Terminator of Yeast mitochondrial gene COX3
This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region. http://www.yeastgenome.org/
This is the terminator region for COX3 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
We modelled the RNA secondary structure of COX3 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
The CpxR comes from the genome of E coli, and the arabinose promoter comes from a biobrick.
The part contains CpxR with an upstream arabinose-inducible promoter.
The arabinose had to be upstream of the CpxR. Also, the sequence had to be tested because of a mutation, and it works perfectly.
Two terminators in Bgl operon that is antiterminated by bglG
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.
trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
The part has been extracted from the E coli genome.
CpxR is a transciption factor that dimerizes and becomes active when phosphorylated by the transmembrane histidine kinase CpxA. CpxA in turn becomes active when the bacterial cell is submitted to different kinds of shock (pH, temperature, pressure, protein misfolding in the inter-membrane space...).
When making composite parts with CpxR that are supposed to dimerize, we had to take care about the positioning of the other elements regarding the parallel dimerization of CpxR.
sgRNA with PAM-Proximal complementary sequence 4
Modification of biobricks part BBa_B0015
T7 gene 1 specific transcriptional terminator
eukaryotic -- derived from SV40 early poly A signal sequence
It is cloned in BioBrick vector pSB1AK3.
Reverse Bi-directional terminator from B0050 (derived from pBR322).
This part is the reverse of terminator B0013, a bacteriophage T7 terminator which was engineered to be bidirectional. Contains a poly A insertion upstream of a 20 bp stem-loop.
Double terminator consisting of BBa_B0012 and BBa_B0011
This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.
This is the B0010 part extracted by PCR from the doulbe terminator B0012. We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use! The characterization has been done both with T7 and Coli RNApolymerases.
I don't have any design considerations, anyway it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.
Blue Fluorescent Protein mTagBFP generator (Strong RBS)
Improved version of BBa_K592024 by adding a terminator BBa_B1006 3??? to this part.
The double terminator was already registered in the iGEM catalogue. The zwf promotor comes from the genome of Pseudomonas putida. The mCherry is codon optimized for expression in Pseudomonas.
This device acts as a detector for glucose (and certain derivatives of it). The double transcription terminator ensures, that only the gene downstream of the promotor zwf is influenced by it. The zwf promotor (BBa_K1840002) is contained in the genome of Pseudomonas putida and is situated in front of the enzyme zwf (glucose 6-phosphate dehydrogenase). The repressor HexR can bind the zwf promotor sequence and thus prevent the transcription of the zwf gene. In case the glucose derivative 2-Keto-3-deoxy-6-phosphogluconate is present, it binds to the promotor and leads to a subsequent conformational change. Hence, the repressor can no longer bind and the gene(s) downstream can be transcribed. In our device, the downstream gene is mCherry, codon optimized for Pseudomonas (BBa_K1840004). Therefore, in the presence of glucose, mCherry is expressed. In various experiments, we could see that this device is in deed working.
As promotor sequence, we assumed all DNA in front of the zwf gene up to the next gene, coding for the repressor HexR. Since it is encoded on the opposite strand, there is the possibility, that zwf functions as a promotor for this gene as well. Therefore the double terminator was needed.
Artificial terminator, estimated %T~=25% * 6bp stem, 4nt loop * Bidirectional, estimated reverse %T~<10%
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 3 residues. Designed to be a terminator with low efficiency.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 V01146 .
Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream. Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
BGH poly A, terminator for use in mammalian cells
Compatible with the Plug'n'Play assembly standard
Bi-directional Terminator from pSMART HCKAN. E.coli derived, genbank U24206 bases 2694-2727 between yciA and tonA genes (converging transcripts)
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Composite part fot TetLVA TetPromoter and T4
This part is a composite structure which contains a promoter, RBS side and and a tetracycline repressor from transposon Tn10. It is transcribed in the opposite direction for the coding sequence of the repressor AraC. Through the binding to L+arabinose, the conformation of AraC changes.This causes the protein to diffuses from the DNA thereby inducing transcription. Through the RBS, the definition of the RBS efficiency could be observed. As the second part we added Terminator+Tetr Promoter+T4 Endolysin. For this part an artifical Terminator is used (generally added to another parts). The promoter is a TetR repressible promoter.It is sequenced for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog part
L(+) - Arabinose is in sugar form and harmless The designed promoter has low background activity, so the membrane proteins will not damage the cells before induction. Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues. High expression of lysozyme only is toxic to bacteria. BBa_R0040 (TetR repressible promoter) is based on a cI promoter. It has been modified to include two TetR binding sites. Constructed by DNA synthesis.
References: //parts.igem Schleif, R. (2000). "Regulation of the L-arabinose operon of Escherichia coli." Trends Genet 16(12): 559-565. Ren, H., D. Yu, et al. (2009). "High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1 http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare http://www.ncbi.nlm.nih.gov/pubmed/7768852?dopt=Abstract Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs BiblioPlus Extension Error fetching PMID 9092630:
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtg aaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatgg tgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttc aaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaat tagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattac ctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg atgaactatacaaataataa ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc accttcgggtgggcctttctgcgtttata
The iGEM Team Panama 2011 have to design and assemble the ends of an expression platform in order to bring up by rational designing a fuctional test platform for the expression of our Team favorite BioBrick part BBa_K653000 A.K. The Biosurfactator. We choose to assembly two existing BioBrick parts from the shop, we mean The Registry. Those parts are: a GFP reporter BBa_E0040 and a double terminator BBa_B0015. We assemble them to bring up a new composite part that will serve as a well characterized ends for a expression platform for several coding sequences like our RhlAB_BBa_K653000
This GFP part was derived from the reporter device used by Elowitz's repressilator : GFPmut3* has a couple of mutations from wtGFP - S2R and S65G and S72A. Emerald and EGFP have S65T and multiple other mutations. The S2R mutation should have no effect. The Double terminator consisting of BBa_B0010 and BBa_B0012. This is the most commonly used terminator. It seems to be reliable. The assembling of this two parts was performed using the 3A Assembly Method.
Artificial terminator, estimated %T~=55 * 6bp stem, 4nt loop * Bidirectional, estimated reverse %T~<20
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 4 residues.
Reverse of bi-directional terminator B0051. (E.coli derived, genbank U24206 bases 2694-2727. between converging yciA and tonA genes.)
NosT plant specific terminator w/ Stop codon
Amplified via PCR from pORE vector E3, TGA stop codon added on 5' end
Derived from the pORE series of vectors (see http://2010.igem.org/Team:Harvard)
GFP originates from jellyfish Aequeora victoria. For more details visit the [http://www.expasy.ch/uniprot/P42212 SwissProt entry]. The double terminator was designed by Reshma Shetty. For more details visis the [http://partsregistry.org/Part:BBa_B0014 original part page].
==Part Description== This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator. Green Fluorescent protein (originally from part [http://partsregistry.org/Part:BBa_E0040 BBa_E0040]) that will glow green when excited by the correct wavelengths and thus can be used as a reporter gene. This part is directly fused to a terminator (originally from part [http://partsregistry.org/Part:BBa_B0014 BBa_B0014]) so it is ideal to be fused at the end of your construct. ==Table of Statistics== {| style="color:white; background-color:#0066CC;" cellpadding="20" cellspacing="0" border="2" !Chassis !Monomer/Multimer !Aminoacid Length/Weight !Localisation !Structure !Excitation wavelengths !Emmision wavelengths |- |Prokaryotic and Eukaryotic |Bipartite |274 aminoacids | |beta-barrel(for GFP) hairpins for Terminator |501nm |511nm |} ==References== #Valdivia pmid=8707053
No additional design considerations. The parts were fused together following RFC10 assembly.
SV40 poly A, terminator for mammalian cells
SV40 poly A, terminator for use in mammalian cells
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
T2-motni, terminator for Aspergillus niger
T2-motni is a terminator for use in Aspergillus niger
For this part an artifical Terminator is used (generally added to another parts). The promoter is a TetR repressible promoter.It is sequenced for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog part
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues. High expression of lysozyme only is toxic to bacteria. BBa_R0040 (TetR repressible promoter) is based on a cI promoter. It has been modified to include two TetR binding sites. Constructed by DNA synthesis.
Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs BiblioPlus Extension Error fetching PMID 9092630:
CYC1 Terminator is widely used in Saccharomyces cerevisiae. It is a composite part in many vectors of Saccharomyces cerevisiae like plasmid pYES2. Amplifying it by PCR and link it after your protein coding sequence is also feasible if you want to express the gene in Saccharomyces cerevisiae.
There is no neccessary consideration in detailed design of the sequence
It comes from genome sequence of Saccharomyces cerevisiae
Variant of GFP that folds robustly even when fused to poorly folded proteins. Waldo and other research groups has reported the engineering of a superfolder GFP (sfGFP) that showed increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding (Pedelacq et al., 2006; Andrews et al., 2007; Fisher and DeLisa, 2008). sfGFP has proven to be very useful as a scaffold for improved protein detection and tagging both in vivo and in vitro using self-assembled sfGFP fragments (Cabantous et al., 2005b; Cabantous and Waldo, 2006). Furthermore, sfGFP fusions are more soluble than conventional GFP fusions (Wu et al., 2009).
two start codons coding for RFP and sfGFP reporters
This part contains no direct BioBrick parts. It was assembled from several pieces of plasmid DNA in the Salis Lab at Penn State. The dRBS1 vector and pBAC reporter components were assembled from PCR-amplified fragments.
This part was used in a design that tests multiple start codons. The first start codon codes for RFP. The second start codon codes for sfGFP. They are 7 nucleotides apart on the DNA strand. The ribosome binding site translation initiation rate determines which protein is expressed. The part has two restriction enzyme sites flanking the RBS and promoter regions, so varying RBS strengths may be tested.
This design used gBlocks synthesized by IDT. Several CBAR reactions were necessary to achieve the construct.
Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
Artificial terminator, estimated %T~=80% # 8bp stem, 6nt loop # Bidirectional, estimated reverse %T~>90%
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 6 residues.
Bidirectional transcriptional terminator cloned in the reverse direction of B0011. 22 bp stem-loop
The part was designed after information from the paper by DADDAOUA, A., KRELL, T. & RAMOS, J.-L. 2009. Regulation of Glucose Metabolism in Pseudomonas: The Phosphorylative Branch and Entner-Doudoroff Enzymes are Regulated by a Repressor Containing a Sugar Iisomerase Domain. Journal of Biological Chemistry, 284, 21360-21368.
This basic part is the promotor in front of the gene for zwf (glucose 6-phosphate dehydrogenase) in Pseudomonas putida. Zwf is concerned with the glucose metabolism in P. putida. The zwf promotor is negatively regulated by the repressor called HexR. It has a binding site for glucose derivative 2-keto-3-deoxy-6-phosphogluconate (KDPG) and binding leads to the release from the promotor. For our project (NTNU Trondheim 2015) we integrated the promotor in front of the mCherry gene. Hence we built a glucose detection device, that expresses mCherry according to the glucose (and therefore KDPG) level in the environment. Note: The promotor edd (BBa_K1840000) is repressed by the same repressor HexR. See biobrick parts BBa_K1840001 and BBa_K1840003 for other promotors on operons regulated by glucose derivatives.
The considerations we made regarded the starting and endpoint of the promotor. We only know that it has to be in front of the zwf gene. We assumed it starts directly after the previous gene. This gene codes for the repressor, HexR, and is situated on the opposite strand.
Double terminator with two copies of <bb_part>BBa_B0010</bb_part>
Terminator from the E.coli ribosonal RNA rrnC operon. This terminator occurs in the reverse orientation upstream of the multiple cloning site of the pSB**1-pSB**3 series of plasmids.
Plant type transcriptional terminator NosT.
hGH poly A, terminator for mammalian cells
From plasmid N-terminal pFLAG-CMV from Sigma-Aldrich
Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
identical to E. coli MG1655 between tonB and yciA
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
T1-motni, terminator for Aspergillus niger
T1-motni is terminator that works in the fungus Aspergillus niger
The terminator is derived from part b1006. pAB is entered as part K187000.
This part contains a bidirectional, high efficiency terminator, in the BioBytes universal plasmid pAB.See the BioBytes RFC for details on the BioBytes assembly method.
A terminator with long sticky ends can be prepared from this plasmid and assembled downstream of a gene using the Biobytes method. The addition of one DNA segment with the BioBytes method takes only 20 minutes. This ability to easily 'mix and match' terminators and genes allows rapid testing of different terminator designs.
35S Terminator of Cauliflower Mosaic Virus (CaMV)
This part contains the t35S terminator of the Cauliflower Mosaic Virus (CaMV), a plant virus. This part is flanked by RFC[10] pre- and suffix.
This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
Flexible linker used to fuse two proteins.
Designed considering the flexibility and separation needed between our fused proteins.
This is the second part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[1].
When fused to another coding sequence, insert a flexible linker.
It comes from the standard mCherry sequence and was condon optimized.
The standard fluorescent protein mCherry is not efficiently expressed in Pseudomonas spp. Therefore this biobrick part is a codon optimized mCherry for the expression in Pseudomonas. We used it in our project (NTNU Trondheim 2015) in a device as reporter. Note: See biobrick parts BBa_K1840005 to BBa_K1840008 for the characterization of the devices.
The expression mechanism in Pseudomonas had to be considered to be able to codon optimize it.
The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).
We had to test both attachments, N and C terminus of the CpxR.
double terminator (B0012-B0011), reversed
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
GFP (mut3b) [note that this part does not have a barcode]
Artificial terminator, estimated %T~<10% 8bp stem, 6nt loop Bidirectional, estimated reverse %T~=40%
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 3 residues.
T3-motni, terminator for Aspergillus niger
T3-motni is a terminator for use in Aspergillus niger.
Artificial terminator, estimated %T~>90% * 8bp stem, 6nt loop * Bidirectional, estimated reverse %T~>90%
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 4 residues
Artifical terminator, estimated %T~=80 6bp stem, 4nt loop Bidirectional, estimated reverse %T=40
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 5 residues.
Bi-directional Terminator derived from pBR322.
Reverse of B0053, E.coli transcriptional terminator from the His operon.
Terminator of gyrase (EC 5.99.1.3) gene.
Our terminator is based on strong, Rho-independent terminator of gyrase gene, which is type II topoisomerase (EC 5.99.1.3). DNA gyrase uses the energy of ATP hydrolysis to introduce negative supercoils or relaxes positive supercoils. Negative DNA supercoiling is essential in vivo to compact the genome, relieve torsional strain during replication, and promote local melting for vital processes such as transcript initation by RNA polymerase. Gyrase gene is an housekeeping gene and because of that it must have efficient terminator.
Device expressing the Gb3 Synthase enzyme under control of a constitutive promoter
A constitutive promoter was added to the sequence
Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006 This version was synthesised de novo (by Geneart).
This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). It carries the following amino acid changes with respect to mut3 GFP (E0040), the currently most commonly used GFP in the registry: S30R, Y39N, F64L, G65T, F99S, N105T, Y145F, M153T, V163A, I171V, A206V Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP. Note: Superfolder GFP is available in constructs driven by the pBAD and T7 promoters: part numbers I746908 and I746909 respectively. Additionally 6-his tagged versions for protein purification exist: I746914 (pBAD driven) and I746915 (T7 driven).
Codon optimisation before de novo synthesis was carried out for both, E.coli and Bacillus subtilis.
This is an efficient termintor sequence from Bacillus that normally terminates the rrnO-16S-trnO-Ile-trnO-Ala-rrnO-23S-rrnO-5S operon.
This terminator forms part of the complex device BBa_K104001
The sequence was derived from DBTBS http://dbtbs.hgc.jp/COG/prom/rrnO-16S-trnO-Ile-trnO-Ala-rrnO-23S-rrnO-5S.html
Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter
An inducible promoter (BBa_10500), RBS (BBa_B0034) Scar was added to the sequence. Along with a terminator.
This is the exact region between the end of the ORF of STE2-W and the beginning of BST1-C
This sequence was taken from the yeast genome
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pair
Artificial terminator, estimated %T~=70% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~=80%
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 5 residues.
trpC terminator for Aspergillus nidulans
From the plasmid p71 from the Center for Microbial Biotechnology at the Technical University of Denmark plasmid collection.
TtrpC is a terminator of tryptophan synthase of Aspergillus nidulans.
This is the first part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[2].
The T0 transcriptional terminator terminates transcription with 80-90% efficiency.
Double terminator consisting of BBa_B0010 and BBa_B0012
Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak).
Engineered 'cyc100 promoter' of Saccharomyces cerevisiae with Kozak sequence attached downstream.
Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
Antiquity. thought to be T1 transcriptional terminator from E. coli RRNB operon.
Transcriptional terminator consisting of a 64 bp stem-loop (B0010), cloned onto the antisense strand.
Terminator in Bgl operon that is antiterminated by bglG
The lambda t1 is a strong transcriptional terminator with 99% efficiency in vivo.
Strong transcriptional terminator consisting of a 20 bp stem-loop from Salmonella phage P22.
Transcriptional terminator consisting of a 64 bp stem-loop.
Terminator of Yeast mitochondrial gene Q0255
We modelled the RNA secondary structure of Q0255 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
This is the terminator region for Q0255 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
Uni-directional terminator. Reverse of part B0012. 20 bp stem-loop.
Blue Fluorescent Protein mTagBFP generator (Medium RBS)
Improved version of BBa_K592023 by adding a terminator BBa_B1006 3???to the part.
sgRNA with PAM-Proximal complementary sequence 6
Derived from luxICDABEG operon terminator of Vibrio fischeri AF170104 .
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
* taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
Conversion of the iGEM parts registry to SBOL2.1