//regulation/negative
type: Collection
persistent identity: regulation negative
display id: regulation_negative
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: MicF
member: U073O11+D0
member: BBa_I766214
member: BBa_I12036
member: U097NUL+D0
member: BBa_K137051
member: U085O11+D0
member: Prm +
member: BBa_K389052
member: Pnot(O44)
member: BBa_K136004
member: U049O11+D0
member: BBa_K137046
member: BBa_R2113
member: pRecA
member: BBa_K1031743
member: BBa_K113009
member: BBa_K266007
member: BBa_J22126
member: Pxyl
member: LacI:Cpn60
member: BBa_K1886017
member: BBa_J54130
member: BBa_K112701
member: BBa_K1520005
member: BBa_K950003
member: LacI:Cpn10
member: BBa_K109200
member: BBa_K598020
member: BBa_K145150
member: Ph-s
member: BBa_K174001
member: Pnot(O26)
member: BBa_K174002
member: BBa_K1824895
member: cII p22
member: Pnot(O12)
member: BBa_J64069
member: U097O11+D0
member: BBa_I739101
member: BBa_K086032
member: NOR2
member: BBa_I756020
member: BBa_I739106
member: BBa_R0186
member: BBa_R2201
member: U037O11+D0
member: U061O11+D0
member: Pspac-hy
member: BBa_K112708
member: BBa_K091101
member: BBa_K091157
member: BBa_I714924
member: BBa_K266006
member: Zif23
member: BBa_K256028
member: BBa_K137050
member: BBa_I756014
member: BBa_I756019
member: BBa_K518008
member: BBa_K086026
member: BBa_K112402
member: Pnot(O46)
member: BBa_J15301
member: BBa_K190016
member: NAND59

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732446
display id: BBa_I732446
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732446_sequence/1

BBa_K1031743
type: ComponentDefinition
persistent identity: BBa K1031743
display id: BBa_K1031743
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-PaaX
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-PaaX-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_J23113
sequence annotation: BBa_B0034
sequence annotation: PaaX
sequence: https://synbiohub.org/public/igem/BBa_K1031743_sequence/1

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2357491
display id: annotation2357491
version: 1
top level: BBa_K1031743
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357491/range2357491/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357491/range2357491/1
type: Range
persistent identity: range2357491
display id: range2357491
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357492
display id: annotation2357492
version: 1
top level: BBa_K1031743
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357492/range2357492/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357492/range2357492/1
type: Range
persistent identity: range2357492
display id: range2357492
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 44
end: 55
orientation: inline

PaaX
type: SequenceAnnotation
persistent identity: annotation2357493
display id: annotation2357493
version: 1
top level: BBa_K1031743
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357493/range2357493/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357493/range2357493/1
type: Range
persistent identity: range2357493
display id: range2357493
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 62
end: 1012
orientation: inline

BBa_I739103
type: ComponentDefinition
persistent identity: BBa I739103
display id: BBa_I739103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (lacI, negative / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: R0053 (2x OR1)
sequence annotation: R0011
sequence: https://synbiohub.org/public/igem/BBa_I739103_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1947821
display id: annotation1947821
version: 1
top level: BBa_I739103
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947821/range1947821/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947821/range1947821/1
type: Range
persistent identity: range1947821
display id: range1947821
version: 1
direction: direction/forward
top level: BBa_I739103
start: 50
end: 68
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947822
display id: annotation1947822
version: 1
top level: BBa_I739103
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947822/range1947822/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947822/range1947822/1
type: Range
persistent identity: range1947822
display id: range1947822
version: 1
direction: direction/forward
top level: BBa_I739103
start: 69
end: 87
orientation: inline

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947823
display id: annotation1947823
version: 1
top level: BBa_I739103
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947823/range1947823/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947823/range1947823/1
type: Range
persistent identity: range1947823
display id: range1947823
version: 1
direction: direction/forward
top level: BBa_I739103
start: 50
end: 87
orientation: inline

R0011
type: SequenceAnnotation
persistent identity: annotation1947820
display id: annotation1947820
version: 1
top level: BBa_I739103
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947820/range1947820/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947820/range1947820/1
type: Range
persistent identity: range1947820
display id: range1947820
version: 1
direction: direction/forward
top level: BBa_I739103
start: 1
end: 48
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732423
display id: BBa_I732423
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732423_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732416
display id: BBa_I732416
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732416_sequence/1

Pnot(O17)
type: ComponentDefinition
persistent identity: BBa I732272
display id: BBa_I732272
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O17)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732272_sequence/1

BBa_K086022
type: ComponentDefinition
persistent identity: BBa K086022
display id: BBa_K086022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: Cleaned
mutable provenance: Lac and sigma 28 conserved binding sites
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P28_1
sequence: https://synbiohub.org/public/igem/BBa_K086022_sequence/1

P28_1
type: SequenceAnnotation
persistent identity: annotation1995756
display id: annotation1995756
version: 1
top level: BBa_K086022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086022/annotation1995756/range1995756/1

https://synbiohub.org/public/igem/BBa_K086022/annotation1995756/range1995756/1
type: Range
persistent identity: range1995756
display id: range1995756
version: 1
direction: direction/forward
top level: BBa_K086022
start: 1
end: 55
orientation: inline

Ph-s
type: ComponentDefinition
persistent identity: BBa K143015
display id: BBa_K143015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter hyper-spank for B. subtilis
creator: Chris Hirst
created: 2008-09-17T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter hyper-spank is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG). The context with which we used the promoter hyper-spank, was to take an input of IPTG and give '''Polymerase Per Second'''(PoPS) as an output. IPTG does not induce the promoter hyper-spank directly, but requires the transcriptional regulator '''LacI''', ( BBa_K413035 ). This means that LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank.
mutable notes: Biobrick standard was applied to the promoter hyper-spank sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 123. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A -10
sequence annotation: LacI Operator
sequence annotation: Sigma A -35
sequence: https://synbiohub.org/public/igem/BBa_K143015_sequence/1

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1976425
display id: annotation1976425
version: 1
top level: Ph-s
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976425/range1976425/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976425/range1976425/1
type: Range
persistent identity: range1976425
display id: range1976425
version: 1
direction: direction/forward
top level: Ph-s
start: 69
end: 74
orientation: inline

LacI Operator
type: SequenceAnnotation
persistent identity: annotation1976426
display id: annotation1976426
version: 1
top level: Ph-s
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976426/range1976426/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976426/range1976426/1
type: Range
persistent identity: range1976426
display id: range1976426
version: 1
direction: direction/forward
top level: Ph-s
start: 81
end: 101
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1976424
display id: annotation1976424
version: 1
top level: Ph-s
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976424/range1976424/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976424/range1976424/1
type: Range
persistent identity: range1976424
display id: range1976424
version: 1
direction: direction/forward
top level: Ph-s
start: 46
end: 50
orientation: inline

LacI Operator
type: SequenceAnnotation
persistent identity: annotation1976423
display id: annotation1976423
version: 1
top level: Ph-s
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976423/range1976423/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976423/range1976423/1
type: Range
persistent identity: range1976423
display id: range1976423
version: 1
direction: direction/forward
top level: Ph-s
start: 10
end: 30
orientation: inline

Pnot(O61)
type: ComponentDefinition
persistent identity: BBa I732279
display id: BBa_I732279
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O61)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732279_sequence/1

BBa_K256028
type: ComponentDefinition
persistent identity: BBa K256028
display id: BBa_K256028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: placI:CHE
creator: iGEM09_NTU-Singapore
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Generator of (CHE)Cholesterol esterase. Attached to placI, the part is inducible by IPTG and Lactose. It works better in Lactose.
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K256023
component: BBa_B0010
component: BBa_R0010
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K256023
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K256028_sequence/1

BBa_K256023
type: Component
persistent identity: component2052738
display id: component2052738
version: 1
top level: BBa_K256028
access: public
definition: https://synbiohub.org/public/igem/BBa_K256023/1

BBa_B0010
type: Component
persistent identity: component2052739
display id: component2052739
version: 1
top level: BBa_K256028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component2052729
display id: component2052729
version: 1
top level: BBa_K256028
access: public
definition: LacI

BBa_B0034
type: Component
persistent identity: component2052737
display id: component2052737
version: 1
top level: BBa_K256028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2052741
display id: component2052741
version: 1
top level: BBa_K256028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2052729
display id: annotation2052729
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052729/range2052729/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K256028/annotation2052729/range2052729/1
type: Range
persistent identity: range2052729
display id: range2052729
version: 1
direction: direction/forward
top level: BBa_K256028
start: 1
end: 200
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2052741
display id: annotation2052741
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052741/range2052741/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K256028/annotation2052741/range2052741/1
type: Range
persistent identity: range2052741
display id: range2052741
version: 1
direction: direction/forward
top level: BBa_K256028
start: 1265
end: 1305
orientation: inline

BBa_K256023
type: SequenceAnnotation
persistent identity: annotation2052738
display id: annotation2052738
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052738/range2052738/1
component: BBa_K256023

https://synbiohub.org/public/igem/BBa_K256028/annotation2052738/range2052738/1
type: Range
persistent identity: range2052738
display id: range2052738
version: 1
direction: direction/reverse
top level: BBa_K256028
start: 227
end: 1168
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2052737
display id: annotation2052737
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052737/range2052737/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256028/annotation2052737/range2052737/1
type: Range
persistent identity: range2052737
display id: range2052737
version: 1
direction: direction/reverse
top level: BBa_K256028
start: 209
end: 220
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2052739
display id: annotation2052739
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052739/range2052739/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K256028/annotation2052739/range2052739/1
type: Range
persistent identity: range2052739
display id: range2052739
version: 1
direction: direction/forward
top level: BBa_K256028
start: 1177
end: 1256
orientation: inline

BBa_K091107
type: ComponentDefinition
persistent identity: BBa K091107
display id: BBa_K091107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/cI Hybrid Promoter
creator: Xiao Zhu
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This hybrid promoter is designed to respond to activation by LuxR when it is bound to AHL and to repression by the phage lambda cI repressor. The repression needs to win over activation.
mutable notes: This part is based on the idea of replacing the region of the Lux promoter downstream of the -35 with the OR2 cI half-operator, added LuxR-10 region. Based on what we have found up to now, promoters which are constructed just by inserting OR1/2 downstream of Lux are all very leaky.
mutable provenance: this part comes from LuxR promoter and cI lambda promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lux -10
sequence annotation: cI OR2
sequence annotation: Lux Box
sequence annotation: Lux -35
sequence: https://synbiohub.org/public/igem/BBa_K091107_sequence/1

Lux -10
type: SequenceAnnotation
persistent identity: annotation1963753
display id: annotation1963753
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1
type: Range
persistent identity: range1963753
display id: range1963753
version: 1
direction: direction/forward
top level: BBa_K091107
start: 44
end: 49
orientation: inline

cI OR2
type: SequenceAnnotation
persistent identity: annotation1963752
display id: annotation1963752
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1
type: Range
persistent identity: range1963752
display id: range1963752
version: 1
direction: direction/forward
top level: BBa_K091107
start: 26
end: 43
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1963750
display id: annotation1963750
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1
type: Range
persistent identity: range1963750
display id: range1963750
version: 1
direction: direction/forward
top level: BBa_K091107
start: 1
end: 20
orientation: inline

Lux -35
type: SequenceAnnotation
persistent identity: annotation1963751
display id: annotation1963751
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1
type: Range
persistent identity: range1963751
display id: range1963751
version: 1
direction: direction/forward
top level: BBa_K091107
start: 20
end: 25
orientation: inline

BBa_K174002
type: ComponentDefinition
persistent identity: BBa K174002
display id: BBa_K174002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose controlled protein degradation device
creator: The Newcastle 2009 iGEM team
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:10:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _277_
m user id: 0
owner id: 3942
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The purpose of this system is to control the expression of sspB, the degradation controller. The system is inducible by arabinose, therefore we can alter the arabinose levels to regulate the degradation of proteins with sspB degradation tag
mutable notes: sspB Biobrick is inserted after araR promoter+rbs system
mutable provenance: Comprised of other Biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K174001
component: BBa_K174000
sequence annotation: BBa_K174001
sequence annotation: BBa_K174000
sequence: https://synbiohub.org/public/igem/BBa_K174002_sequence/1

BBa_K174001
type: Component
persistent identity: component2027244
display id: component2027244
version: 1
top level: BBa_K174002
access: public
definition: BBa_K174001

BBa_K174000
type: Component
persistent identity: component2027246
display id: component2027246
version: 1
top level: BBa_K174002
access: public
definition: https://synbiohub.org/public/igem/BBa_K174000/1

BBa_K174001
type: SequenceAnnotation
persistent identity: annotation2027244
display id: annotation2027244
version: 1
top level: BBa_K174002
location: https://synbiohub.org/public/igem/BBa_K174002/annotation2027244/range2027244/1
component: BBa_K174001

https://synbiohub.org/public/igem/BBa_K174002/annotation2027244/range2027244/1
type: Range
persistent identity: range2027244
display id: range2027244
version: 1
direction: direction/reverse
top level: BBa_K174002
start: 1
end: 215
orientation: inline

BBa_K174000
type: SequenceAnnotation
persistent identity: annotation2027246
display id: annotation2027246
version: 1
top level: BBa_K174002
location: https://synbiohub.org/public/igem/BBa_K174002/annotation2027246/range2027246/1
component: BBa_K174000

https://synbiohub.org/public/igem/BBa_K174002/annotation2027246/range2027246/1
type: Range
persistent identity: range2027246
display id: range2027246
version: 1
direction: direction/reverse
top level: BBa_K174002
start: 222
end: 719
orientation: inline

BBa_R0187
type: ComponentDefinition
persistent identity: BBa R0187
display id: BBa_R0187
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an G, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_R0187_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1962097
display id: annotation1962097
version: 1
top level: BBa_R0187
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0187/annotation1962097/range1962097/1

https://synbiohub.org/public/igem/BBa_R0187/annotation1962097/range1962097/1
type: Range
persistent identity: range1962097
display id: range1962097
version: 1
direction: direction/forward
top level: BBa_R0187
start: 1
end: 20
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962096
display id: annotation1962096
version: 1
top level: BBa_R0187
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0187/annotation1962096/range1962096/1

https://synbiohub.org/public/igem/BBa_R0187/annotation1962096/range1962096/1
type: Range
persistent identity: range1962096
display id: range1962096
version: 1
direction: direction/forward
top level: BBa_R0187
start: 20
end: 44
orientation: inline

BBa_K091101
type: ComponentDefinition
persistent identity: BBa K091101
display id: BBa_K091101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet_Lac hybrid promoter
creator: Erin Feeney
created: 2008-05-28T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter is repressed by tetR and lacI; it contains two binding sites for TetR and one LacI binding site. this part is an AND-gate since transcription occurs only in the presence of both ATC and IPTG.
mutable notes: Also designed by Pallavi Penumetcha and Robert Cool.
mutable provenance: This part consists of the R0040 tetR promoter with the addition of the R0010 lacI binding site.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: tetR binding site 1
sequence annotation: -35
sequence annotation: tetR binding site 2
sequence annotation: lacI binding site
sequence: https://synbiohub.org/public/igem/BBa_K091101_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1963340
display id: annotation1963340
version: 1
top level: BBa_K091101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963340/range1963340/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963340/range1963340/1
type: Range
persistent identity: range1963340
display id: range1963340
version: 1
direction: direction/forward
top level: BBa_K091101
start: 43
end: 48
orientation: inline

tetR binding site 1
type: SequenceAnnotation
persistent identity: annotation1963337
display id: annotation1963337
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963337/range1963337/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963337/range1963337/1
type: Range
persistent identity: range1963337
display id: range1963337
version: 1
direction: direction/forward
top level: BBa_K091101
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1963339
display id: annotation1963339
version: 1
top level: BBa_K091101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963339/range1963339/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963339/range1963339/1
type: Range
persistent identity: range1963339
display id: range1963339
version: 1
direction: direction/forward
top level: BBa_K091101
start: 20
end: 25
orientation: inline

tetR binding site 2
type: SequenceAnnotation
persistent identity: annotation1963338
display id: annotation1963338
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963338/range1963338/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963338/range1963338/1
type: Range
persistent identity: range1963338
display id: range1963338
version: 1
direction: direction/forward
top level: BBa_K091101
start: 26
end: 44
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1963341
display id: annotation1963341
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963341/range1963341/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963341/range1963341/1
type: Range
persistent identity: range1963341
display id: range1963341
version: 1
direction: direction/forward
top level: BBa_K091101
start: 49
end: 83
orientation: inline

BBa_J64987
type: ComponentDefinition
persistent identity: BBa J64987
display id: BBa_J64987
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI Consensus Binding Site in sigma 70 binding region
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J64987_sequence/1

LacI
type: SequenceAnnotation
persistent identity: annotation1921610
display id: annotation1921610
version: 1
top level: BBa_J64987
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64987/annotation1921610/range1921610/1

https://synbiohub.org/public/igem/BBa_J64987/annotation1921610/range1921610/1
type: Range
persistent identity: range1921610
display id: range1921610
version: 1
direction: direction/forward
top level: BBa_J64987
start: 7
end: 26
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921608
display id: annotation1921608
version: 1
top level: BBa_J64987
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64987/annotation1921608/range1921608/1

https://synbiohub.org/public/igem/BBa_J64987/annotation1921608/range1921608/1
type: Range
persistent identity: range1921608
display id: range1921608
version: 1
direction: direction/forward
top level: BBa_J64987
start: 1
end: 6
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921609
display id: annotation1921609
version: 1
top level: BBa_J64987
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64987/annotation1921609/range1921609/1

https://synbiohub.org/public/igem/BBa_J64987/annotation1921609/range1921609/1
type: Range
persistent identity: range1921609
display id: range1921609
version: 1
direction: direction/forward
top level: BBa_J64987
start: 27
end: 32
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter P(Lac) IQ
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive Promoter, High Transcription
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 26
end: 31
orientation: inline

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 3
end: 8
orientation: inline

BBa_J64712
type: ComponentDefinition
persistent identity: BBa J64712
display id: BBa_J64712
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/LasI Inducible & RHLR/RHLI repressible Promoter
creator: laura lavery
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLI binding.
mutable notes: The natural spacer between the -35 and -10 is 14bp and the natural O1 site for RHLR/RHLI is 20bp long. There was not much over lap of the -35 or the -10 with this sequence, so it was nessicary to chop off some of the binding site sequence in order to maintain the correct spacing and preserve the -35 and -10 sequence. The binding site sequence is missing a tcc on is 5' end and a t on its 3' end.
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: RHLR/RHLI
sequence annotation: LasR/LasI O1
sequence annotation: -35
sequence annotation: LasR/LasI O2
sequence: https://synbiohub.org/public/igem/BBa_J64712_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1921068
display id: annotation1921068
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1
type: Range
persistent identity: range1921068
display id: range1921068
version: 1
direction: direction/forward
top level: BBa_J64712
start: 140
end: 145
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921066
display id: annotation1921066
version: 1
top level: BBa_J64712
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1
type: Range
persistent identity: range1921066
display id: range1921066
version: 1
direction: direction/forward
top level: BBa_J64712
start: 125
end: 141
orientation: inline

LasR/LasI O1
type: SequenceAnnotation
persistent identity: annotation1921065
display id: annotation1921065
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1
type: Range
persistent identity: range1921065
display id: range1921065
version: 1
direction: direction/forward
top level: BBa_J64712
start: 106
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921067
display id: annotation1921067
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1
type: Range
persistent identity: range1921067
display id: range1921067
version: 1
direction: direction/forward
top level: BBa_J64712
start: 117
end: 122
orientation: inline

LasR/LasI O2
type: SequenceAnnotation
persistent identity: annotation1921064
display id: annotation1921064
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1
type: Range
persistent identity: range1921064
display id: range1921064
version: 1
direction: direction/forward
top level: BBa_J64712
start: 46
end: 64
orientation: inline

BBa_I714889
type: ComponentDefinition
persistent identity: BBa I714889
display id: BBa_I714889
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OR21 of PR and PRM
creator: Chunbo Lou
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:07:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: we synthesize the OR promoter by replacing OR3 binding site of lambda CI with OR1 binding site of CI434.
mutable notes: making sure CI434 protein can repress CI's transcription.
mutable provenance: similar to lambda lysis/lysogenic regulatory region.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR1_434
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I714889_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1951425
display id: annotation1951425
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951425/range1951425/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951425/range1951425/1
type: Range
persistent identity: range1951425
display id: range1951425
version: 1
direction: direction/forward
top level: BBa_I714889
start: 67
end: 72
orientation: inline

OR1_434
type: SequenceAnnotation
persistent identity: annotation1951383
display id: annotation1951383
version: 1
top level: BBa_I714889
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951383/range1951383/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951383/range1951383/1
type: Range
persistent identity: range1951383
display id: range1951383
version: 1
direction: direction/forward
top level: BBa_I714889
start: 1
end: 14
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1951381
display id: annotation1951381
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951381/range1951381/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951381/range1951381/1
type: Range
persistent identity: range1951381
display id: range1951381
version: 1
direction: direction/forward
top level: BBa_I714889
start: 89
end: 95
orientation: inline

OR1_434
type: SequenceAnnotation
persistent identity: annotation1951380
display id: annotation1951380
version: 1
top level: BBa_I714889
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951380/range1951380/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951380/range1951380/1
type: Range
persistent identity: range1951380
display id: range1951380
version: 1
direction: direction/forward
top level: BBa_I714889
start: 31
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1951382
display id: annotation1951382
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951382/range1951382/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951382/range1951382/1
type: Range
persistent identity: range1951382
display id: range1951382
version: 1
direction: direction/reverse
top level: BBa_I714889
start: 49
end: 54
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1951424
display id: annotation1951424
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951424/range1951424/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951424/range1951424/1
type: Range
persistent identity: range1951424
display id: range1951424
version: 1
direction: direction/reverse
top level: BBa_I714889
start: 26
end: 31
orientation: inline

BBa_J07019
type: ComponentDefinition
persistent identity: BBa J07019
display id: BBa_J07019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA Promoter (with Fur box)
creator: jxyu
created: 2005-07-12T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: true
experience: experience/None
group u list: _21_
m user id: 0
owner id: 384
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: In native e.coli, this promoter is located upstream of FecA cds. Regulated by FecI protein, which is in turn regulated by FecA.
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Fur Box (binds Fur)
sequence annotation: -59 to -1 region
sequence annotation: FecI binding region
sequence: https://synbiohub.org/public/igem/BBa_J07019_sequence/1

Fur Box (binds Fur)
type: SequenceAnnotation
persistent identity: annotation1579238
display id: annotation1579238
version: 1
top level: BBa_J07019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J07019/annotation1579238/range1579238/1

https://synbiohub.org/public/igem/BBa_J07019/annotation1579238/range1579238/1
type: Range
persistent identity: range1579238
display id: range1579238
version: 1
direction: direction/forward
top level: BBa_J07019
start: 25
end: 39
orientation: inline

-59 to -1 region
type: SequenceAnnotation
persistent identity: annotation1579189
display id: annotation1579189
version: 1
top level: BBa_J07019
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07019/annotation1579189/range1579189/1

https://synbiohub.org/public/igem/BBa_J07019/annotation1579189/range1579189/1
type: Range
persistent identity: range1579189
display id: range1579189
version: 1
direction: direction/forward
top level: BBa_J07019
start: 1
end: 60
orientation: inline

FecI binding region
type: SequenceAnnotation
persistent identity: annotation1579239
display id: annotation1579239
version: 1
top level: BBa_J07019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J07019/annotation1579239/range1579239/1

https://synbiohub.org/public/igem/BBa_J07019/annotation1579239/range1579239/1
type: Range
persistent identity: range1579239
display id: range1579239
version: 1
direction: direction/forward
top level: BBa_J07019
start: 70
end: 76
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732430
display id: BBa_I732430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732430_sequence/1

BBa_K086017
type: ComponentDefinition
persistent identity: BBa K086017
display id: BBa_K086017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: unmodified Lutz-Bujard LacO promoter
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: unmodified Lutz-Bujard LacO promoter.
mutable notes: Cleaned
mutable provenance: Lac O
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacO
sequence: https://synbiohub.org/public/igem/BBa_K086017_sequence/1

LacO
type: SequenceAnnotation
persistent identity: annotation1995751
display id: annotation1995751
version: 1
top level: BBa_K086017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086017/annotation1995751/range1995751/1

https://synbiohub.org/public/igem/BBa_K086017/annotation1995751/range1995751/1
type: Range
persistent identity: range1995751
display id: range1995751
version: 1
direction: direction/forward
top level: BBa_K086017
start: 1
end: 55
orientation: inline

BBa_R0184
type: ComponentDefinition
persistent identity: BBa R0184
display id: BBa_R0184
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2007-04-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
mutable notes: The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0183 fragment
sequence annotation: Center of binding site symmetry
sequence annotation: lacI binding site
sequence: https://synbiohub.org/public/igem/BBa_R0184_sequence/1

BBa_R0183 fragment
type: SequenceAnnotation
persistent identity: annotation1932678
display id: annotation1932678
version: 1
top level: BBa_R0184
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
type: Range
persistent identity: range1932678
display id: range1932678
version: 1
direction: direction/forward
top level: BBa_R0184
start: 1
end: 20
orientation: inline

Center of binding site symmetry
type: SequenceAnnotation
persistent identity: annotation1932680
display id: annotation1932680
version: 1
top level: BBa_R0184
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
type: Range
persistent identity: range1932680
display id: range1932680
version: 1
direction: direction/forward
top level: BBa_R0184
start: 31
end: 31
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1932679
display id: annotation1932679
version: 1
top level: BBa_R0184
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
type: Range
persistent identity: range1932679
display id: range1932679
version: 1
direction: direction/forward
top level: BBa_R0184
start: 20
end: 44
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732437
display id: BBa_I732437
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732437_sequence/1

BBa_K598019
type: ComponentDefinition
persistent identity: BBa K598019
display id: BBa_K598019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE
creator: Zhenrun ZHANG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8606
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE
mutable notes: the Ribosomal Binding Site of vioE is contained in TPP Ribozyme.
mutable provenance: Hammerhead Ribozyme backbone comes from E.coli, and TPP aptamer comes from B.subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: vioB
sequence annotation: vioE
sequence annotation: B0015
sequence annotation: pBAD
sequence annotation: TPP ribozyme 2.5
sequence annotation: vioD
sequence annotation: rbs
sequence annotation: vioA
sequence: https://synbiohub.org/public/igem/BBa_K598019_sequence/1

vioB
type: SequenceAnnotation
persistent identity: annotation2144599
display id: annotation2144599
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144599/range2144599/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144599/range2144599/1
type: Range
persistent identity: range2144599
display id: range2144599
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1559
end: 4556
orientation: inline

vioE
type: SequenceAnnotation
persistent identity: annotation2144631
display id: annotation2144631
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144631/range2144631/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144631/range2144631/1
type: Range
persistent identity: range2144631
display id: range2144631
version: 1
direction: direction/forward
top level: BBa_K598019
start: 6135
end: 6710
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2144601
display id: annotation2144601
version: 1
top level: BBa_K598019
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144601/range2144601/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144601/range2144601/1
type: Range
persistent identity: range2144601
display id: range2144601
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5712
end: 5841
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2144595
display id: annotation2144595
version: 1
top level: BBa_K598019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144595/range2144595/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144595/range2144595/1
type: Range
persistent identity: range2144595
display id: range2144595
version: 1
direction: direction/forward
top level: BBa_K598019
start: 138
end: 267
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2144602
display id: annotation2144602
version: 1
top level: BBa_K598019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144602/range2144602/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144602/range2144602/1
type: Range
persistent identity: range2144602
display id: range2144602
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5849
end: 5978
orientation: inline

TPP ribozyme 2.5
type: SequenceAnnotation
persistent identity: annotation2144624
display id: annotation2144624
version: 1
top level: BBa_K598019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144624/range2144624/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144624/range2144624/1
type: Range
persistent identity: range2144624
display id: range2144624
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5985
end: 6134
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2144593
display id: annotation2144593
version: 1
top level: BBa_K598019
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144593/range2144593/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144593/range2144593/1
type: Range
persistent identity: range2144593
display id: range2144593
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1
end: 129
orientation: inline

vioD
type: SequenceAnnotation
persistent identity: annotation2144600
display id: annotation2144600
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144600/range2144600/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144600/range2144600/1
type: Range
persistent identity: range2144600
display id: range2144600
version: 1
direction: direction/forward
top level: BBa_K598019
start: 4579
end: 5704
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144630
display id: annotation2144630
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144630/range2144630/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144630/range2144630/1
type: Range
persistent identity: range2144630
display id: range2144630
version: 1
direction: direction/forward
top level: BBa_K598019
start: 6122
end: 6129
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144598
display id: annotation2144598
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144598/range2144598/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144598/range2144598/1
type: Range
persistent identity: range2144598
display id: range2144598
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1545
end: 1551
orientation: inline

vioA
type: SequenceAnnotation
persistent identity: annotation2144597
display id: annotation2144597
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144597/range2144597/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144597/range2144597/1
type: Range
persistent identity: range2144597
display id: range2144597
version: 1
direction: direction/forward
top level: BBa_K598019
start: 285
end: 1542
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144596
display id: annotation2144596
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144596/range2144596/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144596/range2144596/1
type: Range
persistent identity: range2144596
display id: range2144596
version: 1
direction: direction/forward
top level: BBa_K598019
start: 273
end: 279
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144594
display id: annotation2144594
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144594/range2144594/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144594/range2144594/1
type: Range
persistent identity: range2144594
display id: range2144594
version: 1
direction: direction/forward
top level: BBa_K598019
start: 4565
end: 4571
orientation: inline

BBa_K819002
type: ComponentDefinition
persistent identity: BBa K819002
display id: BBa_K819002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luminesensor repressible recA408 promoter
creator: YU Zhou
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: recA promoter in 408 form.recA promoter belongs to the SOS regulon family, and promotes in E.coli transcription of recA protein, which forms filament when bound to ssDNA and trigger the self-cleavage of LexA protein.recA408 have one SOS box, which binds to the dimerized LexA protein and cause inhibition of the transcription of downstream gene.
mutable notes: The original RecA was mutated to 408 form in order to be responsive to 408 mutant form of LexA DNA binding domain.
mutable provenance: This comes later.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: transcription start site
sequence annotation: RecA 408 promoter
sequence: https://synbiohub.org/public/igem/BBa_K819002_sequence/1

transcription start site
type: SequenceAnnotation
persistent identity: annotation2181610
display id: annotation2181610
version: 1
top level: BBa_K819002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K819002/annotation2181610/range2181610/1

https://synbiohub.org/public/igem/BBa_K819002/annotation2181610/range2181610/1
type: Range
persistent identity: range2181610
display id: range2181610
version: 1
direction: direction/forward
top level: BBa_K819002
start: 61
end: 61
orientation: inline

RecA 408 promoter
type: SequenceAnnotation
persistent identity: annotation2181609
display id: annotation2181609
version: 1
top level: BBa_K819002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819002/annotation2181609/range2181609/1

https://synbiohub.org/public/igem/BBa_K819002/annotation2181609/range2181609/1
type: Range
persistent identity: range2181609
display id: range2181609
version: 1
direction: direction/forward
top level: BBa_K819002
start: 1
end: 81
orientation: inline

BBa_K1861040
type: ComponentDefinition
persistent identity: BBa K1861040
display id: BBa_K1861040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SpyCatcher and RPD3 in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:21:28Z
discontinued: false
dominant: false
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1861401
component: BBa_K1861101
component: BBa_K1159200
component: BBa_K1861104
component: BBa_K1861102
component: BBa_K392003
sequence annotation: BBa_K1861401
sequence annotation: BBa_K1861102
sequence annotation: BBa_K1861104
sequence annotation: BBa_K392003
sequence annotation: BBa_K1159200
sequence annotation: BBa_K1861101
sequence: https://synbiohub.org/public/igem/BBa_K1861040_sequence/1

BBa_K1861401
type: Component
persistent identity: component2474407
display id: component2474407
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861401/1

BBa_K1861101
type: Component
persistent identity: component2474400
display id: component2474400
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1159200
type: Component
persistent identity: component2474406
display id: component2474406
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159200/1

BBa_K1861104
type: Component
persistent identity: component2474408
display id: component2474408
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1861102
type: Component
persistent identity: component2474401
display id: component2474401
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K392003
type: Component
persistent identity: component2474409
display id: component2474409
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K1861401
type: SequenceAnnotation
persistent identity: annotation2474407
display id: annotation2474407
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474407/range2474407/1
component: BBa_K1861401

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474407/range2474407/1
type: Range
persistent identity: range2474407
display id: range2474407
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 605
end: 1954
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2474401
display id: annotation2474401
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474401/range2474401/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474401/range2474401/1
type: Range
persistent identity: range2474401
display id: range2474401
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 239
end: 265
orientation: inline

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2474408
display id: annotation2474408
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474408/range2474408/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474408/range2474408/1
type: Range
persistent identity: range2474408
display id: range2474408
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1955
end: 1963
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2474409
display id: annotation2474409
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474409/range2474409/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474409/range2474409/1
type: Range
persistent identity: range2474409
display id: range2474409
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1964
end: 2092
orientation: inline

BBa_K1159200
type: SequenceAnnotation
persistent identity: annotation2474406
display id: annotation2474406
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474406/range2474406/1
component: BBa_K1159200

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474406/range2474406/1
type: Range
persistent identity: range2474406
display id: range2474406
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 266
end: 604
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2474400
display id: annotation2474400
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474400/range2474400/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474400/range2474400/1
type: Range
persistent identity: range2474400
display id: range2474400
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1
end: 238
orientation: inline

Cpn60/10
type: ComponentDefinition
persistent identity: BBa K538211
display id: BBa_K538211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cpn60/10 operon (pLacI)
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K538000
component: BBa_K538001
component: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_K538001
sequence annotation: BBa_K538000
sequence: https://synbiohub.org/public/igem/BBa_K538211_sequence/1

BBa_B0034
type: Component
persistent identity: component2222782
display id: component2222782
version: 1
top level: Cpn60/10
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538000
type: Component
persistent identity: component2222791
display id: component2222791
version: 1
top level: Cpn60/10
access: public
definition: https://synbiohub.org/public/igem/BBa_K538000/1

BBa_K538001
type: Component
persistent identity: component2222786
display id: component2222786
version: 1
top level: Cpn60/10
access: public
definition: https://synbiohub.org/public/igem/BBa_K538001/1

BBa_B0034
type: Component
persistent identity: component2222788
display id: component2222788
version: 1
top level: Cpn60/10
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component2222774
display id: component2222774
version: 1
top level: Cpn60/10
access: public
definition: LacI

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2222788
display id: annotation2222788
version: 1
top level: Cpn60/10
location: https://synbiohub.org/public/igem/BBa_K538211/annotation2222788/range2222788/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538211/annotation2222788/range2222788/1
type: Range
persistent identity: range2222788
display id: range2222788
version: 1
direction: direction/reverse
top level: Cpn60/10
start: 1885
end: 1896
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2222774
display id: annotation2222774
version: 1
top level: Cpn60/10
location: https://synbiohub.org/public/igem/BBa_K538211/annotation2222774/range2222774/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538211/annotation2222774/range2222774/1
type: Range
persistent identity: range2222774
display id: range2222774
version: 1
direction: direction/forward
top level: Cpn60/10
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2222782
display id: annotation2222782
version: 1
top level: Cpn60/10
location: https://synbiohub.org/public/igem/BBa_K538211/annotation2222782/range2222782/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538211/annotation2222782/range2222782/1
type: Range
persistent identity: range2222782
display id: range2222782
version: 1
direction: direction/reverse
top level: Cpn60/10
start: 209
end: 220
orientation: inline

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2222786
display id: annotation2222786
version: 1
top level: Cpn60/10
location: https://synbiohub.org/public/igem/BBa_K538211/annotation2222786/range2222786/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538211/annotation2222786/range2222786/1
type: Range
persistent identity: range2222786
display id: range2222786
version: 1
direction: direction/reverse
top level: Cpn60/10
start: 227
end: 1876
orientation: inline

BBa_K538000
type: SequenceAnnotation
persistent identity: annotation2222791
display id: annotation2222791
version: 1
top level: Cpn60/10
location: https://synbiohub.org/public/igem/BBa_K538211/annotation2222791/range2222791/1
component: BBa_K538000

https://synbiohub.org/public/igem/BBa_K538211/annotation2222791/range2222791/1
type: Range
persistent identity: range2222791
display id: range2222791
version: 1
direction: direction/reverse
top level: Cpn60/10
start: 1903
end: 2199
orientation: inline

NAND2616
type: ComponentDefinition
persistent identity: BBa I732301
display id: BBa_I732301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O26D001O16)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O11
mutable notes: N/A
mutable provenance: LacIq
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732301_sequence/1

BBa_K109200
type: ComponentDefinition
persistent identity: BBa K109200
display id: BBa_K109200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC and TetR promoter (hybrid)
creator: Faifan Tantakitti
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:09:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _250_
m user id: 0
owner id: 3345
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is lacI. trial version.
mutable notes: ......blank2....
mutable provenance: .....blank1...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR
sequence annotation: -10
sequence annotation: -35
sequence annotation: AraC
sequence: https://synbiohub.org/public/igem/BBa_K109200_sequence/1

TetR
type: SequenceAnnotation
persistent identity: annotation1969066
display id: annotation1969066
version: 1
top level: BBa_K109200
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1969066/range1969066/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1969066/range1969066/1
type: Range
persistent identity: range1969066
display id: range1969066
version: 1
direction: direction/forward
top level: BBa_K109200
start: 81
end: 99
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1980471
display id: annotation1980471
version: 1
top level: BBa_K109200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1980471/range1980471/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1980471/range1980471/1
type: Range
persistent identity: range1980471
display id: range1980471
version: 1
direction: direction/forward
top level: BBa_K109200
start: 73
end: 78
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1980470
display id: annotation1980470
version: 1
top level: BBa_K109200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1980470/range1980470/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1980470/range1980470/1
type: Range
persistent identity: range1980470
display id: range1980470
version: 1
direction: direction/forward
top level: BBa_K109200
start: 49
end: 54
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation1969061
display id: annotation1969061
version: 1
top level: BBa_K109200
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1969061/range1969061/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1969061/range1969061/1
type: Range
persistent identity: range1969061
display id: range1969061
version: 1
direction: direction/forward
top level: BBa_K109200
start: 34
end: 50
orientation: inline

BBa_K086027
type: ComponentDefinition
persistent identity: BBa K086027
display id: BBa_K086027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_2
sequence: https://synbiohub.org/public/igem/BBa_K086027_sequence/1

P32_2
type: SequenceAnnotation
persistent identity: annotation1995761
display id: annotation1995761
version: 1
top level: BBa_K086027
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086027/annotation1995761/range1995761/1

https://synbiohub.org/public/igem/BBa_K086027/annotation1995761/range1995761/1
type: Range
persistent identity: range1995761
display id: range1995761
version: 1
direction: direction/forward
top level: BBa_K086027
start: 1
end: 55
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732405
display id: BBa_I732405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732402.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: stem_loop
sequence annotation: T7 TE
sequence annotation: BBa_I732006
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: barcode
sequence annotation: conserved
sequence annotation: stop
sequence annotation: BglII
sequence annotation: polya
sequence annotation: STOP
sequence annotation: mrfp1
sequence annotation: BBa_I732007
sequence annotation: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: lacZ
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_I732405_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1944405
display id: annotation1944405
version: 1
top level: U049O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944405/range1944405/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944405/range1944405/1
type: Range
persistent identity: range1944405
display id: range1944405
version: 1
direction: direction/forward
top level: U049O11+D0
start: 12
end: 17
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944419
display id: annotation1944419
version: 1
top level: U049O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944419/range1944419/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944419/range1944419/1
type: Range
persistent identity: range1944419
display id: range1944419
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1031
end: 1074
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944421
display id: annotation1944421
version: 1
top level: U049O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944421/range1944421/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944421/range1944421/1
type: Range
persistent identity: range1944421
display id: range1944421
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1115
end: 1134
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944412
display id: annotation1944412
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944412/range1944412/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944412/range1944412/1
type: Range
persistent identity: range1944412
display id: range1944412
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944408
display id: annotation1944408
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944408/range1944408/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944408/range1944408/1
type: Range
persistent identity: range1944408
display id: range1944408
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 28
end: 39
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944418
display id: annotation1944418
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944418/range1944418/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944418/range1944418/1
type: Range
persistent identity: range1944418
display id: range1944418
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1020
end: 1099
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1944417
display id: annotation1944417
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944417/range1944417/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944417/range1944417/1
type: Range
persistent identity: range1944417
display id: range1944417
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 987
end: 1011
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944407
display id: annotation1944407
version: 1
top level: U049O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944407/range1944407/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944407/range1944407/1
type: Range
persistent identity: range1944407
display id: range1944407
version: 1
direction: direction/forward
top level: U049O11+D0
start: 32
end: 35
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944423
display id: annotation1944423
version: 1
top level: U049O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944423/range1944423/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944423/range1944423/1
type: Range
persistent identity: range1944423
display id: range1944423
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1141
end: 1141
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944404
display id: annotation1944404
version: 1
top level: U049O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944404/range1944404/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944404/range1944404/1
type: Range
persistent identity: range1944404
display id: range1944404
version: 1
direction: direction/forward
top level: U049O11+D0
start: 3
end: 8
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944422
display id: annotation1944422
version: 1
top level: U049O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944422/range1944422/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944422/range1944422/1
type: Range
persistent identity: range1944422
display id: range1944422
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1135
end: 1148
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944411
display id: annotation1944411
version: 1
top level: U049O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944411/range1944411/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944411/range1944411/1
type: Range
persistent identity: range1944411
display id: range1944411
version: 1
direction: direction/forward
top level: U049O11+D0
start: 274
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944414
display id: annotation1944414
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944414/range1944414/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944414/range1944414/1
type: Range
persistent identity: range1944414
display id: range1944414
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 288
end: 299
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1944415
display id: annotation1944415
version: 1
top level: U049O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944415/range1944415/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944415/range1944415/1
type: Range
persistent identity: range1944415
display id: range1944415
version: 1
direction: direction/forward
top level: U049O11+D0
start: 306
end: 980
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944406
display id: annotation1944406
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944406/range1944406/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944406/range1944406/1
type: Range
persistent identity: range1944406
display id: range1944406
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 1
end: 19
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1944416
display id: annotation1944416
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944416/range1944416/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944416/range1944416/1
type: Range
persistent identity: range1944416
display id: range1944416
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 306
end: 986
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944413
display id: annotation1944413
version: 1
top level: U049O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944413/range1944413/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944413/range1944413/1
type: Range
persistent identity: range1944413
display id: range1944413
version: 1
direction: direction/forward
top level: U049O11+D0
start: 292
end: 295
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944420
display id: annotation1944420
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944420/range1944420/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944420/range1944420/1
type: Range
persistent identity: range1944420
display id: range1944420
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1108
end: 1148
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944410
display id: annotation1944410
version: 1
top level: U049O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944410/range1944410/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944410/range1944410/1
type: Range
persistent identity: range1944410
display id: range1944410
version: 1
direction: direction/forward
top level: U049O11+D0
start: 46
end: 279
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944409
display id: annotation1944409
version: 1
top level: U049O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944409/range1944409/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944409/range1944409/1
type: Range
persistent identity: range1944409
display id: range1944409
version: 1
direction: direction/forward
top level: U049O11+D0
start: 46
end: 48
orientation: inline

NANDbeta
type: ComponentDefinition
persistent identity: BBa I732306
display id: BBa_I732306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O11D002O22)
creator: Zhan Jian
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {| border="1" | |align="center"| |align="center"| |}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732306_sequence/1

Pspac-hy
type: ComponentDefinition
persistent identity: BBa I746665
display id: BBa_I746665
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspac-hy promoter
creator: Yue Miao
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2119
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: There is a G->T point mutation of pSpac at the -1 site, changing the promoter to a stronger one - hence the name pSpac-hy, hy meaning hyper. This promoter drives transcription at rates of about ten times that of the wild type (pSpac) promoter.
mutable notes: NA
mutable provenance: Comes from the pPL82 vector, which is intended as a B. subtilis integrational vector. It is placed in front of the MCS in the plasmid, as it would drive the expression of the gene cloned into the MCS both in B. subtilis and E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I746665_sequence/1

BBa_K259007
type: ComponentDefinition
persistent identity: BBa K259007
display id: BBa_K259007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Promoter fused with RBS
creator: Petros Mina
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has the arabinose regulated promoter (AraC/pBAD) upstream of a ribosome binding site. This is ideal for inserting a protein to be expressed downstream of this part as it's expression can be regulated by the presence of the concentration of arabinose in the growing environment/media of the cells. ==What this part actually does== This composite part includes the AraC/pBAD promoter and a strong ribosome binding site designed from the Anderson family of RBS. The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the trancription of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. Controlling the transcription will not ensure translation. The ribosome binding site is ideally placed a small distance away from the promoter site to ensure that the ribosome will bind and start translating the protein coding sequence found downstream.
mutable notes: The part was designed with RFC10 assembly from supplied biobricks of the iGEM2009 distribution.
mutable provenance: The AraC promoter can be found naturally at 1.52min of the E.coli genome ([http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene number EG10054]. The RBS was taken from the designed sequences of RBS by the Anderson family. For more information visit the original part page [http://partsregistry.org/Part:BBa_J61100 here].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J61100
component: BBa_R0081
component: BBa_R0080
sequence annotation: BBa_R0081
sequence annotation: BBa_R0080
sequence annotation: BBa_J61100
sequence: https://synbiohub.org/public/igem/BBa_K259007_sequence/1

BBa_J61100
type: Component
persistent identity: component2020547
display id: component2020547
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_R0081
type: Component
persistent identity: component2020539
display id: component2020539
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2020546
display id: component2020546
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2020539
display id: annotation2020539
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
type: Range
persistent identity: range2020539
display id: range2020539
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 1
end: 183
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2020546
display id: annotation2020546
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
type: Range
persistent identity: range2020546
display id: range2020546
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 192
end: 340
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2020547
display id: annotation2020547
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
type: Range
persistent identity: range2020547
display id: range2020547
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 349
end: 360
orientation: inline

BBa_K112402
type: ComponentDefinition
persistent identity: BBa K112402
display id: BBa_K112402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter for FabA gene - Membrane Damage and Ultrasound Senstitive
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the fabA gene, a known membrane damage response gene, is upregulated seven fold when cells are exposed to high frequency ultrasound and microbubble cavitation.
mutable notes: none
mutable provenance: cloned from E. coli MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112402_sequence/1

BBa_S04209
type: ComponentDefinition
persistent identity: BBa S04209
display id: BBa_S04209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0051:Q04121:B0034:C0079:B0015
creator: UNAM FCiencias-CINVESTAV IRA
created: 2009-06-02T11:00:00Z
modified: 2015-05-08T01:14:36Z
discontinued: false
dominant: true
experience: experience/None
group u list: _9_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S04208
component: BBa_B0034
sequence annotation: BBa_S04208
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04209_sequence/1

BBa_S04208
type: Component
persistent identity: component2267293
display id: component2267293
version: 1
top level: BBa_S04209
access: public
definition: https://synbiohub.org/public/igem/BBa_S04208/1

BBa_B0034
type: Component
persistent identity: component2267295
display id: component2267295
version: 1
top level: BBa_S04209
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_S04208
type: SequenceAnnotation
persistent identity: annotation2267293
display id: annotation2267293
version: 1
top level: BBa_S04209
location: https://synbiohub.org/public/igem/BBa_S04209/annotation2267293/range2267293/1
component: BBa_S04208

https://synbiohub.org/public/igem/BBa_S04209/annotation2267293/range2267293/1
type: Range
persistent identity: range2267293
display id: range2267293
version: 1
direction: direction/reverse
top level: BBa_S04209
start: 1
end: 1414
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267295
display id: annotation2267295
version: 1
top level: BBa_S04209
location: https://synbiohub.org/public/igem/BBa_S04209/annotation2267295/range2267295/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04209/annotation2267295/range2267295/1
type: Range
persistent identity: range2267295
display id: range2267295
version: 1
direction: direction/reverse
top level: BBa_S04209
start: 1423
end: 1434
orientation: inline

BBa_K174004
type: ComponentDefinition
persistent identity: BBa K174004
display id: BBa_K174004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspac promoter
creator: The Newcastle 2009 iGEM team
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:10:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _277_
m user id: 0
owner id: 3942
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter can be induced by IPTG. Wild type LacI binding site is replaced with a perfect palindromic "oid" operator sequence which increases the repression by LacI. This biobrick is an enhancement of Biobrick BBa_K090501 which has the sequence from pmutin2.
mutable notes: pmutin2's genbank accession number is AF072806. Although there is no sequence information for pmutin4, it can be derived from pmutin2's sequence information by changing the O1 lacI binding site with the "oid" sequence.
mutable provenance: This sequence is taken from pmutin4 integration vector. pmutin2's lacI binding site was changed with the "oid" sequence to create pmutin4.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: oid - Lac operator
sequence annotation: pspac promoter
sequence: https://synbiohub.org/public/igem/BBa_K174004_sequence/1

oid - Lac operator
type: SequenceAnnotation
persistent identity: annotation2033093
display id: annotation2033093
version: 1
top level: BBa_K174004
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K174004/annotation2033093/range2033093/1

https://synbiohub.org/public/igem/BBa_K174004/annotation2033093/range2033093/1
type: Range
persistent identity: range2033093
display id: range2033093
version: 1
direction: direction/forward
top level: BBa_K174004
start: 81
end: 100
orientation: inline

pspac promoter
type: SequenceAnnotation
persistent identity: annotation2033094
display id: annotation2033094
version: 1
top level: BBa_K174004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K174004/annotation2033094/range2033094/1

https://synbiohub.org/public/igem/BBa_K174004/annotation2033094/range2033094/1
type: Range
persistent identity: range2033094
display id: range2033094
version: 1
direction: direction/forward
top level: BBa_K174004
start: 43
end: 80
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressible promoter
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR 2
sequence annotation: -10
sequence annotation: -35
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
direction: direction/forward
top level: p(tetR)
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
direction: direction/forward
top level: p(tetR)
start: 43
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
direction: direction/forward
top level: p(tetR)
start: 20
end: 25
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 19
orientation: inline

BBa_I739106
type: ComponentDefinition
persistent identity: BBa I739106
display id: BBa_I739106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (TetR, negative / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-15T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R0053 (2x OR1)
sequence annotation: OR1
sequence annotation: R0040
sequence: https://synbiohub.org/public/igem/BBa_I739106_sequence/1

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947941
display id: annotation1947941
version: 1
top level: BBa_I739106
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947941/range1947941/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947941/range1947941/1
type: Range
persistent identity: range1947941
display id: range1947941
version: 1
direction: direction/forward
top level: BBa_I739106
start: 49
end: 84
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947939
display id: annotation1947939
version: 1
top level: BBa_I739106
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947939/range1947939/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947939/range1947939/1
type: Range
persistent identity: range1947939
display id: range1947939
version: 1
direction: direction/forward
top level: BBa_I739106
start: 49
end: 66
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947940
display id: annotation1947940
version: 1
top level: BBa_I739106
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947940/range1947940/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947940/range1947940/1
type: Range
persistent identity: range1947940
display id: range1947940
version: 1
direction: direction/forward
top level: BBa_I739106
start: 67
end: 84
orientation: inline

R0040
type: SequenceAnnotation
persistent identity: annotation1947938
display id: annotation1947938
version: 1
top level: BBa_I739106
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947938/range1947938/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947938/range1947938/1
type: Range
persistent identity: range1947938
display id: range1947938
version: 1
direction: direction/forward
top level: BBa_I739106
start: 1
end: 48
orientation: inline

BBa_J34809
type: ComponentDefinition
persistent identity: BBa J34809
display id: BBa_J34809
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter lac induced
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: improved for transcription factor BBa_J34807 binding
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34809_sequence/1

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lambda cI and luxR regulated -- hybrid)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight AF170104 .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2 cI
sequence annotation: -10
sequence annotation: -35
sequence annotation: OR1 cI
sequence annotation: lux p(R) start
sequence annotation: BBa_R0065
sequence annotation: Lux Box
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
direction: direction/forward
top level: cI+luxR
start: 57
end: 73
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
direction: direction/forward
top level: cI+luxR
start: 94
end: 97
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
direction: direction/forward
top level: cI+luxR
start: 71
end: 76
orientation: inline

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
direction: direction/forward
top level: cI+luxR
start: 81
end: 97
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
direction: direction/forward
top level: cI+luxR
start: 58
end: 58
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
direction: direction/forward
top level: cI+luxR
start: 1
end: 97
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
direction: direction/forward
top level: cI+luxR
start: 6
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
direction: direction/forward
top level: cI+luxR
start: 47
end: 52
orientation: inline

BBa_K136004
type: ComponentDefinition
persistent identity: BBa K136004
display id: BBa_K136004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flhDC gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''flhDC'' is the only class 1 gene that is part of the construction of the flagella. It is the master regulator of the biosynthesis of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). The promoter of ''flhDC'' is regulated by many transcription factors, for instance, OmpR* and EnvZ* are two repressors of this gene. '''Work in progress :''' Characterization of the repression by OmpR* and EnvZ*
mutable notes: The PCR on colony to amplify the promoter of ''flhDC'' did not work. We amplified both the promoter and the gene and then we did a specific amplification of the promoter from the full operon. We had a PCR amplicon of the right length this time. The primers used to amplify the promoter and the gene are : * Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG * Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAACAGCCTGTACTCTCTGTTCATCC Then to amplify only the promoter we used : * Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG * Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OmpR binding site
sequence annotation: Transcription start base
sequence: https://synbiohub.org/public/igem/BBa_K136004_sequence/1

OmpR binding site
type: SequenceAnnotation
persistent identity: annotation1971578
display id: annotation1971578
version: 1
top level: BBa_K136004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136004/annotation1971578/range1971578/1

https://synbiohub.org/public/igem/BBa_K136004/annotation1971578/range1971578/1
type: Range
persistent identity: range1971578
display id: range1971578
version: 1
direction: direction/forward
top level: BBa_K136004
start: 43
end: 63
orientation: inline

Transcription start base
type: SequenceAnnotation
persistent identity: annotation1971577
display id: annotation1971577
version: 1
top level: BBa_K136004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136004/annotation1971577/range1971577/1

https://synbiohub.org/public/igem/BBa_K136004/annotation1971577/range1971577/1
type: Range
persistent identity: range1971577
display id: range1971577
version: 1
direction: direction/forward
top level: BBa_K136004
start: 36
end: 36
orientation: inline

BBa_K131001
type: ComponentDefinition
persistent identity: BBa K131001
display id: BBa_K131001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ColE2 SOS Promoter
creator: Kevin McLeod
created: 2008-07-21T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: true
dominant: false
experience: experience/None
group u list: _259_
m user id: 0
owner id: 1917
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This promoter normally represses the ColE2 activity gene, ceaB, in the ColE2 operon. To activate this promoter would require a RecA positive strain, as the protein LexA represses the promoter. To induce this promoter, an intercalating agent, mitomycin C, was utilized to induce the SOS response, and thus the expression of the genes under control of this promoter.
mutable notes: Lab strains of E. coli, such as Top10 from Invitrogen, are deficient in RecA so we required a RecA positive strain to induce this promoter. We used BL21 from Novagen.
mutable provenance: This part comes from the ColE2 operon, which came from a plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: SOS Box
sequence annotation: Region of Dyad Symmetry
sequence annotation: -35 Signal
sequence annotation: -15 Signal
sequence: https://synbiohub.org/public/igem/BBa_K131001_sequence/1

SOS Box
type: SequenceAnnotation
persistent identity: annotation1968167
display id: annotation1968167
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968167/range1968167/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968167/range1968167/1
type: Range
persistent identity: range1968167
display id: range1968167
version: 1
direction: direction/forward
top level: BBa_K131001
start: 252
end: 267
orientation: inline

Region of Dyad Symmetry
type: SequenceAnnotation
persistent identity: annotation1968166
display id: annotation1968166
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968166/range1968166/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968166/range1968166/1
type: Range
persistent identity: range1968166
display id: range1968166
version: 1
direction: direction/forward
top level: BBa_K131001
start: 136
end: 161
orientation: inline

-35 Signal
type: SequenceAnnotation
persistent identity: annotation1968164
display id: annotation1968164
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968164/range1968164/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968164/range1968164/1
type: Range
persistent identity: range1968164
display id: range1968164
version: 1
direction: direction/forward
top level: BBa_K131001
start: 218
end: 224
orientation: inline

-15 Signal
type: SequenceAnnotation
persistent identity: annotation1968165
display id: annotation1968165
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968165/range1968165/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968165/range1968165/1
type: Range
persistent identity: range1968165
display id: range1968165
version: 1
direction: direction/forward
top level: BBa_K131001
start: 241
end: 246
orientation: inline

BBa_K086024
type: ComponentDefinition
persistent identity: BBa K086024
display id: BBa_K086024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: cleaned
mutable provenance: Lac O and sigma 28 binding sites' conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_3
sequence: https://synbiohub.org/public/igem/BBa_K086024_sequence/1

P24_3
type: SequenceAnnotation
persistent identity: annotation1995758
display id: annotation1995758
version: 1
top level: BBa_K086024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086024/annotation1995758/range1995758/1

https://synbiohub.org/public/igem/BBa_K086024/annotation1995758/range1995758/1
type: Range
persistent identity: range1995758
display id: range1995758
version: 1
direction: direction/forward
top level: BBa_K086024
start: 1
end: 55
orientation: inline

BBa_K119002
type: ComponentDefinition
persistent identity: BBa K119002
display id: BBa_K119002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RcnR operator (represses RcnA)
creator: Libertad Pantoja
created: 2008-09-27T11:00:00Z
modified: 2015-05-08T01:09:38Z
discontinued: false
dominant: false
group u list: _235_
m user id: 0
owner id: 3054
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Operator for RcnR(YohL) the negative regulator of niquel extrusion pump(RcnA) in E.coli
mutable notes: We needed the RcnR operator without its promoter, so it was necessary to predict the promoter and discard it from the sequence.
mutable provenance: E.coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RcnRop
sequence: https://synbiohub.org/public/igem/BBa_K119002_sequence/1

RcnRop
type: SequenceAnnotation
persistent identity: annotation1977507
display id: annotation1977507
version: 1
top level: BBa_K119002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K119002/annotation1977507/range1977507/1

https://synbiohub.org/public/igem/BBa_K119002/annotation1977507/range1977507/1
type: Range
persistent identity: range1977507
display id: range1977507
version: 1
direction: direction/forward
top level: BBa_K119002
start: 1
end: 83
orientation: inline

BBa_K1468004
type: ComponentDefinition
persistent identity: BBa K1468004
display id: BBa_K1468004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + gene encoding LacZ
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: LacZ
sequence annotation: BBa_I732005
sequence annotation: BBa_K1468004
sequence annotation: LacZ protein
sequence annotation: -10
sequence annotation: TetR 2
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: STOP
sequence annotation: BBa_R0040
sequence annotation: C --> T
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_K1468004_sequence/1

LacZ
type: SequenceAnnotation
persistent identity: annotation2400596
display id: annotation2400596
version: 1
top level: BBa_K1468004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1
type: Range
persistent identity: range2400596
display id: range2400596
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2400600
display id: annotation2400600
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1
type: Range
persistent identity: range2400600
display id: range2400600
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

BBa_K1468004
type: SequenceAnnotation
persistent identity: annotation2400601
display id: annotation2400601
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1
type: Range
persistent identity: range2400601
display id: range2400601
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 3129
orientation: inline

LacZ protein
type: SequenceAnnotation
persistent identity: annotation2418063
display id: annotation2418063
version: 1
top level: BBa_K1468004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1
type: Range
persistent identity: range2418063
display id: range2418063
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2400595
display id: annotation2400595
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1
type: Range
persistent identity: range2400595
display id: range2400595
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 43
end: 48
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2400594
display id: annotation2400594
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1
type: Range
persistent identity: range2400594
display id: range2400594
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 26
end: 44
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2400591
display id: annotation2400591
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1
type: Range
persistent identity: range2400591
display id: range2400591
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2400592
display id: annotation2400592
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1
type: Range
persistent identity: range2400592
display id: range2400592
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 20
end: 25
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2400599
display id: annotation2400599
version: 1
top level: BBa_K1468004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1
type: Range
persistent identity: range2400599
display id: range2400599
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3129
end: 3129
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2400593
display id: annotation2400593
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1
type: Range
persistent identity: range2400593
display id: range2400593
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 54
orientation: inline

C --> T
type: SequenceAnnotation
persistent identity: annotation2400598
display id: annotation2400598
version: 1
top level: BBa_K1468004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1
type: Range
persistent identity: range2400598
display id: range2400598
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3085
end: 3085
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2400597
display id: annotation2400597
version: 1
top level: BBa_K1468004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1
type: Range
persistent identity: range2400597
display id: range2400597
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 63
orientation: inline

LacI:Cpn60
type: ComponentDefinition
persistent identity: BBa K538201
display id: BBa_K538201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + Cpn60
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K538001
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_K538001
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K538201_sequence/1

BBa_K538001
type: Component
persistent identity: component2219877
display id: component2219877
version: 1
top level: LacI:Cpn60
access: public
definition: https://synbiohub.org/public/igem/BBa_K538001/1

BBa_B0034
type: Component
persistent identity: component2219873
display id: component2219873
version: 1
top level: LacI:Cpn60
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component2219865
display id: component2219865
version: 1
top level: LacI:Cpn60
access: public
definition: LacI

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2219877
display id: annotation2219877
version: 1
top level: LacI:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538201/annotation2219877/range2219877/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538201/annotation2219877/range2219877/1
type: Range
persistent identity: range2219877
display id: range2219877
version: 1
direction: direction/reverse
top level: LacI:Cpn60
start: 227
end: 1876
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219873
display id: annotation2219873
version: 1
top level: LacI:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538201/annotation2219873/range2219873/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538201/annotation2219873/range2219873/1
type: Range
persistent identity: range2219873
display id: range2219873
version: 1
direction: direction/reverse
top level: LacI:Cpn60
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2219865
display id: annotation2219865
version: 1
top level: LacI:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538201/annotation2219865/range2219865/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538201/annotation2219865/range2219865/1
type: Range
persistent identity: range2219865
display id: range2219865
version: 1
direction: direction/forward
top level: LacI:Cpn60
start: 1
end: 200
orientation: inline

BBa_J56012
type: ComponentDefinition
persistent identity: BBa J56012
display id: BBa_J56012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Invertible sequence of dna includes Ptrc promoter
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Seuqence is inverted by the FimE protein. Inside recognition sequence is a Ptrc promoter sequence. Switch is irreversible.
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J56012_sequence/1

NAND2-7
type: ComponentDefinition
persistent identity: BBa I732304
display id: BBa_I732304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D001O47)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O44
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732304_sequence/1

BBa_I756014
type: ComponentDefinition
persistent identity: BBa I756014
display id: BBa_I756014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexAoperator-MajorLatePromoter
creator: Johannes Hugo Decker
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2071
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains the LexAoperator and MajorLatePromoter genes with restriction site KdeI at the 5 prime end and AscI at the 3' end. Will use it as both a regulatory part for certain signals as well as a promoter.
mutable notes: Had to make sure the restriction sites used were not in the sequence or in the sequence of any of the genes downstream of the promoter.
mutable provenance: genbank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756014_sequence/1

BBa_S03385
type: ComponentDefinition
persistent identity: BBa S03385
display id: BBa_S03385
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cold-sensing promoter (hybB)
creator: Elizabeth Clarke
created: 2005-11-03T12:00:00Z
modified: 2015-05-08T01:14:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _9_
m user id: 0
owner id: 473
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: This is the hybB promoter, which normally controlls hydrogenase regulation at low temperatures. It turns ON at 30oC and is active as low as 20oC (lower not tested). It is OFF at 37 and higher temperatures. In the literature, it is also active in anaerobic environments. This hasn't been tested.
mutable provenance: lizjaneclarke@gmail.com Univerity of California - San Francisco
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory

BBa_K086028
type: ComponentDefinition
persistent identity: BBa K086028
display id: BBa_K086028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites' promoter sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_3
sequence: https://synbiohub.org/public/igem/BBa_K086028_sequence/1

P32_3
type: SequenceAnnotation
persistent identity: annotation1995762
display id: annotation1995762
version: 1
top level: BBa_K086028
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086028/annotation1995762/range1995762/1

https://synbiohub.org/public/igem/BBa_K086028/annotation1995762/range1995762/1
type: Range
persistent identity: range1995762
display id: range1995762
version: 1
direction: direction/forward
top level: BBa_K086028
start: 1
end: 55
orientation: inline

BBa_I756019
type: ComponentDefinition
persistent identity: BBa I756019
display id: BBa_I756019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Operator in SV-40 intron
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: true
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Lac Operator in SV-40 intron
mutable notes: Lac Operator in SV-40 intron
mutable provenance: Lac Operator in SV-40 intron
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756019_sequence/1

BBa_K266001
type: ComponentDefinition
persistent identity: BBa K266001
display id: BBa_K266001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inverter TetR -> LuxR
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_J23100 constitutive promoter directs the expression of tetracycline repressor (TetR). TetR binds to pTet regulatory region resulting in a ''negative control'' of the production of LuxR. The whole system acts as an ''Inverter'' of Tet system controlling LuxR expression. TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc].
mutable notes: None
mutable provenance: The biobricks BBa_K145201 and BBa_K116640 where used for this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_K116640
component: BBa_K145201
sequence annotation: BBa_K116640
sequence annotation: BBa_K145201
sequence: https://synbiohub.org/public/igem/BBa_K266001_sequence/1

BBa_K116640
type: Component
persistent identity: component2287677
display id: component2287677
version: 1
top level: BBa_K266001
access: public
definition: https://synbiohub.org/public/igem/BBa_K116640/1

BBa_K145201
type: Component
persistent identity: component2287653
display id: component2287653
version: 1
top level: BBa_K266001
access: public
definition: https://synbiohub.org/public/igem/BBa_K145201/1

BBa_K116640
type: SequenceAnnotation
persistent identity: annotation2287677
display id: annotation2287677
version: 1
top level: BBa_K266001
location: https://synbiohub.org/public/igem/BBa_K266001/annotation2287677/range2287677/1
component: BBa_K116640

https://synbiohub.org/public/igem/BBa_K266001/annotation2287677/range2287677/1
type: Range
persistent identity: range2287677
display id: range2287677
version: 1
direction: direction/reverse
top level: BBa_K266001
start: 892
end: 1890
orientation: inline

BBa_K145201
type: SequenceAnnotation
persistent identity: annotation2287653
display id: annotation2287653
version: 1
top level: BBa_K266001
location: https://synbiohub.org/public/igem/BBa_K266001/annotation2287653/range2287653/1
component: BBa_K145201

https://synbiohub.org/public/igem/BBa_K266001/annotation2287653/range2287653/1
type: Range
persistent identity: range2287653
display id: range2287653
version: 1
direction: direction/reverse
top level: BBa_K266001
start: 1
end: 883
orientation: inline

BBa_K091104
type: ComponentDefinition
persistent identity: BBa K091104
display id: BBa_K091104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac/Mnt Hybrid Promoter
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to LacI. The promoter should be repressed by Mnt repressor. It should also be repressed by LacI, and in the absence of Mnt repressor, should be induced by IPTG.
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of a LacI binding site between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by LacI. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tgtgtggaattgtga, and 2) Position lacI binding site (composed of an inverted repeat) from the lacI regulated promoter (R0010) immediately 3??? to truncated mnt promoter.
mutable provenance: This part comes from the Mnt promoter and the LacI promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI
sequence annotation: Mnt
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K091104_sequence/1

LacI
type: SequenceAnnotation
persistent identity: annotation1963738
display id: annotation1963738
version: 1
top level: BBa_K091104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963738/range1963738/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963738/range1963738/1
type: Range
persistent identity: range1963738
display id: range1963738
version: 1
direction: direction/forward
top level: BBa_K091104
start: 53
end: 87
orientation: inline

Mnt
type: SequenceAnnotation
persistent identity: annotation1963743
display id: annotation1963743
version: 1
top level: BBa_K091104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963743/range1963743/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963743/range1963743/1
type: Range
persistent identity: range1963743
display id: range1963743
version: 1
direction: direction/forward
top level: BBa_K091104
start: 1
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963737
display id: annotation1963737
version: 1
top level: BBa_K091104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963737/range1963737/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963737/range1963737/1
type: Range
persistent identity: range1963737
display id: range1963737
version: 1
direction: direction/forward
top level: BBa_K091104
start: 47
end: 52
orientation: inline

BBa_K1833998
type: ComponentDefinition
persistent identity: BBa K1833998
display id: BBa_K1833998
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Repressible E. coli promoter as annealed oligos
creator: Konstantin Borisov
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T08:05:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a part used as a building block for E. coli driven expression that is repressible by a synthetic protein (for more information visit 2015.igem.org/Team:Pitt). In our project, we used this promoter to detect proteases that could cleave the synthetic repressor, and so we named the promoter "pProt". This part uses a special cloning method with the following synthesized nucleotides: pProtsense: aattcgcggccgcttctagattgacaaacaagatatcttaaatgaaaatacaagaaatcttaaatata pProtantisense: ctagtatatttaagatttcttgtattttcatttaagatatcttgtttgtcaatctagaagcggccgcg The following method can be used to insert pProt before a desired Biobrick part (which should contain an RBS at minimum and preferably a cds and terminator): 1. Cut the desired plasmid with EcoRI and XbaI. Purify the plasmid with a method of your choice (both gel purification and PCR purification kits from Qiagen serve to remove the short undesired oligonucleotide from the digestion). 2. Be sure that your oligos have 5' phosphorylation (treat unphosphorylated oligos with T4 Polynucleotide Kinase). Then, anneal the two oligos by slowly cooling from 98C to room temperature. 3. Finally, ligate the cut plasmid with the annealed oligos by using a 1:10 molar ratio of plasmid to insert. 4. Transform the ligation reaction into E. coli, and plate. 5. Pick colonies, and verify the success of the cloning with sequencing or PCR. (Doing a diagnostic digest is not recommended as the size of the original plasmid and the desired plasmid are very similar.) 6. Add a composite part to the iGEM registry by using this part with the original protein coding part, checking the box to create without a scar, as this part contains a modified scar.
mutable notes: When ligating with a cut plasmid, this leaves a modified Biobrick scar between the pProt promoter and original Biobrick part.
mutable provenance: Synthesized oligonucleotides as described on main page.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pProt
sequence annotation: Oligo scar
sequence: https://synbiohub.org/public/igem/BBa_K1833998_sequence/1

pProt
type: SequenceAnnotation
persistent identity: annotation2460155
display id: annotation2460155
version: 1
top level: BBa_K1833998
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1833998/annotation2460155/range2460155/1

https://synbiohub.org/public/igem/BBa_K1833998/annotation2460155/range2460155/1
type: Range
persistent identity: range2460155
display id: range2460155
version: 1
direction: direction/forward
top level: BBa_K1833998
start: 1
end: 47
orientation: inline

Oligo scar
type: SequenceAnnotation
persistent identity: annotation2460156
display id: annotation2460156
version: 1
top level: BBa_K1833998
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1833998/annotation2460156/range2460156/1

https://synbiohub.org/public/igem/BBa_K1833998/annotation2460156/range2460156/1
type: Range
persistent identity: range2460156
display id: range2460156
version: 1
direction: direction/forward
top level: BBa_K1833998
start: 48
end: 53
orientation: inline

BBa_J64067
type: ComponentDefinition
persistent identity: BBa J64067
display id: BBa_J64067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR+3OC6HSL independent R0065
creator: Jeffrey J Tabor
created: 2007-12-12T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The -35 box of R0065 has been modified to promote constitutive (LuxR+3OC6HSL-independent) gene expression. An extended -10 sequence has also been added to increase absolute transcription. The promoter still contains the operators OR1 and OR2 such that it retains its sensitivity to CI.
mutable notes: see above
mutable provenance: modified from R0065
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: lux box
sequence annotation: +1
sequence annotation: OR1
sequence annotation: extended -10
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J64067_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation1959023
display id: annotation1959023
version: 1
top level: BBa_J64067
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959023/range1959023/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959023/range1959023/1
type: Range
persistent identity: range1959023
display id: range1959023
version: 1
direction: direction/forward
top level: BBa_J64067
start: 58
end: 75
orientation: inline

lux box
type: SequenceAnnotation
persistent identity: annotation1959021
display id: annotation1959021
version: 1
top level: BBa_J64067
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959021/range1959021/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959021/range1959021/1
type: Range
persistent identity: range1959021
display id: range1959021
version: 1
direction: direction/forward
top level: BBa_J64067
start: 6
end: 26
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1959022
display id: annotation1959022
version: 1
top level: BBa_J64067
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959022/range1959022/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959022/range1959022/1
type: Range
persistent identity: range1959022
display id: range1959022
version: 1
direction: direction/forward
top level: BBa_J64067
start: 59
end: 59
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1959024
display id: annotation1959024
version: 1
top level: BBa_J64067
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959024/range1959024/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959024/range1959024/1
type: Range
persistent identity: range1959024
display id: range1959024
version: 1
direction: direction/forward
top level: BBa_J64067
start: 82
end: 98
orientation: inline

extended -10
type: SequenceAnnotation
persistent identity: annotation1959019
display id: annotation1959019
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959019/range1959019/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959019/range1959019/1
type: Range
persistent identity: range1959019
display id: range1959019
version: 1
direction: direction/forward
top level: BBa_J64067
start: 43
end: 46
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1959018
display id: annotation1959018
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959018/range1959018/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959018/range1959018/1
type: Range
persistent identity: range1959018
display id: range1959018
version: 1
direction: direction/forward
top level: BBa_J64067
start: 25
end: 30
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1959020
display id: annotation1959020
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959020/range1959020/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959020/range1959020/1
type: Range
persistent identity: range1959020
display id: range1959020
version: 1
direction: direction/forward
top level: BBa_J64067
start: 48
end: 53
orientation: inline

BBa_I731004
type: ComponentDefinition
persistent identity: BBa I731004
display id: BBa_I731004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA promoter
creator: Shaunak Vankudre
created: 2007-06-25T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _127_
m user id: 0
owner id: 1889
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: derived from Enz et al, 2003, J Bacteriol. 2003 April; 185(7): 2387???2391. doi: 10.1128/JB.185.7.2387-2391.2003.
mutable provenance: genomic sequence from E. Coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I731004_sequence/1

NAND59
type: ComponentDefinition
persistent identity: BBa I732305
display id: BBa_I732305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D059O46)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O55
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732305_sequence/1

BBa_K1165002
type: ComponentDefinition
persistent identity: BBa K1165002
display id: BBa_K1165002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 1 of XOR Gate 1
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the DNA sequence of a Y-type hammerhead ribozyme that targets and cleaves the PenI repressor (Part BBa_C0074). This ribozyme has target-binding flanking arms adjacent to the Y-type hammerhead catalytic core and synthetic extended arms located towards the 5' and 3' ends. The catalytic core allows for the sequence-specific cleavage of sites within the coding sequence of PenI. Hammerhead ribozymes are able to cleave at any NUH within an RNA sequence, where H represent any nucleotide except guanine (Mir 2001), although with GUC as the hammerhead consensus cleavage site (Lieber, 1995). The binding of the flanking arms adjacent to the hammerhead catalytic core favours the recognition and cleavage of only one target site. Additional secondary structure within the flanking arms was detected to a small degree by using IDT OligoAnalyzer's Hairpin tool (Owczarzy 2008) as is depicted below. [image] The synthetic extended arms are designed to bind to the complementary extended arms of another similar PenI-cleaving ribozyme (Part _____), but do not contribute to any additional secondary structure as confirmed via the Hairpin tool in IDT's OligoAnalyzer (Owczarzy 2008), as both arms of a particular ribozyme only contain a purine and pyrimidine pair that do not bind to each other (in this case, A and G). The two-nucleotide extended arms are also staggered in number to avoid slippage in binding and bind to its complement pair at significantly higher melting temperatures than the adjacent flanking arms. This feature allows for the sequestering of the two ribozymes when both are present and significantly decreasing any ribozyme activity. Regions of complementarity between the two target-binding regions of both ribozymes are also present to ensure that the functional catalytic cores are not able to cleave their mRNA targets, even when bound. The characteristics of this expressed pair of ribozymes were considered to create a functional RNA-based XOR logic gate. References Mir, A…
mutable notes: Although this particular part is likely to only cleave the PenI repressor mRNA due to its sequence specificity, the simple in silico design of this ribozyme allows for the cleavage of virtually any RNA sequence containing a cleave site (NUH). This particular sequence was obtained by first scanning our target PenI mRNA for the optimal cut site GUC. The 10 nucleotides surrounding this cut site were obtained and the complement of this area was generated for the ribozyme flanking arms. The length of this region was decided by ideal melting temperature (51.4 degrees Celsius). Additional synthetic extended arms were subsequently added to the 5' and 3' ends of the sequence for binding to the complement ribozyme in an XOR gate. This region of non-pairing purines and pyrimidines was staggered in base repeats to avoid slippage of binding and was defined in length by significantly higher melting temperatures (approximately 68 degrees Celsius). Finally, the catalytic core of the Y-type hammerhead ribozyme was introduced at the appropriate region. Throughout this entire process, secondary was continually checked and minimized using IDT OligoAnalyzer's Hairpin tool (Owczarzy 2008). The complete ribozyme sequences was also BLASTed again the E.coli genome, which was our chassis and yielded insignificant results. Other factors such as differential length or target binding regions due to varying melting temperature and GC content were also considered during the in silico design of this ribozyme and its pair in the XOR logic gate. References Owczarzy, R. et al. IDT SciTools: a suite for analysis and design of nucleic acid oligomers. Nucleic Acids Res 36, W163???W169 (2008).
mutable provenance: The target-binding flanking arms of the ribozyme were obtained from the complement of the PenI Repressor sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Target-Binding Arms
sequence annotation: Synthetic Extended Arms
sequence annotation: Catalytic Core
sequence annotation: TAA Bump
sequence: https://synbiohub.org/public/igem/BBa_K1165002_sequence/1

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364288
display id: annotation2364288
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364288/range2364288/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364288/range2364288/1
type: Range
persistent identity: range2364288
display id: range2364288
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 70
end: 81
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2364282
display id: annotation2364282
version: 1
top level: BBa_K1165002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364282/range2364282/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364282/range2364282/1
type: Range
persistent identity: range2364282
display id: range2364282
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 1
end: 30
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2364283
display id: annotation2364283
version: 1
top level: BBa_K1165002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364283/range2364283/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364283/range2364283/1
type: Range
persistent identity: range2364283
display id: range2364283
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 82
end: 111
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364284
display id: annotation2364284
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364284/range2364284/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364284/range2364284/1
type: Range
persistent identity: range2364284
display id: range2364284
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 31
end: 35
orientation: inline

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2364287
display id: annotation2364287
version: 1
top level: BBa_K1165002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364287/range2364287/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364287/range2364287/1
type: Range
persistent identity: range2364287
display id: range2364287
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 44
end: 69
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364285
display id: annotation2364285
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364285/range2364285/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364285/range2364285/1
type: Range
persistent identity: range2364285
display id: range2364285
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 39
end: 43
orientation: inline

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2364286
display id: annotation2364286
version: 1
top level: BBa_K1165002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364286/range2364286/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364286/range2364286/1
type: Range
persistent identity: range2364286
display id: range2364286
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 36
end: 38
orientation: inline

BBa_K121014
type: ComponentDefinition
persistent identity: BBa K121014
display id: BBa_K121014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lambda cI regulated)
creator: Masashi Kajita
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
group u list: _220_
m user id: 0
owner id: 3180
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: promoter (lambda cI regulated)
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OL3
sequence annotation: -35
sequence annotation: K121014
sequence annotation: OL1
sequence annotation: OL2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K121014_sequence/1

OL3
type: SequenceAnnotation
persistent identity: annotation1981847
display id: annotation1981847
version: 1
top level: BBa_K121014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981847/range1981847/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981847/range1981847/1
type: Range
persistent identity: range1981847
display id: range1981847
version: 1
direction: direction/forward
top level: BBa_K121014
start: 13
end: 29
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1981851
display id: annotation1981851
version: 1
top level: BBa_K121014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981851/range1981851/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981851/range1981851/1
type: Range
persistent identity: range1981851
display id: range1981851
version: 1
direction: direction/forward
top level: BBa_K121014
start: 47
end: 52
orientation: inline

K121014
type: SequenceAnnotation
persistent identity: annotation1981846
display id: annotation1981846
version: 1
top level: BBa_K121014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981846/range1981846/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981846/range1981846/1
type: Range
persistent identity: range1981846
display id: range1981846
version: 1
direction: direction/forward
top level: BBa_K121014
start: 1
end: 90
orientation: inline

OL1
type: SequenceAnnotation
persistent identity: annotation1981849
display id: annotation1981849
version: 1
top level: BBa_K121014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981849/range1981849/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981849/range1981849/1
type: Range
persistent identity: range1981849
display id: range1981849
version: 1
direction: direction/forward
top level: BBa_K121014
start: 57
end: 73
orientation: inline

OL2
type: SequenceAnnotation
persistent identity: annotation1981848
display id: annotation1981848
version: 1
top level: BBa_K121014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981848/range1981848/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981848/range1981848/1
type: Range
persistent identity: range1981848
display id: range1981848
version: 1
direction: direction/forward
top level: BBa_K121014
start: 33
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1981850
display id: annotation1981850
version: 1
top level: BBa_K121014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981850/range1981850/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981850/range1981850/1
type: Range
persistent identity: range1981850
display id: range1981850
version: 1
direction: direction/forward
top level: BBa_K121014
start: 70
end: 75
orientation: inline

BBa_J64068
type: ComponentDefinition
persistent identity: BBa J64068
display id: BBa_J64068
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: increased strength R0051
creator: Jeffrey J Tabor
created: 2008-01-11T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0051 was modified to contain consensus -35 and -10 sequences. The CI operator OR1 was moved from it's original position (overlapping the -10) to between the -35 and -10. Fortuitously this results in the optimal (for high expression) 17bp spacing between the -35 and -10 sites.
mutable notes: none
mutable provenance: designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J64068_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1959540
display id: annotation1959540
version: 1
top level: BBa_J64068
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64068/annotation1959540/range1959540/1

https://synbiohub.org/public/igem/BBa_J64068/annotation1959540/range1959540/1
type: Range
persistent identity: range1959540
display id: range1959540
version: 1
direction: direction/forward
top level: BBa_J64068
start: 21
end: 37
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1959541
display id: annotation1959541
version: 1
top level: BBa_J64068
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64068/annotation1959541/range1959541/1

https://synbiohub.org/public/igem/BBa_J64068/annotation1959541/range1959541/1
type: Range
persistent identity: range1959541
display id: range1959541
version: 1
direction: direction/forward
top level: BBa_J64068
start: 38
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1959539
display id: annotation1959539
version: 1
top level: BBa_J64068
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64068/annotation1959539/range1959539/1

https://synbiohub.org/public/igem/BBa_J64068/annotation1959539/range1959539/1
type: Range
persistent identity: range1959539
display id: range1959539
version: 1
direction: direction/forward
top level: BBa_J64068
start: 15
end: 20
orientation: inline

BBa_K1520025
type: ComponentDefinition
persistent identity: BBa K1520025
display id: BBa_K1520025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter. It produces rfp when there is enough lacI or less lactose.
mutable notes: None
mutable provenance: Ptet, rfp, Plac, tetR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520015
component: BBa_K1520014
sequence annotation: BBa_K1520014
sequence annotation: BBa_K1520015
sequence: https://synbiohub.org/public/igem/BBa_K1520025_sequence/1

BBa_K1520015
type: Component
persistent identity: component2385284
display id: component2385284
version: 1
top level: BBa_K1520025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520015/1

BBa_K1520014
type: Component
persistent identity: component2385272
display id: component2385272
version: 1
top level: BBa_K1520025
access: public
definition: BBa_K1520014

BBa_K1520014
type: SequenceAnnotation
persistent identity: annotation2385272
display id: annotation2385272
version: 1
top level: BBa_K1520025
location: https://synbiohub.org/public/igem/BBa_K1520025/annotation2385272/range2385272/1
component: BBa_K1520014

https://synbiohub.org/public/igem/BBa_K1520025/annotation2385272/range2385272/1
type: Range
persistent identity: range2385272
display id: range2385272
version: 1
direction: direction/reverse
top level: BBa_K1520025
start: 1
end: 931
orientation: inline

BBa_K1520015
type: SequenceAnnotation
persistent identity: annotation2385284
display id: annotation2385284
version: 1
top level: BBa_K1520025
location: https://synbiohub.org/public/igem/BBa_K1520025/annotation2385284/range2385284/1
component: BBa_K1520015

https://synbiohub.org/public/igem/BBa_K1520025/annotation2385284/range2385284/1
type: Range
persistent identity: range2385284
display id: range2385284
version: 1
direction: direction/reverse
top level: BBa_K1520025
start: 940
end: 1987
orientation: inline

BBa_K137124
type: ComponentDefinition
persistent identity: BBa K137124
display id: BBa_K137124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI-repressed promoter A81
creator: Josh Michener
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: true
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 253
owning group id: 6
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: LacI-repressible promoter A81 developed by Sidney Cox (http://www.nature.com/msb/journal/v3/n1/full/msb4100187.html). Showed a regulatory range (ON/OFF) of 1.5x10^4.
mutable provenance: Annealed from oligos.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137124_sequence/1

LacI:CspA
type: ComponentDefinition
persistent identity: BBa K538203
display id: BBa_K538203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + CspA
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_K538003
component: BBa_B0034
sequence annotation: BBa_K538003
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K538203_sequence/1

BBa_R0010
type: Component
persistent identity: component2134058
display id: component2134058
version: 1
top level: LacI:CspA
access: public
definition: LacI

BBa_K538003
type: Component
persistent identity: component2134071
display id: component2134071
version: 1
top level: LacI:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_K538003/1

BBa_B0034
type: Component
persistent identity: component2134066
display id: component2134066
version: 1
top level: LacI:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538003
type: SequenceAnnotation
persistent identity: annotation2134071
display id: annotation2134071
version: 1
top level: LacI:CspA
location: https://synbiohub.org/public/igem/BBa_K538203/annotation2134071/range2134071/1
component: BBa_K538003

https://synbiohub.org/public/igem/BBa_K538203/annotation2134071/range2134071/1
type: Range
persistent identity: range2134071
display id: range2134071
version: 1
direction: direction/reverse
top level: LacI:CspA
start: 227
end: 421
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134066
display id: annotation2134066
version: 1
top level: LacI:CspA
location: https://synbiohub.org/public/igem/BBa_K538203/annotation2134066/range2134066/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538203/annotation2134066/range2134066/1
type: Range
persistent identity: range2134066
display id: range2134066
version: 1
direction: direction/reverse
top level: LacI:CspA
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2134058
display id: annotation2134058
version: 1
top level: LacI:CspA
location: https://synbiohub.org/public/igem/BBa_K538203/annotation2134058/range2134058/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538203/annotation2134058/range2134058/1
type: Range
persistent identity: range2134058
display id: range2134058
version: 1
direction: direction/forward
top level: LacI:CspA
start: 1
end: 200
orientation: inline

BBa_K598000
type: ComponentDefinition
persistent identity: BBa K598000
display id: BBa_K598000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+E0040+B0015
creator: Yangyang ZHAO, Yan GONG, Zhenrun ZHANG, Qingyang XIAO
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 4411
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is TPP up-regulated hammerhead ribozyme, consisting of 131 nucleotides.
mutable notes: This part contains a native ribosomal binding site
mutable provenance: This part was an engineered RNA controller composed of a TPP aptamer from B.subtilis and a hammerhead motif.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: TPP Ribozyme 2.5
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: T7 TE
sequence annotation: native RBS
sequence annotation: stem_loop
sequence annotation: polya
sequence annotation: BBa_B0015
sequence annotation: eGFP
sequence: https://synbiohub.org/public/igem/BBa_K598000_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2152872
display id: annotation2152872
version: 1
top level: BBa_K598000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152872/range2152872/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152872/range2152872/1
type: Range
persistent identity: range2152872
display id: range2152872
version: 1
direction: direction/forward
top level: BBa_K598000
start: 1000
end: 1000
orientation: inline

TPP Ribozyme 2.5
type: SequenceAnnotation
persistent identity: annotation2152875
display id: annotation2152875
version: 1
top level: BBa_K598000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152875/range2152875/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152875/range2152875/1
type: Range
persistent identity: range2152875
display id: range2152875
version: 1
direction: direction/forward
top level: BBa_K598000
start: 1
end: 150
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2152836
display id: annotation2152836
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152836/range2152836/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152836/range2152836/1
type: Range
persistent identity: range2152836
display id: range2152836
version: 1
direction: direction/forward
top level: BBa_K598000
start: 879
end: 958
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2152867
display id: annotation2152867
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152867/range2152867/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152867/range2152867/1
type: Range
persistent identity: range2152867
display id: range2152867
version: 1
direction: direction/forward
top level: BBa_K598000
start: 967
end: 1007
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2152880
display id: annotation2152880
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152880/range2152880/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152880/range2152880/1
type: Range
persistent identity: range2152880
display id: range2152880
version: 1
direction: direction/forward
top level: BBa_K598000
start: 151
end: 870
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152868
display id: annotation2152868
version: 1
top level: BBa_K598000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152868/range2152868/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152868/range2152868/1
type: Range
persistent identity: range2152868
display id: range2152868
version: 1
direction: direction/forward
top level: BBa_K598000
start: 974
end: 993
orientation: inline

native RBS
type: SequenceAnnotation
persistent identity: annotation2131876
display id: annotation2131876
version: 1
top level: BBa_K598000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2131876/range2131876/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2131876/range2131876/1
type: Range
persistent identity: range2131876
display id: range2131876
version: 1
direction: direction/forward
top level: BBa_K598000
start: 138
end: 145
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2152857
display id: annotation2152857
version: 1
top level: BBa_K598000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152857/range2152857/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152857/range2152857/1
type: Range
persistent identity: range2152857
display id: range2152857
version: 1
direction: direction/forward
top level: BBa_K598000
start: 890
end: 933
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2152870
display id: annotation2152870
version: 1
top level: BBa_K598000
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152870/range2152870/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152870/range2152870/1
type: Range
persistent identity: range2152870
display id: range2152870
version: 1
direction: direction/forward
top level: BBa_K598000
start: 994
end: 1007
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153033
display id: annotation2153033
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2153033/range2153033/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2153033/range2153033/1
type: Range
persistent identity: range2153033
display id: range2153033
version: 1
direction: direction/forward
top level: BBa_K598000
start: 879
end: 1007
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2152835
display id: annotation2152835
version: 1
top level: BBa_K598000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152835/range2152835/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152835/range2152835/1
type: Range
persistent identity: range2152835
display id: range2152835
version: 1
direction: direction/forward
top level: BBa_K598000
start: 151
end: 870
orientation: inline

BBa_K266007
type: ComponentDefinition
persistent identity: BBa K266007
display id: BBa_K266007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Complex QS -> LuxI & LasI circuit
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Complex Quorum sensing circuit that receives the signal of PAI+LasR and AI+LuxR to control the production of LuxI and LasI enzymes.
mutable notes: None
mutable provenance: The biobricks BBa_K266006 and BBa_K266000 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K266000
component: BBa_K266006
sequence annotation: BBa_K266006
sequence annotation: BBa_K266000
sequence: https://synbiohub.org/public/igem/BBa_K266007_sequence/1

BBa_K266000
type: Component
persistent identity: component2297452
display id: component2297452
version: 1
top level: BBa_K266007
access: public
definition: https://synbiohub.org/public/igem/BBa_K266000/1

BBa_K266006
type: Component
persistent identity: component2297433
display id: component2297433
version: 1
top level: BBa_K266007
access: public
definition: BBa_K266006

BBa_K266006
type: SequenceAnnotation
persistent identity: annotation2297433
display id: annotation2297433
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
component: BBa_K266006

https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
type: Range
persistent identity: range2297433
display id: range2297433
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1
end: 1705
orientation: inline

BBa_K266000
type: SequenceAnnotation
persistent identity: annotation2297452
display id: annotation2297452
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
component: BBa_K266000

https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
type: Range
persistent identity: range2297452
display id: range2297452
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1714
end: 2676
orientation: inline

BBa_I718018
type: ComponentDefinition
persistent identity: BBa I718018
display id: BBa_I718018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dapAp promoter
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
group u list: _141_
m user id: 0
owner id: 1568
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: dapAp is the promter of dapA gene in E.coli. DapA enzyme is part of diaminopimelate (DAP)biosynthesis pathway. DAP is necessary for peptidoglycan and lysine biosynthesis. dapAp prmoter has been previously studied: Acord04: Acord J, Masters M (2004). "Expression from the Escherichia coli dapA promoter is regulated by intracellular levels of diaminopimelic acid." FEMS Microbiol Lett 235(1);131-7. PMID: 15158272 It has been shown that dapAp driven expression is inhibited at hight DAP. We have tried to confirm this by
mutable notes: None
mutable provenance: none yet
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I718018_sequence/1

LacI:SDnaK
type: ComponentDefinition
persistent identity: BBa K538202
display id: BBa_K538202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + SheDnaK
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_B0034
component: BBa_K538002
sequence annotation: BBa_R0010
sequence annotation: BBa_K538002
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K538202_sequence/1

BBa_R0010
type: Component
persistent identity: component2126742
display id: component2126742
version: 1
top level: LacI:SDnaK
access: public
definition: LacI

BBa_B0034
type: Component
persistent identity: component2126750
display id: component2126750
version: 1
top level: LacI:SDnaK
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538002
type: Component
persistent identity: component2126753
display id: component2126753
version: 1
top level: LacI:SDnaK
access: public
definition: https://synbiohub.org/public/igem/BBa_K538002/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2126742
display id: annotation2126742
version: 1
top level: LacI:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538202/annotation2126742/range2126742/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538202/annotation2126742/range2126742/1
type: Range
persistent identity: range2126742
display id: range2126742
version: 1
direction: direction/forward
top level: LacI:SDnaK
start: 1
end: 200
orientation: inline

BBa_K538002
type: SequenceAnnotation
persistent identity: annotation2126753
display id: annotation2126753
version: 1
top level: LacI:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538202/annotation2126753/range2126753/1
component: BBa_K538002

https://synbiohub.org/public/igem/BBa_K538202/annotation2126753/range2126753/1
type: Range
persistent identity: range2126753
display id: range2126753
version: 1
direction: direction/reverse
top level: LacI:SDnaK
start: 227
end: 2146
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2126750
display id: annotation2126750
version: 1
top level: LacI:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538202/annotation2126750/range2126750/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538202/annotation2126750/range2126750/1
type: Range
persistent identity: range2126750
display id: range2126750
version: 1
direction: direction/reverse
top level: LacI:SDnaK
start: 209
end: 220
orientation: inline

BBa_K332033
type: ComponentDefinition
persistent identity: BBa K332033
display id: BBa_K332033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It is a transcriptional unit including 37℃ induced RBS + tetR + double terminator. It can be added various promoter to adapt your conditions.
mutable notes: To regulate following genes by temperature. To maintain cells at 37?? C ↑ : the tetR protein be generated and repress Ptet's circuit. To release cells at 37?? C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
mutable provenance: the 37℃ induced RBS cames from iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0040
component: BBa_K115002
component: BBa_J61048
sequence annotation: BBa_J61048
sequence annotation: BBa_C0040
sequence annotation: BBa_K115002
sequence: https://synbiohub.org/public/igem/BBa_K332033_sequence/1

BBa_C0040
type: Component
persistent identity: component2247615
display id: component2247615
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_K115002
type: Component
persistent identity: component2247611
display id: component2247611
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_J61048
type: Component
persistent identity: component2247616
display id: component2247616
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2247616
display id: annotation2247616
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
type: Range
persistent identity: range2247616
display id: range2247616
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 752
end: 864
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2247615
display id: annotation2247615
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
type: Range
persistent identity: range2247615
display id: range2247615
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 59
end: 743
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2247611
display id: annotation2247611
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
type: Range
persistent identity: range2247611
display id: range2247611
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 1
end: 52
orientation: inline

Cpn10/60
type: ComponentDefinition
persistent identity: BBa K538210
display id: BBa_K538210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cpn10/60 operon (pLacI)
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K538001
component: BBa_B0034
component: BBa_R0010
component: BBa_K538000
sequence annotation: BBa_K538000
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_K538001
sequence: https://synbiohub.org/public/igem/BBa_K538210_sequence/1

BBa_K538001
type: Component
persistent identity: component2222773
display id: component2222773
version: 1
top level: Cpn10/60
access: public
definition: https://synbiohub.org/public/igem/BBa_K538001/1

BBa_B0034
type: Component
persistent identity: component2222764
display id: component2222764
version: 1
top level: Cpn10/60
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component2222756
display id: component2222756
version: 1
top level: Cpn10/60
access: public
definition: LacI

BBa_B0034
type: Component
persistent identity: component2222769
display id: component2222769
version: 1
top level: Cpn10/60
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538000
type: Component
persistent identity: component2222767
display id: component2222767
version: 1
top level: Cpn10/60
access: public
definition: https://synbiohub.org/public/igem/BBa_K538000/1

BBa_K538000
type: SequenceAnnotation
persistent identity: annotation2222767
display id: annotation2222767
version: 1
top level: Cpn10/60
location: https://synbiohub.org/public/igem/BBa_K538210/annotation2222767/range2222767/1
component: BBa_K538000

https://synbiohub.org/public/igem/BBa_K538210/annotation2222767/range2222767/1
type: Range
persistent identity: range2222767
display id: range2222767
version: 1
direction: direction/reverse
top level: Cpn10/60
start: 227
end: 523
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2222764
display id: annotation2222764
version: 1
top level: Cpn10/60
location: https://synbiohub.org/public/igem/BBa_K538210/annotation2222764/range2222764/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538210/annotation2222764/range2222764/1
type: Range
persistent identity: range2222764
display id: range2222764
version: 1
direction: direction/reverse
top level: Cpn10/60
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2222756
display id: annotation2222756
version: 1
top level: Cpn10/60
location: https://synbiohub.org/public/igem/BBa_K538210/annotation2222756/range2222756/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538210/annotation2222756/range2222756/1
type: Range
persistent identity: range2222756
display id: range2222756
version: 1
direction: direction/forward
top level: Cpn10/60
start: 1
end: 200
orientation: inline

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2222773
display id: annotation2222773
version: 1
top level: Cpn10/60
location: https://synbiohub.org/public/igem/BBa_K538210/annotation2222773/range2222773/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538210/annotation2222773/range2222773/1
type: Range
persistent identity: range2222773
display id: range2222773
version: 1
direction: direction/reverse
top level: Cpn10/60
start: 550
end: 2199
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2222769
display id: annotation2222769
version: 1
top level: Cpn10/60
location: https://synbiohub.org/public/igem/BBa_K538210/annotation2222769/range2222769/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538210/annotation2222769/range2222769/1
type: Range
persistent identity: range2222769
display id: range2222769
version: 1
direction: direction/reverse
top level: Cpn10/60
start: 532
end: 543
orientation: inline

BBa_K086019
type: ComponentDefinition
persistent identity: BBa K086019
display id: BBa_K086019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: Lac O and sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_2
sequence: https://synbiohub.org/public/igem/BBa_K086019_sequence/1

P24_2
type: SequenceAnnotation
persistent identity: annotation1995753
display id: annotation1995753
version: 1
top level: BBa_K086019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086019/annotation1995753/range1995753/1

https://synbiohub.org/public/igem/BBa_K086019/annotation1995753/range1995753/1
type: Range
persistent identity: range1995753
display id: range1995753
version: 1
direction: direction/forward
top level: BBa_K086019
start: 1
end: 55
orientation: inline

glnAp2 pro
type: ComponentDefinition
persistent identity: BBa J64979
display id: BBa_J64979
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glnAp2
creator: Kristopher Kuchenbecker
created: 2007-03-25T12:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter sequence (-80 to +1) is responsive to the transcription enhancer glnG.
mutable notes: n/a
mutable provenance: ''E. coli''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J64979_sequence/1

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732444
display id: BBa_I732444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732444_sequence/1

BBa_R2112
type: ComponentDefinition
persistent identity: BBa R2112
display id: BBa_R2112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was rationally designed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 site
sequence annotation: -10 site
sequence annotation: transcription start site
sequence annotation: C2003 operator site
sequence: https://synbiohub.org/public/igem/BBa_R2112_sequence/1

-35 site
type: SequenceAnnotation
persistent identity: annotation1903924
display id: annotation1903924
version: 1
top level: BBa_R2112
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903924/range1903924/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903924/range1903924/1
type: Range
persistent identity: range1903924
display id: range1903924
version: 1
direction: direction/forward
top level: BBa_R2112
start: 17
end: 22
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903925
display id: annotation1903925
version: 1
top level: BBa_R2112
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903925/range1903925/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903925/range1903925/1
type: Range
persistent identity: range1903925
display id: range1903925
version: 1
direction: direction/forward
top level: BBa_R2112
start: 40
end: 45
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903926
display id: annotation1903926
version: 1
top level: BBa_R2112
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903926/range1903926/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903926/range1903926/1
type: Range
persistent identity: range1903926
display id: range1903926
version: 1
direction: direction/forward
top level: BBa_R2112
start: 53
end: 53
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1903927
display id: annotation1903927
version: 1
top level: BBa_R2112
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903927/range1903927/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903927/range1903927/1
type: Range
persistent identity: range1903927
display id: range1903927
version: 1
direction: direction/forward
top level: BBa_R2112
start: 31
end: 42
orientation: inline

Pxyl
type: ComponentDefinition
persistent identity: BBa K143014
display id: BBa_K143014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Xyl for B.subtilis
creator: James Chappell
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter Xylose is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give '''Polymerase Per Second'''(PoPS) as an output. Promoter xylose is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give '''Polymerase Per Second'''(PoPS) as an output. Xylose does not induce the promoter xylose directly, but requires the transcriptional regulator '''XylR''', ( BBa_K143036 ) This means that XylR must be constitutively expressed in ''B.subtilis'' in order to use the promoter xylose.
mutable notes: Biobrick standard was applied to the promoter xylose sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 12. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: XylR Operator
sequence annotation: Sigma A -10
sequence annotation: Sigma A -35
sequence: https://synbiohub.org/public/igem/BBa_K143014_sequence/1

XylR Operator
type: SequenceAnnotation
persistent identity: annotation1975868
display id: annotation1975868
version: 1
top level: Pxyl
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1975868/range1975868/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1975868/range1975868/1
type: Range
persistent identity: range1975868
display id: range1975868
version: 1
direction: direction/forward
top level: Pxyl
start: 51
end: 61
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1976429
display id: annotation1976429
version: 1
top level: Pxyl
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1976429/range1976429/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1976429/range1976429/1
type: Range
persistent identity: range1976429
display id: range1976429
version: 1
direction: direction/forward
top level: Pxyl
start: 36
end: 41
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1976428
display id: annotation1976428
version: 1
top level: Pxyl
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1976428/range1976428/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1976428/range1976428/1
type: Range
persistent identity: range1976428
display id: range1976428
version: 1
direction: direction/forward
top level: Pxyl
start: 13
end: 18
orientation: inline

XylR Operator
type: SequenceAnnotation
persistent identity: annotation1975869
display id: annotation1975869
version: 1
top level: Pxyl
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1975869/range1975869/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1975869/range1975869/1
type: Range
persistent identity: range1975869
display id: range1975869
version: 1
direction: direction/forward
top level: Pxyl
start: 65
end: 75
orientation: inline

BBa_K1520014
type: ComponentDefinition
persistent identity: BBa K1520014
display id: BBa_K1520014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ptet-rbs-rfp-Ter. It produces rfp when there is less tetR.
mutable notes: None
mutable provenance: Ptet, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1520500
component: BBa_K1520029
component: BBa_R0040
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520500
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1520014_sequence/1

BBa_K1520500
type: Component
persistent identity: component2384997
display id: component2384997
version: 1
top level: BBa_K1520014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520029
type: Component
persistent identity: component2384998
display id: component2384998
version: 1
top level: BBa_K1520014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0040
type: Component
persistent identity: component2384992
display id: component2384992
version: 1
top level: BBa_K1520014
access: public
definition: p(tetR)

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384998
display id: annotation2384998
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384998/range2384998/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384998/range2384998/1
type: Range
persistent identity: range2384998
display id: range2384998
version: 1
direction: direction/reverse
top level: BBa_K1520014
start: 803
end: 931
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384997
display id: annotation2384997
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384997/range2384997/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384997/range2384997/1
type: Range
persistent identity: range2384997
display id: range2384997
version: 1
direction: direction/reverse
top level: BBa_K1520014
start: 63
end: 794
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384992
display id: annotation2384992
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384992/range2384992/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384992/range2384992/1
type: Range
persistent identity: range2384992
display id: range2384992
version: 1
direction: direction/forward
top level: BBa_K1520014
start: 1
end: 54
orientation: inline

BBa_K112406
type: ComponentDefinition
persistent identity: BBa K112406
display id: BBa_K112406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cadC promoter
creator: Bing Xia
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Biobricking of cadC promoter and cadC gene PCR cadC-F and cadC-R on MG1655 (2336 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112406 ---------------------------- cb10013-cadC-F cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc cb10012-cad-R cgtagGGATCCtgaccagaccaaactggcg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112406_sequence/1

BBa_K1682004
type: ComponentDefinition
persistent identity: BBa K1682004
display id: BBa_K1682004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>G]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:16:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>G]
mutable notes: kdpFp[-15, T>G]
mutable provenance: kdpFp[-15, T>G]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: EcoRI Illegal site removed T->G
sequence annotation: phospho-KdpE binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K1682004_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2473582
display id: annotation2473582
version: 1
top level: BBa_K1682004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473582/range2473582/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473582/range2473582/1
type: Range
persistent identity: range2473582
display id: range2473582
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 69
end: 74
orientation: inline

EcoRI Illegal site removed T->G
type: SequenceAnnotation
persistent identity: annotation2475215
display id: annotation2475215
version: 1
top level: BBa_K1682004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2475215/range2475215/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2475215/range2475215/1
type: Range
persistent identity: range2475215
display id: range2475215
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 65
end: 66
orientation: inline

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473581
display id: annotation2473581
version: 1
top level: BBa_K1682004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473581/range2473581/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473581/range2473581/1
type: Range
persistent identity: range2473581
display id: range2473581
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 8
end: 24
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473584
display id: annotation2473584
version: 1
top level: BBa_K1682004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473584/range2473584/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473584/range2473584/1
type: Range
persistent identity: range2473584
display id: range2473584
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 42
end: 47
orientation: inline

BBa_K086020
type: ComponentDefinition
persistent identity: BBa K086020
display id: BBa_K086020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: Lac O and sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_3
sequence: https://synbiohub.org/public/igem/BBa_K086020_sequence/1

P24_3
type: SequenceAnnotation
persistent identity: annotation1995754
display id: annotation1995754
version: 1
top level: BBa_K086020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086020/annotation1995754/range1995754/1

https://synbiohub.org/public/igem/BBa_K086020/annotation1995754/range1995754/1
type: Range
persistent identity: range1995754
display id: range1995754
version: 1
direction: direction/forward
top level: BBa_K086020
start: 1
end: 55
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732403
display id: BBa_I732403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D062NUL)
creator: Zhan Jian
created: 2007-09-20T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Might be later edited.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O1
sequence annotation: +1
sequence annotation: consensus elements
sequence: https://synbiohub.org/public/igem/BBa_I732403_sequence/1

O1
type: SequenceAnnotation
persistent identity: annotation1944543
display id: annotation1944543
version: 1
top level: U073O11+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944543/range1944543/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944543/range1944543/1
type: Range
persistent identity: range1944543
display id: range1944543
version: 1
direction: direction/forward
top level: U073O11+D0
start: 1
end: 20
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944544
display id: annotation1944544
version: 1
top level: U073O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944544/range1944544/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944544/range1944544/1
type: Range
persistent identity: range1944544
display id: range1944544
version: 1
direction: direction/forward
top level: U073O11+D0
start: 93
end: 93
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944542
display id: annotation1944542
version: 1
top level: U073O11+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944542/range1944542/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944542/range1944542/1
type: Range
persistent identity: range1944542
display id: range1944542
version: 1
direction: direction/forward
top level: U073O11+D0
start: 57
end: 93
orientation: inline

BBa_I732276
type: ComponentDefinition
persistent identity: BBa I732276
display id: BBa_I732276
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O27)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732276_sequence/1

BBa_I714924
type: ComponentDefinition
persistent identity: BBa I714924
display id: BBa_I714924
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RecA_DlexO_DLacO1
creator: Chunbo Lou
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a NOR gate
mutable notes: NOR gat
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: SDY_EGFP
sequence annotation: LexA
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacO1
sequence: https://synbiohub.org/public/igem/BBa_I714924_sequence/1

SDY_EGFP
type: SequenceAnnotation
persistent identity: annotation1956422
display id: annotation1956422
version: 1
top level: BBa_I714924
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1956422/range1956422/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1956422/range1956422/1
type: Range
persistent identity: range1956422
display id: range1956422
version: 1
direction: direction/forward
top level: BBa_I714924
start: 121
end: 862
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation1956155
display id: annotation1956155
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1956155/range1956155/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1956155/range1956155/1
type: Range
persistent identity: range1956155
display id: range1956155
version: 1
direction: direction/forward
top level: BBa_I714924
start: 35
end: 55
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation1951922
display id: annotation1951922
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951922/range1951922/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951922/range1951922/1
type: Range
persistent identity: range1951922
display id: range1951922
version: 1
direction: direction/forward
top level: BBa_I714924
start: 65
end: 80
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1951925
display id: annotation1951925
version: 1
top level: BBa_I714924
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951925/range1951925/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951925/range1951925/1
type: Range
persistent identity: range1951925
display id: range1951925
version: 1
direction: direction/forward
top level: BBa_I714924
start: 59
end: 64
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1951926
display id: annotation1951926
version: 1
top level: BBa_I714924
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951926/range1951926/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951926/range1951926/1
type: Range
persistent identity: range1951926
display id: range1951926
version: 1
direction: direction/forward
top level: BBa_I714924
start: 81
end: 86
orientation: inline

LacO1
type: SequenceAnnotation
persistent identity: annotation1951921
display id: annotation1951921
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951921/range1951921/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951921/range1951921/1
type: Range
persistent identity: range1951921
display id: range1951921
version: 1
direction: direction/forward
top level: BBa_I714924
start: 1
end: 21
orientation: inline

LacO1
type: SequenceAnnotation
persistent identity: annotation1951924
display id: annotation1951924
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951924/range1951924/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951924/range1951924/1
type: Range
persistent identity: range1951924
display id: range1951924
version: 1
direction: direction/forward
top level: BBa_I714924
start: 93
end: 112
orientation: inline

BBa_K094120
type: ComponentDefinition
persistent identity: BBa K094120
display id: BBa_K094120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI/ara-1
creator: Shi Lei
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.
mutable notes: IPTG has better effect on this promoter than arabinose
mutable provenance: Standard pLacI/ara-1 promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pLac/ara-1
sequence: https://synbiohub.org/public/igem/BBa_K094120_sequence/1

pLac/ara-1
type: SequenceAnnotation
persistent identity: annotation1980631
display id: annotation1980631
version: 1
top level: BBa_K094120
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K094120/annotation1980631/range1980631/1

https://synbiohub.org/public/igem/BBa_K094120/annotation1980631/range1980631/1
type: Range
persistent identity: range1980631
display id: range1980631
version: 1
direction: direction/forward
top level: BBa_K094120
start: 1
end: 103
orientation: inline

BBa_K145152
type: ComponentDefinition
persistent identity: BBa K145152
display id: BBa_K145152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter: P22 c2 , LacI NOR gate
creator: Jonas Demeulemeester
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Hybrid promoter constisting of two LacI O2 sites at the ends of the promoter and two recognition sites (OR2 and OR1) for the P22 c2 repressor. The promoter should function as a NOR gate.
mutable notes: a small fragment of OR3 remains.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O2 P22 c2
sequence annotation: O2 LacI
sequence annotation: O1 P22 c2
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K145152_sequence/1

O2 P22 c2
type: SequenceAnnotation
persistent identity: annotation1971581
display id: annotation1971581
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971581/range1971581/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971581/range1971581/1
type: Range
persistent identity: range1971581
display id: range1971581
version: 1
direction: direction/forward
top level: BBa_K145152
start: 59
end: 76
orientation: inline

O2 LacI
type: SequenceAnnotation
persistent identity: annotation1971580
display id: annotation1971580
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971580/range1971580/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971580/range1971580/1
type: Range
persistent identity: range1971580
display id: range1971580
version: 1
direction: direction/forward
top level: BBa_K145152
start: 115
end: 135
orientation: inline

O2 LacI
type: SequenceAnnotation
persistent identity: annotation1971579
display id: annotation1971579
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971579/range1971579/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971579/range1971579/1
type: Range
persistent identity: range1971579
display id: range1971579
version: 1
direction: direction/forward
top level: BBa_K145152
start: 1
end: 21
orientation: inline

O1 P22 c2
type: SequenceAnnotation
persistent identity: annotation1971587
display id: annotation1971587
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971587/range1971587/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971587/range1971587/1
type: Range
persistent identity: range1971587
display id: range1971587
version: 1
direction: direction/forward
top level: BBa_K145152
start: 82
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1971558
display id: annotation1971558
version: 1
top level: BBa_K145152
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971558/range1971558/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971558/range1971558/1
type: Range
persistent identity: range1971558
display id: range1971558
version: 1
direction: direction/forward
top level: BBa_K145152
start: 78
end: 83
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1971557
display id: annotation1971557
version: 1
top level: BBa_K145152
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971557/range1971557/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971557/range1971557/1
type: Range
persistent identity: range1971557
display id: range1971557
version: 1
direction: direction/forward
top level: BBa_K145152
start: 56
end: 61
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732426
display id: BBa_I732426
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732426_sequence/1

BBa_J54130
type: ComponentDefinition
persistent identity: BBa J54130
display id: BBa_J54130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BetI_regulated promoter
creator: Yusaku Nakashima
created: 2006-10-20T11:00:00Z
modified: 2015-08-31T03:48:12Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BetI_regulated promoter
mutable notes: As BetI regulated
mutable provenance: DNA synthsis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BetI_regulated promoter
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_J54130_sequence/1

BetI_regulated promoter
type: SequenceAnnotation
persistent identity: annotation1903812
display id: annotation1903812
version: 1
top level: BBa_J54130
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J54130/annotation1903812/range1903812/1

https://synbiohub.org/public/igem/BBa_J54130/annotation1903812/range1903812/1
type: Range
persistent identity: range1903812
display id: range1903812
version: 1
direction: direction/forward
top level: BBa_J54130
start: 6
end: 43
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1903813
display id: annotation1903813
version: 1
top level: BBa_J54130
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54130/annotation1903813/range1903813/1

https://synbiohub.org/public/igem/BBa_J54130/annotation1903813/range1903813/1
type: Range
persistent identity: range1903813
display id: range1903813
version: 1
direction: direction/forward
top level: BBa_J54130
start: 1
end: 5
orientation: inline

BBa_J64800
type: ComponentDefinition
persistent identity: BBa J64800
display id: BBa_J64800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RHLR/RHLI Inducible & LasR/LasI repressible Promoter
creator: laura lavery
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLi binding.
mutable notes: There is a 17bp spacer between the -35 and -10 regions and the binding site for RHLR/RHLI is 20bp long. I was able to fit the whole binding site in the spacer region because the last three base pairs of the -35 region and the first three base pairs of the RHLR/RHLI binding site are the same.
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LasR/LasI
sequence annotation: -10
sequence annotation: -35
sequence annotation: RHLR/RHLI
sequence: https://synbiohub.org/public/igem/BBa_J64800_sequence/1

LasR/LasI
type: SequenceAnnotation
persistent identity: annotation1921061
display id: annotation1921061
version: 1
top level: BBa_J64800
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1
type: Range
persistent identity: range1921061
display id: range1921061
version: 1
direction: direction/forward
top level: BBa_J64800
start: 23
end: 40
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921063
display id: annotation1921063
version: 1
top level: BBa_J64800
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1
type: Range
persistent identity: range1921063
display id: range1921063
version: 1
direction: direction/forward
top level: BBa_J64800
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921062
display id: annotation1921062
version: 1
top level: BBa_J64800
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1
type: Range
persistent identity: range1921062
display id: range1921062
version: 1
direction: direction/forward
top level: BBa_J64800
start: 19
end: 24
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921060
display id: annotation1921060
version: 1
top level: BBa_J64800
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1
type: Range
persistent identity: range1921060
display id: range1921060
version: 1
direction: direction/forward
top level: BBa_J64800
start: 1
end: 23
orientation: inline

BBa_J34800
type: ComponentDefinition
persistent identity: BBa J34800
display id: BBa_J34800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter tetracyclin inducible
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: promoter
mutable notes: no
mutable provenance: no
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34800_sequence/1

BBa_K101001
type: ComponentDefinition
persistent identity: BBa K101001
display id: BBa_K101001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for LacI and LambdacI
creator: Bennett Swiniarski
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a new construct of a promoter that is dually repressed by either LacI or LambdacI proteins.
mutable notes: LambdacI usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. By itself, LacI binds downstream from -10. This made the part design very straightforward, since the locations of the operator sites do not overlap at all. In order to complete this part, we simply combined the proper sequences and we had a dually-repressed promoter.
mutable provenance: This part was made by combining BioBrick parts R0010(LacI promoter) and R0051 (LambdacI promoter), along with intermediate sequence between these operator sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101001_sequence/1

BBa_I751501
type: ComponentDefinition
persistent identity: BBa I751501
display id: BBa_I751501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-cI hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER. Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: plux-cI hybrid promoter
sequence annotation: OR2
sequence annotation: -35
sequence annotation: luxR binding
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_I751501_sequence/1

plux-cI hybrid promoter
type: SequenceAnnotation
persistent identity: annotation1955478
display id: annotation1955478
version: 1
top level: BBa_I751501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1
type: Range
persistent identity: range1955478
display id: range1955478
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 66
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1955481
display id: annotation1955481
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1
type: Range
persistent identity: range1955481
display id: range1955481
version: 1
direction: direction/forward
top level: BBa_I751501
start: 26
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955479
display id: annotation1955479
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1
type: Range
persistent identity: range1955479
display id: range1955479
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 25
orientation: inline

luxR binding
type: SequenceAnnotation
persistent identity: annotation1955482
display id: annotation1955482
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1
type: Range
persistent identity: range1955482
display id: range1955482
version: 1
direction: direction/forward
top level: BBa_I751501
start: 1
end: 18
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955480
display id: annotation1955480
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1
type: Range
persistent identity: range1955480
display id: range1955480
version: 1
direction: direction/forward
top level: BBa_I751501
start: 42
end: 47
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1955477
display id: annotation1955477
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1
type: Range
persistent identity: range1955477
display id: range1955477
version: 1
direction: direction/forward
top level: BBa_I751501
start: 48
end: 66
orientation: inline

BBa_K091111
type: ComponentDefinition
persistent identity: BBa K091111
display id: BBa_K091111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacIQ promoter
creator: Robert Cool
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified version of the LacIQ promoter tha should bind LacIQ tighter than the original.
mutable notes: no special considerations
mutable provenance: This is a modified version of the LacIQ promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091111_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1964080
display id: annotation1964080
version: 1
top level: BBa_K091111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091111/annotation1964080/range1964080/1

https://synbiohub.org/public/igem/BBa_K091111/annotation1964080/range1964080/1
type: Range
persistent identity: range1964080
display id: range1964080
version: 1
direction: direction/forward
top level: BBa_K091111
start: 44
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1964079
display id: annotation1964079
version: 1
top level: BBa_K091111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091111/annotation1964079/range1964079/1

https://synbiohub.org/public/igem/BBa_K091111/annotation1964079/range1964079/1
type: Range
persistent identity: range1964079
display id: range1964079
version: 1
direction: direction/forward
top level: BBa_K091111
start: 17
end: 22
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732408
display id: BBa_I732408
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D014O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and RFP) loaded on pSB3C5. For more information please refer to BBa_I732402.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_I732007
sequence annotation: barcode
sequence annotation: BBa_B0010
sequence annotation: stop
sequence annotation: BamHI
sequence annotation: polya
sequence annotation: START
sequence annotation: conserved
sequence annotation: BBa_B0034
sequence annotation: BglII
sequence annotation: stem_loop
sequence annotation: BBa_E1010
sequence annotation: lacZ
sequence annotation: T7 TE
sequence annotation: BBa_I732006
sequence annotation: BBa_B0012
sequence annotation: mrfp1
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732408_sequence/1

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944499
display id: annotation1944499
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944499/range1944499/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944499/range1944499/1
type: Range
persistent identity: range1944499
display id: range1944499
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1944510
display id: annotation1944510
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944510/range1944510/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944510/range1944510/1
type: Range
persistent identity: range1944510
display id: range1944510
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 987
end: 1011
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944511
display id: annotation1944511
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944511/range1944511/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944511/range1944511/1
type: Range
persistent identity: range1944511
display id: range1944511
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1020
end: 1099
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944516
display id: annotation1944516
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944516/range1944516/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944516/range1944516/1
type: Range
persistent identity: range1944516
display id: range1944516
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1141
end: 1141
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944498
display id: annotation1944498
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944498/range1944498/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944498/range1944498/1
type: Range
persistent identity: range1944498
display id: range1944498
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944515
display id: annotation1944515
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944515/range1944515/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944515/range1944515/1
type: Range
persistent identity: range1944515
display id: range1944515
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1135
end: 1148
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944502
display id: annotation1944502
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944502/range1944502/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944502/range1944502/1
type: Range
persistent identity: range1944502
display id: range1944502
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944500
display id: annotation1944500
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944500/range1944500/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944500/range1944500/1
type: Range
persistent identity: range1944500
display id: range1944500
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944501
display id: annotation1944501
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944501/range1944501/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944501/range1944501/1
type: Range
persistent identity: range1944501
display id: range1944501
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944497
display id: annotation1944497
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944497/range1944497/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944497/range1944497/1
type: Range
persistent identity: range1944497
display id: range1944497
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944512
display id: annotation1944512
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944512/range1944512/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944512/range1944512/1
type: Range
persistent identity: range1944512
display id: range1944512
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1031
end: 1074
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1944509
display id: annotation1944509
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944509/range1944509/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944509/range1944509/1
type: Range
persistent identity: range1944509
display id: range1944509
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 986
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944503
display id: annotation1944503
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944503/range1944503/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944503/range1944503/1
type: Range
persistent identity: range1944503
display id: range1944503
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944507
display id: annotation1944507
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944507/range1944507/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944507/range1944507/1
type: Range
persistent identity: range1944507
display id: range1944507
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944514
display id: annotation1944514
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944514/range1944514/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944514/range1944514/1
type: Range
persistent identity: range1944514
display id: range1944514
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1115
end: 1134
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944505
display id: annotation1944505
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944505/range1944505/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944505/range1944505/1
type: Range
persistent identity: range1944505
display id: range1944505
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944513
display id: annotation1944513
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944513/range1944513/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944513/range1944513/1
type: Range
persistent identity: range1944513
display id: range1944513
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1108
end: 1148
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944506
display id: annotation1944506
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944506/range1944506/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944506/range1944506/1
type: Range
persistent identity: range1944506
display id: range1944506
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1944508
display id: annotation1944508
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944508/range1944508/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944508/range1944508/1
type: Range
persistent identity: range1944508
display id: range1944508
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 306
end: 980
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944504
display id: annotation1944504
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944504/range1944504/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944504/range1944504/1
type: Range
persistent identity: range1944504
display id: range1944504
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
direction: direction/forward
top level: LacI
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
direction: direction/forward
top level: LacI
start: 166
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
direction: direction/forward
top level: LacI
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
direction: direction/forward
top level: LacI
start: 137
end: 142
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
direction: direction/forward
top level: LacI
start: 173
end: 173
orientation: inline

Prm+
type: ComponentDefinition
persistent identity: BBa I12001
display id: BBa_I12001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (PRM+)
creator: mcnamara
created: 2004-07-11T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I12001_sequence/1

BBa_K094140
type: ComponentDefinition
persistent identity: BBa K094140
display id: BBa_K094140
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacIq
creator: Liu Chenli
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: mutant promoter of lacI gene, which enables high level of lacI protein expression
mutable notes: no specific considerations
mutable provenance: point mutation made from E. Coli genomic lacI gene promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pLacIq
sequence: https://synbiohub.org/public/igem/BBa_K094140_sequence/1

pLacIq
type: SequenceAnnotation
persistent identity: annotation1981123
display id: annotation1981123
version: 1
top level: BBa_K094140
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K094140/annotation1981123/range1981123/1

https://synbiohub.org/public/igem/BBa_K094140/annotation1981123/range1981123/1
type: Range
persistent identity: range1981123
display id: range1981123
version: 1
direction: direction/forward
top level: BBa_K094140
start: 1
end: 80
orientation: inline

BBa_K086018
type: ComponentDefinition
persistent identity: BBa K086018
display id: BBa_K086018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: LacO and Sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_1
sequence: https://synbiohub.org/public/igem/BBa_K086018_sequence/1

P24_1
type: SequenceAnnotation
persistent identity: annotation1995752
display id: annotation1995752
version: 1
top level: BBa_K086018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086018/annotation1995752/range1995752/1

https://synbiohub.org/public/igem/BBa_K086018/annotation1995752/range1995752/1
type: Range
persistent identity: range1995752
display id: range1995752
version: 1
direction: direction/forward
top level: BBa_K086018
start: 1
end: 55
orientation: inline

LacI:CspC
type: ComponentDefinition
persistent identity: BBa K538204
display id: BBa_K538204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + CspC
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0010
component: BBa_K538004
sequence annotation: BBa_K538004
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K538204_sequence/1

BBa_B0034
type: Component
persistent identity: component2134080
display id: component2134080
version: 1
top level: LacI:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component2134072
display id: component2134072
version: 1
top level: LacI:CspC
access: public
definition: LacI

BBa_K538004
type: Component
persistent identity: component2134086
display id: component2134086
version: 1
top level: LacI:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_K538004/1

BBa_K538004
type: SequenceAnnotation
persistent identity: annotation2134086
display id: annotation2134086
version: 1
top level: LacI:CspC
location: https://synbiohub.org/public/igem/BBa_K538204/annotation2134086/range2134086/1
component: BBa_K538004

https://synbiohub.org/public/igem/BBa_K538204/annotation2134086/range2134086/1
type: Range
persistent identity: range2134086
display id: range2134086
version: 1
direction: direction/reverse
top level: LacI:CspC
start: 227
end: 421
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2134072
display id: annotation2134072
version: 1
top level: LacI:CspC
location: https://synbiohub.org/public/igem/BBa_K538204/annotation2134072/range2134072/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538204/annotation2134072/range2134072/1
type: Range
persistent identity: range2134072
display id: range2134072
version: 1
direction: direction/forward
top level: LacI:CspC
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134080
display id: annotation2134080
version: 1
top level: LacI:CspC
location: https://synbiohub.org/public/igem/BBa_K538204/annotation2134080/range2134080/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538204/annotation2134080/range2134080/1
type: Range
persistent identity: range2134080
display id: range2134080
version: 1
direction: direction/reverse
top level: LacI:CspC
start: 209
end: 220
orientation: inline

BBa_R2201
type: ComponentDefinition
persistent identity: BBa R2201
display id: BBa_R2201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C2006-repressible promoter
creator: Reshma Shetty
created: 2007-08-16T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a promoter designed to be repressed by BBa_C2006: a MBP.Zif268.GCN4 homodimer.
mutable notes: The operator site is positioned to increase the steric hindrance between the repressor and RNAP based on comparing the structures of the repressor bound to DNA and RNAP bound to DNA.
mutable provenance: This sequence is derived from BBa_R2000 and the protein structures of Zif268.GCN4(-2) bound to DNA and RNAP bound to DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: operator
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R2201_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1941585
display id: annotation1941585
version: 1
top level: BBa_R2201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2201/annotation1941585/range1941585/1

https://synbiohub.org/public/igem/BBa_R2201/annotation1941585/range1941585/1
type: Range
persistent identity: range1941585
display id: range1941585
version: 1
direction: direction/forward
top level: BBa_R2201
start: 10
end: 15
orientation: inline

operator
type: SequenceAnnotation
persistent identity: annotation1941586
display id: annotation1941586
version: 1
top level: BBa_R2201
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2201/annotation1941586/range1941586/1

https://synbiohub.org/public/igem/BBa_R2201/annotation1941586/range1941586/1
type: Range
persistent identity: range1941586
display id: range1941586
version: 1
direction: direction/forward
top level: BBa_R2201
start: 14
end: 26
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1941587
display id: annotation1941587
version: 1
top level: BBa_R2201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2201/annotation1941587/range1941587/1

https://synbiohub.org/public/igem/BBa_R2201/annotation1941587/range1941587/1
type: Range
persistent identity: range1941587
display id: range1941587
version: 1
direction: direction/forward
top level: BBa_R2201
start: 33
end: 38
orientation: inline

BBa_K266005
type: ComponentDefinition
persistent identity: BBa K266005
display id: BBa_K266005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI & AI+LuxR --| LasI
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the expresion of LasI enzyme, no terminator
mutable notes: None
mutable provenance: The biobricks BBa_K091146 and BBa_S03154 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K091146
component: BBa_S03154
sequence annotation: BBa_K091146
sequence annotation: BBa_S03154
sequence: https://synbiohub.org/public/igem/BBa_K266005_sequence/1

BBa_K091146
type: Component
persistent identity: component2246677
display id: component2246677
version: 1
top level: BBa_K266005
access: public
definition: BBa_K091146

BBa_S03154
type: Component
persistent identity: component2246684
display id: component2246684
version: 1
top level: BBa_K266005
access: public
definition: https://synbiohub.org/public/igem/BBa_S03154/1

BBa_K091146
type: SequenceAnnotation
persistent identity: annotation2246677
display id: annotation2246677
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
component: BBa_K091146

https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
type: Range
persistent identity: range2246677
display id: range2246677
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 1
end: 126
orientation: inline

BBa_S03154
type: SequenceAnnotation
persistent identity: annotation2246684
display id: annotation2246684
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
component: BBa_S03154

https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
type: Range
persistent identity: range2246684
display id: range2246684
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 135
end: 819
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa R0050
display id: BBa_R0050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (HK022 cI regulated)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The pR regulatory region is a 98 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two operator sites at which HK022 cI protein can bind cooperatively. The -35 and -10 regions from pR are included along with some flanking sequences. The cutoff for the 5' end of the regulatory region was placed just after the pM -10 region which promotes transcription of a gene upstream (thus the pM -10 region is not included). The cutoff for the 3' end of the regulatory region was placed immediately prior to the RBS.
mutable notes: Derived from STHK022N . Literature claims that there is no cross-talk between HK022 cI regulatory region, Lambda cI, 434 cI and P22 cI, but this has not been verified experimentally by the synthetic biology group at MIT.
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: putative
sequence annotation: OR1
sequence annotation: -35
sequence annotation: BBa_R0050
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_R0050_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2014
display id: annotation2014
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1
type: Range
persistent identity: range2014
display id: range2014
version: 1
direction: direction/forward
top level: cI HK022
start: 30
end: 35
orientation: inline

putative
type: SequenceAnnotation
persistent identity: annotation2018
display id: annotation2018
version: 1
top level: cI HK022
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1
type: Range
persistent identity: range2018
display id: range2018
version: 1
direction: direction/forward
top level: cI HK022
start: 43
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2015
display id: annotation2015
version: 1
top level: cI HK022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1
type: Range
persistent identity: range2015
display id: range2015
version: 1
direction: direction/forward
top level: cI HK022
start: 37
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2012
display id: annotation2012
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1
type: Range
persistent identity: range2012
display id: range2012
version: 1
direction: direction/forward
top level: cI HK022
start: 8
end: 13
orientation: inline

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation7066
display id: annotation7066
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1

https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1
type: Range
persistent identity: range7066
display id: range7066
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 55
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2013
display id: annotation2013
version: 1
top level: cI HK022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1
type: Range
persistent identity: range2013
display id: range2013
version: 1
direction: direction/forward
top level: cI HK022
start: 13
end: 27
orientation: inline

BBa_K389052
type: ComponentDefinition
persistent identity: BBa K389052
display id: BBa_K389052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tightly regulated lacO + mRFP
creator: Jonas Aretz
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the tightly regulated lac operon BBa_K389050 with a mRFP gene as readout.
mutable notes: lacIq integrated in promoter so good regulation, mRFP as readout
mutable provenance: partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E1010
component: BBa_K389050
component: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_K389050
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389052_sequence/1

BBa_E1010
type: Component
persistent identity: component2273045
display id: component2273045
version: 1
top level: BBa_K389052
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K389050
type: Component
persistent identity: component2273040
display id: component2273040
version: 1
top level: BBa_K389052
access: public
definition: BBa_K389050

BBa_B0034
type: Component
persistent identity: component2273042
display id: component2273042
version: 1
top level: BBa_K389052
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2273045
display id: annotation2273045
version: 1
top level: BBa_K389052
location: https://synbiohub.org/public/igem/BBa_K389052/annotation2273045/range2273045/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389052/annotation2273045/range2273045/1
type: Range
persistent identity: range2273045
display id: range2273045
version: 1
direction: direction/reverse
top level: BBa_K389052
start: 1627
end: 2332
orientation: inline

BBa_K389050
type: SequenceAnnotation
persistent identity: annotation2273040
display id: annotation2273040
version: 1
top level: BBa_K389052
location: https://synbiohub.org/public/igem/BBa_K389052/annotation2273040/range2273040/1
component: BBa_K389050

https://synbiohub.org/public/igem/BBa_K389052/annotation2273040/range2273040/1
type: Range
persistent identity: range2273040
display id: range2273040
version: 1
direction: direction/reverse
top level: BBa_K389052
start: 1
end: 1600
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273042
display id: annotation2273042
version: 1
top level: BBa_K389052
location: https://synbiohub.org/public/igem/BBa_K389052/annotation2273042/range2273042/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389052/annotation2273042/range2273042/1
type: Range
persistent identity: range2273042
display id: range2273042
version: 1
direction: direction/reverse
top level: BBa_K389052
start: 1609
end: 1620
orientation: inline

BBa_K091105
type: ComponentDefinition
persistent identity: BBa K091105
display id: BBa_K091105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet/Mnt Hybrid Promoter
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to TetR. The promoter should be repressed by Mnt repressor. It should also be repressed by TetR, and in the absence of Mnt repressor, should be induced by aTc.
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of TetR binding sites between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by TetR. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tccctatcagtgata, and 2) truncate the second half of ptet -10 and mutate -35 so that it function to bind tetR (replace ttgaca with actgta), but is not a promoter, and 3) place the tetR1 and tetR2 binding sites 3??? to truncated mnt -10 sequence.
mutable provenance: This part comes from the Mnt promoter and the TetR promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mnt
sequence annotation: -10
sequence annotation: tetR2
sequence annotation: tetR1
sequence: https://synbiohub.org/public/igem/BBa_K091105_sequence/1

Mnt
type: SequenceAnnotation
persistent identity: annotation1963742
display id: annotation1963742
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1
type: Range
persistent identity: range1963742
display id: range1963742
version: 1
direction: direction/forward
top level: BBa_K091105
start: 1
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963739
display id: annotation1963739
version: 1
top level: BBa_K091105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1
type: Range
persistent identity: range1963739
display id: range1963739
version: 1
direction: direction/forward
top level: BBa_K091105
start: 47
end: 52
orientation: inline

tetR2
type: SequenceAnnotation
persistent identity: annotation1963741
display id: annotation1963741
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1
type: Range
persistent identity: range1963741
display id: range1963741
version: 1
direction: direction/forward
top level: BBa_K091105
start: 78
end: 98
orientation: inline

tetR1
type: SequenceAnnotation
persistent identity: annotation1963740
display id: annotation1963740
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1
type: Range
persistent identity: range1963740
display id: range1963740
version: 1
direction: direction/forward
top level: BBa_K091105
start: 53
end: 71
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732448
display id: BBa_I732448
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732448_sequence/1

BBa_I756017
type: ComponentDefinition
persistent identity: BBa I756017
display id: BBa_I756017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: U6 promoter with tet operators
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U6 promoter with tet operators
mutable notes: U6 promoter with tet operators
mutable provenance: U6 promoter with tet operators
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756017_sequence/1

BBa_K112318
type: ComponentDefinition
persistent identity: BBa K112318
display id: BBa_K112318
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< bolA promoter>} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bolA promoter is a promoter where levels of expression are inversely proportional to the growth rate. To read more, go to following link [http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=402084&blobtype=pdf] This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0017/al0018 on MG1655 (467bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112318 ----------------------------------------------- al0017 Forward Biobricking of bolA promoter CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc al0018 Reverse Biobricking of bolA promoter CGttaGGATCCcttctatccgctcacgtatc
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112318_sequence/1

BBa_K091157
type: ComponentDefinition
persistent identity: BBa K091157
display id: BBa_K091157
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/Las Hybrid Promoter
creator: Erin Feeney
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: K091157 is a modified version of the pLux' promoter (BBa_K091156) containing the addition of a LasR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of AI-1 and LuxR and repressed in the presence of PAI-1 and LasR.
mutable notes: In order to fit the LasR binding site between the -35 and -10 regions, the last base of the Las Box was cropped and the -35 region was modified to overlap with the first two bases of the Las Box.
mutable provenance: The LasR binding site is from the region upstream of the LasI gene in Pseudomonas aeruginosa. The rest of the promoter is the same as BBa_K091156.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR+AI-1
sequence annotation: -35 Region
sequence annotation: -10 Region
sequence annotation: LasR + PAI-1
sequence: https://synbiohub.org/public/igem/BBa_K091157_sequence/1

LuxR+AI-1
type: SequenceAnnotation
persistent identity: annotation1968426
display id: annotation1968426
version: 1
top level: BBa_K091157
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968426/range1968426/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968426/range1968426/1
type: Range
persistent identity: range1968426
display id: range1968426
version: 1
direction: direction/forward
top level: BBa_K091157
start: 1
end: 20
orientation: inline

-35 Region
type: SequenceAnnotation
persistent identity: annotation1968423
display id: annotation1968423
version: 1
top level: BBa_K091157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968423/range1968423/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968423/range1968423/1
type: Range
persistent identity: range1968423
display id: range1968423
version: 1
direction: direction/forward
top level: BBa_K091157
start: 20
end: 25
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation1968424
display id: annotation1968424
version: 1
top level: BBa_K091157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968424/range1968424/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968424/range1968424/1
type: Range
persistent identity: range1968424
display id: range1968424
version: 1
direction: direction/forward
top level: BBa_K091157
start: 42
end: 47
orientation: inline

LasR + PAI-1
type: SequenceAnnotation
persistent identity: annotation1968425
display id: annotation1968425
version: 1
top level: BBa_K091157
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968425/range1968425/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968425/range1968425/1
type: Range
persistent identity: range1968425
display id: range1968425
version: 1
direction: direction/forward
top level: BBa_K091157
start: 24
end: 42
orientation: inline

BBa_K389051
type: ComponentDefinition
persistent identity: BBa K389051
display id: BBa_K389051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tightly regulated lacO + luc
creator: Jonas Aretz
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the tightly regulated lac operon K389050 with a luciferase gene as readout.
mutable notes: lacIq integrated in promoter so good regulation, luciferase as sensitive readout
mutable provenance: partsregistry, pGL4.10luc2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K389050
component: BBa_K389004
sequence annotation: BBa_K389004
sequence annotation: BBa_K389050
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389051_sequence/1

BBa_B0034
type: Component
persistent identity: component2273070
display id: component2273070
version: 1
top level: BBa_K389051
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389050
type: Component
persistent identity: component2273068
display id: component2273068
version: 1
top level: BBa_K389051
access: public
definition: BBa_K389050

BBa_K389004
type: Component
persistent identity: component2273073
display id: component2273073
version: 1
top level: BBa_K389051
access: public
definition: https://synbiohub.org/public/igem/BBa_K389004/1

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2273073
display id: annotation2273073
version: 1
top level: BBa_K389051
location: https://synbiohub.org/public/igem/BBa_K389051/annotation2273073/range2273073/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389051/annotation2273073/range2273073/1
type: Range
persistent identity: range2273073
display id: range2273073
version: 1
direction: direction/reverse
top level: BBa_K389051
start: 1627
end: 3282
orientation: inline

BBa_K389050
type: SequenceAnnotation
persistent identity: annotation2273068
display id: annotation2273068
version: 1
top level: BBa_K389051
location: https://synbiohub.org/public/igem/BBa_K389051/annotation2273068/range2273068/1
component: BBa_K389050

https://synbiohub.org/public/igem/BBa_K389051/annotation2273068/range2273068/1
type: Range
persistent identity: range2273068
display id: range2273068
version: 1
direction: direction/reverse
top level: BBa_K389051
start: 1
end: 1600
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273070
display id: annotation2273070
version: 1
top level: BBa_K389051
location: https://synbiohub.org/public/igem/BBa_K389051/annotation2273070/range2273070/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389051/annotation2273070/range2273070/1
type: Range
persistent identity: range2273070
display id: range2273070
version: 1
direction: direction/reverse
top level: BBa_K389051
start: 1609
end: 1620
orientation: inline

MicF
type: ComponentDefinition
persistent identity: BBa K1766000
display id: BBa_K1766000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MicF non-coding regulatory RNA
creator: Sarah Wideman
created: 2015-08-25T11:00:00Z
modified: 2015-09-16T11:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2188_
m user id: 25546
owner id: 25546
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5??? untranslated region of ompF mRNA, to prevent translation and promote degradation. MicF can be used together with BBa_K176601 to regulate protein expression.
mutable notes: The transcribed MicF only consists of the last 93 nt of BBa_K1766000. The preceding region was added in consideration of the genomic sequence.
mutable provenance: BBa_K1766000 is based on the micF gene found in Escherichia coli. The BioBrick??? was created from a gBlocks?? gene fragment synthesized by Integrated DNA Technologies.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: MicF
sequence: https://synbiohub.org/public/igem/BBa_K1766000_sequence/1

MicF
type: SequenceAnnotation
persistent identity: annotation2438244
display id: annotation2438244
version: 1
top level: MicF
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1766000/annotation2438244/range2438244/1

https://synbiohub.org/public/igem/BBa_K1766000/annotation2438244/range2438244/1
type: Range
persistent identity: range2438244
display id: range2438244
version: 1
direction: direction/forward
top level: MicF
start: 147
end: 239
orientation: inline

pLac(B4)
type: ComponentDefinition
persistent identity: BBa K137125
display id: BBa_K137125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI-repressed promoter B4
creator: Josh Michener
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 253
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LacI-repressible promoter B4 developed by Sidney Cox (http://www.nature.com/msb/journal/v3/n1/full/msb4100187.html). Showed a regulatory range (ON/OFF) of 2.5x10^4.
mutable provenance: Annealed from oligos.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137125_sequence/1

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732427
display id: BBa_I732427
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732427_sequence/1

BBa_K259005
type: ComponentDefinition
persistent identity: BBa K259005
display id: BBa_K259005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Rheostat Promoter
creator: Petros Mina
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the expression of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. This composite part does not have an LVA degradation tag at its end, hence can be used with all prokaryotic assemblies.
mutable notes: The part was designed and assembled from the iGEM2009 kit with standard RFC10 assembly.
mutable provenance: The AraC promoter is found naturally in E.Coli at 1.52 min/centisome in the clockwise orientation. More information can be found at [http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0081
component: BBa_R0080
sequence annotation: BBa_R0080
sequence annotation: BBa_R0081
sequence: https://synbiohub.org/public/igem/BBa_K259005_sequence/1

BBa_R0081
type: Component
persistent identity: component2018287
display id: component2018287
version: 1
top level: BBa_K259005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2018294
display id: component2018294
version: 1
top level: BBa_K259005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2018294
display id: annotation2018294
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018294/range2018294/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259005/annotation2018294/range2018294/1
type: Range
persistent identity: range2018294
display id: range2018294
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 192
end: 340
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2018287
display id: annotation2018287
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018287/range2018287/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259005/annotation2018287/range2018287/1
type: Range
persistent identity: range2018287
display id: range2018287
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 1
end: 183
orientation: inline

BBa_K1165004
type: ComponentDefinition
persistent identity: BBa K1165004
display id: BBa_K1165004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 1 of XOR Gate 2
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Target Binding Arms
sequence annotation: Synthetic Extended Arms
sequence annotation: Target-Binding Arms
sequence annotation: TAA Bump
sequence annotation: Catalytic Core
sequence: https://synbiohub.org/public/igem/BBa_K1165004_sequence/1

Target Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365277
display id: annotation2365277
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365277/range2365277/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365277/range2365277/1
type: Range
persistent identity: range2365277
display id: range2365277
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 39
end: 43
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365271
display id: annotation2365271
version: 1
top level: BBa_K1165004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365271/range2365271/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365271/range2365271/1
type: Range
persistent identity: range2365271
display id: range2365271
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 1
end: 30
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365280
display id: annotation2365280
version: 1
top level: BBa_K1165004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365280/range2365280/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365280/range2365280/1
type: Range
persistent identity: range2365280
display id: range2365280
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 82
end: 111
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365272
display id: annotation2365272
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365272/range2365272/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365272/range2365272/1
type: Range
persistent identity: range2365272
display id: range2365272
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 33
end: 35
orientation: inline

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2365273
display id: annotation2365273
version: 1
top level: BBa_K1165004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365273/range2365273/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365273/range2365273/1
type: Range
persistent identity: range2365273
display id: range2365273
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 36
end: 38
orientation: inline

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2365278
display id: annotation2365278
version: 1
top level: BBa_K1165004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365278/range2365278/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365278/range2365278/1
type: Range
persistent identity: range2365278
display id: range2365278
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 44
end: 69
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365279
display id: annotation2365279
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365279/range2365279/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365279/range2365279/1
type: Range
persistent identity: range2365279
display id: range2365279
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 70
end: 81
orientation: inline

BBa_K165043
type: ComponentDefinition
persistent identity: BBa K165043
display id: BBa_K165043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + MET25 constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165000
component: BBa_K165011
sequence annotation: BBa_K165011
sequence annotation: BBa_K165000
sequence: https://synbiohub.org/public/igem/BBa_K165043_sequence/1

BBa_K165000
type: Component
persistent identity: component1996570
display id: component1996570
version: 1
top level: BBa_K165043
access: public
definition: BBa_K165000

BBa_K165011
type: Component
persistent identity: component1996569
display id: component1996569
version: 1
top level: BBa_K165043
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996569
display id: annotation1996569
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
type: Range
persistent identity: range1996569
display id: range1996569
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 1
end: 46
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996570
display id: annotation1996570
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
type: Range
persistent identity: range1996570
display id: range1996570
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 55
end: 441
orientation: inline

Pnot(O1wt1
type: ComponentDefinition
persistent identity: BBa I732200
display id: BBa_I732200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O1wt1)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732200_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732411
display id: BBa_I732411
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D050O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: consensus elements
sequence annotation: O2
sequence: https://synbiohub.org/public/igem/BBa_I732411_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation1944563
display id: annotation1944563
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944563/range1944563/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944563/range1944563/1
type: Range
persistent identity: range1944563
display id: range1944563
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944561
display id: annotation1944561
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944561/range1944561/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944561/range1944561/1
type: Range
persistent identity: range1944561
display id: range1944561
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 61
end: 137
orientation: inline

O2
type: SequenceAnnotation
persistent identity: annotation1944562
display id: annotation1944562
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944562/range1944562/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944562/range1944562/1
type: Range
persistent identity: range1944562
display id: range1944562
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 146
end: 165
orientation: inline

BBa_R2110
type: ComponentDefinition
persistent identity: BBa R2110
display id: BBa_R2110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was designed rationally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: transcription start site
sequence annotation: -35 site
sequence annotation: -10 site
sequence annotation: C2003 operator site
sequence: https://synbiohub.org/public/igem/BBa_R2110_sequence/1

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903918
display id: annotation1903918
version: 1
top level: BBa_R2110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903918/range1903918/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903918/range1903918/1
type: Range
persistent identity: range1903918
display id: range1903918
version: 1
direction: direction/forward
top level: BBa_R2110
start: 53
end: 53
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1903916
display id: annotation1903916
version: 1
top level: BBa_R2110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903916/range1903916/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903916/range1903916/1
type: Range
persistent identity: range1903916
display id: range1903916
version: 1
direction: direction/forward
top level: BBa_R2110
start: 17
end: 22
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903917
display id: annotation1903917
version: 1
top level: BBa_R2110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903917/range1903917/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903917/range1903917/1
type: Range
persistent identity: range1903917
display id: range1903917
version: 1
direction: direction/forward
top level: BBa_R2110
start: 40
end: 45
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1903919
display id: annotation1903919
version: 1
top level: BBa_R2110
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903919/range1903919/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903919/range1903919/1
type: Range
persistent identity: range1903919
display id: range1903919
version: 1
direction: direction/forward
top level: BBa_R2110
start: 33
end: 44
orientation: inline

NAND2717
type: ComponentDefinition
persistent identity: BBa I732302
display id: BBa_I732302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O27D001O17)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O22
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732302_sequence/1

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732447
display id: BBa_I732447
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732447_sequence/1

cI TP901
type: ComponentDefinition
persistent identity: BBa R0075
display id: BBa_R0075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (TP901 cI regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: cI repressor from phage TP901 infecting L. lactis
mutable notes: operator sites: pR and pL
mutable provenance: TP901-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: cI tp901 OR
sequence annotation: start
sequence annotation: cI tp901 OL
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0075_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation319985
display id: annotation319985
version: 1
top level: cI TP901
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319985/range319985/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319985/range319985/1
type: Range
persistent identity: range319985
display id: range319985
version: 1
direction: direction/forward
top level: cI TP901
start: 76
end: 82
orientation: inline

cI tp901 OR
type: SequenceAnnotation
persistent identity: annotation302714
display id: annotation302714
version: 1
top level: cI TP901
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0075/annotation302714/range302714/1

https://synbiohub.org/public/igem/BBa_R0075/annotation302714/range302714/1
type: Range
persistent identity: range302714
display id: range302714
version: 1
direction: direction/forward
top level: cI TP901
start: 19
end: 57
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation320591
display id: annotation320591
version: 1
top level: cI TP901
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0075/annotation320591/range320591/1

https://synbiohub.org/public/igem/BBa_R0075/annotation320591/range320591/1
type: Range
persistent identity: range320591
display id: range320591
version: 1
direction: direction/forward
top level: cI TP901
start: 89
end: 89
orientation: inline

cI tp901 OL
type: SequenceAnnotation
persistent identity: annotation319984
display id: annotation319984
version: 1
top level: cI TP901
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319984/range319984/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319984/range319984/1
type: Range
persistent identity: range319984
display id: range319984
version: 1
direction: direction/forward
top level: cI TP901
start: 85
end: 115
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319983
display id: annotation319983
version: 1
top level: cI TP901
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319983/range319983/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319983/range319983/1
type: Range
persistent identity: range319983
display id: range319983
version: 1
direction: direction/forward
top level: cI TP901
start: 53
end: 59
orientation: inline

BBa_K292002
type: ComponentDefinition
persistent identity: BBa K292002
display id: BBa_K292002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac (LacI regulated) + Strong RBS
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: This part contains the pLac promoter and a ribosome binding site (BBa_R0010 + BBa_B0030). The Lac promoter is constitutively activated, but it can be repressed by the Lac I repressor from E.coli. This promoter is really useful to control a gene expression by inactivating it with LacI repressor. When LacI is absent the gene should be expressed, and in the presence of LacI, pLac is inhibited. So this part is really suitable for gene expression control by inactivation of pLac with LacI. We can use this part to control a failed feedback mechanism which induces the Lambda phage lytic cycle. In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside). This part already contains a strong RBS to begin directly a gene transcription.
mutable notes: N/A
mutable provenance: Part:BBa_R0010 Part:BBa_B0030, Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0030
component: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K292002_sequence/1

BBa_B0030
type: Component
persistent identity: component2054553
display id: component2054553
version: 1
top level: BBa_K292002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0010
type: Component
persistent identity: component2054545
display id: component2054545
version: 1
top level: BBa_K292002
access: public
definition: LacI

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2054553
display id: annotation2054553
version: 1
top level: BBa_K292002
location: https://synbiohub.org/public/igem/BBa_K292002/annotation2054553/range2054553/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K292002/annotation2054553/range2054553/1
type: Range
persistent identity: range2054553
display id: range2054553
version: 1
direction: direction/forward
top level: BBa_K292002
start: 209
end: 223
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2054545
display id: annotation2054545
version: 1
top level: BBa_K292002
location: https://synbiohub.org/public/igem/BBa_K292002/annotation2054545/range2054545/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K292002/annotation2054545/range2054545/1
type: Range
persistent identity: range2054545
display id: range2054545
version: 1
direction: direction/forward
top level: BBa_K292002
start: 1
end: 200
orientation: inline

BBa_J64981
type: ComponentDefinition
persistent identity: BBa J64981
display id: BBa_J64981
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR-P strong binding, regulatory region for Team Challenge03-2007
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Contains the F1 consensus site from the OmpF regulator. Contains a LacI repressor consensus binding site between the -35 and -10 portions of a T7 core promoter region, and a TetR consensus binding site upstream of the -35 site.
mutable notes: The weaker binding repressor binds upstream of the core promoter (upstream of -35), while the stronger binds inbetween the -35 and -10.
mutable provenance: Information for creation of this construct came from the following sorces: Yoshida et al. JBC 2006 Frank et al. JMB 1997 Rong et al. PNAS 1998 Merabet et al. Biochemistry 1995 Su et al. JBC 1998 Kedracka-Krok et al. JPC 1999 Kleinschmidt et al. Biochemistry 1988 Butler et al. JBC 1982 Jorgensen et al. JBC 1991 Hillen et al. JMB 1984 Head et al. JMB 1998
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: T7 RNA Polymerase Recognition
sequence annotation: Strong OmpR
sequence annotation: LacI Consensus
sequence annotation: -10
sequence annotation: TetR Consensus
sequence: https://synbiohub.org/public/igem/BBa_J64981_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1921602
display id: annotation1921602
version: 1
top level: BBa_J64981
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921602/range1921602/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921602/range1921602/1
type: Range
persistent identity: range1921602
display id: range1921602
version: 1
direction: direction/forward
top level: BBa_J64981
start: 40
end: 45
orientation: inline

T7 RNA Polymerase Recognition
type: SequenceAnnotation
persistent identity: annotation1921600
display id: annotation1921600
version: 1
top level: BBa_J64981
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921600/range1921600/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921600/range1921600/1
type: Range
persistent identity: range1921600
display id: range1921600
version: 1
direction: direction/forward
top level: BBa_J64981
start: 40
end: 82
orientation: inline

Strong OmpR
type: SequenceAnnotation
persistent identity: annotation1921598
display id: annotation1921598
version: 1
top level: BBa_J64981
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921598/range1921598/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921598/range1921598/1
type: Range
persistent identity: range1921598
display id: range1921598
version: 1
direction: direction/forward
top level: BBa_J64981
start: 1
end: 20
orientation: inline

LacI Consensus
type: SequenceAnnotation
persistent identity: annotation1921601
display id: annotation1921601
version: 1
top level: BBa_J64981
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921601/range1921601/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921601/range1921601/1
type: Range
persistent identity: range1921601
display id: range1921601
version: 1
direction: direction/forward
top level: BBa_J64981
start: 46
end: 65
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921603
display id: annotation1921603
version: 1
top level: BBa_J64981
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921603/range1921603/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921603/range1921603/1
type: Range
persistent identity: range1921603
display id: range1921603
version: 1
direction: direction/forward
top level: BBa_J64981
start: 66
end: 70
orientation: inline

TetR Consensus
type: SequenceAnnotation
persistent identity: annotation1921599
display id: annotation1921599
version: 1
top level: BBa_J64981
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921599/range1921599/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921599/range1921599/1
type: Range
persistent identity: range1921599
display id: range1921599
version: 1
direction: direction/forward
top level: BBa_J64981
start: 21
end: 39
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732406
display id: BBa_I732406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732403.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: conserved
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: GFP protein
sequence annotation: stop
sequence annotation: BBa_I732006
sequence annotation: BBa_B0034
sequence annotation: BglII
sequence annotation: BBa_I732007
sequence annotation: polya
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence annotation: T7 TE
sequence annotation: START
sequence annotation: BamHI
sequence annotation: lacZ
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732406_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation1944469
display id: annotation1944469
version: 1
top level: U037O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944469/range1944469/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944469/range1944469/1
type: Range
persistent identity: range1944469
display id: range1944469
version: 1
direction: direction/forward
top level: U037O11+D0
start: 292
end: 295
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944475
display id: annotation1944475
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944475/range1944475/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944475/range1944475/1
type: Range
persistent identity: range1944475
display id: range1944475
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1122
end: 1162
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944463
display id: annotation1944463
version: 1
top level: U037O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944463/range1944463/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944463/range1944463/1
type: Range
persistent identity: range1944463
display id: range1944463
version: 1
direction: direction/forward
top level: U037O11+D0
start: 32
end: 35
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1944472
display id: annotation1944472
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944472/range1944472/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944472/range1944472/1
type: Range
persistent identity: range1944472
display id: range1944472
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 306
end: 1025
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation1944471
display id: annotation1944471
version: 1
top level: U037O11+D0
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944471/range1944471/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944471/range1944471/1
type: Range
persistent identity: range1944471
display id: range1944471
version: 1
direction: direction/forward
top level: U037O11+D0
start: 306
end: 1025
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944478
display id: annotation1944478
version: 1
top level: U037O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944478/range1944478/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944478/range1944478/1
type: Range
persistent identity: range1944478
display id: range1944478
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1155
end: 1155
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944468
display id: annotation1944468
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944468/range1944468/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944468/range1944468/1
type: Range
persistent identity: range1944468
display id: range1944468
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944464
display id: annotation1944464
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944464/range1944464/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944464/range1944464/1
type: Range
persistent identity: range1944464
display id: range1944464
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 28
end: 39
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944460
display id: annotation1944460
version: 1
top level: U037O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944460/range1944460/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944460/range1944460/1
type: Range
persistent identity: range1944460
display id: range1944460
version: 1
direction: direction/forward
top level: U037O11+D0
start: 3
end: 8
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944462
display id: annotation1944462
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944462/range1944462/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944462/range1944462/1
type: Range
persistent identity: range1944462
display id: range1944462
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 1
end: 19
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944477
display id: annotation1944477
version: 1
top level: U037O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944477/range1944477/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944477/range1944477/1
type: Range
persistent identity: range1944477
display id: range1944477
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1149
end: 1162
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944474
display id: annotation1944474
version: 1
top level: U037O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944474/range1944474/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944474/range1944474/1
type: Range
persistent identity: range1944474
display id: range1944474
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1045
end: 1088
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944473
display id: annotation1944473
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944473/range1944473/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944473/range1944473/1
type: Range
persistent identity: range1944473
display id: range1944473
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1034
end: 1113
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944476
display id: annotation1944476
version: 1
top level: U037O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944476/range1944476/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944476/range1944476/1
type: Range
persistent identity: range1944476
display id: range1944476
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1129
end: 1148
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944465
display id: annotation1944465
version: 1
top level: U037O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944465/range1944465/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944465/range1944465/1
type: Range
persistent identity: range1944465
display id: range1944465
version: 1
direction: direction/forward
top level: U037O11+D0
start: 46
end: 48
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944470
display id: annotation1944470
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944470/range1944470/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944470/range1944470/1
type: Range
persistent identity: range1944470
display id: range1944470
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 288
end: 299
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944461
display id: annotation1944461
version: 1
top level: U037O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944461/range1944461/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944461/range1944461/1
type: Range
persistent identity: range1944461
display id: range1944461
version: 1
direction: direction/forward
top level: U037O11+D0
start: 12
end: 17
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944466
display id: annotation1944466
version: 1
top level: U037O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944466/range1944466/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944466/range1944466/1
type: Range
persistent identity: range1944466
display id: range1944466
version: 1
direction: direction/forward
top level: U037O11+D0
start: 46
end: 279
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944467
display id: annotation1944467
version: 1
top level: U037O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944467/range1944467/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944467/range1944467/1
type: Range
persistent identity: range1944467
display id: range1944467
version: 1
direction: direction/forward
top level: U037O11+D0
start: 274
end: 279
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732421
display id: BBa_I732421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D026O22)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Our first NOR logical gate
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732421_sequence/1

BBa_K116500
type: ComponentDefinition
persistent identity: BBa K116500
display id: BBa_K116500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpF promoter that is activated or repressesed by OmpR according to osmolarity.
creator: Chun-Ju Yang
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC. Reference: K. Mattison, R. Oropeza, N. Byers, L. J. Kenney, J Mol Biol 315, 497 (Jan 25, 2002).
mutable notes: The osmolarity range of OmpF sensing still have to be confirmed
mutable provenance: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OmpR binding
sequence: https://synbiohub.org/public/igem/BBa_K116500_sequence/1

OmpR binding
type: SequenceAnnotation
persistent identity: annotation1994040
display id: annotation1994040
version: 1
top level: BBa_K116500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1

https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1
type: Range
persistent identity: range1994040
display id: range1994040
version: 1
direction: direction/forward
top level: BBa_K116500
start: 27
end: 88
orientation: inline

BBa_K227007
type: ComponentDefinition
persistent identity: BBa K227007
display id: BBa_K227007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: puc promoter
creator: WashU iGEM 2009
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _326_
m user id: 0
owner id: 4645
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The puc promoter is the primary promoter for the puc operon. The puc operon consists of puc A and B coding regions responsible for the LH II antenna complex essential for light harvesting in Rhodobacter sphaeroides. The puc promoter is ultimately controlled by oxygen tension, and is a very strong promoter under low oxygen concentration.
mutable notes: -
mutable provenance: PCR applified from plasmid pRKCBC3 provided by Dr. Neil Hunter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PpsR
sequence: https://synbiohub.org/public/igem/BBa_K227007_sequence/1

PpsR
type: SequenceAnnotation
persistent identity: annotation2057660
display id: annotation2057660
version: 1
top level: BBa_K227007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K227007/annotation2057660/range2057660/1

https://synbiohub.org/public/igem/BBa_K227007/annotation2057660/range2057660/1
type: Range
persistent identity: range2057660
display id: range2057660
version: 1
direction: direction/forward
top level: BBa_K227007
start: 482
end: 499
orientation: inline

PpsR
type: SequenceAnnotation
persistent identity: annotation2057661
display id: annotation2057661
version: 1
top level: BBa_K227007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K227007/annotation2057661/range2057661/1

https://synbiohub.org/public/igem/BBa_K227007/annotation2057661/range2057661/1
type: Range
persistent identity: range2057661
display id: range2057661
version: 1
direction: direction/forward
top level: BBa_K227007
start: 507
end: 524
orientation: inline

BBa_K1520005
type: ComponentDefinition
persistent identity: BBa K1520005
display id: BBa_K1520005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plac-rbs-rfp-Ter It produce rfp when there is enough IPTG or less lacI
mutable notes: None
mutable provenance: Plac, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1520029
component: BBa_R0010
component: BBa_K1520500
sequence annotation: BBa_K1520500
sequence annotation: BBa_R0010
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520005_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384907
display id: component2384907
version: 1
top level: BBa_K1520005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0010
type: Component
persistent identity: component2384899
display id: component2384899
version: 1
top level: BBa_K1520005
access: public
definition: LacI

BBa_K1520500
type: Component
persistent identity: component2384906
display id: component2384906
version: 1
top level: BBa_K1520005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384906
display id: annotation2384906
version: 1
top level: BBa_K1520005
location: https://synbiohub.org/public/igem/BBa_K1520005/annotation2384906/range2384906/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520005/annotation2384906/range2384906/1
type: Range
persistent identity: range2384906
display id: range2384906
version: 1
direction: direction/reverse
top level: BBa_K1520005
start: 209
end: 940
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384899
display id: annotation2384899
version: 1
top level: BBa_K1520005
location: https://synbiohub.org/public/igem/BBa_K1520005/annotation2384899/range2384899/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1520005/annotation2384899/range2384899/1
type: Range
persistent identity: range2384899
display id: range2384899
version: 1
direction: direction/forward
top level: BBa_K1520005
start: 1
end: 200
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384907
display id: annotation2384907
version: 1
top level: BBa_K1520005
location: https://synbiohub.org/public/igem/BBa_K1520005/annotation2384907/range2384907/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520005/annotation2384907/range2384907/1
type: Range
persistent identity: range2384907
display id: range2384907
version: 1
direction: direction/reverse
top level: BBa_K1520005
start: 949
end: 1077
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732428
display id: BBa_I732428
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732428_sequence/1

cI HK022
type: ComponentDefinition
persistent identity: BBa R1050
display id: BBa_R1050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (HK022 cI regulated)
creator: Reshma Shetty, Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0050 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The pR regulatory region is a 98 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two operator sites at which HK022 cI protein can bind cooperatively. The -35 and -10 regions from pR are included along with some flanking sequences. The cutoff for the 5' end of the regulatory region was placed just after the pM -10 region which promotes transcription of a gene upstream (thus the pM -10 region is not included). The cutoff for the 3' end of the regulatory region was placed immediately prior to the RBS.
mutable notes: Derived from STHK022N . Modified to comply with BBa_S0001: TTGACA-17N-GATACT Literature claims that there is no cross-talk between HK022 cI regulatory region, Lambda cI, 434 cI and P22 cI, but this has not been verified experimentally by the synthetic biology group at MIT.
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: BBa_R1050
sequence annotation: -35
sequence annotation: putative
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1050_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation2066
display id: annotation2066
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2066/range2066/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2066/range2066/1
type: Range
persistent identity: range2066
display id: range2066
version: 1
direction: direction/forward
top level: cI HK022
start: 14
end: 28
orientation: inline

BBa_R1050
type: SequenceAnnotation
persistent identity: annotation7073
display id: annotation7073
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1050/annotation7073/range7073/1

https://synbiohub.org/public/igem/BBa_R1050/annotation7073/range7073/1
type: Range
persistent identity: range7073
display id: range7073
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 56
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2065
display id: annotation2065
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2065/range2065/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2065/range2065/1
type: Range
persistent identity: range2065
display id: range2065
version: 1
direction: direction/forward
top level: cI HK022
start: 8
end: 14
orientation: inline

putative
type: SequenceAnnotation
persistent identity: annotation2070
display id: annotation2070
version: 1
top level: cI HK022
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2070/range2070/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2070/range2070/1
type: Range
persistent identity: range2070
display id: range2070
version: 1
direction: direction/forward
top level: cI HK022
start: 44
end: 44
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2067
display id: annotation2067
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2067/range2067/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2067/range2067/1
type: Range
persistent identity: range2067
display id: range2067
version: 1
direction: direction/forward
top level: cI HK022
start: 38
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2071
display id: annotation2071
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2071/range2071/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2071/range2071/1
type: Range
persistent identity: range2071
display id: range2071
version: 1
direction: direction/forward
top level: cI HK022
start: 31
end: 36
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732402
display id: BBa_I732402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D062NUL)
creator: Zhan Jian
created: 2007-09-20T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Might be later edited.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: O1
sequence annotation: consensus elements
sequence: https://synbiohub.org/public/igem/BBa_I732402_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation1944541
display id: annotation1944541
version: 1
top level: U085O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944541/range1944541/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944541/range1944541/1
type: Range
persistent identity: range1944541
display id: range1944541
version: 1
direction: direction/forward
top level: U085O11+D0
start: 105
end: 105
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1944540
display id: annotation1944540
version: 1
top level: U085O11+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944540/range1944540/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944540/range1944540/1
type: Range
persistent identity: range1944540
display id: range1944540
version: 1
direction: direction/forward
top level: U085O11+D0
start: 1
end: 20
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944539
display id: annotation1944539
version: 1
top level: U085O11+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944539/range1944539/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944539/range1944539/1
type: Range
persistent identity: range1944539
display id: range1944539
version: 1
direction: direction/forward
top level: U085O11+D0
start: 69
end: 105
orientation: inline

BBa_K266011
type: ComponentDefinition
persistent identity: BBa K266011
display id: BBa_K266011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Inverter and strong RBS
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 5491
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: POPS regulated and Lac inverter system with strong RBS
mutable notes: None
mutable provenance: The biobricks BBa_Q04121 and BBa_B0034 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0034
component: BBa_Q04121
sequence annotation: BBa_Q04121
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K266011_sequence/1

BBa_B0034
type: Component
persistent identity: component2262985
display id: component2262985
version: 1
top level: BBa_K266011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_Q04121
type: Component
persistent identity: component2262983
display id: component2262983
version: 1
top level: BBa_K266011
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2262983
display id: annotation2262983
version: 1
top level: BBa_K266011
location: https://synbiohub.org/public/igem/BBa_K266011/annotation2262983/range2262983/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K266011/annotation2262983/range2262983/1
type: Range
persistent identity: range2262983
display id: range2262983
version: 1
direction: direction/reverse
top level: BBa_K266011
start: 1
end: 1371
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2262985
display id: annotation2262985
version: 1
top level: BBa_K266011
location: https://synbiohub.org/public/igem/BBa_K266011/annotation2262985/range2262985/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K266011/annotation2262985/range2262985/1
type: Range
persistent identity: range2262985
display id: range2262985
version: 1
direction: direction/reverse
top level: BBa_K266011
start: 1380
end: 1391
orientation: inline

BBa_K091143
type: ComponentDefinition
persistent identity: BBa K091143
display id: BBa_K091143
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas/cI Hybrid Promoter
creator: Xiao Zhu
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: this hybrid promoter is designed to be consecutively off.It combines with plasR(R0079) and lambda cI OR1(see C0051).Adding P-AI-1 sould activate the promoter, while repressed by lambda cI.
mutable notes: "CI binding of the OR region. It binds OR1 with highest affinity, OR2 with next highest, and OR3 with lowest"(http://www.nonanone.com/qw/files/Biology%204450/test2_lecture8,9.txt)
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: plasR OP2
sequence annotation: plasR OP1
sequence annotation: cI OR1
sequence: https://synbiohub.org/public/igem/BBa_K091143_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1967105
display id: annotation1967105
version: 1
top level: BBa_K091143
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967105/range1967105/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967105/range1967105/1
type: Range
persistent identity: range1967105
display id: range1967105
version: 1
direction: direction/forward
top level: BBa_K091143
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967104
display id: annotation1967104
version: 1
top level: BBa_K091143
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967104/range1967104/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967104/range1967104/1
type: Range
persistent identity: range1967104
display id: range1967104
version: 1
direction: direction/forward
top level: BBa_K091143
start: 140
end: 145
orientation: inline

plasR OP2
type: SequenceAnnotation
persistent identity: annotation1967108
display id: annotation1967108
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967108/range1967108/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967108/range1967108/1
type: Range
persistent identity: range1967108
display id: range1967108
version: 1
direction: direction/forward
top level: BBa_K091143
start: 106
end: 126
orientation: inline

plasR OP1
type: SequenceAnnotation
persistent identity: annotation1967107
display id: annotation1967107
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967107/range1967107/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967107/range1967107/1
type: Range
persistent identity: range1967107
display id: range1967107
version: 1
direction: direction/forward
top level: BBa_K091143
start: 46
end: 63
orientation: inline

cI OR1
type: SequenceAnnotation
persistent identity: annotation1967106
display id: annotation1967106
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967106/range1967106/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967106/range1967106/1
type: Range
persistent identity: range1967106
display id: range1967106
version: 1
direction: direction/forward
top level: BBa_K091143
start: 147
end: 164
orientation: inline

BBa_K581000
type: ComponentDefinition
persistent identity: BBa K581000
display id: BBa_K581000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sgrS1+Terminator (small RNA regulator, conjugate part of ptsG1)
creator: Chen Yiwei
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _752_
m user id: 0
owner id: 4411
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part acts as a small regulatory RNA in E.coli.
mutable notes: An RNase E site was placed upstream.
mutable provenance: E.coli K12 strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: G178C mutant of SgrS(wt)
sequence annotation: critical region of SgrS/ptsG interaction
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K581000_sequence/1

G178C mutant of SgrS(wt)
type: SequenceAnnotation
persistent identity: annotation2152330
display id: annotation2152330
version: 1
top level: BBa_K581000
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K581000/annotation2152330/range2152330/1

https://synbiohub.org/public/igem/BBa_K581000/annotation2152330/range2152330/1
type: Range
persistent identity: range2152330
display id: range2152330
version: 1
direction: direction/forward
top level: BBa_K581000
start: 178
end: 178
orientation: inline

critical region of SgrS/ptsG interaction
type: SequenceAnnotation
persistent identity: annotation2130113
display id: annotation2130113
version: 1
top level: BBa_K581000
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K581000/annotation2130113/range2130113/1

https://synbiohub.org/public/igem/BBa_K581000/annotation2130113/range2130113/1
type: Range
persistent identity: range2130113
display id: range2130113
version: 1
direction: direction/forward
top level: BBa_K581000
start: 174
end: 179
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2152356
display id: annotation2152356
version: 1
top level: BBa_K581000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K581000/annotation2152356/range2152356/1

https://synbiohub.org/public/igem/BBa_K581000/annotation2152356/range2152356/1
type: Range
persistent identity: range2152356
display id: range2152356
version: 1
direction: direction/forward
top level: BBa_K581000
start: 236
end: 364
orientation: inline

BBa_K581005
type: ComponentDefinition
persistent identity: BBa K581005
display id: BBa_K581005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pc+sgrS(wt)+Terminator (small RNA regulator, conjugate part of ptsG(wt))
creator: Qin Xiao
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _752_
m user id: 0
owner id: 8589
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the conjugate part of ptsG_wt.SgrS is the wild type small RNA regulator.
mutable notes: wild type.
mutable provenance: Escherichia coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: J23106
sequence annotation: Critical region of SgrS(wt) complementary to ptsG(wt)
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K581005_sequence/1

J23106
type: SequenceAnnotation
persistent identity: annotation2152328
display id: annotation2152328
version: 1
top level: BBa_K581005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K581005/annotation2152328/range2152328/1

https://synbiohub.org/public/igem/BBa_K581005/annotation2152328/range2152328/1
type: Range
persistent identity: range2152328
display id: range2152328
version: 1
direction: direction/forward
top level: BBa_K581005
start: 1
end: 35
orientation: inline

Critical region of SgrS(wt) complementary to ptsG(wt)
type: SequenceAnnotation
persistent identity: annotation2152329
display id: annotation2152329
version: 1
top level: BBa_K581005
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K581005/annotation2152329/range2152329/1

https://synbiohub.org/public/igem/BBa_K581005/annotation2152329/range2152329/1
type: Range
persistent identity: range2152329
display id: range2152329
version: 1
direction: direction/forward
top level: BBa_K581005
start: 216
end: 221
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2152357
display id: annotation2152357
version: 1
top level: BBa_K581005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K581005/annotation2152357/range2152357/1

https://synbiohub.org/public/igem/BBa_K581005/annotation2152357/range2152357/1
type: Range
persistent identity: range2152357
display id: range2152357
version: 1
direction: direction/forward
top level: BBa_K581005
start: 279
end: 407
orientation: inline

BBa_R2108
type: ComponentDefinition
persistent identity: BBa R2108
display id: BBa_R2108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: Placement of the operator sites was optimized for steric interference between the C2003 and RNA polymerase based on structural models.
mutable provenance: This promoter was rationally designed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 site
sequence annotation: -10 site
sequence annotation: transcription start site
sequence annotation: operator site for C2003
sequence: https://synbiohub.org/public/igem/BBa_R2108_sequence/1

-35 site
type: SequenceAnnotation
persistent identity: annotation1903908
display id: annotation1903908
version: 1
top level: BBa_R2108
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903908/range1903908/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903908/range1903908/1
type: Range
persistent identity: range1903908
display id: range1903908
version: 1
direction: direction/forward
top level: BBa_R2108
start: 17
end: 22
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903910
display id: annotation1903910
version: 1
top level: BBa_R2108
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903910/range1903910/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903910/range1903910/1
type: Range
persistent identity: range1903910
display id: range1903910
version: 1
direction: direction/forward
top level: BBa_R2108
start: 40
end: 45
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903911
display id: annotation1903911
version: 1
top level: BBa_R2108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903911/range1903911/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903911/range1903911/1
type: Range
persistent identity: range1903911
display id: range1903911
version: 1
direction: direction/forward
top level: BBa_R2108
start: 53
end: 53
orientation: inline

operator site for C2003
type: SequenceAnnotation
persistent identity: annotation1903909
display id: annotation1903909
version: 1
top level: BBa_R2108
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903909/range1903909/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903909/range1903909/1
type: Range
persistent identity: range1903909
display id: range1903909
version: 1
direction: direction/forward
top level: BBa_R2108
start: 32
end: 43
orientation: inline

BBa_K101000
type: ComponentDefinition
persistent identity: BBa K101000
display id: BBa_K101000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for p22 mnt and TetR
creator: Bennett Swiniarski
created: 2008-07-06T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a promoter that is repressed by both p22 mnt and TetR. It can be used to control the response of any gene that follows this promoter. It will be repressed by the presence of either p22 mnt or TetR.
mutable notes: TetR usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. However, p22 mnt also has two operator sites, one between -35 and -10 and another downstream from -10. Since each had an operator site in between -35 and -10, we had to eliminate the TetR operator site in order to allow the promoter to work. TetR was picked because it has a stronger binding than p22 mnt, and so would be more able to use only one site to bind.
mutable provenance: This part was designed by combining sequences from parts C0040 (TetR promoter) and C0072 (p22 mnt promoter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101000_sequence/1

BBa_K259013
type: ComponentDefinition
persistent identity: BBa K259013
display id: BBa_K259013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC-RBS-GFP-Terminator
creator: Petros Mina
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A part used to quantify the AraC-RBS construct K259007 created by the BCCS-Bristol iGEM'09 team.
mutable notes: RFC10 assembly
mutable provenance: This part was designed with the specified biobricks from the parts registry. AraC occurs naturally in E.coli. GFP occurs in jellyfish species, RBS designed by the Anderson family and Terminator by Resma Shetty.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61100
component: BBa_R0080
component: BBa_E0040
component: BBa_R0081
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_J61100
sequence annotation: BBa_R0081
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_R0080
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K259013_sequence/1

BBa_J61100
type: Component
persistent identity: component2041444
display id: component2041444
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_R0080
type: Component
persistent identity: component2041443
display id: component2041443
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_E0040
type: Component
persistent identity: component2041446
display id: component2041446
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0081
type: Component
persistent identity: component2041436
display id: component2041436
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_B0011
type: Component
persistent identity: component2041451
display id: component2041451
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2041447
display id: component2041447
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2041444
display id: annotation2041444
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
type: Range
persistent identity: range2041444
display id: range2041444
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 349
end: 360
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2041436
display id: annotation2041436
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
type: Range
persistent identity: range2041436
display id: range2041436
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 1
end: 183
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2041447
display id: annotation2041447
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
type: Range
persistent identity: range2041447
display id: range2041447
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1095
end: 1135
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2041451
display id: annotation2041451
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
type: Range
persistent identity: range2041451
display id: range2041451
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1144
end: 1189
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2041443
display id: annotation2041443
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
type: Range
persistent identity: range2041443
display id: range2041443
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 192
end: 340
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2041446
display id: annotation2041446
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
type: Range
persistent identity: range2041446
display id: range2041446
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 367
end: 1086
orientation: inline

BBa_J72005
type: ComponentDefinition
persistent identity: BBa J72005
display id: BBa_J72005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {Ptet} promoter in BBb
creator: John Anderson
created: 2008-07-15T11:00:00Z
modified: 2015-05-08T01:08:26Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 483
owning group id: 171
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Equivalent part to r0040, but in BBb format. In house, part is referred to as pBca9145-Bca1091 {Ptet}. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR ca1091F/R on pSB1A2-r0040 (~100 bp, EcoRI/BamHI) Sub into pBca9145-Bca1089 (EcoRI/BamHI) Product is pBca9145-Bca1091 ---- ca1091F Forward EcoRI/BglII for Ptet part ttctggaattcatgAGATCTtccctatcagtgatagag ca1091R Reverse BamHI for Ptet part GtttGGATCCgtgctcagtatctctatc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J72005_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732400
display id: BBa_I732400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D062NUL)
creator: Zhan Jian
created: 2007-10-03T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: P_template1 serves as the truss for PCR cloning. By respectively applying 7 different primers upstream and downstream, that is, 49 pairs of primers in total, we have got 49 different promoters. Then we made them digsted and loaded into a vector that has already possessed a lacZ-alpha fragment. In this way, we can read from the color of the clones on the plate whether the inserted promoter works or not. By this means, We can try seven different positions for Operator A and seven for Operator B, thus constructing 7*7 kinds of promoters which have actually constituted a specific promoter family. After transformed into the four test plasmids (pCR00, pCR01, pCR10, pCR11) together with a downstream double-reporter system, we will get to know the solo-repression and co-repression effects of the two repressors on the specific promoter.
mutable notes: None.
mutable provenance: None.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732400_sequence/1

BBa_K086021
type: ComponentDefinition
persistent identity: BBa K086021
display id: BBa_K086021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: Lac O and sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_4
sequence: https://synbiohub.org/public/igem/BBa_K086021_sequence/1

P24_4
type: SequenceAnnotation
persistent identity: annotation1995755
display id: annotation1995755
version: 1
top level: BBa_K086021
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086021/annotation1995755/range1995755/1

https://synbiohub.org/public/igem/BBa_K086021/annotation1995755/range1995755/1
type: Range
persistent identity: range1995755
display id: range1995755
version: 1
direction: direction/forward
top level: BBa_K086021
start: 1
end: 55
orientation: inline

BBa_K389050
type: ComponentDefinition
persistent identity: BBa K389050
display id: BBa_K389050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tightly controlled lac operon
creator: Jonas Aretz
created: 2010-09-27T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This BioBrick is a tightly controlled ''lac'' operon. It contains a lacIq and a ''lac'' operator, so it is inducible with IPTG but has a low basal expression. It is also possible to use this BioBrick in strains that do not carry a lacIq like ''E. coli'' TOP10.
mutable notes: we needed a tightly controlled inducible promoter
mutable provenance: partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I14032
component: BBa_R0010
component: BBa_B0034
component: BBa_B0017
component: BBa_C0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I14032
sequence annotation: BBa_C0012
sequence annotation: BBa_R0010
sequence annotation: BBa_B0017
sequence: https://synbiohub.org/public/igem/BBa_K389050_sequence/1

BBa_I14032
type: Component
persistent identity: component2263600
display id: component2263600
version: 1
top level: BBa_K389050
access: public
definition: P(Lac) IQ

BBa_R0010
type: Component
persistent identity: component2263612
display id: component2263612
version: 1
top level: BBa_K389050
access: public
definition: LacI

BBa_B0034
type: Component
persistent identity: component2263602
display id: component2263602
version: 1
top level: BBa_K389050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0017
type: Component
persistent identity: component2263611
display id: component2263611
version: 1
top level: BBa_K389050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_C0012
type: Component
persistent identity: component2263604
display id: component2263604
version: 1
top level: BBa_K389050
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2263602
display id: annotation2263602
version: 1
top level: BBa_K389050
location: https://synbiohub.org/public/igem/BBa_K389050/annotation2263602/range2263602/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389050/annotation2263602/range2263602/1
type: Range
persistent identity: range2263602
display id: range2263602
version: 1
direction: direction/reverse
top level: BBa_K389050
start: 46
end: 57
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2263600
display id: annotation2263600
version: 1
top level: BBa_K389050
location: https://synbiohub.org/public/igem/BBa_K389050/annotation2263600/range2263600/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K389050/annotation2263600/range2263600/1
type: Range
persistent identity: range2263600
display id: range2263600
version: 1
direction: direction/reverse
top level: BBa_K389050
start: 1
end: 37
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2263604
display id: annotation2263604
version: 1
top level: BBa_K389050
location: https://synbiohub.org/public/igem/BBa_K389050/annotation2263604/range2263604/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K389050/annotation2263604/range2263604/1
type: Range
persistent identity: range2263604
display id: range2263604
version: 1
direction: direction/forward
top level: BBa_K389050
start: 64
end: 1191
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2263612
display id: annotation2263612
version: 1
top level: BBa_K389050
location: https://synbiohub.org/public/igem/BBa_K389050/annotation2263612/range2263612/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K389050/annotation2263612/range2263612/1
type: Range
persistent identity: range2263612
display id: range2263612
version: 1
direction: direction/forward
top level: BBa_K389050
start: 1401
end: 1600
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2263611
display id: annotation2263611
version: 1
top level: BBa_K389050
location: https://synbiohub.org/public/igem/BBa_K389050/annotation2263611/range2263611/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389050/annotation2263611/range2263611/1
type: Range
persistent identity: range2263611
display id: range2263611
version: 1
direction: direction/reverse
top level: BBa_K389050
start: 1225
end: 1392
orientation: inline

BBa_K086031
type: ComponentDefinition
persistent identity: BBa K086031
display id: BBa_K086031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: Cleaned
mutable provenance: Lac O and sigma 38 binding sites conserved sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_2
sequence: https://synbiohub.org/public/igem/BBa_K086031_sequence/1

P38_2
type: SequenceAnnotation
persistent identity: annotation1995765
display id: annotation1995765
version: 1
top level: BBa_K086031
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086031/annotation1995765/range1995765/1

https://synbiohub.org/public/igem/BBa_K086031/annotation1995765/range1995765/1
type: Range
persistent identity: range1995765
display id: range1995765
version: 1
direction: direction/forward
top level: BBa_K086031
start: 1
end: 55
orientation: inline

BBa_K165000
type: ComponentDefinition
persistent identity: BBa K165000
display id: BBa_K165000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MET 25 Promoter
creator: John Szymanski
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The is a repressable promoter for use in Saccharomyces cerevisiae. It is repressed by the presence of methionine. When unrepresssed, is has a moderate level of activity.
mutable notes: None.
mutable provenance: From the Saccharomyces cerevisiae genome, by way of Caroline Ajo-Franklin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165000_sequence/1

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732419
display id: BBa_I732419
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732419_sequence/1

BBa_K137049
type: ComponentDefinition
persistent identity: BBa K137049
display id: BBa_K137049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 450 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 450 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promotoer
sequence: https://synbiohub.org/public/igem/BBa_K137049_sequence/1

tetR promotoer
type: SequenceAnnotation
persistent identity: annotation1968022
display id: annotation1968022
version: 1
top level: BBa_K137049
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137049/annotation1968022/range1968022/1

https://synbiohub.org/public/igem/BBa_K137049/annotation1968022/range1968022/1
type: Range
persistent identity: range1968022
display id: range1968022
version: 1
direction: direction/reverse
top level: BBa_K137049
start: 1
end: 54
orientation: inline

Pnot(O11)
type: ComponentDefinition
persistent identity: BBa I732201
display id: BBa_I732201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O11)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732201_sequence/1

BBa_K137048
type: ComponentDefinition
persistent identity: BBa K137048
display id: BBa_K137048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 350 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 350 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137048_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968021
display id: annotation1968021
version: 1
top level: BBa_K137048
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137048/annotation1968021/range1968021/1

https://synbiohub.org/public/igem/BBa_K137048/annotation1968021/range1968021/1
type: Range
persistent identity: range1968021
display id: range1968021
version: 1
direction: direction/reverse
top level: BBa_K137048
start: 1
end: 54
orientation: inline

BBa_J15301
type: ComponentDefinition
persistent identity: BBa J15301
display id: BBa_J15301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pars promoter from Escherichia coli chromosomal ars operon.
creator: Chris French
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the promoter region of the Escherichia coli chromosomal ars promoter. It is repressed by ArsR in the absence of arsenate or arsenite (Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056).
mutable notes: No modifications were made to the sequence. There appears to be an unexpected repeat of ACT immediately before the SpeI site. This does not affect the biobrick suffix but means that the suffix is 5 bases downstream of the annotated transcriptional start site rather than 2 as recommended. The reason for this is unclear.
mutable provenance: Escherichia coli K12. Sequence obtained from X80057 (gi:510824). Regulatory sequence annotation from Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: ArsR binding
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_J15301_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1938076
display id: annotation1938076
version: 1
top level: BBa_J15301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938076/range1938076/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938076/range1938076/1
type: Range
persistent identity: range1938076
display id: range1938076
version: 1
direction: direction/forward
top level: BBa_J15301
start: 88
end: 93
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1938077
display id: annotation1938077
version: 1
top level: BBa_J15301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938077/range1938077/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938077/range1938077/1
type: Range
persistent identity: range1938077
display id: range1938077
version: 1
direction: direction/forward
top level: BBa_J15301
start: 111
end: 116
orientation: inline

ArsR binding
type: SequenceAnnotation
persistent identity: annotation1938074
display id: annotation1938074
version: 1
top level: BBa_J15301
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938074/range1938074/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938074/range1938074/1
type: Range
persistent identity: range1938074
display id: range1938074
version: 1
direction: direction/forward
top level: BBa_J15301
start: 67
end: 71
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1938078
display id: annotation1938078
version: 1
top level: BBa_J15301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938078/range1938078/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938078/range1938078/1
type: Range
persistent identity: range1938078
display id: range1938078
version: 1
direction: direction/forward
top level: BBa_J15301
start: 123
end: 123
orientation: inline

ArsR binding
type: SequenceAnnotation
persistent identity: annotation1938075
display id: annotation1938075
version: 1
top level: BBa_J15301
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938075/range1938075/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938075/range1938075/1
type: Range
persistent identity: range1938075
display id: range1938075
version: 1
direction: direction/forward
top level: BBa_J15301
start: 81
end: 85
orientation: inline

Pnot(O46)
type: ComponentDefinition
persistent identity: BBa I732277
display id: BBa_I732277
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O46)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732277_sequence/1

BBa_K1031744
type: ComponentDefinition
persistent identity: BBa K1031744
display id: BBa_K1031744
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-PaaX
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-PaaX-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_J23114
sequence annotation: BBa_B0034
sequence annotation: PaaX
sequence: https://synbiohub.org/public/igem/BBa_K1031744_sequence/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2357494
display id: annotation2357494
version: 1
top level: BBa_K1031744
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031744/annotation2357494/range2357494/1

https://synbiohub.org/public/igem/BBa_K1031744/annotation2357494/range2357494/1
type: Range
persistent identity: range2357494
display id: range2357494
version: 1
direction: direction/forward
top level: BBa_K1031744
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357495
display id: annotation2357495
version: 1
top level: BBa_K1031744
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031744/annotation2357495/range2357495/1

https://synbiohub.org/public/igem/BBa_K1031744/annotation2357495/range2357495/1
type: Range
persistent identity: range2357495
display id: range2357495
version: 1
direction: direction/forward
top level: BBa_K1031744
start: 44
end: 55
orientation: inline

PaaX
type: SequenceAnnotation
persistent identity: annotation2357496
display id: annotation2357496
version: 1
top level: BBa_K1031744
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031744/annotation2357496/range2357496/1

https://synbiohub.org/public/igem/BBa_K1031744/annotation2357496/range2357496/1
type: Range
persistent identity: range2357496
display id: range2357496
version: 1
direction: direction/forward
top level: BBa_K1031744
start: 62
end: 1012
orientation: inline

BBa_K112708
type: ComponentDefinition
persistent identity: BBa K112708
display id: BBa_K112708
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PfhuA
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The fhuA promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of <PfhuA> basic part K112708 PCR of dv019/dv020 on MG1655 (225bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+225, L) Product is pBca1256-K112708 ----------------------------------------------- dv019 Forward biobricking of <PfhuA> cgataGAATTCatgAGATCTgctaagcgtgaaataccggatgg dv020 Reverse biobricking of <PfhuA> cgttaGGATCCactctgatgtaaagtgaatgataacg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112708_sequence/1

BBa_R2113
type: ComponentDefinition
persistent identity: BBa R2113
display id: BBa_R2113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was designed rationally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: transcription start site
sequence annotation: -10 site
sequence annotation: -35 site
sequence annotation: C2003 operator site
sequence: https://synbiohub.org/public/igem/BBa_R2113_sequence/1

transcription start site
type: SequenceAnnotation
persistent identity: annotation1904657
display id: annotation1904657
version: 1
top level: BBa_R2113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904657/range1904657/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904657/range1904657/1
type: Range
persistent identity: range1904657
display id: range1904657
version: 1
direction: direction/forward
top level: BBa_R2113
start: 53
end: 53
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1904656
display id: annotation1904656
version: 1
top level: BBa_R2113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904656/range1904656/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904656/range1904656/1
type: Range
persistent identity: range1904656
display id: range1904656
version: 1
direction: direction/forward
top level: BBa_R2113
start: 40
end: 45
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1904655
display id: annotation1904655
version: 1
top level: BBa_R2113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904655/range1904655/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904655/range1904655/1
type: Range
persistent identity: range1904655
display id: range1904655
version: 1
direction: direction/forward
top level: BBa_R2113
start: 17
end: 22
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1904658
display id: annotation1904658
version: 1
top level: BBa_R2113
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904658/range1904658/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904658/range1904658/1
type: Range
persistent identity: range1904658
display id: range1904658
version: 1
direction: direction/forward
top level: BBa_R2113
start: 21
end: 32
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732425
display id: BBa_I732425
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: true
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732425_sequence/1

Pnot(O22)
type: ComponentDefinition
persistent identity: BBa I732202
display id: BBa_I732202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O22)
creator: Zhan Jian
created: 2007-08-11T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732202_sequence/1

Pnot(O12)
type: ComponentDefinition
persistent identity: BBa I732270
display id: BBa_I732270
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O12)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732270_sequence/1

BBa_K598020
type: ComponentDefinition
persistent identity: BBa K598020
display id: BBa_K598020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015+pBAD+vioAB+B0015+pBAD+TPP Up-regulated Hammerhead Ribozyme 1.20+vioD+B0015+pBAD+vioE
creator: Zhenrun ZHANG
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8606
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0015+pBAD+vioAB+B0015+pBAD+TPP ribozyme 1.20+vioD+B0015+pBAD+vioE
mutable notes: The Ribosomal Binding Site of vioD is contained in TPP ribozyme 1.20.
mutable provenance: The hammerhead ribozyme comes from E. coli, and the TPP aptamer comes from B. subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: vioB
sequence annotation: pBAD
sequence annotation: rbs
sequence annotation: B0015
sequence annotation: vioE
sequence annotation: vioA
sequence annotation: TPP ribozyme 1.20
sequence annotation: vioD
sequence: https://synbiohub.org/public/igem/BBa_K598020_sequence/1

vioB
type: SequenceAnnotation
persistent identity: annotation2154542
display id: annotation2154542
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154542/range2154542/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154542/range2154542/1
type: Range
persistent identity: range2154542
display id: range2154542
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1559
end: 4556
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154577
display id: annotation2154577
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154577/range2154577/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154577/range2154577/1
type: Range
persistent identity: range2154577
display id: range2154577
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6342
end: 6471
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154516
display id: annotation2154516
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154516/range2154516/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154516/range2154516/1
type: Range
persistent identity: range2154516
display id: range2154516
version: 1
direction: direction/forward
top level: BBa_K598020
start: 273
end: 279
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154514
display id: annotation2154514
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154514/range2154514/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154514/range2154514/1
type: Range
persistent identity: range2154514
display id: range2154514
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1
end: 129
orientation: inline

vioE
type: SequenceAnnotation
persistent identity: annotation2154586
display id: annotation2154586
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154586/range2154586/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154586/range2154586/1
type: Range
persistent identity: range2154586
display id: range2154586
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6489
end: 7064
orientation: inline

vioA
type: SequenceAnnotation
persistent identity: annotation2154517
display id: annotation2154517
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154517/range2154517/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154517/range2154517/1
type: Range
persistent identity: range2154517
display id: range2154517
version: 1
direction: direction/forward
top level: BBa_K598020
start: 285
end: 1542
orientation: inline

TPP ribozyme 1.20
type: SequenceAnnotation
persistent identity: annotation2154556
display id: annotation2154556
version: 1
top level: BBa_K598020
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154556/range2154556/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154556/range2154556/1
type: Range
persistent identity: range2154556
display id: range2154556
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4838
end: 4987
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154548
display id: annotation2154548
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154548/range2154548/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154548/range2154548/1
type: Range
persistent identity: range2154548
display id: range2154548
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4702
end: 4831
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154518
display id: annotation2154518
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154518/range2154518/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154518/range2154518/1
type: Range
persistent identity: range2154518
display id: range2154518
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1545
end: 1551
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154545
display id: annotation2154545
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154545/range2154545/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154545/range2154545/1
type: Range
persistent identity: range2154545
display id: range2154545
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4565
end: 4693
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154583
display id: annotation2154583
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154583/range2154583/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154583/range2154583/1
type: Range
persistent identity: range2154583
display id: range2154583
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6205
end: 6333
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154515
display id: annotation2154515
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154515/range2154515/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154515/range2154515/1
type: Range
persistent identity: range2154515
display id: range2154515
version: 1
direction: direction/forward
top level: BBa_K598020
start: 138
end: 267
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154575
display id: annotation2154575
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154575/range2154575/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154575/range2154575/1
type: Range
persistent identity: range2154575
display id: range2154575
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4976
end: 4982
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154588
display id: annotation2154588
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154588/range2154588/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154588/range2154588/1
type: Range
persistent identity: range2154588
display id: range2154588
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6477
end: 6483
orientation: inline

vioD
type: SequenceAnnotation
persistent identity: annotation2154576
display id: annotation2154576
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154576/range2154576/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154576/range2154576/1
type: Range
persistent identity: range2154576
display id: range2154576
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4988
end: 6112
orientation: inline

BBa_R2109
type: ComponentDefinition
persistent identity: BBa R2109
display id: BBa_R2109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: Placement of the operator site was determined to optimize steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was rationally designed and synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 site
sequence annotation: operator site for C2003
sequence annotation: transcription start site
sequence annotation: -10 site
sequence: https://synbiohub.org/public/igem/BBa_R2109_sequence/1

-35 site
type: SequenceAnnotation
persistent identity: annotation1903912
display id: annotation1903912
version: 1
top level: BBa_R2109
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903912/range1903912/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903912/range1903912/1
type: Range
persistent identity: range1903912
display id: range1903912
version: 1
direction: direction/forward
top level: BBa_R2109
start: 17
end: 22
orientation: inline

operator site for C2003
type: SequenceAnnotation
persistent identity: annotation1903915
display id: annotation1903915
version: 1
top level: BBa_R2109
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903915/range1903915/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903915/range1903915/1
type: Range
persistent identity: range1903915
display id: range1903915
version: 1
direction: direction/forward
top level: BBa_R2109
start: 22
end: 33
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903914
display id: annotation1903914
version: 1
top level: BBa_R2109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903914/range1903914/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903914/range1903914/1
type: Range
persistent identity: range1903914
display id: range1903914
version: 1
direction: direction/forward
top level: BBa_R2109
start: 53
end: 53
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903913
display id: annotation1903913
version: 1
top level: BBa_R2109
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903913/range1903913/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903913/range1903913/1
type: Range
persistent identity: range1903913
display id: range1903913
version: 1
direction: direction/forward
top level: BBa_R2109
start: 40
end: 45
orientation: inline

BBa_J54120
type: ComponentDefinition
persistent identity: BBa J54120
display id: BBa_J54120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EmrR_regulated promoter
creator: Yusaku Nakashima
created: 2006-10-20T11:00:00Z
modified: 2015-08-31T03:48:12Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: EmrR_regulated promoter
mutable notes: AS EmrR regulated
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: BetI_regulated promoter
sequence: https://synbiohub.org/public/igem/BBa_J54120_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1903850
display id: annotation1903850
version: 1
top level: BBa_J54120
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54120/annotation1903850/range1903850/1

https://synbiohub.org/public/igem/BBa_J54120/annotation1903850/range1903850/1
type: Range
persistent identity: range1903850
display id: range1903850
version: 1
direction: direction/forward
top level: BBa_J54120
start: 1
end: 5
orientation: inline

BetI_regulated promoter
type: SequenceAnnotation
persistent identity: annotation1903851
display id: annotation1903851
version: 1
top level: BBa_J54120
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J54120/annotation1903851/range1903851/1

https://synbiohub.org/public/igem/BBa_J54120/annotation1903851/range1903851/1
type: Range
persistent identity: range1903851
display id: range1903851
version: 1
direction: direction/forward
top level: BBa_J54120
start: 6
end: 43
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732438
display id: BBa_I732438
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732438_sequence/1

BBa_I732452
type: ComponentDefinition
persistent identity: BBa I732452
display id: BBa_I732452
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O26+D062O61)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732452_sequence/1

BBa_K190023
type: ComponentDefinition
persistent identity: BBa K190023
display id: BBa_K190023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arsenic Promoter (ArsR regulated) with own RBS
creator: Michael Verhoeven
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _306_
m user id: 0
owner id: 4144
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: gxsga
mutable notes: xshsah
mutable provenance: xhashjxsxhas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 and -10 region
sequence annotation: RBS site
sequence: https://synbiohub.org/public/igem/BBa_K190023_sequence/1

-35 and -10 region
type: SequenceAnnotation
persistent identity: annotation2017705
display id: annotation2017705
version: 1
top level: BBa_K190023
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K190023/annotation2017705/range2017705/1

https://synbiohub.org/public/igem/BBa_K190023/annotation2017705/range2017705/1
type: Range
persistent identity: range2017705
display id: range2017705
version: 1
direction: direction/forward
top level: BBa_K190023
start: 38
end: 72
orientation: inline

RBS site
type: SequenceAnnotation
persistent identity: annotation2017706
display id: annotation2017706
version: 1
top level: BBa_K190023
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K190023/annotation2017706/range2017706/1

https://synbiohub.org/public/igem/BBa_K190023/annotation2017706/range2017706/1
type: Range
persistent identity: range2017706
display id: range2017706
version: 1
direction: direction/forward
top level: BBa_K190023
start: 73
end: 81
orientation: inline

BBa_J22052
type: ComponentDefinition
persistent identity: BBa J22052
display id: BBa_J22052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcya
creator: Sugat Dabholkar
created: 2006-09-03T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: false
group u list: _70_44_
m user id: 0
owner id: 439
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a adenylate cyclase promoter that has been reported to oscillate in cell cycle dependant manner.
mutable notes: No design problems
mutable provenance: Ref: J. Biol. Chem., Vol. 260, Issue 5, 3063-3070, 03, 1985 Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein H Aiba [bases -53 to 12]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J22052_sequence/1

BBa_K112118
type: ComponentDefinition
persistent identity: BBa K112118
display id: BBa_K112118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rrnB P1 promoter
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv028/mv029 on rrnB native sequence (721bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112118 ----------------------------------------------- mv028 Forward Biobricking of rrnB P1 promoter cgataGAATTCatgAGATCTatgcactaaaacgtatcctacgcccg mv029 Reverse Biobricking of rrnB P1 promoter cgttaGGATCCcgccttcccgctacagagtcaagc
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112118_sequence/1

TetR-4C
type: ComponentDefinition
persistent identity: BBa I12212
display id: BBa_I12212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR - TetR-4C heterodimer promoter (negative)
creator: rwald
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is repressed by a heterodimer of wild-type TetR and mutant TetR-4C. It differs from wild-type TetR promoter in that the +4 and -4 regions of its O2 binding site have been changed, and the -35 region of the reverse promoter (pA) has been destroyed.
mutable notes: The O2 and O1 mutations are all single point mutations, occuring at the first base which appears listed here.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: O1
sequence annotation: O1 mutation
sequence annotation: TetR O2
sequence annotation: O2 mutation
sequence: https://synbiohub.org/public/igem/BBa_I12212_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1031340
display id: annotation1031340
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031340/range1031340/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031340/range1031340/1
type: Range
persistent identity: range1031340
display id: range1031340
version: 1
direction: direction/forward
top level: TetR-4C
start: 26
end: 30
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031342
display id: annotation1031342
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031342/range1031342/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031342/range1031342/1
type: Range
persistent identity: range1031342
display id: range1031342
version: 1
direction: direction/forward
top level: TetR-4C
start: 45
end: 50
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1031337
display id: annotation1031337
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031337/range1031337/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031337/range1031337/1
type: Range
persistent identity: range1031337
display id: range1031337
version: 1
direction: direction/forward
top level: TetR-4C
start: 1
end: 6
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1031341
display id: annotation1031341
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031341/range1031341/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031341/range1031341/1
type: Range
persistent identity: range1031341
display id: range1031341
version: 1
direction: direction/forward
top level: TetR-4C
start: 32
end: 50
orientation: inline

O1 mutation
type: SequenceAnnotation
persistent identity: annotation1057574
display id: annotation1057574
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1057574/range1057574/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1057574/range1057574/1
type: Range
persistent identity: range1057574
display id: range1057574
version: 1
direction: direction/forward
top level: TetR-4C
start: 37
end: 37
orientation: inline

TetR O2
type: SequenceAnnotation
persistent identity: annotation1031338
display id: annotation1031338
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031338/range1031338/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031338/range1031338/1
type: Range
persistent identity: range1031338
display id: range1031338
version: 1
direction: direction/forward
top level: TetR-4C
start: 2
end: 20
orientation: inline

O2 mutation
type: SequenceAnnotation
persistent identity: annotation1057573
display id: annotation1057573
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1057573/range1057573/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1057573/range1057573/1
type: Range
persistent identity: range1057573
display id: range1057573
version: 1
direction: direction/forward
top level: TetR-4C
start: 7
end: 7
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1031339
display id: annotation1031339
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031339/range1031339/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031339/range1031339/1
type: Range
persistent identity: range1031339
display id: range1031339
version: 1
direction: direction/forward
top level: TetR-4C
start: 22
end: 27
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732440
display id: BBa_I732440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732440_sequence/1

BBa_I766558
type: ComponentDefinition
persistent identity: BBa I766558
display id: BBa_I766558
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pFig1 (Inducible) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Inducible promoter in yeast
mutable notes: none
mutable provenance: endogenous promoter for Fig1 (Factor Induced Gene 1)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766558_sequence/1

BBa_J22126
type: ComponentDefinition
persistent identity: BBa J22126
display id: BBa_J22126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rec A (SOS) promoter
creator: Krithiga Sankaran
created: 2006-09-05T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: false
group u list: _70_
m user id: 0
owner id: 1038
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RecA (SOS)promoter.
mutable notes: The Rec A(SOS) promoter has been PCR amplified from the chromosomal DNA from MG1655(Ecoli).
mutable provenance: NA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J22126_sequence/1

BBa_I739105
type: ComponentDefinition
persistent identity: BBa I739105
display id: BBa_I739105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (LuxR/HSL, positive / cI, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asd
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: R0062
sequence annotation: Scar
sequence annotation: R0051 (2x OR2)
sequence: https://synbiohub.org/public/igem/BBa_I739105_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation1947916
display id: annotation1947916
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1
type: Range
persistent identity: range1947916
display id: range1947916
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 81
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation1947914
display id: annotation1947914
version: 1
top level: BBa_I739105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1
type: Range
persistent identity: range1947914
display id: range1947914
version: 1
direction: direction/forward
top level: BBa_I739105
start: 1
end: 55
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1947917
display id: annotation1947917
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1
type: Range
persistent identity: range1947917
display id: range1947917
version: 1
direction: direction/forward
top level: BBa_I739105
start: 82
end: 99
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1947915
display id: annotation1947915
version: 1
top level: BBa_I739105
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1
type: Range
persistent identity: range1947915
display id: range1947915
version: 1
direction: direction/forward
top level: BBa_I739105
start: 56
end: 63
orientation: inline

R0051 (2x OR2)
type: SequenceAnnotation
persistent identity: annotation1947918
display id: annotation1947918
version: 1
top level: BBa_I739105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1
type: Range
persistent identity: range1947918
display id: range1947918
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 99
orientation: inline

Mnt
type: ComponentDefinition
persistent identity: BBa R0073
display id: BBa_R0073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (Mnt regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable notes: unsure of where -35 site is
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: mnt
sequence annotation: -10
sequence annotation: dimer right half
sequence annotation: dimer left half
sequence: https://synbiohub.org/public/igem/BBa_R0073_sequence/1

mnt
type: SequenceAnnotation
persistent identity: annotation302669
display id: annotation302669
version: 1
top level: Mnt
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0073/annotation302669/range302669/1

https://synbiohub.org/public/igem/BBa_R0073/annotation302669/range302669/1
type: Range
persistent identity: range302669
display id: range302669
version: 1
direction: direction/forward
top level: Mnt
start: 1
end: 67
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318643
display id: annotation318643
version: 1
top level: Mnt
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318643/range318643/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318643/range318643/1
type: Range
persistent identity: range318643
display id: range318643
version: 1
direction: direction/forward
top level: Mnt
start: 47
end: 52
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation318638
display id: annotation318638
version: 1
top level: Mnt
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318638/range318638/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318638/range318638/1
type: Range
persistent identity: range318638
display id: range318638
version: 1
direction: direction/forward
top level: Mnt
start: 32
end: 38
orientation: inline

dimer left half
type: SequenceAnnotation
persistent identity: annotation318636
display id: annotation318636
version: 1
top level: Mnt
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318636/range318636/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318636/range318636/1
type: Range
persistent identity: range318636
display id: range318636
version: 1
direction: direction/forward
top level: Mnt
start: 23
end: 29
orientation: inline

las TetO
type: ComponentDefinition
persistent identity: BBa I14015
display id: BBa_I14015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Las) TetO
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR and 3OC12HSL regulated promoter with a single Tet Operator (binding site for TetR repressor)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: TetR
sequence annotation: R0079
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14015_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1026120
display id: annotation1026120
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1
type: Range
persistent identity: range1026120
display id: range1026120
version: 1
direction: direction/forward
top level: las TetO
start: 117
end: 122
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation1017910
display id: annotation1017910
version: 1
top level: las TetO
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1
type: Range
persistent identity: range1017910
display id: range1017910
version: 1
direction: direction/forward
top level: las TetO
start: 152
end: 170
orientation: inline

R0079
type: SequenceAnnotation
persistent identity: annotation1017909
display id: annotation1017909
version: 1
top level: las TetO
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1
type: Range
persistent identity: range1017909
display id: range1017909
version: 1
direction: direction/forward
top level: las TetO
start: 1
end: 151
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026116
display id: annotation1026116
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1
type: Range
persistent identity: range1026116
display id: range1026116
version: 1
direction: direction/forward
top level: las TetO
start: 140
end: 145
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732424
display id: BBa_I732424
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732424_sequence/1

BBa_K174001
type: ComponentDefinition
persistent identity: BBa K174001
display id: BBa_K174001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose inducible system
creator: The Newcastle 2009 iGEM team
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:10:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _277_
m user id: 0
owner id: 3942
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Comprised of araR binding sites and araE promoter. Regulates the expression of downstream genes with arabinose.
mutable notes: Forward primer used: GATCTG-GAATTCGCGGCCGCTTCTAGAG-CACAGCGTTCTTTGTAAG (Clamp sequence - Standard Biobrick prefix - first 18 base from the Biobrick) Reverse primer used: TGTGAC-ACTAGTA-GCCCTCCCGAATGTTGAG (Clamp sequence - SpeI site - last 18 base from the Biobrick)
mutable provenance: Wild-type Bacillus subtilis 168 strain. 200 bp long bindingsite_promoter_bindingsite_RBS is amplified by PCR with dangling end primers with EcoRI-NotI-XbaI restriction site at 5??? and SpeI at 3??? and inserted into a Biobrick compatible vector. The sequence is taken from wildtype Bacillus subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: rbs
sequence annotation: sigA promoter
sequence annotation: araR binding site
sequence: https://synbiohub.org/public/igem/BBa_K174001_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2027219
display id: annotation2027219
version: 1
top level: BBa_K174001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K174001/annotation2027219/range2027219/1

https://synbiohub.org/public/igem/BBa_K174001/annotation2027219/range2027219/1
type: Range
persistent identity: range2027219
display id: range2027219
version: 1
direction: direction/forward
top level: BBa_K174001
start: 208
end: 214
orientation: inline

sigA promoter
type: SequenceAnnotation
persistent identity: annotation2027217
display id: annotation2027217
version: 1
top level: BBa_K174001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K174001/annotation2027217/range2027217/1

https://synbiohub.org/public/igem/BBa_K174001/annotation2027217/range2027217/1
type: Range
persistent identity: range2027217
display id: range2027217
version: 1
direction: direction/forward
top level: BBa_K174001
start: 80
end: 123
orientation: inline

araR binding site
type: SequenceAnnotation
persistent identity: annotation2027218
display id: annotation2027218
version: 1
top level: BBa_K174001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K174001/annotation2027218/range2027218/1

https://synbiohub.org/public/igem/BBa_K174001/annotation2027218/range2027218/1
type: Range
persistent identity: range2027218
display id: range2027218
version: 1
direction: direction/forward
top level: BBa_K174001
start: 154
end: 178
orientation: inline

araR binding site
type: SequenceAnnotation
persistent identity: annotation2027216
display id: annotation2027216
version: 1
top level: BBa_K174001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K174001/annotation2027216/range2027216/1

https://synbiohub.org/public/igem/BBa_K174001/annotation2027216/range2027216/1
type: Range
persistent identity: range2027216
display id: range2027216
version: 1
direction: direction/forward
top level: BBa_K174001
start: 32
end: 51
orientation: inline

BBa_K137051
type: ComponentDefinition
persistent identity: BBa K137051
display id: BBa_K137051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 850 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 650 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137051_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968024
display id: annotation1968024
version: 1
top level: BBa_K137051
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137051/annotation1968024/range1968024/1

https://synbiohub.org/public/igem/BBa_K137051/annotation1968024/range1968024/1
type: Range
persistent identity: range1968024
display id: range1968024
version: 1
direction: direction/reverse
top level: BBa_K137051
start: 1
end: 54
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732407
display id: BBa_I732407
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D002O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3C5. For more information please refer to BBa_I732401.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: START
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: BamHI
sequence annotation: BBa_I732007
sequence annotation: BBa_B0010
sequence annotation: STOP
sequence annotation: conserved
sequence annotation: lacZ
sequence annotation: BBa_B0034
sequence annotation: stop
sequence annotation: BglII
sequence annotation: BBa_I732006
sequence annotation: BBa_E0044
sequence annotation: stem_loop
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_I732407_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1944484
display id: annotation1944484
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944484/range1944484/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944484/range1944484/1
type: Range
persistent identity: range1944484
display id: range1944484
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944493
display id: annotation1944493
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944493/range1944493/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944493/range1944493/1
type: Range
persistent identity: range1944493
display id: range1944493
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1161
end: 1201
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944495
display id: annotation1944495
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944495/range1944495/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944495/range1944495/1
type: Range
persistent identity: range1944495
display id: range1944495
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1188
end: 1201
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944480
display id: annotation1944480
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944480/range1944480/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944480/range1944480/1
type: Range
persistent identity: range1944480
display id: range1944480
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944481
display id: annotation1944481
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944481/range1944481/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944481/range1944481/1
type: Range
persistent identity: range1944481
display id: range1944481
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944491
display id: annotation1944491
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944491/range1944491/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944491/range1944491/1
type: Range
persistent identity: range1944491
display id: range1944491
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1073
end: 1152
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944486
display id: annotation1944486
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944486/range1944486/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944486/range1944486/1
type: Range
persistent identity: range1944486
display id: range1944486
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944488
display id: annotation1944488
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944488/range1944488/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944488/range1944488/1
type: Range
persistent identity: range1944488
display id: range1944488
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944485
display id: annotation1944485
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944485/range1944485/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944485/range1944485/1
type: Range
persistent identity: range1944485
display id: range1944485
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944489
display id: annotation1944489
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944489/range1944489/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944489/range1944489/1
type: Range
persistent identity: range1944489
display id: range1944489
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944496
display id: annotation1944496
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944496/range1944496/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944496/range1944496/1
type: Range
persistent identity: range1944496
display id: range1944496
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1194
end: 1194
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944479
display id: annotation1944479
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944479/range1944479/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944479/range1944479/1
type: Range
persistent identity: range1944479
display id: range1944479
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944482
display id: annotation1944482
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944482/range1944482/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944482/range1944482/1
type: Range
persistent identity: range1944482
display id: range1944482
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944487
display id: annotation1944487
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944487/range1944487/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944487/range1944487/1
type: Range
persistent identity: range1944487
display id: range1944487
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1944490
display id: annotation1944490
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944490/range1944490/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944490/range1944490/1
type: Range
persistent identity: range1944490
display id: range1944490
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 1064
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944492
display id: annotation1944492
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944492/range1944492/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944492/range1944492/1
type: Range
persistent identity: range1944492
display id: range1944492
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1084
end: 1127
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944483
display id: annotation1944483
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944483/range1944483/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944483/range1944483/1
type: Range
persistent identity: range1944483
display id: range1944483
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944494
display id: annotation1944494
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944494/range1944494/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944494/range1944494/1
type: Range
persistent identity: range1944494
display id: range1944494
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1168
end: 1187
orientation: inline

BBa_K266003
type: ComponentDefinition
persistent identity: BBa K266003
display id: BBa_K266003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: POPS -> Lac Inverter -> LasR
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lac system inverter controlling the expression of LasR
mutable notes: None
mutable provenance: The biobricks BBa_Q04121, BBa_B0034 and BBa_K266002 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0034
component: BBa_Q04121
component: BBa_K266002
sequence annotation: BBa_B0034
sequence annotation: BBa_K266002
sequence annotation: BBa_Q04121
sequence: https://synbiohub.org/public/igem/BBa_K266003_sequence/1

BBa_B0034
type: Component
persistent identity: component2276857
display id: component2276857
version: 1
top level: BBa_K266003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_Q04121
type: Component
persistent identity: component2276855
display id: component2276855
version: 1
top level: BBa_K266003
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_K266002
type: Component
persistent identity: component2276870
display id: component2276870
version: 1
top level: BBa_K266003
access: public
definition: https://synbiohub.org/public/igem/BBa_K266002/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2276857
display id: annotation2276857
version: 1
top level: BBa_K266003
location: https://synbiohub.org/public/igem/BBa_K266003/annotation2276857/range2276857/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K266003/annotation2276857/range2276857/1
type: Range
persistent identity: range2276857
display id: range2276857
version: 1
direction: direction/reverse
top level: BBa_K266003
start: 1380
end: 1391
orientation: inline

BBa_K266002
type: SequenceAnnotation
persistent identity: annotation2276870
display id: annotation2276870
version: 1
top level: BBa_K266003
location: https://synbiohub.org/public/igem/BBa_K266003/annotation2276870/range2276870/1
component: BBa_K266002

https://synbiohub.org/public/igem/BBa_K266003/annotation2276870/range2276870/1
type: Range
persistent identity: range2276870
display id: range2276870
version: 1
direction: direction/reverse
top level: BBa_K266003
start: 1398
end: 2315
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2276855
display id: annotation2276855
version: 1
top level: BBa_K266003
location: https://synbiohub.org/public/igem/BBa_K266003/annotation2276855/range2276855/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K266003/annotation2276855/range2276855/1
type: Range
persistent identity: range2276855
display id: range2276855
version: 1
direction: direction/reverse
top level: BBa_K266003
start: 1
end: 1371
orientation: inline

BBa_K518008
type: ComponentDefinition
persistent identity: BBa K518008
display id: BBa_K518008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG-inducible CheZ repression device
creator: Masato Ohgishi
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility. This device usually allows strong cheZ expression, while in the presense of IPTG, the transcription of cheZ is repressed by the overexpression of lambda cI repressor BBa_C0051.
mutable notes: None.
mutable provenance: cheZ CDS is cloned from Escherichia coli JM109 strain. Other sequences are from BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_C0051
component: BBa_B0032
component: BBa_K518005
component: BBa_R0011
component: BBa_B0014
component: BBa_R0051
sequence annotation: BBa_B0032
sequence annotation: BBa_R0011
sequence annotation: BBa_C0051
sequence annotation: BBa_B0014
sequence annotation: BBa_R0051
sequence annotation: BBa_K518005
sequence: https://synbiohub.org/public/igem/BBa_K518008_sequence/1

BBa_C0051
type: Component
persistent identity: component2289757
display id: component2289757
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0032
type: Component
persistent identity: component2289752
display id: component2289752
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K518005
type: Component
persistent identity: component2289738
display id: component2289738
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_K518005/1

BBa_R0011
type: Component
persistent identity: component2289746
display id: component2289746
version: 1
top level: BBa_K518008
access: public
definition: lacI+pL

BBa_B0014
type: Component
persistent identity: component2289745
display id: component2289745
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: Component
persistent identity: component2289764
display id: component2289764
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0032
type: Component
persistent identity: component2289736
display id: component2289736
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0051
type: Component
persistent identity: component2289731
display id: component2289731
version: 1
top level: BBa_K518008
access: public
definition: cI lam

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2289736
display id: annotation2289736
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289736/range2289736/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518008/annotation2289736/range2289736/1
type: Range
persistent identity: range2289736
display id: range2289736
version: 1
direction: direction/forward
top level: BBa_K518008
start: 58
end: 70
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2289746
display id: annotation2289746
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289746/range2289746/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K518008/annotation2289746/range2289746/1
type: Range
persistent identity: range2289746
display id: range2289746
version: 1
direction: direction/forward
top level: BBa_K518008
start: 995
end: 1048
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2289757
display id: annotation2289757
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289757/range2289757/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K518008/annotation2289757/range2289757/1
type: Range
persistent identity: range2289757
display id: range2289757
version: 1
direction: direction/reverse
top level: BBa_K518008
start: 1077
end: 1851
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2289745
display id: annotation2289745
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289745/range2289745/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518008/annotation2289745/range2289745/1
type: Range
persistent identity: range2289745
display id: range2289745
version: 1
direction: direction/reverse
top level: BBa_K518008
start: 892
end: 986
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2289752
display id: annotation2289752
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289752/range2289752/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518008/annotation2289752/range2289752/1
type: Range
persistent identity: range2289752
display id: range2289752
version: 1
direction: direction/forward
top level: BBa_K518008
start: 1058
end: 1070
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2289764
display id: annotation2289764
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289764/range2289764/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518008/annotation2289764/range2289764/1
type: Range
persistent identity: range2289764
display id: range2289764
version: 1
direction: direction/reverse
top level: BBa_K518008
start: 1860
end: 1954
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2289731
display id: annotation2289731
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289731/range2289731/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K518008/annotation2289731/range2289731/1
type: Range
persistent identity: range2289731
display id: range2289731
version: 1
direction: direction/forward
top level: BBa_K518008
start: 1
end: 49
orientation: inline

BBa_K518005
type: SequenceAnnotation
persistent identity: annotation2289738
display id: annotation2289738
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289738/range2289738/1
component: BBa_K518005

https://synbiohub.org/public/igem/BBa_K518008/annotation2289738/range2289738/1
type: Range
persistent identity: range2289738
display id: range2289738
version: 1
direction: direction/reverse
top level: BBa_K518008
start: 79
end: 883
orientation: inline

BBa_K283011
type: ComponentDefinition
persistent identity: BBa K283011
display id: BBa_K283011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-lpp_omp(46-159)_streptavidin_Histag
creator: Shi Lei, W Ling , Nelson Chu
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The fusion protein omp(46-159)streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The omp domain directs the expression of the fusion protein on the surface membrane
mutable notes: BBa_K283011
mutable provenance: The pTet promoter, rbs are from the registry The fusion protein is self-designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J36837
component: BBa_J36841
component: BBa_J36835
component: BBa_R0040
component: BBa_B0034
sequence annotation: BBa_J36835
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_J36841
sequence annotation: BBa_J36837
sequence: https://synbiohub.org/public/igem/BBa_K283011_sequence/1

BBa_J36837
type: Component
persistent identity: component2055666
display id: component2055666
version: 1
top level: BBa_K283011
access: public
definition: https://synbiohub.org/public/igem/BBa_J36837/1

BBa_J36841
type: Component
persistent identity: component2055667
display id: component2055667
version: 1
top level: BBa_K283011
access: public
definition: https://synbiohub.org/public/igem/BBa_J36841/1

BBa_J36835
type: Component
persistent identity: component2055665
display id: component2055665
version: 1
top level: BBa_K283011
access: public
definition: https://synbiohub.org/public/igem/BBa_J36835/1

BBa_R0040
type: Component
persistent identity: component2055658
display id: component2055658
version: 1
top level: BBa_K283011
access: public
definition: p(tetR)

BBa_B0034
type: Component
persistent identity: component2055664
display id: component2055664
version: 1
top level: BBa_K283011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J36835
type: SequenceAnnotation
persistent identity: annotation2055665
display id: annotation2055665
version: 1
top level: BBa_K283011
location: https://synbiohub.org/public/igem/BBa_K283011/annotation2055665/range2055665/1
component: BBa_J36835

https://synbiohub.org/public/igem/BBa_K283011/annotation2055665/range2055665/1
type: Range
persistent identity: range2055665
display id: range2055665
version: 1
direction: direction/reverse
top level: BBa_K283011
start: 81
end: 167
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2055658
display id: annotation2055658
version: 1
top level: BBa_K283011
location: https://synbiohub.org/public/igem/BBa_K283011/annotation2055658/range2055658/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K283011/annotation2055658/range2055658/1
type: Range
persistent identity: range2055658
display id: range2055658
version: 1
direction: direction/forward
top level: BBa_K283011
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2055664
display id: annotation2055664
version: 1
top level: BBa_K283011
location: https://synbiohub.org/public/igem/BBa_K283011/annotation2055664/range2055664/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283011/annotation2055664/range2055664/1
type: Range
persistent identity: range2055664
display id: range2055664
version: 1
direction: direction/reverse
top level: BBa_K283011
start: 63
end: 74
orientation: inline

BBa_J36841
type: SequenceAnnotation
persistent identity: annotation2055667
display id: annotation2055667
version: 1
top level: BBa_K283011
location: https://synbiohub.org/public/igem/BBa_K283011/annotation2055667/range2055667/1
component: BBa_J36841

https://synbiohub.org/public/igem/BBa_K283011/annotation2055667/range2055667/1
type: Range
persistent identity: range2055667
display id: range2055667
version: 1
direction: direction/reverse
top level: BBa_K283011
start: 526
end: 930
orientation: inline

BBa_J36837
type: SequenceAnnotation
persistent identity: annotation2055666
display id: annotation2055666
version: 1
top level: BBa_K283011
location: https://synbiohub.org/public/igem/BBa_K283011/annotation2055666/range2055666/1
component: BBa_J36837

https://synbiohub.org/public/igem/BBa_K283011/annotation2055666/range2055666/1
type: Range
persistent identity: range2055666
display id: range2055666
version: 1
direction: direction/reverse
top level: BBa_K283011
start: 176
end: 517
orientation: inline

BBa_K113009
type: ComponentDefinition
persistent identity: BBa K113009
display id: BBa_K113009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad/araC
creator: Zheng Wei, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is the same as BBa_I0050. But the part seems not working so we make a new one, which the template is from iHKU team.
mutable notes: later
mutable provenance: iHKU team
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K113009_sequence/1

Las CIO
type: ComponentDefinition
persistent identity: BBa I14016
display id: BBa_I14016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Las) CIO
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR and 3OC12HSL regulated promoter with CI Operator 1 (binding site for lambda cI repressor)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R0079
sequence annotation: -35
sequence annotation: -10
sequence annotation: CI lambda O1
sequence: https://synbiohub.org/public/igem/BBa_I14016_sequence/1

R0079
type: SequenceAnnotation
persistent identity: annotation1017859
display id: annotation1017859
version: 1
top level: Las CIO
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1017859/range1017859/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1017859/range1017859/1
type: Range
persistent identity: range1017859
display id: range1017859
version: 1
direction: direction/forward
top level: Las CIO
start: 1
end: 151
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026124
display id: annotation1026124
version: 1
top level: Las CIO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1026124/range1026124/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1026124/range1026124/1
type: Range
persistent identity: range1026124
display id: range1026124
version: 1
direction: direction/forward
top level: Las CIO
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026125
display id: annotation1026125
version: 1
top level: Las CIO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1026125/range1026125/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1026125/range1026125/1
type: Range
persistent identity: range1026125
display id: range1026125
version: 1
direction: direction/forward
top level: Las CIO
start: 140
end: 145
orientation: inline

CI lambda O1
type: SequenceAnnotation
persistent identity: annotation1017878
display id: annotation1017878
version: 1
top level: Las CIO
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1017878/range1017878/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1017878/range1017878/1
type: Range
persistent identity: range1017878
display id: range1017878
version: 1
direction: direction/forward
top level: Las CIO
start: 152
end: 168
orientation: inline

BBa_I756018
type: ComponentDefinition
persistent identity: BBa I756018
display id: BBa_I756018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda Operator in SV-40 intron
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Lambda Operator in SV-40 intron
mutable notes: Lambda Operator in SV-40 intron
mutable provenance: Lambda Operator in SV-40 intron
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756018_sequence/1

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732429
display id: BBa_I732429
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732429_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732409
display id: BBa_I732409
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D026O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP) loaded on pSB3C5. For more information please refer to BBa_I732403.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: stop
sequence annotation: polya
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: BglII
sequence annotation: BBa_I732007
sequence annotation: lacZ
sequence annotation: BBa_B0010
sequence annotation: START
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BamHI
sequence annotation: STOP
sequence annotation: GFP protein
sequence annotation: T7 TE
sequence annotation: stem_loop
sequence annotation: BBa_I732006
sequence: https://synbiohub.org/public/igem/BBa_I732409_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1944535
display id: annotation1944535
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944535/range1944535/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944535/range1944535/1
type: Range
persistent identity: range1944535
display id: range1944535
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1155
end: 1155
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944534
display id: annotation1944534
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944534/range1944534/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944534/range1944534/1
type: Range
persistent identity: range1944534
display id: range1944534
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1149
end: 1162
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944521
display id: annotation1944521
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944521/range1944521/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944521/range1944521/1
type: Range
persistent identity: range1944521
display id: range1944521
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944520
display id: annotation1944520
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944520/range1944520/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944520/range1944520/1
type: Range
persistent identity: range1944520
display id: range1944520
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944517
display id: annotation1944517
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944517/range1944517/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944517/range1944517/1
type: Range
persistent identity: range1944517
display id: range1944517
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944519
display id: annotation1944519
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944519/range1944519/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944519/range1944519/1
type: Range
persistent identity: range1944519
display id: range1944519
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944527
display id: annotation1944527
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944527/range1944527/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944527/range1944527/1
type: Range
persistent identity: range1944527
display id: range1944527
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944523
display id: annotation1944523
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944523/range1944523/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944523/range1944523/1
type: Range
persistent identity: range1944523
display id: range1944523
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944530
display id: annotation1944530
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944530/range1944530/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944530/range1944530/1
type: Range
persistent identity: range1944530
display id: range1944530
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1034
end: 1113
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944522
display id: annotation1944522
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944522/range1944522/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944522/range1944522/1
type: Range
persistent identity: range1944522
display id: range1944522
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944526
display id: annotation1944526
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944526/range1944526/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944526/range1944526/1
type: Range
persistent identity: range1944526
display id: range1944526
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1944529
display id: annotation1944529
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944529/range1944529/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944529/range1944529/1
type: Range
persistent identity: range1944529
display id: range1944529
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 1025
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944532
display id: annotation1944532
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944532/range1944532/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944532/range1944532/1
type: Range
persistent identity: range1944532
display id: range1944532
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1122
end: 1162
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944518
display id: annotation1944518
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944518/range1944518/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944518/range1944518/1
type: Range
persistent identity: range1944518
display id: range1944518
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944524
display id: annotation1944524
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944524/range1944524/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944524/range1944524/1
type: Range
persistent identity: range1944524
display id: range1944524
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation1944528
display id: annotation1944528
version: 1
top level: U097NUL+D0
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944528/range1944528/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944528/range1944528/1
type: Range
persistent identity: range1944528
display id: range1944528
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 306
end: 1025
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944533
display id: annotation1944533
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944533/range1944533/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944533/range1944533/1
type: Range
persistent identity: range1944533
display id: range1944533
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1129
end: 1148
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944531
display id: annotation1944531
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944531/range1944531/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944531/range1944531/1
type: Range
persistent identity: range1944531
display id: range1944531
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1045
end: 1088
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944525
display id: annotation1944525
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944525/range1944525/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944525/range1944525/1
type: Range
persistent identity: range1944525
display id: range1944525
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pL)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
mutable provenance: V. fischeri.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0063
sequence annotation: Putative LuxR/HSL
sequence annotation: -35
sequence annotation: start
sequence annotation: -10
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 151
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
direction: direction/forward
top level: lux pL
start: 130
end: 149
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
direction: direction/forward
top level: lux pL
start: 89
end: 94
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
direction: direction/forward
top level: lux pL
start: 128
end: 128
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
direction: direction/forward
top level: lux pL
start: 115
end: 122
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 20
orientation: inline

Pnot(O47)
type: ComponentDefinition
persistent identity: BBa I732278
display id: BBa_I732278
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O47)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732278_sequence/1

BBa_I739107
type: ComponentDefinition
persistent identity: BBa I739107
display id: BBa_I739107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (cI, negative / LacI, negative)
creator: Stefan Luzi
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: sadf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R0051
sequence annotation: R0010
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_I739107_sequence/1

R0051
type: SequenceAnnotation
persistent identity: annotation1948510
display id: annotation1948510
version: 1
top level: BBa_I739107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948510/range1948510/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948510/range1948510/1
type: Range
persistent identity: range1948510
display id: range1948510
version: 1
direction: direction/forward
top level: BBa_I739107
start: 1
end: 43
orientation: inline

R0010
type: SequenceAnnotation
persistent identity: annotation1948512
display id: annotation1948512
version: 1
top level: BBa_I739107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948512/range1948512/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948512/range1948512/1
type: Range
persistent identity: range1948512
display id: range1948512
version: 1
direction: direction/forward
top level: BBa_I739107
start: 44
end: 78
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1948511
display id: annotation1948511
version: 1
top level: BBa_I739107
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948511/range1948511/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948511/range1948511/1
type: Range
persistent identity: range1948511
display id: range1948511
version: 1
direction: direction/forward
top level: BBa_I739107
start: 44
end: 78
orientation: inline

BBa_J34806
type: ComponentDefinition
persistent identity: BBa J34806
display id: BBa_J34806
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter lac induced
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be used with transcription factor BBa_J34802
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34806_sequence/1

BBa_I751500
type: ComponentDefinition
persistent identity: BBa I751500
display id: BBa_I751500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcI (for positive control of pcI-lux hybrid promoter)
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pcI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pcI
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence annotation: OR2
sequence annotation: OR3
sequence: https://synbiohub.org/public/igem/BBa_I751500_sequence/1

pcI
type: SequenceAnnotation
persistent identity: annotation1955476
display id: annotation1955476
version: 1
top level: BBa_I751500
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1955476/range1955476/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1955476/range1955476/1
type: Range
persistent identity: range1955476
display id: range1955476
version: 1
direction: direction/forward
top level: BBa_I751500
start: 1
end: 77
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1958479
display id: annotation1958479
version: 1
top level: BBa_I751500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958479/range1958479/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958479/range1958479/1
type: Range
persistent identity: range1958479
display id: range1958479
version: 1
direction: direction/forward
top level: BBa_I751500
start: 21
end: 26
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1958484
display id: annotation1958484
version: 1
top level: BBa_I751500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958484/range1958484/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958484/range1958484/1
type: Range
persistent identity: range1958484
display id: range1958484
version: 1
direction: direction/forward
top level: BBa_I751500
start: 51
end: 77
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1958480
display id: annotation1958480
version: 1
top level: BBa_I751500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958480/range1958480/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958480/range1958480/1
type: Range
persistent identity: range1958480
display id: range1958480
version: 1
direction: direction/forward
top level: BBa_I751500
start: 47
end: 50
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1958483
display id: annotation1958483
version: 1
top level: BBa_I751500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958483/range1958483/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958483/range1958483/1
type: Range
persistent identity: range1958483
display id: range1958483
version: 1
direction: direction/forward
top level: BBa_I751500
start: 27
end: 46
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation1958482
display id: annotation1958482
version: 1
top level: BBa_I751500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958482/range1958482/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958482/range1958482/1
type: Range
persistent identity: range1958482
display id: range1958482
version: 1
direction: direction/forward
top level: BBa_I751500
start: 1
end: 20
orientation: inline

BBa_I766214
type: ComponentDefinition
persistent identity: BBa I766214
display id: BBa_I766214
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGal1
creator: Nili Sommovilla
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pGAl1 promoter region
mutable notes: none
mutable provenance: yeast
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766214_sequence/1

BBa_K266004
type: ComponentDefinition
persistent identity: BBa K266004
display id: BBa_K266004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Const Lac Inverter -> LasR
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Constitutive promoter J23100 with Lac system inverter controlling the expression of LasR
mutable notes: None
mutable provenance: The biobricks BBa_J23100 and BBa_K266003 where used for build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_J23100
component: BBa_K266003
sequence annotation: BBa_J23100
sequence annotation: BBa_K266003
sequence: https://synbiohub.org/public/igem/BBa_K266004_sequence/1

BBa_J23100
type: Component
persistent identity: component2287678
display id: component2287678
version: 1
top level: BBa_K266004
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K266003
type: Component
persistent identity: component2287713
display id: component2287713
version: 1
top level: BBa_K266004
access: public
definition: BBa_K266003

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2287678
display id: annotation2287678
version: 1
top level: BBa_K266004
location: https://synbiohub.org/public/igem/BBa_K266004/annotation2287678/range2287678/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K266004/annotation2287678/range2287678/1
type: Range
persistent identity: range2287678
display id: range2287678
version: 1
direction: direction/reverse
top level: BBa_K266004
start: 1
end: 35
orientation: inline

BBa_K266003
type: SequenceAnnotation
persistent identity: annotation2287713
display id: annotation2287713
version: 1
top level: BBa_K266004
location: https://synbiohub.org/public/igem/BBa_K266004/annotation2287713/range2287713/1
component: BBa_K266003

https://synbiohub.org/public/igem/BBa_K266004/annotation2287713/range2287713/1
type: Range
persistent identity: range2287713
display id: range2287713
version: 1
direction: direction/reverse
top level: BBa_K266004
start: 44
end: 2358
orientation: inline

BBa_K1682002
type: ComponentDefinition
persistent identity: BBa K1682002
display id: BBa_K1682002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>A]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:15:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>A]
mutable notes: kdpFp[-15, T>A]
mutable provenance: kdpFp[-15, T>A]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: EcoRI Illegal site removed T->A
sequence annotation: phospho-KdpE binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K1682002_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2473586
display id: annotation2473586
version: 1
top level: BBa_K1682002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473586/range2473586/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473586/range2473586/1
type: Range
persistent identity: range2473586
display id: range2473586
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 69
end: 74
orientation: inline

EcoRI Illegal site removed T->A
type: SequenceAnnotation
persistent identity: annotation2475205
display id: annotation2475205
version: 1
top level: BBa_K1682002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2475205/range2475205/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2475205/range2475205/1
type: Range
persistent identity: range2475205
display id: range2475205
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 65
end: 66
orientation: inline

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473585
display id: annotation2473585
version: 1
top level: BBa_K1682002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473585/range2473585/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473585/range2473585/1
type: Range
persistent identity: range2473585
display id: range2473585
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 8
end: 24
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473587
display id: annotation2473587
version: 1
top level: BBa_K1682002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473587/range2473587/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473587/range2473587/1
type: Range
persistent identity: range2473587
display id: range2473587
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 42
end: 47
orientation: inline

BBa_K190016
type: ComponentDefinition
persistent identity: BBa K190016
display id: BBa_K190016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zinc Promoter (ZntR regulated)
creator: Michael Verhoeven
created: 2009-07-14T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _306_
m user id: 0
owner id: 4144
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter sequence with recognition site for ZntR transcriptional regulator protein.
mutable notes: None
mutable provenance: E.coli TOP10
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Palindromic binding site for ZntR
sequence annotation: -35 and -10 region
sequence: https://synbiohub.org/public/igem/BBa_K190016_sequence/1

Palindromic binding site for ZntR
type: SequenceAnnotation
persistent identity: annotation2011504
display id: annotation2011504
version: 1
top level: BBa_K190016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K190016/annotation2011504/range2011504/1

https://synbiohub.org/public/igem/BBa_K190016/annotation2011504/range2011504/1
type: Range
persistent identity: range2011504
display id: range2011504
version: 1
direction: direction/forward
top level: BBa_K190016
start: 32
end: 53
orientation: inline

-35 and -10 region
type: SequenceAnnotation
persistent identity: annotation2011503
display id: annotation2011503
version: 1
top level: BBa_K190016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K190016/annotation2011503/range2011503/1

https://synbiohub.org/public/igem/BBa_K190016/annotation2011503/range2011503/1
type: Range
persistent identity: range2011503
display id: range2011503
version: 1
direction: direction/forward
top level: BBa_K190016
start: 35
end: 69
orientation: inline

Pnot(O33)
type: ComponentDefinition
persistent identity: BBa I732203
display id: BBa_I732203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O33)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732203_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732417
display id: BBa_I732417
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732417_sequence/1

BBa_K1031710
type: ComponentDefinition
persistent identity: BBa K1031710
display id: BBa_K1031710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PaaX-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PaaX-TT
mutable notes: Molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0015
sequence annotation: PaaX
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031710_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357487
display id: annotation2357487
version: 1
top level: BBa_K1031710
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357487/range2357487/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357487/range2357487/1
type: Range
persistent identity: range2357487
display id: range2357487
version: 1
direction: direction/forward
top level: BBa_K1031710
start: 978
end: 1106
orientation: inline

PaaX
type: SequenceAnnotation
persistent identity: annotation2357486
display id: annotation2357486
version: 1
top level: BBa_K1031710
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357486/range2357486/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357486/range2357486/1
type: Range
persistent identity: range2357486
display id: range2357486
version: 1
direction: direction/forward
top level: BBa_K1031710
start: 19
end: 969
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357485
display id: annotation2357485
version: 1
top level: BBa_K1031710
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357485/range2357485/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357485/range2357485/1
type: Range
persistent identity: range2357485
display id: range2357485
version: 1
direction: direction/forward
top level: BBa_K1031710
start: 1
end: 12
orientation: inline

BBa_I12036
type: ComponentDefinition
persistent identity: BBa I12036
display id: BBa_I12036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (cooperative repression by 434 cI)
creator: Hans
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operators are used for both activation and repression to enhance cooperativity.
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage.
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR1 lambda
sequence annotation: -10
sequence annotation: OR2 434
sequence annotation: OR2 lambda
sequence annotation: OR1 434
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I12036_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1056739
display id: annotation1056739
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1
type: Range
persistent identity: range1056739
display id: range1056739
version: 1
direction: direction/forward
top level: BBa_I12036
start: 48
end: 53
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1056735
display id: annotation1056735
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1
type: Range
persistent identity: range1056735
display id: range1056735
version: 1
direction: direction/forward
top level: BBa_I12036
start: 9
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1056740
display id: annotation1056740
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1
type: Range
persistent identity: range1056740
display id: range1056740
version: 1
direction: direction/forward
top level: BBa_I12036
start: 71
end: 76
orientation: inline

OR2 434
type: SequenceAnnotation
persistent identity: annotation1056738
display id: annotation1056738
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1
type: Range
persistent identity: range1056738
display id: range1056738
version: 1
direction: direction/forward
top level: BBa_I12036
start: 78
end: 91
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1056736
display id: annotation1056736
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1
type: Range
persistent identity: range1056736
display id: range1056736
version: 1
direction: direction/forward
top level: BBa_I12036
start: 33
end: 49
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation1056737
display id: annotation1056737
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1
type: Range
persistent identity: range1056737
display id: range1056737
version: 1
direction: direction/forward
top level: BBa_I12036
start: 56
end: 69
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1062203
display id: annotation1062203
version: 1
top level: BBa_I12036
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1
type: Range
persistent identity: range1062203
display id: range1062203
version: 1
direction: direction/forward
top level: BBa_I12036
start: 83
end: 83
orientation: inline

Pnot(O16)
type: ComponentDefinition
persistent identity: BBa I732271
display id: BBa_I732271
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O16)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732271_sequence/1

BBa_K581003
type: ComponentDefinition
persistent identity: BBa K581003
display id: BBa_K581003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)
creator: Chen Yiwei, Qin Xiao
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _752_
m user id: 0
owner id: 8589
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the conjugate part of ptsG2-gfp.
mutable notes: Site specifically mutated for biological orthogonality.
mutable provenance: Escherichia coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: G176C mutant of SgrS(wt)
sequence annotation: critical region for SgrS/ptsG interaction
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K581003_sequence/1

G176C mutant of SgrS(wt)
type: SequenceAnnotation
persistent identity: annotation2152333
display id: annotation2152333
version: 1
top level: BBa_K581003
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K581003/annotation2152333/range2152333/1

https://synbiohub.org/public/igem/BBa_K581003/annotation2152333/range2152333/1
type: Range
persistent identity: range2152333
display id: range2152333
version: 1
direction: direction/forward
top level: BBa_K581003
start: 176
end: 176
orientation: inline

critical region for SgrS/ptsG interaction
type: SequenceAnnotation
persistent identity: annotation2152332
display id: annotation2152332
version: 1
top level: BBa_K581003
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K581003/annotation2152332/range2152332/1

https://synbiohub.org/public/igem/BBa_K581003/annotation2152332/range2152332/1
type: Range
persistent identity: range2152332
display id: range2152332
version: 1
direction: direction/forward
top level: BBa_K581003
start: 174
end: 179
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2152345
display id: annotation2152345
version: 1
top level: BBa_K581003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K581003/annotation2152345/range2152345/1

https://synbiohub.org/public/igem/BBa_K581003/annotation2152345/range2152345/1
type: Range
persistent identity: range2152345
display id: range2152345
version: 1
direction: direction/forward
top level: BBa_K581003
start: 236
end: 364
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732422
display id: BBa_I732422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732422_sequence/1

BBa_R0185
type: ComponentDefinition
persistent identity: BBa R0185
display id: BBa_R0185
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -10 base to a G, which should make it about 4% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -10 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -10.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: T7 promoter sequence
sequence: https://synbiohub.org/public/igem/BBa_R0185_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962093
display id: annotation1962093
version: 1
top level: BBa_R0185
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0185/annotation1962093/range1962093/1

https://synbiohub.org/public/igem/BBa_R0185/annotation1962093/range1962093/1
type: Range
persistent identity: range1962093
display id: range1962093
version: 1
direction: direction/forward
top level: BBa_R0185
start: 20
end: 44
orientation: inline

T7 promoter sequence
type: SequenceAnnotation
persistent identity: annotation1962092
display id: annotation1962092
version: 1
top level: BBa_R0185
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0185/annotation1962092/range1962092/1

https://synbiohub.org/public/igem/BBa_R0185/annotation1962092/range1962092/1
type: Range
persistent identity: range1962092
display id: range1962092
version: 1
direction: direction/forward
top level: BBa_R0185
start: 1
end: 20
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12006
display id: BBa_I12006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (repressed by 434 cI)
creator: mcnamara
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
mutable notes: The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
mutable provenance: Bushman(1993), Shih & Gussin (1983)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1 434
sequence annotation: OR2 lambda
sequence annotation: -10
sequence annotation: OR1 lambda
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I12006_sequence/1

OR1 434
type: SequenceAnnotation
persistent identity: annotation786365
display id: annotation786365
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
type: Range
persistent identity: range786365
display id: range786365
version: 1
direction: direction/forward
top level: Prm +
start: 56
end: 69
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation786518
display id: annotation786518
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
type: Range
persistent identity: range786518
display id: range786518
version: 1
direction: direction/forward
top level: Prm +
start: 33
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation837228
display id: annotation837228
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
type: Range
persistent identity: range837228
display id: range837228
version: 1
direction: direction/forward
top level: Prm +
start: 71
end: 76
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation786500
display id: annotation786500
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
type: Range
persistent identity: range786500
display id: range786500
version: 1
direction: direction/forward
top level: Prm +
start: 9
end: 25
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation837284
display id: annotation837284
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
type: Range
persistent identity: range837284
display id: range837284
version: 1
direction: direction/forward
top level: Prm +
start: 48
end: 53
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2002
display id: BBa_R2002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Zif23 regulated, test: between and after
creator: Ronny Krashinsky
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is between the -10 and -35 sites, and a symmetric Zif23 dimer target site is after the -10 site.
mutable notes: consensus sequences based on [Jensen98].
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: Zif23
sequence annotation: consensus
sequence: https://synbiohub.org/public/igem/BBa_R2002_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation318163
display id: annotation318163
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318163/range318163/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318163/range318163/1
type: Range
persistent identity: range318163
display id: range318163
version: 1
direction: direction/forward
top level: Zif23
start: 33
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318160
display id: annotation318160
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318160/range318160/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318160/range318160/1
type: Range
persistent identity: range318160
display id: range318160
version: 1
direction: direction/forward
top level: Zif23
start: 10
end: 15
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318171
display id: annotation318171
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318171/range318171/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318171/range318171/1
type: Range
persistent identity: range318171
display id: range318171
version: 1
direction: direction/forward
top level: Zif23
start: 20
end: 33
orientation: inline

consensus
type: SequenceAnnotation
persistent identity: annotation318174
display id: annotation318174
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318174/range318174/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318174/range318174/1
type: Range
persistent identity: range318174
display id: range318174
version: 1
direction: direction/forward
top level: Zif23
start: 1
end: 9
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318164
display id: annotation318164
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318164/range318164/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318164/range318164/1
type: Range
persistent identity: range318164
display id: range318164
version: 1
direction: direction/forward
top level: Zif23
start: 39
end: 52
orientation: inline

BBa_J05209
type: ComponentDefinition
persistent identity: BBa J05209
display id: BBa_J05209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modifed Pr Promoter
creator: Alexander Roth
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is identical to BBa_R0051 exept that some base has been modifed to alow for additional ZFP repressors to bind
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: OR2
sequence annotation: ZFP binding sites
sequence: https://synbiohub.org/public/igem/BBa_J05209_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1689812
display id: annotation1689812
version: 1
top level: BBa_J05209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689812/range1689812/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689812/range1689812/1
type: Range
persistent identity: range1689812
display id: range1689812
version: 1
direction: direction/forward
top level: BBa_J05209
start: 38
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1689811
display id: annotation1689811
version: 1
top level: BBa_J05209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689811/range1689811/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689811/range1689811/1
type: Range
persistent identity: range1689811
display id: range1689811
version: 1
direction: direction/forward
top level: BBa_J05209
start: 15
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1689810
display id: annotation1689810
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689810/range1689810/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689810/range1689810/1
type: Range
persistent identity: range1689810
display id: range1689810
version: 1
direction: direction/forward
top level: BBa_J05209
start: 25
end: 41
orientation: inline

ZFP binding sites
type: SequenceAnnotation
persistent identity: annotation1689899
display id: annotation1689899
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689899/range1689899/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689899/range1689899/1
type: Range
persistent identity: range1689899
display id: range1689899
version: 1
direction: direction/forward
top level: BBa_J05209
start: 18
end: 29
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1689809
display id: annotation1689809
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689809/range1689809/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689809/range1689809/1
type: Range
persistent identity: range1689809
display id: range1689809
version: 1
direction: direction/forward
top level: BBa_J05209
start: 1
end: 17
orientation: inline

PenI
type: ComponentDefinition
persistent identity: BBa R0074
display id: BBa_R0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (PenI regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
mutable notes: extends slightly past -50
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: dimer left half
sequence annotation: -35
sequence annotation: dimer right half
sequence annotation: -10
sequence annotation: PenI
sequence: https://synbiohub.org/public/igem/BBa_R0074_sequence/1

dimer left half
type: SequenceAnnotation
persistent identity: annotation319788
display id: annotation319788
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
type: Range
persistent identity: range319788
display id: range319788
version: 1
direction: direction/forward
top level: PenI
start: 31
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319211
display id: annotation319211
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
type: Range
persistent identity: range319211
display id: range319211
version: 1
direction: direction/forward
top level: PenI
start: 23
end: 28
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation319789
display id: annotation319789
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
type: Range
persistent identity: range319789
display id: range319789
version: 1
direction: direction/forward
top level: PenI
start: 55
end: 76
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319212
display id: annotation319212
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
type: Range
persistent identity: range319212
display id: range319212
version: 1
direction: direction/forward
top level: PenI
start: 46
end: 51
orientation: inline

PenI
type: SequenceAnnotation
persistent identity: annotation302713
display id: annotation302713
version: 1
top level: PenI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1

https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
type: Range
persistent identity: range302713
display id: range302713
version: 1
direction: direction/forward
top level: PenI
start: 1
end: 77
orientation: inline

BBa_K116001
type: ComponentDefinition
persistent identity: BBa K116001
display id: BBa_K116001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nhaA promoter, that can be regulated by pH and nhaR protein.
creator: Chih-Hsien Yang
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:32Z
discontinued: false
dominant: true
group u list: _210_
m user id: 0
owner id: 592
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It's E.coli K12 sodium/proton anti-transport promoter. It can be a pH sensor. We test pH senses in different pH value and Sodium concentration.
mutable notes: It should contain NhaR binding site.
mutable provenance: E.coli K12 MG1655 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K116001_sequence/1

Pnot(O77)
type: ComponentDefinition
persistent identity: BBa I732207
display id: BBa_I732207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O77)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732207_sequence/1

BBa_K950000
type: ComponentDefinition
persistent identity: BBa K950000
display id: BBa_K950000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast fet3 promotor
creator: Jakob Matthes
created: 2012-07-09T11:00:00Z
modified: 2015-09-25T08:36:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1218_
m user id: 26366
owner id: 11576
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: promotor of the fet3 gene from S. cerevisiae, fet3 is repressed on high iron levels.
mutable notes: length of promoter was chosen to include binding sites and to obtain good primers
mutable provenance: genomic upstream sequence of fet3 gene from the Saccharomyces Genome Database (http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=fet3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: binding site
sequence: https://synbiohub.org/public/igem/BBa_K950000_sequence/1

binding site
type: SequenceAnnotation
persistent identity: annotation2182746
display id: annotation2182746
version: 1
top level: BBa_K950000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K950000/annotation2182746/range2182746/1

https://synbiohub.org/public/igem/BBa_K950000/annotation2182746/range2182746/1
type: Range
persistent identity: range2182746
display id: range2182746
version: 1
direction: direction/forward
top level: BBa_K950000
start: 264
end: 268
orientation: inline

BBa_K112401
type: ComponentDefinition
persistent identity: BBa K112401
display id: BBa_K112401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for recA gene - SOS and Ultrasound Sensitive
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the recA gene, a known SOS response gene, is upregulated 4 fold when cells are exposed to high frequency ultrasound and microbubble cavitation.
mutable notes: We did not see the desired response from ultrasound exposure that we expected from this promoter. However, we did not induce the same acoustic cavitation that Vollmer et. al. used to elicit a response from this promoter.
mutable provenance: Cloned from E. coli MG1655 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112401_sequence/1

BBa_R2114
type: ComponentDefinition
persistent identity: BBa R2114
display id: BBa_R2114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-22T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This part was inadvertently made while trying to make BBa_R2111. It contains a single base pair mutation in the -10 site of BBa_R2111.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 site
sequence annotation: transcription start site
sequence annotation: C2003 operator site
sequence annotation: -35 site
sequence: https://synbiohub.org/public/igem/BBa_R2114_sequence/1

-10 site
type: SequenceAnnotation
persistent identity: annotation1904659
display id: annotation1904659
version: 1
top level: BBa_R2114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904659/range1904659/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904659/range1904659/1
type: Range
persistent identity: range1904659
display id: range1904659
version: 1
direction: direction/forward
top level: BBa_R2114
start: 40
end: 45
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1904661
display id: annotation1904661
version: 1
top level: BBa_R2114
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904661/range1904661/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904661/range1904661/1
type: Range
persistent identity: range1904661
display id: range1904661
version: 1
direction: direction/forward
top level: BBa_R2114
start: 53
end: 53
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1904662
display id: annotation1904662
version: 1
top level: BBa_R2114
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904662/range1904662/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904662/range1904662/1
type: Range
persistent identity: range1904662
display id: range1904662
version: 1
direction: direction/forward
top level: BBa_R2114
start: 23
end: 34
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1904660
display id: annotation1904660
version: 1
top level: BBa_R2114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904660/range1904660/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904660/range1904660/1
type: Range
persistent identity: range1904660
display id: range1904660
version: 1
direction: direction/forward
top level: BBa_R2114
start: 17
end: 22
orientation: inline

BBa_K266006
type: ComponentDefinition
persistent identity: BBa K266006
display id: BBa_K266006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI+GFP & AI+LuxR --| LasI+GFP
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator
mutable notes: None.
mutable provenance: The biobricks BBa_K266005 and BBa_E0840 where used to build this biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K266005
component: BBa_E0840
sequence annotation: BBa_K266005
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K266006_sequence/1

BBa_K266005
type: Component
persistent identity: component2267607
display id: component2267607
version: 1
top level: BBa_K266006
access: public
definition: BBa_K266005

BBa_E0840
type: Component
persistent identity: component2267618
display id: component2267618
version: 1
top level: BBa_K266006
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_K266005
type: SequenceAnnotation
persistent identity: annotation2267607
display id: annotation2267607
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
component: BBa_K266005

https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
type: Range
persistent identity: range2267607
display id: range2267607
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 1
end: 819
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2267618
display id: annotation2267618
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
type: Range
persistent identity: range2267618
display id: range2267618
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 828
end: 1705
orientation: inline

BBa_M31370
type: ComponentDefinition
persistent identity: BBa M31370
display id: BBa_M31370
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tacI Promoter
creator: Michael Oh
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1395
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Allows for regulation of following gene with laqIq and IPTG...laqIq in same plasmid represses the promoter and IPTG proportionally depresses the promoter (Boer, et al., 1983).
mutable notes: Took sequence from Boer, 1983 paper.
mutable provenance: Derived from trp and lac promoter sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M31370_sequence/1

BBa_R0186
type: ComponentDefinition
persistent identity: BBa R0186
display id: BBa_R0186
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an A, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_R0186_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1962095
display id: annotation1962095
version: 1
top level: BBa_R0186
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0186/annotation1962095/range1962095/1

https://synbiohub.org/public/igem/BBa_R0186/annotation1962095/range1962095/1
type: Range
persistent identity: range1962095
display id: range1962095
version: 1
direction: direction/forward
top level: BBa_R0186
start: 1
end: 20
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962094
display id: annotation1962094
version: 1
top level: BBa_R0186
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0186/annotation1962094/range1962094/1

https://synbiohub.org/public/igem/BBa_R0186/annotation1962094/range1962094/1
type: Range
persistent identity: range1962094
display id: range1962094
version: 1
direction: direction/forward
top level: BBa_R0186
start: 20
end: 44
orientation: inline

BBa_I766200
type: ComponentDefinition
persistent identity: BBa I766200
display id: BBa_I766200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSte2
creator: Nili Sommovilla
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: endogenous promoter of Ste2
mutable notes: none.
mutable provenance: from yeast genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766200_sequence/1

NOR2
type: ComponentDefinition
persistent identity: BBa I732352
display id: BBa_I732352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOR Candidate (U035O44D001O22)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O61
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732352_sequence/1

BBa_K332031
type: ComponentDefinition
persistent identity: BBa K332031
display id: BBa_K332031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter + 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???. We speculate that when the ???Regulator??? and ???Production??? sections are combined, we can achieve a high bacteria growth rate and close to no target protein producing rate at T>37??C, and low bacteria growth rate and high target protein producing rate at T<37??C.
mutable notes: To regulate following gene by temperature
mutable provenance: iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K115002
component: BBa_C0040
component: BBa_J61048
component: BBa_J23101
sequence annotation: BBa_J61048
sequence annotation: BBa_K115002
sequence annotation: BBa_C0040
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K332031_sequence/1

BBa_K115002
type: Component
persistent identity: component2250402
display id: component2250402
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_C0040
type: Component
persistent identity: component2250406
display id: component2250406
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_J61048
type: Component
persistent identity: component2250407
display id: component2250407
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_J23101
type: Component
persistent identity: component2250397
display id: component2250397
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2250407
display id: annotation2250407
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
type: Range
persistent identity: range2250407
display id: range2250407
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 795
end: 907
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2250402
display id: annotation2250402
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
type: Range
persistent identity: range2250402
display id: range2250402
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 44
end: 95
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2250406
display id: annotation2250406
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
type: Range
persistent identity: range2250406
display id: range2250406
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 102
end: 786
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2250397
display id: annotation2250397
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
type: Range
persistent identity: range2250397
display id: range2250397
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 1
end: 35
orientation: inline

BBa_K086026
type: ComponentDefinition
persistent identity: BBa K086026
display id: BBa_K086026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_1
sequence: https://synbiohub.org/public/igem/BBa_K086026_sequence/1

P32_1
type: SequenceAnnotation
persistent identity: annotation1995760
display id: annotation1995760
version: 1
top level: BBa_K086026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086026/annotation1995760/range1995760/1

https://synbiohub.org/public/igem/BBa_K086026/annotation1995760/range1995760/1
type: Range
persistent identity: range1995760
display id: range1995760
version: 1
direction: direction/forward
top level: BBa_K086026
start: 1
end: 55
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732443
display id: BBa_I732443
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732443_sequence/1

pLacIQ1
type: ComponentDefinition
persistent identity: BBa K091112
display id: BBa_K091112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacIQ1 promoter
creator: Pallavi Penumetcha
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: this is a modified version of the LacIQ promoter that should bind LacIQ tighter than the original.
mutable notes: no special consideration
mutable provenance: This is a modified version of the LacIQ promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091112_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1964078
display id: annotation1964078
version: 1
top level: pLacIQ1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091112/annotation1964078/range1964078/1

https://synbiohub.org/public/igem/BBa_K091112/annotation1964078/range1964078/1
type: Range
persistent identity: range1964078
display id: range1964078
version: 1
direction: direction/forward
top level: pLacIQ1
start: 37
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1964077
display id: annotation1964077
version: 1
top level: pLacIQ1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091112/annotation1964077/range1964077/1

https://synbiohub.org/public/igem/BBa_K091112/annotation1964077/range1964077/1
type: Range
persistent identity: range1964077
display id: range1964077
version: 1
direction: direction/forward
top level: pLacIQ1
start: 12
end: 17
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732410
display id: BBa_I732410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D038O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O2
sequence annotation: consensus elements
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_I732410_sequence/1

O2
type: SequenceAnnotation
persistent identity: annotation1944559
display id: annotation1944559
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944559/range1944559/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944559/range1944559/1
type: Range
persistent identity: range1944559
display id: range1944559
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 134
end: 153
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944558
display id: annotation1944558
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944558/range1944558/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944558/range1944558/1
type: Range
persistent identity: range1944558
display id: range1944558
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 67
end: 137
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944560
display id: annotation1944560
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944560/range1944560/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944560/range1944560/1
type: Range
persistent identity: range1944560
display id: range1944560
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

BBa_K112405
type: ComponentDefinition
persistent identity: BBa K112405
display id: BBa_K112405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for CadA and CadB genes
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our microarray experiment showed that this gene was responsive to sound and ultrasound. Therefore, we cloned the promoter region located approximately 250 base pairs before the start codon for the cadB gene.
mutable notes: There was a relatively high basal level of expression of this gene that made this part difficult to incorporate into our lysis device design.
mutable provenance: cloned from E. coli MG1655 genome located upstream of CadA and CadB genes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112405_sequence/1

BBa_K093008
type: ComponentDefinition
persistent identity: BBa K093008
display id: BBa_K093008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: reverse BBa_R0011
creator: John Heil
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: reverse compliments of BBa_R0011
mutable notes: for downstream application
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacO1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K093008_sequence/1

lacO1
type: SequenceAnnotation
persistent identity: annotation1992543
display id: annotation1992543
version: 1
top level: BBa_K093008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992543/range1992543/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992543/range1992543/1
type: Range
persistent identity: range1992543
display id: range1992543
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 15
end: 29
orientation: inline

lacO1
type: SequenceAnnotation
persistent identity: annotation1992542
display id: annotation1992542
version: 1
top level: BBa_K093008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992542/range1992542/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992542/range1992542/1
type: Range
persistent identity: range1992542
display id: range1992542
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 36
end: 52
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1992541
display id: annotation1992541
version: 1
top level: BBa_K093008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992541/range1992541/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992541/range1992541/1
type: Range
persistent identity: range1992541
display id: range1992541
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 30
end: 35
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1992540
display id: annotation1992540
version: 1
top level: BBa_K093008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992540/range1992540/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992540/range1992540/1
type: Range
persistent identity: range1992540
display id: range1992540
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 8
end: 14
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lacI regulated, lambda pL hybrid)
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: lac O1
sequence annotation: BBa_R0011
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
direction: direction/forward
top level: lacI+pL
start: 43
end: 48
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
direction: direction/forward
top level: lacI+pL
start: 3
end: 19
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
direction: direction/forward
top level: lacI+pL
start: 1
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
direction: direction/forward
top level: lacI+pL
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
direction: direction/forward
top level: lacI+pL
start: 26
end: 42
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732445
display id: BBa_I732445
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732445_sequence/1

cII p22
type: ComponentDefinition
persistent identity: BBa R1053
display id: BBa_R1053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (p22 cII regulated)
creator: Maia Mahoney, Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: [Note: This is the same part as R0053 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
mutable notes: Modified from wild-type to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: -10
sequence annotation: OR3
sequence annotation: BBa_R1053
sequence: https://synbiohub.org/public/igem/BBa_R1053_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2094
display id: annotation2094
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2094/range2094/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2094/range2094/1
type: Range
persistent identity: range2094
display id: range2094
version: 1
direction: direction/forward
top level: cII p22
start: 8
end: 14
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2089
display id: annotation2089
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2089/range2089/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2089/range2089/1
type: Range
persistent identity: range2089
display id: range2089
version: 1
direction: direction/forward
top level: cII p22
start: 11
end: 29
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2090
display id: annotation2090
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2090/range2090/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2090/range2090/1
type: Range
persistent identity: range2090
display id: range2090
version: 1
direction: direction/forward
top level: cII p22
start: 35
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2095
display id: annotation2095
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2095/range2095/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2095/range2095/1
type: Range
persistent identity: range2095
display id: range2095
version: 1
direction: direction/forward
top level: cII p22
start: 31
end: 36
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2091
display id: annotation2091
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2091/range2091/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2091/range2091/1
type: Range
persistent identity: range2091
display id: range2091
version: 1
direction: direction/forward
top level: cII p22
start: 19
end: 24
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2088
display id: annotation2088
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2088/range2088/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2088/range2088/1
type: Range
persistent identity: range2088
display id: range2088
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 3
orientation: inline

BBa_R1053
type: SequenceAnnotation
persistent identity: annotation7076
display id: annotation7076
version: 1
top level: cII p22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1053/annotation7076/range7076/1

https://synbiohub.org/public/igem/BBa_R1053/annotation7076/range7076/1
type: Range
persistent identity: range7076
display id: range7076
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 55
orientation: inline

BBa_K091100
type: ComponentDefinition
persistent identity: BBa K091100
display id: BBa_K091100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac_lux hybrid promoter
creator: Pallavi Penumetcha
created: 2008-05-28T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 2993
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter is repressed by lacI and luxR with HSL. It contains a lux box and a lacI binding site.
mutable notes: Deleted the CAP binding site in R0010.
mutable provenance: R0010 modified with the insertion of a lux box in between -35 and -10.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lux box
sequence annotation: -10
sequence annotation: -35
sequence annotation: lacI binding site
sequence: https://synbiohub.org/public/igem/BBa_K091100_sequence/1

lux box
type: SequenceAnnotation
persistent identity: annotation1963335
display id: annotation1963335
version: 1
top level: BBa_K091100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1
type: Range
persistent identity: range1963335
display id: range1963335
version: 1
direction: direction/forward
top level: BBa_K091100
start: 16
end: 33
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963334
display id: annotation1963334
version: 1
top level: BBa_K091100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1
type: Range
persistent identity: range1963334
display id: range1963334
version: 1
direction: direction/forward
top level: BBa_K091100
start: 34
end: 39
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1963333
display id: annotation1963333
version: 1
top level: BBa_K091100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1
type: Range
persistent identity: range1963333
display id: range1963333
version: 1
direction: direction/forward
top level: BBa_K091100
start: 10
end: 15
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1963336
display id: annotation1963336
version: 1
top level: BBa_K091100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1
type: Range
persistent identity: range1963336
display id: range1963336
version: 1
direction: direction/forward
top level: BBa_K091100
start: 40
end: 74
orientation: inline

BBa_K921000
type: ComponentDefinition
persistent identity: BBa K921000
display id: BBa_K921000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant I)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: Initiation
sequence annotation: T7 promoter
sequence annotation: LacO
sequence: https://synbiohub.org/public/igem/BBa_K921000_sequence/1

Initiation
type: SequenceAnnotation
persistent identity: annotation2205291
display id: annotation2205291
version: 1
top level: BBa_K921000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1
type: Range
persistent identity: range2205291
display id: range2205291
version: 1
direction: direction/forward
top level: BBa_K921000
start: 19
end: 20
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2205289
display id: annotation2205289
version: 1
top level: BBa_K921000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1
type: Range
persistent identity: range2205289
display id: range2205289
version: 1
direction: direction/forward
top level: BBa_K921000
start: 1
end: 19
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205290
display id: annotation2205290
version: 1
top level: BBa_K921000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1
type: Range
persistent identity: range2205290
display id: range2205290
version: 1
direction: direction/forward
top level: BBa_K921000
start: 19
end: 46
orientation: inline

BBa_K086025
type: ComponentDefinition
persistent identity: BBa K086025
display id: BBa_K086025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: cleaned
mutable provenance: Lac O and sigma 28 binding sites' conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P28_4
sequence: https://synbiohub.org/public/igem/BBa_K086025_sequence/1

P28_4
type: SequenceAnnotation
persistent identity: annotation1995759
display id: annotation1995759
version: 1
top level: BBa_K086025
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086025/annotation1995759/range1995759/1

https://synbiohub.org/public/igem/BBa_K086025/annotation1995759/range1995759/1
type: Range
persistent identity: range1995759
display id: range1995759
version: 1
direction: direction/forward
top level: BBa_K086025
start: 1
end: 55
orientation: inline

BBa_I751502
type: ComponentDefinition
persistent identity: BBa I751502
display id: BBa_I751502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-lac hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: plac
sequence annotation: -10
sequence annotation: O1
sequence annotation: luxR binding site
sequence: https://synbiohub.org/public/igem/BBa_I751502_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1955560
display id: annotation1955560
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1
type: Range
persistent identity: range1955560
display id: range1955560
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 26
orientation: inline

plac
type: SequenceAnnotation
persistent identity: annotation1955559
display id: annotation1955559
version: 1
top level: BBa_I751502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1
type: Range
persistent identity: range1955559
display id: range1955559
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 74
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955561
display id: annotation1955561
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1
type: Range
persistent identity: range1955561
display id: range1955561
version: 1
direction: direction/forward
top level: BBa_I751502
start: 42
end: 48
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955562
display id: annotation1955562
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1
type: Range
persistent identity: range1955562
display id: range1955562
version: 1
direction: direction/forward
top level: BBa_I751502
start: 27
end: 41
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955563
display id: annotation1955563
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1
type: Range
persistent identity: range1955563
display id: range1955563
version: 1
direction: direction/forward
top level: BBa_I751502
start: 49
end: 74
orientation: inline

luxR binding site
type: SequenceAnnotation
persistent identity: annotation1955564
display id: annotation1955564
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1
type: Range
persistent identity: range1955564
display id: range1955564
version: 1
direction: direction/forward
top level: BBa_I751502
start: 1
end: 19
orientation: inline

BBa_I756015
type: ComponentDefinition
persistent identity: BBa I756015
display id: BBa_I756015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV Promoter with lac operator sites
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CMV Promoter with lac operator sites.
mutable notes: CMV promoter with inserted lac operator sites. Lac operator from POPI3Cat
mutable provenance: Promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lac operator
sequence: https://synbiohub.org/public/igem/BBa_I756015_sequence/1

Lac operator
type: SequenceAnnotation
persistent identity: annotation1941580
display id: annotation1941580
version: 1
top level: BBa_I756015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I756015/annotation1941580/range1941580/1

https://synbiohub.org/public/igem/BBa_I756015/annotation1941580/range1941580/1
type: Range
persistent identity: range1941580
display id: range1941580
version: 1
direction: direction/forward
top level: BBa_I756015
start: 513
end: 619
orientation: inline

BBa_I12040
type: ComponentDefinition
persistent identity: BBa I12040
display id: BBa_I12040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lambda P(RM) promoter: -10 region from P(L) and cooperatively repressed by 434 cI
creator: ryhsiao
created: 2004-08-18T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 148
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba P(RM) promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operator sites are used for both activation and repression to enhance cooperativity. Additionally, the -10 region from lambda P(L) was used instead of that from P(RM).
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage. This part differs from BBa_I12036 because the -10 region from P(L) was used instead of that from P(RM).
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40. ; Lutz, R & Bujard, H. "Independent and tight regulation of transcriptional units in Escherichia coli via the lacR/O, the TetR/O and AraC/I1-I2 regulatory elements, Nucleic Acids Research, 1997, Vol.25, No.6 (1203-1210)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2 434
sequence annotation: start
sequence annotation: -10
sequence annotation: -35
sequence annotation: OR1 434
sequence annotation: OR2 lambda
sequence annotation: OR1 lambda
sequence: https://synbiohub.org/public/igem/BBa_I12040_sequence/1

OR2 434
type: SequenceAnnotation
persistent identity: annotation1059303
display id: annotation1059303
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059303/range1059303/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059303/range1059303/1
type: Range
persistent identity: range1059303
display id: range1059303
version: 1
direction: direction/forward
top level: BBa_I12040
start: 78
end: 91
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1062263
display id: annotation1062263
version: 1
top level: BBa_I12040
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1062263/range1062263/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1062263/range1062263/1
type: Range
persistent identity: range1062263
display id: range1062263
version: 1
direction: direction/forward
top level: BBa_I12040
start: 83
end: 83
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1059302
display id: annotation1059302
version: 1
top level: BBa_I12040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059302/range1059302/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059302/range1059302/1
type: Range
persistent identity: range1059302
display id: range1059302
version: 1
direction: direction/forward
top level: BBa_I12040
start: 71
end: 76
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1059300
display id: annotation1059300
version: 1
top level: BBa_I12040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059300/range1059300/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059300/range1059300/1
type: Range
persistent identity: range1059300
display id: range1059300
version: 1
direction: direction/forward
top level: BBa_I12040
start: 48
end: 53
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation1059301
display id: annotation1059301
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059301/range1059301/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059301/range1059301/1
type: Range
persistent identity: range1059301
display id: range1059301
version: 1
direction: direction/forward
top level: BBa_I12040
start: 56
end: 69
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1059299
display id: annotation1059299
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059299/range1059299/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059299/range1059299/1
type: Range
persistent identity: range1059299
display id: range1059299
version: 1
direction: direction/forward
top level: BBa_I12040
start: 33
end: 49
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1059298
display id: annotation1059298
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059298/range1059298/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059298/range1059298/1
type: Range
persistent identity: range1059298
display id: range1059298
version: 1
direction: direction/forward
top level: BBa_I12040
start: 9
end: 25
orientation: inline

BBa_J64069
type: ComponentDefinition
persistent identity: BBa J64069
display id: BBa_J64069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0065 with lux box deleted
creator: Jeffrey J Tabor
created: 2008-02-10T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: luxR operator (lux box) was deleted from R0065 and replaced with a BamHI hexamer.
mutable notes: added a bamHI site immediately upstream of -35 in place of lux operator
mutable provenance: constructed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: +1
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J64069_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1961161
display id: annotation1961161
version: 1
top level: BBa_J64069
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64069/annotation1961161/range1961161/1

https://synbiohub.org/public/igem/BBa_J64069/annotation1961161/range1961161/1
type: Range
persistent identity: range1961161
display id: range1961161
version: 1
direction: direction/forward
top level: BBa_J64069
start: 34
end: 39
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1961162
display id: annotation1961162
version: 1
top level: BBa_J64069
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64069/annotation1961162/range1961162/1

https://synbiohub.org/public/igem/BBa_J64069/annotation1961162/range1961162/1
type: Range
persistent identity: range1961162
display id: range1961162
version: 1
direction: direction/forward
top level: BBa_J64069
start: 46
end: 46
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961160
display id: annotation1961160
version: 1
top level: BBa_J64069
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64069/annotation1961160/range1961160/1

https://synbiohub.org/public/igem/BBa_J64069/annotation1961160/range1961160/1
type: Range
persistent identity: range1961160
display id: range1961160
version: 1
direction: direction/forward
top level: BBa_J64069
start: 12
end: 17
orientation: inline

BBa_S04509
type: ComponentDefinition
persistent identity: BBa S04509
display id: BBa_S04509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010:B0034
creator: Jacky, Fong Chuen, Loo
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:14:41Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 6123
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_S04509_sequence/1

BBa_B0034
type: Component
persistent identity: component2090743
display id: component2090743
version: 1
top level: BBa_S04509
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component2090735
display id: component2090735
version: 1
top level: BBa_S04509
access: public
definition: LacI

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2090743
display id: annotation2090743
version: 1
top level: BBa_S04509
location: https://synbiohub.org/public/igem/BBa_S04509/annotation2090743/range2090743/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04509/annotation2090743/range2090743/1
type: Range
persistent identity: range2090743
display id: range2090743
version: 1
direction: direction/reverse
top level: BBa_S04509
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2090735
display id: annotation2090735
version: 1
top level: BBa_S04509
location: https://synbiohub.org/public/igem/BBa_S04509/annotation2090735/range2090735/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_S04509/annotation2090735/range2090735/1
type: Range
persistent identity: range2090735
display id: range2090735
version: 1
direction: direction/forward
top level: BBa_S04509
start: 1
end: 200
orientation: inline

BBa_K1833001
type: ComponentDefinition
persistent identity: BBa K1833001
display id: BBa_K1833001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pProt-GFP
creator: Konstantin Borisov
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T08:02:07Z
discontinued: false
dominant: false
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part produces GFP in the presence of sigma-70 E. coli RNA polymerase; however, the promoter contains two sites for a synthetic repressor to bind. Without the presence of this repressor, the part can serve as a strong constitutive promoter, as shown in the pictures below. For more information on the repressor, visit http://2015.igem.org/Team:Pitt/Protease/Project. The GFP produced is a mutant known as GFPmut3b, and the original citation is as follows: Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). (http://www.sciencedirect.com/science/article/pii/0378111995006850) It has a maximum excitation at 501 nm and maximum emission at 511 nm. For more information, see part BBa_E0040.
mutable notes: The part containing GFP was cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part. Note that due to the choice of technique, a Biobrick scar site remains between the promoter and RBS.
mutable provenance: The RBS-GFP-terminator was obtained from as part BBa_E0840 from the 2015 Distribution (Plate 2, Well 24D). The pProt promoter was obtained as two short oligonucleotides annealed to form sticky ends. The part containing GFP was then cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0840
component: BBa_K1833998
sequence annotation: BBa_E0840
sequence annotation: BBa_K1833998
sequence: https://synbiohub.org/public/igem/BBa_K1833001_sequence/1

BBa_E0840
type: Component
persistent identity: component2460170
display id: component2460170
version: 1
top level: BBa_K1833001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_K1833998
type: Component
persistent identity: component2460159
display id: component2460159
version: 1
top level: BBa_K1833001
access: public
definition: BBa_K1833998

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2460170
display id: annotation2460170
version: 1
top level: BBa_K1833001
location: https://synbiohub.org/public/igem/BBa_K1833001/annotation2460170/range2460170/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1833001/annotation2460170/range2460170/1
type: Range
persistent identity: range2460170
display id: range2460170
version: 1
direction: direction/reverse
top level: BBa_K1833001
start: 54
end: 931
orientation: inline

BBa_K1833998
type: SequenceAnnotation
persistent identity: annotation2460159
display id: annotation2460159
version: 1
top level: BBa_K1833001
location: https://synbiohub.org/public/igem/BBa_K1833001/annotation2460159/range2460159/1
component: BBa_K1833998

https://synbiohub.org/public/igem/BBa_K1833001/annotation2460159/range2460159/1
type: Range
persistent identity: range2460159
display id: range2460159
version: 1
direction: direction/reverse
top level: BBa_K1833001
start: 1
end: 53
orientation: inline

BBa_K137047
type: ComponentDefinition
persistent identity: BBa K137047
display id: BBa_K137047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 250 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA downstream of it to make the total length 250 bp. The promoter faces the 5' direction.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137047_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968020
display id: annotation1968020
version: 1
top level: BBa_K137047
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137047/annotation1968020/range1968020/1

https://synbiohub.org/public/igem/BBa_K137047/annotation1968020/range1968020/1
type: Range
persistent identity: range1968020
display id: range1968020
version: 1
direction: direction/reverse
top level: BBa_K137047
start: 1
end: 54
orientation: inline

BBa_I756016
type: ComponentDefinition
persistent identity: BBa I756016
display id: BBa_I756016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV-tet promoter
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CMV promoter with tet operator
mutable notes: CMV promoter with tet operator
mutable provenance: CMV promoter with tet operator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tet operator
sequence: https://synbiohub.org/public/igem/BBa_I756016_sequence/1

tet operator
type: SequenceAnnotation
persistent identity: annotation1941581
display id: annotation1941581
version: 1
top level: BBa_I756016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I756016/annotation1941581/range1941581/1

https://synbiohub.org/public/igem/BBa_I756016/annotation1941581/range1941581/1
type: Range
persistent identity: range1941581
display id: range1941581
version: 1
direction: direction/forward
top level: BBa_I756016
start: 525
end: 578
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa R0052
display id: BBa_R0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (434 cI regulated)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: -35
sequence annotation: OR2
sequence annotation: start
sequence annotation: -10
sequence annotation: BBa_R0052
sequence: https://synbiohub.org/public/igem/BBa_R0052_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2028
display id: annotation2028
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
type: Range
persistent identity: range2028
display id: range2028
version: 1
direction: direction/forward
top level: cI 434
start: 30
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2029
display id: annotation2029
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
type: Range
persistent identity: range2029
display id: range2029
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 6
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2026
display id: annotation2026
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
type: Range
persistent identity: range2026
display id: range2026
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2031
display id: annotation2031
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
type: Range
persistent identity: range2031
display id: range2031
version: 1
direction: direction/forward
top level: cI 434
start: 2
end: 7
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2027
display id: annotation2027
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
type: Range
persistent identity: range2027
display id: range2027
version: 1
direction: direction/forward
top level: cI 434
start: 8
end: 21
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2032
display id: annotation2032
version: 1
top level: cI 434
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
type: Range
persistent identity: range2032
display id: range2032
version: 1
direction: direction/forward
top level: cI 434
start: 35
end: 37
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2030
display id: annotation2030
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
type: Range
persistent identity: range2030
display id: range2030
version: 1
direction: direction/forward
top level: cI 434
start: 24
end: 29
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation7068
display id: annotation7068
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1

https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
type: Range
persistent identity: range7068
display id: range7068
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 46
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732420
display id: BBa_I732420
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732420_sequence/1

pArsR
type: ComponentDefinition
persistent identity: BBa K190015
display id: BBa_K190015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arsenic Promoter (ArsR regulated)
creator: Michael Verhoeven
created: 2009-07-14T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _306_
m user id: 0
owner id: 4144
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Promoter sequence with recognition site for ArsR transcriptional regulator protein. ArsR binds to the promoter sequence in the absence of As and releases on binding of As, therby activating transcription.
mutable notes: None
mutable provenance: E.coli TOP10
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 and -10 region
sequence annotation: ArsR binding site
sequence: https://synbiohub.org/public/igem/BBa_K190015_sequence/1

-35 and -10 region
type: SequenceAnnotation
persistent identity: annotation2011502
display id: annotation2011502
version: 1
top level: pArsR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K190015/annotation2011502/range2011502/1

https://synbiohub.org/public/igem/BBa_K190015/annotation2011502/range2011502/1
type: Range
persistent identity: range2011502
display id: range2011502
version: 1
direction: direction/forward
top level: pArsR
start: 38
end: 72
orientation: inline

ArsR binding site
type: SequenceAnnotation
persistent identity: annotation2011501
display id: annotation2011501
version: 1
top level: pArsR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K190015/annotation2011501/range2011501/1

https://synbiohub.org/public/igem/BBa_K190015/annotation2011501/range2011501/1
type: Range
persistent identity: range2011501
display id: range2011501
version: 1
direction: direction/forward
top level: pArsR
start: 9
end: 41
orientation: inline

BBa_J54016
type: ComponentDefinition
persistent identity: BBa J54016
display id: BBa_J54016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter_lacq
creator: Yusaku Nakashima
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:48:11Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: lacq promoter
mutable notes: As Promoter
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: promoter_lacq
sequence: https://synbiohub.org/public/igem/BBa_J54016_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1903168
display id: annotation1903168
version: 1
top level: BBa_J54016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54016/annotation1903168/range1903168/1

https://synbiohub.org/public/igem/BBa_J54016/annotation1903168/range1903168/1
type: Range
persistent identity: range1903168
display id: range1903168
version: 1
direction: direction/forward
top level: BBa_J54016
start: 12
end: 17
orientation: inline

promoter_lacq
type: SequenceAnnotation
persistent identity: annotation1903169
display id: annotation1903169
version: 1
top level: BBa_J54016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J54016/annotation1903169/range1903169/1

https://synbiohub.org/public/igem/BBa_J54016/annotation1903169/range1903169/1
type: Range
persistent identity: range1903169
display id: range1903169
version: 1
direction: direction/forward
top level: BBa_J54016
start: 18
end: 49
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732412
display id: BBa_I732412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D062O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus elements
sequence annotation: O2
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_I732412_sequence/1

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944564
display id: annotation1944564
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944564/range1944564/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944564/range1944564/1
type: Range
persistent identity: range1944564
display id: range1944564
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 61
end: 137
orientation: inline

O2
type: SequenceAnnotation
persistent identity: annotation1944565
display id: annotation1944565
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944565/range1944565/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944565/range1944565/1
type: Range
persistent identity: range1944565
display id: range1944565
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 158
end: 177
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944566
display id: annotation1944566
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944566/range1944566/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944566/range1944566/1
type: Range
persistent identity: range1944566
display id: range1944566
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

Pnot(O44)
type: ComponentDefinition
persistent identity: BBa I732204
display id: BBa_I732204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O44)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732204_sequence/1

Zif23
type: ComponentDefinition
persistent identity: BBa R2001
display id: BBa_R2001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Zif23 regulated, test: after
creator: Ronny Krashinsky
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is after the -10 site.
mutable notes: consensus sequences based on [Jensen98].
mutable provenance: synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: (+1)
sequence annotation: consensus
sequence annotation: Zif23
sequence: https://synbiohub.org/public/igem/BBa_R2001_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation318132
display id: annotation318132
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318132/range318132/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318132/range318132/1
type: Range
persistent identity: range318132
display id: range318132
version: 1
direction: direction/forward
top level: Zif23
start: 10
end: 15
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318133
display id: annotation318133
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318133/range318133/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318133/range318133/1
type: Range
persistent identity: range318133
display id: range318133
version: 1
direction: direction/forward
top level: Zif23
start: 33
end: 38
orientation: inline

(+1)
type: SequenceAnnotation
persistent identity: annotation318134
display id: annotation318134
version: 1
top level: Zif23
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318134/range318134/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318134/range318134/1
type: Range
persistent identity: range318134
display id: range318134
version: 1
direction: direction/forward
top level: Zif23
start: 45
end: 45
orientation: inline

consensus
type: SequenceAnnotation
persistent identity: annotation318135
display id: annotation318135
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318135/range318135/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318135/range318135/1
type: Range
persistent identity: range318135
display id: range318135
version: 1
direction: direction/forward
top level: Zif23
start: 1
end: 9
orientation: inline

consensus
type: SequenceAnnotation
persistent identity: annotation318142
display id: annotation318142
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318142/range318142/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318142/range318142/1
type: Range
persistent identity: range318142
display id: range318142
version: 1
direction: direction/forward
top level: Zif23
start: 30
end: 32
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318136
display id: annotation318136
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318136/range318136/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318136/range318136/1
type: Range
persistent identity: range318136
display id: range318136
version: 1
direction: direction/forward
top level: Zif23
start: 39
end: 52
orientation: inline

NAND01
type: ComponentDefinition
persistent identity: BBa I732303
display id: BBa_I732303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D001O46)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O11
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732303_sequence/1

BBa_K091146
type: ComponentDefinition
persistent identity: BBa K091146
display id: BBa_K091146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas/Lux Hybrid Promoter
creator: Erin Feeney
created: 2008-07-16T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: K091146 is a modified version of the pLas' promoter (BBa_K091117) containing the addition of a luxR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of PAI-1 and LasR and repressed in the presence of AI-1 and LuxR.
mutable notes: The Lux box was placed adjacent to the LasR binding site with the aim of minimizing cross-talk in the presence of both PAI-1 and AI-1; since the LasR site should have a higher affinity, we hope that the binding of PAI-1 will competitively exclude the binding of AI-1.
mutable provenance: The LuxR binding site is from BBa_R0062 and the pLas' promoter is from the region upstream of the LasI gene in Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR
sequence annotation: -10
sequence annotation: -35
sequence annotation: LasR
sequence annotation: Transcriptional Start
sequence: https://synbiohub.org/public/igem/BBa_K091146_sequence/1

LuxR
type: SequenceAnnotation
persistent identity: annotation1967355
display id: annotation1967355
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1
type: Range
persistent identity: range1967355
display id: range1967355
version: 1
direction: direction/forward
top level: BBa_K091146
start: 94
end: 113
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967356
display id: annotation1967356
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1
type: Range
persistent identity: range1967356
display id: range1967356
version: 1
direction: direction/forward
top level: BBa_K091146
start: 113
end: 118
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967357
display id: annotation1967357
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1
type: Range
persistent identity: range1967357
display id: range1967357
version: 1
direction: direction/forward
top level: BBa_K091146
start: 86
end: 91
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation1967354
display id: annotation1967354
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1
type: Range
persistent identity: range1967354
display id: range1967354
version: 1
direction: direction/forward
top level: BBa_K091146
start: 75
end: 93
orientation: inline

Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1967358
display id: annotation1967358
version: 1
top level: BBa_K091146
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1
type: Range
persistent identity: range1967358
display id: range1967358
version: 1
direction: direction/forward
top level: BBa_K091146
start: 125
end: 125
orientation: inline

BBa_I732451
type: ComponentDefinition
persistent identity: BBa I732451
display id: BBa_I732451
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O27+D062NUL)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732451_sequence/1

BBa_K1520509
type: ComponentDefinition
persistent identity: BBa K1520509
display id: BBa_K1520509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our suicide circuit for gold ions.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, tetR, PtetO, rfp, Plac, tetR, Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520506
component: BBa_K1520026
component: BBa_K1520029
component: BBa_K1520505
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520026
sequence annotation: BBa_K1520506
sequence annotation: BBa_K1520505
sequence: https://synbiohub.org/public/igem/BBa_K1520509_sequence/1

BBa_K1520506
type: Component
persistent identity: component2385527
display id: component2385527
version: 1
top level: BBa_K1520509
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520506/1

BBa_K1520026
type: Component
persistent identity: component2385559
display id: component2385559
version: 1
top level: BBa_K1520509
access: public
definition: BBa_K1520026

BBa_K1520029
type: Component
persistent identity: component2385531
display id: component2385531
version: 1
top level: BBa_K1520509
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520505
type: Component
persistent identity: component2385530
display id: component2385530
version: 1
top level: BBa_K1520509
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520505/1

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385531
display id: annotation2385531
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385531/range2385531/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385531/range2385531/1
type: Range
persistent identity: range2385531
display id: range2385531
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 1473
end: 1601
orientation: inline

BBa_K1520026
type: SequenceAnnotation
persistent identity: annotation2385559
display id: annotation2385559
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385559/range2385559/1
component: BBa_K1520026

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385559/range2385559/1
type: Range
persistent identity: range2385559
display id: range2385559
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 1610
end: 4955
orientation: inline

BBa_K1520506
type: SequenceAnnotation
persistent identity: annotation2385527
display id: annotation2385527
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385527/range2385527/1
component: BBa_K1520506

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385527/range2385527/1
type: Range
persistent identity: range2385527
display id: range2385527
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 1
end: 753
orientation: inline

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385530
display id: annotation2385530
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385530/range2385530/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385530/range2385530/1
type: Range
persistent identity: range2385530
display id: range2385530
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 762
end: 1464
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2000
display id: BBa_R2000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Zif23 regulated, test: between
creator: Ronny Krashinsky
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is between the -10 and -35 sites.
mutable notes: consensus sequences based on [Jensen98].
mutable provenance: synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus
sequence annotation: (+1)
sequence annotation: -10
sequence annotation: -35
sequence annotation: Zif23
sequence: https://synbiohub.org/public/igem/BBa_R2000_sequence/1

consensus
type: SequenceAnnotation
persistent identity: annotation318100
display id: annotation318100
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318100/range318100/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318100/range318100/1
type: Range
persistent identity: range318100
display id: range318100
version: 1
direction: direction/forward
top level: Zif23
start: 1
end: 9
orientation: inline

(+1)
type: SequenceAnnotation
persistent identity: annotation318102
display id: annotation318102
version: 1
top level: Zif23
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318102/range318102/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318102/range318102/1
type: Range
persistent identity: range318102
display id: range318102
version: 1
direction: direction/forward
top level: Zif23
start: 45
end: 45
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318098
display id: annotation318098
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318098/range318098/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318098/range318098/1
type: Range
persistent identity: range318098
display id: range318098
version: 1
direction: direction/forward
top level: Zif23
start: 33
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318094
display id: annotation318094
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318094/range318094/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318094/range318094/1
type: Range
persistent identity: range318094
display id: range318094
version: 1
direction: direction/forward
top level: Zif23
start: 10
end: 15
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318099
display id: annotation318099
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318099/range318099/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318099/range318099/1
type: Range
persistent identity: range318099
display id: range318099
version: 1
direction: direction/forward
top level: Zif23
start: 20
end: 33
orientation: inline

BBa_K1520026
type: ComponentDefinition
persistent identity: BBa K1520026
display id: BBa_K1520026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter. It produces rfp when there is less lactose.
mutable notes: None
mutable provenance: Ptet, rfp, Plac, tetR, Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520025
component: BBa_K1520008
sequence annotation: BBa_K1520025
sequence annotation: BBa_K1520008
sequence: https://synbiohub.org/public/igem/BBa_K1520026_sequence/1

BBa_K1520025
type: Component
persistent identity: component2385313
display id: component2385313
version: 1
top level: BBa_K1520026
access: public
definition: BBa_K1520025

BBa_K1520008
type: Component
persistent identity: component2385319
display id: component2385319
version: 1
top level: BBa_K1520026
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520008/1

BBa_K1520025
type: SequenceAnnotation
persistent identity: annotation2385313
display id: annotation2385313
version: 1
top level: BBa_K1520026
location: https://synbiohub.org/public/igem/BBa_K1520026/annotation2385313/range2385313/1
component: BBa_K1520025

https://synbiohub.org/public/igem/BBa_K1520026/annotation2385313/range2385313/1
type: Range
persistent identity: range2385313
display id: range2385313
version: 1
direction: direction/reverse
top level: BBa_K1520026
start: 1
end: 1987
orientation: inline

BBa_K1520008
type: SequenceAnnotation
persistent identity: annotation2385319
display id: annotation2385319
version: 1
top level: BBa_K1520026
location: https://synbiohub.org/public/igem/BBa_K1520026/annotation2385319/range2385319/1
component: BBa_K1520008

https://synbiohub.org/public/igem/BBa_K1520026/annotation2385319/range2385319/1
type: Range
persistent identity: range2385319
display id: range2385319
version: 1
direction: direction/reverse
top level: BBa_K1520026
start: 1996
end: 3346
orientation: inline

Lux
type: ComponentDefinition
persistent identity: BBa I1051
display id: BBa_I1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux cassette right promoter
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.In thisversion, a binding site for cI dimer has been added: binding to this site dominantly represses transcription.
mutable provenance: V. fishceri and phage lambda (courtesy of Ron Weiss)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_I1051
sequence annotation: start
sequence annotation: -35
sequence annotation: -10
sequence annotation: cI (OR1)
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_I1051_sequence/1

BBa_I1051
type: SequenceAnnotation
persistent identity: annotation7059
display id: annotation7059
version: 1
top level: Lux
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1

https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1
type: Range
persistent identity: range7059
display id: range7059
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 68
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1940
display id: annotation1940
version: 1
top level: Lux
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1
type: Range
persistent identity: range1940
display id: range1940
version: 1
direction: direction/forward
top level: Lux
start: 53
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1937
display id: annotation1937
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1
type: Range
persistent identity: range1937
display id: range1937
version: 1
direction: direction/forward
top level: Lux
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1938
display id: annotation1938
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1
type: Range
persistent identity: range1938
display id: range1938
version: 1
direction: direction/forward
top level: Lux
start: 42
end: 47
orientation: inline

cI (OR1)
type: SequenceAnnotation
persistent identity: annotation1939
display id: annotation1939
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1
type: Range
persistent identity: range1939
display id: range1939
version: 1
direction: direction/forward
top level: Lux
start: 52
end: 68
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation1936
display id: annotation1936
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1
type: Range
persistent identity: range1936
display id: range1936
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 20
orientation: inline

BBa_K086029
type: ComponentDefinition
persistent identity: BBa K086029
display id: BBa_K086029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_4
sequence: https://synbiohub.org/public/igem/BBa_K086029_sequence/1

P32_4
type: SequenceAnnotation
persistent identity: annotation1995763
display id: annotation1995763
version: 1
top level: BBa_K086029
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086029/annotation1995763/range1995763/1

https://synbiohub.org/public/igem/BBa_K086029/annotation1995763/range1995763/1
type: Range
persistent identity: range1995763
display id: range1995763
version: 1
direction: direction/forward
top level: BBa_K086029
start: 1
end: 55
orientation: inline

BBa_K086023
type: ComponentDefinition
persistent identity: BBa K086023
display id: BBa_K086023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: cleaned
mutable provenance: Lac O and Sigma 28 binding sites' conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P28_2
sequence: https://synbiohub.org/public/igem/BBa_K086023_sequence/1

P28_2
type: SequenceAnnotation
persistent identity: annotation1995757
display id: annotation1995757
version: 1
top level: BBa_K086023
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086023/annotation1995757/range1995757/1

https://synbiohub.org/public/igem/BBa_K086023/annotation1995757/range1995757/1
type: Range
persistent identity: range1995757
display id: range1995757
version: 1
direction: direction/forward
top level: BBa_K086023
start: 1
end: 55
orientation: inline

Pnot(O26)
type: ComponentDefinition
persistent identity: BBa I732275
display id: BBa_I732275
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O26)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732275_sequence/1

BBa_J22106
type: ComponentDefinition
persistent identity: BBa J22106
display id: BBa_J22106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rec A (SOS) Promoter
creator: Krithiga Sankaran
created: 2006-09-04T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: true
group u list: _70_
m user id: 0
owner id: 1038
owning group id: 70
sample status: In stock
status: status/Available
bookmark: false
mutable description: Rec A (SOS) promoter.
mutable notes: NA
mutable provenance: The recA(SOS)promoter gene from the chromosomal DNA isolated from MG1655 has been PCR amplified and inserted into plasmid pSB1A2(part number BBa_B0015).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J22106_sequence/1

BBa_K873002
type: ComponentDefinition
persistent identity: BBa K873002
display id: BBa_K873002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HSP promoter
creator: yue hu
created: 2012-09-17T11:00:00Z
modified: 2016-10-16T09:49:24Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1134_
m user id: 29300
owner id: 11547
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a heat shock promoter, it refers to the transcription factor sigma32. when the temperature rise, the transcription factor sigma32 expresses a lot and binds to the RNA ploymerase to recognize the conserved sequence in -35 and -10 region, then the promoter starts to transcript.
mutable notes: The whole sequence of this part is only 90bp, so take a futher consideration if you intend to digest it for use.
mutable provenance: We align the HSP promoters from the literature and then find the conserved sequence, then we just modified the promoter of groES of E.coli, and synthesis the part through BIC genescript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K873002_sequence/1

LacI:Cpn10
type: ComponentDefinition
persistent identity: BBa K538200
display id: BBa_K538200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + Cpn10
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_K538000
component: BBa_B0034
sequence annotation: BBa_K538000
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K538200_sequence/1

BBa_R0010
type: Component
persistent identity: component2126718
display id: component2126718
version: 1
top level: LacI:Cpn10
access: public
definition: LacI

BBa_K538000
type: Component
persistent identity: component2126729
display id: component2126729
version: 1
top level: LacI:Cpn10
access: public
definition: https://synbiohub.org/public/igem/BBa_K538000/1

BBa_B0034
type: Component
persistent identity: component2126726
display id: component2126726
version: 1
top level: LacI:Cpn10
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538000
type: SequenceAnnotation
persistent identity: annotation2126729
display id: annotation2126729
version: 1
top level: LacI:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538200/annotation2126729/range2126729/1
component: BBa_K538000

https://synbiohub.org/public/igem/BBa_K538200/annotation2126729/range2126729/1
type: Range
persistent identity: range2126729
display id: range2126729
version: 1
direction: direction/reverse
top level: LacI:Cpn10
start: 227
end: 523
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2126726
display id: annotation2126726
version: 1
top level: LacI:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538200/annotation2126726/range2126726/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538200/annotation2126726/range2126726/1
type: Range
persistent identity: range2126726
display id: range2126726
version: 1
direction: direction/reverse
top level: LacI:Cpn10
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2126718
display id: annotation2126718
version: 1
top level: LacI:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538200/annotation2126718/range2126718/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538200/annotation2126718/range2126718/1
type: Range
persistent identity: range2126718
display id: range2126718
version: 1
direction: direction/forward
top level: LacI:Cpn10
start: 1
end: 200
orientation: inline

BBa_K950002
type: ComponentDefinition
persistent identity: BBa K950002
display id: BBa_K950002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast anb1 promotor
creator: Jakob Matthes
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1218_
m user id: 0
owner id: 11576
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the promotor of yeast anb1 gene. Panb1 is a target of the rox1 repressor.
mutable notes: genomic sequence was not altered
mutable provenance: sequence and information from http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=SUC2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: rox1 binding site
sequence: https://synbiohub.org/public/igem/BBa_K950002_sequence/1

rox1 binding site
type: SequenceAnnotation
persistent identity: annotation2179270
display id: annotation2179270
version: 1
top level: BBa_K950002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K950002/annotation2179270/range2179270/1

https://synbiohub.org/public/igem/BBa_K950002/annotation2179270/range2179270/1
type: Range
persistent identity: range2179270
display id: range2179270
version: 1
direction: direction/forward
top level: BBa_K950002
start: 186
end: 191
orientation: inline

rox1 binding site
type: SequenceAnnotation
persistent identity: annotation2179269
display id: annotation2179269
version: 1
top level: BBa_K950002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K950002/annotation2179269/range2179269/1

https://synbiohub.org/public/igem/BBa_K950002/annotation2179269/range2179269/1
type: Range
persistent identity: range2179269
display id: range2179269
version: 1
direction: direction/forward
top level: BBa_K950002
start: 88
end: 93
orientation: inline

BBa_K101002
type: ComponentDefinition
persistent identity: BBa K101002
display id: BBa_K101002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for p22 cII and TetR
creator: Bennett Swiniarski
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: k;kljg
mutable notes: TetR usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. However, p22 mnt also has two operator sites, one between -35 and -10 and another downstream from -10. Since each had an operator site in between -35 and -10, we had to eliminate the TetR operator site in order to allow the promoter to work. TetR was picked because it has a stronger binding than p22 mnt, and so would be more able to use only one site to bind.
mutable provenance: This part was designed by combining sequences from parts R0040 (TetR promoter) and R0053 (p22 cII promoter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101002_sequence/1

BBa_K145150
type: ComponentDefinition
persistent identity: BBa K145150
display id: BBa_K145150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter: HSL-LuxR activated, P22 C2 repressed
creator: Jonas Demeulemeester
created: 2008-08-05T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Hybrid promoter consisting of the Lux box which overlaps partly with -35 box. Binding sites for P22 C2 OR2 and OR1 are located between the -35 and -10 boxes and downstream of them respectively.
mutable notes: -35 box is partly composite. -10 is taken from the P22 phage PR regulatory region
mutable provenance: Synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: Lux-box
sequence: https://synbiohub.org/public/igem/BBa_K145150_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1971583
display id: annotation1971583
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1
type: Range
persistent identity: range1971583
display id: range1971583
version: 1
direction: direction/forward
top level: BBa_K145150
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1971585
display id: annotation1971585
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1
type: Range
persistent identity: range1971585
display id: range1971585
version: 1
direction: direction/forward
top level: BBa_K145150
start: 42
end: 47
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1971586
display id: annotation1971586
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1
type: Range
persistent identity: range1971586
display id: range1971586
version: 1
direction: direction/forward
top level: BBa_K145150
start: 46
end: 63
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1971584
display id: annotation1971584
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1
type: Range
persistent identity: range1971584
display id: range1971584
version: 1
direction: direction/forward
top level: BBa_K145150
start: 23
end: 40
orientation: inline

Lux-box
type: SequenceAnnotation
persistent identity: annotation1971582
display id: annotation1971582
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1
type: Range
persistent identity: range1971582
display id: range1971582
version: 1
direction: direction/forward
top level: BBa_K145150
start: 1
end: 20
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732434
display id: BBa_I732434
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732434_sequence/1

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (p22 cII regulated)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR3
sequence annotation: BBa_R0053
sequence annotation: OR2
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
direction: direction/forward
top level: cII p22
start: 8
end: 13
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 3
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 54
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
direction: direction/forward
top level: cII p22
start: 11
end: 28
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
direction: direction/forward
top level: cII p22
start: 18
end: 23
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
direction: direction/forward
top level: cII p22
start: 30
end: 35
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
direction: direction/forward
top level: cII p22
start: 34
end: 51
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732442
display id: BBa_I732442
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732442_sequence/1

Pnot(O24)
type: ComponentDefinition
persistent identity: BBa I732274
display id: BBa_I732274
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O24)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732274_sequence/1

BBa_K1165003
type: ComponentDefinition
persistent identity: BBa K1165003
display id: BBa_K1165003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 2 of XOR Gate 1
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Synthetic Extended Arms
sequence annotation: Target Binding Arms
sequence annotation: Target-Binding Arms
sequence annotation: TAA Bump
sequence annotation: Catalytic Core
sequence: https://synbiohub.org/public/igem/BBa_K1165003_sequence/1

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365209
display id: annotation2365209
version: 1
top level: BBa_K1165003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365209/range2365209/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365209/range2365209/1
type: Range
persistent identity: range2365209
display id: range2365209
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 1
end: 30
orientation: inline

Target Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365214
display id: annotation2365214
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365214/range2365214/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365214/range2365214/1
type: Range
persistent identity: range2365214
display id: range2365214
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 70
end: 81
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365215
display id: annotation2365215
version: 1
top level: BBa_K1165003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365215/range2365215/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365215/range2365215/1
type: Range
persistent identity: range2365215
display id: range2365215
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 82
end: 111
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365210
display id: annotation2365210
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365210/range2365210/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365210/range2365210/1
type: Range
persistent identity: range2365210
display id: range2365210
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 32
end: 35
orientation: inline

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2365211
display id: annotation2365211
version: 1
top level: BBa_K1165003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365211/range2365211/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365211/range2365211/1
type: Range
persistent identity: range2365211
display id: range2365211
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 36
end: 38
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365212
display id: annotation2365212
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365212/range2365212/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365212/range2365212/1
type: Range
persistent identity: range2365212
display id: range2365212
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 39
end: 43
orientation: inline

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2365213
display id: annotation2365213
version: 1
top level: BBa_K1165003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365213/range2365213/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365213/range2365213/1
type: Range
persistent identity: range2365213
display id: range2365213
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 44
end: 69
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR2
sequence annotation: BBa_R0051
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

BBa_K086033
type: ComponentDefinition
persistent identity: BBa K086033
display id: BBa_K086033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: cleaned
mutable provenance: Lac O and sigma 38 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_4
sequence: https://synbiohub.org/public/igem/BBa_K086033_sequence/1

P38_4
type: SequenceAnnotation
persistent identity: annotation1995775
display id: annotation1995775
version: 1
top level: BBa_K086033
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086033/annotation1995775/range1995775/1

https://synbiohub.org/public/igem/BBa_K086033/annotation1995775/range1995775/1
type: Range
persistent identity: range1995775
display id: range1995775
version: 1
direction: direction/forward
top level: BBa_K086033
start: 1
end: 55
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732413
display id: BBa_I732413
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732413_sequence/1

Pnot(O21)
type: ComponentDefinition
persistent identity: BBa I732273
display id: BBa_I732273
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O21)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732273_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732418
display id: BBa_I732418
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732418_sequence/1

cI lam
type: ComponentDefinition
persistent identity: BBa R1051
display id: BBa_R1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Modified to comply with BBa_S0001: TTGACA-17N-GATACT Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR2
sequence annotation: BBa_R1051
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_R1051_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2075
display id: annotation2075
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1
type: Range
persistent identity: range2075
display id: range2075
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2077
display id: annotation2077
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1
type: Range
persistent identity: range2077
display id: range2077
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation7074
display id: annotation7074
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1

https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1
type: Range
persistent identity: range7074
display id: range7074
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2078
display id: annotation2078
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1
type: Range
persistent identity: range2078
display id: range2078
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2076
display id: annotation2076
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1
type: Range
persistent identity: range2076
display id: range2076
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

BBa_I739101
type: ComponentDefinition
persistent identity: BBa I739101
display id: BBa_I739101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (constitutive / TetR, negative)
creator: Stefan Luzi
created: 2007-10-11T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sdfasdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23100
sequence annotation: TetR 1
sequence annotation: Scar
sequence annotation: R0040 (2x TetR 1)
sequence: https://synbiohub.org/public/igem/BBa_I739101_sequence/1

J23100
type: SequenceAnnotation
persistent identity: annotation1946100
display id: annotation1946100
version: 1
top level: BBa_I739101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946100/range1946100/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946100/range1946100/1
type: Range
persistent identity: range1946100
display id: range1946100
version: 1
direction: direction/forward
top level: BBa_I739101
start: 1
end: 35
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946101
display id: annotation1946101
version: 1
top level: BBa_I739101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946101/range1946101/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946101/range1946101/1
type: Range
persistent identity: range1946101
display id: range1946101
version: 1
direction: direction/forward
top level: BBa_I739101
start: 64
end: 83
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946102
display id: annotation1946102
version: 1
top level: BBa_I739101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946102/range1946102/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946102/range1946102/1
type: Range
persistent identity: range1946102
display id: range1946102
version: 1
direction: direction/forward
top level: BBa_I739101
start: 44
end: 63
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1946099
display id: annotation1946099
version: 1
top level: BBa_I739101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946099/range1946099/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946099/range1946099/1
type: Range
persistent identity: range1946099
display id: range1946099
version: 1
direction: direction/forward
top level: BBa_I739101
start: 36
end: 43
orientation: inline

R0040 (2x TetR 1)
type: SequenceAnnotation
persistent identity: annotation1946257
display id: annotation1946257
version: 1
top level: BBa_I739101
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946257/range1946257/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946257/range1946257/1
type: Range
persistent identity: range1946257
display id: range1946257
version: 1
direction: direction/forward
top level: BBa_I739101
start: 44
end: 83
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732433
display id: BBa_I732433
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732433_sequence/1

BBa_I739102
type: ComponentDefinition
persistent identity: BBa I739102
display id: BBa_I739102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (cI, negative / TetR, negative)
creator: Stefan Luzi
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR 1
sequence annotation: R0040 (2x TetR 1)
sequence annotation: Scar
sequence annotation: R0051
sequence: https://synbiohub.org/public/igem/BBa_I739102_sequence/1

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946932
display id: annotation1946932
version: 1
top level: BBa_I739102
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946932/range1946932/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946932/range1946932/1
type: Range
persistent identity: range1946932
display id: range1946932
version: 1
direction: direction/forward
top level: BBa_I739102
start: 78
end: 97
orientation: inline

R0040 (2x TetR 1)
type: SequenceAnnotation
persistent identity: annotation1946933
display id: annotation1946933
version: 1
top level: BBa_I739102
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946933/range1946933/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946933/range1946933/1
type: Range
persistent identity: range1946933
display id: range1946933
version: 1
direction: direction/forward
top level: BBa_I739102
start: 58
end: 97
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1946931
display id: annotation1946931
version: 1
top level: BBa_I739102
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946931/range1946931/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946931/range1946931/1
type: Range
persistent identity: range1946931
display id: range1946931
version: 1
direction: direction/forward
top level: BBa_I739102
start: 50
end: 57
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946934
display id: annotation1946934
version: 1
top level: BBa_I739102
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946934/range1946934/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946934/range1946934/1
type: Range
persistent identity: range1946934
display id: range1946934
version: 1
direction: direction/forward
top level: BBa_I739102
start: 58
end: 77
orientation: inline

R0051
type: SequenceAnnotation
persistent identity: annotation1946930
display id: annotation1946930
version: 1
top level: BBa_I739102
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946930/range1946930/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946930/range1946930/1
type: Range
persistent identity: range1946930
display id: range1946930
version: 1
direction: direction/forward
top level: BBa_I739102
start: 1
end: 49
orientation: inline

BBa_K1031742
type: ComponentDefinition
persistent identity: BBa K1031742
display id: BBa_K1031742
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-PaaX
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 109-PaaX-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PaaX
sequence annotation: BBa_B0034
sequence annotation: BBa_J23109
sequence: https://synbiohub.org/public/igem/BBa_K1031742_sequence/1

PaaX
type: SequenceAnnotation
persistent identity: annotation2357489
display id: annotation2357489
version: 1
top level: BBa_K1031742
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031742/annotation2357489/range2357489/1

https://synbiohub.org/public/igem/BBa_K1031742/annotation2357489/range2357489/1
type: Range
persistent identity: range2357489
display id: range2357489
version: 1
direction: direction/forward
top level: BBa_K1031742
start: 62
end: 1012
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357490
display id: annotation2357490
version: 1
top level: BBa_K1031742
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031742/annotation2357490/range2357490/1

https://synbiohub.org/public/igem/BBa_K1031742/annotation2357490/range2357490/1
type: Range
persistent identity: range2357490
display id: range2357490
version: 1
direction: direction/forward
top level: BBa_K1031742
start: 44
end: 55
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2357488
display id: annotation2357488
version: 1
top level: BBa_K1031742
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031742/annotation2357488/range2357488/1

https://synbiohub.org/public/igem/BBa_K1031742/annotation2357488/range2357488/1
type: Range
persistent identity: range2357488
display id: range2357488
version: 1
direction: direction/forward
top level: BBa_K1031742
start: 1
end: 35
orientation: inline

BBa_K091106
type: ComponentDefinition
persistent identity: BBa K091106
display id: BBa_K091106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrA/cI hybrid promoter
creator: Andrew Gordon
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This hybrid promoter uses the LsrA promoter that is capable of being repressed by LsrR - induction can occur with phospho-AI-2. Binding sites for cI repressor also occur. As a result the promoter is off in the absence of AI-2 and on in the presence of AI-2, but always off in the presence of cI.
mutable notes: The promoter region for LsrA should remain unaltered while cI binding sites (OR1 and OR2) are introduced downstream. The OR2 sites with cI bound should not allow transcription. The cI binding sites have a natural 6 bp spacer between them. It would be desirable to position the cI binding sites immediately downstream of the -10 region of the LsrA promoter. However, experimental evidence for the transciption start (+1) appears to be lacking.
mutable provenance: The Missouri Western/Davidson team designed primers for LsrA and extracted the DNA from E. coli MG1655. The CI portion was pulled from the registry as part number R0051.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K091106_sequence/1

BBa_K086032
type: ComponentDefinition
persistent identity: BBa K086032
display id: BBa_K086032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: cleaned
mutable provenance: Lac O and Sigma 38 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_3
sequence: https://synbiohub.org/public/igem/BBa_K086032_sequence/1

P38_3
type: SequenceAnnotation
persistent identity: annotation1995774
display id: annotation1995774
version: 1
top level: BBa_K086032
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086032/annotation1995774/range1995774/1

https://synbiohub.org/public/igem/BBa_K086032/annotation1995774/range1995774/1
type: Range
persistent identity: range1995774
display id: range1995774
version: 1
direction: direction/forward
top level: BBa_K086032
start: 1
end: 55
orientation: inline

BBa_J05210
type: ComponentDefinition
persistent identity: BBa J05210
display id: BBa_J05210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modifed Prm+ Promoter
creator: Alexander Roth
created: 2005-09-05T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is identical to BBa_I12007 exept that some base has been modifed to allow for additional ZFP repressors to bind.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: ZFP binding sites
sequence annotation: OR2
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_J05210_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1689961
display id: annotation1689961
version: 1
top level: BBa_J05210
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689961/range1689961/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689961/range1689961/1
type: Range
persistent identity: range1689961
display id: range1689961
version: 1
direction: direction/forward
top level: BBa_J05210
start: 71
end: 76
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1689959
display id: annotation1689959
version: 1
top level: BBa_J05210
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689959/range1689959/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689959/range1689959/1
type: Range
persistent identity: range1689959
display id: range1689959
version: 1
direction: direction/forward
top level: BBa_J05210
start: 48
end: 53
orientation: inline

ZFP binding sites
type: SequenceAnnotation
persistent identity: annotation1689960
display id: annotation1689960
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689960/range1689960/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689960/range1689960/1
type: Range
persistent identity: range1689960
display id: range1689960
version: 1
direction: direction/forward
top level: BBa_J05210
start: 55
end: 66
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1689958
display id: annotation1689958
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689958/range1689958/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689958/range1689958/1
type: Range
persistent identity: range1689958
display id: range1689958
version: 1
direction: direction/forward
top level: BBa_J05210
start: 33
end: 49
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1689957
display id: annotation1689957
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689957/range1689957/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689957/range1689957/1
type: Range
persistent identity: range1689957
display id: range1689957
version: 1
direction: direction/forward
top level: BBa_J05210
start: 9
end: 25
orientation: inline

Pnot(O66)
type: ComponentDefinition
persistent identity: BBa I732206
display id: BBa_I732206
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O66)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732206_sequence/1

BBa_K137050
type: ComponentDefinition
persistent identity: BBa K137050
display id: BBa_K137050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 650 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 650 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137050_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968023
display id: annotation1968023
version: 1
top level: BBa_K137050
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137050/annotation1968023/range1968023/1

https://synbiohub.org/public/igem/BBa_K137050/annotation1968023/range1968023/1
type: Range
persistent identity: range1968023
display id: range1968023
version: 1
direction: direction/reverse
top level: BBa_K137050
start: 1
end: 54
orientation: inline

BBa_I715003
type: ComponentDefinition
persistent identity: BBa I715003
display id: BBa_I715003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hybrid pLac with UV5 mutation
creator: Michael Waters
created: 2007-07-02T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _120_
m user id: 0
owner id: 1761
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter sequence is a derivative of part BBa_R0011 which is a -35 and -10 recognition site with two lac operator 1's inserted in the spacer region of the RNAP recognition region and before the -35 RANP recognition region. This part utilizes the UV5 double mutation at the -10 RNAP recognition site to recruit RNAP without the help of a CAP binding region.
mutable notes: The length of the fragment makes it cumbersome to gel purify
mutable provenance: E.Coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 RNAP binding
sequence annotation: -10 RNAP binding
sequence annotation: lac 01
sequence annotation: UV5 -10 RNAP recognition region double mutation
sequence: https://synbiohub.org/public/igem/BBa_I715003_sequence/1

-35 RNAP binding
type: SequenceAnnotation
persistent identity: annotation1936228
display id: annotation1936228
version: 1
top level: BBa_I715003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936228/range1936228/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936228/range1936228/1
type: Range
persistent identity: range1936228
display id: range1936228
version: 1
direction: direction/forward
top level: BBa_I715003
start: 20
end: 25
orientation: inline

-10 RNAP binding
type: SequenceAnnotation
persistent identity: annotation1936231
display id: annotation1936231
version: 1
top level: BBa_I715003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936231/range1936231/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936231/range1936231/1
type: Range
persistent identity: range1936231
display id: range1936231
version: 1
direction: direction/forward
top level: BBa_I715003
start: 43
end: 47
orientation: inline

lac 01
type: SequenceAnnotation
persistent identity: annotation1936229
display id: annotation1936229
version: 1
top level: BBa_I715003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936229/range1936229/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936229/range1936229/1
type: Range
persistent identity: range1936229
display id: range1936229
version: 1
direction: direction/forward
top level: BBa_I715003
start: 3
end: 19
orientation: inline

lac 01
type: SequenceAnnotation
persistent identity: annotation1936230
display id: annotation1936230
version: 1
top level: BBa_I715003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936230/range1936230/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936230/range1936230/1
type: Range
persistent identity: range1936230
display id: range1936230
version: 1
direction: direction/forward
top level: BBa_I715003
start: 26
end: 42
orientation: inline

UV5 -10 RNAP recognition region double mutation
type: SequenceAnnotation
persistent identity: annotation1936232
display id: annotation1936232
version: 1
top level: BBa_I715003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936232/range1936232/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936232/range1936232/1
type: Range
persistent identity: range1936232
display id: range1936232
version: 1
direction: direction/forward
top level: BBa_I715003
start: 43
end: 47
orientation: inline

BBa_I756021
type: ComponentDefinition
persistent identity: BBa I756021
display id: BBa_I756021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV promoter with Lambda Operator
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CMV promoter with Lambda Operator
mutable notes: CMV promoter with Lambda Operator
mutable provenance: CMV promoter with Lambda Operator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756021_sequence/1

BBa_K332032
type: ComponentDefinition
persistent identity: BBa K332032
display id: BBa_K332032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter+37℃ induced RBS+tetR+double terminator+Ptet+RBS+GFP+double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein. Therefore, we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J61048
component: BBa_K332031
component: BBa_R0040
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_R0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_J61048
sequence annotation: BBa_K332031
sequence: https://synbiohub.org/public/igem/BBa_K332032_sequence/1

BBa_J61048
type: Component
persistent identity: component2273256
display id: component2273256
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_K332031
type: Component
persistent identity: component2273246
display id: component2273246
version: 1
top level: BBa_K332032
access: public
definition: BBa_K332031

BBa_R0040
type: Component
persistent identity: component2273247
display id: component2273247
version: 1
top level: BBa_K332032
access: public
definition: p(tetR)

BBa_B0034
type: Component
persistent identity: component2273253
display id: component2273253
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2273255
display id: component2273255
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2273247
display id: annotation2273247
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
type: Range
persistent identity: range2273247
display id: range2273247
version: 1
direction: direction/forward
top level: BBa_K332032
start: 916
end: 969
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2273255
display id: annotation2273255
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
type: Range
persistent identity: range2273255
display id: range2273255
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 996
end: 1715
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273253
display id: annotation2273253
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
type: Range
persistent identity: range2273253
display id: range2273253
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 978
end: 989
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2273256
display id: annotation2273256
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
type: Range
persistent identity: range2273256
display id: range2273256
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1724
end: 1836
orientation: inline

BBa_K332031
type: SequenceAnnotation
persistent identity: annotation2273246
display id: annotation2273246
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
component: BBa_K332031

https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
type: Range
persistent identity: range2273246
display id: range2273246
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1
end: 907
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732414
display id: BBa_I732414
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732414_sequence/1

BBa_R2111
type: ComponentDefinition
persistent identity: BBa R2111
display id: BBa_R2111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was rationally designed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 site
sequence annotation: -10 site
sequence annotation: C2003 operator site
sequence annotation: transcription start site
sequence: https://synbiohub.org/public/igem/BBa_R2111_sequence/1

-35 site
type: SequenceAnnotation
persistent identity: annotation1903920
display id: annotation1903920
version: 1
top level: BBa_R2111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903920/range1903920/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903920/range1903920/1
type: Range
persistent identity: range1903920
display id: range1903920
version: 1
direction: direction/forward
top level: BBa_R2111
start: 17
end: 22
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903921
display id: annotation1903921
version: 1
top level: BBa_R2111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903921/range1903921/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903921/range1903921/1
type: Range
persistent identity: range1903921
display id: range1903921
version: 1
direction: direction/forward
top level: BBa_R2111
start: 40
end: 45
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1903923
display id: annotation1903923
version: 1
top level: BBa_R2111
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903923/range1903923/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903923/range1903923/1
type: Range
persistent identity: range1903923
display id: range1903923
version: 1
direction: direction/forward
top level: BBa_R2111
start: 23
end: 34
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903922
display id: annotation1903922
version: 1
top level: BBa_R2111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903922/range1903922/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903922/range1903922/1
type: Range
persistent identity: range1903922
display id: range1903922
version: 1
direction: direction/forward
top level: BBa_R2111
start: 53
end: 53
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732404
display id: BBa_I732404
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732401.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: START
sequence annotation: stop
sequence annotation: polya
sequence annotation: BBa_B0012
sequence annotation: conserved
sequence annotation: STOP
sequence annotation: BBa_E0044
sequence annotation: BBa_I732006
sequence annotation: BBa_B0034
sequence annotation: BglII
sequence annotation: BamHI
sequence annotation: stem_loop
sequence annotation: lacZ
sequence annotation: T7 TE
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I732404_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1947690
display id: annotation1947690
version: 1
top level: U061O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947690/range1947690/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947690/range1947690/1
type: Range
persistent identity: range1947690
display id: range1947690
version: 1
direction: direction/forward
top level: U061O11+D0
start: 46
end: 48
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1947700
display id: annotation1947700
version: 1
top level: U061O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947700/range1947700/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947700/range1947700/1
type: Range
persistent identity: range1947700
display id: range1947700
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1194
end: 1194
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1947699
display id: annotation1947699
version: 1
top level: U061O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947699/range1947699/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947699/range1947699/1
type: Range
persistent identity: range1947699
display id: range1947699
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1188
end: 1201
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1947697
display id: annotation1947697
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947697/range1947697/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947697/range1947697/1
type: Range
persistent identity: range1947697
display id: range1947697
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1161
end: 1201
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1947693
display id: annotation1947693
version: 1
top level: U061O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947693/range1947693/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947693/range1947693/1
type: Range
persistent identity: range1947693
display id: range1947693
version: 1
direction: direction/forward
top level: U061O11+D0
start: 292
end: 295
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1947691
display id: annotation1947691
version: 1
top level: U061O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947691/range1947691/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947691/range1947691/1
type: Range
persistent identity: range1947691
display id: range1947691
version: 1
direction: direction/forward
top level: U061O11+D0
start: 274
end: 279
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1947694
display id: annotation1947694
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947694/range1947694/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947694/range1947694/1
type: Range
persistent identity: range1947694
display id: range1947694
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 306
end: 1064
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1947688
display id: annotation1947688
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947688/range1947688/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947688/range1947688/1
type: Range
persistent identity: range1947688
display id: range1947688
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947686
display id: annotation1947686
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947686/range1947686/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947686/range1947686/1
type: Range
persistent identity: range1947686
display id: range1947686
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 28
end: 39
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1947684
display id: annotation1947684
version: 1
top level: U061O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947684/range1947684/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947684/range1947684/1
type: Range
persistent identity: range1947684
display id: range1947684
version: 1
direction: direction/forward
top level: U061O11+D0
start: 3
end: 8
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1947687
display id: annotation1947687
version: 1
top level: U061O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947687/range1947687/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947687/range1947687/1
type: Range
persistent identity: range1947687
display id: range1947687
version: 1
direction: direction/forward
top level: U061O11+D0
start: 32
end: 35
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1947685
display id: annotation1947685
version: 1
top level: U061O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947685/range1947685/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947685/range1947685/1
type: Range
persistent identity: range1947685
display id: range1947685
version: 1
direction: direction/forward
top level: U061O11+D0
start: 12
end: 17
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947692
display id: annotation1947692
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947692/range1947692/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947692/range1947692/1
type: Range
persistent identity: range1947692
display id: range1947692
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 288
end: 299
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1947696
display id: annotation1947696
version: 1
top level: U061O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947696/range1947696/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947696/range1947696/1
type: Range
persistent identity: range1947696
display id: range1947696
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1084
end: 1127
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1947689
display id: annotation1947689
version: 1
top level: U061O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947689/range1947689/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947689/range1947689/1
type: Range
persistent identity: range1947689
display id: range1947689
version: 1
direction: direction/forward
top level: U061O11+D0
start: 46
end: 279
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1947698
display id: annotation1947698
version: 1
top level: U061O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947698/range1947698/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947698/range1947698/1
type: Range
persistent identity: range1947698
display id: range1947698
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1168
end: 1187
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1947695
display id: annotation1947695
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947695/range1947695/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947695/range1947695/1
type: Range
persistent identity: range1947695
display id: range1947695
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1073
end: 1152
orientation: inline

MioC
type: ComponentDefinition
persistent identity: BBa K101017
display id: BBa_K101017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MioC Promoter (DNAa-Repressed Promoter)
creator: Karl Petersen
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _205_
m user id: 0
owner id: 2728
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The MioC promoter is a cell-cycle dependent promoter that is repressed before initiation of replication and depressed shortly after. The regulation of the MioC gene is overseen by several DNAa proteins, which bind along the promoter. This particular MioC promoter was isolated from K-12 (JM109). This promoter can be used in series with a reporter to signal only during a given part of the cell cycle.
mutable notes: Site directed mutagenesis was used to create a Spe1 restriction site.
mutable provenance: The source of this part is from the genomic dna of E.coli (JM109).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: DNAa Binding Box
sequence annotation: MioC Promoter
sequence: https://synbiohub.org/public/igem/BBa_K101017_sequence/1

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992605
display id: annotation1992605
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992605/range1992605/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992605/range1992605/1
type: Range
persistent identity: range1992605
display id: range1992605
version: 1
direction: direction/forward
top level: MioC
start: 283
end: 292
orientation: inline

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992607
display id: annotation1992607
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992607/range1992607/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992607/range1992607/1
type: Range
persistent identity: range1992607
display id: range1992607
version: 1
direction: direction/forward
top level: MioC
start: 273
end: 279
orientation: inline

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992609
display id: annotation1992609
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992609/range1992609/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992609/range1992609/1
type: Range
persistent identity: range1992609
display id: range1992609
version: 1
direction: direction/forward
top level: MioC
start: 159
end: 167
orientation: inline

MioC Promoter
type: SequenceAnnotation
persistent identity: annotation1992615
display id: annotation1992615
version: 1
top level: MioC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992615/range1992615/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992615/range1992615/1
type: Range
persistent identity: range1992615
display id: range1992615
version: 1
direction: direction/forward
top level: MioC
start: 131
end: 319
orientation: inline

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992601
display id: annotation1992601
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992601/range1992601/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992601/range1992601/1
type: Range
persistent identity: range1992601
display id: range1992601
version: 1
direction: direction/forward
top level: MioC
start: 295
end: 303
orientation: inline

TrpR
type: ComponentDefinition
persistent identity: BBa K191007
display id: BBa_K191007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Trp promoter
creator: Le Thanh Tu NGUYEN
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _287_
m user id: 0
owner id: 4198
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter of Tryptophan operon ( Tryptophan operon is an operon that codes for the components of tryptophan's synthesis)
mutable notes: 2 SpeI enzyme restriction sites on Trp operon sequence.
mutable provenance: Sequence of Trp operon was found at : http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00067 Part was added to the system as the upstream sequence of the forward primer in a PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K191007_sequence/1

BBa_K1886017
type: ComponentDefinition
persistent identity: BBa K1886017
display id: BBa_K1886017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: broken_Ptet
creator: Lejian Jiang
created: 2016-10-11T11:00:00Z
modified: 2016-10-17T11:34:11Z
discontinued: false
dominant: false
group u list: _2351_
m user id: 29387
owner id: 29387
owning group id: 9
status: status/Planning
bookmark: false
mutable description: xxx
mutable notes: xxx
mutable provenance: xxx
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1886017_sequence/1

BBa_K1824895
type: ComponentDefinition
persistent identity: BBa K1824895
display id: BBa_K1824895
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet + RBS
creator: Zixu Wang
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T09:46:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A inducible promoter plus a strong RBS B0034, repressed by TetR, induced by tetracycline and aTc.
mutable notes: This part should be used along with TetR.
mutable provenance: B0034 is based on Elowitz repressilator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_K1824890
component: BBa_K1824889
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K1824889
sequence annotation: BBa_K1824890
sequence: https://synbiohub.org/public/igem/BBa_K1824895_sequence/1

BBa_R0040
type: Component
persistent identity: component2452330
display id: component2452330
version: 1
top level: BBa_K1824895
access: public
definition: p(tetR)

BBa_K1824890
type: Component
persistent identity: component2452338
display id: component2452338
version: 1
top level: BBa_K1824895
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824890/1

BBa_K1824889
type: Component
persistent identity: component2452335
display id: component2452335
version: 1
top level: BBa_K1824895
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_B0034
type: Component
persistent identity: component2452337
display id: component2452337
version: 1
top level: BBa_K1824895
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2452330
display id: annotation2452330
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452330/range2452330/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452330/range2452330/1
type: Range
persistent identity: range2452330
display id: range2452330
version: 1
direction: direction/forward
top level: BBa_K1824895
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2452337
display id: annotation2452337
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452337/range2452337/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452337/range2452337/1
type: Range
persistent identity: range2452337
display id: range2452337
version: 1
direction: direction/reverse
top level: BBa_K1824895
start: 63
end: 74
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2452335
display id: annotation2452335
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452335/range2452335/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452335/range2452335/1
type: Range
persistent identity: range2452335
display id: range2452335
version: 1
direction: direction/reverse
top level: BBa_K1824895
start: 55
end: 62
orientation: inline

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2452338
display id: annotation2452338
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452338/range2452338/1
component: BBa_K1824890

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452338/range2452338/1
type: Range
persistent identity: range2452338
display id: range2452338
version: 1
direction: direction/reverse
top level: BBa_K1824895
start: 75
end: 80
orientation: inline

BBa_K921001
type: ComponentDefinition
persistent identity: BBa K921001
display id: BBa_K921001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant II)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: Initiation
sequence annotation: LacO
sequence annotation: T7 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K921001_sequence/1

Initiation
type: SequenceAnnotation
persistent identity: annotation2205298
display id: annotation2205298
version: 1
top level: BBa_K921001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1
type: Range
persistent identity: range2205298
display id: range2205298
version: 1
direction: direction/forward
top level: BBa_K921001
start: 18
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205297
display id: annotation2205297
version: 1
top level: BBa_K921001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1
type: Range
persistent identity: range2205297
display id: range2205297
version: 1
direction: direction/forward
top level: BBa_K921001
start: 22
end: 48
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205296
display id: annotation2205296
version: 1
top level: BBa_K921001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1
type: Range
persistent identity: range2205296
display id: range2205296
version: 1
direction: direction/forward
top level: BBa_K921001
start: 1
end: 20
orientation: inline

BBa_I732450
type: ComponentDefinition
persistent identity: BBa I732450
display id: BBa_I732450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O26+D062NUL)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732450_sequence/1

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732432
display id: BBa_I732432
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732432_sequence/1

BBa_K921002
type: ComponentDefinition
persistent identity: BBa K921002
display id: BBa_K921002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant III)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: Initiation
sequence annotation: T7 Promoter
sequence annotation: LacO
sequence: https://synbiohub.org/public/igem/BBa_K921002_sequence/1

Initiation
type: SequenceAnnotation
persistent identity: annotation2205318
display id: annotation2205318
version: 1
top level: BBa_K921002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1
type: Range
persistent identity: range2205318
display id: range2205318
version: 1
direction: direction/forward
top level: BBa_K921002
start: 18
end: 20
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205316
display id: annotation2205316
version: 1
top level: BBa_K921002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1
type: Range
persistent identity: range2205316
display id: range2205316
version: 1
direction: direction/forward
top level: BBa_K921002
start: 1
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205317
display id: annotation2205317
version: 1
top level: BBa_K921002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1
type: Range
persistent identity: range2205317
display id: range2205317
version: 1
direction: direction/forward
top level: BBa_K921002
start: 19
end: 47
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732435
display id: BBa_I732435
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732435_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732401
display id: BBa_I732401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D062NUL)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Our first NAND logical gate
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732401_sequence/1

BBa_K1682012
type: ComponentDefinition
persistent identity: BBa K1682012
display id: BBa_K1682012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PphoA promoter
creator: iGEM15_HKUST-Rice
created: 2015-09-01T11:00:00Z
modified: 2015-09-18T10:16:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2100_
m user id: 21235
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PphoA promoter is a phosphate-responsive promoter. In high concentration of phosphate, it will be repressed while in low phosphate concentration, it will be activated.
mutable notes: N/A
mutable provenance: Native in E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: phospho-phoB
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1682012_sequence/1

phospho-phoB
type: SequenceAnnotation
persistent identity: annotation2476002
display id: annotation2476002
version: 1
top level: BBa_K1682012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476002/range2476002/1

https://synbiohub.org/public/igem/BBa_K1682012/annotation2476002/range2476002/1
type: Range
persistent identity: range2476002
display id: range2476002
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 21
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2476003
display id: annotation2476003
version: 1
top level: BBa_K1682012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476003/range2476003/1

https://synbiohub.org/public/igem/BBa_K1682012/annotation2476003/range2476003/1
type: Range
persistent identity: range2476003
display id: range2476003
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 28
end: 33
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2476004
display id: annotation2476004
version: 1
top level: BBa_K1682012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476004/range2476004/1

https://synbiohub.org/public/igem/BBa_K1682012/annotation2476004/range2476004/1
type: Range
persistent identity: range2476004
display id: range2476004
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 50
end: 56
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa R1052
display id: BBa_R1052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (434 cI regulated)
creator: Maia Mahoney, Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: [Note: This is the same part as R0052 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
mutable notes: Wild-type promoter modified to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR2
sequence annotation: start
sequence annotation: OR1
sequence annotation: -10
sequence annotation: BBa_R1052
sequence: https://synbiohub.org/public/igem/BBa_R1052_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2082
display id: annotation2082
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2082/range2082/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2082/range2082/1
type: Range
persistent identity: range2082
display id: range2082
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 6
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2080
display id: annotation2080
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2080/range2080/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2080/range2080/1
type: Range
persistent identity: range2080
display id: range2080
version: 1
direction: direction/forward
top level: cI 434
start: 8
end: 21
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2084
display id: annotation2084
version: 1
top level: cI 434
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2084/range2084/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2084/range2084/1
type: Range
persistent identity: range2084
display id: range2084
version: 1
direction: direction/forward
top level: cI 434
start: 35
end: 37
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2081
display id: annotation2081
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2081/range2081/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2081/range2081/1
type: Range
persistent identity: range2081
display id: range2081
version: 1
direction: direction/forward
top level: cI 434
start: 30
end: 43
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2087
display id: annotation2087
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2087/range2087/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2087/range2087/1
type: Range
persistent identity: range2087
display id: range2087
version: 1
direction: direction/forward
top level: cI 434
start: 24
end: 29
orientation: inline

BBa_R1052
type: SequenceAnnotation
persistent identity: annotation7075
display id: annotation7075
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1052/annotation7075/range7075/1

https://synbiohub.org/public/igem/BBa_R1052/annotation7075/range7075/1
type: Range
persistent identity: range7075
display id: range7075
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 46
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2083
display id: annotation2083
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2083/range2083/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2083/range2083/1
type: Range
persistent identity: range2083
display id: range2083
version: 1
direction: direction/forward
top level: cI 434
start: 2
end: 7
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2079
display id: annotation2079
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2079/range2079/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2079/range2079/1
type: Range
persistent identity: range2079
display id: range2079
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 43
orientation: inline

Pnot(O55)
type: ComponentDefinition
persistent identity: BBa I732205
display id: BBa_I732205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O55)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732205_sequence/1

BBa_I739104
type: ComponentDefinition
persistent identity: BBa I739104
display id: BBa_I739104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (LuxR/HSL, positive / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: Scar
sequence annotation: R0062
sequence annotation: R0053 (2x OR1)
sequence: https://synbiohub.org/public/igem/BBa_I739104_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1947894
display id: annotation1947894
version: 1
top level: BBa_I739104
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947894/range1947894/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947894/range1947894/1
type: Range
persistent identity: range1947894
display id: range1947894
version: 1
direction: direction/forward
top level: BBa_I739104
start: 83
end: 101
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1947892
display id: annotation1947892
version: 1
top level: BBa_I739104
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947892/range1947892/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947892/range1947892/1
type: Range
persistent identity: range1947892
display id: range1947892
version: 1
direction: direction/forward
top level: BBa_I739104
start: 56
end: 63
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947893
display id: annotation1947893
version: 1
top level: BBa_I739104
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947893/range1947893/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947893/range1947893/1
type: Range
persistent identity: range1947893
display id: range1947893
version: 1
direction: direction/forward
top level: BBa_I739104
start: 64
end: 82
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation1947891
display id: annotation1947891
version: 1
top level: BBa_I739104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947891/range1947891/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947891/range1947891/1
type: Range
persistent identity: range1947891
display id: range1947891
version: 1
direction: direction/forward
top level: BBa_I739104
start: 1
end: 55
orientation: inline

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947895
display id: annotation1947895
version: 1
top level: BBa_I739104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947895/range1947895/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947895/range1947895/1
type: Range
persistent identity: range1947895
display id: range1947895
version: 1
direction: direction/forward
top level: BBa_I739104
start: 64
end: 101
orientation: inline

BBa_J31013
type: ComponentDefinition
persistent identity: BBa J31013
display id: BBa_J31013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac Backwards [cf. BBa_R0010]
creator: Karmella Haynes
created: 2007-04-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter from the lack operon cloned in the reverse orientation.
mutable notes: Promoter from the lack operon cloned in the reverse orientation.
mutable provenance: BBa_R0010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: end of LacI coding region (inactive)
sequence annotation: -35
sequence annotation: CAP binding site
sequence annotation: R0010 reverse
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J31013_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1928673
display id: annotation1928673
version: 1
top level: BBa_J31013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928673/range1928673/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928673/range1928673/1
type: Range
persistent identity: range1928673
display id: range1928673
version: 1
direction: direction/forward
top level: BBa_J31013
start: 1
end: 34
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1928669
display id: annotation1928669
version: 1
top level: BBa_J31013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928669/range1928669/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928669/range1928669/1
type: Range
persistent identity: range1928669
display id: range1928669
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 113
end: 200
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1928671
display id: annotation1928671
version: 1
top level: BBa_J31013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928671/range1928671/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928671/range1928671/1
type: Range
persistent identity: range1928671
display id: range1928671
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 58
end: 64
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1928670
display id: annotation1928670
version: 1
top level: BBa_J31013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928670/range1928670/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928670/range1928670/1
type: Range
persistent identity: range1928670
display id: range1928670
version: 1
direction: direction/forward
top level: BBa_J31013
start: 74
end: 112
orientation: inline

R0010 reverse
type: SequenceAnnotation
persistent identity: annotation1928668
display id: annotation1928668
version: 1
top level: BBa_J31013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928668/range1928668/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928668/range1928668/1
type: Range
persistent identity: range1928668
display id: range1928668
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1928672
display id: annotation1928672
version: 1
top level: BBa_J31013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928672/range1928672/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928672/range1928672/1
type: Range
persistent identity: range1928672
display id: range1928672
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 35
end: 40
orientation: inline

BBa_K086030
type: ComponentDefinition
persistent identity: BBa K086030
display id: BBa_K086030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: cleaned
mutable provenance: Lac O and sigma 38 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_1
sequence: https://synbiohub.org/public/igem/BBa_K086030_sequence/1

P38_1
type: SequenceAnnotation
persistent identity: annotation1995764
display id: annotation1995764
version: 1
top level: BBa_K086030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086030/annotation1995764/range1995764/1

https://synbiohub.org/public/igem/BBa_K086030/annotation1995764/range1995764/1
type: Range
persistent identity: range1995764
display id: range1995764
version: 1
direction: direction/forward
top level: BBa_K086030
start: 1
end: 55
orientation: inline

pRecA
type: ComponentDefinition
persistent identity: BBa K093000
display id: BBa_K093000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRecA with LexA binding site
creator: Julian Wiegelmann, Danielle Nash
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a pRecA promoter with LexA binding sites. LexA is cleaved by RecA when RecA is upregulated due to induction of the SOS response triggered by single stranded DNA. For this part to work it must be in a RecA+ strain of E. coli such as TG1
mutable notes: How far past the start site of transcription do you need to go in order for the promoter to drive transcription? The promoter has been confirmed to drive transcription in a regular DH5alpha strain, so it the answer to the question is, 3 bp before the suffix, maybe less. The LexA binding site is in the core region of the promoter (between the -35 and -10 sigma 70 binding sites). According to Robert Sidney Cox III and colleagues in their paper "Programming ene expression with combinatorial promoters, the core region is the most effective region for binding of repressor molecules.
mutable provenance: synthetic. Oligonucleotides were designed to form the double stranded molecule with sticky ends for direct ligation into a biobrick vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: LexA binding
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K093000_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1989749
display id: annotation1989749
version: 1
top level: pRecA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K093000/annotation1989749/range1989749/1

https://synbiohub.org/public/igem/BBa_K093000/annotation1989749/range1989749/1
type: Range
persistent identity: range1989749
display id: range1989749
version: 1
direction: direction/forward
top level: pRecA
start: 11
end: 17
orientation: inline

LexA binding
type: SequenceAnnotation
persistent identity: annotation1989779
display id: annotation1989779
version: 1
top level: pRecA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K093000/annotation1989779/range1989779/1

https://synbiohub.org/public/igem/BBa_K093000/annotation1989779/range1989779/1
type: Range
persistent identity: range1989779
display id: range1989779
version: 1
direction: direction/forward
top level: pRecA
start: 15
end: 35
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1989775
display id: annotation1989775
version: 1
top level: pRecA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K093000/annotation1989775/range1989775/1

https://synbiohub.org/public/igem/BBa_K093000/annotation1989775/range1989775/1
type: Range
persistent identity: range1989775
display id: range1989775
version: 1
direction: direction/forward
top level: pRecA
start: 30
end: 39
orientation: inline

BBa_J64065
type: ComponentDefinition
persistent identity: BBa J64065
display id: BBa_J64065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressed promoter
creator: Jeffrey J Tabor
created: 2007-05-12T11:00:00Z
modified: 2015-05-08T01:08:16Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a LuxR-30C6HSL-independent version of R0065. All DNA upstream of the -10 box is from the constitutive promoter J23119. All DNA downstream from the -10 box down is from R0065. This results in the replacement of the Lux box region for a constitutive promoter region and the maintenance of the two cI operators downstream of the transcription start site.
mutable notes: none
mutable provenance: J23119 + R0065
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: -10
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J64065_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation1932685
display id: annotation1932685
version: 1
top level: BBa_J64065
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932685/range1932685/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932685/range1932685/1
type: Range
persistent identity: range1932685
display id: range1932685
version: 1
direction: direction/forward
top level: BBa_J64065
start: 35
end: 35
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1932682
display id: annotation1932682
version: 1
top level: BBa_J64065
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932682/range1932682/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932682/range1932682/1
type: Range
persistent identity: range1932682
display id: range1932682
version: 1
direction: direction/forward
top level: BBa_J64065
start: 24
end: 29
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1932684
display id: annotation1932684
version: 1
top level: BBa_J64065
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932684/range1932684/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932684/range1932684/1
type: Range
persistent identity: range1932684
display id: range1932684
version: 1
direction: direction/forward
top level: BBa_J64065
start: 58
end: 74
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1932683
display id: annotation1932683
version: 1
top level: BBa_J64065
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932683/range1932683/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932683/range1932683/1
type: Range
persistent identity: range1932683
display id: range1932683
version: 1
direction: direction/forward
top level: BBa_J64065
start: 34
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1932681
display id: annotation1932681
version: 1
top level: BBa_J64065
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932681/range1932681/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932681/range1932681/1
type: Range
persistent identity: range1932681
display id: range1932681
version: 1
direction: direction/forward
top level: BBa_J64065
start: 1
end: 6
orientation: inline

BBa_K112148
type: ComponentDefinition
persistent identity: BBa K112148
display id: BBa_K112148
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phoPp1 magnesium promoter
creator: Madhvi Venkatesh
created: 2008-11-01T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: phoPp1 magnesium promoter
mutable notes: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
mutable provenance: genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112148_sequence/1

BBa_K137046
type: ComponentDefinition
persistent identity: BBa K137046
display id: BBa_K137046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 150 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: tetR promoter with noncoding, spacer DNA upstream of it to make the total length 150 bp
mutable notes: This part is in a series of tetR promoter that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137046_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968019
display id: annotation1968019
version: 1
top level: BBa_K137046
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137046/annotation1968019/range1968019/1

https://synbiohub.org/public/igem/BBa_K137046/annotation1968019/range1968019/1
type: Range
persistent identity: range1968019
display id: range1968019
version: 1
direction: direction/reverse
top level: BBa_K137046
start: 1
end: 54
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732439
display id: BBa_I732439
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732439_sequence/1

BBa_I761011
type: ComponentDefinition
persistent identity: BBa I761011
display id: BBa_I761011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CinR, CinL and glucose controlled promotor
creator: 2007 NYMU_Taiwan iGEM Team
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _140_
m user id: 0
owner id: 2031
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promotor is controlled by cinr, cinl and extrcellular glucose concentration. High extracellular glucose concentration will inhibit this promotor through the action of phosphorylated OmpR. Phosphorylated OmpR will bind the OmpR binding site of the promotor and block the movement of RNA polymerase. In contrast, the combined action of CinR and CinL will turn on the promotor. But only when the extracellular glucose level is low so OmpR dephosphorylate and dissociate from its binding site will this promotor become active. In essence, this promotor function as an AND gate, it will turn on if and only if the extracellular glucose level is low and there are some functional CinR and CinL protein inside the cell.
mutable notes: The key to the function of the promotor is the inhibitory ability of OmpR binding site. To ensure maximum affinity with phosphorylated OmpR, we use all three OmpR binding site.s.
mutable provenance: *BBa_R0078 *BBa_I761001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I761001
component: BBa_R0078
sequence annotation: BBa_I761001
sequence annotation: BBa_R0078
sequence: https://synbiohub.org/public/igem/BBa_I761011_sequence/1

BBa_I761001
type: Component
persistent identity: component1950122
display id: component1950122
version: 1
top level: BBa_I761011
access: public
definition: https://synbiohub.org/public/igem/BBa_I761001/1

BBa_R0078
type: Component
persistent identity: component1950118
display id: component1950118
version: 1
top level: BBa_I761011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0078/1

BBa_I761001
type: SequenceAnnotation
persistent identity: annotation1950122
display id: annotation1950122
version: 1
top level: BBa_I761011
location: https://synbiohub.org/public/igem/BBa_I761011/annotation1950122/range1950122/1
component: BBa_I761001

https://synbiohub.org/public/igem/BBa_I761011/annotation1950122/range1950122/1
type: Range
persistent identity: range1950122
display id: range1950122
version: 1
direction: direction/reverse
top level: BBa_I761011
start: 234
end: 295
orientation: inline

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation1950118
display id: annotation1950118
version: 1
top level: BBa_I761011
location: https://synbiohub.org/public/igem/BBa_I761011/annotation1950118/range1950118/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I761011/annotation1950118/range1950118/1
type: Range
persistent identity: range1950118
display id: range1950118
version: 1
direction: direction/reverse
top level: BBa_I761011
start: 1
end: 225
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732441
display id: BBa_I732441
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732441_sequence/1

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732431
display id: BBa_I732431
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732431_sequence/1

BBa_K266008
type: ComponentDefinition
persistent identity: BBa K266008
display id: BBa_K266008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + Lac inverter
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The BBa_J23100 constitutive promoter with Lac systems inverter
mutable notes: None
mutable provenance: The biobricks BBa_J23100 and BBa_Q04121 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_Q04121
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_Q04121
sequence: https://synbiohub.org/public/igem/BBa_K266008_sequence/1

BBa_Q04121
type: Component
persistent identity: component2262964
display id: component2262964
version: 1
top level: BBa_K266008
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_J23100
type: Component
persistent identity: component2262945
display id: component2262945
version: 1
top level: BBa_K266008
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2262945
display id: annotation2262945
version: 1
top level: BBa_K266008
location: https://synbiohub.org/public/igem/BBa_K266008/annotation2262945/range2262945/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K266008/annotation2262945/range2262945/1
type: Range
persistent identity: range2262945
display id: range2262945
version: 1
direction: direction/reverse
top level: BBa_K266008
start: 1
end: 35
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2262964
display id: annotation2262964
version: 1
top level: BBa_K266008
location: https://synbiohub.org/public/igem/BBa_K266008/annotation2262964/range2262964/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K266008/annotation2262964/range2262964/1
type: Range
persistent identity: range2262964
display id: range2262964
version: 1
direction: direction/reverse
top level: BBa_K266008
start: 44
end: 1414
orientation: inline

NOR1
type: ComponentDefinition
persistent identity: BBa I732351
display id: BBa_I732351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOR Candidate (U037O11D002O22)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O16
mutable notes: N/A
mutable provenance: LacIq
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732351_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732415
display id: BBa_I732415
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732415_sequence/1

BBa_I756020
type: ComponentDefinition
persistent identity: BBa I756020
display id: BBa_I756020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet Operator in SV-40 intron
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tet Operator in SV-40 intron
mutable notes: Tet Operator in SV-40 intron
mutable provenance: Tet Operator in SV-40 intron
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756020_sequence/1

BBa_K266009
type: ComponentDefinition
persistent identity: BBa K266009
display id: BBa_K266009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + Lac inverter + RBS
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The BBa_J23100 constitutive promoter with Lac systems inverter and strong RBS
mutable notes: None
mutable provenance: The biobricks BBa_K266008 and BBa_B0034 where used to build this part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0034
component: BBa_K266008
sequence annotation: BBa_K266008
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K266009_sequence/1

BBa_B0034
type: Component
persistent identity: component2267641
display id: component2267641
version: 1
top level: BBa_K266009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K266008
type: Component
persistent identity: component2267639
display id: component2267639
version: 1
top level: BBa_K266009
access: public
definition: BBa_K266008

BBa_K266008
type: SequenceAnnotation
persistent identity: annotation2267639
display id: annotation2267639
version: 1
top level: BBa_K266009
location: https://synbiohub.org/public/igem/BBa_K266009/annotation2267639/range2267639/1
component: BBa_K266008

https://synbiohub.org/public/igem/BBa_K266009/annotation2267639/range2267639/1
type: Range
persistent identity: range2267639
display id: range2267639
version: 1
direction: direction/reverse
top level: BBa_K266009
start: 1
end: 1414
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267641
display id: annotation2267641
version: 1
top level: BBa_K266009
location: https://synbiohub.org/public/igem/BBa_K266009/annotation2267641/range2267641/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K266009/annotation2267641/range2267641/1
type: Range
persistent identity: range2267641
display id: range2267641
version: 1
direction: direction/reverse
top level: BBa_K266009
start: 1423
end: 1434
orientation: inline

BBa_K112701
type: ComponentDefinition
persistent identity: BBa K112701
display id: BBa_K112701
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hns promoter
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Phns in BBb1 format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of [<Phns>] Basic Part K112701 PCR dv001/dv006 on Phns from E. coli K12 MG1655 (~342 bp, gp = A) PCR dv005/dv003 on Phns from E. coli K12 MG1655 (225 bp, gp = B) PCR dv004/dv002 on Phns from E. coli K12 MG1655 (~149 bp, gp = C) --------------------------------------------------- PCR dv001/dv003 on A+B (355 bp, gp = D) --------------------------------------------------- PCR dv001/dv002 on C+D (697bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+697) Product is pBca1256-K112701 ----------------------------------------------- dv001 Forward Biobricking of [<Phns>] CgATAgaattcATGagatctGAAATATAGCTGTGCCATCAGCC dv002 Reverse Biobricking of [<Phns>] cagtcggatccGCACGAAGAGTACGGATG dv003 Removing the 2nd EcoRI site in [<Phns>] (R) GCCAGGAATGTAAGGgATTCAAAATTGTTC dv004 Removing the 2ND EcoRI site in [<Phns>] (F) GAACAATTTTGAATcCCTTACATTCCTGGC dv005 Removing the FIRST EcoRI site in [<Phns>] (F)CGCTTAATAGGGgATTCTCGTAAACAC dv006 Removing the FIRST EcoRI site in [<Phns>] (R)GTGTTTACGAGAATcCCCTATTAAGCG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112701_sequence/1

BBa_K091110
type: ComponentDefinition
persistent identity: BBa K091110
display id: BBa_K091110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI Promoter
creator: Robert Cool
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified version of the LacI promoter that should bind LacI tighter than the original.
mutable notes: no special considerations
mutable provenance: This promoter is a modified version of the LacI promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091110_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1964082
display id: annotation1964082
version: 1
top level: BBa_K091110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091110/annotation1964082/range1964082/1

https://synbiohub.org/public/igem/BBa_K091110/annotation1964082/range1964082/1
type: Range
persistent identity: range1964082
display id: range1964082
version: 1
direction: direction/forward
top level: BBa_K091110
start: 44
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1964081
display id: annotation1964081
version: 1
top level: BBa_K091110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091110/annotation1964081/range1964081/1

https://synbiohub.org/public/igem/BBa_K091110/annotation1964081/range1964081/1
type: Range
persistent identity: range1964081
display id: range1964081
version: 1
direction: direction/forward
top level: BBa_K091110
start: 17
end: 22
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732436
display id: BBa_I732436
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732436_sequence/1

BBa_K090501
type: ComponentDefinition
persistent identity: BBa K090501
display id: BBa_K090501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gram-Positive IPTG-Inducible Promoter
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is an IPTG-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.
mutable notes: We found the functional annotation in the associated paper: Kaltwasser M., T. Wiegert, and W. Schumann. 2002. Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis. Appl. Environ. Microbiol 68:2624-2628 The primers we used to extract it (sans biobrick ends) are as follow: Forward: AGAACAACCTCTGCTAAAATTC Reverse: AAGCTTAATTGTTATCCGCTC
mutable provenance: We PCRed this part out of the pMUTIN-YFP gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE151.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacOid Operator
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K090501_sequence/1

lacOid Operator
type: SequenceAnnotation
persistent identity: annotation1991824
display id: annotation1991824
version: 1
top level: BBa_K090501
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991824/range1991824/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991824/range1991824/1
type: Range
persistent identity: range1991824
display id: range1991824
version: 1
direction: direction/forward
top level: BBa_K090501
start: 78
end: 97
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1991822
display id: annotation1991822
version: 1
top level: BBa_K090501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991822/range1991822/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991822/range1991822/1
type: Range
persistent identity: range1991822
display id: range1991822
version: 1
direction: direction/forward
top level: BBa_K090501
start: 43
end: 48
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1991823
display id: annotation1991823
version: 1
top level: BBa_K090501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991823/range1991823/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991823/range1991823/1
type: Range
persistent identity: range1991823
display id: range1991823
version: 1
direction: direction/forward
top level: BBa_K090501
start: 66
end: 71
orientation: inline

BBa_K950003
type: ComponentDefinition
persistent identity: BBa K950003
display id: BBa_K950003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast suc2 promotor
creator: Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-09-25T08:34:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1218_
m user id: 26366
owner id: 13191
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Psuc2 promotor from yeast. Repressed by mig1
mutable notes: no restriction sites detected
mutable provenance: yeast genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mig1A_BS
sequence annotation: Mig1B_BS
sequence: https://synbiohub.org/public/igem/BBa_K950003_sequence/1

Mig1A_BS
type: SequenceAnnotation
persistent identity: annotation2179271
display id: annotation2179271
version: 1
top level: BBa_K950003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K950003/annotation2179271/range2179271/1

https://synbiohub.org/public/igem/BBa_K950003/annotation2179271/range2179271/1
type: Range
persistent identity: range2179271
display id: range2179271
version: 1
direction: direction/forward
top level: BBa_K950003
start: 200
end: 213
orientation: inline

Mig1B_BS
type: SequenceAnnotation
persistent identity: annotation2179272
display id: annotation2179272
version: 1
top level: BBa_K950003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K950003/annotation2179272/range2179272/1

https://synbiohub.org/public/igem/BBa_K950003/annotation2179272/range2179272/1
type: Range
persistent identity: range2179272
display id: range2179272
version: 1
direction: direction/forward
top level: BBa_K950003
start: 250
end: 268
orientation: inline

BBa_K121011
type: ComponentDefinition
persistent identity: BBa K121011
display id: BBa_K121011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated)
creator: Masashi Kajita
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
group u list: _220_
m user id: 0
owner id: 3180
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pressure inducible lac promoter
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: K121011
sequence annotation: CAP binding site
sequence annotation: LacI binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K121011_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1981861
display id: annotation1981861
version: 1
top level: BBa_K121011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1
type: Range
persistent identity: range1981861
display id: range1981861
version: 1
direction: direction/forward
top level: BBa_K121011
start: 165
end: 168
orientation: inline

K121011
type: SequenceAnnotation
persistent identity: annotation1981858
display id: annotation1981858
version: 1
top level: BBa_K121011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1
type: Range
persistent identity: range1981858
display id: range1981858
version: 1
direction: direction/forward
top level: BBa_K121011
start: 1
end: 232
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1981859
display id: annotation1981859
version: 1
top level: BBa_K121011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1
type: Range
persistent identity: range1981859
display id: range1981859
version: 1
direction: direction/forward
top level: BBa_K121011
start: 94
end: 131
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1981862
display id: annotation1981862
version: 1
top level: BBa_K121011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1
type: Range
persistent identity: range1981862
display id: range1981862
version: 1
direction: direction/forward
top level: BBa_K121011
start: 169
end: 203
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1981860
display id: annotation1981860
version: 1
top level: BBa_K121011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1
type: Range
persistent identity: range1981860
display id: range1981860
version: 1
direction: direction/forward
top level: BBa_K121011
start: 141
end: 146
orientation: inline

BBa_K1682003
type: ComponentDefinition
persistent identity: BBa K1682003
display id: BBa_K1682003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>C]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:16:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>C]
mutable notes: kdpFp[-15, T>C]
mutable provenance: kdpFp[-15, T>C]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: EcoRI Illegal site removed T->C
sequence annotation: -10
sequence annotation: phospho-KdpE binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K1682003_sequence/1

EcoRI Illegal site removed T->C
type: SequenceAnnotation
persistent identity: annotation2475214
display id: annotation2475214
version: 1
top level: BBa_K1682003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2475214/range2475214/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2475214/range2475214/1
type: Range
persistent identity: range2475214
display id: range2475214
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 65
end: 66
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473589
display id: annotation2473589
version: 1
top level: BBa_K1682003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473589/range2473589/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473589/range2473589/1
type: Range
persistent identity: range2473589
display id: range2473589
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 69
end: 74
orientation: inline

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473591
display id: annotation2473591
version: 1
top level: BBa_K1682003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473591/range2473591/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473591/range2473591/1
type: Range
persistent identity: range2473591
display id: range2473591
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 8
end: 24
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473590
display id: annotation2473590
version: 1
top level: BBa_K1682003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473590/range2473590/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473590/range2473590/1
type: Range
persistent identity: range2473590
display id: range2473590
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 42
end: 47
orientation: inline

luxR
type: ComponentDefinition
persistent identity: BBa R0061
display id: BBa_R0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (HSL-mediated luxR repressor)
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-28T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: Lux Box
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0061_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation305863
display id: annotation305863
version: 1
top level: luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1
type: Range
persistent identity: range305863
display id: range305863
version: 1
direction: direction/forward
top level: luxR
start: 1
end: 6
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation305877
display id: annotation305877
version: 1
top level: luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1
type: Range
persistent identity: range305877
display id: range305877
version: 1
direction: direction/forward
top level: luxR
start: 6
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation305864
display id: annotation305864
version: 1
top level: luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1
type: Range
persistent identity: range305864
display id: range305864
version: 1
direction: direction/forward
top level: luxR
start: 25
end: 30
orientation: inline