rbs_prokaryote_constitutive_community
1
//rbs/prokaryote/constitutive/community
BBa_B0029
1
BBa_B0029
1 bp deletion from B0031
Jason Kelly
2008-02-07T12:00:00Z
2015-08-31T04:07:20Z
false
false
_11_
0
2
84
Not in stock
false
.
.
.
false
BBa_B0035
1
BBa_B0035
RBS (B0030 derivative)
Jason Kelly
2004-01-27T12:00:00Z
2015-08-31T04:07:20Z
false
false
_1_
0
24
7
Not in stock
false
-- No description --
false
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_44_46_
0
24
7
Released HQ 2013
In stock
false
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_J23080
1
BBa_J23080
b0034 derived RBS to match spacing of J01122
bryan hernandez
2006-09-20T11:00:00Z
2015-08-31T04:08:40Z
false
false
_52_
0
936
52
In stock
false
RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122.
none
j01122
false
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_41_44_48_46_1_
0
24
7
Released HQ 2013
In stock
false
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
annotation23316
1
conserved
range23316
1
7
10
BBa_B0033
1
BBa_B0033
RBS.4 (weaker) -- derivative of BBa_0030
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_41_44_48_46_1_
0
24
7
Released HQ 2013
In stock
false
Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
annotation7028
1
BBa_B0033
range7028
1
1
11
annotation1713
1
RBS-4\Weaker
range1713
1
1
11
annotation1714
1
RBS
range1714
1
7
10
BBa_K2122000
1
BBa_K2122000
Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter
Sannia Farrukh
2016-09-28T11:00:00Z
2016-10-18T01:26:30Z
false
false
_2591_
32074
32074
9
true
This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
An inducible promoter (BBa_10500), RBS (BBa_B0034) Scar was added to the sequence. Along with a terminator.
Coding Sequence from GenBank
false
component2517222
1
BBa_I0500
component2517232
1
BBa_B0014
component2517225
1
BBa_K2122150
component2517224
1
BBa_B0034
annotation2517224
1
BBa_B0034
range2517224
1
1219
1230
annotation2517222
1
BBa_I0500
range2517222
1
1
1210
annotation2517232
1
BBa_B0014
range2517232
1
2307
2401
annotation2517225
1
BBa_K2122150
range2517225
1
1237
2298
BBa_K2122100
1
BBa_K2122100
Device expressing the Gb3 Synthase enzyme under control of a constitutive promoter
Sannia Farrukh
2016-09-28T11:00:00Z
2016-10-18T01:30:37Z
false
false
_2591_
32074
32074
9
false
This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
A constitutive promoter was added to the sequence
Coding Sequence from GenBank
false
component2518013
1
BBa_J23100
component2518015
1
BBa_B0034
component2518023
1
BBa_B0014
component2518016
1
BBa_K2122150
annotation2518015
1
BBa_B0034
range2518015
1
44
55
annotation2518016
1
BBa_K2122150
range2518016
1
62
1123
annotation2518023
1
BBa_B0014
range2518023
1
1132
1226
annotation2518013
1
BBa_J23100
range2518013
1
1
35
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_1_
0
24
7
Released HQ 2013
In stock
false
RBS based on Elowitz repressilator.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
annotation23325
1
conserved
range23325
1
5
8
BBa_B0064
1
BBa_B0064
Tuned RBS for Q04401
Jason Kelly
2008-02-23T12:00:00Z
2015-08-31T04:07:21Z
false
false
_11_
0
2
84
Not in stock
false
This is a single bp change from B0034. It's strength is approximately 35% that of B0034.
.
.
false
BBa_K1390019
1
BBa_K1390019
Complete sMMO of Methylococcus capsulatus
Torsten Meyer
2014-09-03T11:00:00Z
2015-05-08T01:10:14Z
false
false
_1767_
0
17096
9
It's complicated
true
The part consists of the six subunits of methane monooxygenase (MMO).
Gene order: mmoX, mmoY, mmoB, mmoZ, mmoD, mmoC
The expression is under control of lac+pl.
MMO converts methane to methanol.
Part is designed considering the RFC10 standard.
Methylococcus capsulatus strain Bath
false
component2412355
1
BBa_B0032
component2412367
1
BBa_B0032
component2412357
1
BBa_K1390004
component2412375
1
BBa_B0032
component2412349
1
BBa_R0011
component2412377
1
BBa_K1390002
component2412365
1
BBa_K1390001
component2412373
1
BBa_K1390003
component2412359
1
BBa_B0032
component2412384
1
BBa_B0015
component2412361
1
BBa_K1390005
component2412363
1
BBa_B0032
component2412369
1
BBa_K1390006
component2412371
1
BBa_B0032
annotation2412371
1
BBa_B0032
range2412371
1
3862
3874
annotation2412361
1
BBa_K1390005
range2412361
1
1694
2863
annotation2412375
1
BBa_B0032
range2412375
1
4201
4213
annotation2412367
1
BBa_B0032
range2412367
1
3325
3337
annotation2412357
1
BBa_K1390004
range2412357
1
83
1666
annotation2412373
1
BBa_K1390003
range2412373
1
3881
4192
annotation2412365
1
BBa_K1390001
range2412365
1
2891
3316
annotation2412384
1
BBa_B0015
range2412384
1
5275
5403
annotation2412377
1
BBa_K1390002
range2412377
1
4220
5266
annotation2412369
1
BBa_K1390006
range2412369
1
3344
3853
annotation2412355
1
BBa_B0032
range2412355
1
64
76
annotation2412363
1
BBa_B0032
range2412363
1
2872
2884
annotation2412349
1
BBa_R0011
range2412349
1
1
54
annotation2412359
1
BBa_B0032
range2412359
1
1675
1687
BBa_K1479004
1
nickname
Just for upload test
ChenYB
2014-09-30T11:00:00Z
2015-05-08T01:10:40Z
false
false
_1859_
0
21515
9
Not in stock
false
This part is just for upload test. It is meaningless
NULL
NULL
false
annotation2397970
1
PolyA
range2397970
1
135
139
annotation2397988
1
polya
range2397988
1
418
431
annotation2397980
1
stop
range2397980
1
233
233
annotation2397990
1
misc
range2397990
1
1
20
annotation2397959
1
BBa_B0012
range2397959
1
1
41
annotation2397976
1
BBa_B0012
range2397976
1
200
240
annotation2397966
1
B1008
range2397966
1
104
143
annotation2397967
1
BBa_B1008
range2397967
1
104
143
annotation2397982
1
stem_loop
range2397982
1
261
283
annotation2397978
1
T7 TE
range2397978
1
207
226
annotation2397964
1
stem_loop
range2397964
1
62
84
annotation2397977
1
BBa_B0014
range2397977
1
200
294
annotation2397961
1
polya
range2397961
1
28
41
annotation2397984
1
BBa_B0015
range2397984
1
303
431
annotation2397989
1
stop
range2397989
1
424
424
annotation2397974
1
modified thr
range2397974
1
161
182
annotation2397981
1
BBa_B0011
range2397981
1
249
294
annotation2397985
1
stem_loop
range2397985
1
314
357
annotation2397975
1
PolyA
range2397975
1
183
188
annotation2397963
1
BBa_B0011
range2397963
1
50
95
annotation2397962
1
stop
range2397962
1
34
34
annotation2397956
1
misc
range2397956
1
1
20
annotation2397971
1
BBa_B1007
range2397971
1
152
191
annotation2397965
1
BBa_K1479001
range2397965
1
104
431
annotation2397979
1
polya
range2397979
1
227
240
annotation2397969
1
modified thr terminator
range2397969
1
113
134
annotation2397955
1
BBa_K1479002
range2397955
1
1
95
annotation2397987
1
T7 TE
range2397987
1
398
417
annotation2397986
1
BBa_B0012
range2397986
1
391
431
annotation2397983
1
BBa_B0010
range2397983
1
303
382
annotation2397972
1
B1007
range2397972
1
152
191
annotation2397957
1
22
range2397957
1
1
10
annotation2397973
1
PolyA
range2397973
1
155
160
annotation2397968
1
PolyA
range2397968
1
108
112
annotation2397960
1
T7 TE
range2397960
1
8
27
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
false
true
_41_44_48_46_1_
0
24
7
Released HQ 2013
In stock
false
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
annotation1710
1
RBS
range1710
1
7
10
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation7027
1
BBa_B0032
range7027
1
1
13