//regulation/unknown
type: Collection
persistent identity: regulation unknown
display id: regulation_unknown
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_I723011
member: pFe
member: BBa_K108016
member: BBa_K110007
member: BBa_K165035
member: BBa_J64931
member: BBa_K089006
member: BBa_I764001
member: BBa_K165039
member: PrFe
member: BBa_K110009
member: BBa_J49000
member: BBa_K149001
member: BBa_J16400
member: BBa_K094002
member: BBa_K165015
member: BBa_K110008
member: Pr
member: BBa_J54110
member: BBa_K112126
member: BBa_K165036
member: BBa_J61045
member: BBa_K108014
member: BBa_I735010
member: BBa_K165032
member: BBa_K165040
member: BBa_K165016
member: TFBS
member: BBa_K112407
member: BBa_K165033
member: BBa_I723013
member: BBa_J24001
member: BBa_J48103
member: BBa_I735008
member: pTEF
member: BBa_K089005
member: BBa_K165042
member: BBa_J24000
member: BBa_J48106
member: BBa_I735009
member: BBa_I748001
member: BBa_K108025
member: BBa_K165038

BBa_J61045
type: ComponentDefinition
persistent identity: BBa J61045
display id: BBa_J61045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [spv] spv operon (PoPS out)
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: bob
mutable notes: bob
mutable provenance: bob
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61045_sequence/1

BBa_K165040
type: ComponentDefinition
persistent identity: BBa K165040
display id: BBa_K165040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + TEF constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 transcription factor binding sites on the TEF2 constitutive promoter. Transcription regulation should be constitutive plus regulated by Gli1-binding transcription factors.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165037
sequence annotation: BBa_K165012
sequence annotation: BBa_K165037
sequence: https://synbiohub.org/public/igem/BBa_K165040_sequence/1

BBa_K165012
type: Component
persistent identity: component1996555
display id: component1996555
version: 1
top level: BBa_K165040
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165037
type: Component
persistent identity: component1996556
display id: component1996556
version: 1
top level: BBa_K165040
access: public
definition: pTEF

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1996555
display id: annotation1996555
version: 1
top level: BBa_K165040
location: https://synbiohub.org/public/igem/BBa_K165040/annotation1996555/range1996555/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165040/annotation1996555/range1996555/1
type: Range
persistent identity: range1996555
display id: range1996555
version: 1
direction: direction/reverse
top level: BBa_K165040
start: 1
end: 127
orientation: inline

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1996556
display id: annotation1996556
version: 1
top level: BBa_K165040
location: https://synbiohub.org/public/igem/BBa_K165040/annotation1996556/range1996556/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165040/annotation1996556/range1996556/1
type: Range
persistent identity: range1996556
display id: range1996556
version: 1
direction: direction/reverse
top level: BBa_K165040
start: 136
end: 538
orientation: inline

BBa_J24000
type: ComponentDefinition
persistent identity: BBa J24000
display id: BBa_J24000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CafAp (Cafeine Dependant promoter)
creator: John Cumbers
created: 2006-06-13T11:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 604
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Stimulated by CafA found in cafein addicted students
mutable notes: Also activated by sucrose Inhibited by nicotine
mutable provenance: Student genome sequencing project
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BioBrick
sequence annotation: strong
sequence: https://synbiohub.org/public/igem/BBa_J24000_sequence/1

BioBrick
type: SequenceAnnotation
persistent identity: annotation1880714
display id: annotation1880714
version: 1
top level: BBa_J24000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J24000/annotation1880714/range1880714/1

https://synbiohub.org/public/igem/BBa_J24000/annotation1880714/range1880714/1
type: Range
persistent identity: range1880714
display id: range1880714
version: 1
direction: direction/reverse
top level: BBa_J24000
start: 1
end: 10
orientation: inline

strong
type: SequenceAnnotation
persistent identity: annotation1880709
display id: annotation1880709
version: 1
top level: BBa_J24000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J24000/annotation1880709/range1880709/1

https://synbiohub.org/public/igem/BBa_J24000/annotation1880709/range1880709/1
type: Range
persistent identity: range1880709
display id: range1880709
version: 1
direction: direction/reverse
top level: BBa_J24000
start: 1
end: 13
orientation: inline

BBa_K108014
type: ComponentDefinition
persistent identity: BBa K108014
display id: BBa_K108014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PR
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: promoter of Alcaligenes eutrophus PhaR gene
mutable notes: PCR using primers with biobrick prefix and surfix, cloned into pSB1AC3, and then eliminate Pst1 using site-directed mutation.
mutable provenance: Alcaligenes eutrophus H16 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PR
sequence: https://synbiohub.org/public/igem/BBa_K108014_sequence/1

PR
type: SequenceAnnotation
persistent identity: annotation1986959
display id: annotation1986959
version: 1
top level: BBa_K108014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K108014/annotation1986959/range1986959/1

https://synbiohub.org/public/igem/BBa_K108014/annotation1986959/range1986959/1
type: Range
persistent identity: range1986959
display id: range1986959
version: 1
direction: direction/forward
top level: BBa_K108014
start: 1
end: 234
orientation: inline

BBa_K165033
type: ComponentDefinition
persistent identity: BBa K165033
display id: BBa_K165033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YY1 binding sites + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The YY1 transcription factor binding sites on the mCYC minimal promoter. All transcription regulation comes from YY1-binding transcription factors.
mutable notes: -
mutable provenance: Made in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165013
component: BBa_K165016
sequence annotation: BBa_K165016
sequence annotation: BBa_K165013
sequence: https://synbiohub.org/public/igem/BBa_K165033_sequence/1

BBa_K165013
type: Component
persistent identity: component1995402
display id: component1995402
version: 1
top level: BBa_K165033
access: public
definition: https://synbiohub.org/public/igem/BBa_K165013/1

BBa_K165016
type: Component
persistent identity: component1995403
display id: component1995403
version: 1
top level: BBa_K165033
access: public
definition: BBa_K165016

BBa_K165016
type: SequenceAnnotation
persistent identity: annotation1995403
display id: annotation1995403
version: 1
top level: BBa_K165033
location: https://synbiohub.org/public/igem/BBa_K165033/annotation1995403/range1995403/1
component: BBa_K165016

https://synbiohub.org/public/igem/BBa_K165033/annotation1995403/range1995403/1
type: Range
persistent identity: range1995403
display id: range1995403
version: 1
direction: direction/reverse
top level: BBa_K165033
start: 60
end: 304
orientation: inline

BBa_K165013
type: SequenceAnnotation
persistent identity: annotation1995402
display id: annotation1995402
version: 1
top level: BBa_K165033
location: https://synbiohub.org/public/igem/BBa_K165033/annotation1995402/range1995402/1
component: BBa_K165013

https://synbiohub.org/public/igem/BBa_K165033/annotation1995402/range1995402/1
type: Range
persistent identity: range1995402
display id: range1995402
version: 1
direction: direction/reverse
top level: BBa_K165033
start: 1
end: 51
orientation: inline

BBa_J49000
type: ComponentDefinition
persistent identity: BBa J49000
display id: BBa_J49000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Roid Rage
creator: Andrew Sichel
created: 2006-06-14T11:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
group u list: _74_
m user id: 0
owner id: 1008
owning group id: 74
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Anger and ACNE
mutable notes: needs to be injected
mutable provenance: a sketchy alley
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J49000_sequence/1

BBa_I735008
type: ComponentDefinition
persistent identity: BBa I735008
display id: BBa_I735008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ORE1X Oleate response element
creator: Giovanni Russo
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _139_
m user id: 0
owner id: 1571
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Ore is a consensus sequence localized in some promoters that respond to oleic acid concentration. Its sequence is CGGN3TNA/(R)N8-12CCG
mutable notes: No considerations!
mutable provenance: We amplificated this part from p2UG2XORE plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I735008_sequence/1

BBa_K165032
type: ComponentDefinition
persistent identity: BBa K165032
display id: BBa_K165032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus Gli1 binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Gli1 binding domain. This part contains the sequences for BBa_K165012 and BBa_K165016 but also has some additional DNA.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
mutable provenance: Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165032_sequence/1

BBa_K112126
type: ComponentDefinition
persistent identity: BBa K112126
display id: BBa_K112126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAH57 promoter
creator: Christina Brown
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] <!-- Add more about the biology of this part here
mutable notes: none
mutable provenance: cloned from E. coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112126_sequence/1

BBa_K110009
type: ComponentDefinition
persistent identity: BBa K110009
display id: BBa_K110009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter STE2
creator: Rick Carrick, Ambhi Ganesan, Alex McMillan
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This promotor is on the crick strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: Ste2 Promoter
sequence annotation: STE12
sequence annotation: Mat Alpha2
sequence: https://synbiohub.org/public/igem/BBa_K110009_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1981500
display id: annotation1981500
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981500/range1981500/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981500/range1981500/1
type: Range
persistent identity: range1981500
display id: range1981500
version: 1
direction: direction/forward
top level: BBa_K110009
start: 283
end: 292
orientation: inline

Ste2 Promoter
type: SequenceAnnotation
persistent identity: annotation1981498
display id: annotation1981498
version: 1
top level: BBa_K110009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981498/range1981498/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981498/range1981498/1
type: Range
persistent identity: range1981498
display id: range1981498
version: 1
direction: direction/forward
top level: BBa_K110009
start: 1
end: 501
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981502
display id: annotation1981502
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981502/range1981502/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981502/range1981502/1
type: Range
persistent identity: range1981502
display id: range1981502
version: 1
direction: direction/forward
top level: BBa_K110009
start: 304
end: 312
orientation: inline

Mat Alpha2
type: SequenceAnnotation
persistent identity: annotation1981501
display id: annotation1981501
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981501/range1981501/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981501/range1981501/1
type: Range
persistent identity: range1981501
display id: range1981501
version: 1
direction: direction/forward
top level: BBa_K110009
start: 295
end: 301
orientation: inline

Mat Alpha2
type: SequenceAnnotation
persistent identity: annotation1981499
display id: annotation1981499
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981499/range1981499/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981499/range1981499/1
type: Range
persistent identity: range1981499
display id: range1981499
version: 1
direction: direction/forward
top level: BBa_K110009
start: 273
end: 280
orientation: inline

PrFe
type: ComponentDefinition
persistent identity: BBa J3902
display id: BBa_J3902
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PrFe (PI + PII rus operon)
creator: Latin American iGEM team
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _67_
m user id: 0
owner id: 1000
owning group id: 67
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter region from Acidithiobacillus ferrooxidans rus operon
mutable notes: None
mutable provenance: Sequence found at NCBI (AJ006456)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J3902_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1908375
display id: annotation1908375
version: 1
top level: PrFe
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J3902/annotation1908375/range1908375/1

https://synbiohub.org/public/igem/BBa_J3902/annotation1908375/range1908375/1
type: Range
persistent identity: range1908375
display id: range1908375
version: 1
direction: direction/forward
top level: PrFe
start: 7
end: 266
orientation: inline

BBa_J64931
type: ComponentDefinition
persistent identity: BBa J64931
display id: BBa_J64931
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glnKp promoter
creator: Kristopher Kuchenbecker
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Natural promoter for glnK (-150 to 0)
mutable notes: n/a
mutable provenance: ''E. coli'' K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J64931_sequence/1

BBa_I735009
type: ComponentDefinition
persistent identity: BBa I735009
display id: BBa_I735009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ORE2X oleate response element
creator: Giovanni Russo
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _139_
m user id: 0
owner id: 1571
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Ore is a consensus sequence localized in some promoters that respond to oleic acid concentration. Its sequence is CGGN3TNA/(R)N8-12CCG. In this case we have 2 ore consensus sequences.
mutable notes: Downstream to ORE sequence there is CYC TATA
mutable provenance: We amplified this part from p2UG2XORE plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I735009_sequence/1

BBa_I723011
type: ComponentDefinition
persistent identity: BBa I723011
display id: BBa_I723011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pDntR (estimated promoter for DntR)
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring (possibly stationary-phase only) promoter for the DntR gene (which encodes the transcriptional regulator DntR) back-to-back with the DntR-regulated promoter.
mutable notes: This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
mutable provenance: Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pDntR
sequence: https://synbiohub.org/public/igem/BBa_I723011_sequence/1

pDntR
type: SequenceAnnotation
persistent identity: annotation1955001
display id: annotation1955001
version: 1
top level: BBa_I723011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I723011/annotation1955001/range1955001/1

https://synbiohub.org/public/igem/BBa_I723011/annotation1955001/range1955001/1
type: Range
persistent identity: range1955001
display id: range1955001
version: 1
direction: direction/reverse
top level: BBa_I723011
start: 1
end: 26
orientation: inline

BBa_K165015
type: ComponentDefinition
persistent identity: BBa K165015
display id: BBa_K165015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pADH1 yeast constituative promoter
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a low-level constitutive yeast promoter.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165015_sequence/1

BBa_K108025
type: ComponentDefinition
persistent identity: BBa K108025
display id: BBa_K108025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pu
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: coming...
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: pWW0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pu
sequence: https://synbiohub.org/public/igem/BBa_K108025_sequence/1

Pu
type: SequenceAnnotation
persistent identity: annotation1986980
display id: annotation1986980
version: 1
top level: BBa_K108025
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K108025/annotation1986980/range1986980/1

https://synbiohub.org/public/igem/BBa_K108025/annotation1986980/range1986980/1
type: Range
persistent identity: range1986980
display id: range1986980
version: 1
direction: direction/forward
top level: BBa_K108025
start: 1
end: 200
orientation: inline

BBa_K108016
type: ComponentDefinition
persistent identity: BBa K108016
display id: BBa_K108016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PP
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: promoter of Alcaligenes eutrophus PhaP gene
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: Alcaligenes eutrophus H16 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PP
sequence: https://synbiohub.org/public/igem/BBa_K108016_sequence/1

PP
type: SequenceAnnotation
persistent identity: annotation1986961
display id: annotation1986961
version: 1
top level: BBa_K108016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K108016/annotation1986961/range1986961/1

https://synbiohub.org/public/igem/BBa_K108016/annotation1986961/range1986961/1
type: Range
persistent identity: range1986961
display id: range1986961
version: 1
direction: direction/forward
top level: BBa_K108016
start: 1
end: 406
orientation: inline

BBa_J54110
type: ComponentDefinition
persistent identity: BBa J54110
display id: BBa_J54110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MelR_regulated promoter
creator: Yusaku Nakashima
created: 2006-10-20T11:00:00Z
modified: 2015-08-31T03:48:11Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: promoter
mutable notes: as MelR promoter
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: MelR_regulated promoter
sequence: https://synbiohub.org/public/igem/BBa_J54110_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1903810
display id: annotation1903810
version: 1
top level: BBa_J54110
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54110/annotation1903810/range1903810/1

https://synbiohub.org/public/igem/BBa_J54110/annotation1903810/range1903810/1
type: Range
persistent identity: range1903810
display id: range1903810
version: 1
direction: direction/forward
top level: BBa_J54110
start: 1
end: 5
orientation: inline

MelR_regulated promoter
type: SequenceAnnotation
persistent identity: annotation1903811
display id: annotation1903811
version: 1
top level: BBa_J54110
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J54110/annotation1903811/range1903811/1

https://synbiohub.org/public/igem/BBa_J54110/annotation1903811/range1903811/1
type: Range
persistent identity: range1903811
display id: range1903811
version: 1
direction: direction/forward
top level: BBa_J54110
start: 6
end: 74
orientation: inline

BBa_I748001
type: ComponentDefinition
persistent identity: BBa I748001
display id: BBa_I748001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Putative Cyanide Nitrilase Promoter
creator: Kurt Whittemore
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
group u list: _149_
m user id: 0
owner id: 1654
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This section of DNA contains the sequence from 300 base pairs upstream of the Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase gene to 30 base pairs downstream into the gene from Pseudomonas fluorescens PfO-1. Our hope is that this region of DNA will contain the promoter for the nitrilase gene. At the time that this part was submitted, it was not tested or sequenced yet.
mutable notes: We isolated this segment of DNA from the Pseudomonas fluorescens PfO-1 genome using a PCR reaction.
mutable provenance: This section of DNA comes from the Pseudomonas fluorescens PfO-1 genome (NC_007492). The GeneID for the nitrilase gene that we working with is 3715134, and the protein ID for the nitrilase gene is YP_349417.1. We took the region 300 base pairs upstream of the nitrilase gene and 10 base pairs downstream into the gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I748001_sequence/1

BBa_K165016
type: ComponentDefinition
persistent identity: BBa K165016
display id: BBa_K165016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC1 minimal yeast promoter
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This minimal promoter can be used as a base for designing promoters by adding binding sites upstream.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165016_sequence/1

BBa_K110007
type: ComponentDefinition
persistent identity: BBa K110007
display id: BBa_K110007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA2
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA2 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This gene is on the Watson strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110007_sequence/1

BBa_K089006
type: ComponentDefinition
persistent identity: BBa K089006
display id: BBa_K089006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -663 to Tc start site of phaC
creator: Trent Mortensen
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _245_
m user id: 0
owner id: 3156
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -663 to Tc start site of phaC
mutable notes: -663 to Tc start site of phaC
mutable provenance: Ralsotnia eutropha genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 site
sequence annotation: -10 site
sequence annotation: +1 site
sequence: https://synbiohub.org/public/igem/BBa_K089006_sequence/1

-35 site
type: SequenceAnnotation
persistent identity: annotation1996729
display id: annotation1996729
version: 1
top level: BBa_K089006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089006/annotation1996729/range1996729/1

https://synbiohub.org/public/igem/BBa_K089006/annotation1996729/range1996729/1
type: Range
persistent identity: range1996729
display id: range1996729
version: 1
direction: direction/forward
top level: BBa_K089006
start: 322
end: 327
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1996728
display id: annotation1996728
version: 1
top level: BBa_K089006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089006/annotation1996728/range1996728/1

https://synbiohub.org/public/igem/BBa_K089006/annotation1996728/range1996728/1
type: Range
persistent identity: range1996728
display id: range1996728
version: 1
direction: direction/forward
top level: BBa_K089006
start: 346
end: 351
orientation: inline

+1 site
type: SequenceAnnotation
persistent identity: annotation1996727
display id: annotation1996727
version: 1
top level: BBa_K089006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089006/annotation1996727/range1996727/1

https://synbiohub.org/public/igem/BBa_K089006/annotation1996727/range1996727/1
type: Range
persistent identity: range1996727
display id: range1996727
version: 1
direction: direction/forward
top level: BBa_K089006
start: 357
end: 357
orientation: inline

BBa_K110008
type: ComponentDefinition
persistent identity: BBa K110008
display id: BBa_K110008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA1
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This gene is on the Watson strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MATalpha2
sequence annotation: STE12
sequence annotation: MFA1 Promoter
sequence annotation: MCM1
sequence: https://synbiohub.org/public/igem/BBa_K110008_sequence/1

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981089
display id: annotation1981089
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981089/range1981089/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981089/range1981089/1
type: Range
persistent identity: range1981089
display id: range1981089
version: 1
direction: direction/forward
top level: BBa_K110008
start: 289
end: 297
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981090
display id: annotation1981090
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981090/range1981090/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981090/range1981090/1
type: Range
persistent identity: range1981090
display id: range1981090
version: 1
direction: direction/forward
top level: BBa_K110008
start: 302
end: 309
orientation: inline

MFA1 Promoter
type: SequenceAnnotation
persistent identity: annotation1981086
display id: annotation1981086
version: 1
top level: BBa_K110008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981086/range1981086/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981086/range1981086/1
type: Range
persistent identity: range1981086
display id: range1981086
version: 1
direction: direction/forward
top level: BBa_K110008
start: 1
end: 501
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981087
display id: annotation1981087
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981087/range1981087/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981087/range1981087/1
type: Range
persistent identity: range1981087
display id: range1981087
version: 1
direction: direction/forward
top level: BBa_K110008
start: 267
end: 276
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981088
display id: annotation1981088
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981088/range1981088/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981088/range1981088/1
type: Range
persistent identity: range1981088
display id: range1981088
version: 1
direction: direction/forward
top level: BBa_K110008
start: 277
end: 287
orientation: inline

TFBS
type: ComponentDefinition
persistent identity: BBa K2127006
display id: BBa_K2127006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transcription Factor Binding Site from Cyanobacterial Pcpc560
creator: Mirat Sojitra
created: 2016-10-13T11:00:00Z
modified: 2016-10-25T10:25:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2596_
m user id: 32415
owner id: 32736
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Pcpc560-TFBS
mutable notes: Pcpc560-TFBS
mutable provenance: Pcpc560-TFBS
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Transcription Factor Binding Sites
sequence: https://synbiohub.org/public/igem/BBa_K2127006_sequence/1

Transcription Factor Binding Sites
type: SequenceAnnotation
persistent identity: annotation2530242
display id: annotation2530242
version: 1
top level: TFBS
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2127006/annotation2530242/range2530242/1

https://synbiohub.org/public/igem/BBa_K2127006/annotation2530242/range2530242/1
type: Range
persistent identity: range2530242
display id: range2530242
version: 1
direction: direction/forward
top level: TFBS
start: 1
end: 185
orientation: inline

BBa_J48103
type: ComponentDefinition
persistent identity: BBa J48103
display id: BBa_J48103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Iron promoter
creator: Laina Magaya
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Encodes for oxidoreductase and promotes the reduction/oxidation of iron (FeII and FeII).
mutable notes: Digested using Pst I, Xba I inserted into BBa_E0422.
mutable provenance: Genomic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J48103_sequence/1

BBa_K165035
type: ComponentDefinition
persistent identity: BBa K165035
display id: BBa_K165035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + Zif268-HIV bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 and Zif268-HIV transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and Zif268-HIV binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165030
sequence annotation: BBa_K165030
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165035_sequence/1

BBa_K165012
type: Component
persistent identity: component1995495
display id: component1995495
version: 1
top level: BBa_K165035
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165030
type: Component
persistent identity: component1995497
display id: component1995497
version: 1
top level: BBa_K165035
access: public
definition: https://synbiohub.org/public/igem/BBa_K165030/1

BBa_K165030
type: SequenceAnnotation
persistent identity: annotation1995497
display id: annotation1995497
version: 1
top level: BBa_K165035
location: https://synbiohub.org/public/igem/BBa_K165035/annotation1995497/range1995497/1
component: BBa_K165030

https://synbiohub.org/public/igem/BBa_K165035/annotation1995497/range1995497/1
type: Range
persistent identity: range1995497
display id: range1995497
version: 1
direction: direction/reverse
top level: BBa_K165035
start: 136
end: 442
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995495
display id: annotation1995495
version: 1
top level: BBa_K165035
location: https://synbiohub.org/public/igem/BBa_K165035/annotation1995495/range1995495/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165035/annotation1995495/range1995495/1
type: Range
persistent identity: range1995495
display id: range1995495
version: 1
direction: direction/reverse
top level: BBa_K165035
start: 1
end: 127
orientation: inline

BBa_K165042
type: ComponentDefinition
persistent identity: BBa K165042
display id: BBa_K165042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + MET25 inducible yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165000
sequence annotation: BBa_K165012
sequence annotation: BBa_K165000
sequence: https://synbiohub.org/public/igem/BBa_K165042_sequence/1

BBa_K165012
type: Component
persistent identity: component1996562
display id: component1996562
version: 1
top level: BBa_K165042
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165000
type: Component
persistent identity: component1996563
display id: component1996563
version: 1
top level: BBa_K165042
access: public
definition: https://synbiohub.org/public/igem/BBa_K165000/1

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1996562
display id: annotation1996562
version: 1
top level: BBa_K165042
location: https://synbiohub.org/public/igem/BBa_K165042/annotation1996562/range1996562/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165042/annotation1996562/range1996562/1
type: Range
persistent identity: range1996562
display id: range1996562
version: 1
direction: direction/reverse
top level: BBa_K165042
start: 1
end: 127
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996563
display id: annotation1996563
version: 1
top level: BBa_K165042
location: https://synbiohub.org/public/igem/BBa_K165042/annotation1996563/range1996563/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165042/annotation1996563/range1996563/1
type: Range
persistent identity: range1996563
display id: range1996563
version: 1
direction: direction/reverse
top level: BBa_K165042
start: 136
end: 522
orientation: inline

Pr
type: ComponentDefinition
persistent identity: BBa I723018
display id: BBa_I723018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pr (promoter for XylR)
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring (possibly stationary-phase only) promoter for the XylR gene (which encodes the transcriptional regulator XylR).
mutable notes: [Christine/Maia]
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pr
sequence: https://synbiohub.org/public/igem/BBa_I723018_sequence/1

Pr
type: SequenceAnnotation
persistent identity: annotation1955191
display id: annotation1955191
version: 1
top level: Pr
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I723018/annotation1955191/range1955191/1

https://synbiohub.org/public/igem/BBa_I723018/annotation1955191/range1955191/1
type: Range
persistent identity: range1955191
display id: range1955191
version: 1
direction: direction/forward
top level: Pr
start: 1
end: 410
orientation: inline

BBa_K165039
type: ComponentDefinition
persistent identity: BBa K165039
display id: BBa_K165039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + ADH1 yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Zif268-HIV transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by Zif268-HIV-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165015
component: BBa_K165011
sequence annotation: BBa_K165015
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165039_sequence/1

BBa_K165015
type: Component
persistent identity: component1995517
display id: component1995517
version: 1
top level: BBa_K165039
access: public
definition: BBa_K165015

BBa_K165011
type: Component
persistent identity: component1995516
display id: component1995516
version: 1
top level: BBa_K165039
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1995517
display id: annotation1995517
version: 1
top level: BBa_K165039
location: https://synbiohub.org/public/igem/BBa_K165039/annotation1995517/range1995517/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165039/annotation1995517/range1995517/1
type: Range
persistent identity: range1995517
display id: range1995517
version: 1
direction: direction/reverse
top level: BBa_K165039
start: 55
end: 1499
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1995516
display id: annotation1995516
version: 1
top level: BBa_K165039
location: https://synbiohub.org/public/igem/BBa_K165039/annotation1995516/range1995516/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165039/annotation1995516/range1995516/1
type: Range
persistent identity: range1995516
display id: range1995516
version: 1
direction: direction/reverse
top level: BBa_K165039
start: 1
end: 46
orientation: inline

pTEF
type: ComponentDefinition
persistent identity: BBa K165037
display id: BBa_K165037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEF2 yeast constitutive promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a high level constitutive promoter from the yeast gene TEF2.
mutable notes: -
mutable provenance: From the yeast genome using promoter definition from: Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165037_sequence/1

BBa_J24001
type: ComponentDefinition
persistent identity: BBa J24001
display id: BBa_J24001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: WigLp (Wiggle-dependent Promotor)
creator: Jamie Lemon
created: 2006-06-13T11:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1016
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Is stimulated by wiggle movement.
mutable notes: Is inhibited by lethergy
mutable provenance: Fictional
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: WigLp-a
sequence: https://synbiohub.org/public/igem/BBa_J24001_sequence/1

WigLp-a
type: SequenceAnnotation
persistent identity: annotation1880710
display id: annotation1880710
version: 1
top level: BBa_J24001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J24001/annotation1880710/range1880710/1

https://synbiohub.org/public/igem/BBa_J24001/annotation1880710/range1880710/1
type: Range
persistent identity: range1880710
display id: range1880710
version: 1
direction: direction/forward
top level: BBa_J24001
start: 1
end: 22
orientation: inline

BBa_K165036
type: ComponentDefinition
persistent identity: BBa K165036
display id: BBa_K165036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + LexA bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and LexA-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165031
sequence annotation: BBa_K165012
sequence annotation: BBa_K165031
sequence: https://synbiohub.org/public/igem/BBa_K165036_sequence/1

BBa_K165012
type: Component
persistent identity: component1995510
display id: component1995510
version: 1
top level: BBa_K165036
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165031
type: Component
persistent identity: component1995511
display id: component1995511
version: 1
top level: BBa_K165036
access: public
definition: https://synbiohub.org/public/igem/BBa_K165031/1

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995510
display id: annotation1995510
version: 1
top level: BBa_K165036
location: https://synbiohub.org/public/igem/BBa_K165036/annotation1995510/range1995510/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165036/annotation1995510/range1995510/1
type: Range
persistent identity: range1995510
display id: range1995510
version: 1
direction: direction/reverse
top level: BBa_K165036
start: 1
end: 127
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1995511
display id: annotation1995511
version: 1
top level: BBa_K165036
location: https://synbiohub.org/public/igem/BBa_K165036/annotation1995511/range1995511/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165036/annotation1995511/range1995511/1
type: Range
persistent identity: range1995511
display id: range1995511
version: 1
direction: direction/reverse
top level: BBa_K165036
start: 136
end: 538
orientation: inline

BBa_K149001
type: ComponentDefinition
persistent identity: BBa K149001
display id: BBa_K149001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prp22 promoter
creator: Chris Euler
created: 2008-08-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _243_
m user id: 0
owner id: 3441
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ubiquitous strong promoter.
mutable notes: None
mutable provenance: Simon St. Pierre extracted from S. Cervisiae genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K149001_sequence/1

pFe
type: ComponentDefinition
persistent identity: BBa I765000
display id: BBa_I765000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fe promoter
creator: Raul Cuero
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2266
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter regulated by iron presence
mutable notes: None
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_I765000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1956037
display id: annotation1956037
version: 1
top level: pFe
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I765000/annotation1956037/range1956037/1

https://synbiohub.org/public/igem/BBa_I765000/annotation1956037/range1956037/1
type: Range
persistent identity: range1956037
display id: range1956037
version: 1
direction: direction/forward
top level: pFe
start: 1
end: 1045
orientation: inline

BBa_J16400
type: ComponentDefinition
persistent identity: BBa J16400
display id: BBa_J16400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Iron sensitive promoter (test delete later)
creator: Andrew Hessel
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 341
owning group id: 67
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a promoter that is sensitive to iron (Fe++) concentration (test only)
mutable notes: made a base substitution to remove a EcoRI site
mutable provenance: Sequence obtained from GB accn J00010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: binding region
sequence: https://synbiohub.org/public/igem/BBa_J16400_sequence/1

binding region
type: SequenceAnnotation
persistent identity: annotation1883622
display id: annotation1883622
version: 1
top level: BBa_J16400
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J16400/annotation1883622/range1883622/1

https://synbiohub.org/public/igem/BBa_J16400/annotation1883622/range1883622/1
type: Range
persistent identity: range1883622
display id: range1883622
version: 1
direction: direction/forward
top level: BBa_J16400
start: 11
end: 16
orientation: inline

BBa_K094002
type: ComponentDefinition
persistent identity: BBa K094002
display id: BBa_K094002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plambda P(O-R12)
creator: Shi Lei
created: 2008-07-01T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2053
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: just for testing
mutable notes: just for testing
mutable provenance: just for testing
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K094002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1985278
display id: annotation1985278
version: 1
top level: BBa_K094002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K094002/annotation1985278/range1985278/1

https://synbiohub.org/public/igem/BBa_K094002/annotation1985278/range1985278/1
type: Range
persistent identity: range1985278
display id: range1985278
version: 1
direction: direction/forward
top level: BBa_K094002
start: 1
end: 100
orientation: inline

BBa_I764001
type: ComponentDefinition
persistent identity: BBa I764001
display id: BBa_I764001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ethanol regulated promoter AOX1
creator: Dave Westenberg
created: 2007-08-28T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _137_
m user id: 0
owner id: 1818
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Needs to be added.
mutable notes: No special considerations.
mutable provenance: Pichia pastoris genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: primer_binding
sequence annotation: BioBrick
sequence: https://synbiohub.org/public/igem/BBa_I764001_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation1943750
display id: annotation1943750
version: 1
top level: BBa_I764001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I764001/annotation1943750/range1943750/1

https://synbiohub.org/public/igem/BBa_I764001/annotation1943750/range1943750/1
type: Range
persistent identity: range1943750
display id: range1943750
version: 1
direction: direction/reverse
top level: BBa_I764001
start: 844
end: 867
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation1943749
display id: annotation1943749
version: 1
top level: BBa_I764001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I764001/annotation1943749/range1943749/1

https://synbiohub.org/public/igem/BBa_I764001/annotation1943749/range1943749/1
type: Range
persistent identity: range1943749
display id: range1943749
version: 1
direction: direction/forward
top level: BBa_I764001
start: 1
end: 19
orientation: inline

BioBrick
type: SequenceAnnotation
persistent identity: annotation1943748
display id: annotation1943748
version: 1
top level: BBa_I764001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I764001/annotation1943748/range1943748/1

https://synbiohub.org/public/igem/BBa_I764001/annotation1943748/range1943748/1
type: Range
persistent identity: range1943748
display id: range1943748
version: 1
direction: direction/forward
top level: BBa_I764001
start: 1
end: 867
orientation: inline

BBa_K089005
type: ComponentDefinition
persistent identity: BBa K089005
display id: BBa_K089005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -35 to Tc start site of phaC
creator: Trent Mortensen
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _245_
m user id: 0
owner id: 3156
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -35 to Tc start site of phaC
mutable notes: very small PCR porduct
mutable provenance: Ralstonia eutropha genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1 site
sequence annotation: -35 site
sequence annotation: -10 site
sequence: https://synbiohub.org/public/igem/BBa_K089005_sequence/1

+1 site
type: SequenceAnnotation
persistent identity: annotation1996714
display id: annotation1996714
version: 1
top level: BBa_K089005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089005/annotation1996714/range1996714/1

https://synbiohub.org/public/igem/BBa_K089005/annotation1996714/range1996714/1
type: Range
persistent identity: range1996714
display id: range1996714
version: 1
direction: direction/forward
top level: BBa_K089005
start: 45
end: 45
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1996684
display id: annotation1996684
version: 1
top level: BBa_K089005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089005/annotation1996684/range1996684/1

https://synbiohub.org/public/igem/BBa_K089005/annotation1996684/range1996684/1
type: Range
persistent identity: range1996684
display id: range1996684
version: 1
direction: direction/forward
top level: BBa_K089005
start: 9
end: 14
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1996707
display id: annotation1996707
version: 1
top level: BBa_K089005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089005/annotation1996707/range1996707/1

https://synbiohub.org/public/igem/BBa_K089005/annotation1996707/range1996707/1
type: Range
persistent identity: range1996707
display id: range1996707
version: 1
direction: direction/forward
top level: BBa_K089005
start: 34
end: 39
orientation: inline

BBa_J48106
type: ComponentDefinition
persistent identity: BBa J48106
display id: BBa_J48106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vnfH
creator: Laina Magaya
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Vanadium promoter
mutable notes: cut with Xba I and Pst I
mutable provenance: nitrogenase iron protein, vnfH Rhodopseudomonas palustris CGA009
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J48106_sequence/1

BBa_K112407
type: ComponentDefinition
persistent identity: BBa K112407
display id: BBa_K112407
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for ygeF psuedogene
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. In a Bca1256 plasmid backbone. Our microarray results showed that this gene was upregulated four fold in response to low frequency ultrasound and two fold in response to audible sound. We cloned approximately 400 base pairs upstream from the start codon for the ygeF pseudogene.
mutable notes: none
mutable provenance: Cloned from E. coli MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112407_sequence/1

BBa_K165038
type: ComponentDefinition
persistent identity: BBa K165038
display id: BBa_K165038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + ADH1 constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by YY1-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165015
component: BBa_K165012
sequence annotation: BBa_K165015
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165038_sequence/1

BBa_K165015
type: Component
persistent identity: component1995513
display id: component1995513
version: 1
top level: BBa_K165038
access: public
definition: BBa_K165015

BBa_K165012
type: Component
persistent identity: component1995512
display id: component1995512
version: 1
top level: BBa_K165038
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1995513
display id: annotation1995513
version: 1
top level: BBa_K165038
location: https://synbiohub.org/public/igem/BBa_K165038/annotation1995513/range1995513/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165038/annotation1995513/range1995513/1
type: Range
persistent identity: range1995513
display id: range1995513
version: 1
direction: direction/reverse
top level: BBa_K165038
start: 136
end: 1580
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995512
display id: annotation1995512
version: 1
top level: BBa_K165038
location: https://synbiohub.org/public/igem/BBa_K165038/annotation1995512/range1995512/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165038/annotation1995512/range1995512/1
type: Range
persistent identity: range1995512
display id: range1995512
version: 1
direction: direction/reverse
top level: BBa_K165038
start: 1
end: 127
orientation: inline

BBa_I723013
type: ComponentDefinition
persistent identity: BBa I723013
display id: BBa_I723013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pDntA (estimated promoter for DntA)
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the promoter activated by the transcriptional regulator DntR [BB# here] in response to detection of environmental [salicylate].
mutable notes: '''''This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR'''''. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
mutable provenance: Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pDntA
sequence: https://synbiohub.org/public/igem/BBa_I723013_sequence/1

pDntA
type: SequenceAnnotation
persistent identity: annotation1954960
display id: annotation1954960
version: 1
top level: BBa_I723013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I723013/annotation1954960/range1954960/1

https://synbiohub.org/public/igem/BBa_I723013/annotation1954960/range1954960/1
type: Range
persistent identity: range1954960
display id: range1954960
version: 1
direction: direction/forward
top level: BBa_I723013
start: 1
end: 33
orientation: inline

BBa_I735010
type: ComponentDefinition
persistent identity: BBa I735010
display id: BBa_I735010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This promoter encoding for a thiolase involved in beta-oxidation of fatty acids.
creator: Giovanni Russo
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _139_
m user id: 0
owner id: 1571
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This promoter contains one only ora consensus sequence that respond to oleic acid. Its sequence is CGGN3TNA/(R)N8-12CCG
mutable notes: No considerations!
mutable provenance: We amplified this part from genomic DNA yeast.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I735010_sequence/1