//rbs/prokaryote/constitutive/miscellaneous
type: Collection
persistent identity: rbs prokaryote constitutive miscellaneous
display id: rbs_prokaryote_constitutive_miscellaneous
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_B0071
member: BBa_I742163
member: BBa_J59001
member: BBa_J64030
member: BBa_B0041
member: BBa_K118012
member: BBa_B0076
member: BBa_J34801
member: BBa_M13501
member: BBa_M13506
member: RBS-GsiB
member: BBa_I11010
member: BBa_B2017
member: BBa_B0037
member: BBa_J34810
member: BBa_J61143
member: BBa_B0039
member: creA RBS
member: BBa_K090506
member: BBa_I742145
member: BBa_J34803
member: BBa_M13503
member: BBa_J56016
member: T7 0.5
member: BBa_M13508
member: BBa_J64028
member: creD RBS
member: BBa_J64807
member: R37
member: BBa_Z0261
member: BBa_K090505
member: BBa_B2040
member: BBa_I723019
member: BBa_M13509
member: BBa_B2101
member: BBa_J61145
member: BBa_K082001
member: MBS
member: BBa_I742159
member: BBa_B2022
member: creB RBS
member: creC RBS
member: BBa_K103015
member: BBa_I723012
member: BBa_B0038
member: BBa_J61146
member: BBa_I742150
member: RBS
member: BBa_J61147
member: BBa_J64018
member: BBa_J64022
member: BBa_I742146
member: BBa_M13502
member: BBa_M13507
member: R38
member: BBa_Z0262
member: BBa_J64808
member: BBa_J15001
member: BBa_K150005
member: BBa_J56013
member: BBa_J64020
member: RBS-spoVG
member: BBa_B2102
member: BBa_B0036
member: BBa_I742153
member: BBa_B0070
member: BBa_J64015
member: BBa_K082000
member: BBa_I723014
member: BBa_J61144
member: BBa_J64024
member: BBa_J64011

RBS
type: ComponentDefinition
persistent identity: BBa J29048
display id: BBa_J29048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS
creator: Maiko Furubayashi
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _59_
m user id: 0
owner id: 846
owning group id: 59
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: strong rbs
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J29048_sequence/1

BBa_I742150
type: ComponentDefinition
persistent identity: BBa I742150
display id: BBa_I742150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtE native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site of crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321) Accession: D90087.
mutable notes: This is a virtual part to be added, blunt ended, to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321) Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742150_sequence/1

BBa_J61145
type: ComponentDefinition
persistent identity: BBa J61145
display id: BBa_J61145
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61145_sequence/1

BBa_M13502
type: ComponentDefinition
persistent identity: BBa M13502
display id: BBa_M13502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene II RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene II message (BBa_M13002). The part aligns with base pairs 8252-8267 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13502_sequence/1

BBa_M13507
type: ComponentDefinition
persistent identity: BBa M13507
display id: BBa_M13507
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene VII RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VII message (BBa_M13007). The part aligns with base pairs 1092-1107 in M13K07 genome. It was identified as the RBS for gene VII message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13507_sequence/1

BBa_B0041
type: ComponentDefinition
persistent identity: BBa B0041
display id: BBa_B0041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS 3
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0041_sequence/1

BBa_M13504
type: ComponentDefinition
persistent identity: BBa M13504
display id: BBa_M13504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene IV RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IV message (BBa_M13004). The part aligns with base pairs 4204-4219 in M13K07 genome. It was identified as the RBS for gene IV message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13504_sequence/1

BBa_J64030
type: ComponentDefinition
persistent identity: BBa J64030
display id: BBa_J64030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopD RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS from the sopD gene in Salmonella Type III Secretion.
mutable notes: This part was designed without consideration for biobrick standard assembly. This RBS has not been explicitly tested for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopD RBS
sequence: https://synbiohub.org/public/igem/BBa_J64030_sequence/1

sopD RBS
type: SequenceAnnotation
persistent identity: annotation1959634
display id: annotation1959634
version: 1
top level: BBa_J64030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64030/annotation1959634/range1959634/1

https://synbiohub.org/public/igem/BBa_J64030/annotation1959634/range1959634/1
type: Range
persistent identity: range1959634
display id: range1959634
version: 1
direction: direction/forward
top level: BBa_J64030
start: 1
end: 17
orientation: inline

BBa_B2017
type: ComponentDefinition
persistent identity: BBa B2017
display id: BBa_B2017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 2.8 RBS from T7
creator: Bartholomew Canton
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added automatically later
mutable notes: to be added automatically later
mutable provenance: to be added automatically later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B2017_sequence/1

BBa_J64011
type: ComponentDefinition
persistent identity: BBa J64011
display id: BBa_J64011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invB RBS
creator: Dan Widmaier
created: 2008-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The natural RBS that drives invB
mutable notes: The expression level of this RBS has not been quantified.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invB RBS
sequence: https://synbiohub.org/public/igem/BBa_J64011_sequence/1

invB RBS
type: SequenceAnnotation
persistent identity: annotation1959604
display id: annotation1959604
version: 1
top level: BBa_J64011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64011/annotation1959604/range1959604/1

https://synbiohub.org/public/igem/BBa_J64011/annotation1959604/range1959604/1
type: Range
persistent identity: range1959604
display id: range1959604
version: 1
direction: direction/forward
top level: BBa_J64011
start: 1
end: 19
orientation: inline

creD RBS
type: ComponentDefinition
persistent identity: BBa J64609
display id: BBa_J64609
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: creD RBS
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ribosomal binding site of the creD gene in the E Coli creD operon.
mutable notes: natural sequence, no design.
mutable provenance: E Coli Strain K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64609_sequence/1

BBa_K118012
type: ComponentDefinition
persistent identity: BBa K118012
display id: BBa_K118012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Synthetic ribosome binding site added by Son of Babel procedure
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a 4bp synthetic ribosome binding site which can be added by fusion PCR using a reverse primer and the Son of Babel cloning system.
mutable notes: The part can be added by fusion PCR using a Son of Babel reverse primer.
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K118012_sequence/1

BBa_K082000
type: ComponentDefinition
persistent identity: BBa K082000
display id: BBa_K082000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: reconstruct strong RBS
creator: Yanting Xue , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034.
mutable notes: The
mutable provenance: The
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K082000
sequence: https://synbiohub.org/public/igem/BBa_K082000_sequence/1

BBa_K082000
type: SequenceAnnotation
persistent identity: annotation1992768
display id: annotation1992768
version: 1
top level: BBa_K082000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082000/annotation1992768/range1992768/1

https://synbiohub.org/public/igem/BBa_K082000/annotation1992768/range1992768/1
type: Range
persistent identity: range1992768
display id: range1992768
version: 1
direction: direction/reverse
top level: BBa_K082000
start: 1
end: 17
orientation: inline

BBa_M13503
type: ComponentDefinition
persistent identity: BBa M13503
display id: BBa_M13503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene III RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene III message (BBa_M13003). The part aligns with base pairs 1563-1578 in M13K07 genome. It was identified as the RBS for gene III message by Wezenbeek et al in Gene (1980) 11: 129-148 New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13503_sequence/1

BBa_J61142
type: ComponentDefinition
persistent identity: BBa J61142
display id: BBa_J61142
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61142_sequence/1

BBa_I742156
type: ComponentDefinition
persistent identity: BBa I742156
display id: BBa_I742156
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtZ native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: A virtual part to be added to the coding sequence blunt (without a scar).
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742156_sequence/1

BBa_K150005
type: ComponentDefinition
persistent identity: BBa K150005
display id: BBa_K150005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site of pTrc99a
creator: Pascal Kraemer
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site of pTrc99a
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K150005
sequence: https://synbiohub.org/public/igem/BBa_K150005_sequence/1

BBa_K150005
type: SequenceAnnotation
persistent identity: annotation1983190
display id: annotation1983190
version: 1
top level: BBa_K150005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1

https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1
type: Range
persistent identity: range1983190
display id: range1983190
version: 1
direction: direction/forward
top level: BBa_K150005
start: 1
end: 13
orientation: inline

BBa_J56013
type: ComponentDefinition
persistent identity: BBa J56013
display id: BBa_J56013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs2 ribosome binding site
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site for transcription initiation
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J56013_sequence/1

BBa_K082001
type: ComponentDefinition
persistent identity: BBa K082001
display id: BBa_K082001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS medium
creator: Yanting Xue , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0030, BBa_B0031, BBa_B0033.
mutable notes: the
mutable provenance: the
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K082001
sequence: https://synbiohub.org/public/igem/BBa_K082001_sequence/1

BBa_K082001
type: SequenceAnnotation
persistent identity: annotation1993438
display id: annotation1993438
version: 1
top level: BBa_K082001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082001/annotation1993438/range1993438/1

https://synbiohub.org/public/igem/BBa_K082001/annotation1993438/range1993438/1
type: Range
persistent identity: range1993438
display id: range1993438
version: 1
direction: direction/reverse
top level: BBa_K082001
start: 1
end: 15
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong synthetic E. coli ribosome binding site with SacI site.
creator: Chris French
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
mutable provenance: Synthetic.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
direction: direction/forward
top level: BBa_J15001
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
direction: direction/forward
top level: BBa_J15001
start: 4
end: 10
orientation: inline

BBa_I723012
type: ComponentDefinition
persistent identity: BBa I723012
display id: BBa_I723012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Estimated RBS for DntR
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring RBS found in the regulatory region of the DntR gene.
mutable notes: '''''This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR'''''. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
mutable provenance: Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS for DntR
sequence: https://synbiohub.org/public/igem/BBa_I723012_sequence/1

RBS for DntR
type: SequenceAnnotation
persistent identity: annotation1954958
display id: annotation1954958
version: 1
top level: BBa_I723012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I723012/annotation1954958/range1954958/1

https://synbiohub.org/public/igem/BBa_I723012/annotation1954958/range1954958/1
type: Range
persistent identity: range1954958
display id: range1954958
version: 1
direction: direction/forward
top level: BBa_I723012
start: 1
end: 24
orientation: inline

BBa_I742159
type: ComponentDefinition
persistent identity: BBa I742159
display id: BBa_I742159
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtI native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native rbs from crtI (phytoene dehydrogenase) of Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: Virtual part to be added to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742159_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1953816
display id: annotation1953816
version: 1
top level: BBa_I742159
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742159/annotation1953816/range1953816/1

https://synbiohub.org/public/igem/BBa_I742159/annotation1953816/range1953816/1
type: Range
persistent identity: range1953816
display id: range1953816
version: 1
direction: direction/forward
top level: BBa_I742159
start: 10
end: 15
orientation: inline

BBa_B0036
type: ComponentDefinition
persistent identity: BBa B0036
display id: BBa_B0036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Barry Canton
created: 2004-06-07T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is recognized by ribosomes carrying the modified anti-Shine Dalgarno sequence as found in Brink et al. (see below)
mutable provenance: Brink MF, Verbeet MP, de Boer HA (1995). Specialized ribosomes: highly specific translation in vivo of a single targetted mRNA species. Gene, 156, pp. 215-222
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0036_sequence/1

BBa_B0070
type: ComponentDefinition
persistent identity: BBa B0070
display id: BBa_B0070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS modified from that of B0036 (Brink et al.)
creator: Barry Canton
created: 2006-03-03T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS has the modified Shine-Dalgarno sequence, it has a reduced secondary structure relative to using the actual Brink sequence with mixed connective sites flanking it. the spacing between the SD and the ATG is the same as that used by Brink et al. even though it is non-optimal for E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0070_sequence/1

BBa_J34803
type: ComponentDefinition
persistent identity: BBa J34803
display id: BBa_J34803
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34803_sequence/1

BBa_J64031
type: ComponentDefinition
persistent identity: BBa J64031
display id: BBa_J64031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sptP RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS for the sptP gene in Salmonella Type III Secretion
mutable notes: This part was designed without consideration for biobrick standard assembly. This RBS has not been explicitly tested for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sptP RBS
sequence: https://synbiohub.org/public/igem/BBa_J64031_sequence/1

sptP RBS
type: SequenceAnnotation
persistent identity: annotation1959635
display id: annotation1959635
version: 1
top level: BBa_J64031
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64031/annotation1959635/range1959635/1

https://synbiohub.org/public/igem/BBa_J64031/annotation1959635/range1959635/1
type: Range
persistent identity: range1959635
display id: range1959635
version: 1
direction: direction/forward
top level: BBa_J64031
start: 1
end: 20
orientation: inline

creB RBS
type: ComponentDefinition
persistent identity: BBa J64968
display id: BBa_J64968
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS on creB
creator: Kristopher Kuchenbecker
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ribosome Binding Site
mutable notes: RBS
mutable provenance: ''E. coli'' K-12 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64968_sequence/1

BBa_J61146
type: ComponentDefinition
persistent identity: BBa J61146
display id: BBa_J61146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61146_sequence/1

BBa_J34810
type: ComponentDefinition
persistent identity: BBa J34810
display id: BBa_J34810
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34810_sequence/1

BBa_B0077
type: ComponentDefinition
persistent identity: BBa B0077
display id: BBa_B0077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0077_sequence/1

BBa_I742153
type: ComponentDefinition
persistent identity: BBa I742153
display id: BBa_I742153
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtY native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site of crtY (lycopene cyclase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: This is a virtual part to be added, blunt-ended, to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742153_sequence/1

R38
type: ComponentDefinition
persistent identity: BBa K780001
display id: BBa_K780001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong RBS for Bacillus Subtilis
creator: Krzysztof Szczepaniak
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10271
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Natural RBS sequence for Bacillus Subtilis, described in the literature as a strong RBS for this bacteria.
mutable notes: -
mutable provenance: From genomic sequence Bacillus Subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K780001_sequence/1

T7 0.4
type: ComponentDefinition
persistent identity: BBa B2002
display id: BBa_B2002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.4
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the -20 to -1 region of T7 gp0.7, which contains the native RBS region for this gene. The physical instantiation of this part does not exist. This part is not compatible with Standard Biobricks assembly methods, as the scar created during assembly would interrupt the spacing between the RBS and the start codon of the CDS. Individuals wishing to use this RBS sequence in their assembles should refer to part XXXXX. (Information forthcoming)
mutable notes: The -20 to -1 region was chosen to be representative of the RBS region of the T7 genes for the following reasons: 1) The Shine-Delgarno region is contained within this defined region for all T7 genes. 2) Current literature suggests that when bound to the Translation Start Site (0), the Ribosome covers positions -20 to +13.
mutable provenance: Genome of wild-type bacteriophage T7, positions 2001-2020. Includes the -20 to -1 region of gp0.7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2002_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902419
display id: annotation1902419
version: 1
top level: T7 0.4
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B2002/annotation1902419/range1902419/1

https://synbiohub.org/public/igem/BBa_B2002/annotation1902419/range1902419/1
type: Range
persistent identity: range1902419
display id: range1902419
version: 1
direction: direction/forward
top level: T7 0.4
start: 4
end: 10
orientation: inline

BBa_Z0261
type: ComponentDefinition
persistent identity: BBa Z0261
display id: BBa_Z0261
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong T7.2 RBS
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Sequence comes from the natural gene 5 RBS. Using gene 5 as a strong RBS is from initial results from experiments characterizing all natural T7 RBSs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_Z0261_sequence/1

BBa_M13508
type: ComponentDefinition
persistent identity: BBa M13508
display id: BBa_M13508
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene VIII RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VIII message (BBa_M13008). The part aligns with base pairs 1285-1300 in M13K07 genome. It was identified as the RBS for gene VIII message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13508_sequence/1

BBa_M13501
type: ComponentDefinition
persistent identity: BBa M13501
display id: BBa_M13501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene I RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene I message (BBa_M13001). The part aligns with base pairs 8252-8267 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13501_sequence/1

BBa_B0039
type: ComponentDefinition
persistent identity: BBa B0039
display id: BBa_B0039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS 2
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0039_sequence/1

BBa_J64807
type: ComponentDefinition
persistent identity: BBa J64807
display id: BBa_J64807
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RocD RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RocD RBS
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64807_sequence/1

BBa_J61140
type: ComponentDefinition
persistent identity: BBa J61140
display id: BBa_J61140
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61140_sequence/1

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
creator: Todd Eckdahl
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Reverse version of RBS BBa_B0030
mutable notes: Repeats in oligos caused unusual products during cloning
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
direction: direction/reverse
top level: BBa_J44001
start: 1
end: 15
orientation: inline

BBa_J61143
type: ComponentDefinition
persistent identity: BBa J61143
display id: BBa_J61143
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61143_sequence/1

BBa_J64024
type: ComponentDefinition
persistent identity: BBa J64024
display id: BBa_J64024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sipC RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS from the sipC protein in Salmonella Type III Secretion.
mutable notes: This part was not designed with biobrick standard assembly in mind. This RBS has not been explicitly measured for expression rate using fluorescence or B-Gal assay.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sipC RBS
sequence: https://synbiohub.org/public/igem/BBa_J64024_sequence/1

sipC RBS
type: SequenceAnnotation
persistent identity: annotation1959623
display id: annotation1959623
version: 1
top level: BBa_J64024
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64024/annotation1959623/range1959623/1

https://synbiohub.org/public/igem/BBa_J64024/annotation1959623/range1959623/1
type: Range
persistent identity: range1959623
display id: range1959623
version: 1
direction: direction/forward
top level: BBa_J64024
start: 1
end: 15
orientation: inline

BBa_J64020
type: ComponentDefinition
persistent identity: BBa J64020
display id: BBa_J64020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invI RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural invI RBS from Salmonella Typhimurium LT2.
mutable notes: This part was not designed with consideration for biobrick standard assembly in mind. This RBS has not been explicitly tested for expression level in a fluorescence or B-Gal assay.
mutable provenance: Salmonella Typhimurium LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invI RBS
sequence: https://synbiohub.org/public/igem/BBa_J64020_sequence/1

invI RBS
type: SequenceAnnotation
persistent identity: annotation1959619
display id: annotation1959619
version: 1
top level: BBa_J64020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64020/annotation1959619/range1959619/1

https://synbiohub.org/public/igem/BBa_J64020/annotation1959619/range1959619/1
type: Range
persistent identity: range1959619
display id: range1959619
version: 1
direction: direction/forward
top level: BBa_J64020
start: 1
end: 20
orientation: inline

BBa_J64808
type: ComponentDefinition
persistent identity: BBa J64808
display id: BBa_J64808
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RocE RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS preceding RocE
mutable notes: n
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64808_sequence/1

BBa_J64013
type: ComponentDefinition
persistent identity: BBa J64013
display id: BBa_J64013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sipA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS that drives the sipA effector protein in the Salmonella SPI-1 system
mutable notes: This part was not designed with compatibility to biobrick standard assembly in mind. This RBS has not been explicitly tested in an expression assasy such as B-Gal or GFP expression.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sicA RBS
sequence: https://synbiohub.org/public/igem/BBa_J64013_sequence/1

sicA RBS
type: SequenceAnnotation
persistent identity: annotation1959612
display id: annotation1959612
version: 1
top level: BBa_J64013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64013/annotation1959612/range1959612/1

https://synbiohub.org/public/igem/BBa_J64013/annotation1959612/range1959612/1
type: Range
persistent identity: range1959612
display id: range1959612
version: 1
direction: direction/forward
top level: BBa_J64013
start: 1
end: 18
orientation: inline

BBa_J59001
type: ComponentDefinition
persistent identity: BBa J59001
display id: BBa_J59001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tuba
creator: Fatos tuba
created: 2006-08-28T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _93_
m user id: 0
owner id: 1162
owning group id: 93
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: tuba
sequence: https://synbiohub.org/public/igem/BBa_J59001_sequence/1

tuba
type: SequenceAnnotation
persistent identity: annotation1898348
display id: annotation1898348
version: 1
top level: BBa_J59001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J59001/annotation1898348/range1898348/1

https://synbiohub.org/public/igem/BBa_J59001/annotation1898348/range1898348/1
type: Range
persistent identity: range1898348
display id: range1898348
version: 1
direction: direction/reverse
top level: BBa_J59001
start: 1
end: 25
orientation: inline

BBa_I723014
type: ComponentDefinition
persistent identity: BBa I723014
display id: BBa_I723014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Estimated RBS for DntA
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring RBS found in the regulatory region of the DntA gene.
mutable notes: '''''This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR'''''. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
mutable provenance: Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_I723014_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation1954961
display id: annotation1954961
version: 1
top level: BBa_I723014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I723014/annotation1954961/range1954961/1

https://synbiohub.org/public/igem/BBa_I723014/annotation1954961/range1954961/1
type: Range
persistent identity: range1954961
display id: range1954961
version: 1
direction: direction/forward
top level: BBa_I723014
start: 1
end: 25
orientation: inline

BBa_M13511
type: ComponentDefinition
persistent identity: BBa M13511
display id: BBa_M13511
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene XI RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene XI message (BBa_M13011). The part aligns with base pairs 3900-3915 in M13K07 genome. It was NOT identified as the RBS for gene XI message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13511_sequence/1

MBS
type: ComponentDefinition
persistent identity: BBa J26002
display id: BBa_J26002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mario Binding Site
creator: Christian Jacob (hijacked by Patrick King)
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _77_
m user id: 0
owner id: 688
owning group id: 77
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J26002_sequence/1

BBa_B0078
type: ComponentDefinition
persistent identity: BBa B0078
display id: BBa_B0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0078_sequence/1

creA RBS
type: ComponentDefinition
persistent identity: BBa J64907
display id: BBa_J64907
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: creA RBS
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site for creA gene in the E Coli cre operon
mutable notes: natural sequence, no design.
mutable provenance: E Coli strain K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64907_sequence/1

BBa_J64028
type: ComponentDefinition
persistent identity: BBa J64028
display id: BBa_J64028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopB RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS for the sopB gene in the Salmonella Type III Secretion System.
mutable notes: This part was not designed with consideration for biobrick standard assembly in mind. This RBS hasn't been explicitly tested for expression rate in a Fluorescence or B-Gal assay.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopB RBS
sequence: https://synbiohub.org/public/igem/BBa_J64028_sequence/1

sopB RBS
type: SequenceAnnotation
persistent identity: annotation1959632
display id: annotation1959632
version: 1
top level: BBa_J64028
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64028/annotation1959632/range1959632/1

https://synbiohub.org/public/igem/BBa_J64028/annotation1959632/range1959632/1
type: Range
persistent identity: range1959632
display id: range1959632
version: 1
direction: direction/forward
top level: BBa_J64028
start: 1
end: 20
orientation: inline

BBa_I742145
type: ComponentDefinition
persistent identity: BBa I742145
display id: BBa_I742145
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam5 native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site for sam5 (4-coumarate-3-hydroxylase) of the bacterium Saccharothrix espanaensis.
mutable notes: This is a virtual part to be added to coding sequence I742141.
mutable provenance: Saccharothrix espanaensis DSM 44229
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742145_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1955475
display id: annotation1955475
version: 1
top level: BBa_I742145
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1

https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1
type: Range
persistent identity: range1955475
display id: range1955475
version: 1
direction: direction/forward
top level: BBa_I742145
start: 3
end: 9
orientation: inline

BBa_B2102
type: ComponentDefinition
persistent identity: BBa B2102
display id: BBa_B2102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.4 + SapI (rev)
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Insert Text here
mutable notes: Insert Text here
mutable provenance: Insert Text here
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BBa_B0079
sequence annotation: SD region
sequence annotation: BBa_B2002
sequence annotation: SapI
sequence: https://synbiohub.org/public/igem/BBa_B2102_sequence/1

BBa_B0079
type: SequenceAnnotation
persistent identity: annotation1902501
display id: annotation1902501
version: 1
top level: BBa_B2102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902501/range1902501/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902501/range1902501/1
type: Range
persistent identity: range1902501
display id: range1902501
version: 1
direction: direction/forward
top level: BBa_B2102
start: 21
end: 32
orientation: inline

SD region
type: SequenceAnnotation
persistent identity: annotation1902500
display id: annotation1902500
version: 1
top level: BBa_B2102
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902500/range1902500/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902500/range1902500/1
type: Range
persistent identity: range1902500
display id: range1902500
version: 1
direction: direction/forward
top level: BBa_B2102
start: 4
end: 10
orientation: inline

BBa_B2002
type: SequenceAnnotation
persistent identity: annotation1902499
display id: annotation1902499
version: 1
top level: BBa_B2102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902499/range1902499/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902499/range1902499/1
type: Range
persistent identity: range1902499
display id: range1902499
version: 1
direction: direction/forward
top level: BBa_B2102
start: 1
end: 20
orientation: inline

SapI
type: SequenceAnnotation
persistent identity: annotation1902502
display id: annotation1902502
version: 1
top level: BBa_B2102
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902502/range1902502/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902502/range1902502/1
type: Range
persistent identity: range1902502
display id: range1902502
version: 1
direction: direction/reverse
top level: BBa_B2102
start: 25
end: 31
orientation: inline

BBa_J56016
type: ComponentDefinition
persistent identity: BBa J56016
display id: BBa_J56016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-orig - ribosome binding site
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ribosome binding site for transcription initiation
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J56016_sequence/1

BBa_I742146
type: ComponentDefinition
persistent identity: BBa I742146
display id: BBa_I742146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam8 native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site of sam8 (tyrosine ammonia lyase) from the bacterium Saccharothrix espanaensis DSM 44229.
mutable notes: Virtual part to be added to coding sequence I742142.
mutable provenance: Saccharothrix espanaensis DSM 44229.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742146_sequence/1

BBa_M13509
type: ComponentDefinition
persistent identity: BBa M13509
display id: BBa_M13509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13Ko7 gene IX RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IX message (BBa_M13009). The part aligns with base pairs 1190-1205 in M13K07 genome. It was identified as the RBS for gene IX message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13509_sequence/1

T7 0.5
type: ComponentDefinition
persistent identity: BBa B2003
display id: BBa_B2003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.5
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the -20 to -1 region of T7 gp0.5, which contains the native RBS region for this gene. The physical instantiation of this part does not exist. This part is not compatible with Standard Biobricks assembly methods, as the scar created during assembly would interrupt the spacing between the RBS and the start codon of the CDS. Individuals wishing to use this RBS sequence in their assembles should refer to part B2103.
mutable notes: The -20 to -1 region was chosen to be representative of the RBS region of the T7 genes for the following reasons: 1) The Shine-Delgarno region is contained within this defined region for all T7 genes. 2) Current literature suggests that when bound to the Translation Start Site (0), the Ribosome covers positions -20 to +13 of the gene.
mutable provenance: Genome of wild-type bacteriophage T7, positions 1476-1495. Includes the -20 to -1 region of gp0.5.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2003_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902509
display id: annotation1902509
version: 1
top level: T7 0.5
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2003/annotation1902509/range1902509/1

https://synbiohub.org/public/igem/BBa_B2003/annotation1902509/range1902509/1
type: Range
persistent identity: range1902509
display id: range1902509
version: 1
direction: direction/forward
top level: T7 0.5
start: 13
end: 18
orientation: inline

BBa_I723019
type: ComponentDefinition
persistent identity: BBa I723019
display id: BBa_I723019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS for XylR
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring RBS found in the regulatory region of the DntR gene. gataaaaacaagaggaaaacaa
mutable notes: [Chistine/Maia]
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_I723019_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1955263
display id: annotation1955263
version: 1
top level: BBa_I723019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I723019/annotation1955263/range1955263/1

https://synbiohub.org/public/igem/BBa_I723019/annotation1955263/range1955263/1
type: Range
persistent identity: range1955263
display id: range1955263
version: 1
direction: direction/forward
top level: BBa_I723019
start: 1
end: 20
orientation: inline

BBa_B0038
type: ComponentDefinition
persistent identity: BBa B0038
display id: BBa_B0038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS 1
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0038_sequence/1

BBa_J64018
type: ComponentDefinition
persistent identity: BBa J64018
display id: BBa_J64018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invJ RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS associated with the invJ protein in Salmonella Typhimurium LT2.
mutable notes: This part was not designed with compliance to biobrick standard assembly in mind.
mutable provenance: Salmonella Typhimurium LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invJ RBS
sequence: https://synbiohub.org/public/igem/BBa_J64018_sequence/1

invJ RBS
type: SequenceAnnotation
persistent identity: annotation1959617
display id: annotation1959617
version: 1
top level: BBa_J64018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64018/annotation1959617/range1959617/1

https://synbiohub.org/public/igem/BBa_J64018/annotation1959617/range1959617/1
type: Range
persistent identity: range1959617
display id: range1959617
version: 1
direction: direction/forward
top level: BBa_J64018
start: 1
end: 17
orientation: inline

BBa_B2022
type: ComponentDefinition
persistent identity: BBa B2022
display id: BBa_B2022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 4B RBS from T7
creator: Bartholomew Canton
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added automatically later
mutable notes: to be added automatically later
mutable provenance: to be added automatically later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B2022_sequence/1

BBa_J64026
type: ComponentDefinition
persistent identity: BBa J64026
display id: BBa_J64026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sigE RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS for the sigE chaperone (BBa_J64025) in Salmonella Typhimurium LT2.
mutable notes: This part was not designed with compatibility to biobrick standard assembly in mind. This RBS has not been explicitly assayed for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sigE RBS
sequence: https://synbiohub.org/public/igem/BBa_J64026_sequence/1

sigE RBS
type: SequenceAnnotation
persistent identity: annotation1959625
display id: annotation1959625
version: 1
top level: BBa_J64026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64026/annotation1959625/range1959625/1

https://synbiohub.org/public/igem/BBa_J64026/annotation1959625/range1959625/1
type: Range
persistent identity: range1959625
display id: range1959625
version: 1
direction: direction/forward
top level: BBa_J64026
start: 1
end: 18
orientation: inline

BBa_B0037
type: ComponentDefinition
persistent identity: BBa B0037
display id: BBa_B0037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Barry Canton
created: 2005-12-06T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS specific to dedicated ribosomes. This RBS is a modified version of B0036. The spacing between the SD sequence and the start codon has been increased by 5 bases to give the same spacing as used by Brink et al. The 5 bases are the same as those used by Brink. That means they form the first five bases of an XbaI site and no negative affects of using these bases are known at present.
mutable provenance: Brink MF, Verbeet MP, de Boer HA (1995). Specialized ribosomes: highly specific translation in vivo of a single targetted mRNA species. Gene, 156, pp. 215-222
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0037_sequence/1

BBa_I742163
type: ComponentDefinition
persistent identity: BBa I742163
display id: BBa_I742163
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: native ftsK rbs
creator: Chris French
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site of ftsK, a DNA-translocating motor protein involved in cell division in Escherichia coli.
mutable notes: Virtual part to be added to coding sequence BBa_I742137.
mutable provenance: Escherichia coli JM109 (a K12 strain).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742163_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1954640
display id: annotation1954640
version: 1
top level: BBa_I742163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I742163/annotation1954640/range1954640/1

https://synbiohub.org/public/igem/BBa_I742163/annotation1954640/range1954640/1
type: Range
persistent identity: range1954640
display id: range1954640
version: 1
direction: direction/forward
top level: BBa_I742163
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1954641
display id: annotation1954641
version: 1
top level: BBa_I742163
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742163/annotation1954641/range1954641/1

https://synbiohub.org/public/igem/BBa_I742163/annotation1954641/range1954641/1
type: Range
persistent identity: range1954641
display id: range1954641
version: 1
direction: direction/forward
top level: BBa_I742163
start: 15
end: 20
orientation: inline

BBa_J61147
type: ComponentDefinition
persistent identity: BBa J61147
display id: BBa_J61147
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61147_sequence/1

BBa_B2101
type: ComponentDefinition
persistent identity: BBa B2101
display id: BBa_B2101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.3 + SapI (rev)
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This version of the T7 0.3 RBS is designed for construction via Heather Keller's alternative assembly plan using XbaI and SapI for RBS/CDS assembly without a mixed site. Contains the -20 to -1 region of gp0.4 from the wild-type T7 genome. Can also be used with Standard BioBricks assembly, although this will add a 7 amino acid fusion to the N-terminus of the resulting protein.
mutable notes: This RBS is designed to be compatible with Heather Keller's RBS assembly scheme for creating RBS/CDS junctions without a mixed site. For use in this scheme, this part should be cut with XbaI and SapI. It must be used with a coding sequence containing the SapI site at the 5' end. This part is not compatible with CDSs that use a GTG site and must be used with CDSs using an ATG start site. The two bp spacer on the 3' end also allows this part to be used in standard biobricks assembly and is necessary to maintain the reading frame of a downstream CDS. Note that Standard Biobricks Assembly with this part results in the creation of a new ATG start site, and a 7 amino acid fusion at the N terminus of the downstream protein. CDSs with alternative start sites are compatible with this part via Standard Assembly. This part was inadvertently constructed without the single base pair (G/C) that separates the XbaI site and the 5' end of the part.
mutable provenance: The RBS is taken from the wild-type bacteriophage T7 genome. Please see part B2001 for more information.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_B2001
sequence annotation: BBa_B2001
sequence: https://synbiohub.org/public/igem/BBa_B2101_sequence/1

BBa_B2001
type: Component
persistent identity: component2218355
display id: component2218355
version: 1
top level: BBa_B2101
access: public
definition: T7 0.3

BBa_B2001
type: SequenceAnnotation
persistent identity: annotation2218355
display id: annotation2218355
version: 1
top level: BBa_B2101
location: https://synbiohub.org/public/igem/BBa_B2101/annotation2218355/range2218355/1
component: BBa_B2001

https://synbiohub.org/public/igem/BBa_B2101/annotation2218355/range2218355/1
type: Range
persistent identity: range2218355
display id: range2218355
version: 1
direction: direction/reverse
top level: BBa_B2101
start: 1
end: 20
orientation: inline

RBS-GsiB
type: ComponentDefinition
persistent identity: BBa K143020
display id: BBa_K143020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GsiB ribosome binding site (RBS) for B. subtilis
creator: James Chappell
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: GsiB is an endogenous ribosome binding site from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. GsiB is an endogenous ribosome binding site (RBS) from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -9.3kcal.
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143020_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975872
display id: annotation1975872
version: 1
top level: RBS-GsiB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1

https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1
type: Range
persistent identity: range1975872
display id: range1975872
version: 1
direction: direction/forward
top level: RBS-GsiB
start: 2
end: 8
orientation: inline

BBa_Z0262
type: ComponentDefinition
persistent identity: BBa Z0262
display id: BBa_Z0262
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium strength T7.2 RBS
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Sequence comes from the natural B0030. Using B0030 as a medium strength RBS is from initial results from experiments characterizing all natural T7 RBSs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_Z0262_sequence/1

BBa_J61141
type: ComponentDefinition
persistent identity: BBa J61141
display id: BBa_J61141
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61141_sequence/1

BBa_B2040
type: ComponentDefinition
persistent identity: BBa B2040
display id: BBa_B2040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 8 RBS from T7
creator: Bartholomew Canton
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added automatically later
mutable notes: to be added automatically later
mutable provenance: to be added automatically later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B2040_sequence/1

BBa_I11010
type: ComponentDefinition
persistent identity: BBa I11010
display id: BBa_I11010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS
creator: bu
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_I11010_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1147662
display id: annotation1147662
version: 1
top level: BBa_I11010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1

https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1
type: Range
persistent identity: range1147662
display id: range1147662
version: 1
direction: direction/forward
top level: BBa_I11010
start: 1
end: 6
orientation: inline

BBa_K103015
type: ComponentDefinition
persistent identity: BBa K103015
display id: BBa_K103015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: consensus RBS
creator: Michael Lower
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: strong RBS
sequence: https://synbiohub.org/public/igem/BBa_K103015_sequence/1

strong RBS
type: SequenceAnnotation
persistent identity: annotation1979577
display id: annotation1979577
version: 1
top level: BBa_K103015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K103015/annotation1979577/range1979577/1

https://synbiohub.org/public/igem/BBa_K103015/annotation1979577/range1979577/1
type: Range
persistent identity: range1979577
display id: range1979577
version: 1
direction: direction/forward
top level: BBa_K103015
start: 1
end: 6
orientation: inline

BBa_J64022
type: ComponentDefinition
persistent identity: BBa J64022
display id: BBa_J64022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sicA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS from the sicA chaperone (BBa_J6021) in the Salmonella Type III Secretion System.
mutable notes: This part was not designed with standard biobrick assembly in mind.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_J64022_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation1959621
display id: annotation1959621
version: 1
top level: BBa_J64022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1

https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1
type: Range
persistent identity: range1959621
display id: range1959621
version: 1
direction: direction/forward
top level: BBa_J64022
start: 1
end: 20
orientation: inline

T7 0.3
type: ComponentDefinition
persistent identity: BBa B2001
display id: BBa_B2001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.3
creator: Heather Keller
created: 2006-09-26T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Test RBS from T7 that may be used in E.coli
mutable notes: None
mutable provenance: T7+ genome RBS upstream of gene 0.3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2001_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902503
display id: annotation1902503
version: 1
top level: T7 0.3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2001/annotation1902503/range1902503/1

https://synbiohub.org/public/igem/BBa_B2001/annotation1902503/range1902503/1
type: Range
persistent identity: range1902503
display id: range1902503
version: 1
direction: direction/forward
top level: T7 0.3
start: 8
end: 12
orientation: inline

BBa_J64809
type: ComponentDefinition
persistent identity: BBa J64809
display id: BBa_J64809
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RocF RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS upstream of RocF
mutable notes: n
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64809_sequence/1

creC RBS
type: ComponentDefinition
persistent identity: BBa J64908
display id: BBa_J64908
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS for creC in e coli operon
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ribosomal binding site for gene creC in the E Coli cre operon.
mutable notes: natural sequence, no design
mutable provenance: E Coli K12 strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64908_sequence/1

BBa_M13510
type: ComponentDefinition
persistent identity: BBa M13510
display id: BBa_M13510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene X RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene X message (BBa_M13010). The part aligns with base pairs 480-495 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13510_sequence/1

BBa_J34801
type: ComponentDefinition
persistent identity: BBa J34801
display id: BBa_J34801
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34801_sequence/1

BBa_K090506
type: ComponentDefinition
persistent identity: BBa K090506
display id: BBa_K090506
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ''Bacillus subtilis'' weak RBS
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the weak version of the consensus RBS for Bacillus subtilis. The sequence is present in front of some B. subtilis operons, but is expected to give weaker expression than the consensus sequence. The consensus sequence AAAGGAGG (BBa_K090505) was changed to AGAGGTGG for this part.
mutable notes: The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AGAGGTGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
mutable provenance: This part was synthesized directly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: B. subtilis weak RBS
sequence: https://synbiohub.org/public/igem/BBa_K090506_sequence/1

B. subtilis weak RBS
type: SequenceAnnotation
persistent identity: annotation1992815
display id: annotation1992815
version: 1
top level: BBa_K090506
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K090506/annotation1992815/range1992815/1

https://synbiohub.org/public/igem/BBa_K090506/annotation1992815/range1992815/1
type: Range
persistent identity: range1992815
display id: range1992815
version: 1
direction: direction/forward
top level: BBa_K090506
start: 1
end: 8
orientation: inline

BBa_J61144
type: ComponentDefinition
persistent identity: BBa J61144
display id: BBa_J61144
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61144_sequence/1

BBa_J64015
type: ComponentDefinition
persistent identity: BBa J64015
display id: BBa_J64015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS associated with the sopA effector protein in the Salmonella SPI-1 system.
mutable notes: This part was not designed with compliance to biobrick standard assembly in mind. This RBS hasn't been explicitly tested in an expression assay such as GFP fluorescence or B-Gal expression.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopA RBS
sequence: https://synbiohub.org/public/igem/BBa_J64015_sequence/1

sopA RBS
type: SequenceAnnotation
persistent identity: annotation1959614
display id: annotation1959614
version: 1
top level: BBa_J64015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64015/annotation1959614/range1959614/1

https://synbiohub.org/public/igem/BBa_J64015/annotation1959614/range1959614/1
type: Range
persistent identity: range1959614
display id: range1959614
version: 1
direction: direction/forward
top level: BBa_J64015
start: 1
end: 16
orientation: inline

BBa_K090505
type: ComponentDefinition
persistent identity: BBa K090505
display id: BBa_K090505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ''Bacillus subtilis'' consensus RBS
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the consensus RBS for Bacillus subtilis.
mutable notes: The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AAAGGAGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
mutable provenance: This part was synthesized directly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: B. subtilis consensus RBS
sequence: https://synbiohub.org/public/igem/BBa_K090505_sequence/1

B. subtilis consensus RBS
type: SequenceAnnotation
persistent identity: annotation1992802
display id: annotation1992802
version: 1
top level: BBa_K090505
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K090505/annotation1992802/range1992802/1

https://synbiohub.org/public/igem/BBa_K090505/annotation1992802/range1992802/1
type: Range
persistent identity: range1992802
display id: range1992802
version: 1
direction: direction/forward
top level: BBa_K090505
start: 1
end: 8
orientation: inline

revRBS
type: ComponentDefinition
persistent identity: BBa I742130
display id: BBa_I742130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse RBS
creator: Caroline Dahl
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: DNA is double-stranded. To translate genes of both strands, you need RBSs in both strands.
mutable notes: Please refer to the original (BB_B0034).
mutable provenance: Please refer to the original (BB_B0034).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742130_sequence/1

R37
type: ComponentDefinition
persistent identity: BBa K780002
display id: BBa_K780002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong RBS for Bacillus Subtilis
creator: Krzysztof Szczepaniak
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong RBS for Bacillus Subtilis
mutable notes: -
mutable provenance: 'host-takover module' from B.subtilis Bacteriophage SPO1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K780002_sequence/1

BBa_M13506
type: ComponentDefinition
persistent identity: BBa M13506
display id: BBa_M13506
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene VI RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VI message (BBa_M13006). The part aligns with base pairs 2840-2855 in M13K07 genome. It was identified as the RBS for gene VI message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13506_sequence/1

BBa_M13505
type: ComponentDefinition
persistent identity: BBa M13505
display id: BBa_M13505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene V RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene V message (BBa_M13005). The part aligns with base pairs 827-842 in M13K07 genome. It was identified as the RBS for the gene V message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13505_sequence/1

BBa_B0076
type: ComponentDefinition
persistent identity: BBa B0076
display id: BBa_B0076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0076_sequence/1

BBa_B0071
type: ComponentDefinition
persistent identity: BBa B0071
display id: BBa_B0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Barry Canton
created: 2006-03-03T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS has the modified Shine-Dalgarno sequence, it has a reduced secondary structure relative to using the actual Brink sequence with mixed connective sites flanking it. This RBS is modified from B0071 by reducing the 3' sequence so that the SD to ATG spacing is closer to that which is optimal for E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0071_sequence/1

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpoVG ribosome binding site (RBS) for B. subtilis
creator: James Chappell
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
direction: direction/forward
top level: RBS-spoVG
start: 1
end: 12
orientation: inline