Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
PT7 + RBS + SbpA || Red || BisdA || BisdB
S-Layer SbpA from Lysinibacillus sphaericus with T7/lac
S-Layer SgsE from Geobacillus stearothermophilus with T7/lac
This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.
A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an A, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
T7 promoter with an A->T mutation at the -16 position of the consensus sequence
Fusion Protein of S-Layer SgsE and LigA with His-Tag
SgsE was synthesized, LigA with His-Tag created by PCR. LigA is the natural ligase A of E. coli.
This part was assembled by Freiburg Standard Assembly (RFC 25)
The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
Two T7 promoters are reversely overlapping in five base pairs, with the third one mutated from A to G.
It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
S-layer cspB from Corynebacterium halotolerans with TAT-Sequence and lipid anchor, PT7 and RBS
pT7 + RBS + S-Layer + TAT-Sequenz + Lipidanker
T7 promoter with an A->T mutation at the -10 position of the consensus sequence
T7 promoter for expression of functional RNA
to be used qith t7 polymerase BBa_J34811
S-Layer SgsE from Geobacillus stearothermophilus
S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS
S-layer cspB from Corynebacterium halotolerans with PT7 and RBS
The T3 promoter comes from the T3 bacteriophage. The RBS site comes from...
This is the T3 promoter with an RBS site added. Other groups should use this part to regulate their part by adding it in front.
S-layer cspB from Corynebacterium glutamicum with lipid anchor and PT7 and RBS
Fusion protein of S-Layer SgsE with T7/lac and BisdA and BisdB
The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.
This is the B0010 part extracted by PCR from the doulbe terminator B0012. We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use! The characterization has been done both with T7 and Coli RNApolymerases.
I don't have any design considerations, anyway it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.
T7 promoter (strong promoter from T7 bacteriophage)
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
Wild-type found in T3 bacteriophage. Synthesized by IDT.
Fusion protein of S-Layer SbpA with T7/lac and BisdA and BisdB
DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag
S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS
T7 promoter with an T->G mutation at the -17 position of the consensus sequence
This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
S-layer cspB from Corynebacterium halotolerans with lipid anchor, PT7 and RBS
Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS
Gibson Assembly of BBa_E1010 and BBa_K525224
Fusion Protein of mRFP and S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS
Fusion Protein of S-Layer SbpA and LigA with His-Tag
SbpA was synthesized, LigA is the native ligase of E. coli.
Assembled with Freiburg Standard Assembly (RFC 25)
The sequence of T7 promoter is TAATACGACTCACTATA.
Two T7 promoters are reversely overlapping.
Fusion protein of SgsE, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
PT7 + RBS + SgsE || Red || BisdA || BisdB ; RCU 25
RBS(B0032)+T3 DNA-directed RNA polymerase
This part is the coding region of MerR, which is a mercury responsive regulator from Tn21.
We cloned this part from plasmid NR1, which was a gift from Anne O. Summers.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
T7 promoter with an A->C mutation at the -16 position of the consensus sequence
This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -10.
A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -10 base to a G, which should make it about 4% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
The -10 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an G, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
Two T7 promoters are reversely overlapping in two base pairs while a point mutation occurs at the 12th base pair, from A-T to T-A.
Conversion of the iGEM parts registry to SBOL2.1