//rnap/bacteriophage/t7
type: Collection
persistent identity: rnap bacteriophage t7
display id: rnap_bacteriophage_t7
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: cspB
member: BBa_K731722
member: CspB | RFP
member: BBa_Z0253
member: BBa_K113011
member: BBa_K525010
member: BBa_K113010
member: BBa_K525001
member: BBa_J34814
member: BBa_K525007
member: SbpA
member: BBa_R0186
member: BBa_R0182
member: BBa_K113012
member: BBa_K525008
member: BBa_K1614000
member: BBa_R0187
member: BBa_Z0252
member: LigA
member: pT7
member: BBa_R0183
member: BBa_J64997
member: BBa_K525009
member: BBa_I712074
member: BBa_Z0251
member: BBa_R0180
member: BBa_R0085
member: BBa_R0184
member: BBa_R0181
member: SgsE
member: BBa_R0185
member: BBa_K525002

BBa_R0187
type: ComponentDefinition
persistent identity: BBa R0187
display id: BBa_R0187
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an G, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_R0187_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962096
display id: annotation1962096
version: 1
top level: BBa_R0187
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0187/annotation1962096/range1962096/1

https://synbiohub.org/public/igem/BBa_R0187/annotation1962096/range1962096/1
type: Range
persistent identity: range1962096
display id: range1962096
version: 1
direction: direction/forward
top level: BBa_R0187
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1962097
display id: annotation1962097
version: 1
top level: BBa_R0187
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0187/annotation1962097/range1962097/1

https://synbiohub.org/public/igem/BBa_R0187/annotation1962097/range1962097/1
type: Range
persistent identity: range1962097
display id: range1962097
version: 1
direction: direction/forward
top level: BBa_R0187
start: 1
end: 20
orientation: inline

SbpA
type: ComponentDefinition
persistent identity: BBa K525403
display id: BBa_K525403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-Layer SbpA from Lysinibacillus sphaericus with T7/lac
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: S-Layer SgsE from Geobacillus stearothermophilus with T7/lac
mutable notes: Synthesis
mutable provenance: Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NgoMIV
sequence annotation: T7 promoter
sequence annotation: K525401
sequence annotation: SbpA domain
sequence annotation: ATG
sequence annotation: B0034
sequence annotation: AgeI
sequence annotation: lac operator
sequence annotation: TAA
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K525403_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129902
display id: annotation2129902
version: 1
top level: SbpA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129902/range2129902/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129902/range2129902/1
type: Range
persistent identity: range2129902
display id: range2129902
version: 1
direction: direction/forward
top level: SbpA
start: 76
end: 81
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2129896
display id: annotation2129896
version: 1
top level: SbpA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129896/range2129896/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129896/range2129896/1
type: Range
persistent identity: range2129896
display id: range2129896
version: 1
direction: direction/forward
top level: SbpA
start: 1
end: 20
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2129801
display id: annotation2129801
version: 1
top level: SbpA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129801/range2129801/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129801/range2129801/1
type: Range
persistent identity: range2129801
display id: range2129801
version: 1
direction: direction/forward
top level: SbpA
start: 73
end: 3201
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2129904
display id: annotation2129904
version: 1
top level: SbpA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129904/range2129904/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129904/range2129904/1
type: Range
persistent identity: range2129904
display id: range2129904
version: 1
direction: direction/forward
top level: SbpA
start: 73
end: 3201
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129900
display id: annotation2129900
version: 1
top level: SbpA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129900/range2129900/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129900/range2129900/1
type: Range
persistent identity: range2129900
display id: range2129900
version: 1
direction: direction/forward
top level: SbpA
start: 73
end: 75
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2129898
display id: annotation2129898
version: 1
top level: SbpA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129898/range2129898/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129898/range2129898/1
type: Range
persistent identity: range2129898
display id: range2129898
version: 1
direction: direction/forward
top level: SbpA
start: 53
end: 64
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129903
display id: annotation2129903
version: 1
top level: SbpA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129903/range2129903/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129903/range2129903/1
type: Range
persistent identity: range2129903
display id: range2129903
version: 1
direction: direction/forward
top level: SbpA
start: 3193
end: 3198
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2129897
display id: annotation2129897
version: 1
top level: SbpA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129897/range2129897/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129897/range2129897/1
type: Range
persistent identity: range2129897
display id: range2129897
version: 1
direction: direction/forward
top level: SbpA
start: 20
end: 44
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129901
display id: annotation2129901
version: 1
top level: SbpA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129901/range2129901/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129901/range2129901/1
type: Range
persistent identity: range2129901
display id: range2129901
version: 1
direction: direction/forward
top level: SbpA
start: 3199
end: 3201
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129899
display id: annotation2129899
version: 1
top level: SbpA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129899/range2129899/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129899/range2129899/1
type: Range
persistent identity: range2129899
display id: range2129899
version: 1
direction: direction/forward
top level: SbpA
start: 56
end: 59
orientation: inline

BBa_J64997
type: ComponentDefinition
persistent identity: BBa J64997
display id: BBa_J64997
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 consensus -10 and rest
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7-10
sequence: https://synbiohub.org/public/igem/BBa_J64997_sequence/1

T7-10
type: SequenceAnnotation
persistent identity: annotation1921746
display id: annotation1921746
version: 1
top level: BBa_J64997
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64997/annotation1921746/range1921746/1

https://synbiohub.org/public/igem/BBa_J64997/annotation1921746/range1921746/1
type: Range
persistent identity: range1921746
display id: range1921746
version: 1
direction: direction/forward
top level: BBa_J64997
start: 1
end: 19
orientation: inline

BBa_K1614000
type: ComponentDefinition
persistent identity: BBa K1614000
display id: BBa_K1614000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter for expression of functional RNA
creator: Stefan Holderbach
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T12:29:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 12380
owner id: 16422
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Test
mutable notes: None.
mutable provenance: T7 phage minimal promoter (consensus)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1614000_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2454728
display id: annotation2454728
version: 1
top level: BBa_K1614000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1

https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1
type: Range
persistent identity: range2454728
display id: range2454728
version: 1
direction: direction/forward
top level: BBa_K1614000
start: 1
end: 17
orientation: inline

BBa_R0180
type: ComponentDefinition
persistent identity: BBa R0180
display id: BBa_R0180
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->T mutation at the -16 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0180_sequence/1

cspB
type: ComponentDefinition
persistent identity: BBa K525123
display id: BBa_K525123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-layer cspB from Corynebacterium glutamicum with lipid anchor and PT7 and RBS
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: SL-BF-3 mit pT7 und RBS; Lipid anchor
mutable notes: PCR
mutable provenance: Brevibacterium flavum, PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: Lipid anchor
sequence annotation: S-layer
sequence annotation: Promoter
sequence: https://synbiohub.org/public/igem/BBa_K525123_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2127134
display id: annotation2127134
version: 1
top level: cspB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525123/annotation2127134/range2127134/1

https://synbiohub.org/public/igem/BBa_K525123/annotation2127134/range2127134/1
type: Range
persistent identity: range2127134
display id: range2127134
version: 1
direction: direction/forward
top level: cspB
start: 23
end: 34
orientation: inline

Lipid anchor
type: SequenceAnnotation
persistent identity: annotation2127316
display id: annotation2127316
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525123/annotation2127316/range2127316/1

https://synbiohub.org/public/igem/BBa_K525123/annotation2127316/range2127316/1
type: Range
persistent identity: range2127316
display id: range2127316
version: 1
direction: direction/forward
top level: cspB
start: 1348
end: 1423
orientation: inline

S-layer
type: SequenceAnnotation
persistent identity: annotation2127317
display id: annotation2127317
version: 1
top level: cspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525123/annotation2127317/range2127317/1

https://synbiohub.org/public/igem/BBa_K525123/annotation2127317/range2127317/1
type: Range
persistent identity: range2127317
display id: range2127317
version: 1
direction: direction/forward
top level: cspB
start: 35
end: 1347
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2128108
display id: annotation2128108
version: 1
top level: cspB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525123/annotation2128108/range2128108/1

https://synbiohub.org/public/igem/BBa_K525123/annotation2128108/range2128108/1
type: Range
persistent identity: range2128108
display id: range2128108
version: 1
direction: direction/forward
top level: cspB
start: 1
end: 22
orientation: inline

BBa_K525010
type: ComponentDefinition
persistent identity: BBa K525010
display id: BBa_K525010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and LigA with His-Tag
creator: Manuel Wittchen
created: 2011-10-24T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
group u list: _690_
m user id: 0
owner id: 8718
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion Protein of S-Layer SbpA and LigA with His-Tag
mutable notes: Assembled with Freiburg Standard Assembly (RFC 25)
mutable provenance: SbpA was synthesized, LigA is the native ligase of E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SbpA domain
sequence annotation: lac operator
sequence annotation: B0034
sequence annotation: T7 Promotor
sequence annotation: LigA domain
sequence annotation: NgoMIV
sequence annotation: Freiburg scar
sequence annotation: ATG
sequence annotation: His-Tag
sequence annotation: K525740
sequence annotation: K525403
sequence: https://synbiohub.org/public/igem/BBa_K525010_sequence/1

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2160524
display id: annotation2160524
version: 1
top level: BBa_K525010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160524/range2160524/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160524/range2160524/1
type: Range
persistent identity: range2160524
display id: range2160524
version: 1
direction: direction/forward
top level: BBa_K525010
start: 73
end: 3202
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160520
display id: annotation2160520
version: 1
top level: BBa_K525010
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160520/range2160520/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160520/range2160520/1
type: Range
persistent identity: range2160520
display id: range2160520
version: 1
direction: direction/forward
top level: BBa_K525010
start: 20
end: 44
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160521
display id: annotation2160521
version: 1
top level: BBa_K525010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160521/range2160521/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160521/range2160521/1
type: Range
persistent identity: range2160521
display id: range2160521
version: 1
direction: direction/forward
top level: BBa_K525010
start: 53
end: 64
orientation: inline

T7 Promotor
type: SequenceAnnotation
persistent identity: annotation2160519
display id: annotation2160519
version: 1
top level: BBa_K525010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160519/range2160519/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160519/range2160519/1
type: Range
persistent identity: range2160519
display id: range2160519
version: 1
direction: direction/forward
top level: BBa_K525010
start: 1
end: 20
orientation: inline

LigA domain
type: SequenceAnnotation
persistent identity: annotation2160525
display id: annotation2160525
version: 1
top level: BBa_K525010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160525/range2160525/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160525/range2160525/1
type: Range
persistent identity: range2160525
display id: range2160525
version: 1
direction: direction/forward
top level: BBa_K525010
start: 3199
end: 5208
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160529
display id: annotation2160529
version: 1
top level: BBa_K525010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160529/range2160529/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160529/range2160529/1
type: Range
persistent identity: range2160529
display id: range2160529
version: 1
direction: direction/forward
top level: BBa_K525010
start: 76
end: 81
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160523
display id: annotation2160523
version: 1
top level: BBa_K525010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160523/range2160523/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160523/range2160523/1
type: Range
persistent identity: range2160523
display id: range2160523
version: 1
direction: direction/forward
top level: BBa_K525010
start: 3193
end: 3199
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160522
display id: annotation2160522
version: 1
top level: BBa_K525010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160522/range2160522/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160522/range2160522/1
type: Range
persistent identity: range2160522
display id: range2160522
version: 1
direction: direction/forward
top level: BBa_K525010
start: 73
end: 75
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2160526
display id: annotation2160526
version: 1
top level: BBa_K525010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160526/range2160526/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160526/range2160526/1
type: Range
persistent identity: range2160526
display id: range2160526
version: 1
direction: direction/forward
top level: BBa_K525010
start: 5209
end: 5226
orientation: inline

K525740
type: SequenceAnnotation
persistent identity: annotation2160528
display id: annotation2160528
version: 1
top level: BBa_K525010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160528/range2160528/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160528/range2160528/1
type: Range
persistent identity: range2160528
display id: range2160528
version: 1
direction: direction/forward
top level: BBa_K525010
start: 3189
end: 5226
orientation: inline

K525403
type: SequenceAnnotation
persistent identity: annotation2160527
display id: annotation2160527
version: 1
top level: BBa_K525010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160527/range2160527/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160527/range2160527/1
type: Range
persistent identity: range2160527
display id: range2160527
version: 1
direction: direction/forward
top level: BBa_K525010
start: 1
end: 3193
orientation: inline

BBa_K113011
type: ComponentDefinition
persistent identity: BBa K113011
display id: BBa_K113011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: more overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping in five base pairs, with the third one mutated from A to G.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113011_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989359
display id: annotation1989359
version: 1
top level: BBa_K113011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113011/annotation1989359/range1989359/1

https://synbiohub.org/public/igem/BBa_K113011/annotation1989359/range1989359/1
type: Range
persistent identity: range1989359
display id: range1989359
version: 1
direction: direction/reverse
top level: BBa_K113011
start: 4
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989380
display id: annotation1989380
version: 1
top level: BBa_K113011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113011/annotation1989380/range1989380/1

https://synbiohub.org/public/igem/BBa_K113011/annotation1989380/range1989380/1
type: Range
persistent identity: range1989380
display id: range1989380
version: 1
direction: direction/forward
top level: BBa_K113011
start: 17
end: 34
orientation: inline

CspB | RFP
type: ComponentDefinition
persistent identity: BBa K525234
display id: BBa_K525234
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS
creator: Timo Wolf
created: 2011-09-19T11:00:00Z
modified: 2016-01-25T01:34:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion Protein of mRFP and S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS
mutable notes: Gibson Assembly of BBa_E1010 and BBa_K525224
mutable provenance: Gibson Assembly of BBa_E1010 and BBa_K525224
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: K525222
sequence annotation: mRFP
sequence annotation: BBa_B0034
sequence annotation: RBS
sequence annotation: BBa_E1010
sequence annotation: TAA
sequence annotation: Linker
sequence annotation: TAT-sequence
sequence annotation: cspB domain
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K525234_sequence/1

K525222
type: SequenceAnnotation
persistent identity: annotation2161282
display id: annotation2161282
version: 1
top level: CspB | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161282/range2161282/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161282/range2161282/1
type: Range
persistent identity: range2161282
display id: range2161282
version: 1
direction: direction/forward
top level: CspB | RFP
start: 809
end: 2189
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2161287
display id: annotation2161287
version: 1
top level: CspB | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161287/range2161287/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161287/range2161287/1
type: Range
persistent identity: range2161287
display id: range2161287
version: 1
direction: direction/forward
top level: CspB | RFP
start: 129
end: 799
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2161284
display id: annotation2161284
version: 1
top level: CspB | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161284/range2161284/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161284/range2161284/1
type: Range
persistent identity: range2161284
display id: range2161284
version: 1
direction: direction/forward
top level: CspB | RFP
start: 21
end: 33
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161285
display id: annotation2161285
version: 1
top level: CspB | RFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161285/range2161285/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161285/range2161285/1
type: Range
persistent identity: range2161285
display id: range2161285
version: 1
direction: direction/forward
top level: CspB | RFP
start: 25
end: 28
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2161288
display id: annotation2161288
version: 1
top level: CspB | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161288/range2161288/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161288/range2161288/1
type: Range
persistent identity: range2161288
display id: range2161288
version: 1
direction: direction/forward
top level: CspB | RFP
start: 129
end: 799
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161291
display id: annotation2161291
version: 1
top level: CspB | RFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161291/range2161291/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161291/range2161291/1
type: Range
persistent identity: range2161291
display id: range2161291
version: 1
direction: direction/forward
top level: CspB | RFP
start: 2187
end: 2189
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2161289
display id: annotation2161289
version: 1
top level: CspB | RFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161289/range2161289/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161289/range2161289/1
type: Range
persistent identity: range2161289
display id: range2161289
version: 1
direction: direction/forward
top level: CspB | RFP
start: 800
end: 808
orientation: inline

TAT-sequence
type: SequenceAnnotation
persistent identity: annotation2161286
display id: annotation2161286
version: 1
top level: CspB | RFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161286/range2161286/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161286/range2161286/1
type: Range
persistent identity: range2161286
display id: range2161286
version: 1
direction: direction/forward
top level: CspB | RFP
start: 34
end: 128
orientation: inline

cspB domain
type: SequenceAnnotation
persistent identity: annotation2161290
display id: annotation2161290
version: 1
top level: CspB | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161290/range2161290/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161290/range2161290/1
type: Range
persistent identity: range2161290
display id: range2161290
version: 1
direction: direction/forward
top level: CspB | RFP
start: 809
end: 2189
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2161283
display id: annotation2161283
version: 1
top level: CspB | RFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161283/range2161283/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161283/range2161283/1
type: Range
persistent identity: range2161283
display id: range2161283
version: 1
direction: direction/forward
top level: CspB | RFP
start: 1
end: 20
orientation: inline

BBa_R0185
type: ComponentDefinition
persistent identity: BBa R0185
display id: BBa_R0185
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -10 base to a G, which should make it about 4% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -10 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -10.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: T7 promoter sequence
sequence: https://synbiohub.org/public/igem/BBa_R0185_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962093
display id: annotation1962093
version: 1
top level: BBa_R0185
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0185/annotation1962093/range1962093/1

https://synbiohub.org/public/igem/BBa_R0185/annotation1962093/range1962093/1
type: Range
persistent identity: range1962093
display id: range1962093
version: 1
direction: direction/forward
top level: BBa_R0185
start: 20
end: 44
orientation: inline

T7 promoter sequence
type: SequenceAnnotation
persistent identity: annotation1962092
display id: annotation1962092
version: 1
top level: BBa_R0185
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0185/annotation1962092/range1962092/1

https://synbiohub.org/public/igem/BBa_R0185/annotation1962092/range1962092/1
type: Range
persistent identity: range1962092
display id: range1962092
version: 1
direction: direction/forward
top level: BBa_R0185
start: 1
end: 20
orientation: inline

cspB
type: ComponentDefinition
persistent identity: BBa K525221
display id: BBa_K525221
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-layer cspB from Corynebacterium halotolerans with TAT-Sequence and lipid anchor, PT7 and RBS
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: pT7 + RBS + S-Layer + TAT-Sequenz + Lipidanker
mutable notes: PCR
mutable provenance: Corynebacterium halotolerans
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Promoter
sequence annotation: RBS
sequence annotation: TAT-Sequence
sequence annotation: Lipid anchor
sequence annotation: S-layer
sequence: https://synbiohub.org/public/igem/BBa_K525221_sequence/1

Promoter
type: SequenceAnnotation
persistent identity: annotation2128109
display id: annotation2128109
version: 1
top level: cspB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525221/annotation2128109/range2128109/1

https://synbiohub.org/public/igem/BBa_K525221/annotation2128109/range2128109/1
type: Range
persistent identity: range2128109
display id: range2128109
version: 1
direction: direction/forward
top level: cspB
start: 1
end: 22
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2127399
display id: annotation2127399
version: 1
top level: cspB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525221/annotation2127399/range2127399/1

https://synbiohub.org/public/igem/BBa_K525221/annotation2127399/range2127399/1
type: Range
persistent identity: range2127399
display id: range2127399
version: 1
direction: direction/forward
top level: cspB
start: 23
end: 34
orientation: inline

TAT-Sequence
type: SequenceAnnotation
persistent identity: annotation2127314
display id: annotation2127314
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525221/annotation2127314/range2127314/1

https://synbiohub.org/public/igem/BBa_K525221/annotation2127314/range2127314/1
type: Range
persistent identity: range2127314
display id: range2127314
version: 1
direction: direction/forward
top level: cspB
start: 35
end: 123
orientation: inline

Lipid anchor
type: SequenceAnnotation
persistent identity: annotation2127315
display id: annotation2127315
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525221/annotation2127315/range2127315/1

https://synbiohub.org/public/igem/BBa_K525221/annotation2127315/range2127315/1
type: Range
persistent identity: range2127315
display id: range2127315
version: 1
direction: direction/forward
top level: cspB
start: 1502
end: 1582
orientation: inline

S-layer
type: SequenceAnnotation
persistent identity: annotation2127392
display id: annotation2127392
version: 1
top level: cspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525221/annotation2127392/range2127392/1

https://synbiohub.org/public/igem/BBa_K525221/annotation2127392/range2127392/1
type: Range
persistent identity: range2127392
display id: range2127392
version: 1
direction: direction/forward
top level: cspB
start: 124
end: 1501
orientation: inline

BBa_R0186
type: ComponentDefinition
persistent identity: BBa R0186
display id: BBa_R0186
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an A, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_R0186_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962094
display id: annotation1962094
version: 1
top level: BBa_R0186
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0186/annotation1962094/range1962094/1

https://synbiohub.org/public/igem/BBa_R0186/annotation1962094/range1962094/1
type: Range
persistent identity: range1962094
display id: range1962094
version: 1
direction: direction/forward
top level: BBa_R0186
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1962095
display id: annotation1962095
version: 1
top level: BBa_R0186
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0186/annotation1962095/range1962095/1

https://synbiohub.org/public/igem/BBa_R0186/annotation1962095/range1962095/1
type: Range
persistent identity: range1962095
display id: range1962095
version: 1
direction: direction/forward
top level: BBa_R0186
start: 1
end: 20
orientation: inline

BBa_Z0251
type: ComponentDefinition
persistent identity: BBa Z0251
display id: BBa_Z0251
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 strong promoter
creator: T7.2
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for T7 RNA polymerase
mutable provenance: bacteriophage T7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_Z0251_sequence/1

consensus sequence
type: SequenceAnnotation
persistent identity: annotation1476333
display id: annotation1476333
version: 1
top level: BBa_Z0251
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1

https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1
type: Range
persistent identity: range1476333
display id: range1476333
version: 1
direction: direction/forward
top level: BBa_Z0251
start: 6
end: 28
orientation: inline

BBa_R0181
type: ComponentDefinition
persistent identity: BBa R0181
display id: BBa_R0181
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an T->G mutation at the -17 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0181_sequence/1

BBa_R0183
type: ComponentDefinition
persistent identity: BBa R0183
display id: BBa_R0183
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->C mutation at the -16 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0183_sequence/1

BBa_K525001
type: ComponentDefinition
persistent identity: BBa K525001
display id: BBa_K525001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SgsE, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SgsE || Red || BisdA || BisdB ; RCU 25
mutable notes: Assembled RCU 25
mutable provenance: BBa_K525303, BBa_K525499, BBa_K123000, BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Freiburg Scar
sequence annotation: RBS
sequence annotation: K525301
sequence annotation: B0034
sequence annotation: SgsE domain
sequence annotation: Reductase
sequence annotation: BisdB
sequence annotation: ATG
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: TAA
sequence annotation: BisdA
sequence annotation: AgeI
sequence annotation: K525560
sequence: https://synbiohub.org/public/igem/BBa_K525001_sequence/1

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160965
display id: annotation2160965
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160965/range2160965/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160965/range2160965/1
type: Range
persistent identity: range2160965
display id: range2160965
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3148
end: 3153
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160963
display id: annotation2160963
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160963/range2160963/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160963/range2160963/1
type: Range
persistent identity: range2160963
display id: range2160963
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2401
end: 2406
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2160960
display id: annotation2160960
version: 1
top level: BBa_K525001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160960/range2160960/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160960/range2160960/1
type: Range
persistent identity: range2160960
display id: range2160960
version: 1
direction: direction/forward
top level: BBa_K525001
start: 56
end: 59
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2160962
display id: annotation2160962
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160962/range2160962/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160962/range2160962/1
type: Range
persistent identity: range2160962
display id: range2160962
version: 1
direction: direction/forward
top level: BBa_K525001
start: 73
end: 2400
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160959
display id: annotation2160959
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160959/range2160959/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160959/range2160959/1
type: Range
persistent identity: range2160959
display id: range2160959
version: 1
direction: direction/forward
top level: BBa_K525001
start: 53
end: 64
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2161004
display id: annotation2161004
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161004/range2161004/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161004/range2161004/1
type: Range
persistent identity: range2161004
display id: range2161004
version: 1
direction: direction/forward
top level: BBa_K525001
start: 76
end: 2400
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2161003
display id: annotation2161003
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161003/range2161003/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161003/range2161003/1
type: Range
persistent identity: range2161003
display id: range2161003
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2407
end: 3147
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160989
display id: annotation2160989
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160989/range2160989/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160989/range2160989/1
type: Range
persistent identity: range2160989
display id: range2160989
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3466
end: 3471
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2160992
display id: annotation2160992
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160992/range2160992/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160992/range2160992/1
type: Range
persistent identity: range2160992
display id: range2160992
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3472
end: 4746
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160961
display id: annotation2160961
version: 1
top level: BBa_K525001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160961/range2160961/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160961/range2160961/1
type: Range
persistent identity: range2160961
display id: range2160961
version: 1
direction: direction/forward
top level: BBa_K525001
start: 73
end: 75
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160958
display id: annotation2160958
version: 1
top level: BBa_K525001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160958/range2160958/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160958/range2160958/1
type: Range
persistent identity: range2160958
display id: range2160958
version: 1
direction: direction/forward
top level: BBa_K525001
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2160957
display id: annotation2160957
version: 1
top level: BBa_K525001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160957/range2160957/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160957/range2160957/1
type: Range
persistent identity: range2160957
display id: range2160957
version: 1
direction: direction/forward
top level: BBa_K525001
start: 1
end: 20
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160990
display id: annotation2160990
version: 1
top level: BBa_K525001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160990/range2160990/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160990/range2160990/1
type: Range
persistent identity: range2160990
display id: range2160990
version: 1
direction: direction/forward
top level: BBa_K525001
start: 4744
end: 4746
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161002
display id: annotation2161002
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161002/range2161002/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161002/range2161002/1
type: Range
persistent identity: range2161002
display id: range2161002
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3154
end: 3465
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160991
display id: annotation2160991
version: 1
top level: BBa_K525001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160991/range2160991/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160991/range2160991/1
type: Range
persistent identity: range2160991
display id: range2160991
version: 1
direction: direction/forward
top level: BBa_K525001
start: 4738
end: 4743
orientation: inline

K525560
type: SequenceAnnotation
persistent identity: annotation2160964
display id: annotation2160964
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160964/range2160964/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160964/range2160964/1
type: Range
persistent identity: range2160964
display id: range2160964
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2407
end: 4746
orientation: inline

BBa_R0184
type: ComponentDefinition
persistent identity: BBa R0184
display id: BBa_R0184
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2007-04-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
mutable notes: The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Center of binding site symmetry
sequence annotation: lacI binding site
sequence annotation: BBa_R0183 fragment
sequence: https://synbiohub.org/public/igem/BBa_R0184_sequence/1

Center of binding site symmetry
type: SequenceAnnotation
persistent identity: annotation1932680
display id: annotation1932680
version: 1
top level: BBa_R0184
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
type: Range
persistent identity: range1932680
display id: range1932680
version: 1
direction: direction/forward
top level: BBa_R0184
start: 31
end: 31
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1932679
display id: annotation1932679
version: 1
top level: BBa_R0184
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
type: Range
persistent identity: range1932679
display id: range1932679
version: 1
direction: direction/forward
top level: BBa_R0184
start: 20
end: 44
orientation: inline

BBa_R0183 fragment
type: SequenceAnnotation
persistent identity: annotation1932678
display id: annotation1932678
version: 1
top level: BBa_R0184
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
type: Range
persistent identity: range1932678
display id: range1932678
version: 1
direction: direction/forward
top level: BBa_R0184
start: 1
end: 20
orientation: inline

BBa_Z0253
type: ComponentDefinition
persistent identity: BBa Z0253
display id: BBa_Z0253
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 weak binding promoter
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ag->TA from concensus
sequence annotation: ag->GA from concensus
sequence: https://synbiohub.org/public/igem/BBa_Z0253_sequence/1

ag->TA from concensus
type: SequenceAnnotation
persistent identity: annotation1862684
display id: annotation1862684
version: 1
top level: BBa_Z0253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1
type: Range
persistent identity: range1862684
display id: range1862684
version: 1
direction: direction/forward
top level: BBa_Z0253
start: 27
end: 28
orientation: inline

ag->GA from concensus
type: SequenceAnnotation
persistent identity: annotation1862691
display id: annotation1862691
version: 1
top level: BBa_Z0253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1
type: Range
persistent identity: range1862691
display id: range1862691
version: 1
direction: direction/forward
top level: BBa_Z0253
start: 31
end: 32
orientation: inline

BBa_R0182
type: ComponentDefinition
persistent identity: BBa R0182
display id: BBa_R0182
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->T mutation at the -10 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0182_sequence/1

pT7
type: ComponentDefinition
persistent identity: BBa I719005
display id: BBa_I719005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter
creator: Imperial 2007
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _128_
m user id: 0
owner id: 2097
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Just a T7 Promoter
mutable notes: None
mutable provenance: ---
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I719005_sequence/1

BBa_K731722
type: ComponentDefinition
persistent identity: BBa K731722
display id: BBa_K731722
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T1 terminator from E. coli rrnB
creator: Anna Depetris, Giacomo Giacomelli
created: 2012-08-22T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12063
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the B0010 part extracted by PCR from the doulbe terminator B0012. We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use! The characterization has been done both with T7 and Coli RNApolymerases.
mutable notes: I don't have any design considerations, anyway it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.
mutable provenance: This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0010
sequence: https://synbiohub.org/public/igem/BBa_K731722_sequence/1

B0010
type: SequenceAnnotation
persistent identity: annotation2180631
display id: annotation2180631
version: 1
top level: BBa_K731722
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K731722/annotation2180631/range2180631/1

https://synbiohub.org/public/igem/BBa_K731722/annotation2180631/range2180631/1
type: Range
persistent identity: range2180631
display id: range2180631
version: 1
direction: direction/forward
top level: BBa_K731722
start: 1
end: 79
orientation: inline

BBa_K113010
type: ComponentDefinition
persistent identity: BBa K113010
display id: BBa_K113010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113010_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989277
display id: annotation1989277
version: 1
top level: BBa_K113010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113010/annotation1989277/range1989277/1

https://synbiohub.org/public/igem/BBa_K113010/annotation1989277/range1989277/1
type: Range
persistent identity: range1989277
display id: range1989277
version: 1
direction: direction/reverse
top level: BBa_K113010
start: 4
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989307
display id: annotation1989307
version: 1
top level: BBa_K113010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113010/annotation1989307/range1989307/1

https://synbiohub.org/public/igem/BBa_K113010/annotation1989307/range1989307/1
type: Range
persistent identity: range1989307
display id: range1989307
version: 1
direction: direction/forward
top level: BBa_K113010
start: 20
end: 37
orientation: inline

BBa_I712074
type: ComponentDefinition
persistent identity: BBa I712074
display id: BBa_I712074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (strong promoter from T7 bacteriophage)
creator: Rok Gaber
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712074_sequence/1

BBa_Z0252
type: ComponentDefinition
persistent identity: BBa Z0252
display id: BBa_Z0252
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 weak binding and processivity
creator: T7.2
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for T7 RNA polymerase
mutable provenance: T7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: 23bp conserved region
sequence annotation: t -> a mutation from consensus
sequence annotation: tga -> acg mutation from consensus
sequence: https://synbiohub.org/public/igem/BBa_Z0252_sequence/1

23bp conserved region
type: SequenceAnnotation
persistent identity: annotation1476343
display id: annotation1476343
version: 1
top level: BBa_Z0252
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1
type: Range
persistent identity: range1476343
display id: range1476343
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 6
end: 28
orientation: inline

t -> a mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476345
display id: annotation1476345
version: 1
top level: BBa_Z0252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1
type: Range
persistent identity: range1476345
display id: range1476345
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 21
end: 21
orientation: inline

tga -> acg mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476344
display id: annotation1476344
version: 1
top level: BBa_Z0252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1
type: Range
persistent identity: range1476344
display id: range1476344
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 10
end: 12
orientation: inline

BBa_K525002
type: ComponentDefinition
persistent identity: BBa K525002
display id: BBa_K525002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SbpA || Red || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525403; BBa_K525499; BBa_K123000 ; BBa_K123001 ; RCU 25
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: K525560
sequence annotation: Freiburg sacr
sequence annotation: Freiburg scar
sequence annotation: RBS
sequence annotation: SbpA domain
sequence annotation: Reductase
sequence annotation: B0034
sequence annotation: T7 promoter
sequence annotation: BisdA
sequence annotation: BisdB
sequence annotation: K525401
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence annotation: ATG
sequence annotation: TAA
sequence annotation: lac operator
sequence: https://synbiohub.org/public/igem/BBa_K525002_sequence/1

K525560
type: SequenceAnnotation
persistent identity: annotation2160929
display id: annotation2160929
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160929/range2160929/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160929/range2160929/1
type: Range
persistent identity: range2160929
display id: range2160929
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3199
end: 5538
orientation: inline

Freiburg sacr
type: SequenceAnnotation
persistent identity: annotation2160932
display id: annotation2160932
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160932/range2160932/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160932/range2160932/1
type: Range
persistent identity: range2160932
display id: range2160932
version: 1
direction: direction/forward
top level: BBa_K525002
start: 4258
end: 4262
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160927
display id: annotation2160927
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160927/range2160927/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160927/range2160927/1
type: Range
persistent identity: range2160927
display id: range2160927
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3193
end: 3198
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2160923
display id: annotation2160923
version: 1
top level: BBa_K525002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160923/range2160923/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160923/range2160923/1
type: Range
persistent identity: range2160923
display id: range2160923
version: 1
direction: direction/forward
top level: BBa_K525002
start: 56
end: 59
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2160925
display id: annotation2160925
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160925/range2160925/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160925/range2160925/1
type: Range
persistent identity: range2160925
display id: range2160925
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 3192
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2160928
display id: annotation2160928
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160928/range2160928/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160928/range2160928/1
type: Range
persistent identity: range2160928
display id: range2160928
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3199
end: 3939
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161005
display id: annotation2161005
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2161005/range2161005/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2161005/range2161005/1
type: Range
persistent identity: range2161005
display id: range2161005
version: 1
direction: direction/forward
top level: BBa_K525002
start: 53
end: 64
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160930
display id: annotation2160930
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160930/range2160930/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160930/range2160930/1
type: Range
persistent identity: range2160930
display id: range2160930
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3940
end: 3945
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2160920
display id: annotation2160920
version: 1
top level: BBa_K525002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160920/range2160920/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160920/range2160920/1
type: Range
persistent identity: range2160920
display id: range2160920
version: 1
direction: direction/forward
top level: BBa_K525002
start: 1
end: 20
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2160931
display id: annotation2160931
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160931/range2160931/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160931/range2160931/1
type: Range
persistent identity: range2160931
display id: range2160931
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3946
end: 4258
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2160933
display id: annotation2160933
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160933/range2160933/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160933/range2160933/1
type: Range
persistent identity: range2160933
display id: range2160933
version: 1
direction: direction/forward
top level: BBa_K525002
start: 4263
end: 5538
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2160921
display id: annotation2160921
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160921/range2160921/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160921/range2160921/1
type: Range
persistent identity: range2160921
display id: range2160921
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 3192
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160934
display id: annotation2160934
version: 1
top level: BBa_K525002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160934/range2160934/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160934/range2160934/1
type: Range
persistent identity: range2160934
display id: range2160934
version: 1
direction: direction/forward
top level: BBa_K525002
start: 5530
end: 5535
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160926
display id: annotation2160926
version: 1
top level: BBa_K525002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160926/range2160926/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160926/range2160926/1
type: Range
persistent identity: range2160926
display id: range2160926
version: 1
direction: direction/forward
top level: BBa_K525002
start: 76
end: 81
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160924
display id: annotation2160924
version: 1
top level: BBa_K525002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160924/range2160924/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160924/range2160924/1
type: Range
persistent identity: range2160924
display id: range2160924
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 75
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160935
display id: annotation2160935
version: 1
top level: BBa_K525002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160935/range2160935/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160935/range2160935/1
type: Range
persistent identity: range2160935
display id: range2160935
version: 1
direction: direction/forward
top level: BBa_K525002
start: 5536
end: 5538
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160922
display id: annotation2160922
version: 1
top level: BBa_K525002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160922/range2160922/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160922/range2160922/1
type: Range
persistent identity: range2160922
display id: range2160922
version: 1
direction: direction/forward
top level: BBa_K525002
start: 20
end: 40
orientation: inline

cspB
type: ComponentDefinition
persistent identity: BBa K525121
display id: BBa_K525121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SL-BF-1 mit pT7 und RBS
mutable notes: PCR
mutable provenance: Brevibacterum flavum
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAT-sequence
sequence annotation: RBS
sequence annotation: S-layer
sequence annotation: C to A
sequence annotation: Promoter
sequence annotation: lipid anchor
sequence: https://synbiohub.org/public/igem/BBa_K525121_sequence/1

TAT-sequence
type: SequenceAnnotation
persistent identity: annotation2128074
display id: annotation2128074
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2128074/range2128074/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2128074/range2128074/1
type: Range
persistent identity: range2128074
display id: range2128074
version: 1
direction: direction/forward
top level: cspB
start: 35
end: 124
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2127133
display id: annotation2127133
version: 1
top level: cspB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2127133/range2127133/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2127133/range2127133/1
type: Range
persistent identity: range2127133
display id: range2127133
version: 1
direction: direction/forward
top level: cspB
start: 23
end: 34
orientation: inline

S-layer
type: SequenceAnnotation
persistent identity: annotation2128145
display id: annotation2128145
version: 1
top level: cspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2128145/range2128145/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2128145/range2128145/1
type: Range
persistent identity: range2128145
display id: range2128145
version: 1
direction: direction/forward
top level: cspB
start: 125
end: 1423
orientation: inline

C to A
type: SequenceAnnotation
persistent identity: annotation2137388
display id: annotation2137388
version: 1
top level: cspB
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2137388/range2137388/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2137388/range2137388/1
type: Range
persistent identity: range2137388
display id: range2137388
version: 1
direction: direction/forward
top level: cspB
start: 880
end: 880
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2128075
display id: annotation2128075
version: 1
top level: cspB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2128075/range2128075/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2128075/range2128075/1
type: Range
persistent identity: range2128075
display id: range2128075
version: 1
direction: direction/forward
top level: cspB
start: 1
end: 22
orientation: inline

lipid anchor
type: SequenceAnnotation
persistent identity: annotation2128144
display id: annotation2128144
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2128144/range2128144/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2128144/range2128144/1
type: Range
persistent identity: range2128144
display id: range2128144
version: 1
direction: direction/forward
top level: cspB
start: 1424
end: 1510
orientation: inline

BBa_K525008
type: ComponentDefinition
persistent identity: BBa K525008
display id: BBa_K525008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of S-Layer SbpA with T7/lac and BisdA and BisdB
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PT7 + RBS + SbpA || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525403 , BBa_K123000 , BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BisdB
sequence annotation: ATG
sequence annotation: NgoMIV
sequence annotation: T7
sequence annotation: SbpA domain
sequence annotation: RBS
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: B0034
sequence annotation: Freiburg Scar
sequence annotation: lac operator
sequence annotation: BisdA
sequence annotation: K525401
sequence annotation: K525515
sequence: https://synbiohub.org/public/igem/BBa_K525008_sequence/1

BisdB
type: SequenceAnnotation
persistent identity: annotation2161205
display id: annotation2161205
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161205/range2161205/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161205/range2161205/1
type: Range
persistent identity: range2161205
display id: range2161205
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3517
end: 4791
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161199
display id: annotation2161199
version: 1
top level: BBa_K525008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161199/range2161199/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161199/range2161199/1
type: Range
persistent identity: range2161199
display id: range2161199
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 75
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161201
display id: annotation2161201
version: 1
top level: BBa_K525008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161201/range2161201/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161201/range2161201/1
type: Range
persistent identity: range2161201
display id: range2161201
version: 1
direction: direction/forward
top level: BBa_K525008
start: 76
end: 82
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2161194
display id: annotation2161194
version: 1
top level: BBa_K525008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161194/range2161194/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161194/range2161194/1
type: Range
persistent identity: range2161194
display id: range2161194
version: 1
direction: direction/forward
top level: BBa_K525008
start: 1
end: 20
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2161200
display id: annotation2161200
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161200/range2161200/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161200/range2161200/1
type: Range
persistent identity: range2161200
display id: range2161200
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 3192
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161197
display id: annotation2161197
version: 1
top level: BBa_K525008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161197/range2161197/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161197/range2161197/1
type: Range
persistent identity: range2161197
display id: range2161197
version: 1
direction: direction/forward
top level: BBa_K525008
start: 56
end: 59
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161206
display id: annotation2161206
version: 1
top level: BBa_K525008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161206/range2161206/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161206/range2161206/1
type: Range
persistent identity: range2161206
display id: range2161206
version: 1
direction: direction/forward
top level: BBa_K525008
start: 4789
end: 4791
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161208
display id: annotation2161208
version: 1
top level: BBa_K525008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161208/range2161208/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161208/range2161208/1
type: Range
persistent identity: range2161208
display id: range2161208
version: 1
direction: direction/forward
top level: BBa_K525008
start: 4783
end: 4788
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161196
display id: annotation2161196
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161196/range2161196/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161196/range2161196/1
type: Range
persistent identity: range2161196
display id: range2161196
version: 1
direction: direction/forward
top level: BBa_K525008
start: 53
end: 64
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161204
display id: annotation2161204
version: 1
top level: BBa_K525008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161204/range2161204/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161204/range2161204/1
type: Range
persistent identity: range2161204
display id: range2161204
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3511
end: 3516
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2161195
display id: annotation2161195
version: 1
top level: BBa_K525008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161195/range2161195/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161195/range2161195/1
type: Range
persistent identity: range2161195
display id: range2161195
version: 1
direction: direction/forward
top level: BBa_K525008
start: 20
end: 44
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161203
display id: annotation2161203
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161203/range2161203/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161203/range2161203/1
type: Range
persistent identity: range2161203
display id: range2161203
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3199
end: 3510
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2161198
display id: annotation2161198
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161198/range2161198/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161198/range2161198/1
type: Range
persistent identity: range2161198
display id: range2161198
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 3192
orientation: inline

K525515
type: SequenceAnnotation
persistent identity: annotation2161207
display id: annotation2161207
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161207/range2161207/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161207/range2161207/1
type: Range
persistent identity: range2161207
display id: range2161207
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3199
end: 4791
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161202
display id: annotation2161202
version: 1
top level: BBa_K525008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161202/range2161202/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161202/range2161202/1
type: Range
persistent identity: range2161202
display id: range2161202
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3193
end: 3198
orientation: inline

SgsE
type: ComponentDefinition
persistent identity: BBa K525303
display id: BBa_K525303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-Layer SgsE from Geobacillus stearothermophilus with T7/lac
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: S-Layer SgsE from Geobacillus stearothermophilus
mutable notes: Synthesis
mutable provenance: Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: NgoMIV
sequence annotation: K525301
sequence annotation: TAA
sequence annotation: RBS
sequence annotation: B0034
sequence annotation: AgeI
sequence annotation: SgsE domain
sequence: https://synbiohub.org/public/igem/BBa_K525303_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2129997
display id: annotation2129997
version: 1
top level: SgsE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129997/range2129997/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129997/range2129997/1
type: Range
persistent identity: range2129997
display id: range2129997
version: 1
direction: direction/forward
top level: SgsE
start: 73
end: 75
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2129994
display id: annotation2129994
version: 1
top level: SgsE
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129994/range2129994/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129994/range2129994/1
type: Range
persistent identity: range2129994
display id: range2129994
version: 1
direction: direction/forward
top level: SgsE
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2128160
display id: annotation2128160
version: 1
top level: SgsE
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2128160/range2128160/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2128160/range2128160/1
type: Range
persistent identity: range2128160
display id: range2128160
version: 1
direction: direction/forward
top level: SgsE
start: 1
end: 20
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129998
display id: annotation2129998
version: 1
top level: SgsE
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129998/range2129998/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129998/range2129998/1
type: Range
persistent identity: range2129998
display id: range2129998
version: 1
direction: direction/forward
top level: SgsE
start: 76
end: 81
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2128153
display id: annotation2128153
version: 1
top level: SgsE
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2128153/range2128153/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2128153/range2128153/1
type: Range
persistent identity: range2128153
display id: range2128153
version: 1
direction: direction/forward
top level: SgsE
start: 73
end: 2409
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129999
display id: annotation2129999
version: 1
top level: SgsE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129999/range2129999/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129999/range2129999/1
type: Range
persistent identity: range2129999
display id: range2129999
version: 1
direction: direction/forward
top level: SgsE
start: 2407
end: 2409
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129995
display id: annotation2129995
version: 1
top level: SgsE
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129995/range2129995/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129995/range2129995/1
type: Range
persistent identity: range2129995
display id: range2129995
version: 1
direction: direction/forward
top level: SgsE
start: 56
end: 59
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2128152
display id: annotation2128152
version: 1
top level: SgsE
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2128152/range2128152/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2128152/range2128152/1
type: Range
persistent identity: range2128152
display id: range2128152
version: 1
direction: direction/forward
top level: SgsE
start: 53
end: 64
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2130000
display id: annotation2130000
version: 1
top level: SgsE
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2130000/range2130000/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2130000/range2130000/1
type: Range
persistent identity: range2130000
display id: range2130000
version: 1
direction: direction/forward
top level: SgsE
start: 2401
end: 2406
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2129996
display id: annotation2129996
version: 1
top level: SgsE
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129996/range2129996/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129996/range2129996/1
type: Range
persistent identity: range2129996
display id: range2129996
version: 1
direction: direction/forward
top level: SgsE
start: 73
end: 2409
orientation: inline

BBa_J34814
type: ComponentDefinition
persistent identity: BBa J34814
display id: BBa_J34814
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be used qith t7 polymerase BBa_J34811
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34814_sequence/1

BBa_K113012
type: ComponentDefinition
persistent identity: BBa K113012
display id: BBa_K113012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weaken overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping in two base pairs while a point mutation occurs at the 12th base pair, from A-T to T-A.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113012_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989400
display id: annotation1989400
version: 1
top level: BBa_K113012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113012/annotation1989400/range1989400/1

https://synbiohub.org/public/igem/BBa_K113012/annotation1989400/range1989400/1
type: Range
persistent identity: range1989400
display id: range1989400
version: 1
direction: direction/reverse
top level: BBa_K113012
start: 4
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989402
display id: annotation1989402
version: 1
top level: BBa_K113012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113012/annotation1989402/range1989402/1

https://synbiohub.org/public/igem/BBa_K113012/annotation1989402/range1989402/1
type: Range
persistent identity: range1989402
display id: range1989402
version: 1
direction: direction/forward
top level: BBa_K113012
start: 20
end: 37
orientation: inline

BBa_K525007
type: ComponentDefinition
persistent identity: BBa K525007
display id: BBa_K525007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of S-Layer SgsE with T7/lac and BisdA and BisdB
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PT7 + RBS + SgsE || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525303, BBa_K123000, BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Freiburg Scar
sequence annotation: K525301
sequence annotation: RBS
sequence annotation: BisdB
sequence annotation: B0034
sequence annotation: T7
sequence annotation: ATG
sequence annotation: K525515
sequence annotation: NgoMIV
sequence annotation: AgeI
sequence annotation: lac operator
sequence annotation: BisdA
sequence annotation: SgsE domain
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K525007_sequence/1

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161170
display id: annotation2161170
version: 1
top level: BBa_K525007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161170/range2161170/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161170/range2161170/1
type: Range
persistent identity: range2161170
display id: range2161170
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2401
end: 2406
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2161168
display id: annotation2161168
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161168/range2161168/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161168/range2161168/1
type: Range
persistent identity: range2161168
display id: range2161168
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 2400
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161165
display id: annotation2161165
version: 1
top level: BBa_K525007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161165/range2161165/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161165/range2161165/1
type: Range
persistent identity: range2161165
display id: range2161165
version: 1
direction: direction/forward
top level: BBa_K525007
start: 56
end: 59
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2161174
display id: annotation2161174
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161174/range2161174/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161174/range2161174/1
type: Range
persistent identity: range2161174
display id: range2161174
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2725
end: 3999
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161164
display id: annotation2161164
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161164/range2161164/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161164/range2161164/1
type: Range
persistent identity: range2161164
display id: range2161164
version: 1
direction: direction/forward
top level: BBa_K525007
start: 53
end: 64
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2161162
display id: annotation2161162
version: 1
top level: BBa_K525007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161162/range2161162/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161162/range2161162/1
type: Range
persistent identity: range2161162
display id: range2161162
version: 1
direction: direction/forward
top level: BBa_K525007
start: 1
end: 20
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161166
display id: annotation2161166
version: 1
top level: BBa_K525007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161166/range2161166/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161166/range2161166/1
type: Range
persistent identity: range2161166
display id: range2161166
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 75
orientation: inline

K525515
type: SequenceAnnotation
persistent identity: annotation2161171
display id: annotation2161171
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161171/range2161171/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161171/range2161171/1
type: Range
persistent identity: range2161171
display id: range2161171
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2407
end: 3999
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161169
display id: annotation2161169
version: 1
top level: BBa_K525007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161169/range2161169/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161169/range2161169/1
type: Range
persistent identity: range2161169
display id: range2161169
version: 1
direction: direction/forward
top level: BBa_K525007
start: 76
end: 82
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161175
display id: annotation2161175
version: 1
top level: BBa_K525007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161175/range2161175/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161175/range2161175/1
type: Range
persistent identity: range2161175
display id: range2161175
version: 1
direction: direction/forward
top level: BBa_K525007
start: 3991
end: 3996
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161173
display id: annotation2161173
version: 1
top level: BBa_K525007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161173/range2161173/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161173/range2161173/1
type: Range
persistent identity: range2161173
display id: range2161173
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2719
end: 2724
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2161163
display id: annotation2161163
version: 1
top level: BBa_K525007
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161163/range2161163/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161163/range2161163/1
type: Range
persistent identity: range2161163
display id: range2161163
version: 1
direction: direction/forward
top level: BBa_K525007
start: 20
end: 44
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161172
display id: annotation2161172
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161172/range2161172/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161172/range2161172/1
type: Range
persistent identity: range2161172
display id: range2161172
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2407
end: 2718
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2161167
display id: annotation2161167
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161167/range2161167/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161167/range2161167/1
type: Range
persistent identity: range2161167
display id: range2161167
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 2400
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161176
display id: annotation2161176
version: 1
top level: BBa_K525007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161176/range2161176/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161176/range2161176/1
type: Range
persistent identity: range2161176
display id: range2161176
version: 1
direction: direction/forward
top level: BBa_K525007
start: 3997
end: 3999
orientation: inline

cspB
type: ComponentDefinition
persistent identity: BBa K525224
display id: BBa_K525224
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: S-layer cspB from Corynebacterium halotolerans with PT7 and RBS
mutable notes: PCR
mutable provenance: Corynebacterium halotolerans
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Promoter
sequence annotation: cspB
sequence annotation: B0034
sequence annotation: RBS
sequence annotation: TAT-sequence
sequence: https://synbiohub.org/public/igem/BBa_K525224_sequence/1

Promoter
type: SequenceAnnotation
persistent identity: annotation2184438
display id: annotation2184438
version: 1
top level: cspB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525224/annotation2184438/range2184438/1

https://synbiohub.org/public/igem/BBa_K525224/annotation2184438/range2184438/1
type: Range
persistent identity: range2184438
display id: range2184438
version: 1
direction: direction/forward
top level: cspB
start: 1
end: 22
orientation: inline

cspB
type: SequenceAnnotation
persistent identity: annotation2184442
display id: annotation2184442
version: 1
top level: cspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525224/annotation2184442/range2184442/1

https://synbiohub.org/public/igem/BBa_K525224/annotation2184442/range2184442/1
type: Range
persistent identity: range2184442
display id: range2184442
version: 1
direction: direction/forward
top level: cspB
start: 125
end: 1504
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2184439
display id: annotation2184439
version: 1
top level: cspB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525224/annotation2184439/range2184439/1

https://synbiohub.org/public/igem/BBa_K525224/annotation2184439/range2184439/1
type: Range
persistent identity: range2184439
display id: range2184439
version: 1
direction: direction/forward
top level: cspB
start: 23
end: 34
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2184440
display id: annotation2184440
version: 1
top level: cspB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525224/annotation2184440/range2184440/1

https://synbiohub.org/public/igem/BBa_K525224/annotation2184440/range2184440/1
type: Range
persistent identity: range2184440
display id: range2184440
version: 1
direction: direction/forward
top level: cspB
start: 26
end: 29
orientation: inline

TAT-sequence
type: SequenceAnnotation
persistent identity: annotation2184441
display id: annotation2184441
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525224/annotation2184441/range2184441/1

https://synbiohub.org/public/igem/BBa_K525224/annotation2184441/range2184441/1
type: Range
persistent identity: range2184441
display id: range2184441
version: 1
direction: direction/forward
top level: cspB
start: 34
end: 124
orientation: inline

cspB
type: ComponentDefinition
persistent identity: BBa K525223
display id: BBa_K525223
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-layer cspB from Corynebacterium halotolerans with lipid anchor, PT7 and RBS
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: S-layer cspB from Corynebacterium halotolerans with TAT-Sequence and lipid anchor, PT7 and RBS
mutable notes: PCR
mutable provenance: Corynebacterium halotolerans
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: lipid anchor
sequence annotation: Promoter
sequence annotation: S-layer
sequence: https://synbiohub.org/public/igem/BBa_K525223_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2128113
display id: annotation2128113
version: 1
top level: cspB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525223/annotation2128113/range2128113/1

https://synbiohub.org/public/igem/BBa_K525223/annotation2128113/range2128113/1
type: Range
persistent identity: range2128113
display id: range2128113
version: 1
direction: direction/forward
top level: cspB
start: 21
end: 32
orientation: inline

lipid anchor
type: SequenceAnnotation
persistent identity: annotation2127318
display id: annotation2127318
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525223/annotation2127318/range2127318/1

https://synbiohub.org/public/igem/BBa_K525223/annotation2127318/range2127318/1
type: Range
persistent identity: range2127318
display id: range2127318
version: 1
direction: direction/forward
top level: cspB
start: 1413
end: 1493
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2128114
display id: annotation2128114
version: 1
top level: cspB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525223/annotation2128114/range2128114/1

https://synbiohub.org/public/igem/BBa_K525223/annotation2128114/range2128114/1
type: Range
persistent identity: range2128114
display id: range2128114
version: 1
direction: direction/forward
top level: cspB
start: 1
end: 20
orientation: inline

S-layer
type: SequenceAnnotation
persistent identity: annotation2128115
display id: annotation2128115
version: 1
top level: cspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525223/annotation2128115/range2128115/1

https://synbiohub.org/public/igem/BBa_K525223/annotation2128115/range2128115/1
type: Range
persistent identity: range2128115
display id: range2128115
version: 1
direction: direction/forward
top level: cspB
start: 33
end: 1412
orientation: inline

BBa_K525009
type: ComponentDefinition
persistent identity: BBa K525009
display id: BBa_K525009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and LigA with His-Tag
creator: Manuel Wittchen
created: 2011-10-24T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8718
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and LigA with His-Tag
mutable notes: This part was assembled by Freiburg Standard Assembly (RFC 25)
mutable provenance: SgsE was synthesized, LigA with His-Tag created by PCR. LigA is the natural ligase A of E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K525740
sequence annotation: ATG
sequence annotation: B0034
sequence annotation: Freiburg scar
sequence annotation: T7 promotor
sequence annotation: lac operator
sequence annotation: SgsE domain
sequence annotation: NogMIV
sequence annotation: K525303
sequence annotation: His-Tag
sequence annotation: LigA domain
sequence: https://synbiohub.org/public/igem/BBa_K525009_sequence/1

K525740
type: SequenceAnnotation
persistent identity: annotation2160516
display id: annotation2160516
version: 1
top level: BBa_K525009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160516/range2160516/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160516/range2160516/1
type: Range
persistent identity: range2160516
display id: range2160516
version: 1
direction: direction/forward
top level: BBa_K525009
start: 2410
end: 4434
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160512
display id: annotation2160512
version: 1
top level: BBa_K525009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160512/range2160512/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160512/range2160512/1
type: Range
persistent identity: range2160512
display id: range2160512
version: 1
direction: direction/forward
top level: BBa_K525009
start: 73
end: 75
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160511
display id: annotation2160511
version: 1
top level: BBa_K525009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160511/range2160511/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160511/range2160511/1
type: Range
persistent identity: range2160511
display id: range2160511
version: 1
direction: direction/forward
top level: BBa_K525009
start: 53
end: 64
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160515
display id: annotation2160515
version: 1
top level: BBa_K525009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160515/range2160515/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160515/range2160515/1
type: Range
persistent identity: range2160515
display id: range2160515
version: 1
direction: direction/forward
top level: BBa_K525009
start: 2401
end: 2407
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2160508
display id: annotation2160508
version: 1
top level: BBa_K525009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160508/range2160508/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160508/range2160508/1
type: Range
persistent identity: range2160508
display id: range2160508
version: 1
direction: direction/forward
top level: BBa_K525009
start: 1
end: 20
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160510
display id: annotation2160510
version: 1
top level: BBa_K525009
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160510/range2160510/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160510/range2160510/1
type: Range
persistent identity: range2160510
display id: range2160510
version: 1
direction: direction/forward
top level: BBa_K525009
start: 20
end: 44
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2160513
display id: annotation2160513
version: 1
top level: BBa_K525009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160513/range2160513/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160513/range2160513/1
type: Range
persistent identity: range2160513
display id: range2160513
version: 1
direction: direction/forward
top level: BBa_K525009
start: 73
end: 2409
orientation: inline

NogMIV
type: SequenceAnnotation
persistent identity: annotation2160514
display id: annotation2160514
version: 1
top level: BBa_K525009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160514/range2160514/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160514/range2160514/1
type: Range
persistent identity: range2160514
display id: range2160514
version: 1
direction: direction/forward
top level: BBa_K525009
start: 76
end: 82
orientation: inline

K525303
type: SequenceAnnotation
persistent identity: annotation2160509
display id: annotation2160509
version: 1
top level: BBa_K525009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160509/range2160509/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160509/range2160509/1
type: Range
persistent identity: range2160509
display id: range2160509
version: 1
direction: direction/forward
top level: BBa_K525009
start: 1
end: 2409
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2160518
display id: annotation2160518
version: 1
top level: BBa_K525009
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160518/range2160518/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160518/range2160518/1
type: Range
persistent identity: range2160518
display id: range2160518
version: 1
direction: direction/forward
top level: BBa_K525009
start: 4417
end: 4434
orientation: inline

LigA domain
type: SequenceAnnotation
persistent identity: annotation2160517
display id: annotation2160517
version: 1
top level: BBa_K525009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160517/range2160517/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160517/range2160517/1
type: Range
persistent identity: range2160517
display id: range2160517
version: 1
direction: direction/forward
top level: BBa_K525009
start: 2407
end: 4416
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Consensus Promoter Sequence
creator: Barry Canton
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
direction: direction/forward
top level: BBa_R0085
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
direction: direction/forward
top level: BBa_R0085
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
direction: direction/forward
top level: BBa_R0085
start: 18
end: 18
orientation: inline

LigA
type: ComponentDefinition
persistent identity: BBa K525710
display id: BBa_K525710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: LigA1 mit Promotor, RBS, Histag
mutable notes: PCR
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: B0034
sequence annotation: ATG
sequence annotation: His-tag
sequence annotation: LigA
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K525710_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2161301
display id: annotation2161301
version: 1
top level: LigA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161301/range2161301/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161301/range2161301/1
type: Range
persistent identity: range2161301
display id: range2161301
version: 1
direction: direction/forward
top level: LigA
start: 24
end: 27
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161300
display id: annotation2161300
version: 1
top level: LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161300/range2161300/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161300/range2161300/1
type: Range
persistent identity: range2161300
display id: range2161300
version: 1
direction: direction/forward
top level: LigA
start: 21
end: 33
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161302
display id: annotation2161302
version: 1
top level: LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161302/range2161302/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161302/range2161302/1
type: Range
persistent identity: range2161302
display id: range2161302
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 36
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2161304
display id: annotation2161304
version: 1
top level: LigA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161304/range2161304/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161304/range2161304/1
type: Range
persistent identity: range2161304
display id: range2161304
version: 1
direction: direction/forward
top level: LigA
start: 2047
end: 2064
orientation: inline

LigA
type: SequenceAnnotation
persistent identity: annotation2161303
display id: annotation2161303
version: 1
top level: LigA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161303/range2161303/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161303/range2161303/1
type: Range
persistent identity: range2161303
display id: range2161303
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 2045
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2161299
display id: annotation2161299
version: 1
top level: LigA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161299/range2161299/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161299/range2161299/1
type: Range
persistent identity: range2161299
display id: range2161299
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 20
orientation: inline

pT7
type: ComponentDefinition
persistent identity: BBa K525998
display id: BBa_K525998
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter T7 and RBS
creator: Anna Drong
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter T7 and RBS
mutable notes: Synthesis
mutable provenance: Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: B0034
sequence annotation: T7 promoter
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K525998_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2127788
display id: annotation2127788
version: 1
top level: pT7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2127788/range2127788/1

https://synbiohub.org/public/igem/BBa_K525998/annotation2127788/range2127788/1
type: Range
persistent identity: range2127788
display id: range2127788
version: 1
direction: direction/forward
top level: pT7
start: 21
end: 32
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2129432
display id: annotation2129432
version: 1
top level: pT7
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2129432/range2129432/1

https://synbiohub.org/public/igem/BBa_K525998/annotation2129432/range2129432/1
type: Range
persistent identity: range2129432
display id: range2129432
version: 1
direction: direction/forward
top level: pT7
start: 1
end: 20
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129433
display id: annotation2129433
version: 1
top level: pT7
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2129433/range2129433/1

https://synbiohub.org/public/igem/BBa_K525998/annotation2129433/range2129433/1
type: Range
persistent identity: range2129433
display id: range2129433
version: 1
direction: direction/forward
top level: pT7
start: 24
end: 27
orientation: inline