//regulator
type: Collection
persistent identity: regulator
display id: regulator
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1486013
member: BBa_K1315003
member: BBa_K1486000
member: BBa_K1486005
member: BBa_K1486047
member: BBa_K1486048
member: BBa_K1486042
member: BBa_K259005
member: creB
member: BBa_K1486012
member: BBa_K1315007
member: BBa_K1768005
member: BBa_K1165002
member: BBa_K1486049
member: BBa_K2040100
member: BBa_K1486046
member: BBa_K1824012
member: BBa_K2088012
member: BBa_K1486015
member: BBa_K1486050
member: BBa_K1486041
member: BBa_K1486011
member: BBa_K1165003
member: BBa_K259007
member: BBa_K1486002
member: BBa_K772004
member: TFBS
member: Fur
member: BBa_K598001
member: BBa_K2088013
member: BBa_K1486008
member: BBa_K1165004
member: BBa_K174004
member: Nsense
member: BBa_K1486040
member: BBa_K1486010
member: BBa_K1486001
member: BBa_K1486014
member: BBa_K571004
member: BBa_K772005
member: BBa_K1486009
member: BBa_K1486043
member: BBa_K1486056
member: BBa_K2110001
member: BBa_K259013
member: BBa_K606011

BBa_K259013
type: ComponentDefinition
persistent identity: BBa K259013
display id: BBa_K259013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC-RBS-GFP-Terminator
creator: Petros Mina
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A part used to quantify the AraC-RBS construct <partinfo>K259007</partinfo> created by the BCCS-Bristol iGEM'09 team.
mutable notes: RFC10 assembly
mutable provenance: This part was designed with the specified biobricks from the parts registry. AraC occurs naturally in E.coli. GFP occurs in jellyfish species, RBS designed by the Anderson family and Terminator by Resma Shetty.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_B0012
component: BBa_R0081
component: BBa_R0080
component: BBa_B0011
component: BBa_J61100
sequence annotation: BBa_R0081
sequence annotation: BBa_R0080
sequence annotation: BBa_J61100
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K259013_sequence/1

BBa_E0040
type: Component
persistent identity: component2041446
display id: component2041446
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component2041447
display id: component2041447
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0081
type: Component
persistent identity: component2041436
display id: component2041436
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2041443
display id: component2041443
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_B0011
type: Component
persistent identity: component2041451
display id: component2041451
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_J61100
type: Component
persistent identity: component2041444
display id: component2041444
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2041436
display id: annotation2041436
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
component: BBa_R0081

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type: Range
persistent identity: range2041436
display id: range2041436
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 1
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orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2041443
display id: annotation2041443
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
component: BBa_R0080

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type: Range
persistent identity: range2041443
display id: range2041443
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 192
end: 340
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2041444
display id: annotation2041444
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
component: BBa_J61100

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type: Range
persistent identity: range2041444
display id: range2041444
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 349
end: 360
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2041451
display id: annotation2041451
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
component: BBa_B0011

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persistent identity: range2041451
display id: range2041451
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1144
end: 1189
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2041447
display id: annotation2041447
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
component: BBa_B0012

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persistent identity: range2041447
display id: range2041447
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1095
end: 1135
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2041446
display id: annotation2041446
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
component: BBa_E0040

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type: Range
persistent identity: range2041446
display id: range2041446
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 367
end: 1086
orientation: inline

BBa_K1486013
type: ComponentDefinition
persistent identity: BBa K1486013
display id: BBa_K1486013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[2] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[2]. Biobrick BBa_K1486012 has CpxR-IFP[1] with an arabinose promoter. This construct was made in both orientations: BBa_K1486015 is IFP[2]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_K1486000
component: BBa_I0500
component: BBa_K1486007
component: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486007
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486013_sequence/1

BBa_K1486004
type: Component
persistent identity: component2382304
display id: component2382304
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2382303
display id: component2382303
version: 1
top level: BBa_K1486013
access: public
definition: BBa_K1486000

BBa_I0500
type: Component
persistent identity: component2382297
display id: component2382297
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486007
type: Component
persistent identity: component2382308
display id: component2382308
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_B0034
type: Component
persistent identity: component2382299
display id: component2382299
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382304
display id: annotation2382304
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
component: BBa_K1486004

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type: Range
persistent identity: range2382304
display id: range2382304
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1925
end: 1954
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382308
display id: annotation2382308
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
component: BBa_K1486007

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type: Range
persistent identity: range2382308
display id: range2382308
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1955
end: 2533
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382299
display id: annotation2382299
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
component: BBa_B0034

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type: Range
persistent identity: range2382299
display id: range2382299
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382303
display id: annotation2382303
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
type: Range
persistent identity: range2382303
display id: range2382303
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1223
end: 1924
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382297
display id: annotation2382297
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
component: BBa_I0500

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type: Range
persistent identity: range2382297
display id: range2382297
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1
end: 1210
orientation: inline

BBa_K1486048
type: ComponentDefinition
persistent identity: BBa K1486048
display id: BBa_K1486048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR reporter
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Calgary's BBa_K339007 repaired (part didn't have the sequence they said they had.
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_K135000
sequence annotation: BBa_K135000
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K1486048_sequence/1

BBa_I13507
type: Component
persistent identity: component2398423
display id: component2398423
version: 1
top level: BBa_K1486048
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K135000
type: Component
persistent identity: component2398411
display id: component2398411
version: 1
top level: BBa_K1486048
access: public
definition: https://synbiohub.org/public/igem/BBa_K135000/1

BBa_K135000
type: SequenceAnnotation
persistent identity: annotation2398411
display id: annotation2398411
version: 1
top level: BBa_K1486048
location: https://synbiohub.org/public/igem/BBa_K1486048/annotation2398411/range2398411/1
component: BBa_K135000

https://synbiohub.org/public/igem/BBa_K1486048/annotation2398411/range2398411/1
type: Range
persistent identity: range2398411
display id: range2398411
version: 1
direction: direction/reverse
top level: BBa_K1486048
start: 1
end: 55
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398423
display id: annotation2398423
version: 1
top level: BBa_K1486048
location: https://synbiohub.org/public/igem/BBa_K1486048/annotation2398423/range2398423/1
component: BBa_I13507

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type: Range
persistent identity: range2398423
display id: range2398423
version: 1
direction: direction/reverse
top level: BBa_K1486048
start: 64
end: 924
orientation: inline

BBa_K1768005
type: ComponentDefinition
persistent identity: BBa K1768005
display id: BBa_K1768005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Invertible promoter with RFP reporter
creator: Gert Pietersen
created: 2015-09-17T11:00:00Z
modified: 2015-09-25T07:45:33Z
discontinued: false
dominant: false
group u list: _2190_
m user id: 26979
owner id: 26979
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is for the characterisation of the Invertible promoter BBa_K1768001.
mutable notes: NA
mutable provenance: BBa_K1768001 BBa_B0034 BBa_E1010 This part was synthesised by IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1768001
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_K1768001
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1768005_sequence/1

BBa_B0034
type: Component
persistent identity: component2471274
display id: component2471274
version: 1
top level: BBa_K1768005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1768001
type: Component
persistent identity: component2471272
display id: component2471272
version: 1
top level: BBa_K1768005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1768001/1

BBa_E1010
type: Component
persistent identity: component2471277
display id: component2471277
version: 1
top level: BBa_K1768005
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2471277
display id: annotation2471277
version: 1
top level: BBa_K1768005
location: https://synbiohub.org/public/igem/BBa_K1768005/annotation2471277/range2471277/1
component: BBa_E1010

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type: Range
persistent identity: range2471277
display id: range2471277
version: 1
direction: direction/reverse
top level: BBa_K1768005
start: 290
end: 995
orientation: inline

BBa_K1768001
type: SequenceAnnotation
persistent identity: annotation2471272
display id: annotation2471272
version: 1
top level: BBa_K1768005
location: https://synbiohub.org/public/igem/BBa_K1768005/annotation2471272/range2471272/1
component: BBa_K1768001

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type: Range
persistent identity: range2471272
display id: range2471272
version: 1
direction: direction/reverse
top level: BBa_K1768005
start: 1
end: 263
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2471274
display id: annotation2471274
version: 1
top level: BBa_K1768005
location: https://synbiohub.org/public/igem/BBa_K1768005/annotation2471274/range2471274/1
component: BBa_B0034

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type: Range
persistent identity: range2471274
display id: range2471274
version: 1
direction: direction/reverse
top level: BBa_K1768005
start: 272
end: 283
orientation: inline

BBa_K259005
type: ComponentDefinition
persistent identity: BBa K259005
display id: BBa_K259005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Rheostat Promoter
creator: Petros Mina
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the expression of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. This composite part does not have an LVA degradation tag at its end, hence can be used with all prokaryotic assemblies.
mutable notes: The part was designed and assembled from the iGEM2009 kit with standard RFC10 assembly.
mutable provenance: The AraC promoter is found naturally in E.Coli at 1.52 min/centisome in the clockwise orientation. More information can be found at [http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0080
component: BBa_R0081
sequence annotation: BBa_R0081
sequence annotation: BBa_R0080
sequence: https://synbiohub.org/public/igem/BBa_K259005_sequence/1

BBa_R0080
type: Component
persistent identity: component2018294
display id: component2018294
version: 1
top level: BBa_K259005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_R0081
type: Component
persistent identity: component2018287
display id: component2018287
version: 1
top level: BBa_K259005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2018287
display id: annotation2018287
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018287/range2018287/1
component: BBa_R0081

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type: Range
persistent identity: range2018287
display id: range2018287
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 1
end: 183
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2018294
display id: annotation2018294
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018294/range2018294/1
component: BBa_R0080

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type: Range
persistent identity: range2018294
display id: range2018294
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 192
end: 340
orientation: inline

BBa_K1486002
type: ComponentDefinition
persistent identity: BBa K1486002
display id: BBa_K1486002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP CpxR (Nterm)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the N terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486000
component: BBa_K1486003
component: BBa_B0034
component: BBa_I746916
component: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486003
sequence annotation: BBa_I0500
sequence annotation: BBa_I746916
sequence: https://synbiohub.org/public/igem/BBa_K1486002_sequence/1

BBa_K1486000
type: Component
persistent identity: component2382068
display id: component2382068
version: 1
top level: BBa_K1486002
access: public
definition: BBa_K1486000

BBa_K1486003
type: Component
persistent identity: component2382064
display id: component2382064
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_B0034
type: Component
persistent identity: component2382059
display id: component2382059
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I746916
type: Component
persistent identity: component2382063
display id: component2382063
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_I0500
type: Component
persistent identity: component2382057
display id: component2382057
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382059
display id: annotation2382059
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
type: Range
persistent identity: range2382059
display id: range2382059
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382068
display id: annotation2382068
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
type: Range
persistent identity: range2382068
display id: range2382068
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1967
end: 2668
orientation: inline

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382064
display id: annotation2382064
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
component: BBa_K1486003

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
type: Range
persistent identity: range2382064
display id: range2382064
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1943
end: 1966
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382057
display id: annotation2382057
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
type: Range
persistent identity: range2382057
display id: range2382057
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1
end: 1210
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382063
display id: annotation2382063
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
type: Range
persistent identity: range2382063
display id: range2382063
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1223
end: 1942
orientation: inline

BBa_K174004
type: ComponentDefinition
persistent identity: BBa K174004
display id: BBa_K174004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspac promoter
creator: The Newcastle 2009 iGEM team
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:10:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _277_
m user id: 0
owner id: 3942
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter can be induced by IPTG. Wild type LacI binding site is replaced with a perfect palindromic "oid" operator sequence which increases the repression by LacI. This biobrick is an enhancement of Biobrick BBa_K090501 which has the sequence from pmutin2.
mutable notes: pmutin2's genbank accession number is AF072806. Although there is no sequence information for pmutin4, it can be derived from pmutin2's sequence information by changing the O1 lacI binding site with the "oid" sequence.
mutable provenance: This sequence is taken from pmutin4 integration vector. pmutin2's lacI binding site was changed with the "oid" sequence to create pmutin4.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pspac promoter
sequence annotation: oid - Lac operator
sequence: https://synbiohub.org/public/igem/BBa_K174004_sequence/1

pspac promoter
type: SequenceAnnotation
persistent identity: annotation2033094
display id: annotation2033094
version: 1
top level: BBa_K174004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K174004/annotation2033094/range2033094/1

https://synbiohub.org/public/igem/BBa_K174004/annotation2033094/range2033094/1
type: Range
persistent identity: range2033094
display id: range2033094
version: 1
direction: direction/forward
top level: BBa_K174004
start: 43
end: 80
orientation: inline

oid - Lac operator
type: SequenceAnnotation
persistent identity: annotation2033093
display id: annotation2033093
version: 1
top level: BBa_K174004
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K174004/annotation2033093/range2033093/1

https://synbiohub.org/public/igem/BBa_K174004/annotation2033093/range2033093/1
type: Range
persistent identity: range2033093
display id: range2033093
version: 1
direction: direction/forward
top level: BBa_K174004
start: 81
end: 100
orientation: inline

BBa_K1486014
type: ComponentDefinition
persistent identity: BBa K1486014
display id: BBa_K1486014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[1]-CpxR under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[1]-CpxR. Biobrick BBa_K1486015 has IFP[2]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486012 is CpxR-IFP[1] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put between the promoter and the cds.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486006
component: BBa_K1486004
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486000
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486004
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486014_sequence/1

BBa_K1486006
type: Component
persistent identity: component2424742
display id: component2424742
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486004
type: Component
persistent identity: component2424743
display id: component2424743
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_I0500
type: Component
persistent identity: component2424736
display id: component2424736
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2424738
display id: component2424738
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2424747
display id: component2424747
version: 1
top level: BBa_K1486014
access: public
definition: BBa_K1486000

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424742
display id: annotation2424742
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
type: Range
persistent identity: range2424742
display id: range2424742
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1223
end: 1627
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424743
display id: annotation2424743
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
type: Range
persistent identity: range2424743
display id: range2424743
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1628
end: 1657
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424736
display id: annotation2424736
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
type: Range
persistent identity: range2424736
display id: range2424736
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424738
display id: annotation2424738
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
type: Range
persistent identity: range2424738
display id: range2424738
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424747
display id: annotation2424747
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
type: Range
persistent identity: range2424747
display id: range2424747
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1658
end: 2359
orientation: inline

BBa_K606011
type: ComponentDefinition
persistent identity: BBa K606011
display id: BBa_K606011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG/Lactose inducible expression casette for B. Subtilis
creator: Cyrille Pauthenier
created: 2011-07-06T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
group u list: _778_
m user id: 0
owner id: 8998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part in an IPTG/Lactose inducible expression casette for Bacillus Subtilis. It is build to have a slow and controlable response with the quantity of IPTG introduced in the medium.
mutable notes: The experiments have still to be done. The host plasmid is pSB1K3
mutable provenance: This part is the fusion of the biobrick K606010 and K143055
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K606010
component: BBa_K143055
sequence annotation: BBa_K143055
sequence annotation: BBa_K606010
sequence: https://synbiohub.org/public/igem/BBa_K606011_sequence/1

BBa_K606010
type: Component
persistent identity: component2123131
display id: component2123131
version: 1
top level: BBa_K606011
access: public
definition: https://synbiohub.org/public/igem/BBa_K606010/1

BBa_K143055
type: Component
persistent identity: component2123139
display id: component2123139
version: 1
top level: BBa_K606011
access: public
definition: https://synbiohub.org/public/igem/BBa_K143055/1

BBa_K143055
type: SequenceAnnotation
persistent identity: annotation2123139
display id: annotation2123139
version: 1
top level: BBa_K606011
location: https://synbiohub.org/public/igem/BBa_K606011/annotation2123139/range2123139/1
component: BBa_K143055

https://synbiohub.org/public/igem/BBa_K606011/annotation2123139/range2123139/1
type: Range
persistent identity: range2123139
display id: range2123139
version: 1
direction: direction/reverse
top level: BBa_K606011
start: 1355
end: 1475
orientation: inline

BBa_K606010
type: SequenceAnnotation
persistent identity: annotation2123131
display id: annotation2123131
version: 1
top level: BBa_K606011
location: https://synbiohub.org/public/igem/BBa_K606011/annotation2123131/range2123131/1
component: BBa_K606010

https://synbiohub.org/public/igem/BBa_K606011/annotation2123131/range2123131/1
type: Range
persistent identity: range2123131
display id: range2123131
version: 1
direction: direction/reverse
top level: BBa_K606011
start: 1
end: 1346
orientation: inline

BBa_K2110001
type: ComponentDefinition
persistent identity: BBa K2110001
display id: BBa_K2110001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPA inducible Leader sequence (TILS)
creator: Le He
created: 2016-10-04T11:00:00Z
modified: 2016-10-05T07:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2578_
m user id: 32957
owner id: 32957
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This sequence comes from RhodococcusjostiiRHA1. Rhodococcus owns the ability of degrading terephthalic acid (TPA), this pathway is regulated by many genes and some of them can be induced by TPA. The inducing effect is based on a regulatory proteintpaR, which can bind this sequence at the existence of TPA and induce the expression of downstream gene. This sequence should be placed before the sequence of promoter.
mutable notes: This sequence is very short so if the gel recycling is necessary, the agarose concentration should be increased.
mutable provenance: It comes from the genome of RhodococcusjostiiRHA1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BioBrick
sequence: https://synbiohub.org/public/igem/BBa_K2110001_sequence/1

BioBrick
type: SequenceAnnotation
persistent identity: annotation2486062
display id: annotation2486062
version: 1
top level: BBa_K2110001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2110001/annotation2486062/range2486062/1

https://synbiohub.org/public/igem/BBa_K2110001/annotation2486062/range2486062/1
type: Range
persistent identity: range2486062
display id: range2486062
version: 1
direction: direction/forward
top level: BBa_K2110001
start: 1
end: 96
orientation: inline

BBa_K772004
type: ComponentDefinition
persistent identity: BBa K772004
display id: BBa_K772004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL regulated PoPS generator: Las receiver device
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated. This part represents an alternative quorum sensing receiver device, which is able to generate a PoPS response in the presence of 6HSLO3 produced by LasI, instead of BBa_F2620. This will allow iGEMers to establish communications between different bacteria colonies and it makes simple to generate easy regulated complex systems. This part is assembled with Las promoter system to improve the current quorum sensing devices and parts which commonly base on Lux promoter system.
mutable notes: Note that pLas has no RBS afterwards, the assembling methods must be selected after this consideration.
mutable provenance: pLas and LasR sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Double Terminator
sequence annotation: pTetR
sequence annotation: RBS
sequence annotation: pLas
sequence annotation: LasR
sequence: https://synbiohub.org/public/igem/BBa_K772004_sequence/1

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203690
display id: annotation2203690
version: 1
top level: BBa_K772004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203690/range2203690/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203690/range2203690/1
type: Range
persistent identity: range2203690
display id: range2203690
version: 1
direction: direction/forward
top level: BBa_K772004
start: 825
end: 954
orientation: inline

pTetR
type: SequenceAnnotation
persistent identity: annotation2203687
display id: annotation2203687
version: 1
top level: BBa_K772004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203687/range2203687/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203687/range2203687/1
type: Range
persistent identity: range2203687
display id: range2203687
version: 1
direction: direction/forward
top level: BBa_K772004
start: 1
end: 54
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203688
display id: annotation2203688
version: 1
top level: BBa_K772004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203688/range2203688/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203688/range2203688/1
type: Range
persistent identity: range2203688
display id: range2203688
version: 1
direction: direction/forward
top level: BBa_K772004
start: 55
end: 75
orientation: inline

pLas
type: SequenceAnnotation
persistent identity: annotation2203691
display id: annotation2203691
version: 1
top level: BBa_K772004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203691/range2203691/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203691/range2203691/1
type: Range
persistent identity: range2203691
display id: range2203691
version: 1
direction: direction/forward
top level: BBa_K772004
start: 955
end: 1122
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation2203689
display id: annotation2203689
version: 1
top level: BBa_K772004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203689/range2203689/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203689/range2203689/1
type: Range
persistent identity: range2203689
display id: range2203689
version: 1
direction: direction/forward
top level: BBa_K772004
start: 76
end: 824
orientation: inline

BBa_K1486043
type: ComponentDefinition
persistent identity: BBa K1486043
display id: BBa_K1486043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leucine Zipper + split rLuc
creator: EPFL iGem team 2014
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To edit. Pense ?? rajouter les linkers, je ne sais pas s'ils sont de igem ou s'il faut ajouter la s??quence ?? la registry. Check pour tous tes biobricks et dis-moi, please.
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486042
component: BBa_B0034
component: BBa_K1486019
component: BBa_K1486020
component: BBa_I0500
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486042
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486019
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486043_sequence/1

BBa_K1486042
type: Component
persistent identity: component2392177
display id: component2392177
version: 1
top level: BBa_K1486043
access: public
definition: BBa_K1486042

BBa_B0034
type: Component
persistent identity: component2392172
display id: component2392172
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2392176
display id: component2392176
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486019
type: Component
persistent identity: component2392174
display id: component2392174
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K1486020
type: Component
persistent identity: component2392178
display id: component2392178
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_K1486042
type: Component
persistent identity: component2392173
display id: component2392173
version: 1
top level: BBa_K1486043
access: public
definition: BBa_K1486042

BBa_I0500
type: Component
persistent identity: component2392170
display id: component2392170
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2392178
display id: annotation2392178
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
type: Range
persistent identity: range2392178
display id: range2392178
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1865
end: 2470
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392177
display id: annotation2392177
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392177/range2392177/1
component: BBa_K1486042

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392177/range2392177/1
type: Range
persistent identity: range2392177
display id: range2392177
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1715
end: 1864
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392172
display id: annotation2392172
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
type: Range
persistent identity: range2392172
display id: range2392172
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1211
end: 1222
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2392174
display id: annotation2392174
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
type: Range
persistent identity: range2392174
display id: range2392174
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1373
end: 1702
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392176
display id: annotation2392176
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
type: Range
persistent identity: range2392176
display id: range2392176
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1703
end: 1714
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392173
display id: annotation2392173
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
component: BBa_K1486042

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
type: Range
persistent identity: range2392173
display id: range2392173
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1223
end: 1372
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2392170
display id: annotation2392170
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
type: Range
persistent identity: range2392170
display id: range2392170
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1
end: 1210
orientation: inline

BBa_K571004
type: ComponentDefinition
persistent identity: BBa K571004
display id: BBa_K571004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0011+RBS/pSB1C3
creator: Lu-Chu Ke
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _739_
m user id: 0
owner id: 10422
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This biobrick contains the BBa_R0011 promoter of 80 bp cloned in pSB1C3 with EcoRI and XbaI restriction site which will can be induced by IPTG. RBS is added to the end of the sequence in order to enable gene expression to perform well.
mutable notes: none.
mutable provenance: BBa_R0011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RBS
sequence annotation: R0011
sequence: https://synbiohub.org/public/igem/BBa_K571004_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2151465
display id: annotation2151465
version: 1
top level: BBa_K571004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K571004/annotation2151465/range2151465/1

https://synbiohub.org/public/igem/BBa_K571004/annotation2151465/range2151465/1
type: Range
persistent identity: range2151465
display id: range2151465
version: 1
direction: direction/forward
top level: BBa_K571004
start: 64
end: 80
orientation: inline

R0011
type: SequenceAnnotation
persistent identity: annotation2151464
display id: annotation2151464
version: 1
top level: BBa_K571004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K571004/annotation2151464/range2151464/1

https://synbiohub.org/public/igem/BBa_K571004/annotation2151464/range2151464/1
type: Range
persistent identity: range2151464
display id: range2151464
version: 1
direction: direction/forward
top level: BBa_K571004
start: 1
end: 55
orientation: inline

Nsense
type: ComponentDefinition
persistent identity: BBa M36356
display id: BBa_M36356
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A sensor that activates in the presence of nitrate in anaerobic conditions in E. coli.
creator: Ana Ordonez, Chris Harrell
created: 2014-10-22T11:00:00Z
modified: 2015-05-08T01:14:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 24178
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part will used along with a Green fluorescent actuator to test for the presence of nitrate.
mutable notes: We had to deal with finding the appropriate binding areas for the promote without including any of the sequence that codes for proteins.
mutable provenance: The source for this part comes from the NIR promoter region in E. coli plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: NarL/P
sequence annotation: FIS I
sequence annotation: IHF II
sequence annotation: IHF I
sequence annotation: FNR
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_M36356_sequence/1

NarL/P
type: SequenceAnnotation
persistent identity: annotation2428944
display id: annotation2428944
version: 1
top level: Nsense
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_M36356/annotation2428944/range2428944/1

https://synbiohub.org/public/igem/BBa_M36356/annotation2428944/range2428944/1
type: Range
persistent identity: range2428944
display id: range2428944
version: 1
direction: direction/forward
top level: Nsense
start: 74
end: 88
orientation: inline

FIS I
type: SequenceAnnotation
persistent identity: annotation2429019
display id: annotation2429019
version: 1
top level: Nsense
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_M36356/annotation2429019/range2429019/1

https://synbiohub.org/public/igem/BBa_M36356/annotation2429019/range2429019/1
type: Range
persistent identity: range2429019
display id: range2429019
version: 1
direction: direction/forward
top level: Nsense
start: 2
end: 16
orientation: inline

IHF II
type: SequenceAnnotation
persistent identity: annotation2429001
display id: annotation2429001
version: 1
top level: Nsense
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_M36356/annotation2429001/range2429001/1

https://synbiohub.org/public/igem/BBa_M36356/annotation2429001/range2429001/1
type: Range
persistent identity: range2429001
display id: range2429001
version: 1
direction: direction/forward
top level: Nsense
start: 22
end: 48
orientation: inline

IHF I
type: SequenceAnnotation
persistent identity: annotation2429010
display id: annotation2429010
version: 1
top level: Nsense
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_M36356/annotation2429010/range2429010/1

https://synbiohub.org/public/igem/BBa_M36356/annotation2429010/range2429010/1
type: Range
persistent identity: range2429010
display id: range2429010
version: 1
direction: direction/forward
top level: Nsense
start: 49
end: 75
orientation: inline

FNR
type: SequenceAnnotation
persistent identity: annotation2428943
display id: annotation2428943
version: 1
top level: Nsense
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_M36356/annotation2428943/range2428943/1

https://synbiohub.org/public/igem/BBa_M36356/annotation2428943/range2428943/1
type: Range
persistent identity: range2428943
display id: range2428943
version: 1
direction: direction/forward
top level: Nsense
start: 103
end: 116
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation2429611
display id: annotation2429611
version: 1
top level: Nsense
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36356/annotation2429611/range2429611/1

https://synbiohub.org/public/igem/BBa_M36356/annotation2429611/range2429611/1
type: Range
persistent identity: range2429611
display id: range2429611
version: 1
direction: direction/forward
top level: Nsense
start: 150
end: 150
orientation: inline

BBa_K1486011
type: ComponentDefinition
persistent identity: BBa K1486011
display id: BBa_K1486011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486006
component: BBa_K1486000
component: BBa_I0500
component: BBa_K1486007
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486006
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486011_sequence/1

BBa_B0034
type: Component
persistent identity: component2382245
display id: component2382245
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2382250
display id: component2382250
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: Component
persistent identity: component2382243
display id: component2382243
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486004
type: Component
persistent identity: component2382242
display id: component2382242
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2382254
display id: component2382254
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486000

BBa_K1486000
type: Component
persistent identity: component2382241
display id: component2382241
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486000

BBa_I0500
type: Component
persistent identity: component2382235
display id: component2382235
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486007
type: Component
persistent identity: component2382249
display id: component2382249
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_B0034
type: Component
persistent identity: component2382237
display id: component2382237
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382249
display id: annotation2382249
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
type: Range
persistent identity: range2382249
display id: range2382249
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2549
end: 3127
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382243
display id: annotation2382243
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
type: Range
persistent identity: range2382243
display id: range2382243
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1955
end: 2536
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382245
display id: annotation2382245
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
type: Range
persistent identity: range2382245
display id: range2382245
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2537
end: 2548
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382250
display id: annotation2382250
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
type: Range
persistent identity: range2382250
display id: range2382250
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3128
end: 3157
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382237
display id: annotation2382237
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382237/range2382237/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382237/range2382237/1
type: Range
persistent identity: range2382237
display id: range2382237
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382241
display id: annotation2382241
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
type: Range
persistent identity: range2382241
display id: range2382241
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1223
end: 1924
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382242
display id: annotation2382242
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
type: Range
persistent identity: range2382242
display id: range2382242
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1925
end: 1954
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382254
display id: annotation2382254
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
type: Range
persistent identity: range2382254
display id: range2382254
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3158
end: 3859
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382235
display id: annotation2382235
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
type: Range
persistent identity: range2382235
display id: range2382235
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1
end: 1210
orientation: inline

BBa_K2088013
type: ComponentDefinition
persistent identity: BBa K2088013
display id: BBa_K2088013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mhbpt: a promoter that can be induced by 3-HBA and MhbR.
creator: Shi Kejian
created: 2016-10-07T11:00:00Z
modified: 2016-10-14T01:15:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2556_
m user id: 33751
owner id: 29893
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Mhbpt is a promoter that can be induced by the compound of MhbR and the 3-HBA. The promoter contains the binding site to bind the complex. It is a bidirectional promoter for the two promoters have a partial overlap.
mutable notes: None
mutable provenance: The gene can be gotton from the bacteria M5a1, and the sequence comes from GenBank https://www.ncbi.nlm.nih.gov/nuccore/EU165545.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: complex binding site
sequence annotation: Mhbpt right promoter
sequence annotation: Mhbpt left promoter
sequence: https://synbiohub.org/public/igem/BBa_K2088013_sequence/1

complex binding site
type: SequenceAnnotation
persistent identity: annotation2488228
display id: annotation2488228
version: 1
top level: BBa_K2088013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2088013/annotation2488228/range2488228/1

https://synbiohub.org/public/igem/BBa_K2088013/annotation2488228/range2488228/1
type: Range
persistent identity: range2488228
display id: range2488228
version: 1
direction: direction/forward
top level: BBa_K2088013
start: 343
end: 435
orientation: inline

Mhbpt right promoter
type: SequenceAnnotation
persistent identity: annotation2488226
display id: annotation2488226
version: 1
top level: BBa_K2088013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2088013/annotation2488226/range2488226/1

https://synbiohub.org/public/igem/BBa_K2088013/annotation2488226/range2488226/1
type: Range
persistent identity: range2488226
display id: range2488226
version: 1
direction: direction/forward
top level: BBa_K2088013
start: 323
end: 342
orientation: inline

complex binding site
type: SequenceAnnotation
persistent identity: annotation2488227
display id: annotation2488227
version: 1
top level: BBa_K2088013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2088013/annotation2488227/range2488227/1

https://synbiohub.org/public/igem/BBa_K2088013/annotation2488227/range2488227/1
type: Range
persistent identity: range2488227
display id: range2488227
version: 1
direction: direction/reverse
top level: BBa_K2088013
start: 1
end: 302
orientation: inline

Mhbpt left promoter
type: SequenceAnnotation
persistent identity: annotation2488225
display id: annotation2488225
version: 1
top level: BBa_K2088013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2088013/annotation2488225/range2488225/1

https://synbiohub.org/public/igem/BBa_K2088013/annotation2488225/range2488225/1
type: Range
persistent identity: range2488225
display id: range2488225
version: 1
direction: direction/reverse
top level: BBa_K2088013
start: 303
end: 329
orientation: inline

BBa_K1486001
type: ComponentDefinition
persistent identity: BBa K1486001
display id: BBa_K1486001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + CpxR
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The part contains CpxR with an upstream arabinose-inducible promoter.
mutable notes: The arabinose had to be upstream of the CpxR. Also, the sequence had to be tested because of a mutation, and it works perfectly.
mutable provenance: The CpxR comes from the genome of E coli, and the arabinose promoter comes from a biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486000
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486001_sequence/1

BBa_I0500
type: Component
persistent identity: component2382072
display id: component2382072
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2382074
display id: component2382074
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382078
display id: component2382078
version: 1
top level: BBa_K1486001
access: public
definition: BBa_K1486000

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382078
display id: annotation2382078
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
type: Range
persistent identity: range2382078
display id: range2382078
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1223
end: 1924
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382072
display id: annotation2382072
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
type: Range
persistent identity: range2382072
display id: range2382072
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382074
display id: annotation2382074
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
type: Range
persistent identity: range2382074
display id: range2382074
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1211
end: 1222
orientation: inline

BBa_K1486047
type: ComponentDefinition
persistent identity: BBa K1486047
display id: BBa_K1486047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter RV
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1486047_sequence/1

BBa_K2088012
type: ComponentDefinition
persistent identity: BBa K2088012
display id: BBa_K2088012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MhbRpt is a complex promoter which can be induced by 3-HBA.
creator: Shi Kejian
created: 2016-10-07T11:00:00Z
modified: 2016-10-08T10:34:22Z
discontinued: false
dominant: false
group u list: _2556_
m user id: 29893
owner id: 29893
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Besides the MhbR, there is a promoter named Mhbpt which can be induced by the compound of MhbR and the 3-HBA. For there has been the MhbR, only the 3-HBA will induce the complex promoter. It can be used as an induced start system.
mutable notes: One bp of G was mutated to the A to avoid the formation of the PstⅠ.
mutable provenance: The gene can be gotton from the bacteria M5a1, and the sequence comes from GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MhbR coding region
sequence annotation: Mhbpt right promoter
sequence annotation: Mhbpt left promoter
sequence annotation: TGA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K2088012_sequence/1

MhbR coding region
type: SequenceAnnotation
persistent identity: annotation2488219
display id: annotation2488219
version: 1
top level: BBa_K2088012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2088012/annotation2488219/range2488219/1

https://synbiohub.org/public/igem/BBa_K2088012/annotation2488219/range2488219/1
type: Range
persistent identity: range2488219
display id: range2488219
version: 1
direction: direction/reverse
top level: BBa_K2088012
start: 1
end: 918
orientation: inline

Mhbpt right promoter
type: SequenceAnnotation
persistent identity: annotation2488223
display id: annotation2488223
version: 1
top level: BBa_K2088012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2088012/annotation2488223/range2488223/1

https://synbiohub.org/public/igem/BBa_K2088012/annotation2488223/range2488223/1
type: Range
persistent identity: range2488223
display id: range2488223
version: 1
direction: direction/forward
top level: BBa_K2088012
start: 970
end: 989
orientation: inline

Mhbpt left promoter
type: SequenceAnnotation
persistent identity: annotation2488220
display id: annotation2488220
version: 1
top level: BBa_K2088012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2088012/annotation2488220/range2488220/1

https://synbiohub.org/public/igem/BBa_K2088012/annotation2488220/range2488220/1
type: Range
persistent identity: range2488220
display id: range2488220
version: 1
direction: direction/reverse
top level: BBa_K2088012
start: 950
end: 976
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2488224
display id: annotation2488224
version: 1
top level: BBa_K2088012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2088012/annotation2488224/range2488224/1

https://synbiohub.org/public/igem/BBa_K2088012/annotation2488224/range2488224/1
type: Range
persistent identity: range2488224
display id: range2488224
version: 1
direction: direction/reverse
top level: BBa_K2088012
start: 1
end: 3
orientation: inline

BBa_K598001
type: ComponentDefinition
persistent identity: BBa K598001
display id: BBa_K598001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator
creator: Siyang HAO, Tong MU, Zheng LUO, Mengqi ZHANG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to regulate the translation of other genes.
mutable notes: The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well.
mutable provenance: Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: BBa_E0040
sequence annotation: T7 TE
sequence annotation: Engineered RBS
sequence annotation: Theophylline responsive riboswitch Theo 1G1
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: eGFP
sequence annotation: BBa_B0012
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_K598001_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2152892
display id: annotation2152892
version: 1
top level: BBa_K598001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152892/range2152892/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152892/range2152892/1
type: Range
persistent identity: range2152892
display id: range2152892
version: 1
direction: direction/forward
top level: BBa_K598001
start: 936
end: 936
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2152703
display id: annotation2152703
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152703/range2152703/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152703/range2152703/1
type: Range
persistent identity: range2152703
display id: range2152703
version: 1
direction: direction/forward
top level: BBa_K598001
start: 87
end: 806
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152901
display id: annotation2152901
version: 1
top level: BBa_K598001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152901/range2152901/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152901/range2152901/1
type: Range
persistent identity: range2152901
display id: range2152901
version: 1
direction: direction/forward
top level: BBa_K598001
start: 910
end: 929
orientation: inline

Engineered RBS
type: SequenceAnnotation
persistent identity: annotation2152702
display id: annotation2152702
version: 1
top level: BBa_K598001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152702/range2152702/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152702/range2152702/1
type: Range
persistent identity: range2152702
display id: range2152702
version: 1
direction: direction/forward
top level: BBa_K598001
start: 75
end: 81
orientation: inline

Theophylline responsive riboswitch Theo 1G1
type: SequenceAnnotation
persistent identity: annotation2152701
display id: annotation2152701
version: 1
top level: BBa_K598001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152701/range2152701/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152701/range2152701/1
type: Range
persistent identity: range2152701
display id: range2152701
version: 1
direction: direction/forward
top level: BBa_K598001
start: 1
end: 86
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2152882
display id: annotation2152882
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152882/range2152882/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152882/range2152882/1
type: Range
persistent identity: range2152882
display id: range2152882
version: 1
direction: direction/forward
top level: BBa_K598001
start: 815
end: 894
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2152895
display id: annotation2152895
version: 1
top level: BBa_K598001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152895/range2152895/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152895/range2152895/1
type: Range
persistent identity: range2152895
display id: range2152895
version: 1
direction: direction/forward
top level: BBa_K598001
start: 826
end: 869
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2152704
display id: annotation2152704
version: 1
top level: BBa_K598001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152704/range2152704/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152704/range2152704/1
type: Range
persistent identity: range2152704
display id: range2152704
version: 1
direction: direction/forward
top level: BBa_K598001
start: 87
end: 806
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2152705
display id: annotation2152705
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152705/range2152705/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152705/range2152705/1
type: Range
persistent identity: range2152705
display id: range2152705
version: 1
direction: direction/forward
top level: BBa_K598001
start: 903
end: 943
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2152887
display id: annotation2152887
version: 1
top level: BBa_K598001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152887/range2152887/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152887/range2152887/1
type: Range
persistent identity: range2152887
display id: range2152887
version: 1
direction: direction/forward
top level: BBa_K598001
start: 930
end: 943
orientation: inline

BBa_K2040100
type: ComponentDefinition
persistent identity: BBa K2040100
display id: BBa_K2040100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PMcl1, hemolymph inducible promoter from Metarhizium anisopliae
creator: Chilam Poon
created: 2016-10-10T11:00:00Z
modified: 2016-10-16T01:49:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2507_
m user id: 30360
owner id: 30360
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The ubiquitous fungal pathogen Metarhizium anisopliae kills a wide range of insects. Host hemocytes can recognize and ingest its conidia, but this capacity is lost on production of hyphal bodies. The unusual ability of hyphal bodies to avoid detection depends on a gene (Mcl1) that is expressed within 20 min of the pathogen contacting hemolymph. Mcl1 (for Metarhizium collagen-like protein)
mutable notes: NO
mutable provenance: It was isolated from the genomic DNA of our chassis organism Metarhizium anisopliae ARSEF549.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: 5' UTR
sequence: https://synbiohub.org/public/igem/BBa_K2040100_sequence/1

5' UTR
type: SequenceAnnotation
persistent identity: annotation2512760
display id: annotation2512760
version: 1
top level: BBa_K2040100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2040100/annotation2512760/range2512760/1

https://synbiohub.org/public/igem/BBa_K2040100/annotation2512760/range2512760/1
type: Range
persistent identity: range2512760
display id: range2512760
version: 1
direction: direction/forward
top level: BBa_K2040100
start: 2674
end: 2772
orientation: inline

BBa_K1165003
type: ComponentDefinition
persistent identity: BBa K1165003
display id: BBa_K1165003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 2 of XOR Gate 1
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: TAA Bump
sequence annotation: Synthetic Extended Arms
sequence annotation: Target-Binding Arms
sequence annotation: Catalytic Core
sequence annotation: Target Binding Arms
sequence: https://synbiohub.org/public/igem/BBa_K1165003_sequence/1

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2365211
display id: annotation2365211
version: 1
top level: BBa_K1165003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365211/range2365211/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365211/range2365211/1
type: Range
persistent identity: range2365211
display id: range2365211
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 36
end: 38
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365209
display id: annotation2365209
version: 1
top level: BBa_K1165003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365209/range2365209/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365209/range2365209/1
type: Range
persistent identity: range2365209
display id: range2365209
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 1
end: 30
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365210
display id: annotation2365210
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365210/range2365210/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365210/range2365210/1
type: Range
persistent identity: range2365210
display id: range2365210
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 32
end: 35
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365212
display id: annotation2365212
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365212/range2365212/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365212/range2365212/1
type: Range
persistent identity: range2365212
display id: range2365212
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 39
end: 43
orientation: inline

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2365213
display id: annotation2365213
version: 1
top level: BBa_K1165003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365213/range2365213/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365213/range2365213/1
type: Range
persistent identity: range2365213
display id: range2365213
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 44
end: 69
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365215
display id: annotation2365215
version: 1
top level: BBa_K1165003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365215/range2365215/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365215/range2365215/1
type: Range
persistent identity: range2365215
display id: range2365215
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 82
end: 111
orientation: inline

Target Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365214
display id: annotation2365214
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365214/range2365214/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365214/range2365214/1
type: Range
persistent identity: range2365214
display id: range2365214
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 70
end: 81
orientation: inline

BBa_K1486042
type: ComponentDefinition
persistent identity: BBa K1486042
display id: BBa_K1486042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leucine Zipper
creator: EPFL iGem team 2014
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486042_sequence/1

BBa_K1486010
type: ComponentDefinition
persistent identity: BBa K1486010
display id: BBa_K1486010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Cterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486007
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486000
component: BBa_K1486006
component: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486006
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486010_sequence/1

BBa_K1486007
type: Component
persistent identity: component2425006
display id: component2425006
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_B0034
type: Component
persistent identity: component2424997
display id: component2424997
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2425002
display id: component2425002
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
type: Component
persistent identity: component2424991
display id: component2424991
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2425001
display id: component2425001
version: 1
top level: BBa_K1486010
access: public
definition: BBa_K1486000

BBa_K1486006
type: Component
persistent identity: component2424990
display id: component2424990
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_I0500
type: Component
persistent identity: component2424984
display id: component2424984
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2424986
display id: component2424986
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2424995
display id: component2424995
version: 1
top level: BBa_K1486010
access: public
definition: BBa_K1486000

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2425001
display id: annotation2425001
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425001/range2425001/1
component: BBa_K1486000

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type: Range
persistent identity: range2425001
display id: range2425001
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2372
end: 3073
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424986
display id: annotation2424986
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424986/range2424986/1
component: BBa_B0034

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type: Range
persistent identity: range2424986
display id: range2424986
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424991
display id: annotation2424991
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424991/range2424991/1
component: BBa_K1486004

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type: Range
persistent identity: range2424991
display id: range2424991
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1628
end: 1657
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2425002
display id: annotation2425002
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425002/range2425002/1
component: BBa_K1486004

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type: Range
persistent identity: range2425002
display id: range2425002
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3074
end: 3103
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2425006
display id: annotation2425006
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
type: Range
persistent identity: range2425006
display id: range2425006
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3104
end: 3688
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424995
display id: annotation2424995
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424995/range2424995/1
component: BBa_K1486000

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type: Range
persistent identity: range2424995
display id: range2424995
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1658
end: 2359
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424997
display id: annotation2424997
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424997/range2424997/1
component: BBa_B0034

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type: Range
persistent identity: range2424997
display id: range2424997
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2360
end: 2371
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424990
display id: annotation2424990
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424990/range2424990/1
component: BBa_K1486006

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type: Range
persistent identity: range2424990
display id: range2424990
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1223
end: 1627
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424984
display id: annotation2424984
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424984/range2424984/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424984/range2424984/1
type: Range
persistent identity: range2424984
display id: range2424984
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1
end: 1210
orientation: inline

BBa_K772005
type: ComponentDefinition
persistent identity: BBa K772005
display id: BBa_K772005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL generator: LasI
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated. This part represents an alternative quorum sensing sender device which is designed to induce PoPS production in the parts including pLas such as BBa_K772004. LasI is an enzyme generator gene sequences which synthesizes 6HSLO3 (AHL) in order to induce a PoPS response in the pLas promoter. pLas promoter works in the presence of both 6HSLO3 and LasR protein.
mutable notes: Note that there is an RBS sequence just before LasI; in order to be easily used with quorum sensing receiver devices such as BBa_F2620 or BBa_K772004.
mutable provenance: LasI sequence is gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: LacI
sequence annotation: pLac
sequence annotation: Terminator
sequence annotation: RBS
sequence annotation: LasI
sequence: https://synbiohub.org/public/igem/BBa_K772005_sequence/1

LacI
type: SequenceAnnotation
persistent identity: annotation2203712
display id: annotation2203712
version: 1
top level: BBa_K772005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203712/range2203712/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203712/range2203712/1
type: Range
persistent identity: range2203712
display id: range2203712
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1
end: 1132
orientation: inline

pLac
type: SequenceAnnotation
persistent identity: annotation2203713
display id: annotation2203713
version: 1
top level: BBa_K772005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203713/range2203713/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203713/range2203713/1
type: Range
persistent identity: range2203713
display id: range2203713
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1133
end: 1331
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2203715
display id: annotation2203715
version: 1
top level: BBa_K772005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203715/range2203715/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203715/range2203715/1
type: Range
persistent identity: range2203715
display id: range2203715
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1969
end: 2098
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203714
display id: annotation2203714
version: 1
top level: BBa_K772005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203714/range2203714/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203714/range2203714/1
type: Range
persistent identity: range2203714
display id: range2203714
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1332
end: 1352
orientation: inline

LasI
type: SequenceAnnotation
persistent identity: annotation2329745
display id: annotation2329745
version: 1
top level: BBa_K772005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2329745/range2329745/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2329745/range2329745/1
type: Range
persistent identity: range2329745
display id: range2329745
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1353
end: 1968
orientation: inline

TFBS
type: ComponentDefinition
persistent identity: BBa K2127006
display id: BBa_K2127006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transcription Factor Binding Site from Cyanobacterial Pcpc560
creator: Mirat Sojitra
created: 2016-10-13T11:00:00Z
modified: 2016-10-25T10:25:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2596_
m user id: 32415
owner id: 32736
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Pcpc560-TFBS
mutable notes: Pcpc560-TFBS
mutable provenance: Pcpc560-TFBS
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Transcription Factor Binding Sites
sequence: https://synbiohub.org/public/igem/BBa_K2127006_sequence/1

Transcription Factor Binding Sites
type: SequenceAnnotation
persistent identity: annotation2530242
display id: annotation2530242
version: 1
top level: TFBS
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2127006/annotation2530242/range2530242/1

https://synbiohub.org/public/igem/BBa_K2127006/annotation2530242/range2530242/1
type: Range
persistent identity: range2530242
display id: range2530242
version: 1
direction: direction/forward
top level: TFBS
start: 1
end: 185
orientation: inline

Fur
type: ComponentDefinition
persistent identity: BBa K1122666
display id: BBa_K1122666
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ferric uptake repressor
creator: Hugo Villanueva
created: 2013-07-23T11:00:00Z
modified: 2015-07-24T01:14:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 4206
owner id: 18022
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The ferric uptake repressor of Bacillus subtilis called YqkL is a regulator that when in contact with iron will bind and repress a regulatory sequence (the Fur box).
mutable notes: We sequenced the Fur box. This can be added in front of genes to provide regulation when in contact with iron.
mutable provenance: This comes from Bacillus subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122666_sequence/1

BBa_K1486000
type: ComponentDefinition
persistent identity: BBa K1486000
display id: BBa_K1486000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR coding region
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CpxR is a transciption factor that dimerizes and becomes active when phosphorylated by the transmembrane histidine kinase CpxA. CpxA in turn becomes active when the bacterial cell is submitted to different kinds of shock (pH, temperature, pressure, protein misfolding in the inter-membrane space...).
mutable notes: When making composite parts with CpxR that are supposed to dimerize, we had to take care about the positioning of the other elements regarding the parallel dimerization of CpxR.
mutable provenance: The part has been extracted from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CpxR
sequence annotation: STOP
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_K1486000_sequence/1

CpxR
type: SequenceAnnotation
persistent identity: annotation2382021
display id: annotation2382021
version: 1
top level: BBa_K1486000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382021/range2382021/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382021/range2382021/1
type: Range
persistent identity: range2382021
display id: range2382021
version: 1
direction: direction/forward
top level: BBa_K1486000
start: 1
end: 702
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2382023
display id: annotation2382023
version: 1
top level: BBa_K1486000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382023/range2382023/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382023/range2382023/1
type: Range
persistent identity: range2382023
display id: range2382023
version: 1
direction: direction/forward
top level: BBa_K1486000
start: 699
end: 702
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2382022
display id: annotation2382022
version: 1
top level: BBa_K1486000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382022/range2382022/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382022/range2382022/1
type: Range
persistent identity: range2382022
display id: range2382022
version: 1
direction: direction/forward
top level: BBa_K1486000
start: 1
end: 3
orientation: inline

BBa_K1486040
type: ComponentDefinition
persistent identity: BBa K1486040
display id: BBa_K1486040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPN + CpxR
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486000
component: BBa_K1486038
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486037
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486038
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486040_sequence/1

BBa_K1486000
type: Component
persistent identity: component2387193
display id: component2387193
version: 1
top level: BBa_K1486040
access: public
definition: BBa_K1486000

BBa_K1486038
type: Component
persistent identity: component2387188
display id: component2387188
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486038/1

BBa_B0034
type: Component
persistent identity: component2387187
display id: component2387187
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2387185
display id: component2387185
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486037
type: Component
persistent identity: component2387189
display id: component2387189
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387189
display id: annotation2387189
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387189/range2387189/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387189/range2387189/1
type: Range
persistent identity: range2387189
display id: range2387189
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1865
end: 1903
orientation: inline

BBa_K1486038
type: SequenceAnnotation
persistent identity: annotation2387188
display id: annotation2387188
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
component: BBa_K1486038

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
type: Range
persistent identity: range2387188
display id: range2387188
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1223
end: 1864
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387187
display id: annotation2387187
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387187/range2387187/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387187/range2387187/1
type: Range
persistent identity: range2387187
display id: range2387187
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387193
display id: annotation2387193
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387193/range2387193/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387193/range2387193/1
type: Range
persistent identity: range2387193
display id: range2387193
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1904
end: 2605
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387185
display id: annotation2387185
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
type: Range
persistent identity: range2387185
display id: range2387185
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1
end: 1210
orientation: inline

BBa_K1486015
type: ComponentDefinition
persistent identity: BBa K1486015
display id: BBa_K1486015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[2]-CpxR under pBAD
creator: EPFL iGem team 2014
created: 2014-09-09T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[2]-CpxR. Biobrick BBa_K1486014 has IFP[1]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486013 is CpxR-IFP[2] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To edit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_K1486007
component: BBa_B0034
component: BBa_K1486000
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486015_sequence/1

BBa_K1486004
type: Component
persistent identity: component2382855
display id: component2382855
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486007
type: Component
persistent identity: component2382854
display id: component2382854
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_B0034
type: Component
persistent identity: component2382850
display id: component2382850
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382859
display id: component2382859
version: 1
top level: BBa_K1486015
access: public
definition: BBa_K1486000

BBa_I0500
type: Component
persistent identity: component2382848
display id: component2382848
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382848
display id: annotation2382848
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
type: Range
persistent identity: range2382848
display id: range2382848
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382850
display id: annotation2382850
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
type: Range
persistent identity: range2382850
display id: range2382850
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1219
end: 1230
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382859
display id: annotation2382859
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
type: Range
persistent identity: range2382859
display id: range2382859
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1860
end: 2561
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382855
display id: annotation2382855
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
type: Range
persistent identity: range2382855
display id: range2382855
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1824
end: 1853
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382854
display id: annotation2382854
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
type: Range
persistent identity: range2382854
display id: range2382854
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1237
end: 1815
orientation: inline

BBa_K1486012
type: ComponentDefinition
persistent identity: BBa K1486012
display id: BBa_K1486012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[1] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[1]. Biobrick BBa_K1486013 has CpxR-IFP[2] with an arabinose promoter. This construct was made in both orientations: BBa_K1486014 is IFP[1]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486000
component: BBa_K1486006
component: BBa_K1486004
component: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence: https://synbiohub.org/public/igem/BBa_K1486012_sequence/1

BBa_B0034
type: Component
persistent identity: component2382287
display id: component2382287
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382291
display id: component2382291
version: 1
top level: BBa_K1486012
access: public
definition: BBa_K1486000

BBa_K1486006
type: Component
persistent identity: component2382293
display id: component2382293
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486004
type: Component
persistent identity: component2382292
display id: component2382292
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_I0500
type: Component
persistent identity: component2382285
display id: component2382285
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382287
display id: annotation2382287
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
type: Range
persistent identity: range2382287
display id: range2382287
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382285
display id: annotation2382285
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
type: Range
persistent identity: range2382285
display id: range2382285
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1
end: 1210
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382293
display id: annotation2382293
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
type: Range
persistent identity: range2382293
display id: range2382293
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1955
end: 2536
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382291
display id: annotation2382291
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
type: Range
persistent identity: range2382291
display id: range2382291
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1223
end: 1924
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382292
display id: annotation2382292
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
type: Range
persistent identity: range2382292
display id: range2382292
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1925
end: 1954
orientation: inline

BBa_K1486049
type: ComponentDefinition
persistent identity: BBa K1486049
display id: BBa_K1486049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter FW + RFP
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_K1486046
sequence annotation: BBa_I13507
sequence annotation: BBa_K1486046
sequence: https://synbiohub.org/public/igem/BBa_K1486049_sequence/1

BBa_I13507
type: Component
persistent identity: component2398436
display id: component2398436
version: 1
top level: BBa_K1486049
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K1486046
type: Component
persistent identity: component2398424
display id: component2398424
version: 1
top level: BBa_K1486049
access: public
definition: BBa_K1486046

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398436
display id: annotation2398436
version: 1
top level: BBa_K1486049
location: https://synbiohub.org/public/igem/BBa_K1486049/annotation2398436/range2398436/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K1486049/annotation2398436/range2398436/1
type: Range
persistent identity: range2398436
display id: range2398436
version: 1
direction: direction/reverse
top level: BBa_K1486049
start: 155
end: 1015
orientation: inline

BBa_K1486046
type: SequenceAnnotation
persistent identity: annotation2398424
display id: annotation2398424
version: 1
top level: BBa_K1486049
location: https://synbiohub.org/public/igem/BBa_K1486049/annotation2398424/range2398424/1
component: BBa_K1486046

https://synbiohub.org/public/igem/BBa_K1486049/annotation2398424/range2398424/1
type: Range
persistent identity: range2398424
display id: range2398424
version: 1
direction: direction/reverse
top level: BBa_K1486049
start: 1
end: 146
orientation: inline

BBa_K1486008
type: ComponentDefinition
persistent identity: BBa K1486008
display id: BBa_K1486008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][1]
creator: iGem EPFL 2014
created: 2014-08-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - IFP[1]-CpxR + IFP[2]-CpxR (BBa_K1286009) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486052
component: BBa_K1486000
component: BBa_K1486004
component: BBa_B0034
component: BBa_K1486007
component: BBa_K1486006
component: BBa_I0500
sequence annotation: BBa_K1486052
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486006
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486008_sequence/1

BBa_K1486052
type: Component
persistent identity: component2418046
display id: component2418046
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_K1486000
type: Component
persistent identity: component2418040
display id: component2418040
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486000

BBa_K1486004
type: Component
persistent identity: component2418041
display id: component2418041
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2418052
display id: component2418052
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486000

BBa_K1486004
type: Component
persistent identity: component2418053
display id: component2418053
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2418036
display id: component2418036
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2418048
display id: component2418048
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486007
type: Component
persistent identity: component2418057
display id: component2418057
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486006
type: Component
persistent identity: component2418045
display id: component2418045
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_I0500
type: Component
persistent identity: component2418034
display id: component2418034
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2418046
display id: annotation2418046
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
component: BBa_K1486052

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
type: Range
persistent identity: range2418046
display id: range2418046
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2360
end: 2397
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418040
display id: annotation2418040
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
type: Range
persistent identity: range2418040
display id: range2418040
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1223
end: 1924
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2418045
display id: annotation2418045
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418045/range2418045/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418045/range2418045/1
type: Range
persistent identity: range2418045
display id: range2418045
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1955
end: 2359
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418052
display id: annotation2418052
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
type: Range
persistent identity: range2418052
display id: range2418052
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2410
end: 3111
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418048
display id: annotation2418048
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
type: Range
persistent identity: range2418048
display id: range2418048
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2398
end: 2409
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418036
display id: annotation2418036
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418036/range2418036/1
component: BBa_B0034

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type: Range
persistent identity: range2418036
display id: range2418036
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418041
display id: annotation2418041
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418041/range2418041/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418041/range2418041/1
type: Range
persistent identity: range2418041
display id: range2418041
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1925
end: 1954
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418053
display id: annotation2418053
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418053/range2418053/1
component: BBa_K1486004

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type: Range
persistent identity: range2418053
display id: range2418053
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3112
end: 3141
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2418034
display id: annotation2418034
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
type: Range
persistent identity: range2418034
display id: range2418034
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1
end: 1210
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2418057
display id: annotation2418057
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418057/range2418057/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418057/range2418057/1
type: Range
persistent identity: range2418057
display id: range2418057
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3142
end: 3726
orientation: inline

BBa_K1165004
type: ComponentDefinition
persistent identity: BBa K1165004
display id: BBa_K1165004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 1 of XOR Gate 2
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: TAA Bump
sequence annotation: Target-Binding Arms
sequence annotation: Synthetic Extended Arms
sequence annotation: Target Binding Arms
sequence annotation: Catalytic Core
sequence: https://synbiohub.org/public/igem/BBa_K1165004_sequence/1

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2365273
display id: annotation2365273
version: 1
top level: BBa_K1165004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365273/range2365273/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365273/range2365273/1
type: Range
persistent identity: range2365273
display id: range2365273
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 36
end: 38
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365279
display id: annotation2365279
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365279/range2365279/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365279/range2365279/1
type: Range
persistent identity: range2365279
display id: range2365279
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 70
end: 81
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365271
display id: annotation2365271
version: 1
top level: BBa_K1165004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365271/range2365271/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365271/range2365271/1
type: Range
persistent identity: range2365271
display id: range2365271
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 1
end: 30
orientation: inline

Target Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365277
display id: annotation2365277
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
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type: Range
persistent identity: range2365277
display id: range2365277
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 39
end: 43
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365272
display id: annotation2365272
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365272/range2365272/1

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type: Range
persistent identity: range2365272
display id: range2365272
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 33
end: 35
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365280
display id: annotation2365280
version: 1
top level: BBa_K1165004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365280/range2365280/1

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type: Range
persistent identity: range2365280
display id: range2365280
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 82
end: 111
orientation: inline

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2365278
display id: annotation2365278
version: 1
top level: BBa_K1165004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365278/range2365278/1

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type: Range
persistent identity: range2365278
display id: range2365278
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 44
end: 69
orientation: inline

BBa_K1486009
type: ComponentDefinition
persistent identity: BBa K1486009
display id: BBa_K1486009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_K1486000
component: BBa_I0500
component: BBa_K1486006
component: BBa_K1486007
component: BBa_B0034
component: BBa_K1486052
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486006
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486052
sequence: https://synbiohub.org/public/igem/BBa_K1486009_sequence/1

BBa_K1486004
type: Component
persistent identity: component2420772
display id: component2420772
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2420788
display id: component2420788
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_K1486004
type: Component
persistent identity: component2420784
display id: component2420784
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_I0500
type: Component
persistent identity: component2420763
display id: component2420763
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2420776
display id: component2420776
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_K1486006
type: Component
persistent identity: component2420771
display id: component2420771
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486004
type: Component
persistent identity: component2420758
display id: component2420758
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486007
type: Component
persistent identity: component2420783
display id: component2420783
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_B0034
type: Component
persistent identity: component2420767
display id: component2420767
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2420766
display id: component2420766
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2420779
display id: component2420779
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486007
type: Component
persistent identity: component2420756
display id: component2420756
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486052
type: Component
persistent identity: component2420777
display id: component2420777
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420767
display id: annotation2420767
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420767/range2420767/1
component: BBa_B0034

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persistent identity: range2420767
display id: range2420767
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1211
end: 1222
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2420771
display id: annotation2420771
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420771/range2420771/1
component: BBa_K1486006

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type: Range
persistent identity: range2420771
display id: range2420771
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1223
end: 1627
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2420763
display id: annotation2420763
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420763/range2420763/1
component: BBa_I0500

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type: Range
persistent identity: range2420763
display id: range2420763
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1
end: 1210
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420758
display id: annotation2420758
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420758/range2420758/1
component: BBa_K1486004

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persistent identity: range2420758
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direction: direction/reverse
top level: BBa_K1486009
start: 2995
end: 3024
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420783
display id: annotation2420783
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420783/range2420783/1
component: BBa_K1486007

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type: Range
persistent identity: range2420783
display id: range2420783
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2410
end: 2994
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420756
display id: annotation2420756
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420756/range2420756/1
component: BBa_K1486007

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type: Range
persistent identity: range2420756
display id: range2420756
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2410
end: 2994
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420772
display id: annotation2420772
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420772/range2420772/1
component: BBa_K1486004

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type: Range
persistent identity: range2420772
display id: range2420772
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1628
end: 1657
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420779
display id: annotation2420779
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420779/range2420779/1
component: BBa_B0034

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type: Range
persistent identity: range2420779
display id: range2420779
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2398
end: 2409
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420766
display id: annotation2420766
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420766/range2420766/1
component: BBa_K1486000

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type: Range
persistent identity: range2420766
display id: range2420766
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420776
display id: annotation2420776
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420776/range2420776/1
component: BBa_K1486000

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type: Range
persistent identity: range2420776
display id: range2420776
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1658
end: 2359
orientation: inline

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2420777
display id: annotation2420777
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420777/range2420777/1
component: BBa_K1486052

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type: Range
persistent identity: range2420777
display id: range2420777
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2360
end: 2397
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420784
display id: annotation2420784
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420784/range2420784/1
component: BBa_K1486004

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type: Range
persistent identity: range2420784
display id: range2420784
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2995
end: 3024
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420788
display id: annotation2420788
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420788/range2420788/1
component: BBa_K1486000

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type: Range
persistent identity: range2420788
display id: range2420788
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
orientation: inline

BBa_K1486046
type: ComponentDefinition
persistent identity: BBa K1486046
display id: BBa_K1486046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter FW
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21033
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Test
mutable notes: Test
mutable provenance: Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1486046_sequence/1

BBa_K1165002
type: ComponentDefinition
persistent identity: BBa K1165002
display id: BBa_K1165002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 1 of XOR Gate 1
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the DNA sequence of a Y-type hammerhead ribozyme that targets and cleaves the PenI repressor (Part BBa_C0074). This ribozyme has target-binding flanking arms adjacent to the Y-type hammerhead catalytic core and synthetic extended arms located towards the 5' and 3' ends. The catalytic core allows for the sequence-specific cleavage of sites within the coding sequence of PenI. Hammerhead ribozymes are able to cleave at any NUH within an RNA sequence, where H represent any nucleotide except guanine (Mir 2001), although with GUC as the hammerhead consensus cleavage site (Lieber, 1995). The binding of the flanking arms adjacent to the hammerhead catalytic core favours the recognition and cleavage of only one target site. Additional secondary structure within the flanking arms was detected to a small degree by using IDT OligoAnalyzer's Hairpin tool (Owczarzy 2008) as is depicted below. [image] The synthetic extended arms are designed to bind to the complementary extended arms of another similar PenI-cleaving ribozyme (Part _____), but do not contribute to any additional secondary structure as confirmed via the Hairpin tool in IDT's OligoAnalyzer (Owczarzy 2008), as both arms of a particular ribozyme only contain a purine and pyrimidine pair that do not bind to each other (in this case, A and G). The two-nucleotide extended arms are also staggered in number to avoid slippage in binding and bind to its complement pair at significantly higher melting temperatures than the adjacent flanking arms. This feature allows for the sequestering of the two ribozymes when both are present and significantly decreasing any ribozyme activity. Regions of complementarity between the two target-binding regions of both ribozymes are also present to ensure that the functional catalytic cores are not able to cleave their mRNA targets, even when bound. The characteristics of this expressed pair of ribozymes were considered to create a functional RNA-based XOR logic gate. References Mir, A…
mutable notes: Although this particular part is likely to only cleave the PenI repressor mRNA due to its sequence specificity, the simple in silico design of this ribozyme allows for the cleavage of virtually any RNA sequence containing a cleave site (NUH). This particular sequence was obtained by first scanning our target PenI mRNA for the optimal cut site GUC. The 10 nucleotides surrounding this cut site were obtained and the complement of this area was generated for the ribozyme flanking arms. The length of this region was decided by ideal melting temperature (51.4 degrees Celsius). Additional synthetic extended arms were subsequently added to the 5' and 3' ends of the sequence for binding to the complement ribozyme in an XOR gate. This region of non-pairing purines and pyrimidines was staggered in base repeats to avoid slippage of binding and was defined in length by significantly higher melting temperatures (approximately 68 degrees Celsius). Finally, the catalytic core of the Y-type hammerhead ribozyme was introduced at the appropriate region. Throughout this entire process, secondary was continually checked and minimized using IDT OligoAnalyzer's Hairpin tool (Owczarzy 2008). The complete ribozyme sequences was also BLASTed again the E.coli genome, which was our chassis and yielded insignificant results. Other factors such as differential length or target binding regions due to varying melting temperature and GC content were also considered during the in silico design of this ribozyme and its pair in the XOR logic gate. References Owczarzy, R. et al. IDT SciTools: a suite for analysis and design of nucleic acid oligomers. Nucleic Acids Res 36, W163???W169 (2008).
mutable provenance: The target-binding flanking arms of the ribozyme were obtained from the complement of the PenI Repressor sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Target-Binding Arms
sequence annotation: Synthetic Extended Arms
sequence annotation: Catalytic Core
sequence annotation: TAA Bump
sequence: https://synbiohub.org/public/igem/BBa_K1165002_sequence/1

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364288
display id: annotation2364288
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364288/range2364288/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364288/range2364288/1
type: Range
persistent identity: range2364288
display id: range2364288
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 70
end: 81
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364284
display id: annotation2364284
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364284/range2364284/1

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type: Range
persistent identity: range2364284
display id: range2364284
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 31
end: 35
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2364282
display id: annotation2364282
version: 1
top level: BBa_K1165002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364282/range2364282/1

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type: Range
persistent identity: range2364282
display id: range2364282
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 1
end: 30
orientation: inline

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2364287
display id: annotation2364287
version: 1
top level: BBa_K1165002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364287/range2364287/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364287/range2364287/1
type: Range
persistent identity: range2364287
display id: range2364287
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 44
end: 69
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364285
display id: annotation2364285
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364285/range2364285/1

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type: Range
persistent identity: range2364285
display id: range2364285
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 39
end: 43
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2364283
display id: annotation2364283
version: 1
top level: BBa_K1165002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364283/range2364283/1

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type: Range
persistent identity: range2364283
display id: range2364283
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 82
end: 111
orientation: inline

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2364286
display id: annotation2364286
version: 1
top level: BBa_K1165002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364286/range2364286/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364286/range2364286/1
type: Range
persistent identity: range2364286
display id: range2364286
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 36
end: 38
orientation: inline

BBa_K1486041
type: ComponentDefinition
persistent identity: BBa K1486041
display id: BBa_K1486041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPC + CpxR
creator: Ione Pla Puigsubir??
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486039
component: BBa_K1486037
component: BBa_B0034
component: BBa_K1486000
component: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486039
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486041_sequence/1

BBa_K1486039
type: Component
persistent identity: component2387212
display id: component2387212
version: 1
top level: BBa_K1486041
access: public
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persistent identity: component2387213
display id: component2387213
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_B0034
type: Component
persistent identity: component2387211
display id: component2387211
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2387217
display id: component2387217
version: 1
top level: BBa_K1486041
access: public
definition: BBa_K1486000

BBa_I0500
type: Component
persistent identity: component2387209
display id: component2387209
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387217
display id: annotation2387217
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
component: BBa_K1486000

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type: Range
persistent identity: range2387217
display id: range2387217
version: 1
direction: direction/reverse
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start: 1334
end: 2035
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387211
display id: annotation2387211
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
component: BBa_B0034

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type: Range
persistent identity: range2387211
display id: range2387211
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1211
end: 1222
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387213
display id: annotation2387213
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
component: BBa_K1486037

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type: Range
persistent identity: range2387213
display id: range2387213
version: 1
direction: direction/reverse
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start: 1295
end: 1333
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BBa_K1486039
type: SequenceAnnotation
persistent identity: annotation2387212
display id: annotation2387212
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
component: BBa_K1486039

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type: Range
persistent identity: range2387212
display id: range2387212
version: 1
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start: 1223
end: 1294
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BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387209
display id: annotation2387209
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
component: BBa_I0500

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type: Range
persistent identity: range2387209
display id: range2387209
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1
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BBa_K1486005
type: ComponentDefinition
persistent identity: BBa K1486005
display id: BBa_K1486005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP-CpxR [Cterm]
creator: iGem EPFL 2014
created: 2014-08-12T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_I746916
component: BBa_K1486000
component: BBa_K1486003
component: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_I746916
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486003
sequence: https://synbiohub.org/public/igem/BBa_K1486005_sequence/1

BBa_I0500
type: Component
persistent identity: component2382082
display id: component2382082
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I746916
type: Component
persistent identity: component2382093
display id: component2382093
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_K1486000
type: Component
persistent identity: component2382088
display id: component2382088
version: 1
top level: BBa_K1486005
access: public
definition: BBa_K1486000

BBa_K1486003
type: Component
persistent identity: component2382089
display id: component2382089
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_B0034
type: Component
persistent identity: component2382084
display id: component2382084
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382082
display id: annotation2382082
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
component: BBa_I0500

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type: Range
persistent identity: range2382082
display id: range2382082
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1
end: 1210
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382093
display id: annotation2382093
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
component: BBa_I746916

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type: Range
persistent identity: range2382093
display id: range2382093
version: 1
direction: direction/reverse
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start: 1949
end: 2668
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382088
display id: annotation2382088
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
component: BBa_K1486000

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type: Range
persistent identity: range2382088
display id: range2382088
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382084
display id: annotation2382084
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
component: BBa_B0034

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type: Range
persistent identity: range2382084
display id: range2382084
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1211
end: 1222
orientation: inline

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382089
display id: annotation2382089
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382089/range2382089/1
component: BBa_K1486003

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type: Range
persistent identity: range2382089
display id: range2382089
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1925
end: 1948
orientation: inline

BBa_K259007
type: ComponentDefinition
persistent identity: BBa K259007
display id: BBa_K259007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Promoter fused with RBS
creator: Petros Mina
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has the arabinose regulated promoter (AraC/pBAD) upstream of a ribosome binding site. This is ideal for inserting a protein to be expressed downstream of this part as it's expression can be regulated by the presence of the concentration of arabinose in the growing environment/media of the cells. ==What this part actually does== This composite part includes the AraC/pBAD promoter and a strong ribosome binding site designed from the Anderson family of RBS. The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the trancription of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. Controlling the transcription will not ensure translation. The ribosome binding site is ideally placed a small distance away from the promoter site to ensure that the ribosome will bind and start translating the protein coding sequence found downstream.
mutable notes: The part was designed with RFC10 assembly from supplied biobricks of the iGEM2009 distribution.
mutable provenance: The AraC promoter can be found naturally at 1.52min of the E.coli genome ([http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene number EG10054]. The RBS was taken from the designed sequences of RBS by the Anderson family. For more information visit the original part page [http://partsregistry.org/Part:BBa_J61100 here].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J61100
component: BBa_R0080
component: BBa_R0081
sequence annotation: BBa_R0081
sequence annotation: BBa_R0080
sequence annotation: BBa_J61100
sequence: https://synbiohub.org/public/igem/BBa_K259007_sequence/1

BBa_J61100
type: Component
persistent identity: component2020547
display id: component2020547
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_R0080
type: Component
persistent identity: component2020546
display id: component2020546
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_R0081
type: Component
persistent identity: component2020539
display id: component2020539
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2020539
display id: annotation2020539
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
component: BBa_R0081

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type: Range
persistent identity: range2020539
display id: range2020539
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 1
end: 183
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2020546
display id: annotation2020546
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
component: BBa_R0080

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type: Range
persistent identity: range2020546
display id: range2020546
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 192
end: 340
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2020547
display id: annotation2020547
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
component: BBa_J61100

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type: Range
persistent identity: range2020547
display id: range2020547
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 349
end: 360
orientation: inline

BBa_K1824012
type: ComponentDefinition
persistent identity: BBa K1824012
display id: BBa_K1824012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + TetO + RBS
creator: Zixu Wang
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T10:05:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Modified constitutive promoter, which can be repressed by TetR and induce by tetracycline or aTc.
mutable notes: no special concern
mutable provenance: Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. B0030 is a strong RBS based on Ron Weiss thesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1824890
component: BBa_K1824889
component: BBa_J64991
component: BBa_J23100
component: BBa_B0030
sequence annotation: BBa_K1824890
sequence annotation: BBa_B0030
sequence annotation: BBa_J64991
sequence annotation: BBa_J23100
sequence annotation: BBa_K1824889
sequence: https://synbiohub.org/public/igem/BBa_K1824012_sequence/1

BBa_K1824890
type: Component
persistent identity: component2452354
display id: component2452354
version: 1
top level: BBa_K1824012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824890/1

BBa_K1824889
type: Component
persistent identity: component2452350
display id: component2452350
version: 1
top level: BBa_K1824012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_J64991
type: Component
persistent identity: component2452349
display id: component2452349
version: 1
top level: BBa_K1824012
access: public
definition: https://synbiohub.org/public/igem/BBa_J64991/1

BBa_J23100
type: Component
persistent identity: component2452348
display id: component2452348
version: 1
top level: BBa_K1824012
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0030
type: Component
persistent identity: component2452352
display id: component2452352
version: 1
top level: BBa_K1824012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2452354
display id: annotation2452354
version: 1
top level: BBa_K1824012
location: https://synbiohub.org/public/igem/BBa_K1824012/annotation2452354/range2452354/1
component: BBa_K1824890

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type: Range
persistent identity: range2452354
display id: range2452354
version: 1
direction: direction/reverse
top level: BBa_K1824012
start: 78
end: 83
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2452352
display id: annotation2452352
version: 1
top level: BBa_K1824012
location: https://synbiohub.org/public/igem/BBa_K1824012/annotation2452352/range2452352/1
component: BBa_B0030

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type: Range
persistent identity: range2452352
display id: range2452352
version: 1
direction: direction/forward
top level: BBa_K1824012
start: 63
end: 77
orientation: inline

BBa_J64991
type: SequenceAnnotation
persistent identity: annotation2452349
display id: annotation2452349
version: 1
top level: BBa_K1824012
location: https://synbiohub.org/public/igem/BBa_K1824012/annotation2452349/range2452349/1
component: BBa_J64991

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type: Range
persistent identity: range2452349
display id: range2452349
version: 1
direction: direction/reverse
top level: BBa_K1824012
start: 36
end: 54
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2452348
display id: annotation2452348
version: 1
top level: BBa_K1824012
location: https://synbiohub.org/public/igem/BBa_K1824012/annotation2452348/range2452348/1
component: BBa_J23100

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type: Range
persistent identity: range2452348
display id: range2452348
version: 1
direction: direction/reverse
top level: BBa_K1824012
start: 1
end: 35
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2452350
display id: annotation2452350
version: 1
top level: BBa_K1824012
location: https://synbiohub.org/public/igem/BBa_K1824012/annotation2452350/range2452350/1
component: BBa_K1824889

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type: Range
persistent identity: range2452350
display id: range2452350
version: 1
direction: direction/reverse
top level: BBa_K1824012
start: 55
end: 62
orientation: inline

BBa_K1315003
type: ComponentDefinition
persistent identity: BBa K1315003
display id: BBa_K1315003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The rpfG gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the coding sequence for the RpfG(regulation of pathogenicity factor G) protein. It is the regulator in the two-component signal transduction system of Xanthomonas campestris pv. campestris that is activated by the sensor kinase RpfC(Bba_K1315002) in the presence of DSF(diffusible signal factor).
mutable notes: The sequence had 1 EcoRI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris(Xcc) genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RpfG
sequence: https://synbiohub.org/public/igem/BBa_K1315003_sequence/1

RpfG
type: SequenceAnnotation
persistent identity: annotation2386405
display id: annotation2386405
version: 1
top level: BBa_K1315003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315003/annotation2386405/range2386405/1

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type: Range
persistent identity: range2386405
display id: range2386405
version: 1
direction: direction/forward
top level: BBa_K1315003
start: 1
end: 1140
orientation: inline

BBa_K1486056
type: ComponentDefinition
persistent identity: BBa K1486056
display id: BBa_K1486056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][2]
creator: iGem EPFL 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the twin of the non-compatible biobrick BBa_K1486008 rendered compatible by mutating the two illegal restriction sites. However, all experiments were run on the aforementioned biobrick.
mutable notes: Site-directed mutagenesis was used to remove the restriction sites in the sequence.
mutable provenance: http://www.addgene.org/browse/article/8177/ which was then mutated by us.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1486004
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486000
component: BBa_K1486045
component: BBa_K1486044
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486045
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486044
sequence: https://synbiohub.org/public/igem/BBa_K1486056_sequence/1

BBa_K1486004
type: Component
persistent identity: component2398606
display id: component2398606
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2398609
display id: component2398609
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2398599
display id: component2398599
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2398605
display id: component2398605
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2398601
display id: component2398601
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486045
type: Component
persistent identity: component2398615
display id: component2398615
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486045/1

BBa_K1486004
type: Component
persistent identity: component2398614
display id: component2398614
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2398613
display id: component2398613
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486000

BBa_K1486044
type: Component
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BBa_B0034
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BBa_K1486045
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BBa_K1486000
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BBa_I0500
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BBa_K1486004
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BBa_K1486044
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persistent identity: annotation2398607
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BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2398613
display id: annotation2398613
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BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2398614
display id: annotation2398614
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398601
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BBa_K1315007
type: ComponentDefinition
persistent identity: BBa K1315007
display id: BBa_K1315007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The BCAM0228 Response Regulator from Burkholderia
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the coding sequence of the BCAM0228 two-component regulator of the BDSF sensory BCAM0227-BCAM0228 system. This system is found in Burkholderia spp.
mutable notes: The sequence had 1 EcoRI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Burkholderia cenocepacia J2315 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BCAM0228
sequence: https://synbiohub.org/public/igem/BBa_K1315007_sequence/1

BCAM0228
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persistent identity: annotation2386928
display id: annotation2386928
version: 1
top level: BBa_K1315007
role: feature/cds
role: SO:0000316
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BBa_K1486050
type: ComponentDefinition
persistent identity: BBa K1486050
display id: BBa_K1486050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter RV + RFP
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1486047
component: BBa_I13507
sequence annotation: BBa_I13507
sequence annotation: BBa_K1486047
sequence: https://synbiohub.org/public/igem/BBa_K1486050_sequence/1

BBa_K1486047
type: Component
persistent identity: component2398450
display id: component2398450
version: 1
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access: public
definition: BBa_K1486047

BBa_I13507
type: Component
persistent identity: component2398462
display id: component2398462
version: 1
top level: BBa_K1486050
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398462
display id: annotation2398462
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BBa_K1486047
type: SequenceAnnotation
persistent identity: annotation2398450
display id: annotation2398450
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creB
type: ComponentDefinition
persistent identity: BBa J64969
display id: BBa_J64969
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CreB Transcriptional Regulator
creator: Kristopher Kuchenbecker
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: regulator
mutable notes: regulator
mutable provenance: regulator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J64969_sequence/1