BBa_B0014
type: Component
persistent identity: component2518023
display id: component2518023
version: 1
top level: BBa_K2122100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_K2122150
type: Component
persistent identity: component2518016
display id: component2518016
version: 1
top level: BBa_K2122100
access: public
definition: https://synbiohub.org/public/igem/BBa_K2122150/1

BBa_J23100
type: Component
persistent identity: component2518013
display id: component2518013
version: 1
top level: BBa_K2122100
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518015
display id: annotation2518015
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518015/range2518015/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518015/range2518015/1
type: Range
persistent identity: range2518015
display id: range2518015
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 44
end: 55
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2518023
display id: annotation2518023
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518023/range2518023/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518023/range2518023/1
type: Range
persistent identity: range2518023
display id: range2518023
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 1132
end: 1226
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2518013
display id: annotation2518013
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518013/range2518013/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518013/range2518013/1
type: Range
persistent identity: range2518013
display id: range2518013
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 1
end: 35
orientation: inline

BBa_K2122150
type: SequenceAnnotation
persistent identity: annotation2518016
display id: annotation2518016
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518016/range2518016/1
component: BBa_K2122150

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518016/range2518016/1
type: Range
persistent identity: range2518016
display id: range2518016
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 62
end: 1123
orientation: inline

BBa_K1390019
type: ComponentDefinition
persistent identity: BBa K1390019
display id: BBa_K1390019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Complete sMMO of Methylococcus capsulatus
creator: Torsten Meyer
created: 2014-09-03T11:00:00Z
modified: 2015-05-08T01:10:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The part consists of the six subunits of methane monooxygenase (MMO). Gene order: mmoX, mmoY, mmoB, mmoZ, mmoD, mmoC The expression is under control of lac+pl. MMO converts methane to methanol.
mutable notes: Part is designed considering the RFC10 standard.
mutable provenance: Methylococcus capsulatus strain Bath
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1390001
component: BBa_K1390003
component: BBa_B0032
component: BBa_K1390005
component: BBa_R0011
component: BBa_K1390002
component: BBa_K1390006
component: BBa_B0015
component: BBa_K1390004
sequence annotation: BBa_B0032
sequence annotation: BBa_K1390005
sequence annotation: BBa_K1390006
sequence annotation: BBa_K1390004
sequence annotation: BBa_K1390003
sequence annotation: BBa_B0015
sequence annotation: BBa_K1390001
sequence annotation: BBa_K1390002
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K1390019_sequence/1

BBa_K1390001
type: Component
persistent identity: component2412365
display id: component2412365
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390001/1

BBa_K1390003
type: Component
persistent identity: component2412373
display id: component2412373
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390003/1

BBa_B0032
type: Component
persistent identity: component2412367
display id: component2412367
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412359
display id: component2412359
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412371
display id: component2412371
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_K1390005
type: Component
persistent identity: component2412361
display id: component2412361
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390005/1

BBa_B0032
type: Component
persistent identity: component2412355
display id: component2412355
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412363
display id: component2412363
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412375
display id: component2412375
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_R0011
type: Component
persistent identity: component2412349
display id: component2412349
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_K1390002
type: Component
persistent identity: component2412377
display id: component2412377
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390002/1

BBa_K1390006
type: Component
persistent identity: component2412369
display id: component2412369
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390006/1

BBa_B0015
type: Component
persistent identity: component2412384
display id: component2412384
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1390004
type: Component
persistent identity: component2412357
display id: component2412357
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390004/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412371
display id: annotation2412371
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412371/range2412371/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412371/range2412371/1
type: Range
persistent identity: range2412371
display id: range2412371
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 3862
end: 3874
orientation: inline

BBa_K1390005
type: SequenceAnnotation
persistent identity: annotation2412361
display id: annotation2412361
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412361/range2412361/1
component: BBa_K1390005

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412361/range2412361/1
type: Range
persistent identity: range2412361
display id: range2412361
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 1694
end: 2863
orientation: inline

BBa_K1390006
type: SequenceAnnotation
persistent identity: annotation2412369
display id: annotation2412369
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412369/range2412369/1
component: BBa_K1390006

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412369/range2412369/1
type: Range
persistent identity: range2412369
display id: range2412369
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 3344
end: 3853
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412367
display id: annotation2412367
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412367/range2412367/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412367/range2412367/1
type: Range
persistent identity: range2412367
display id: range2412367
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 3325
end: 3337
orientation: inline

BBa_K1390004
type: SequenceAnnotation
persistent identity: annotation2412357
display id: annotation2412357
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412357/range2412357/1
component: BBa_K1390004

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412357/range2412357/1
type: Range
persistent identity: range2412357
display id: range2412357
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 83
end: 1666
orientation: inline

BBa_K1390003
type: SequenceAnnotation
persistent identity: annotation2412373
display id: annotation2412373
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412373/range2412373/1
component: BBa_K1390003

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412373/range2412373/1
type: Range
persistent identity: range2412373
display id: range2412373
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 3881
end: 4192
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2412384
display id: annotation2412384
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412384/range2412384/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412384/range2412384/1
type: Range
persistent identity: range2412384
display id: range2412384
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 5275
end: 5403
orientation: inline

BBa_K1390001
type: SequenceAnnotation
persistent identity: annotation2412365
display id: annotation2412365
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412365/range2412365/1
component: BBa_K1390001

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412365/range2412365/1
type: Range
persistent identity: range2412365
display id: range2412365
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 2891
end: 3316
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412375
display id: annotation2412375
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412375/range2412375/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412375/range2412375/1
type: Range
persistent identity: range2412375
display id: range2412375
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 4201
end: 4213
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412355
display id: annotation2412355
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412355/range2412355/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412355/range2412355/1
type: Range
persistent identity: range2412355
display id: range2412355
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 64
end: 76
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412363
display id: annotation2412363
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412363/range2412363/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412363/range2412363/1
type: Range
persistent identity: range2412363
display id: range2412363
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 2872
end: 2884
orientation: inline

BBa_K1390002
type: SequenceAnnotation
persistent identity: annotation2412377
display id: annotation2412377
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412377/range2412377/1
component: BBa_K1390002

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412377/range2412377/1
type: Range
persistent identity: range2412377
display id: range2412377
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 4220
end: 5266
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2412349
display id: annotation2412349
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412349/range2412349/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412349/range2412349/1
type: Range
persistent identity: range2412349
display id: range2412349
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412359
display id: annotation2412359
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412359/range2412359/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412359/range2412359/1
type: Range
persistent identity: range2412359
display id: range2412359
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 1675
end: 1687
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.2 (weak) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
direction: direction/forward
top level: BBa_B0031
start: 7
end: 10
orientation: inline

BBa_B0064
type: ComponentDefinition
persistent identity: BBa B0064
display id: BBa_B0064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tuned RBS for Q04401
creator: Jason Kelly
created: 2008-02-23T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a single bp change from B0034. It's strength is approximately 35% that of B0034.
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0064_sequence/1

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.1 (strong) -- modified from R. Weiss
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
direction: direction/forward
top level: BBa_B0030
start: 8
end: 12
orientation: inline

nickname
type: ComponentDefinition
persistent identity: BBa K1479004
display id: BBa_K1479004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Just for upload test
creator: ChenYB
created: 2014-09-30T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is just for upload test. It is meaningless
mutable notes: NULL
mutable provenance: NULL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: misc
sequence annotation: PolyA
sequence annotation: polya
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: BBa_B1008
sequence annotation: stop
sequence annotation: BBa_B0011
sequence annotation: T7 TE
sequence annotation: BBa_B0014
sequence annotation: 22
sequence annotation: BBa_B0015
sequence annotation: BBa_B1007
sequence annotation: BBa_K1479001
sequence annotation: modified thr terminator
sequence annotation: BBa_K1479002
sequence annotation: BBa_B0010
sequence annotation: modified thr
sequence annotation: B1007
sequence annotation: B1008
sequence: https://synbiohub.org/public/igem/BBa_K1479004_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2397990
display id: annotation2397990
version: 1
top level: nickname
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397990/range2397990/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397990/range2397990/1
type: Range
persistent identity: range2397990
display id: range2397990
version: 1
direction: direction/reverse
top level: nickname
start: 1
end: 20
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397970
display id: annotation2397970
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397970/range2397970/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397970/range2397970/1
type: Range
persistent identity: range2397970
display id: range2397970
version: 1
direction: direction/forward
top level: nickname
start: 135
end: 139
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397988
display id: annotation2397988
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397988/range2397988/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397988/range2397988/1
type: Range
persistent identity: range2397988
display id: range2397988
version: 1
direction: direction/forward
top level: nickname
start: 418
end: 431
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397964
display id: annotation2397964
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397964/range2397964/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397964/range2397964/1
type: Range
persistent identity: range2397964
display id: range2397964
version: 1
direction: direction/forward
top level: nickname
start: 62
end: 84
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397959
display id: annotation2397959
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397959/range2397959/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397959/range2397959/1
type: Range
persistent identity: range2397959
display id: range2397959
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 41
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397976
display id: annotation2397976
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397976/range2397976/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397976/range2397976/1
type: Range
persistent identity: range2397976
display id: range2397976
version: 1
direction: direction/forward
top level: nickname
start: 200
end: 240
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397968
display id: annotation2397968
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397968/range2397968/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397968/range2397968/1
type: Range
persistent identity: range2397968
display id: range2397968
version: 1
direction: direction/reverse
top level: nickname
start: 108
end: 112
orientation: inline

BBa_B1008
type: SequenceAnnotation
persistent identity: annotation2397967
display id: annotation2397967
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397967/range2397967/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397967/range2397967/1
type: Range
persistent identity: range2397967
display id: range2397967
version: 1
direction: direction/reverse
top level: nickname
start: 104
end: 143
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397989
display id: annotation2397989
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397989/range2397989/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397989/range2397989/1
type: Range
persistent identity: range2397989
display id: range2397989
version: 1
direction: direction/forward
top level: nickname
start: 424
end: 424
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397982
display id: annotation2397982
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397982/range2397982/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397982/range2397982/1
type: Range
persistent identity: range2397982
display id: range2397982
version: 1
direction: direction/forward
top level: nickname
start: 261
end: 283
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2397963
display id: annotation2397963
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397963/range2397963/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397963/range2397963/1
type: Range
persistent identity: range2397963
display id: range2397963
version: 1
direction: direction/forward
top level: nickname
start: 50
end: 95
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397978
display id: annotation2397978
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397978/range2397978/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397978/range2397978/1
type: Range
persistent identity: range2397978
display id: range2397978
version: 1
direction: direction/forward
top level: nickname
start: 207
end: 226
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397980
display id: annotation2397980
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397980/range2397980/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397980/range2397980/1
type: Range
persistent identity: range2397980
display id: range2397980
version: 1
direction: direction/forward
top level: nickname
start: 233
end: 233
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2397977
display id: annotation2397977
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397977/range2397977/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397977/range2397977/1
type: Range
persistent identity: range2397977
display id: range2397977
version: 1
direction: direction/reverse
top level: nickname
start: 200
end: 294
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397961
display id: annotation2397961
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397961/range2397961/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397961/range2397961/1
type: Range
persistent identity: range2397961
display id: range2397961
version: 1
direction: direction/forward
top level: nickname
start: 28
end: 41
orientation: inline

22
type: SequenceAnnotation
persistent identity: annotation2397957
display id: annotation2397957
version: 1
top level: nickname
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397957/range2397957/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397957/range2397957/1
type: Range
persistent identity: range2397957
display id: range2397957
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 10
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2397984
display id: annotation2397984
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397984/range2397984/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397984/range2397984/1
type: Range
persistent identity: range2397984
display id: range2397984
version: 1
direction: direction/reverse
top level: nickname
start: 303
end: 431
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397985
display id: annotation2397985
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397985/range2397985/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397985/range2397985/1
type: Range
persistent identity: range2397985
display id: range2397985
version: 1
direction: direction/forward
top level: nickname
start: 314
end: 357
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2397981
display id: annotation2397981
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397981/range2397981/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397981/range2397981/1
type: Range
persistent identity: range2397981
display id: range2397981
version: 1
direction: direction/forward
top level: nickname
start: 249
end: 294
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397962
display id: annotation2397962
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397962/range2397962/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397962/range2397962/1
type: Range
persistent identity: range2397962
display id: range2397962
version: 1
direction: direction/forward
top level: nickname
start: 34
end: 34
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2397956
display id: annotation2397956
version: 1
top level: nickname
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397956/range2397956/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397956/range2397956/1
type: Range
persistent identity: range2397956
display id: range2397956
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 20
orientation: inline

BBa_B1007
type: SequenceAnnotation
persistent identity: annotation2397971
display id: annotation2397971
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397971/range2397971/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397971/range2397971/1
type: Range
persistent identity: range2397971
display id: range2397971
version: 1
direction: direction/reverse
top level: nickname
start: 152
end: 191
orientation: inline

BBa_K1479001
type: SequenceAnnotation
persistent identity: annotation2397965
display id: annotation2397965
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397965/range2397965/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397965/range2397965/1
type: Range
persistent identity: range2397965
display id: range2397965
version: 1
direction: direction/reverse
top level: nickname
start: 104
end: 431
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397979
display id: annotation2397979
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397979/range2397979/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397979/range2397979/1
type: Range
persistent identity: range2397979
display id: range2397979
version: 1
direction: direction/forward
top level: nickname
start: 227
end: 240
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation2397969
display id: annotation2397969
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397969/range2397969/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397969/range2397969/1
type: Range
persistent identity: range2397969
display id: range2397969
version: 1
direction: direction/forward
top level: nickname
start: 113
end: 134
orientation: inline

BBa_K1479002
type: SequenceAnnotation
persistent identity: annotation2397955
display id: annotation2397955
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397955/range2397955/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397955/range2397955/1
type: Range
persistent identity: range2397955
display id: range2397955
version: 1
direction: direction/reverse
top level: nickname
start: 1
end: 95
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2397983
display id: annotation2397983
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397983/range2397983/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397983/range2397983/1
type: Range
persistent identity: range2397983
display id: range2397983
version: 1
direction: direction/forward
top level: nickname
start: 303
end: 382
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397987
display id: annotation2397987
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397987/range2397987/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397987/range2397987/1
type: Range
persistent identity: range2397987
display id: range2397987
version: 1
direction: direction/forward
top level: nickname
start: 398
end: 417
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397986
display id: annotation2397986
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397986/range2397986/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397986/range2397986/1
type: Range
persistent identity: range2397986
display id: range2397986
version: 1
direction: direction/forward
top level: nickname
start: 391
end: 431
orientation: inline

modified thr
type: SequenceAnnotation
persistent identity: annotation2397974
display id: annotation2397974
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397974/range2397974/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397974/range2397974/1
type: Range
persistent identity: range2397974
display id: range2397974
version: 1
direction: direction/forward
top level: nickname
start: 161
end: 182
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397975
display id: annotation2397975
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397975/range2397975/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397975/range2397975/1
type: Range
persistent identity: range2397975
display id: range2397975
version: 1
direction: direction/forward
top level: nickname
start: 183
end: 188
orientation: inline

B1007
type: SequenceAnnotation
persistent identity: annotation2397972
display id: annotation2397972
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397972/range2397972/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397972/range2397972/1
type: Range
persistent identity: range2397972
display id: range2397972
version: 1
direction: direction/forward
top level: nickname
start: 152
end: 191
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397973
display id: annotation2397973
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397973/range2397973/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397973/range2397973/1
type: Range
persistent identity: range2397973
display id: range2397973
version: 1
direction: direction/reverse
top level: nickname
start: 155
end: 160
orientation: inline

B1008
type: SequenceAnnotation
persistent identity: annotation2397966
display id: annotation2397966
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397966/range2397966/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397966/range2397966/1
type: Range
persistent identity: range2397966
display id: range2397966
version: 1
direction: direction/forward
top level: nickname
start: 104
end: 143
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397960
display id: annotation2397960
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397960/range2397960/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397960/range2397960/1
type: Range
persistent identity: range2397960
display id: range2397960
version: 1
direction: direction/forward
top level: nickname
start: 8
end: 27
orientation: inline