//terminator/double
type: Collection
persistent identity: terminator double
display id: terminator_double
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: Zwf-Device
member: BBa_K1031224
member: BBa_K1031422
member: BBa_K1031620
member: BBa_C0450
member: BBa_K1031100
member: BBa_K1031410
member: BBa_K1486025
member: BBa_K1031625
member: BBa_K1031221
member: BBa_K2122100
member: BBa_K1031622
member: BBa_K1031923
member: BBa_K1031114
member: BBa_K1031510
member: BBa_B0014
member: BBa_K1031941
member: BBa_K1031804
member: BBa_K2122000
member: Reporter +
member: BBa_K1031623
member: BBa_K933000
member: BBa_K1031111
member: BBa_K1031624
member: BBa_K1031520
member: BBa_K1031913
member: BBa_B0025
member: BBa_K1031112
member: BBa_K1031302
member: BBa_K1031521
member: BBa_K1486005
member: BBa_B0017
member: BBa_K1031931
member: BBa_K1031621
member: BBa_B0024
member: BBa_K1031113
member: BBa_K1031311
member: BBa_K1031922
member: BBa_K1031803
member: BBa_B0015
member: BBa_K259006
member: BBa_I11013
member: BBa_K1031222
member: BBa_K1031420
member: BBa_K1031916
member: BBa_K1031223
member: BBa_K1031421
member: BBa_K1486001
member: BBa_K1031301
member: BBa_K1031917
member: BBa_K1486009
member: BBa_K1031710

//terminator/single
type: Collection
persistent identity: terminator single
display id: terminator_single
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K801012
member: BBa_J61048
member: BBa_B0020
member: BBa_K731722
member: BBa_B1002
member: BBa_B1007
member: BBa_B0016
member: BBa_I51003
member: tADH1
member: BBa_B0011
member: BBa_B0050
member: BBa_K678012
member: BBa_K864600
member: trkD
member: BBa_B1005
member: BBa_B0062
member: BBa_B0053
member: BGH pA
member: BBa_B0023
member: TtrpC
member: BBa_B0060
member: BBa_C0440
member: BBa_K404108
member: BBa_B0013
member: Q0255 term
member: BBa_B0055
member: BBa_K780000
member: BBa_B0012
member: BBa_B1010
member: BBa_Y1015
member: BBa_K678039
member: BBa_B1006
member: BBa_B0063
member: BBa_B1001
member: COX3 termi
member: BBa_B0054
member: hGH pA
member: T22
member: BBa_B0061
member: BBa_B1008
member: BBa_K110012
member: BBa_K801011
member: BBa_B0010
member: BBa_K187025
member: T7stop
member: BBa_B1009
member: BBa_K864601
member: BBa_B1004
member: BBa_K404116
member: BBa_K678037
member: BBa_B0022
member: BBa_B0052
member: BBa_B0021
member: BBa_B1003
member: BBa_J52016
member: BBa_K678038
member: BBa_B0051
member: BBa_K392003

//terminator
type: Collection
persistent identity: terminator
display id: terminator
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K1468001
member: //terminator/single
member: BBa_K382023
member: MaTif
member: BBa_K1484215
member: BBa_K1994045
member: BBa_K1899002
member: BBa_K1486025
member: BBa_K1994043
member: //terminator/double
member: BBa_K1899001
member: BBa_K1462070
member: BBa_K2110000
member: BBa_K1392972
member: BBa_K1468002
member: BBa_K1468000
member: BBa_K2042007
member: BBa_K1468004
member: BBa_K382024
member: BBa_K1899003
member: BBa_K1392970
member: BBa_K104007
member: BBa_K1159307

BBa_K2122100
type: ComponentDefinition
persistent identity: BBa K2122100
display id: BBa_K2122100
version: 1
was generated by: iGEM to SBOL conversion
description: Device expressing the Gb3 Synthase enzyme under control of a constitutive promoter
created: 2016-09-28T11:00:00Z
modified: 2016-10-18T01:30:37Z
mutable provenance: Coding Sequence from GenBank
owned by: james
owned by: myers
mutable description: This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
discontinued: false
dominant: false
experience: experience/None
group u list: _2591_
m user id: 32074
owner id: 32074
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: A constitutive promoter was added to the sequence
star: false
creator: Sannia Farrukh
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23100
component: BBa_B0034
component: BBa_B0014
component: BBa_K2122150
sequence annotation: BBa_J23100
sequence annotation: BBa_B0014
sequence annotation: BBa_B0034
sequence annotation: BBa_K2122150
sequence: https://synbiohub.org/public/igem/BBa_K2122100_sequence/1

BBa_J23100
type: Component
persistent identity: component2518013
display id: component2518013
version: 1
top level: BBa_K2122100
access: public
definition: BBa_J23100

BBa_B0034
type: Component
persistent identity: component2518015
display id: component2518015
version: 1
top level: BBa_K2122100
access: public
definition: BBa_B0034

BBa_B0014
type: Component
persistent identity: component2518023
display id: component2518023
version: 1
top level: BBa_K2122100
access: public
definition: BBa_B0014

BBa_K2122150
type: Component
persistent identity: component2518016
display id: component2518016
version: 1
top level: BBa_K2122100
access: public
definition: BBa_K2122150

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2518013
display id: annotation2518013
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518013/range2518013/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518013/range2518013/1
type: Range
persistent identity: range2518013
display id: range2518013
version: 1
top level: BBa_K2122100
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2518023
display id: annotation2518023
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518023/range2518023/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518023/range2518023/1
type: Range
persistent identity: range2518023
display id: range2518023
version: 1
top level: BBa_K2122100
direction: direction/reverse
start: 1132
end: 1226
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518015
display id: annotation2518015
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518015/range2518015/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518015/range2518015/1
type: Range
persistent identity: range2518015
display id: range2518015
version: 1
top level: BBa_K2122100
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K2122150
type: SequenceAnnotation
persistent identity: annotation2518016
display id: annotation2518016
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518016/range2518016/1
component: BBa_K2122150

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518016/range2518016/1
type: Range
persistent identity: range2518016
display id: range2518016
version: 1
top level: BBa_K2122100
direction: direction/reverse
start: 62
end: 1123
orientation: inline

BBa_K1484215
type: ComponentDefinition
persistent identity: BBa K1484215
display id: BBa_K1484215
version: 1
was generated by: iGEM to SBOL conversion
description: nopaline synthase terminator
creator: Salil Bhate
created: 2014-10-07T11:00:00Z
modified: 2015-06-10T02:20:23Z
discontinued: false
dominant: false
group u list: _1864_
m user id: 4206
owner id: 21057
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: MP, Arab use
mutable notes: Cascading for use in MP
mutable provenance: Arab genom
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PlantSyntax Terminator + 3'U
sequence: https://synbiohub.org/public/igem/BBa_K1484215_sequence/1

PlantSyntax Terminator + 3'U
type: SequenceAnnotation
persistent identity: annotation2414249
display id: annotation2414249
version: 1
top level: BBa_K1484215
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1484215/annotation2414249/range2414249/1

https://synbiohub.org/public/igem/BBa_K1484215/annotation2414249/range2414249/1
type: Range
persistent identity: range2414249
display id: range2414249
version: 1
direction: direction/forward
top level: BBa_K1484215
start: 1
end: 11
orientation: inline

PlantSyntax Terminator + 3'U
type: SequenceAnnotation
persistent identity: annotation2414250
display id: annotation2414250
version: 1
top level: BBa_K1484215
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1484215/annotation2414250/range2414250/1

https://synbiohub.org/public/igem/BBa_K1484215/annotation2414250/range2414250/1
type: Range
persistent identity: range2414250
display id: range2414250
version: 1
direction: direction/forward
top level: BBa_K1484215
start: 282
end: 293
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: B1006
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

BBa_B1003
type: ComponentDefinition
persistent identity: BBa B1003
display id: BBa_B1003
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, small, %T~=80)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artifical terminator, estimated %T~=80 6bp stem, 4nt loop Bidirectional, estimated reverse %T=40
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 5 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: stem loop
sequence annotation: B1003
sequence: https://synbiohub.org/public/igem/BBa_B1003_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1898418
display id: annotation1898418
version: 1
top level: BBa_B1003
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898418/range1898418/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898418/range1898418/1
type: Range
persistent identity: range1898418
display id: range1898418
version: 1
top level: BBa_B1003
direction: direction/reverse
start: 5
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898417
display id: annotation1898417
version: 1
top level: BBa_B1003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898417/range1898417/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898417/range1898417/1
type: Range
persistent identity: range1898417
display id: range1898417
version: 1
top level: BBa_B1003
direction: direction/forward
start: 10
end: 25
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898419
display id: annotation1898419
version: 1
top level: BBa_B1003
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898419/range1898419/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898419/range1898419/1
type: Range
persistent identity: range1898419
display id: range1898419
version: 1
top level: BBa_B1003
direction: direction/forward
start: 26
end: 30
orientation: inline

B1003
type: SequenceAnnotation
persistent identity: annotation1898416
display id: annotation1898416
version: 1
top level: BBa_B1003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898416/range1898416/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898416/range1898416/1
type: Range
persistent identity: range1898416
display id: range1898416
version: 1
top level: BBa_B1003
direction: direction/forward
start: 1
end: 34
orientation: inline

tADH1
type: ComponentDefinition
persistent identity: BBa J63002
display id: BBa_J63002
version: 1
was generated by: iGEM to SBOL conversion
description: ADH1 terminator from S. cerevisiae
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: genomic DNA of S. cerevisiae
owned by: james
owned by: myers
mutable description: ADH1 terminator from S. cerevisiae
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: starts with stop codon
star: false
creator: Caroline Ajo-Franklin
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ADH1 terminator
sequence: https://synbiohub.org/public/igem/BBa_J63002_sequence/1

ADH1 terminator
type: SequenceAnnotation
persistent identity: annotation1902851
display id: annotation1902851
version: 1
top level: tADH1
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_J63002/annotation1902851/range1902851/1

https://synbiohub.org/public/igem/BBa_J63002/annotation1902851/range1902851/1
type: Range
persistent identity: range1902851
display id: range1902851
version: 1
top level: tADH1
direction: direction/forward
start: 1
end: 225
orientation: inline

BBa_K1031224
type: ComponentDefinition
persistent identity: BBa K1031224
display id: BBa_K1031224
version: 1
was generated by: iGEM to SBOL conversion
description: Po-B0034-sfGFP-Terminator (DmpR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Po-34-sfGFP-TT (DmpR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0015
sequence annotation: B0034
sequence annotation: po promoter
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031224_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2354393
display id: annotation2354393
version: 1
top level: BBa_K1031224
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354393/range2354393/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354393/range2354393/1
type: Range
persistent identity: range2354393
display id: range2354393
version: 1
top level: BBa_K1031224
direction: direction/forward
start: 929
end: 1057
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2354391
display id: annotation2354391
version: 1
top level: BBa_K1031224
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354391/range2354391/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354391/range2354391/1
type: Range
persistent identity: range2354391
display id: range2354391
version: 1
top level: BBa_K1031224
direction: direction/forward
start: 180
end: 191
orientation: inline

po promoter
type: SequenceAnnotation
persistent identity: annotation2354390
display id: annotation2354390
version: 1
top level: BBa_K1031224
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354390/range2354390/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354390/range2354390/1
type: Range
persistent identity: range2354390
display id: range2354390
version: 1
top level: BBa_K1031224
direction: direction/forward
start: 1
end: 171
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354392
display id: annotation2354392
version: 1
top level: BBa_K1031224
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354392/range2354392/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354392/range2354392/1
type: Range
persistent identity: range2354392
display id: range2354392
version: 1
top level: BBa_K1031224
direction: direction/forward
start: 198
end: 920
orientation: inline

BBa_K1392972
type: ComponentDefinition
persistent identity: BBa K1392972
display id: BBa_K1392972
version: 1
was generated by: iGEM to SBOL conversion
description: Composite part fot TetLVA TetPromoter and T4
creator: Şeniz Y??ksel Burak Kızıl
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1769_
m user id: 0
owner id: 16902
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a composite structure which contains a promoter, RBS side and and a tetracycline repressor from transposon Tn10. It is transcribed in the opposite direction for the coding sequence of the repressor AraC. Through the binding to L+arabinose, the conformation of AraC changes.This causes the protein to diffuses from the DNA thereby inducing transcription. Through the RBS, the definition of the RBS efficiency could be observed. As the second part we added Terminator+Tetr Promoter+T4 Endolysin. For this part an artifical Terminator is used (generally added to another parts). The promoter is a TetR repressible promoter.It is sequenced for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog part
mutable notes: L(+) - Arabinose is in sugar form and harmless The designed promoter has low background activity, so the membrane proteins will not damage the cells before induction. Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues. High expression of lysozyme only is toxic to bacteria. BBa_R0040 (TetR repressible promoter) is based on a cI promoter. It has been modified to include two TetR binding sites. Constructed by DNA synthesis.
mutable provenance: References: //parts.igem Schleif, R. (2000). "Regulation of the L-arabinose operon of Escherichia coli." Trends Genet 16(12): 559-565. Ren, H., D. Yu, et al. (2009). "High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1 http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare http://www.ncbi.nlm.nih.gov/pubmed/7768852?dopt=Abstract Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs BiblioPlus Extension Error fetching PMID 9092630:
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_R0040
component: BBa_K808000
component: BBa_B1006
component: BBa_K112806
component: BBa_C0040
sequence annotation: BBa_B1006
sequence annotation: BBa_C0040
sequence annotation: BBa_K808000
sequence annotation: BBa_K112806
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1392972_sequence/1

BBa_B0034
type: Component
persistent identity: component2415824
display id: component2415824
version: 1
top level: BBa_K1392972
access: public
definition: BBa_B0034

BBa_R0040
type: Component
persistent identity: component2415834
display id: component2415834
version: 1
top level: BBa_K1392972
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K808000
type: Component
persistent identity: component2415822
display id: component2415822
version: 1
top level: BBa_K1392972
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_B1006
type: Component
persistent identity: component2415833
display id: component2415833
version: 1
top level: BBa_K1392972
access: public
definition: BBa_B1006

BBa_K112806
type: Component
persistent identity: component2415840
display id: component2415840
version: 1
top level: BBa_K1392972
access: public
definition: https://synbiohub.org/public/igem/BBa_K112806/1

BBa_C0040
type: Component
persistent identity: component2415828
display id: component2415828
version: 1
top level: BBa_K1392972
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2415833
display id: annotation2415833
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415833/range2415833/1
component: BBa_B1006

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type: Range
persistent identity: range2415833
display id: range2415833
version: 1
direction: direction/reverse
top level: BBa_K1392972
start: 1929
end: 1967
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2415828
display id: annotation2415828
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415828/range2415828/1
component: BBa_C0040

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type: Range
persistent identity: range2415828
display id: range2415828
version: 1
direction: direction/reverse
top level: BBa_K1392972
start: 1236
end: 1920
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2415822
display id: annotation2415822
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415822/range2415822/1
component: BBa_K808000

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type: Range
persistent identity: range2415822
display id: range2415822
version: 1
direction: direction/reverse
top level: BBa_K1392972
start: 1
end: 1209
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2415840
display id: annotation2415840
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415840/range2415840/1
component: BBa_K112806

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type: Range
persistent identity: range2415840
display id: range2415840
version: 1
direction: direction/reverse
top level: BBa_K1392972
start: 2038
end: 2551
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2415824
display id: annotation2415824
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415824/range2415824/1
component: BBa_B0034

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type: Range
persistent identity: range2415824
display id: range2415824
version: 1
direction: direction/reverse
top level: BBa_K1392972
start: 1218
end: 1229
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2415834
display id: annotation2415834
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415834/range2415834/1
component: BBa_R0040

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type: Range
persistent identity: range2415834
display id: range2415834
version: 1
direction: direction/forward
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start: 1976
end: 2029
orientation: inline

BBa_K1468004
type: ComponentDefinition
persistent identity: BBa K1468004
display id: BBa_K1468004
version: 1
was generated by: iGEM to SBOL conversion
description: pTet + gene encoding LacZ
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_K1468004
sequence annotation: C --> T
sequence annotation: -35
sequence annotation: LacZ protein
sequence annotation: TetR 1
sequence annotation: BBa_R0040
sequence annotation: STOP
sequence annotation: BBa_I732005
sequence annotation: START
sequence annotation: TetR 2
sequence annotation: LacZ
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1468004_sequence/1

BBa_K1468004
type: SequenceAnnotation
persistent identity: annotation2400601
display id: annotation2400601
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1

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persistent identity: range2400601
display id: range2400601
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 3129
orientation: inline

C --> T
type: SequenceAnnotation
persistent identity: annotation2400598
display id: annotation2400598
version: 1
top level: BBa_K1468004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1

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persistent identity: range2400598
display id: range2400598
version: 1
direction: direction/forward
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start: 3085
end: 3085
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2400592
display id: annotation2400592
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1

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type: Range
persistent identity: range2400592
display id: range2400592
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 20
end: 25
orientation: inline

LacZ protein
type: SequenceAnnotation
persistent identity: annotation2418063
display id: annotation2418063
version: 1
top level: BBa_K1468004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1

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type: Range
persistent identity: range2418063
display id: range2418063
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2400591
display id: annotation2400591
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1

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type: Range
persistent identity: range2400591
display id: range2400591
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 19
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2400593
display id: annotation2400593
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1

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type: Range
persistent identity: range2400593
display id: range2400593
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 54
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2400599
display id: annotation2400599
version: 1
top level: BBa_K1468004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1

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type: Range
persistent identity: range2400599
display id: range2400599
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3129
end: 3129
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2400600
display id: annotation2400600
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1
type: Range
persistent identity: range2400600
display id: range2400600
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2400597
display id: annotation2400597
version: 1
top level: BBa_K1468004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1

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type: Range
persistent identity: range2400597
display id: range2400597
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 63
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2400594
display id: annotation2400594
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1
type: Range
persistent identity: range2400594
display id: range2400594
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 26
end: 44
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2400596
display id: annotation2400596
version: 1
top level: BBa_K1468004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1
type: Range
persistent identity: range2400596
display id: range2400596
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2400595
display id: annotation2400595
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1
type: Range
persistent identity: range2400595
display id: range2400595
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 43
end: 48
orientation: inline

BBa_K801012
type: ComponentDefinition
persistent identity: BBa K801012
display id: BBa_K801012
version: 1
was generated by: iGEM to SBOL conversion
description: ADH1 yeast terminator
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: ADH1 terminator of ''S. cerevisiae''
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Georg Sch??tzinger
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ADH1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801012_sequence/1

ADH1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202145
display id: annotation2202145
version: 1
top level: BBa_K801012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1

https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1
type: Range
persistent identity: range2202145
display id: range2202145
version: 1
top level: BBa_K801012
direction: direction/forward
start: 1
end: 349
orientation: inline

BBa_K1486009
type: ComponentDefinition
persistent identity: BBa K1486009
display id: BBa_K1486009
version: 1
was generated by: iGEM to SBOL conversion
description: CxpR & Split IFP1.4 [Nterm + Nterm]
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486007
component: BBa_K1486006
component: BBa_I0500
component: BBa_K1486052
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486007
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486052
sequence: https://synbiohub.org/public/igem/BBa_K1486009_sequence/1

BBa_K1486004
type: Component
persistent identity: component2420758
display id: component2420758
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486004

BBa_K1486000
type: Component
persistent identity: component2420766
display id: component2420766
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2420767
display id: component2420767
version: 1
top level: BBa_K1486009
access: public
definition: BBa_B0034

BBa_B0034
type: Component
persistent identity: component2420779
display id: component2420779
version: 1
top level: BBa_K1486009
access: public
definition: BBa_B0034

BBa_K1486007
type: Component
persistent identity: component2420783
display id: component2420783
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486007

BBa_K1486006
type: Component
persistent identity: component2420771
display id: component2420771
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486006

BBa_K1486000
type: Component
persistent identity: component2420788
display id: component2420788
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_K1486000
type: Component
persistent identity: component2420776
display id: component2420776
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_K1486004
type: Component
persistent identity: component2420772
display id: component2420772
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486004

BBa_K1486004
type: Component
persistent identity: component2420784
display id: component2420784
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486004

BBa_K1486007
type: Component
persistent identity: component2420756
display id: component2420756
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486007

BBa_I0500
type: Component
persistent identity: component2420763
display id: component2420763
version: 1
top level: BBa_K1486009
access: public
definition: pBad/araC

BBa_K1486052
type: Component
persistent identity: component2420777
display id: component2420777
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486052

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2420771
display id: annotation2420771
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420771/range2420771/1
component: BBa_K1486006

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type: Range
persistent identity: range2420771
display id: range2420771
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 1223
end: 1627
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420756
display id: annotation2420756
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420756/range2420756/1
component: BBa_K1486007

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type: Range
persistent identity: range2420756
display id: range2420756
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 2410
end: 2994
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2420763
display id: annotation2420763
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420763/range2420763/1
component: BBa_I0500

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type: Range
persistent identity: range2420763
display id: range2420763
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 1
end: 1210
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420783
display id: annotation2420783
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420783/range2420783/1
component: BBa_K1486007

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type: Range
persistent identity: range2420783
display id: range2420783
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 2410
end: 2994
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420767
display id: annotation2420767
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420767/range2420767/1
component: BBa_B0034

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type: Range
persistent identity: range2420767
display id: range2420767
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420758
display id: annotation2420758
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420758/range2420758/1
component: BBa_K1486004

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type: Range
persistent identity: range2420758
display id: range2420758
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 2995
end: 3024
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420779
display id: annotation2420779
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420779/range2420779/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420779/range2420779/1
type: Range
persistent identity: range2420779
display id: range2420779
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 2398
end: 2409
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420772
display id: annotation2420772
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420772/range2420772/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420772/range2420772/1
type: Range
persistent identity: range2420772
display id: range2420772
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 1628
end: 1657
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420766
display id: annotation2420766
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420766/range2420766/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420766/range2420766/1
type: Range
persistent identity: range2420766
display id: range2420766
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 3025
end: 3726
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420788
display id: annotation2420788
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420788/range2420788/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420788/range2420788/1
type: Range
persistent identity: range2420788
display id: range2420788
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 3025
end: 3726
orientation: inline

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2420777
display id: annotation2420777
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420777/range2420777/1
component: BBa_K1486052

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420777/range2420777/1
type: Range
persistent identity: range2420777
display id: range2420777
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 2360
end: 2397
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420776
display id: annotation2420776
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420776/range2420776/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420776/range2420776/1
type: Range
persistent identity: range2420776
display id: range2420776
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 1658
end: 2359
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420784
display id: annotation2420784
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420784/range2420784/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420784/range2420784/1
type: Range
persistent identity: range2420784
display id: range2420784
version: 1
top level: BBa_K1486009
direction: direction/reverse
start: 2995
end: 3024
orientation: inline

BBa_B0016
type: ComponentDefinition
persistent identity: BBa B0016
display id: BBa_B0016
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (T7 RNAP specific, T_Phi)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 gene 1 specific transcriptional terminator
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0016_sequence/1

BBa_B0023
type: ComponentDefinition
persistent identity: BBa B0023
display id: BBa_B0023
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphage T7, reversed
created: 2003-10-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is the reverse of terminator B0013, a bacteriophage T7 terminator which was engineered to be bidirectional. Contains a poly A insertion upstream of a 20 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0023_sequence/1

BBa_K864600
type: ComponentDefinition
persistent identity: BBa K864600
display id: BBa_K864600
version: 1
was generated by: iGEM to SBOL conversion
description: T0 (21 imm) transcriptional terminator
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
mutable provenance: Gene synthesis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T0 transcriptional terminator terminates transcription with 80-90% efficiency.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Erik Lundin
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: T0 terminator
sequence: https://synbiohub.org/public/igem/BBa_K864600_sequence/1

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2197273
display id: annotation2197273
version: 1
top level: BBa_K864600
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864600/annotation2197273/range2197273/1

https://synbiohub.org/public/igem/BBa_K864600/annotation2197273/range2197273/1
type: Range
persistent identity: range2197273
display id: range2197273
version: 1
top level: BBa_K864600
direction: direction/forward
start: 1
end: 52
orientation: inline

BBa_K1159307
type: ComponentDefinition
persistent identity: BBa K1159307
display id: BBa_K1159307
version: 1
was generated by: iGEM to SBOL conversion
description: 35S Terminator of Cauliflower Mosaic Virus (CaMV)
creator: TU Munich 2013
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:46:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains the t35S terminator of the Cauliflower Mosaic Virus (CaMV), a plant virus. This part is flanked by RFC[10] pre- and suffix.
mutable notes: x
mutable provenance: Cauliflower Mosaic Virus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: CaMV 35S Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1159307_sequence/1

CaMV 35S Terminator
type: SequenceAnnotation
persistent identity: annotation2341614
display id: annotation2341614
version: 1
top level: BBa_K1159307
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1159307/annotation2341614/range2341614/1

https://synbiohub.org/public/igem/BBa_K1159307/annotation2341614/range2341614/1
type: Range
persistent identity: range2341614
display id: range2341614
version: 1
direction: direction/forward
top level: BBa_K1159307
start: 1
end: 217
orientation: inline

BBa_K1486025
type: ComponentDefinition
persistent identity: BBa K1486025
display id: BBa_K1486025
version: 1
was generated by: iGEM to SBOL conversion
description: ADH1 Terminator
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Adh1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1486025_sequence/1

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation2418776
display id: annotation2418776
version: 1
top level: BBa_K1486025
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486025/annotation2418776/range2418776/1

https://synbiohub.org/public/igem/BBa_K1486025/annotation2418776/range2418776/1
type: Range
persistent identity: range2418776
display id: range2418776
version: 1
direction: direction/forward
top level: BBa_K1486025
start: 1
end: 188
orientation: inline

BBa_K1994045
type: ComponentDefinition
persistent identity: BBa K1994045
display id: BBa_K1994045
version: 1
was generated by: iGEM to SBOL conversion
description: T7 terminator SBa_000587
creator: Egheosa Ogbomo
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T12:31:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2461_
m user id: 30870
owner id: 28134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: sgRNA with PAM-Proximal complementary sequence 6
mutable notes: sgRNA with PAM-Proximal complementary sequence 6
mutable provenance: sgRNA with PAM-Proximal complementary sequence 6
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Spacer
sequence annotation: T7 Terminator
sequence annotation: spacer
sequence: https://synbiohub.org/public/igem/BBa_K1994045_sequence/1

Spacer
type: SequenceAnnotation
persistent identity: annotation2521969
display id: annotation2521969
version: 1
top level: BBa_K1994045
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1994045/annotation2521969/range2521969/1

https://synbiohub.org/public/igem/BBa_K1994045/annotation2521969/range2521969/1
type: Range
persistent identity: range2521969
display id: range2521969
version: 1
direction: direction/forward
top level: BBa_K1994045
start: 53
end: 57
orientation: inline

T7 Terminator
type: SequenceAnnotation
persistent identity: annotation2521968
display id: annotation2521968
version: 1
top level: BBa_K1994045
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1994045/annotation2521968/range2521968/1

https://synbiohub.org/public/igem/BBa_K1994045/annotation2521968/range2521968/1
type: Range
persistent identity: range2521968
display id: range2521968
version: 1
direction: direction/forward
top level: BBa_K1994045
start: 4
end: 52
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation2521935
display id: annotation2521935
version: 1
top level: BBa_K1994045
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1994045/annotation2521935/range2521935/1

https://synbiohub.org/public/igem/BBa_K1994045/annotation2521935/range2521935/1
type: Range
persistent identity: range2521935
display id: range2521935
version: 1
direction: direction/forward
top level: BBa_K1994045
start: 1
end: 3
orientation: inline

BBa_K1031917
type: ComponentDefinition
persistent identity: BBa K1031917
display id: BBa_K1031917
version: 1
was generated by: iGEM to SBOL conversion
description: J23117-XylS-Terminator
created: 2013-10-13T11:00:00Z
modified: 2015-05-08T01:08:48Z
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
mutable description: 117-XylS-TT
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: XylS
sequence annotation: J23117
sequence: https://synbiohub.org/public/igem/BBa_K1031917_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2369602
display id: annotation2369602
version: 1
top level: BBa_K1031917
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369602/range2369602/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369602/range2369602/1
type: Range
persistent identity: range2369602
display id: range2369602
version: 1
top level: BBa_K1031917
direction: direction/forward
start: 1035
end: 1163
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2369601
display id: annotation2369601
version: 1
top level: BBa_K1031917
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369601/range2369601/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369601/range2369601/1
type: Range
persistent identity: range2369601
display id: range2369601
version: 1
top level: BBa_K1031917
direction: direction/forward
start: 63
end: 1028
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2369600
display id: annotation2369600
version: 1
top level: BBa_K1031917
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369600/range2369600/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369600/range2369600/1
type: Range
persistent identity: range2369600
display id: range2369600
version: 1
top level: BBa_K1031917
direction: direction/forward
start: 1
end: 35
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K1031222
type: ComponentDefinition
persistent identity: BBa K1031222
display id: BBa_K1031222
version: 1
was generated by: iGEM to SBOL conversion
description: Po-B0032-sfGFP-Terminator (DmpR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Po-32-sfGFP-TT (DmpR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0032
sequence annotation: B0015
sequence annotation: po promoter
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031222_sequence/1

B0032
type: SequenceAnnotation
persistent identity: annotation2354355
display id: annotation2354355
version: 1
top level: BBa_K1031222
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354355/range2354355/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354355/range2354355/1
type: Range
persistent identity: range2354355
display id: range2354355
version: 1
top level: BBa_K1031222
direction: direction/forward
start: 180
end: 182
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2354357
display id: annotation2354357
version: 1
top level: BBa_K1031222
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354357/range2354357/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354357/range2354357/1
type: Range
persistent identity: range2354357
display id: range2354357
version: 1
top level: BBa_K1031222
direction: direction/forward
start: 930
end: 1058
orientation: inline

po promoter
type: SequenceAnnotation
persistent identity: annotation2354354
display id: annotation2354354
version: 1
top level: BBa_K1031222
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354354/range2354354/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354354/range2354354/1
type: Range
persistent identity: range2354354
display id: range2354354
version: 1
top level: BBa_K1031222
direction: direction/forward
start: 1
end: 171
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354356
display id: annotation2354356
version: 1
top level: BBa_K1031222
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354356/range2354356/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354356/range2354356/1
type: Range
persistent identity: range2354356
display id: range2354356
version: 1
top level: BBa_K1031222
direction: direction/forward
start: 199
end: 921
orientation: inline

BBa_K1031521
type: ComponentDefinition
persistent identity: BBa K1031521
display id: BBa_K1031521
version: 1
was generated by: iGEM to SBOL conversion
description: Pg-B0032-sfGFP-Terminator (HpaR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Pg-32-sfGFP-TT (HpaR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0032
sequence annotation: Pg (HpaR)
sequence annotation: B0015
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031521_sequence/1

B0032
type: SequenceAnnotation
persistent identity: annotation2362522
display id: annotation2362522
version: 1
top level: BBa_K1031521
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362522/range2362522/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362522/range2362522/1
type: Range
persistent identity: range2362522
display id: range2362522
version: 1
top level: BBa_K1031521
direction: direction/forward
start: 234
end: 246
orientation: inline

Pg (HpaR)
type: SequenceAnnotation
persistent identity: annotation2362521
display id: annotation2362521
version: 1
top level: BBa_K1031521
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362521/range2362521/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362521/range2362521/1
type: Range
persistent identity: range2362521
display id: range2362521
version: 1
top level: BBa_K1031521
direction: direction/forward
start: 1
end: 227
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362524
display id: annotation2362524
version: 1
top level: BBa_K1031521
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362524/range2362524/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362524/range2362524/1
type: Range
persistent identity: range2362524
display id: range2362524
version: 1
top level: BBa_K1031521
direction: direction/forward
start: 982
end: 1110
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362523
display id: annotation2362523
version: 1
top level: BBa_K1031521
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362523/range2362523/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362523/range2362523/1
type: Range
persistent identity: range2362523
display id: range2362523
version: 1
top level: BBa_K1031521
direction: direction/forward
start: 253
end: 975
orientation: inline

BBa_B1001
type: ComponentDefinition
persistent identity: BBa B1001
display id: BBa_B1001
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artifical, small, %T~=90)
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: myers
owned by: james
mutable description: Artificial terminator, estimated %T>90
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Poly T tail of 9 residues for high %T
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA tail
sequence annotation: B1001
sequence annotation: stem loop
sequence annotation: Poly A tail
sequence: https://synbiohub.org/public/igem/BBa_B1001_sequence/1

PolyA tail
type: SequenceAnnotation
persistent identity: annotation1898381
display id: annotation1898381
version: 1
top level: BBa_B1001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898381/range1898381/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898381/range1898381/1
type: Range
persistent identity: range1898381
display id: range1898381
version: 1
top level: BBa_B1001
direction: direction/reverse
start: 1
end: 9
orientation: inline

B1001
type: SequenceAnnotation
persistent identity: annotation1898382
display id: annotation1898382
version: 1
top level: BBa_B1001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898382/range1898382/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898382/range1898382/1
type: Range
persistent identity: range1898382
display id: range1898382
version: 1
top level: BBa_B1001
direction: direction/forward
start: 1
end: 34
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898379
display id: annotation1898379
version: 1
top level: BBa_B1001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898379/range1898379/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898379/range1898379/1
type: Range
persistent identity: range1898379
display id: range1898379
version: 1
top level: BBa_B1001
direction: direction/forward
start: 10
end: 25
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898380
display id: annotation1898380
version: 1
top level: BBa_B1001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898380/range1898380/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898380/range1898380/1
type: Range
persistent identity: range1898380
display id: range1898380
version: 1
top level: BBa_B1001
direction: direction/reverse
start: 26
end: 34
orientation: inline

BBa_K187025
type: ComponentDefinition
persistent identity: BBa K187025
display id: BBa_K187025
version: 1
was generated by: iGEM to SBOL conversion
description: terminator in pAB, BioBytes plasmid
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:07Z
mutable provenance: The terminator is derived from part b1006. pAB is entered as part K187000.
owned by: james
owned by: myers
mutable description: This part contains a bidirectional, high efficiency terminator, in the BioBytes universal plasmid pAB.See the BioBytes RFC for details on the BioBytes assembly method.
discontinued: false
dominant: true
experience: experience/None
group u list: _286_
m user id: 0
owner id: 4048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: A terminator with long sticky ends can be prepared from this plasmid and assembled downstream of a gene using the Biobytes method. The addition of one DNA segment with the BioBytes method takes only 20 minutes. This ability to easily 'mix and match' terminators and genes allows rapid testing of different terminator designs.
star: false
creator: Julia Pon
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: XbaI
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_K187025_sequence/1

XbaI
type: SequenceAnnotation
persistent identity: annotation2051724
display id: annotation2051724
version: 1
top level: BBa_K187025
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187025/annotation2051724/range2051724/1

https://synbiohub.org/public/igem/BBa_K187025/annotation2051724/range2051724/1
type: Range
persistent identity: range2051724
display id: range2051724
version: 1
top level: BBa_K187025
direction: direction/forward
start: 10
end: 15
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2051725
display id: annotation2051725
version: 1
top level: BBa_K187025
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187025/annotation2051725/range2051725/1

https://synbiohub.org/public/igem/BBa_K187025/annotation2051725/range2051725/1
type: Range
persistent identity: range2051725
display id: range2051725
version: 1
top level: BBa_K187025
direction: direction/forward
start: 53
end: 58
orientation: inline

BBa_I51003
type: ComponentDefinition
persistent identity: BBa I51003
display id: BBa_I51003
version: 1
was generated by: iGEM to SBOL conversion
description: -- No description --
created: 2005-12-01T12:00:00Z
modified: 2015-08-31T04:07:42Z
owned by: james
owned by: myers
mutable description: Super duper part!
discontinued: false
dominant: false
group u list: _47_
m user id: 0
owner id: 52
owning group id: 47
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Drew Endy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: foo binding site
sequence: https://synbiohub.org/public/igem/BBa_I51003_sequence/1

foo binding site
type: SequenceAnnotation
persistent identity: annotation1756359
display id: annotation1756359
version: 1
top level: BBa_I51003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I51003/annotation1756359/range1756359/1

https://synbiohub.org/public/igem/BBa_I51003/annotation1756359/range1756359/1
type: Range
persistent identity: range1756359
display id: range1756359
version: 1
top level: BBa_I51003
direction: direction/forward
start: 10
end: 50
orientation: inline

BBa_K1031803
type: ComponentDefinition
persistent identity: BBa K1031803
display id: BBa_K1031803
version: 1
was generated by: iGEM to SBOL conversion
description: Pu-B0031-sfGFP-Terminator (XylR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Pu-31-sfGFP-TT (XylR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Pu (XylR)
sequence annotation: sfGFP
sequence annotation: B0031
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031803_sequence/1

Pu (XylR)
type: SequenceAnnotation
persistent identity: annotation2362525
display id: annotation2362525
version: 1
top level: BBa_K1031803
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362525/range2362525/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362525/range2362525/1
type: Range
persistent identity: range2362525
display id: range2362525
version: 1
top level: BBa_K1031803
direction: direction/forward
start: 1
end: 180
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362527
display id: annotation2362527
version: 1
top level: BBa_K1031803
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362527/range2362527/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362527/range2362527/1
type: Range
persistent identity: range2362527
display id: range2362527
version: 1
top level: BBa_K1031803
direction: direction/forward
start: 221
end: 929
orientation: inline

B0031
type: SequenceAnnotation
persistent identity: annotation2362526
display id: annotation2362526
version: 1
top level: BBa_K1031803
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362526/range2362526/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362526/range2362526/1
type: Range
persistent identity: range2362526
display id: range2362526
version: 1
top level: BBa_K1031803
direction: direction/forward
start: 187
end: 200
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362528
display id: annotation2362528
version: 1
top level: BBa_K1031803
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362528/range2362528/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362528/range2362528/1
type: Range
persistent identity: range2362528
display id: range2362528
version: 1
top level: BBa_K1031803
direction: direction/forward
start: 936
end: 1064
orientation: inline

BBa_K1392970
type: ComponentDefinition
persistent identity: BBa K1392970
display id: BBa_K1392970
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator+Tetr Promoter+T4 Endolysin
creator: Şeniz Y??ksel
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
group u list: _1769_
m user id: 0
owner id: 16902
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: For this part an artifical Terminator is used (generally added to another parts). The promoter is a TetR repressible promoter.It is sequenced for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog part
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues. High expression of lysozyme only is toxic to bacteria. BBa_R0040 (TetR repressible promoter) is based on a cI promoter. It has been modified to include two TetR binding sites. Constructed by DNA synthesis.
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs BiblioPlus Extension Error fetching PMID 9092630:
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_B1006
component: BBa_K112806
sequence annotation: BBa_B1006
sequence annotation: BBa_K112806
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1392970_sequence/1

BBa_R0040
type: Component
persistent identity: component2415802
display id: component2415802
version: 1
top level: BBa_K1392970
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B1006
type: Component
persistent identity: component2415801
display id: component2415801
version: 1
top level: BBa_K1392970
access: public
definition: BBa_B1006

BBa_K112806
type: Component
persistent identity: component2415808
display id: component2415808
version: 1
top level: BBa_K1392970
access: public
definition: https://synbiohub.org/public/igem/BBa_K112806/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2415801
display id: annotation2415801
version: 1
top level: BBa_K1392970
location: https://synbiohub.org/public/igem/BBa_K1392970/annotation2415801/range2415801/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1392970/annotation2415801/range2415801/1
type: Range
persistent identity: range2415801
display id: range2415801
version: 1
direction: direction/reverse
top level: BBa_K1392970
start: 1
end: 39
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2415808
display id: annotation2415808
version: 1
top level: BBa_K1392970
location: https://synbiohub.org/public/igem/BBa_K1392970/annotation2415808/range2415808/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K1392970/annotation2415808/range2415808/1
type: Range
persistent identity: range2415808
display id: range2415808
version: 1
direction: direction/reverse
top level: BBa_K1392970
start: 110
end: 623
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2415802
display id: annotation2415802
version: 1
top level: BBa_K1392970
location: https://synbiohub.org/public/igem/BBa_K1392970/annotation2415802/range2415802/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1392970/annotation2415802/range2415802/1
type: Range
persistent identity: range2415802
display id: range2415802
version: 1
direction: direction/forward
top level: BBa_K1392970
start: 48
end: 101
orientation: inline

BBa_B1010
type: ComponentDefinition
persistent identity: BBa B1010
display id: BBa_B1010
version: 1
was generated by: iGEM to SBOL conversion
description: Terninator (artificial, large, %T~<10)
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~<10% 8bp stem, 6nt loop Bidirectional, estimated reverse %T~=40%
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 3 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: B1010
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1010_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1901015
display id: annotation1901015
version: 1
top level: BBa_B1010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901015/range1901015/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901015/range1901015/1
type: Range
persistent identity: range1901015
display id: range1901015
version: 1
top level: BBa_B1010
direction: direction/reverse
start: 7
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901016
display id: annotation1901016
version: 1
top level: BBa_B1010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901016/range1901016/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901016/range1901016/1
type: Range
persistent identity: range1901016
display id: range1901016
version: 1
top level: BBa_B1010
direction: direction/forward
start: 32
end: 34
orientation: inline

B1010
type: SequenceAnnotation
persistent identity: annotation1901013
display id: annotation1901013
version: 1
top level: BBa_B1010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901013/range1901013/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901013/range1901013/1
type: Range
persistent identity: range1901013
display id: range1901013
version: 1
top level: BBa_B1010
direction: direction/forward
start: 1
end: 40
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1901014
display id: annotation1901014
version: 1
top level: BBa_B1010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901014/range1901014/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901014/range1901014/1
type: Range
persistent identity: range1901014
display id: range1901014
version: 1
top level: BBa_B1010
direction: direction/forward
start: 10
end: 31
orientation: inline

BBa_C0450
type: ComponentDefinition
persistent identity: BBa C0450
display id: BBa_C0450
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (C0440 + C0440)
created: 2005-10-28T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
mutable description: Two terminators in Bgl operon that is antiterminated by bglG
discontinued: false
dominant: false
group u list: _44_
m user id: 0
owner id: 439
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T. Senthil
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_C0440
sequence annotation: BBa_C0440
sequence: https://synbiohub.org/public/igem/BBa_C0450_sequence/1

BBa_C0440
type: Component
persistent identity: component1744572
display id: component1744572
version: 1
top level: BBa_C0450
access: public
definition: BBa_C0440

BBa_C0440
type: Component
persistent identity: component1744577
display id: component1744577
version: 1
top level: BBa_C0450
access: public
definition: BBa_C0440

BBa_C0440
type: SequenceAnnotation
persistent identity: annotation1744572
display id: annotation1744572
version: 1
top level: BBa_C0450
location: https://synbiohub.org/public/igem/BBa_C0450/annotation1744572/range1744572/1
component: BBa_C0440

https://synbiohub.org/public/igem/BBa_C0450/annotation1744572/range1744572/1
type: Range
persistent identity: range1744572
display id: range1744572
version: 1
top level: BBa_C0450
direction: direction/forward
start: 1
end: 124
orientation: inline

BBa_C0440
type: SequenceAnnotation
persistent identity: annotation1744577
display id: annotation1744577
version: 1
top level: BBa_C0450
location: https://synbiohub.org/public/igem/BBa_C0450/annotation1744577/range1744577/1
component: BBa_C0440

https://synbiohub.org/public/igem/BBa_C0450/annotation1744577/range1744577/1
type: Range
persistent identity: range1744577
display id: range1744577
version: 1
top level: BBa_C0450
direction: direction/forward
start: 133
end: 256
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

BBa_K1899003
type: ComponentDefinition
persistent identity: BBa K1899003
display id: BBa_K1899003
version: 1
was generated by: iGEM to SBOL conversion
description: Superfolder GFP
creator: iGEM2016_HKUST
created: 2016-08-27T11:00:00Z
modified: 2016-10-15T04:03:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Variant of GFP that folds robustly even when fused to poorly folded proteins. Waldo and other research groups has reported the engineering of a superfolder GFP (sfGFP) that showed increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding (Pedelacq et al., 2006; Andrews et al., 2007; Fisher and DeLisa, 2008). sfGFP has proven to be very useful as a scaffold for improved protein detection and tagging both in vivo and in vitro using self-assembled sfGFP fragments (Cabantous et al., 2005b; Cabantous and Waldo, 2006). Furthermore, sfGFP fusions are more soluble than conventional GFP fusions (Wu et al., 2009).
mutable notes: nil
mutable provenance: Waldo lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_B0032
sequence annotation: scar
sequence annotation: BBa_J61048
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1899003_sequence/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2481802
display id: annotation2481802
version: 1
top level: BBa_K1899003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481802/range2481802/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481802/range2481802/1
type: Range
persistent identity: range2481802
display id: range2481802
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 1
end: 12
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2481806
display id: annotation2481806
version: 1
top level: BBa_K1899003
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481806/range2481806/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481806/range2481806/1
type: Range
persistent identity: range2481806
display id: range2481806
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 734
end: 741
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2481804
display id: annotation2481804
version: 1
top level: BBa_K1899003
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481804/range2481804/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481804/range2481804/1
type: Range
persistent identity: range2481804
display id: range2481804
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 13
end: 19
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2481805
display id: annotation2481805
version: 1
top level: BBa_K1899003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481805/range2481805/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481805/range2481805/1
type: Range
persistent identity: range2481805
display id: range2481805
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 742
end: 854
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2481803
display id: annotation2481803
version: 1
top level: BBa_K1899003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481803/range2481803/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481803/range2481803/1
type: Range
persistent identity: range2481803
display id: range2481803
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 20
end: 733
orientation: inline

hGH pA
type: ComponentDefinition
persistent identity: BBa K678018
display id: BBa_K678018
version: 1
was generated by: iGEM to SBOL conversion
description: hGH poly A, terminator for mammalian cells
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: From plasmid N-terminal pFLAG-CMV from Sigma-Aldrich
owned by: james
owned by: myers
mutable description: hGH poly A, terminator for mammalian cells
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: hGH
sequence: https://synbiohub.org/public/igem/BBa_K678018_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127350
display id: annotation2127350
version: 1
top level: hGH pA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678018/annotation2127350/range2127350/1

https://synbiohub.org/public/igem/BBa_K678018/annotation2127350/range2127350/1
type: Range
persistent identity: range2127350
display id: range2127350
version: 1
top level: hGH pA
direction: direction/forward
start: 1
end: 8
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2127351
display id: annotation2127351
version: 1
top level: hGH pA
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678018/annotation2127351/range2127351/1

https://synbiohub.org/public/igem/BBa_K678018/annotation2127351/range2127351/1
type: Range
persistent identity: range2127351
display id: range2127351
version: 1
top level: hGH pA
direction: direction/forward
start: 9
end: 635
orientation: inline

BBa_B0020
type: ComponentDefinition
persistent identity: BBa B0020
display id: BBa_B0020
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0010)
created: 2003-10-07T11:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Antiquity. thought to be T1 transcriptional terminator from E. coli RRNB operon.
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop (B0010), cloned onto the antisense strand.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0020_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation354342
display id: annotation354342
version: 1
top level: BBa_B0020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0020/annotation354342/range354342/1

https://synbiohub.org/public/igem/BBa_B0020/annotation354342/range354342/1
type: Range
persistent identity: range354342
display id: range354342
version: 1
top level: BBa_B0020
direction: direction/forward
start: 27
end: 70
orientation: inline

BBa_K1899001
type: ComponentDefinition
persistent identity: BBa K1899001
display id: BBa_K1899001
version: 1
was generated by: iGEM to SBOL conversion
description: Blue Fluorescent Protein mTagBFP generator (Medium RBS)
creator: iGEM2016_HKUST
created: 2016-09-19T11:00:00Z
modified: 2016-09-21T09:47:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Improved version of BBa_K592023 by adding a terminator BBa_B1006 3???to the part.
mutable notes: NIL
mutable provenance: NIL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592023
component: BBa_B1006
sequence annotation: BBa_B1006
sequence annotation: BBa_K592023
sequence: https://synbiohub.org/public/igem/BBa_K1899001_sequence/1

BBa_K592023
type: Component
persistent identity: component2484331
display id: component2484331
version: 1
top level: BBa_K1899001
access: public
definition: https://synbiohub.org/public/igem/BBa_K592023/1

BBa_B1006
type: Component
persistent identity: component2484336
display id: component2484336
version: 1
top level: BBa_K1899001
access: public
definition: BBa_B1006

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2484336
display id: annotation2484336
version: 1
top level: BBa_K1899001
location: https://synbiohub.org/public/igem/BBa_K1899001/annotation2484336/range2484336/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1899001/annotation2484336/range2484336/1
type: Range
persistent identity: range2484336
display id: range2484336
version: 1
direction: direction/reverse
top level: BBa_K1899001
start: 733
end: 771
orientation: inline

BBa_K592023
type: SequenceAnnotation
persistent identity: annotation2484331
display id: annotation2484331
version: 1
top level: BBa_K1899001
location: https://synbiohub.org/public/igem/BBa_K1899001/annotation2484331/range2484331/1
component: BBa_K592023

https://synbiohub.org/public/igem/BBa_K1899001/annotation2484331/range2484331/1
type: Range
persistent identity: range2484331
display id: range2484331
version: 1
direction: direction/reverse
top level: BBa_K1899001
start: 1
end: 724
orientation: inline

BBa_K1031111
type: ComponentDefinition
persistent identity: BBa K1031111
display id: BBa_K1031111
version: 1
was generated by: iGEM to SBOL conversion
description: J23109-CapR-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis Pseudomonas putida
owned by: james
owned by: myers
mutable description: 109-CapR-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CapR
sequence annotation: B0015
sequence annotation: J23109
sequence: https://synbiohub.org/public/igem/BBa_K1031111_sequence/1

CapR
type: SequenceAnnotation
persistent identity: annotation2361310
display id: annotation2361310
version: 1
top level: BBa_K1031111
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361310/range2361310/1

https://synbiohub.org/public/igem/BBa_K1031111/annotation2361310/range2361310/1
type: Range
persistent identity: range2361310
display id: range2361310
version: 1
top level: BBa_K1031111
direction: direction/forward
start: 62
end: 1753
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361311
display id: annotation2361311
version: 1
top level: BBa_K1031111
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361311/range2361311/1

https://synbiohub.org/public/igem/BBa_K1031111/annotation2361311/range2361311/1
type: Range
persistent identity: range2361311
display id: range2361311
version: 1
top level: BBa_K1031111
direction: direction/forward
start: 1760
end: 1888
orientation: inline

J23109
type: SequenceAnnotation
persistent identity: annotation2361309
display id: annotation2361309
version: 1
top level: BBa_K1031111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361309/range2361309/1

https://synbiohub.org/public/igem/BBa_K1031111/annotation2361309/range2361309/1
type: Range
persistent identity: range2361309
display id: range2361309
version: 1
top level: BBa_K1031111
direction: direction/forward
start: 1
end: 35
orientation: inline

BBa_K1031113
type: ComponentDefinition
persistent identity: BBa K1031113
display id: BBa_K1031113
version: 1
was generated by: iGEM to SBOL conversion
description: Po-B0031-sfGFP-Terminator (CapR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis
owned by: james
owned by: myers
mutable description: Po-31-sfGFP-TT (CapR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Po (CapR)
sequence annotation: B0031
sequence annotation: B0015
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031113_sequence/1

Po (CapR)
type: SequenceAnnotation
persistent identity: annotation2362064
display id: annotation2362064
version: 1
top level: BBa_K1031113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362064/range2362064/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362064/range2362064/1
type: Range
persistent identity: range2362064
display id: range2362064
version: 1
top level: BBa_K1031113
direction: direction/forward
start: 1
end: 170
orientation: inline

B0031
type: SequenceAnnotation
persistent identity: annotation2362065
display id: annotation2362065
version: 1
top level: BBa_K1031113
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362065/range2362065/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362065/range2362065/1
type: Range
persistent identity: range2362065
display id: range2362065
version: 1
top level: BBa_K1031113
direction: direction/forward
start: 177
end: 189
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362067
display id: annotation2362067
version: 1
top level: BBa_K1031113
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362067/range2362067/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362067/range2362067/1
type: Range
persistent identity: range2362067
display id: range2362067
version: 1
top level: BBa_K1031113
direction: direction/forward
start: 925
end: 1054
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362066
display id: annotation2362066
version: 1
top level: BBa_K1031113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362066/range2362066/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362066/range2362066/1
type: Range
persistent identity: range2362066
display id: range2362066
version: 1
top level: BBa_K1031113
direction: direction/forward
start: 197
end: 919
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_K1031931
type: ComponentDefinition
persistent identity: BBa K1031931
display id: BBa_K1031931
version: 1
was generated by: iGEM to SBOL conversion
description: J23114-XylC-Terminator
created: 2013-10-13T11:00:00Z
modified: 2015-05-08T01:08:48Z
mutable provenance: Pseudomonas.putida pWW0
owned by: james
owned by: myers
mutable description: 114-XylC-TT
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: PCR & molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23114
sequence annotation: XylC
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031931_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2369603
display id: annotation2369603
version: 1
top level: BBa_K1031931
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369603/range2369603/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369603/range2369603/1
type: Range
persistent identity: range2369603
display id: range2369603
version: 1
top level: BBa_K1031931
direction: direction/forward
start: 1
end: 35
orientation: inline

XylC
type: SequenceAnnotation
persistent identity: annotation2369604
display id: annotation2369604
version: 1
top level: BBa_K1031931
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369604/range2369604/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369604/range2369604/1
type: Range
persistent identity: range2369604
display id: range2369604
version: 1
top level: BBa_K1031931
direction: direction/forward
start: 54
end: 1517
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2369605
display id: annotation2369605
version: 1
top level: BBa_K1031931
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369605/range2369605/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369605/range2369605/1
type: Range
persistent identity: range2369605
display id: range2369605
version: 1
top level: BBa_K1031931
direction: direction/forward
start: 1518
end: 1646
orientation: inline

BBa_K2122000
type: ComponentDefinition
persistent identity: BBa K2122000
display id: BBa_K2122000
version: 1
was generated by: iGEM to SBOL conversion
description: Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter
created: 2016-09-28T11:00:00Z
modified: 2016-10-18T01:26:30Z
mutable provenance: Coding Sequence from GenBank
owned by: james
owned by: myers
mutable description: This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2591_
m user id: 32074
owner id: 32074
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: An inducible promoter (BBa_10500), RBS (BBa_B0034) Scar was added to the sequence. Along with a terminator.
star: false
creator: Sannia Farrukh
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K2122150
component: BBa_B0034
component: BBa_B0014
component: BBa_I0500
sequence annotation: BBa_B0014
sequence annotation: BBa_B0034
sequence annotation: BBa_K2122150
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K2122000_sequence/1

BBa_K2122150
type: Component
persistent identity: component2517225
display id: component2517225
version: 1
top level: BBa_K2122000
access: public
definition: BBa_K2122150

BBa_B0034
type: Component
persistent identity: component2517224
display id: component2517224
version: 1
top level: BBa_K2122000
access: public
definition: BBa_B0034

BBa_B0014
type: Component
persistent identity: component2517232
display id: component2517232
version: 1
top level: BBa_K2122000
access: public
definition: BBa_B0014

BBa_I0500
type: Component
persistent identity: component2517222
display id: component2517222
version: 1
top level: BBa_K2122000
access: public
definition: pBad/araC

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2517232
display id: annotation2517232
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517232/range2517232/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517232/range2517232/1
type: Range
persistent identity: range2517232
display id: range2517232
version: 1
top level: BBa_K2122000
direction: direction/reverse
start: 2307
end: 2401
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2517224
display id: annotation2517224
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517224/range2517224/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517224/range2517224/1
type: Range
persistent identity: range2517224
display id: range2517224
version: 1
top level: BBa_K2122000
direction: direction/reverse
start: 1219
end: 1230
orientation: inline

BBa_K2122150
type: SequenceAnnotation
persistent identity: annotation2517225
display id: annotation2517225
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517225/range2517225/1
component: BBa_K2122150

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517225/range2517225/1
type: Range
persistent identity: range2517225
display id: range2517225
version: 1
top level: BBa_K2122000
direction: direction/reverse
start: 1237
end: 2298
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2517222
display id: annotation2517222
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517222/range2517222/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517222/range2517222/1
type: Range
persistent identity: range2517222
display id: range2517222
version: 1
top level: BBa_K2122000
direction: direction/reverse
start: 1
end: 1210
orientation: inline

T7stop
type: ComponentDefinition
persistent identity: BBa J18913
display id: BBa_J18913
version: 1
was generated by: iGEM to SBOL conversion
description: T7 terminator (incl. STOP)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression plasmid
owned by: james
owned by: myers
mutable description: T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J18913_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2065587
display id: annotation2065587
version: 1
top level: T7stop
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1

https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
type: Range
persistent identity: range2065587
display id: range2065587
version: 1
top level: T7stop
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1486052
type: ComponentDefinition
persistent identity: BBa K1486052
display id: BBa_K1486052
version: 1
was generated by: iGEM to SBOL conversion
description: Spacer
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: to edit
owned by: james
owned by: myers
mutable description: to edit
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: to edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1486052_sequence/1

BBa_K382024
type: ComponentDefinition
persistent identity: BBa K382024
display id: BBa_K382024
version: 1
was generated by: iGEM to SBOL conversion
description: NosT plant specific terminator w/ Stop codon
creator: Patrick Boyle
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:12:40Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 7814
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Amplified via PCR from pORE vector E3, TGA stop codon added on 5' end
mutable notes: TGA stop codon added
mutable provenance: Derived from the pORE series of vectors (see http://2010.igem.org/Team:Harvard)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K382024_sequence/1

BBa_K1031510
type: ComponentDefinition
persistent identity: BBa K1031510
display id: BBa_K1031510
version: 1
was generated by: iGEM to SBOL conversion
description: HpaR-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
mutable description: HpaR-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HpaR
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031510_sequence/1

HpaR
type: SequenceAnnotation
persistent identity: annotation2357472
display id: annotation2357472
version: 1
top level: BBa_K1031510
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357472/range2357472/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357472/range2357472/1
type: Range
persistent identity: range2357472
display id: range2357472
version: 1
top level: BBa_K1031510
direction: direction/forward
start: 19
end: 465
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357473
display id: annotation2357473
version: 1
top level: BBa_K1031510
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357473/range2357473/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357473/range2357473/1
type: Range
persistent identity: range2357473
display id: range2357473
version: 1
top level: BBa_K1031510
direction: direction/forward
start: 474
end: 602
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357469
display id: annotation2357469
version: 1
top level: BBa_K1031510
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357469/range2357469/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357469/range2357469/1
type: Range
persistent identity: range2357469
display id: range2357469
version: 1
top level: BBa_K1031510
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_K1031916
type: ComponentDefinition
persistent identity: BBa K1031916
display id: BBa_K1031916
version: 1
was generated by: iGEM to SBOL conversion
description: J23114-XylS-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
mutable provenance: synthesis PSEUDOMONAS
owned by: james
owned by: myers
mutable description: 114-XylS-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23114
sequence annotation: XylS
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031916_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2360184
display id: annotation2360184
version: 1
top level: BBa_K1031916
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360184/range2360184/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360184/range2360184/1
type: Range
persistent identity: range2360184
display id: range2360184
version: 1
top level: BBa_K1031916
direction: direction/forward
start: 1
end: 35
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2360185
display id: annotation2360185
version: 1
top level: BBa_K1031916
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360185/range2360185/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360185/range2360185/1
type: Range
persistent identity: range2360185
display id: range2360185
version: 1
top level: BBa_K1031916
direction: direction/forward
start: 62
end: 1027
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360187
display id: annotation2360187
version: 1
top level: BBa_K1031916
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360187/range2360187/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360187/range2360187/1
type: Range
persistent identity: range2360187
display id: range2360187
version: 1
top level: BBa_K1031916
direction: direction/forward
start: 1034
end: 1162
orientation: inline

BBa_K1031301
type: ComponentDefinition
persistent identity: BBa K1031301
display id: BBa_K1031301
version: 1
was generated by: iGEM to SBOL conversion
description: Pc-B0031-sfGFP-Terminator (HbpR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Ph-31-sfGFP-TT (HbpR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_B0015
sequence annotation: sfGFP
sequence annotation: Ph
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_K1031301_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2360429
display id: annotation2360429
version: 1
top level: BBa_K1031301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360429/range2360429/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360429/range2360429/1
type: Range
persistent identity: range2360429
display id: range2360429
version: 1
top level: BBa_K1031301
direction: direction/forward
start: 984
end: 1112
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2360428
display id: annotation2360428
version: 1
top level: BBa_K1031301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360428/range2360428/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360428/range2360428/1
type: Range
persistent identity: range2360428
display id: range2360428
version: 1
top level: BBa_K1031301
direction: direction/forward
start: 255
end: 977
orientation: inline

Ph
type: SequenceAnnotation
persistent identity: annotation2360426
display id: annotation2360426
version: 1
top level: BBa_K1031301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360426/range2360426/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360426/range2360426/1
type: Range
persistent identity: range2360426
display id: range2360426
version: 1
top level: BBa_K1031301
direction: direction/forward
start: 1
end: 229
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2360427
display id: annotation2360427
version: 1
top level: BBa_K1031301
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360427/range2360427/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360427/range2360427/1
type: Range
persistent identity: range2360427
display id: range2360427
version: 1
top level: BBa_K1031301
direction: direction/forward
start: 236
end: 248
orientation: inline

BBa_K1031804
type: ComponentDefinition
persistent identity: BBa K1031804
display id: BBa_K1031804
version: 1
was generated by: iGEM to SBOL conversion
description: Pu-B0032-sfGFP-Terminator (XylR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Pu-32-sfGFP-TT (XylR)
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: B0032
sequence annotation: Pu (XylR)
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031804_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362533
display id: annotation2362533
version: 1
top level: BBa_K1031804
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362533/range2362533/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362533/range2362533/1
type: Range
persistent identity: range2362533
display id: range2362533
version: 1
top level: BBa_K1031804
direction: direction/forward
start: 206
end: 928
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2362532
display id: annotation2362532
version: 1
top level: BBa_K1031804
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362532/range2362532/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362532/range2362532/1
type: Range
persistent identity: range2362532
display id: range2362532
version: 1
top level: BBa_K1031804
direction: direction/forward
start: 187
end: 199
orientation: inline

Pu (XylR)
type: SequenceAnnotation
persistent identity: annotation2362531
display id: annotation2362531
version: 1
top level: BBa_K1031804
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362531/range2362531/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362531/range2362531/1
type: Range
persistent identity: range2362531
display id: range2362531
version: 1
top level: BBa_K1031804
direction: direction/forward
start: 1
end: 180
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362534
display id: annotation2362534
version: 1
top level: BBa_K1031804
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362534/range2362534/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362534/range2362534/1
type: Range
persistent identity: range2362534
display id: range2362534
version: 1
top level: BBa_K1031804
direction: direction/forward
start: 935
end: 1063
orientation: inline

BBa_K1031620
type: ComponentDefinition
persistent identity: BBa K1031620
display id: BBa_K1031620
version: 1
was generated by: iGEM to SBOL conversion
description: NahF-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
mutable description: NahF-TT
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031620_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2360134
display id: annotation2360134
version: 1
top level: BBa_K1031620
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031620/annotation2360134/range2360134/1

https://synbiohub.org/public/igem/BBa_K1031620/annotation2360134/range2360134/1
type: Range
persistent identity: range2360134
display id: range2360134
version: 1
top level: BBa_K1031620
direction: direction/forward
start: 1
end: 1452
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360136
display id: annotation2360136
version: 1
top level: BBa_K1031620
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031620/annotation2360136/range2360136/1

https://synbiohub.org/public/igem/BBa_K1031620/annotation2360136/range2360136/1
type: Range
persistent identity: range2360136
display id: range2360136
version: 1
top level: BBa_K1031620
direction: direction/forward
start: 1459
end: 1587
orientation: inline

BBa_Y1015
type: ComponentDefinition
persistent identity: BBa Y1015
display id: BBa_Y1015
version: 1
was generated by: iGEM to SBOL conversion
description: CycE1
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Terminator
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_Y1015_sequence/1

BBa_K1468002
type: ComponentDefinition
persistent identity: BBa K1468002
display id: BBa_K1468002
version: 1
was generated by: iGEM to SBOL conversion
description: pJ23110 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468002_sequence/1

BBa_K1031923
type: ComponentDefinition
persistent identity: BBa K1031923
display id: BBa_K1031923
version: 1
was generated by: iGEM to SBOL conversion
description: J23109-XylB-Terminator
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:48Z
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
mutable description: J23109-XylB-Terminator
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: J23109
sequence annotation: XylB
sequence: https://synbiohub.org/public/igem/BBa_K1031923_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2370041
display id: annotation2370041
version: 1
top level: BBa_K1031923
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370041/range2370041/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370041/range2370041/1
type: Range
persistent identity: range2370041
display id: range2370041
version: 1
top level: BBa_K1031923
direction: direction/forward
start: 1176
end: 1304
orientation: inline

J23109
type: SequenceAnnotation
persistent identity: annotation2370039
display id: annotation2370039
version: 1
top level: BBa_K1031923
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370039/range2370039/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370039/range2370039/1
type: Range
persistent identity: range2370039
display id: range2370039
version: 1
top level: BBa_K1031923
direction: direction/forward
start: 1
end: 35
orientation: inline

XylB
type: SequenceAnnotation
persistent identity: annotation2370040
display id: annotation2370040
version: 1
top level: BBa_K1031923
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370040/range2370040/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370040/range2370040/1
type: Range
persistent identity: range2370040
display id: range2370040
version: 1
top level: BBa_K1031923
direction: direction/forward
start: 63
end: 1169
orientation: inline

COX3 termi
type: ComponentDefinition
persistent identity: BBa K809005
display id: BBa_K809005
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator of Yeast mitochondrial gene COX3
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region. http://www.yeastgenome.org/
owned by: james
owned by: myers
mutable description: This is the terminator region for COX3 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We modelled the RNA secondary structure of COX3 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K809005_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2194193
display id: annotation2194193
version: 1
top level: COX3 termi
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K809005/annotation2194193/range2194193/1

https://synbiohub.org/public/igem/BBa_K809005/annotation2194193/range2194193/1
type: Range
persistent identity: range2194193
display id: range2194193
version: 1
top level: COX3 termi
direction: direction/forward
start: 16
end: 34
orientation: inline

BBa_B1009
type: ComponentDefinition
persistent identity: BBa B1009
display id: BBa_B1009
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~=40%)
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% * 8bp stem, 6nt loop * Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 4 residues
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1009
sequence annotation: PolyA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1009_sequence/1

B1009
type: SequenceAnnotation
persistent identity: annotation1901009
display id: annotation1901009
version: 1
top level: BBa_B1009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901009/range1901009/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901009/range1901009/1
type: Range
persistent identity: range1901009
display id: range1901009
version: 1
top level: BBa_B1009
direction: direction/forward
start: 1
end: 40
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901011
display id: annotation1901011
version: 1
top level: BBa_B1009
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901011/range1901011/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901011/range1901011/1
type: Range
persistent identity: range1901011
display id: range1901011
version: 1
top level: BBa_B1009
direction: direction/reverse
start: 6
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1901010
display id: annotation1901010
version: 1
top level: BBa_B1009
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901010/range1901010/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901010/range1901010/1
type: Range
persistent identity: range1901010
display id: range1901010
version: 1
top level: BBa_B1009
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901012
display id: annotation1901012
version: 1
top level: BBa_B1009
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901012/range1901012/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901012/range1901012/1
type: Range
persistent identity: range1901012
display id: range1901012
version: 1
top level: BBa_B1009
direction: direction/forward
start: 32
end: 35
orientation: inline

BBa_I11013
type: ComponentDefinition
persistent identity: BBa I11013
display id: BBa_I11013
version: 1
was generated by: iGEM to SBOL conversion
description: Modification of biobricks part BBa_B0015
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Modified BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I11013_sequence/1

Modified BBa_B0015
type: SequenceAnnotation
persistent identity: annotation1064099
display id: annotation1064099
version: 1
top level: BBa_I11013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I11013/annotation1064099/range1064099/1

https://synbiohub.org/public/igem/BBa_I11013/annotation1064099/range1064099/1
type: Range
persistent identity: range1064099
display id: range1064099
version: 1
top level: BBa_I11013
direction: direction/forward
start: 1
end: 129
orientation: inline

BBa_C0440
type: ComponentDefinition
persistent identity: BBa C0440
display id: BBa_C0440
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (bgl)
created: 2005-10-28T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
mutable description: Terminator in Bgl operon that is antiterminated by bglG
discontinued: false
dominant: false
group u list: _44_
m user id: 0
owner id: 439
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T. Senthil
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: bgl terminator
sequence: https://synbiohub.org/public/igem/BBa_C0440_sequence/1

bgl terminator
type: SequenceAnnotation
persistent identity: annotation1744294
display id: annotation1744294
version: 1
top level: BBa_C0440
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_C0440/annotation1744294/range1744294/1

https://synbiohub.org/public/igem/BBa_C0440/annotation1744294/range1744294/1
type: Range
persistent identity: range1744294
display id: range1744294
version: 1
top level: BBa_C0440
direction: direction/forward
start: 68
end: 119
orientation: inline

BBa_B1005
type: ComponentDefinition
persistent identity: BBa B1005
display id: BBa_B1005
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, small, %T~=25%
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~=25% * 6bp stem, 4nt loop * Bidirectional, estimated reverse %T~<10%
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 3 residues. Designed to be a terminator with low efficiency.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem loop
sequence annotation: PolyA
sequence annotation: B1005
sequence: https://synbiohub.org/public/igem/BBa_B1005_sequence/1

stem loop
type: SequenceAnnotation
persistent identity: annotation1898426
display id: annotation1898426
version: 1
top level: BBa_B1005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898426/range1898426/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898426/range1898426/1
type: Range
persistent identity: range1898426
display id: range1898426
version: 1
top level: BBa_B1005
direction: direction/forward
start: 10
end: 25
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898427
display id: annotation1898427
version: 1
top level: BBa_B1005
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898427/range1898427/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898427/range1898427/1
type: Range
persistent identity: range1898427
display id: range1898427
version: 1
top level: BBa_B1005
direction: direction/reverse
start: 7
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898425
display id: annotation1898425
version: 1
top level: BBa_B1005
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898425/range1898425/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898425/range1898425/1
type: Range
persistent identity: range1898425
display id: range1898425
version: 1
top level: BBa_B1005
direction: direction/forward
start: 26
end: 28
orientation: inline

B1005
type: SequenceAnnotation
persistent identity: annotation1898424
display id: annotation1898424
version: 1
top level: BBa_B1005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898424/range1898424/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898424/range1898424/1
type: Range
persistent identity: range1898424
display id: range1898424
version: 1
top level: BBa_B1005
direction: direction/forward
start: 1
end: 34
orientation: inline

BBa_K1031410
type: ComponentDefinition
persistent identity: BBa K1031410
display id: BBa_K1031410
version: 1
was generated by: iGEM to SBOL conversion
description: HcaR-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
mutable description: HcaR-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0015
sequence annotation: HcaR
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031410_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357452
display id: annotation2357452
version: 1
top level: BBa_K1031410
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357452/range2357452/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357452/range2357452/1
type: Range
persistent identity: range2357452
display id: range2357452
version: 1
top level: BBa_K1031410
direction: direction/forward
start: 918
end: 1046
orientation: inline

HcaR
type: SequenceAnnotation
persistent identity: annotation2357451
display id: annotation2357451
version: 1
top level: BBa_K1031410
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357451/range2357451/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357451/range2357451/1
type: Range
persistent identity: range2357451
display id: range2357451
version: 1
top level: BBa_K1031410
direction: direction/forward
start: 19
end: 909
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357450
display id: annotation2357450
version: 1
top level: BBa_K1031410
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357450/range2357450/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357450/range2357450/1
type: Range
persistent identity: range2357450
display id: range2357450
version: 1
top level: BBa_K1031410
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_K1031710
type: ComponentDefinition
persistent identity: BBa K1031710
display id: BBa_K1031710
version: 1
was generated by: iGEM to SBOL conversion
description: PaaX-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
mutable description: PaaX-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0015
sequence annotation: PaaX
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031710_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357487
display id: annotation2357487
version: 1
top level: BBa_K1031710
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357487/range2357487/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357487/range2357487/1
type: Range
persistent identity: range2357487
display id: range2357487
version: 1
top level: BBa_K1031710
direction: direction/forward
start: 978
end: 1106
orientation: inline

PaaX
type: SequenceAnnotation
persistent identity: annotation2357486
display id: annotation2357486
version: 1
top level: BBa_K1031710
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357486/range2357486/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357486/range2357486/1
type: Range
persistent identity: range2357486
display id: range2357486
version: 1
top level: BBa_K1031710
direction: direction/forward
start: 19
end: 969
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357485
display id: annotation2357485
version: 1
top level: BBa_K1031710
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357485/range2357485/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357485/range2357485/1
type: Range
persistent identity: range2357485
display id: range2357485
version: 1
top level: BBa_K1031710
direction: direction/forward
start: 1
end: 12
orientation: inline

trkD
type: ComponentDefinition
persistent identity: BBa K629005
display id: BBa_K629005
version: 1
was generated by: iGEM to SBOL conversion
description: trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
created: 2011-10-03T11:00:00Z
modified: 2015-05-08T01:12:56Z
mutable provenance: ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTAATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGCTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTCCCTATTCGTCTGGCGTGGTTCACTGTCGTATTACCTTCCTTAACTCTTAATTACTTCGGCCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTTTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATCGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Zilong WANG, Yi ZHENG
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rev primer
sequence annotation: terminator
sequence annotation: fwd primer
sequence annotation: start
sequence annotation: TrkD
sequence: https://synbiohub.org/public/igem/BBa_K629005_sequence/1

rev primer
type: SequenceAnnotation
persistent identity: annotation2157757
display id: annotation2157757
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1
type: Range
persistent identity: range2157757
display id: range2157757
version: 1
top level: trkD
direction: direction/reverse
start: 1849
end: 1869
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2157758
display id: annotation2157758
version: 1
top level: trkD
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1
type: Range
persistent identity: range2157758
display id: range2157758
version: 1
top level: trkD
direction: direction/forward
start: 1867
end: 1869
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2157755
display id: annotation2157755
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1
type: Range
persistent identity: range2157755
display id: range2157755
version: 1
top level: trkD
direction: direction/forward
start: 1
end: 21
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2157754
display id: annotation2157754
version: 1
top level: trkD
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1
type: Range
persistent identity: range2157754
display id: range2157754
version: 1
top level: trkD
direction: direction/forward
start: 1
end: 3
orientation: inline

TrkD
type: SequenceAnnotation
persistent identity: annotation2157756
display id: annotation2157756
version: 1
top level: trkD
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1
type: Range
persistent identity: range2157756
display id: range2157756
version: 1
top level: trkD
direction: direction/forward
start: 1
end: 1869
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
access: public
definition: BBa_B0011

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
access: public
definition: BBa_B0012

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_K1031922
type: ComponentDefinition
persistent identity: BBa K1031922
display id: BBa_K1031922
version: 1
was generated by: iGEM to SBOL conversion
description: J23114-XylB-Terminator
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:48Z
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
mutable description: J23114-XylB-Terminator
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylB
sequence annotation: B0015
sequence annotation: J23114
sequence: https://synbiohub.org/public/igem/BBa_K1031922_sequence/1

XylB
type: SequenceAnnotation
persistent identity: annotation2370037
display id: annotation2370037
version: 1
top level: BBa_K1031922
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370037/range2370037/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370037/range2370037/1
type: Range
persistent identity: range2370037
display id: range2370037
version: 1
top level: BBa_K1031922
direction: direction/forward
start: 63
end: 1169
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2370038
display id: annotation2370038
version: 1
top level: BBa_K1031922
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370038/range2370038/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370038/range2370038/1
type: Range
persistent identity: range2370038
display id: range2370038
version: 1
top level: BBa_K1031922
direction: direction/forward
start: 1176
end: 1304
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2370036
display id: annotation2370036
version: 1
top level: BBa_K1031922
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370036/range2370036/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370036/range2370036/1
type: Range
persistent identity: range2370036
display id: range2370036
version: 1
top level: BBa_K1031922
direction: direction/forward
start: 1
end: 35
orientation: inline

Q0255 term
type: ComponentDefinition
persistent identity: BBa K809006
display id: BBa_K809006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator of Yeast mitochondrial gene Q0255
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: We modelled the RNA secondary structure of Q0255 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
owned by: myers
owned by: james
mutable description: This is the terminator region for Q0255 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region. http://www.yeastgenome.org/
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K809006_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2194556
display id: annotation2194556
version: 1
top level: Q0255 term
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K809006/annotation2194556/range2194556/1

https://synbiohub.org/public/igem/BBa_K809006/annotation2194556/range2194556/1
type: Range
persistent identity: range2194556
display id: range2194556
version: 1
top level: Q0255 term
direction: direction/forward
start: 16
end: 37
orientation: inline

BBa_K1468001
type: ComponentDefinition
persistent identity: BBa K1468001
display id: BBa_K1468001
version: 1
was generated by: iGEM to SBOL conversion
description: pJ23104 + gene encoding AsRed2
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468001_sequence/1

BGH pA
type: ComponentDefinition
persistent identity: BBa K678019
display id: BBa_K678019
version: 1
was generated by: iGEM to SBOL conversion
description: BGH poly A, mammalian terminator
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
mutable description: BGH poly A, terminator for use in mammalian cells
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: BGH
sequence: https://synbiohub.org/public/igem/BBa_K678019_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127352
display id: annotation2127352
version: 1
top level: BGH pA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678019/annotation2127352/range2127352/1

https://synbiohub.org/public/igem/BBa_K678019/annotation2127352/range2127352/1
type: Range
persistent identity: range2127352
display id: range2127352
version: 1
top level: BGH pA
direction: direction/forward
start: 1
end: 8
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2127353
display id: annotation2127353
version: 1
top level: BGH pA
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678019/annotation2127353/range2127353/1

https://synbiohub.org/public/igem/BBa_K678019/annotation2127353/range2127353/1
type: Range
persistent identity: range2127353
display id: range2127353
version: 1
top level: BGH pA
direction: direction/forward
start: 9
end: 233
orientation: inline

BBa_K1031623
type: ComponentDefinition
persistent identity: BBa K1031623
display id: BBa_K1031623
version: 1
was generated by: iGEM to SBOL conversion
description: J23113-NahF-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
mutable description: 113-NahF-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: B0015
sequence annotation: J23113
sequence: https://synbiohub.org/public/igem/BBa_K1031623_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2361891
display id: annotation2361891
version: 1
top level: BBa_K1031623
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361891/range2361891/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361891/range2361891/1
type: Range
persistent identity: range2361891
display id: range2361891
version: 1
top level: BBa_K1031623
direction: direction/forward
start: 62
end: 1513
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361894
display id: annotation2361894
version: 1
top level: BBa_K1031623
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361894/range2361894/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361894/range2361894/1
type: Range
persistent identity: range2361894
display id: range2361894
version: 1
top level: BBa_K1031623
direction: direction/forward
start: 1520
end: 1648
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2361890
display id: annotation2361890
version: 1
top level: BBa_K1031623
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361890/range2361890/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361890/range2361890/1
type: Range
persistent identity: range2361890
display id: range2361890
version: 1
top level: BBa_K1031623
direction: direction/forward
start: 1
end: 35
orientation: inline

Reporter +
type: ComponentDefinition
persistent identity: BBa K653001
display id: BBa_K653001
version: 1
was generated by: iGEM to SBOL conversion
description: GFP reporter plus Double Terminator
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:13:00Z
mutable provenance: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtg aaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatgg tgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttc aaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaat tagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattac ctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg atgaactatacaaataataa ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc accttcgggtgggcctttctgcgtttata
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The iGEM Team Panama 2011 have to design and assemble the ends of an expression platform in order to bring up by rational designing a fuctional test platform for the expression of our Team favorite BioBrick part BBa_K653000 A.K. The Biosurfactator. We choose to assembly two existing BioBrick parts from the shop, we mean The Registry. Those parts are: a GFP reporter BBa_E0040 and a double terminator BBa_B0015. We assemble them to bring up a new composite part that will serve as a well characterized ends for a expression platform for several coding sequences like our RhlAB_BBa_K653000
discontinued: false
dominant: false
experience: experience/None
group u list: _830_
m user id: 0
owner id: 7119
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This GFP part was derived from the reporter device used by Elowitz's repressilator : GFPmut3* has a couple of mutations from wtGFP - S2R and S65G and S72A. Emerald and EGFP have S65T and multiple other mutations. The S2R mutation should have no effect. The Double terminator consisting of BBa_B0010 and BBa_B0012. This is the most commonly used terminator. It seems to be reliable. The assembling of this two parts was performed using the 3A Assembly Method.
star: false
creator: Ernesto G??mez
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_E0040
sequence annotation: BBa_B0015
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K653001_sequence/1

BBa_B0015
type: Component
persistent identity: component2146086
display id: component2146086
version: 1
top level: Reporter +
access: public
definition: BBa_B0015

BBa_E0040
type: Component
persistent identity: component2146079
display id: component2146079
version: 1
top level: Reporter +
access: public
definition: GFP

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2146086
display id: annotation2146086
version: 1
top level: Reporter +
location: https://synbiohub.org/public/igem/BBa_K653001/annotation2146086/range2146086/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K653001/annotation2146086/range2146086/1
type: Range
persistent identity: range2146086
display id: range2146086
version: 1
top level: Reporter +
direction: direction/reverse
start: 729
end: 857
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2146079
display id: annotation2146079
version: 1
top level: Reporter +
location: https://synbiohub.org/public/igem/BBa_K653001/annotation2146079/range2146079/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K653001/annotation2146079/range2146079/1
type: Range
persistent identity: range2146079
display id: range2146079
version: 1
top level: Reporter +
direction: direction/reverse
start: 1
end: 720
orientation: inline

BBa_K1031625
type: ComponentDefinition
persistent identity: BBa K1031625
display id: BBa_K1031625
version: 1
was generated by: iGEM to SBOL conversion
description: J23117-NahF-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
mutable description: 117-NahF-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: J23117
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031625_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2361915
display id: annotation2361915
version: 1
top level: BBa_K1031625
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361915/range2361915/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361915/range2361915/1
type: Range
persistent identity: range2361915
display id: range2361915
version: 1
top level: BBa_K1031625
direction: direction/forward
start: 62
end: 1513
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2361913
display id: annotation2361913
version: 1
top level: BBa_K1031625
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361913/range2361913/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361913/range2361913/1
type: Range
persistent identity: range2361913
display id: range2361913
version: 1
top level: BBa_K1031625
direction: direction/forward
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361916
display id: annotation2361916
version: 1
top level: BBa_K1031625
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361916/range2361916/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361916/range2361916/1
type: Range
persistent identity: range2361916
display id: range2361916
version: 1
top level: BBa_K1031625
direction: direction/forward
start: 1520
end: 1648
orientation: inline

BBa_B0060
type: ComponentDefinition
persistent identity: BBa B0060
display id: BBa_B0060
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0050)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Reverse Bi-directional terminator from B0050 (derived from pBR322).
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0060_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318622
display id: annotation318622
version: 1
top level: BBa_B0060
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0060/annotation318622/range318622/1

https://synbiohub.org/public/igem/BBa_B0060/annotation318622/range318622/1
type: Range
persistent identity: range318622
display id: range318622
version: 1
top level: BBa_B0060
direction: direction/forward
start: 10
end: 25
orientation: inline

BBa_K1031223
type: ComponentDefinition
persistent identity: BBa K1031223
display id: BBa_K1031223
version: 1
was generated by: iGEM to SBOL conversion
description: Po-B0033-sfGFP-Terminator (DmpR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Po-33-sfGFP-TT (DmpR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: b0033
sequence annotation: po promoter
sequence annotation: sfGFP
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031223_sequence/1

b0033
type: SequenceAnnotation
persistent identity: annotation2354359
display id: annotation2354359
version: 1
top level: BBa_K1031223
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354359/range2354359/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354359/range2354359/1
type: Range
persistent identity: range2354359
display id: range2354359
version: 1
top level: BBa_K1031223
direction: direction/forward
start: 180
end: 190
orientation: inline

po promoter
type: SequenceAnnotation
persistent identity: annotation2354358
display id: annotation2354358
version: 1
top level: BBa_K1031223
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354358/range2354358/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354358/range2354358/1
type: Range
persistent identity: range2354358
display id: range2354358
version: 1
top level: BBa_K1031223
direction: direction/forward
start: 1
end: 171
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354360
display id: annotation2354360
version: 1
top level: BBa_K1031223
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354360/range2354360/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354360/range2354360/1
type: Range
persistent identity: range2354360
display id: range2354360
version: 1
top level: BBa_K1031223
direction: direction/forward
start: 197
end: 919
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2354361
display id: annotation2354361
version: 1
top level: BBa_K1031223
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354361/range2354361/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354361/range2354361/1
type: Range
persistent identity: range2354361
display id: range2354361
version: 1
top level: BBa_K1031223
direction: direction/forward
start: 928
end: 1056
orientation: inline

BBa_B1004
type: ComponentDefinition
persistent identity: BBa B1004
display id: BBa_B1004
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, small, %T~=55)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
mutable description: Artificial terminator, estimated %T~=55 * 6bp stem, 4nt loop * Bidirectional, estimated reverse %T~<20
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 4 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: stem loop
sequence annotation: B1004
sequence: https://synbiohub.org/public/igem/BBa_B1004_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1898423
display id: annotation1898423
version: 1
top level: BBa_B1004
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898423/range1898423/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898423/range1898423/1
type: Range
persistent identity: range1898423
display id: range1898423
version: 1
top level: BBa_B1004
direction: direction/forward
start: 26
end: 29
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898421
display id: annotation1898421
version: 1
top level: BBa_B1004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898421/range1898421/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898421/range1898421/1
type: Range
persistent identity: range1898421
display id: range1898421
version: 1
top level: BBa_B1004
direction: direction/forward
start: 10
end: 25
orientation: inline

B1004
type: SequenceAnnotation
persistent identity: annotation1898420
display id: annotation1898420
version: 1
top level: BBa_B1004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898420/range1898420/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898420/range1898420/1
type: Range
persistent identity: range1898420
display id: range1898420
version: 1
top level: BBa_B1004
direction: direction/forward
start: 1
end: 34
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898422
display id: annotation1898422
version: 1
top level: BBa_B1004
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898422/range1898422/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898422/range1898422/1
type: Range
persistent identity: range1898422
display id: range1898422
version: 1
top level: BBa_B1004
direction: direction/reverse
start: 6
end: 9
orientation: inline

BBa_K1031114
type: ComponentDefinition
persistent identity: BBa K1031114
display id: BBa_K1031114
version: 1
was generated by: iGEM to SBOL conversion
description: Po-B0032-sfGFP (CapR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Po-32-sfGFP (CapR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: B0032
sequence annotation: Po (CapR)
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031114_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362070
display id: annotation2362070
version: 1
top level: BBa_K1031114
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362070/range2362070/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362070/range2362070/1
type: Range
persistent identity: range2362070
display id: range2362070
version: 1
top level: BBa_K1031114
direction: direction/forward
start: 196
end: 918
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2362069
display id: annotation2362069
version: 1
top level: BBa_K1031114
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362069/range2362069/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362069/range2362069/1
type: Range
persistent identity: range2362069
display id: range2362069
version: 1
top level: BBa_K1031114
direction: direction/forward
start: 177
end: 189
orientation: inline

Po (CapR)
type: SequenceAnnotation
persistent identity: annotation2362068
display id: annotation2362068
version: 1
top level: BBa_K1031114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362068/range2362068/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362068/range2362068/1
type: Range
persistent identity: range2362068
display id: range2362068
version: 1
top level: BBa_K1031114
direction: direction/forward
start: 1
end: 170
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362071
display id: annotation2362071
version: 1
top level: BBa_K1031114
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362071/range2362071/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362071/range2362071/1
type: Range
persistent identity: range2362071
display id: range2362071
version: 1
top level: BBa_K1031114
direction: direction/forward
start: 925
end: 1053
orientation: inline

BBa_K1486006
type: ComponentDefinition
persistent identity: BBa K1486006
display id: BBa_K1486006
version: 1
was generated by: iGEM to SBOL conversion
description: IFP[1]
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
mutable description: This is the first part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[2].
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21033
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When fused to another coding sequence, insert a flexible linker
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: IFP[1]
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1486006_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2418009
display id: annotation2418009
version: 1
top level: BBa_K1486006
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418009/range2418009/1

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418009/range2418009/1
type: Range
persistent identity: range2418009
display id: range2418009
version: 1
top level: BBa_K1486006
direction: direction/forward
start: 1
end: 3
orientation: inline

IFP[1]
type: SequenceAnnotation
persistent identity: annotation2418010
display id: annotation2418010
version: 1
top level: BBa_K1486006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418010/range2418010/1

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418010/range2418010/1
type: Range
persistent identity: range2418010
display id: range2418010
version: 1
top level: BBa_K1486006
direction: direction/forward
start: 1
end: 405
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2418011
display id: annotation2418011
version: 1
top level: BBa_K1486006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418011/range2418011/1

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418011/range2418011/1
type: Range
persistent identity: range2418011
display id: range2418011
version: 1
top level: BBa_K1486006
direction: direction/forward
start: 403
end: 405
orientation: inline

BBa_B0021
type: ComponentDefinition
persistent identity: BBa B0021
display id: BBa_B0021
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-), reversed
created: 2003-10-13T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator cloned in the reverse direction of B0011. 22 bp stem-loop
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Cloned with primer-dimers into pSB1A2
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0021
sequence: https://synbiohub.org/public/igem/BBa_B0021_sequence/1

BBa_B0021
type: SequenceAnnotation
persistent identity: annotation7024
display id: annotation7024
version: 1
top level: BBa_B0021
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0021/annotation7024/range7024/1

https://synbiohub.org/public/igem/BBa_B0021/annotation7024/range7024/1
type: Range
persistent identity: range7024
display id: range7024
version: 1
top level: BBa_B0021
direction: direction/forward
start: 1
end: 46
orientation: inline

BBa_K1840002
type: ComponentDefinition
persistent identity: BBa K1840002
display id: BBa_K1840002
version: 1
was generated by: iGEM to SBOL conversion
description: Zwf promotor from Pseudomonas putida
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T01:47:02Z
mutable provenance: The part was designed after information from the paper by DADDAOUA, A., KRELL, T. & RAMOS, J.-L. 2009. Regulation of Glucose Metabolism in Pseudomonas: The Phosphorylative Branch and Entner-Doudoroff Enzymes are Regulated by a Repressor Containing a Sugar Iisomerase Domain. Journal of Biological Chemistry, 284, 21360-21368.
owned by: james
owned by: myers
mutable description: This basic part is the promotor in front of the gene for zwf (glucose 6-phosphate dehydrogenase) in Pseudomonas putida. Zwf is concerned with the glucose metabolism in P. putida. The zwf promotor is negatively regulated by the repressor called HexR. It has a binding site for glucose derivative 2-keto-3-deoxy-6-phosphogluconate (KDPG) and binding leads to the release from the promotor. For our project (NTNU Trondheim 2015) we integrated the promotor in front of the mCherry gene. Hence we built a glucose detection device, that expresses mCherry according to the glucose (and therefore KDPG) level in the environment. Note: The promotor edd (BBa_K1840000) is repressed by the same repressor HexR. See biobrick parts BBa_K1840001 and BBa_K1840003 for other promotors on operons regulated by glucose derivatives.
discontinued: false
dominant: false
group u list: _2266_
m user id: 26543
owner id: 26543
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The considerations we made regarded the starting and endpoint of the promotor. We only know that it has to be in front of the zwf gene. We assumed it starts directly after the previous gene. This gene codes for the repressor, HexR, and is situated on the opposite strand.
star: false
creator: Julia Anna Adrian
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1840002_sequence/1

BBa_B0017
type: ComponentDefinition
persistent identity: BBa B0017
display id: BBa_B0017
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0010)
created: 2004-01-08T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator with two copies of <bb_part>BBa_B0010</bb_part>
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Austin Che
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0010
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0017_sequence/1

BBa_B0010
type: Component
persistent identity: component939337
display id: component939337
version: 1
top level: BBa_B0017
access: public
definition: BBa_B0010

BBa_B0010
type: Component
persistent identity: component939331
display id: component939331
version: 1
top level: BBa_B0017
access: public
definition: BBa_B0010

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939337
display id: annotation939337
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
type: Range
persistent identity: range939337
display id: range939337
version: 1
top level: BBa_B0017
direction: direction/forward
start: 89
end: 168
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939331
display id: annotation939331
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
type: Range
persistent identity: range939331
display id: range939331
version: 1
top level: BBa_B0017
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0013
type: ComponentDefinition
persistent identity: BBa B0013
display id: BBa_B0013
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphage T7 (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 V01146 .
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream. Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: polya
sequence annotation: C
sequence annotation: ''
sequence annotation: BBa_B0013
sequence annotation: T7 TE
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0013_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation1698
display id: annotation1698
version: 1
top level: BBa_B0013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1
type: Range
persistent identity: range1698
display id: range1698
version: 1
top level: BBa_B0013
direction: direction/forward
start: 34
end: 47
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1695
display id: annotation1695
version: 1
top level: BBa_B0013
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1
type: Range
persistent identity: range1695
display id: range1695
version: 1
top level: BBa_B0013
direction: direction/forward
start: 37
end: 37
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1696
display id: annotation1696
version: 1
top level: BBa_B0013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1
type: Range
persistent identity: range1696
display id: range1696
version: 1
top level: BBa_B0013
direction: direction/forward
start: 10
end: 10
orientation: inline

''
type: SequenceAnnotation
persistent identity: annotation1697
display id: annotation1697
version: 1
top level: BBa_B0013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1
type: Range
persistent identity: range1697
display id: range1697
version: 1
top level: BBa_B0013
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_B0013
type: SequenceAnnotation
persistent identity: annotation7021
display id: annotation7021
version: 1
top level: BBa_B0013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1

https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1
type: Range
persistent identity: range7021
display id: range7021
version: 1
top level: BBa_B0013
direction: direction/forward
start: 1
end: 47
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1691
display id: annotation1691
version: 1
top level: BBa_B0013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1
type: Range
persistent identity: range1691
display id: range1691
version: 1
top level: BBa_B0013
direction: direction/forward
start: 14
end: 33
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1692
display id: annotation1692
version: 1
top level: BBa_B0013
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1
type: Range
persistent identity: range1692
display id: range1692
version: 1
top level: BBa_B0013
direction: direction/forward
start: 40
end: 40
orientation: inline

T22
type: ComponentDefinition
persistent identity: BBa K864501
display id: BBa_K864501
version: 1
was generated by: iGEM to SBOL conversion
description: T22, P22 late terminator
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
mutable provenance: Salmonella phage P22
owned by: james
owned by: myers
mutable description: Strong transcriptional terminator consisting of a 20 bp stem-loop from Salmonella phage P22.
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 10137
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Design based on McDowell et al 1994.
star: false
creator: Erik Gullberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K864501_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2197326
display id: annotation2197326
version: 1
top level: T22
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864501/annotation2197326/range2197326/1

https://synbiohub.org/public/igem/BBa_K864501/annotation2197326/range2197326/1
type: Range
persistent identity: range2197326
display id: range2197326
version: 1
top level: T22
direction: direction/forward
start: 5
end: 34
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K1031112
type: ComponentDefinition
persistent identity: BBa K1031112
display id: BBa_K1031112
version: 1
was generated by: iGEM to SBOL conversion
description: J23113-CapR-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis Pseudomonas putida
owned by: james
owned by: myers
mutable description: 113-CapR-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: CapR
sequence annotation: J23113
sequence: https://synbiohub.org/public/igem/BBa_K1031112_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2361508
display id: annotation2361508
version: 1
top level: BBa_K1031112
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361508/range2361508/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361508/range2361508/1
type: Range
persistent identity: range2361508
display id: range2361508
version: 1
top level: BBa_K1031112
direction: direction/forward
start: 1760
end: 1888
orientation: inline

CapR
type: SequenceAnnotation
persistent identity: annotation2361507
display id: annotation2361507
version: 1
top level: BBa_K1031112
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361507/range2361507/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361507/range2361507/1
type: Range
persistent identity: range2361507
display id: range2361507
version: 1
top level: BBa_K1031112
direction: direction/forward
start: 62
end: 1753
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2361448
display id: annotation2361448
version: 1
top level: BBa_K1031112
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361448/range2361448/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361448/range2361448/1
type: Range
persistent identity: range2361448
display id: range2361448
version: 1
top level: BBa_K1031112
direction: direction/forward
start: 1
end: 35
orientation: inline

MaTif
type: ComponentDefinition
persistent identity: BBa K2127007
display id: BBa_K2127007
version: 1
was generated by: iGEM to SBOL conversion
description: MT - TERMINATOR
creator: Mirat Sojitra
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T05:23:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2596_
m user id: 32415
owner id: 32736
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MT - TERMINATOR
mutable notes: MT - TERMINATOR
mutable provenance: MT - TERMINATOR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K2127007_sequence/1

BBa_K382023
type: ComponentDefinition
persistent identity: BBa K382023
display id: BBa_K382023
version: 1
was generated by: iGEM to SBOL conversion
description: NosT plant specific terminator
creator: Patrick Boyle
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:13:05Z
discontinued: false
dominant: true
group u list: _488_
m user id: 4206
owner id: 7814
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plant type transcriptional terminator NosT.
mutable notes: Amplified via PCR from pORE vector E3
mutable provenance: Derived from the pORE series of vectors (see http://2010.igem.org/Team:Harvard)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K382023_sequence/1

BBa_K1031520
type: ComponentDefinition
persistent identity: BBa K1031520
display id: BBa_K1031520
version: 1
was generated by: iGEM to SBOL conversion
description: Pg-B0031-sfGFP-Terminator (HpaR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Pg-sfGFP-TT (HpaR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Pg (HpaR)
sequence annotation: B0031
sequence annotation: sfGFP
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031520_sequence/1

Pg (HpaR)
type: SequenceAnnotation
persistent identity: annotation2362409
display id: annotation2362409
version: 1
top level: BBa_K1031520
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362409/range2362409/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362409/range2362409/1
type: Range
persistent identity: range2362409
display id: range2362409
version: 1
top level: BBa_K1031520
direction: direction/forward
start: 1
end: 227
orientation: inline

B0031
type: SequenceAnnotation
persistent identity: annotation2362410
display id: annotation2362410
version: 1
top level: BBa_K1031520
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362410/range2362410/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362410/range2362410/1
type: Range
persistent identity: range2362410
display id: range2362410
version: 1
top level: BBa_K1031520
direction: direction/forward
start: 234
end: 247
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362411
display id: annotation2362411
version: 1
top level: BBa_K1031520
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362411/range2362411/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362411/range2362411/1
type: Range
persistent identity: range2362411
display id: range2362411
version: 1
top level: BBa_K1031520
direction: direction/forward
start: 254
end: 976
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362412
display id: annotation2362412
version: 1
top level: BBa_K1031520
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362412/range2362412/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362412/range2362412/1
type: Range
persistent identity: range2362412
display id: range2362412
version: 1
top level: BBa_K1031520
direction: direction/forward
start: 983
end: 1111
orientation: inline

BBa_K1994043
type: ComponentDefinition
persistent identity: BBa K1994043
display id: BBa_K1994043
version: 1
was generated by: iGEM to SBOL conversion
description: Truncated B0010 terminator
creator: Egheosa Ogbomo
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:01:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2461_
m user id: 30870
owner id: 28134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: sgRNA with PAM-Proximal complementary sequence 4
mutable notes: sgRNA with PAM-Proximal complementary sequence 4
mutable provenance: sgRNA with PAM-Proximal complementary sequence 4
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0010 terminator
sequence annotation: Stop codons
sequence: https://synbiohub.org/public/igem/BBa_K1994043_sequence/1

B0010 terminator
type: SequenceAnnotation
persistent identity: annotation2522946
display id: annotation2522946
version: 1
top level: BBa_K1994043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1994043/annotation2522946/range2522946/1

https://synbiohub.org/public/igem/BBa_K1994043/annotation2522946/range2522946/1
type: Range
persistent identity: range2522946
display id: range2522946
version: 1
direction: direction/forward
top level: BBa_K1994043
start: 15
end: 33
orientation: inline

Stop codons
type: SequenceAnnotation
persistent identity: annotation2522943
display id: annotation2522943
version: 1
top level: BBa_K1994043
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1994043/annotation2522943/range2522943/1

https://synbiohub.org/public/igem/BBa_K1994043/annotation2522943/range2522943/1
type: Range
persistent identity: range2522943
display id: range2522943
version: 1
direction: direction/forward
top level: BBa_K1994043
start: 1
end: 7
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0012

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_K2042007
type: ComponentDefinition
persistent identity: BBa K2042007
display id: BBa_K2042007
version: 1
was generated by: iGEM to SBOL conversion
description: P.putida Terminator
creator: Maria Bargu??s i Ribera
created: 2016-09-01T11:00:00Z
modified: 2016-10-08T02:06:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31380
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K2042007_sequence/1

BBa_B0052
type: ComponentDefinition
persistent identity: BBa B0052
display id: BBa_B0052
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (rrnC)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli ribosonal RNA rrnC operon. This terminator occurs in the reverse orientation upstream of the multiple cloning site of the pSB**1-pSB**3 series of plasmids.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0052_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318601
display id: annotation318601
version: 1
top level: BBa_B0052
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0052/annotation318601/range318601/1

https://synbiohub.org/public/igem/BBa_B0052/annotation318601/range318601/1
type: Range
persistent identity: range318601
display id: range318601
version: 1
top level: BBa_B0052
direction: direction/forward
start: 9
end: 28
orientation: inline

BBa_K731722
type: ComponentDefinition
persistent identity: BBa K731722
display id: BBa_K731722
version: 1
was generated by: iGEM to SBOL conversion
description: T1 terminator from E. coli rrnB
created: 2012-08-22T11:00:00Z
modified: 2015-05-08T01:13:06Z
mutable provenance: This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the B0010 part extracted by PCR from the doulbe terminator B0012. We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use! The characterization has been done both with T7 and Coli RNApolymerases.
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12063
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: I don't have any design considerations, anyway it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.
star: false
creator: Anna Depetris, Giacomo Giacomelli
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0010
sequence: https://synbiohub.org/public/igem/BBa_K731722_sequence/1

B0010
type: SequenceAnnotation
persistent identity: annotation2180631
display id: annotation2180631
version: 1
top level: BBa_K731722
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K731722/annotation2180631/range2180631/1

https://synbiohub.org/public/igem/BBa_K731722/annotation2180631/range2180631/1
type: Range
persistent identity: range2180631
display id: range2180631
version: 1
top level: BBa_K731722
direction: direction/forward
start: 1
end: 79
orientation: inline

BBa_B0062
type: ComponentDefinition
persistent identity: BBa B0062
display id: BBa_B0062
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0052)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0062_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318637
display id: annotation318637
version: 1
top level: BBa_B0062
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1

https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
type: Range
persistent identity: range318637
display id: range318637
version: 1
top level: BBa_B0062
direction: direction/forward
start: 14
end: 33
orientation: inline

BBa_B1008
type: ComponentDefinition
persistent identity: BBa B1008
display id: BBa_B1008
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~=70)
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pair
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~=70% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~=80%
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 5 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: B1008
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1008_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1901007
display id: annotation1901007
version: 1
top level: BBa_B1008
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901007/range1901007/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901007/range1901007/1
type: Range
persistent identity: range1901007
display id: range1901007
version: 1
top level: BBa_B1008
direction: direction/reverse
start: 5
end: 9
orientation: inline

B1008
type: SequenceAnnotation
persistent identity: annotation1901005
display id: annotation1901005
version: 1
top level: BBa_B1008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901005/range1901005/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901005/range1901005/1
type: Range
persistent identity: range1901005
display id: range1901005
version: 1
top level: BBa_B1008
direction: direction/forward
start: 1
end: 40
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901008
display id: annotation1901008
version: 1
top level: BBa_B1008
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901008/range1901008/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901008/range1901008/1
type: Range
persistent identity: range1901008
display id: range1901008
version: 1
top level: BBa_B1008
direction: direction/forward
start: 32
end: 36
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1901006
display id: annotation1901006
version: 1
top level: BBa_B1008
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901006/range1901006/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901006/range1901006/1
type: Range
persistent identity: range1901006
display id: range1901006
version: 1
top level: BBa_B1008
direction: direction/forward
start: 10
end: 31
orientation: inline

BBa_K933000
type: ComponentDefinition
persistent identity: BBa K933000
display id: BBa_K933000
version: 1
was generated by: iGEM to SBOL conversion
description: two start codons coding for RFP and sfGFP reporters
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:47Z
mutable provenance: This part contains no direct BioBrick parts. It was assembled from several pieces of plasmid DNA in the Salis Lab at Penn State. The dRBS1 vector and pBAC reporter components were assembled from PCR-amplified fragments.
owned by: james
owned by: myers
mutable description: This part was used in a design that tests multiple start codons. The first start codon codes for RFP. The second start codon codes for sfGFP. They are 7 nucleotides apart on the DNA strand. The ribosome binding site translation initiation rate determines which protein is expressed. The part has two restriction enzyme sites flanking the RBS and promoter regions, so varying RBS strengths may be tested.
discontinued: false
dominant: false
experience: experience/None
group u list: _1198_
m user id: 0
owner id: 13034
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This design used gBlocks synthesized by IDT. Several CBAR reactions were necessary to achieve the construct.
star: false
creator: Hannah Jepsen-Burger
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: 1N break in reference frame
sequence annotation: 41
sequence annotation: start codon RFP (ORF 0)
sequence annotation: double terminator
sequence annotation: start codon sfGFP (ORF +1)
sequence annotation: sfGFP reporter
sequence annotation: weak RBS
sequence: https://synbiohub.org/public/igem/BBa_K933000_sequence/1

1N break in reference frame
type: SequenceAnnotation
persistent identity: annotation2211998
display id: annotation2211998
version: 1
top level: BBa_K933000
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211998/range2211998/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211998/range2211998/1
type: Range
persistent identity: range2211998
display id: range2211998
version: 1
top level: BBa_K933000
direction: direction/forward
start: 710
end: 710
orientation: inline

41
type: SequenceAnnotation
persistent identity: annotation2211997
display id: annotation2211997
version: 1
top level: BBa_K933000
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211997/range2211997/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211997/range2211997/1
type: Range
persistent identity: range2211997
display id: range2211997
version: 1
top level: BBa_K933000
direction: direction/forward
start: 41
end: 709
orientation: inline

start codon RFP (ORF 0)
type: SequenceAnnotation
persistent identity: annotation2211995
display id: annotation2211995
version: 1
top level: BBa_K933000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211995/range2211995/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211995/range2211995/1
type: Range
persistent identity: range2211995
display id: range2211995
version: 1
top level: BBa_K933000
direction: direction/forward
start: 29
end: 30
orientation: inline

double terminator
type: SequenceAnnotation
persistent identity: annotation2212000
display id: annotation2212000
version: 1
top level: BBa_K933000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2212000/range2212000/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2212000/range2212000/1
type: Range
persistent identity: range2212000
display id: range2212000
version: 1
top level: BBa_K933000
direction: direction/forward
start: 1461
end: 1589
orientation: inline

start codon sfGFP (ORF +1)
type: SequenceAnnotation
persistent identity: annotation2211996
display id: annotation2211996
version: 1
top level: BBa_K933000
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211996/range2211996/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211996/range2211996/1
type: Range
persistent identity: range2211996
display id: range2211996
version: 1
top level: BBa_K933000
direction: direction/forward
start: 39
end: 40
orientation: inline

sfGFP reporter
type: SequenceAnnotation
persistent identity: annotation2211999
display id: annotation2211999
version: 1
top level: BBa_K933000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211999/range2211999/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211999/range2211999/1
type: Range
persistent identity: range2211999
display id: range2211999
version: 1
top level: BBa_K933000
direction: direction/forward
start: 711
end: 1427
orientation: inline

weak RBS
type: SequenceAnnotation
persistent identity: annotation2211994
display id: annotation2211994
version: 1
top level: BBa_K933000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211994/range2211994/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211994/range2211994/1
type: Range
persistent identity: range2211994
display id: range2211994
version: 1
top level: BBa_K933000
direction: direction/forward
start: 1
end: 28
orientation: inline

BBa_B0063
type: ComponentDefinition
persistent identity: BBa B0063
display id: BBa_B0063
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0053)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: Reverse of B0053, E.coli transcriptional terminator from the His operon.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0063_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318639
display id: annotation318639
version: 1
top level: BBa_B0063
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0063/annotation318639/range318639/1

https://synbiohub.org/public/igem/BBa_B0063/annotation318639/range318639/1
type: Range
persistent identity: range318639
display id: range318639
version: 1
top level: BBa_B0063
direction: direction/forward
start: 15
end: 64
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PC
sequence annotation: Pbad
sequence annotation: AraC
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

BBa_B0061
type: ComponentDefinition
persistent identity: BBa B0061
display id: BBa_B0061
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0051)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Reverse of bi-directional terminator B0051. (E.coli derived, genbank U24206 bases 2694-2727. between converging yciA and tonA genes.)
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0061_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318635
display id: annotation318635
version: 1
top level: BBa_B0061
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0061/annotation318635/range318635/1

https://synbiohub.org/public/igem/BBa_B0061/annotation318635/range318635/1
type: Range
persistent identity: range318635
display id: range318635
version: 1
top level: BBa_B0061
direction: direction/forward
start: 10
end: 25
orientation: inline

BBa_B0051
type: ComponentDefinition
persistent identity: BBa B0051
display id: BBa_B0051
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (yciA/tonA, +/-)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: myers
owned by: james
mutable description: Bi-directional Terminator from pSMART HCKAN. E.coli derived, genbank U24206 bases 2694-2727 between yciA and tonA genes (converging transcripts)
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0051_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318600
display id: annotation318600
version: 1
top level: BBa_B0051
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0051/annotation318600/range318600/1

https://synbiohub.org/public/igem/BBa_B0051/annotation318600/range318600/1
type: Range
persistent identity: range318600
display id: range318600
version: 1
top level: BBa_B0051
direction: direction/forward
start: 11
end: 26
orientation: inline

BBa_K1031622
type: ComponentDefinition
persistent identity: BBa K1031622
display id: BBa_K1031622
version: 1
was generated by: iGEM to SBOL conversion
description: J23106-NahF-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
mutable description: 106-NahF-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: J23106
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031622_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2361879
display id: annotation2361879
version: 1
top level: BBa_K1031622
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361879/range2361879/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361879/range2361879/1
type: Range
persistent identity: range2361879
display id: range2361879
version: 1
top level: BBa_K1031622
direction: direction/forward
start: 62
end: 1513
orientation: inline

J23106
type: SequenceAnnotation
persistent identity: annotation2361878
display id: annotation2361878
version: 1
top level: BBa_K1031622
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361878/range2361878/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361878/range2361878/1
type: Range
persistent identity: range2361878
display id: range2361878
version: 1
top level: BBa_K1031622
direction: direction/forward
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361881
display id: annotation2361881
version: 1
top level: BBa_K1031622
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361881/range2361881/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361881/range2361881/1
type: Range
persistent identity: range2361881
display id: range2361881
version: 1
top level: BBa_K1031622
direction: direction/forward
start: 1519
end: 1648
orientation: inline

BBa_K1486005
type: ComponentDefinition
persistent identity: BBa K1486005
display id: BBa_K1486005
version: 1
was generated by: iGEM to SBOL conversion
description: Arabinose promoter + sfGFP-CpxR [Cterm]
created: 2014-08-12T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486000
component: BBa_I0500
component: BBa_K1486003
component: BBa_I746916
component: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_I746916
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486003
sequence: https://synbiohub.org/public/igem/BBa_K1486005_sequence/1

BBa_K1486000
type: Component
persistent identity: component2382088
display id: component2382088
version: 1
top level: BBa_K1486005
access: public
definition: BBa_K1486000

BBa_I0500
type: Component
persistent identity: component2382082
display id: component2382082
version: 1
top level: BBa_K1486005
access: public
definition: pBad/araC

BBa_K1486003
type: Component
persistent identity: component2382089
display id: component2382089
version: 1
top level: BBa_K1486005
access: public
definition: BBa_K1486003

BBa_I746916
type: Component
persistent identity: component2382093
display id: component2382093
version: 1
top level: BBa_K1486005
access: public
definition: BBa_I746916

BBa_B0034
type: Component
persistent identity: component2382084
display id: component2382084
version: 1
top level: BBa_K1486005
access: public
definition: BBa_B0034

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382082
display id: annotation2382082
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
type: Range
persistent identity: range2382082
display id: range2382082
version: 1
top level: BBa_K1486005
direction: direction/reverse
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382088
display id: annotation2382088
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
type: Range
persistent identity: range2382088
display id: range2382088
version: 1
top level: BBa_K1486005
direction: direction/reverse
start: 1223
end: 1924
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382093
display id: annotation2382093
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
type: Range
persistent identity: range2382093
display id: range2382093
version: 1
top level: BBa_K1486005
direction: direction/reverse
start: 1949
end: 2668
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382084
display id: annotation2382084
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
type: Range
persistent identity: range2382084
display id: range2382084
version: 1
top level: BBa_K1486005
direction: direction/reverse
start: 1211
end: 1222
orientation: inline

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382089
display id: annotation2382089
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382089/range2382089/1
component: BBa_K1486003

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382089/range2382089/1
type: Range
persistent identity: range2382089
display id: range2382089
version: 1
top level: BBa_K1486005
direction: direction/reverse
start: 1925
end: 1948
orientation: inline

BBa_B0025
type: ComponentDefinition
persistent identity: BBa B0025
display id: BBa_B0025
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0015), reversed
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0012
sequence annotation: B0010
sequence: https://synbiohub.org/public/igem/BBa_B0025_sequence/1

B0012
type: SequenceAnnotation
persistent identity: annotation369702
display id: annotation369702
version: 1
top level: BBa_B0025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1

https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
type: Range
persistent identity: range369702
display id: range369702
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 1
end: 41
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation369703
display id: annotation369703
version: 1
top level: BBa_B0025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1

https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
type: Range
persistent identity: range369703
display id: range369703
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 50
end: 129
orientation: inline

BBa_K1031621
type: ComponentDefinition
persistent identity: BBa K1031621
display id: BBa_K1031621
version: 1
was generated by: iGEM to SBOL conversion
description: J23105-NahF-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
mutable description: 105-NahF-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: J23105
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031621_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2361695
display id: annotation2361695
version: 1
top level: BBa_K1031621
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361695/range2361695/1

https://synbiohub.org/public/igem/BBa_K1031621/annotation2361695/range2361695/1
type: Range
persistent identity: range2361695
display id: range2361695
version: 1
top level: BBa_K1031621
direction: direction/forward
start: 62
end: 1513
orientation: inline

J23105
type: SequenceAnnotation
persistent identity: annotation2361694
display id: annotation2361694
version: 1
top level: BBa_K1031621
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361694/range2361694/1

https://synbiohub.org/public/igem/BBa_K1031621/annotation2361694/range2361694/1
type: Range
persistent identity: range2361694
display id: range2361694
version: 1
top level: BBa_K1031621
direction: direction/forward
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361696
display id: annotation2361696
version: 1
top level: BBa_K1031621
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361696/range2361696/1

https://synbiohub.org/public/igem/BBa_K1031621/annotation2361696/range2361696/1
type: Range
persistent identity: range2361696
display id: range2361696
version: 1
top level: BBa_K1031621
direction: direction/forward
start: 1520
end: 1648
orientation: inline

BBa_K1486000
type: ComponentDefinition
persistent identity: BBa K1486000
display id: BBa_K1486000
version: 1
was generated by: iGEM to SBOL conversion
description: CpxR coding region
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: The part has been extracted from the E coli genome.
owned by: james
owned by: myers
mutable description: CpxR is a transciption factor that dimerizes and becomes active when phosphorylated by the transmembrane histidine kinase CpxA. CpxA in turn becomes active when the bacterial cell is submitted to different kinds of shock (pH, temperature, pressure, protein misfolding in the inter-membrane space...).
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When making composite parts with CpxR that are supposed to dimerize, we had to take care about the positioning of the other elements regarding the parallel dimerization of CpxR.
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: STOP
sequence annotation: CpxR
sequence: https://synbiohub.org/public/igem/BBa_K1486000_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2382022
display id: annotation2382022
version: 1
top level: BBa_K1486000
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382022/range2382022/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382022/range2382022/1
type: Range
persistent identity: range2382022
display id: range2382022
version: 1
top level: BBa_K1486000
direction: direction/forward
start: 1
end: 3
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2382023
display id: annotation2382023
version: 1
top level: BBa_K1486000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382023/range2382023/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382023/range2382023/1
type: Range
persistent identity: range2382023
display id: range2382023
version: 1
top level: BBa_K1486000
direction: direction/forward
start: 699
end: 702
orientation: inline

CpxR
type: SequenceAnnotation
persistent identity: annotation2382021
display id: annotation2382021
version: 1
top level: BBa_K1486000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382021/range2382021/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382021/range2382021/1
type: Range
persistent identity: range2382021
display id: range2382021
version: 1
top level: BBa_K1486000
direction: direction/forward
start: 1
end: 702
orientation: inline

BBa_K1031913
type: ComponentDefinition
persistent identity: BBa K1031913
display id: BBa_K1031913
version: 1
was generated by: iGEM to SBOL conversion
description: J23109-XylS-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
mutable description: 109-XylS-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23109
sequence annotation: XylS
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031913_sequence/1

J23109
type: SequenceAnnotation
persistent identity: annotation2360169
display id: annotation2360169
version: 1
top level: BBa_K1031913
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360169/range2360169/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360169/range2360169/1
type: Range
persistent identity: range2360169
display id: range2360169
version: 1
top level: BBa_K1031913
direction: direction/forward
start: 1
end: 35
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2360170
display id: annotation2360170
version: 1
top level: BBa_K1031913
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360170/range2360170/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360170/range2360170/1
type: Range
persistent identity: range2360170
display id: range2360170
version: 1
top level: BBa_K1031913
direction: direction/forward
start: 36
end: 1027
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360171
display id: annotation2360171
version: 1
top level: BBa_K1031913
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360171/range2360171/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360171/range2360171/1
type: Range
persistent identity: range2360171
display id: range2360171
version: 1
top level: BBa_K1031913
direction: direction/forward
start: 1034
end: 1162
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 V01146 .
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_K1031420
type: ComponentDefinition
persistent identity: BBa K1031420
display id: BBa_K1031420
version: 1
was generated by: iGEM to SBOL conversion
description: Ph-B0031-sfGFP-Terminator (HcaR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Ph-sfGFP-TT (HcaR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: B0015
sequence annotation: Ph (HcaR)
sequence annotation: B0031
sequence: https://synbiohub.org/public/igem/BBa_K1031420_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362326
display id: annotation2362326
version: 1
top level: BBa_K1031420
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362326/range2362326/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362326/range2362326/1
type: Range
persistent identity: range2362326
display id: range2362326
version: 1
top level: BBa_K1031420
direction: direction/forward
start: 101
end: 823
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362327
display id: annotation2362327
version: 1
top level: BBa_K1031420
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362327/range2362327/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362327/range2362327/1
type: Range
persistent identity: range2362327
display id: range2362327
version: 1
top level: BBa_K1031420
direction: direction/forward
start: 830
end: 958
orientation: inline

Ph (HcaR)
type: SequenceAnnotation
persistent identity: annotation2362324
display id: annotation2362324
version: 1
top level: BBa_K1031420
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362324/range2362324/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362324/range2362324/1
type: Range
persistent identity: range2362324
display id: range2362324
version: 1
top level: BBa_K1031420
direction: direction/forward
start: 1
end: 74
orientation: inline

B0031
type: SequenceAnnotation
persistent identity: annotation2362325
display id: annotation2362325
version: 1
top level: BBa_K1031420
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362325/range2362325/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362325/range2362325/1
type: Range
persistent identity: range2362325
display id: range2362325
version: 1
top level: BBa_K1031420
direction: direction/forward
start: 81
end: 94
orientation: inline

BBa_K110012
type: ComponentDefinition
persistent identity: BBa K110012
display id: BBa_K110012
version: 1
was generated by: iGEM to SBOL conversion
description: STE2 terminator
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
mutable provenance: SGD
owned by: james
owned by: myers
mutable description: This is the exact region between the end of the ORF of STE2-W and the beginning of BST1-C
discontinued: false
dominant: false
experience: experience/Issues
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence was taken from the yeast genome
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Terminator
sequence: https://synbiohub.org/public/igem/BBa_K110012_sequence/1

Terminator
type: SequenceAnnotation
persistent identity: annotation1994877
display id: annotation1994877
version: 1
top level: BBa_K110012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K110012/annotation1994877/range1994877/1

https://synbiohub.org/public/igem/BBa_K110012/annotation1994877/range1994877/1
type: Range
persistent identity: range1994877
display id: range1994877
version: 1
top level: BBa_K110012
direction: direction/forward
start: 1
end: 123
orientation: inline

BBa_K2122150
type: ComponentDefinition
persistent identity: BBa K2122150
display id: BBa_K2122150
version: 1
was generated by: iGEM to SBOL conversion
description: Sequence for Gb3 Synthase
created: 2016-10-17T11:00:00Z
modified: 2016-10-18T10:21:40Z
mutable provenance: GenBank
owned by: james
owned by: myers
mutable description: This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
discontinued: false
dominant: false
group u list: _2591_
m user id: 32074
owner id: 32074
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Sannia Farrukh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2122150_sequence/1

BBa_K1031221
type: ComponentDefinition
persistent identity: BBa K1031221
display id: BBa_K1031221
version: 1
was generated by: iGEM to SBOL conversion
description: Po-B0031-sfGFP-Terminator (DmpR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Po-31-sfGFP-TT (DmpR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0031
sequence annotation: Po promoter
sequence annotation: B0015
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031221_sequence/1

B0031
type: SequenceAnnotation
persistent identity: annotation2354351
display id: annotation2354351
version: 1
top level: BBa_K1031221
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354351/range2354351/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354351/range2354351/1
type: Range
persistent identity: range2354351
display id: range2354351
version: 1
top level: BBa_K1031221
direction: direction/forward
start: 180
end: 193
orientation: inline

Po promoter
type: SequenceAnnotation
persistent identity: annotation2354350
display id: annotation2354350
version: 1
top level: BBa_K1031221
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354350/range2354350/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354350/range2354350/1
type: Range
persistent identity: range2354350
display id: range2354350
version: 1
top level: BBa_K1031221
direction: direction/forward
start: 1
end: 171
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2354353
display id: annotation2354353
version: 1
top level: BBa_K1031221
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354353/range2354353/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354353/range2354353/1
type: Range
persistent identity: range2354353
display id: range2354353
version: 1
top level: BBa_K1031221
direction: direction/forward
start: 931
end: 1059
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354352
display id: annotation2354352
version: 1
top level: BBa_K1031221
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354352/range2354352/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354352/range2354352/1
type: Range
persistent identity: range2354352
display id: range2354352
version: 1
top level: BBa_K1031221
direction: direction/forward
start: 200
end: 922
orientation: inline

BBa_K1899002
type: ComponentDefinition
persistent identity: BBa K1899002
display id: BBa_K1899002
version: 1
was generated by: iGEM to SBOL conversion
description: Blue Fluorescent Protein mTagBFP generator (Strong RBS)
creator: iGEM2016_HKUST
created: 2016-09-20T11:00:00Z
modified: 2016-09-21T09:47:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Improved version of BBa_K592024 by adding a terminator BBa_B1006 3??? to this part.
mutable notes: NIL
mutable provenance: NIL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592024
component: BBa_B1006
sequence annotation: BBa_K592024
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K1899002_sequence/1

BBa_K592024
type: Component
persistent identity: component2484356
display id: component2484356
version: 1
top level: BBa_K1899002
access: public
definition: https://synbiohub.org/public/igem/BBa_K592024/1

BBa_B1006
type: Component
persistent identity: component2484361
display id: component2484361
version: 1
top level: BBa_K1899002
access: public
definition: BBa_B1006

BBa_K592024
type: SequenceAnnotation
persistent identity: annotation2484356
display id: annotation2484356
version: 1
top level: BBa_K1899002
location: https://synbiohub.org/public/igem/BBa_K1899002/annotation2484356/range2484356/1
component: BBa_K592024

https://synbiohub.org/public/igem/BBa_K1899002/annotation2484356/range2484356/1
type: Range
persistent identity: range2484356
display id: range2484356
version: 1
direction: direction/reverse
top level: BBa_K1899002
start: 1
end: 723
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2484361
display id: annotation2484361
version: 1
top level: BBa_K1899002
location: https://synbiohub.org/public/igem/BBa_K1899002/annotation2484361/range2484361/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1899002/annotation2484361/range2484361/1
type: Range
persistent identity: range2484361
display id: range2484361
version: 1
direction: direction/reverse
top level: BBa_K1899002
start: 732
end: 770
orientation: inline

BBa_K1486003
type: ComponentDefinition
persistent identity: BBa K1486003
display id: BBa_K1486003
version: 1
was generated by: iGEM to SBOL conversion
description: Flexible linker 2x (GGGS)
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: Flexible linker used to fuse two proteins.
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Designed considering the flexibility and separation needed between our fused proteins.
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1486003_sequence/1

Zwf-Device
type: ComponentDefinition
persistent identity: BBa K1840007
display id: BBa_K1840007
version: 1
was generated by: iGEM to SBOL conversion
description: Device: TT-zwf-mCherry
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T02:16:57Z
mutable provenance: The double terminator was already registered in the iGEM catalogue. The zwf promotor comes from the genome of Pseudomonas putida. The mCherry is codon optimized for expression in Pseudomonas.
owned by: james
owned by: myers
mutable description: This device acts as a detector for glucose (and certain derivatives of it). The double transcription terminator ensures, that only the gene downstream of the promotor zwf is influenced by it. The zwf promotor (BBa_K1840002) is contained in the genome of Pseudomonas putida and is situated in front of the enzyme zwf (glucose 6-phosphate dehydrogenase). The repressor HexR can bind the zwf promotor sequence and thus prevent the transcription of the zwf gene. In case the glucose derivative 2-Keto-3-deoxy-6-phosphogluconate is present, it binds to the promotor and leads to a subsequent conformational change. Hence, the repressor can no longer bind and the gene(s) downstream can be transcribed. In our device, the downstream gene is mCherry, codon optimized for Pseudomonas (BBa_K1840004). Therefore, in the presence of glucose, mCherry is expressed. In various experiments, we could see that this device is in deed working.
discontinued: false
dominant: false
experience: experience/None
group u list: _2266_
m user id: 26543
owner id: 26543
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: As promotor sequence, we assumed all DNA in front of the zwf gene up to the next gene, coding for the repressor HexR. Since it is encoded on the opposite strand, there is the possibility, that zwf functions as a promotor for this gene as well. Therefore the double terminator was needed.
star: false
creator: Julia Anna Adrian
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1840002
component: BBa_B0014
component: BBa_K1840004
sequence annotation: BBa_K1840002
sequence annotation: BBa_B0014
sequence annotation: BBa_K1840004
sequence: https://synbiohub.org/public/igem/BBa_K1840007_sequence/1

BBa_K1840002
type: Component
persistent identity: component2473635
display id: component2473635
version: 1
top level: Zwf-Device
access: public
definition: BBa_K1840002

BBa_B0014
type: Component
persistent identity: component2473634
display id: component2473634
version: 1
top level: Zwf-Device
access: public
definition: BBa_B0014

BBa_K1840004
type: Component
persistent identity: component2473636
display id: component2473636
version: 1
top level: Zwf-Device
access: public
definition: BBa_K1840004

BBa_K1840002
type: SequenceAnnotation
persistent identity: annotation2473635
display id: annotation2473635
version: 1
top level: Zwf-Device
location: https://synbiohub.org/public/igem/BBa_K1840007/annotation2473635/range2473635/1
component: BBa_K1840002

https://synbiohub.org/public/igem/BBa_K1840007/annotation2473635/range2473635/1
type: Range
persistent identity: range2473635
display id: range2473635
version: 1
top level: Zwf-Device
direction: direction/reverse
start: 104
end: 305
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2473634
display id: annotation2473634
version: 1
top level: Zwf-Device
location: https://synbiohub.org/public/igem/BBa_K1840007/annotation2473634/range2473634/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K1840007/annotation2473634/range2473634/1
type: Range
persistent identity: range2473634
display id: range2473634
version: 1
top level: Zwf-Device
direction: direction/reverse
start: 1
end: 95
orientation: inline

BBa_K1840004
type: SequenceAnnotation
persistent identity: annotation2473636
display id: annotation2473636
version: 1
top level: Zwf-Device
location: https://synbiohub.org/public/igem/BBa_K1840007/annotation2473636/range2473636/1
component: BBa_K1840004

https://synbiohub.org/public/igem/BBa_K1840007/annotation2473636/range2473636/1
type: Range
persistent identity: range2473636
display id: range2473636
version: 1
top level: Zwf-Device
direction: direction/reverse
start: 312
end: 1025
orientation: inline

BBa_K1486001
type: ComponentDefinition
persistent identity: BBa K1486001
display id: BBa_K1486001
version: 1
was generated by: iGEM to SBOL conversion
description: Arabinose promoter + CpxR
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: The CpxR comes from the genome of E coli, and the arabinose promoter comes from a biobrick.
owned by: james
owned by: myers
mutable description: The part contains CpxR with an upstream arabinose-inducible promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The arabinose had to be upstream of the CpxR. Also, the sequence had to be tested because of a mutation, and it works perfectly.
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1486000
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486001_sequence/1

BBa_K1486000
type: Component
persistent identity: component2382078
display id: component2382078
version: 1
top level: BBa_K1486001
access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2382074
display id: component2382074
version: 1
top level: BBa_K1486001
access: public
definition: BBa_B0034

BBa_I0500
type: Component
persistent identity: component2382072
display id: component2382072
version: 1
top level: BBa_K1486001
access: public
definition: pBad/araC

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382072
display id: annotation2382072
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
type: Range
persistent identity: range2382072
display id: range2382072
version: 1
top level: BBa_K1486001
direction: direction/reverse
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382078
display id: annotation2382078
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
type: Range
persistent identity: range2382078
display id: range2382078
version: 1
top level: BBa_K1486001
direction: direction/reverse
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382074
display id: annotation2382074
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
type: Range
persistent identity: range2382074
display id: range2382074
version: 1
top level: BBa_K1486001
direction: direction/reverse
start: 1211
end: 1222
orientation: inline

BBa_K1840004
type: ComponentDefinition
persistent identity: BBa K1840004
display id: BBa_K1840004
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry for Pseudomonas
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:02:21Z
mutable provenance: It comes from the standard mCherry sequence and was condon optimized.
owned by: james
owned by: myers
mutable description: The standard fluorescent protein mCherry is not efficiently expressed in Pseudomonas spp. Therefore this biobrick part is a codon optimized mCherry for the expression in Pseudomonas. We used it in our project (NTNU Trondheim 2015) in a device as reporter. Note: See biobrick parts BBa_K1840005 to BBa_K1840008 for the characterization of the devices.
discontinued: false
dominant: false
group u list: _2266_
m user id: 26543
owner id: 26543
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The expression mechanism in Pseudomonas had to be considered to be able to codon optimize it.
star: false
creator: Julia Anna Adrian
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1840004_sequence/1

BBa_J52016
type: ComponentDefinition
persistent identity: BBa J52016
display id: BBa_J52016
version: 1
was generated by: iGEM to SBOL conversion
description: eukaryotic -- derived from SV40 early poly A signal sequence
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It is cloned in BioBrick vector pSB1AK3.
star: false
creator: Monika Ciglic
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_J52016_sequence/1

terminator
type: SequenceAnnotation
persistent identity: annotation1902869
display id: annotation1902869
version: 1
top level: BBa_J52016
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52016/annotation1902869/range1902869/1

https://synbiohub.org/public/igem/BBa_J52016/annotation1902869/range1902869/1
type: Range
persistent identity: range1902869
display id: range1902869
version: 1
top level: BBa_J52016
direction: direction/forward
start: 1
end: 238
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1902870
display id: annotation1902870
version: 1
top level: BBa_J52016
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_J52016/annotation1902870/range1902870/1

https://synbiohub.org/public/igem/BBa_J52016/annotation1902870/range1902870/1
type: Range
persistent identity: range1902870
display id: range1902870
version: 1
top level: BBa_J52016
direction: direction/forward
start: 1
end: 238
orientation: inline

BBa_B0024
type: ComponentDefinition
persistent identity: BBa B0024
display id: BBa_B0024
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011), reversed
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0024_sequence/1

BBa_K404108
type: ComponentDefinition
persistent identity: BBa K404108
display id: BBa_K404108
version: 1
was generated by: iGEM to SBOL conversion
description: hGH terminator
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
mutable provenance: x
owned by: james
owned by: myers
mutable description: x
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: x
star: false
creator: Freiburg Bioware 2010
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: hGH terminator
sequence: https://synbiohub.org/public/igem/BBa_K404108_sequence/1

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2093173
display id: annotation2093173
version: 1
top level: BBa_K404108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404108/annotation2093173/range2093173/1

https://synbiohub.org/public/igem/BBa_K404108/annotation2093173/range2093173/1
type: Range
persistent identity: range2093173
display id: range2093173
version: 1
top level: BBa_K404108
direction: direction/forward
start: 1
end: 481
orientation: inline

BBa_K404116
type: ComponentDefinition
persistent identity: BBa K404116
display id: BBa_K404116
version: 1
was generated by: iGEM to SBOL conversion
description: hGH_[AAV2]-right-ITR
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
mutable provenance: x
owned by: james
owned by: myers
mutable description: x
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: x
star: false
creator: Freiburg Bioware 2010
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: hGH
sequence annotation: right ITR
sequence: https://synbiohub.org/public/igem/BBa_K404116_sequence/1

hGH
type: SequenceAnnotation
persistent identity: annotation2093227
display id: annotation2093227
version: 1
top level: BBa_K404116
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404116/annotation2093227/range2093227/1

https://synbiohub.org/public/igem/BBa_K404116/annotation2093227/range2093227/1
type: Range
persistent identity: range2093227
display id: range2093227
version: 1
top level: BBa_K404116
direction: direction/forward
start: 1
end: 482
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093229
display id: annotation2093229
version: 1
top level: BBa_K404116
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404116/annotation2093229/range2093229/1

https://synbiohub.org/public/igem/BBa_K404116/annotation2093229/range2093229/1
type: Range
persistent identity: range2093229
display id: range2093229
version: 1
top level: BBa_K404116
direction: direction/forward
start: 495
end: 632
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri AF170104 .
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

BBa_K678037
type: ComponentDefinition
persistent identity: BBa K678037
display id: BBa_K678037
version: 1
was generated by: iGEM to SBOL conversion
description: T1-motni, terminator for Aspergillus niger
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: Aspergillus niger genomic DNA
owned by: james
owned by: myers
mutable description: T1-motni is terminator that works in the fungus Aspergillus niger
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: T1-motni terminator
sequence: https://synbiohub.org/public/igem/BBa_K678037_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127406
display id: annotation2127406
version: 1
top level: BBa_K678037
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678037/annotation2127406/range2127406/1

https://synbiohub.org/public/igem/BBa_K678037/annotation2127406/range2127406/1
type: Range
persistent identity: range2127406
display id: range2127406
version: 1
top level: BBa_K678037
direction: direction/forward
start: 1
end: 8
orientation: inline

T1-motni terminator
type: SequenceAnnotation
persistent identity: annotation2127405
display id: annotation2127405
version: 1
top level: BBa_K678037
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678037/annotation2127405/range2127405/1

https://synbiohub.org/public/igem/BBa_K678037/annotation2127405/range2127405/1
type: Range
persistent identity: range2127405
display id: range2127405
version: 1
top level: BBa_K678037
direction: direction/forward
start: 9
end: 1006
orientation: inline

BBa_K1486007
type: ComponentDefinition
persistent identity: BBa K1486007
display id: BBa_K1486007
version: 1
was generated by: iGEM to SBOL conversion
description: IFP[2]
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
mutable description: This is the second part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[1].
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When fused to another coding sequence, insert a flexible linker.
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: IFP[2]
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1486007_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2382128
display id: annotation2382128
version: 1
top level: BBa_K1486007
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382128/range2382128/1

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382128/range2382128/1
type: Range
persistent identity: range2382128
display id: range2382128
version: 1
top level: BBa_K1486007
direction: direction/forward
start: 1
end: 3
orientation: inline

IFP[2]
type: SequenceAnnotation
persistent identity: annotation2382164
display id: annotation2382164
version: 1
top level: BBa_K1486007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382164/range2382164/1

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382164/range2382164/1
type: Range
persistent identity: range2382164
display id: range2382164
version: 1
top level: BBa_K1486007
direction: direction/forward
start: 1
end: 579
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2382163
display id: annotation2382163
version: 1
top level: BBa_K1486007
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382163/range2382163/1

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382163/range2382163/1
type: Range
persistent identity: range2382163
display id: range2382163
version: 1
top level: BBa_K1486007
direction: direction/forward
start: 577
end: 579
orientation: inline

TtrpC
type: ComponentDefinition
persistent identity: BBa K678036
display id: BBa_K678036
version: 1
was generated by: iGEM to SBOL conversion
description: trpC terminator for Aspergillus nidulans
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: From the plasmid p71 from the Center for Microbial Biotechnology at the Technical University of Denmark plasmid collection.
owned by: james
owned by: myers
mutable description: TtrpC is a terminator of tryptophan synthase of Aspergillus nidulans.
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: TtrpC terminator
sequence: https://synbiohub.org/public/igem/BBa_K678036_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127403
display id: annotation2127403
version: 1
top level: TtrpC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678036/annotation2127403/range2127403/1

https://synbiohub.org/public/igem/BBa_K678036/annotation2127403/range2127403/1
type: Range
persistent identity: range2127403
display id: range2127403
version: 1
top level: TtrpC
direction: direction/forward
start: 1
end: 8
orientation: inline

TtrpC terminator
type: SequenceAnnotation
persistent identity: annotation2127404
display id: annotation2127404
version: 1
top level: TtrpC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678036/annotation2127404/range2127404/1

https://synbiohub.org/public/igem/BBa_K678036/annotation2127404/range2127404/1
type: Range
persistent identity: range2127404
display id: range2127404
version: 1
top level: TtrpC
direction: direction/forward
start: 9
end: 759
orientation: inline

BBa_I746916
type: ComponentDefinition
persistent identity: BBa I746916
display id: BBa_I746916
version: 1
was generated by: iGEM to SBOL conversion
description: superfolder GFP coding sequence
created: 2008-09-29T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006 This version was synthesised de novo (by Geneart).
owned by: james
owned by: myers
mutable description: This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). It carries the following amino acid changes with respect to mut3 GFP (E0040), the currently most commonly used GFP in the registry: S30R, Y39N, F64L, G65T, F99S, N105T, Y145F, M153T, V163A, I171V, A206V Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP. Note: Superfolder GFP is available in constructs driven by the pBAD and T7 promoters: part numbers I746908 and I746909 respectively. Additionally 6-his tagged versions for protein purification exist: I746914 (pBAD driven) and I746915 (T7 driven).
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Codon optimisation before de novo synthesis was carried out for both, E.coli and Bacillus subtilis.
star: false
creator: Stefan Milde
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: superfolder GFP coding region
sequence: https://synbiohub.org/public/igem/BBa_I746916_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1977535
display id: annotation1977535
version: 1
top level: BBa_I746916
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977535/range1977535/1

https://synbiohub.org/public/igem/BBa_I746916/annotation1977535/range1977535/1
type: Range
persistent identity: range1977535
display id: range1977535
version: 1
top level: BBa_I746916
direction: direction/forward
start: 715
end: 720
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1977533
display id: annotation1977533
version: 1
top level: BBa_I746916
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977533/range1977533/1

https://synbiohub.org/public/igem/BBa_I746916/annotation1977533/range1977533/1
type: Range
persistent identity: range1977533
display id: range1977533
version: 1
top level: BBa_I746916
direction: direction/forward
start: 1
end: 3
orientation: inline

superfolder GFP coding region
type: SequenceAnnotation
persistent identity: annotation1977534
display id: annotation1977534
version: 1
top level: BBa_I746916
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977534/range1977534/1

https://synbiohub.org/public/igem/BBa_I746916/annotation1977534/range1977534/1
type: Range
persistent identity: range1977534
display id: range1977534
version: 1
top level: BBa_I746916
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B1007
type: ComponentDefinition
persistent identity: BBa B1007
display id: BBa_B1007
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~=80)
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~=80% # 8bp stem, 6nt loop # Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 6 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: modified thr
sequence annotation: B1007
sequence: https://synbiohub.org/public/igem/BBa_B1007_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1900957
display id: annotation1900957
version: 1
top level: BBa_B1007
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900957/range1900957/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900957/range1900957/1
type: Range
persistent identity: range1900957
display id: range1900957
version: 1
top level: BBa_B1007
direction: direction/reverse
start: 4
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1900958
display id: annotation1900958
version: 1
top level: BBa_B1007
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900958/range1900958/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900958/range1900958/1
type: Range
persistent identity: range1900958
display id: range1900958
version: 1
top level: BBa_B1007
direction: direction/forward
start: 32
end: 37
orientation: inline

modified thr
type: SequenceAnnotation
persistent identity: annotation1900956
display id: annotation1900956
version: 1
top level: BBa_B1007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900956/range1900956/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900956/range1900956/1
type: Range
persistent identity: range1900956
display id: range1900956
version: 1
top level: BBa_B1007
direction: direction/forward
start: 10
end: 31
orientation: inline

B1007
type: SequenceAnnotation
persistent identity: annotation1900955
display id: annotation1900955
version: 1
top level: BBa_B1007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900955/range1900955/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900955/range1900955/1
type: Range
persistent identity: range1900955
display id: range1900955
version: 1
top level: BBa_B1007
direction: direction/forward
start: 1
end: 40
orientation: inline

BBa_K1031941
type: ComponentDefinition
persistent identity: BBa K1031941
display id: BBa_K1031941
version: 1
was generated by: iGEM to SBOL conversion
description: Pm-B0034-eGFP (XylS)
created: 2013-10-20T11:00:00Z
modified: 2015-05-08T01:08:48Z
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
mutable description: Pm-B0034-eGFP (XylS)
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0015
sequence annotation: B0034
sequence annotation: eGFP
sequence annotation: Pm
sequence: https://synbiohub.org/public/igem/BBa_K1031941_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2370035
display id: annotation2370035
version: 1
top level: BBa_K1031941
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370035/range2370035/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370035/range2370035/1
type: Range
persistent identity: range2370035
display id: range2370035
version: 1
top level: BBa_K1031941
direction: direction/forward
start: 984
end: 1112
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2370033
display id: annotation2370033
version: 1
top level: BBa_K1031941
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370033/range2370033/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370033/range2370033/1
type: Range
persistent identity: range2370033
display id: range2370033
version: 1
top level: BBa_K1031941
direction: direction/forward
start: 89
end: 101
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2370034
display id: annotation2370034
version: 1
top level: BBa_K1031941
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370034/range2370034/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370034/range2370034/1
type: Range
persistent identity: range2370034
display id: range2370034
version: 1
top level: BBa_K1031941
direction: direction/forward
start: 107
end: 977
orientation: inline

Pm
type: SequenceAnnotation
persistent identity: annotation2370032
display id: annotation2370032
version: 1
top level: BBa_K1031941
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370032/range2370032/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370032/range2370032/1
type: Range
persistent identity: range2370032
display id: range2370032
version: 1
top level: BBa_K1031941
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_K780000
type: ComponentDefinition
persistent identity: BBa K780000
display id: BBa_K780000
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator for Bacillus subtilis
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
mutable provenance: Terminator of gyrase (EC 5.99.1.3) gene.
owned by: james
owned by: myers
mutable description: Our terminator is based on strong, Rho-independent terminator of gyrase gene, which is type II topoisomerase (EC 5.99.1.3). DNA gyrase uses the energy of ATP hydrolysis to introduce negative supercoils or relaxes positive supercoils. Negative DNA supercoiling is essential in vivo to compact the genome, relieve torsional strain during replication, and promote local melting for vital processes such as transcript initation by RNA polymerase. Gyrase gene is an housekeeping gene and because of that it must have efficient terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Nikodem Latocha
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K780000_sequence/1

BBa_K678038
type: ComponentDefinition
persistent identity: BBa K678038
display id: BBa_K678038
version: 1
was generated by: iGEM to SBOL conversion
description: T2-motni, terminator for Aspergillus niger
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: Aspergillus niger genomic DNA
owned by: james
owned by: myers
mutable description: T2-motni is a terminator for use in Aspergillus niger
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: T2-motni terminator
sequence: https://synbiohub.org/public/igem/BBa_K678038_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127407
display id: annotation2127407
version: 1
top level: BBa_K678038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678038/annotation2127407/range2127407/1

https://synbiohub.org/public/igem/BBa_K678038/annotation2127407/range2127407/1
type: Range
persistent identity: range2127407
display id: range2127407
version: 1
top level: BBa_K678038
direction: direction/forward
start: 1
end: 8
orientation: inline

T2-motni terminator
type: SequenceAnnotation
persistent identity: annotation2127408
display id: annotation2127408
version: 1
top level: BBa_K678038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678038/annotation2127408/range2127408/1

https://synbiohub.org/public/igem/BBa_K678038/annotation2127408/range2127408/1
type: Range
persistent identity: range2127408
display id: range2127408
version: 1
top level: BBa_K678038
direction: direction/forward
start: 9
end: 990
orientation: inline

BBa_J61048
type: ComponentDefinition
persistent identity: BBa J61048
display id: BBa_J61048
version: 1
was generated by: iGEM to SBOL conversion
description: [rnpB-T1] Terminator
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_J61048_sequence/1

BBa_K1031421
type: ComponentDefinition
persistent identity: BBa K1031421
display id: BBa_K1031421
version: 1
was generated by: iGEM to SBOL conversion
description: Ph-B0032-sfGFP-Terminator (HcaR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis, lab made
owned by: myers
owned by: james
mutable description: Ph-32-sfGFP-TT (HcaR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Ph (HcaR)
sequence annotation: sfGFP
sequence annotation: B0015
sequence annotation: B0032
sequence: https://synbiohub.org/public/igem/BBa_K1031421_sequence/1

Ph (HcaR)
type: SequenceAnnotation
persistent identity: annotation2362372
display id: annotation2362372
version: 1
top level: BBa_K1031421
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362372/range2362372/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362372/range2362372/1
type: Range
persistent identity: range2362372
display id: range2362372
version: 1
top level: BBa_K1031421
direction: direction/forward
start: 1
end: 74
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362385
display id: annotation2362385
version: 1
top level: BBa_K1031421
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362385/range2362385/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362385/range2362385/1
type: Range
persistent identity: range2362385
display id: range2362385
version: 1
top level: BBa_K1031421
direction: direction/forward
start: 100
end: 822
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362386
display id: annotation2362386
version: 1
top level: BBa_K1031421
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362386/range2362386/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362386/range2362386/1
type: Range
persistent identity: range2362386
display id: range2362386
version: 1
top level: BBa_K1031421
direction: direction/forward
start: 829
end: 957
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2362373
display id: annotation2362373
version: 1
top level: BBa_K1031421
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362373/range2362373/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362373/range2362373/1
type: Range
persistent identity: range2362373
display id: range2362373
version: 1
top level: BBa_K1031421
direction: direction/forward
start: 81
end: 93
orientation: inline

BBa_K1031302
type: ComponentDefinition
persistent identity: BBa K1031302
display id: BBa_K1031302
version: 1
was generated by: iGEM to SBOL conversion
description: Pc-B0032-sfGFP-Terminator (HbpR)
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
mutable description: Ph-32-sfGFP-TT (HbpR)
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_B0032
sequence annotation: BBa_B0015
sequence annotation: Ph
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031302_sequence/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2360437
display id: annotation2360437
version: 1
top level: BBa_K1031302
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360437/range2360437/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360437/range2360437/1
type: Range
persistent identity: range2360437
display id: range2360437
version: 1
top level: BBa_K1031302
direction: direction/forward
start: 236
end: 247
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2360439
display id: annotation2360439
version: 1
top level: BBa_K1031302
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360439/range2360439/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360439/range2360439/1
type: Range
persistent identity: range2360439
display id: range2360439
version: 1
top level: BBa_K1031302
direction: direction/forward
start: 983
end: 1111
orientation: inline

Ph
type: SequenceAnnotation
persistent identity: annotation2360436
display id: annotation2360436
version: 1
top level: BBa_K1031302
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360436/range2360436/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360436/range2360436/1
type: Range
persistent identity: range2360436
display id: range2360436
version: 1
top level: BBa_K1031302
direction: direction/forward
start: 1
end: 229
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2360438
display id: annotation2360438
version: 1
top level: BBa_K1031302
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360438/range2360438/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360438/range2360438/1
type: Range
persistent identity: range2360438
display id: range2360438
version: 1
top level: BBa_K1031302
direction: direction/forward
start: 254
end: 976
orientation: inline

BBa_B1002
type: ComponentDefinition
persistent identity: BBa B1002
display id: BBa_B1002
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, small, %T~=85%)
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
mutable description: Artifical terminator, estimated %T~=85
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Poly A tail
sequence annotation: stem loop
sequence annotation: B1002
sequence: https://synbiohub.org/public/igem/BBa_B1002_sequence/1

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898414
display id: annotation1898414
version: 1
top level: BBa_B1002
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
type: Range
persistent identity: range1898414
display id: range1898414
version: 1
top level: BBa_B1002
direction: direction/forward
start: 25
end: 31
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898415
display id: annotation1898415
version: 1
top level: BBa_B1002
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
type: Range
persistent identity: range1898415
display id: range1898415
version: 1
top level: BBa_B1002
direction: direction/reverse
start: 4
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898413
display id: annotation1898413
version: 1
top level: BBa_B1002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
type: Range
persistent identity: range1898413
display id: range1898413
version: 1
top level: BBa_B1002
direction: direction/forward
start: 10
end: 25
orientation: inline

B1002
type: SequenceAnnotation
persistent identity: annotation1898412
display id: annotation1898412
version: 1
top level: BBa_B1002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
type: Range
persistent identity: range1898412
display id: range1898412
version: 1
top level: BBa_B1002
direction: direction/forward
start: 1
end: 34
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem loop
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: iGEM to SBOL conversion
description: -- No description --
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to bacteriophage T3
sequence annotation: stem loop from bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 28
end: 66
orientation: inline

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 36
end: 57
orientation: inline

BBa_K1031422
type: ComponentDefinition
persistent identity: BBa K1031422
display id: BBa_K1031422
version: 1
was generated by: iGEM to SBOL conversion
description: Ph(HcaR)-B0034-sfGFP
created: 2013-10-17T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: E.coli
owned by: james
owned by: myers
mutable description: Ph(HcaR)-B0034-sfGFP
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: B0015
sequence annotation: B0034
sequence annotation: Ph (HcaR)
sequence: https://synbiohub.org/public/igem/BBa_K1031422_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2369614
display id: annotation2369614
version: 1
top level: BBa_K1031422
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369614/range2369614/1

https://synbiohub.org/public/igem/BBa_K1031422/annotation2369614/range2369614/1
type: Range
persistent identity: range2369614
display id: range2369614
version: 1
top level: BBa_K1031422
direction: direction/forward
start: 101
end: 823
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2369616
display id: annotation2369616
version: 1
top level: BBa_K1031422
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369616/range2369616/1

https://synbiohub.org/public/igem/BBa_K1031422/annotation2369616/range2369616/1
type: Range
persistent identity: range2369616
display id: range2369616
version: 1
top level: BBa_K1031422
direction: direction/forward
start: 830
end: 958
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2369615
display id: annotation2369615
version: 1
top level: BBa_K1031422
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369615/range2369615/1

https://synbiohub.org/public/igem/BBa_K1031422/annotation2369615/range2369615/1
type: Range
persistent identity: range2369615
display id: range2369615
version: 1
top level: BBa_K1031422
direction: direction/forward
start: 84
end: 95
orientation: inline

Ph (HcaR)
type: SequenceAnnotation
persistent identity: annotation2369613
display id: annotation2369613
version: 1
top level: BBa_K1031422
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369613/range2369613/1

https://synbiohub.org/public/igem/BBa_K1031422/annotation2369613/range2369613/1
type: Range
persistent identity: range2369613
display id: range2369613
version: 1
top level: BBa_K1031422
direction: direction/forward
start: 1
end: 74
orientation: inline

BBa_K259006
type: ComponentDefinition
persistent identity: BBa K259006
display id: BBa_K259006
version: 1
was generated by: iGEM to SBOL conversion
description: GFP-Terminator
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: GFP originates from jellyfish Aequeora victoria. For more details visit the [http://www.expasy.ch/uniprot/P42212 SwissProt entry]. The double terminator was designed by Reshma Shetty. For more details visis the [http://partsregistry.org/Part:BBa_B0014 original part page].
owned by: james
owned by: myers
mutable description: ==Part Description== This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator. Green Fluorescent protein (originally from part [http://partsregistry.org/Part:BBa_E0040 BBa_E0040]) that will glow green when excited by the correct wavelengths and thus can be used as a reporter gene. This part is directly fused to a terminator (originally from part [http://partsregistry.org/Part:BBa_B0014 BBa_B0014]) so it is ideal to be fused at the end of your construct. ==Table of Statistics== {| style="color:white; background-color:#0066CC;" cellpadding="20" cellspacing="0" border="2" !Chassis !Monomer/Multimer !Aminoacid Length/Weight !Localisation !Structure !Excitation wavelengths !Emmision wavelengths |- |Prokaryotic and Eukaryotic |Bipartite |274 aminoacids | |beta-barrel(for GFP) hairpins for Terminator |501nm |511nm |} ==References== #Valdivia pmid=8707053
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: No additional design considerations. The parts were fused together following RFC10 assembly.
star: false
creator: Petros Mina
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0011
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_K259006_sequence/1

BBa_B0011
type: Component
persistent identity: component2020534
display id: component2020534
version: 1
top level: BBa_K259006
access: public
definition: BBa_B0011

BBa_E0040
type: Component
persistent identity: component2020529
display id: component2020529
version: 1
top level: BBa_K259006
access: public
definition: GFP

BBa_B0012
type: Component
persistent identity: component2020530
display id: component2020530
version: 1
top level: BBa_K259006
access: public
definition: BBa_B0012

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2020529
display id: annotation2020529
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020529/range2020529/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K259006/annotation2020529/range2020529/1
type: Range
persistent identity: range2020529
display id: range2020529
version: 1
top level: BBa_K259006
direction: direction/reverse
start: 1
end: 720
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2020530
display id: annotation2020530
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020530/range2020530/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K259006/annotation2020530/range2020530/1
type: Range
persistent identity: range2020530
display id: range2020530
version: 1
top level: BBa_K259006
direction: direction/forward
start: 729
end: 769
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2020534
display id: annotation2020534
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020534/range2020534/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K259006/annotation2020534/range2020534/1
type: Range
persistent identity: range2020534
display id: range2020534
version: 1
top level: BBa_K259006
direction: direction/forward
start: 778
end: 823
orientation: inline

BBa_K678039
type: ComponentDefinition
persistent identity: BBa K678039
display id: BBa_K678039
version: 1
was generated by: iGEM to SBOL conversion
description: T3-motni, terminator for Aspergillus niger
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: Aspergillus niger genomic DNA
owned by: james
owned by: myers
mutable description: T3-motni is a terminator for use in Aspergillus niger.
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: T3-motni terminator
sequence: https://synbiohub.org/public/igem/BBa_K678039_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127409
display id: annotation2127409
version: 1
top level: BBa_K678039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678039/annotation2127409/range2127409/1

https://synbiohub.org/public/igem/BBa_K678039/annotation2127409/range2127409/1
type: Range
persistent identity: range2127409
display id: range2127409
version: 1
top level: BBa_K678039
direction: direction/forward
start: 1
end: 8
orientation: inline

T3-motni terminator
type: SequenceAnnotation
persistent identity: annotation2127410
display id: annotation2127410
version: 1
top level: BBa_K678039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678039/annotation2127410/range2127410/1

https://synbiohub.org/public/igem/BBa_K678039/annotation2127410/range2127410/1
type: Range
persistent identity: range2127410
display id: range2127410
version: 1
top level: BBa_K678039
direction: direction/forward
start: 9
end: 889
orientation: inline

BBa_K1468000
type: ComponentDefinition
persistent identity: BBa K1468000
display id: BBa_K1468000
version: 1
was generated by: iGEM to SBOL conversion
description: pJ23104 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: ZsGreen protein
sequence annotation: BBa_K1468000
sequence annotation: BBa_J23104
sequence annotation: STOP
sequence annotation: ZsGreeen
sequence annotation: START
sequence annotation: J23104p
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K1468000_sequence/1

ZsGreen protein
type: SequenceAnnotation
persistent identity: annotation2418059
display id: annotation2418059
version: 1
top level: BBa_K1468000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1
type: Range
persistent identity: range2418059
display id: range2418059
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

BBa_K1468000
type: SequenceAnnotation
persistent identity: annotation2418810
display id: annotation2418810
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1
type: Range
persistent identity: range2418810
display id: range2418810
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 725
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2418058
display id: annotation2418058
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1
type: Range
persistent identity: range2418058
display id: range2418058
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2418064
display id: annotation2418064
version: 1
top level: BBa_K1468000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1
type: Range
persistent identity: range2418064
display id: range2418064
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 725
end: 725
orientation: inline

ZsGreeen
type: SequenceAnnotation
persistent identity: annotation2418062
display id: annotation2418062
version: 1
top level: BBa_K1468000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1
type: Range
persistent identity: range2418062
display id: range2418062
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2418061
display id: annotation2418061
version: 1
top level: BBa_K1468000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1
type: Range
persistent identity: range2418061
display id: range2418061
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 36
orientation: inline

J23104p
type: SequenceAnnotation
persistent identity: annotation2418030
display id: annotation2418030
version: 1
top level: BBa_K1468000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1
type: Range
persistent identity: range2418030
display id: range2418030
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2418060
display id: annotation2418060
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1
type: Range
persistent identity: range2418060
display id: range2418060
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

BBa_K864601
type: ComponentDefinition
persistent identity: BBa K864601
display id: BBa_K864601
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda t1 transcriptional terminator
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
mutable provenance: Gene synthesis
owned by: james
owned by: myers
mutable description: The lambda t1 is a strong transcriptional terminator with 99% efficiency in vivo.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Erik Lundin
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: lambda t1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K864601_sequence/1

lambda t1 terminator
type: SequenceAnnotation
persistent identity: annotation2197328
display id: annotation2197328
version: 1
top level: BBa_K864601
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864601/annotation2197328/range2197328/1

https://synbiohub.org/public/igem/BBa_K864601/annotation2197328/range2197328/1
type: Range
persistent identity: range2197328
display id: range2197328
version: 1
top level: BBa_K864601
direction: direction/forward
start: 1
end: 53
orientation: inline

BBa_K104007
type: ComponentDefinition
persistent identity: BBa K104007
display id: BBa_K104007
version: 1
was generated by: iGEM to SBOL conversion
description: Term_rrnO
creator: Anil Wipat
created: 2009-10-13T11:00:00Z
modified: 2015-06-09T08:51:00Z
discontinued: false
dominant: false
group u list: _237_
m user id: 4206
owner id: 2652
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is an efficient termintor sequence from Bacillus that normally terminates the rrnO-16S-trnO-Ile-trnO-Ala-rrnO-23S-rrnO-5S operon.
mutable notes: This terminator forms part of the complex device BBa_K104001
mutable provenance: The sequence was derived from DBTBS http://dbtbs.hgc.jp/COG/prom/rrnO-16S-trnO-Ile-trnO-Ala-rrnO-23S-rrnO-5S.html
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K104007_sequence/1

BBa_K1031624
type: ComponentDefinition
persistent identity: BBa K1031624
display id: BBa_K1031624
version: 1
was generated by: iGEM to SBOL conversion
description: J23114-NahF-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
mutable description: 114-NahF-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: NahF
sequence annotation: J23114
sequence: https://synbiohub.org/public/igem/BBa_K1031624_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2361905
display id: annotation2361905
version: 1
top level: BBa_K1031624
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361905/range2361905/1

https://synbiohub.org/public/igem/BBa_K1031624/annotation2361905/range2361905/1
type: Range
persistent identity: range2361905
display id: range2361905
version: 1
top level: BBa_K1031624
direction: direction/forward
start: 1520
end: 1648
orientation: inline

NahF
type: SequenceAnnotation
persistent identity: annotation2361904
display id: annotation2361904
version: 1
top level: BBa_K1031624
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361904/range2361904/1

https://synbiohub.org/public/igem/BBa_K1031624/annotation2361904/range2361904/1
type: Range
persistent identity: range2361904
display id: range2361904
version: 1
top level: BBa_K1031624
direction: direction/forward
start: 62
end: 1513
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2361903
display id: annotation2361903
version: 1
top level: BBa_K1031624
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361903/range2361903/1

https://synbiohub.org/public/igem/BBa_K1031624/annotation2361903/range2361903/1
type: Range
persistent identity: range2361903
display id: range2361903
version: 1
top level: BBa_K1031624
direction: direction/forward
start: 1
end: 35
orientation: inline

BBa_K1462070
type: ComponentDefinition
persistent identity: BBa K1462070
display id: BBa_K1462070
version: 1
was generated by: iGEM to SBOL conversion
description: cyc1
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1462070_sequence/1

BBa_K678012
type: ComponentDefinition
persistent identity: BBa K678012
display id: BBa_K678012
version: 1
was generated by: iGEM to SBOL conversion
description: SV40 poly A, terminator for mammalian cells
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
mutable description: SV40 poly A, terminator for use in mammalian cells
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: SV40
sequence annotation: Linker 4
sequence: https://synbiohub.org/public/igem/BBa_K678012_sequence/1

SV40
type: SequenceAnnotation
persistent identity: annotation2127355
display id: annotation2127355
version: 1
top level: BBa_K678012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678012/annotation2127355/range2127355/1

https://synbiohub.org/public/igem/BBa_K678012/annotation2127355/range2127355/1
type: Range
persistent identity: range2127355
display id: range2127355
version: 1
top level: BBa_K678012
direction: direction/forward
start: 9
end: 139
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127354
display id: annotation2127354
version: 1
top level: BBa_K678012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678012/annotation2127354/range2127354/1

https://synbiohub.org/public/igem/BBa_K678012/annotation2127354/range2127354/1
type: Range
persistent identity: range2127354
display id: range2127354
version: 1
top level: BBa_K678012
direction: direction/forward
start: 1
end: 8
orientation: inline

BBa_K392003
type: ComponentDefinition
persistent identity: BBa K392003
display id: BBa_K392003
version: 1
was generated by: iGEM to SBOL conversion
description: yeast ADH1 terminator
created: 2010-10-09T11:00:00Z
modified: 2015-05-08T01:12:20Z
mutable provenance: Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak).
owned by: james
owned by: myers
mutable description: Engineered 'cyc100 promoter' of Saccharomyces cerevisiae with Kozak sequence attached downstream.
discontinued: false
dominant: false
experience: experience/Works
group u list: _528_
m user id: 0
owner id: 6220
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Assembled by 3A method.
star: false
creator: Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K392003_sequence/1

BBa_K1031100
type: ComponentDefinition
persistent identity: BBa K1031100
display id: BBa_K1031100
version: 1
was generated by: iGEM to SBOL conversion
description: CapR-Terminator
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: synthesis
owned by: james
owned by: myers
mutable description: CapR-TT
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Pseudomonas putida
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CapR
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031100_sequence/1

CapR
type: SequenceAnnotation
persistent identity: annotation2361063
display id: annotation2361063
version: 1
top level: BBa_K1031100
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031100/annotation2361063/range2361063/1

https://synbiohub.org/public/igem/BBa_K1031100/annotation2361063/range2361063/1
type: Range
persistent identity: range2361063
display id: range2361063
version: 1
top level: BBa_K1031100
direction: direction/forward
start: 1
end: 1692
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361122
display id: annotation2361122
version: 1
top level: BBa_K1031100
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031100/annotation2361122/range2361122/1

https://synbiohub.org/public/igem/BBa_K1031100/annotation2361122/range2361122/1
type: Range
persistent identity: range2361122
display id: range2361122
version: 1
top level: BBa_K1031100
direction: direction/forward
start: 1699
end: 1827
orientation: inline

BBa_K1031311
type: ComponentDefinition
persistent identity: BBa K1031311
display id: BBa_K1031311
version: 1
was generated by: iGEM to SBOL conversion
description: J23114-HbpR-Terminator
created: 2013-10-12T11:00:00Z
modified: 2015-05-08T01:08:47Z
mutable provenance: P.putida
owned by: james
owned by: myers
mutable description: 114-HbpR-TT
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: molecular cloning
star: false
creator: He Shuaixin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: HbpR
sequence annotation: J23114
sequence: https://synbiohub.org/public/igem/BBa_K1031311_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2369599
display id: annotation2369599
version: 1
top level: BBa_K1031311
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369599/range2369599/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369599/range2369599/1
type: Range
persistent identity: range2369599
display id: range2369599
version: 1
top level: BBa_K1031311
direction: direction/forward
start: 1782
end: 1910
orientation: inline

HbpR
type: SequenceAnnotation
persistent identity: annotation2369598
display id: annotation2369598
version: 1
top level: BBa_K1031311
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369598/range2369598/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369598/range2369598/1
type: Range
persistent identity: range2369598
display id: range2369598
version: 1
top level: BBa_K1031311
direction: direction/forward
start: 63
end: 1713
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2369597
display id: annotation2369597
version: 1
top level: BBa_K1031311
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369597/range2369597/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369597/range2369597/1
type: Range
persistent identity: range2369597
display id: range2369597
version: 1
top level: BBa_K1031311
direction: direction/forward
start: 1
end: 35
orientation: inline

BBa_K1486004
type: ComponentDefinition
persistent identity: BBa K1486004
display id: BBa_K1486004
version: 1
was generated by: iGEM to SBOL conversion
description: flexible linker 2x (GGGGS)
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: Flexible linker used to fuse two proteins.
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Designed considering the flexibility and separation needed between our fused proteins.
star: false
creator: iGem EPFL 2014
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1486004_sequence/1

BBa_K801011
type: ComponentDefinition
persistent identity: BBa K801011
display id: BBa_K801011
version: 1
was generated by: iGEM to SBOL conversion
description: TEF1 yeast terminator
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
mutable description: TEF1 terminator of ''S. cervisiae''.
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.
star: false
creator: Georg Sch??tzinger
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: TEF1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801011_sequence/1

TEF1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202142
display id: annotation2202142
version: 1
top level: BBa_K801011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801011/annotation2202142/range2202142/1

https://synbiohub.org/public/igem/BBa_K801011/annotation2202142/range2202142/1
type: Range
persistent identity: range2202142
display id: range2202142
version: 1
top level: BBa_K801011
direction: direction/forward
start: 1
end: 507
orientation: inline

BBa_B0050
type: ComponentDefinition
persistent identity: BBa B0050
display id: BBa_B0050
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (pBR322, +/-)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Bi-directional Terminator derived from pBR322.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0050_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318591
display id: annotation318591
version: 1
top level: BBa_B0050
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0050/annotation318591/range318591/1

https://synbiohub.org/public/igem/BBa_B0050/annotation318591/range318591/1
type: Range
persistent identity: range318591
display id: range318591
version: 1
top level: BBa_B0050
direction: direction/forward
start: 8
end: 24
orientation: inline

BBa_B0022
type: ComponentDefinition
persistent identity: BBa B0022
display id: BBa_B0022
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7, reversed
created: 2003-10-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Uni-directional terminator. Reverse of part B0012. 20 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0022_sequence/1

BBa_K2110000
type: ComponentDefinition
persistent identity: BBa K2110000
display id: BBa_K2110000
version: 1
was generated by: iGEM to SBOL conversion
description: CYC1 Terminator
creator: Haoran Ding
created: 2016-10-03T11:00:00Z
modified: 2016-10-04T10:55:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2578_
m user id: 32964
owner id: 32964
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CYC1 Terminator is widely used in Saccharomyces cerevisiae. It is a composite part in many vectors of Saccharomyces cerevisiae like plasmid pYES2. Amplifying it by PCR and link it after your protein coding sequence is also feasible if you want to express the gene in Saccharomyces cerevisiae.
mutable notes: There is no neccessary consideration in detailed design of the sequence
mutable provenance: It comes from genome sequence of Saccharomyces cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: CYC1 Terminator
sequence: https://synbiohub.org/public/igem/BBa_K2110000_sequence/1

CYC1 Terminator
type: SequenceAnnotation
persistent identity: annotation2485997
display id: annotation2485997
version: 1
top level: BBa_K2110000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2110000/annotation2485997/range2485997/1

https://synbiohub.org/public/igem/BBa_K2110000/annotation2485997/range2485997/1
type: Range
persistent identity: range2485997
display id: range2485997
version: 1
direction: direction/forward
top level: BBa_K2110000
start: 1
end: 1
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K1031302_sequence/1
type: Sequence
persistent identity: BBa K1031302 sequence
display id: BBa_K1031302_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttaataaatttatgaaatcgtggttgtgagttttcataatatggtgaagcttgcccgccatggcaagtgcatttcggcagctgttggcgaccgcggaaggggtttacagccgtctggagctaggctttctggcgctcattaaaataaaaatccttataaaacagtatcctagcttttatgtctgaggctgcttagtcaacctggcacggtactggctacgagtcccgctactagcacacaggaaagtactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataatactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031114_sequence/1
type: Sequence
persistent identity: BBa K1031114 sequence
display id: BBa_K1031114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcatttgctcaagcggccttgggcaattgatcaaatgcttaaaaagtctgcgcaagcgcggcttaatttcgctcgctccgatcattctaaaaattagaaacacattgaaaaacattaccttgaagtctgttttcagaccttggcacagccgttgcttgatgtcctgcactagatcacacaggaaagtactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I51003_sequence/1
type: Sequence
persistent identity: BBa I51003 sequence
display id: BBa_I51003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatcatgtagctagcgtgccgtagctagtcgctgactgatgctgactgactgcgactgactgactggcgctagctgcatgcatgctgactgactgactgatcggcgatcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J63002_sequence/1
type: Sequence
persistent identity: BBa J63002 sequence
display id: BBa_J63002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1002_sequence/1
type: Sequence
persistent identity: BBa B1002 sequence
display id: BBa_B1002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0060_sequence/1
type: Sequence
persistent identity: BBa B0060 sequence
display id: BBa_B0060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaatcaaaggatcttcttgagatccttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1007_sequence/1
type: Sequence
persistent identity: BBa B1007 sequence
display id: BBa_B1007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcaaaaaaccccgcccctgacagggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678018_sequence/1
type: Sequence
persistent identity: BBa K678018 sequence
display id: BBa_K678018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attccgataggatcccgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttaaaataactataccagcaggaggacgtccagacacagcataggctacctggccatgcccaaccggtgggacatttgagttgcttgcttggcactgtcctctcatgcgttgggtccactcagtagatgcctgttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031916_sequence/1
type: Sequence
persistent identity: BBa K1031916 sequence
display id: BBa_K1031916_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatggatttttgcttattgaacgagaaaagtcagatcttcgtccacgccgagccctatgcagtctccgattatgttaaccagtatgtcggtacgcactctattcgcctgcccaagggcgggcgcccggcaggcaggctgcaccacagaatcttcggatgcctcgacctgtgtcgaatcagctacggcggtagcgtgagggtaatctcgcctggattagagacctgttatcatctgcaaataatactcaaaggccattgcctgtggcgtggccatggccaggagcactattttgcgccgggcgaactattgctgctcaatccggatgaccaagccgacctgacctattcagaagattgcgagaaatttatcgttaaattgccctcagtggtccttgatcgggcatgcagtgacaacaattggcacaagccgagggagggtatccgtttcgccgcgcgacacaatctccagcaactcgatggctttatcaatctactcgggttagtttgtgacgaagcggaacatacaaagtcgatgcctcgggtccaagagcactatgcggggatcatcgcttccaagctgctcgaaatgctgggcagcaatgtcagccgtgaaattttcagcaaaggtaacccgtctttcgagcgagtcgttcaattcattgaggagaatctcaaacggaatatcagccttgagcggttagcggagctggcgatgatgagtccacgctcgctctacaatttgttcgagaagcatgccggcaccacgccgaagaactacatccgcaaccgcaagctcgaaagcatccgcgcctgcttgaacgatcccagtgccaatgtgcgtagtataactgagatagccctagactacggcttcttacatttgggacgcttcgctgaaaactataggagcgcgttcggcgagttgccttccgacaccctgcgtcaatgcaaaaaggaagtggcttgaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1840002_sequence/1
type: Sequence
persistent identity: BBa K1840002 sequence
display id: BBa_K1840002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486005_sequence/1
type: Sequence
persistent identity: BBa K1486005 sequence
display id: BBa_K1486005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcaaagaggagaaaatgaataaaatcctgttagttgatgatgaccgagagctgacttccctattaaaggagctgctcgagatggaaggcttcaacgtgattgttgcccacgatggggaacaggcgcttgatcttctggacgacagcattgatttacttttgcttgacgtaatgatgccgaagaaaaatggtatcgacacattaaaagcacttcgccagacacaccagacgcctgtcattatgttgacggcgcgcggcagtgaacttgatcgcgttctcggccttgagctgggcgcagatgactatctcccgaaaccgtttaatgatcgtgagctggtggcacgtattcgcgcgatcctgcgccgttcgcactggagcgagcaacagcaaaacaacgacaacggttcaccgacactggaagttgatgccttagtgctgaatccaggccgtcaggaagccagcttcgacgggcaaacgctggagttaaccggtactgagtttaccctgctctatttgctggcacagcatctgggtcaggtggtttcccgtgaacatttaagccaggaagtgttgggcaaacgcctgacgcctttcgaccgcgctattgatatgcacatttccaacctgcgtcgtaaactgccggatcgtaaagatggtcacccgtggtttaaaaccttgcgtggtcgcggctatctgatggtttctgcttcataataaggtggtggttctggtggtggttctatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678036_sequence/1
type: Sequence
persistent identity: BBa K678036 sequence
display id: BBa_K678036_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attccgatggatccacttaacgttactgaaatcatcaaacagcttgacgaatctggatataagatcgttggtgtcgatgtcagctccggagttgagacaaatggtgttcaggatctcgataagatacgttcatttgtccaagcagcaaagagtgccttctagtgatttaatagctccatgtcaacaagaataaaacgcgttttcgggtttacctcttccagatacagctcatctgcaatgcattaatgcattgactgcaacctagtaacgcctttcaggctccggcgaagagaagaatagcttagcagagctattttcattttcgggagacgagatcaagcagatcaacggtcgtcaagagacctacgagactgaggaatccgctcttggctccacgcgactatatatttgtctctaattgtactttgacatgctcctcttctttactctgatagcttgactatgaaaattccgtcaccagctcctgggttcgcaaagataattgcatgtttcttccttgaactctcaagcctacaggacacacattcatcgtaggtataaacctcgaaatcatttcctactaagatggtatacaatagtaaccatgcatggttgcctagtgaatgctccgtaacacccaatacgccggccgaaacttttttacaactctcctatgagtcgtttacccagaatgcacaggtacacttgtttagaggtaatccttctttctagaagtcctcgtgtactgtgtaagcgccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031624_sequence/1
type: Sequence
persistent identity: BBa K1031624 sequence
display id: BBa_K1031624_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaagacaaaactgtttatcaataacgcctggatcgattctagtgaccagcagaccttcgagcgcatacaccccgtcagcagcgatgtggtgactgagagcgcaaacgccacagtgacggacgcgataaaggcggcgcaagcggccgaggaggcgttcaagacctggaaggccgttggaccttcagagcgtcgccgccttctcctaaaggtcgccgatgtcatggaaagtaaaacacccaagttcatcgaagtgatggccatggaggtgggagcttccgccctttgggccggattcaacgtccatgcgtctgccaatgtgttccgagaggctgcctcgctggctacccaaattcagggtgaaaccatcccaacggacaaagccgaaacgctctcaatgacactacgtcagccggtcggcccgatcctaagcatcgttccatggaacggcaccgcagtgcttgcggcacgagccatcgcttatccgctggtctgtggcaacactgtggtgttcaaaggctctgaatttagtcccgcgacgcatgccctgatcacccagtgcgtgcaggaagccgggctgcccgctggcgtgctcaattacctcaactcttcgcctgaccgttcgcccgagatcgctgacgcactgatctctgccaaggagatccgccgcatcaacttcacgggttccacccgcgtgggcagcattatcgcgcagaaagccgcgcaacacctcaagcgctgcctgctggagctcggcggcaagtccccgcttattgttctggatgatgcagacatcgatgcggcggtcaaggcagcggtgttcggtagcttcctgttccaaggtcagatctgcatgtccactgagcgcttgatcgttgatgagaagatagccgacgaatttgtcgcaaaatttgtcgaaaaaactaagcgcttgagcgcaggcgacccgtgcgtaactggcgactgcatcatcggcccgatggtctcgccaaattcgggtgagcggatcaatggtttgttcaaagacgcgatcgacaaaggggcaaaagttgtttgcggcggcttggcccaaggtgcgctcatgccggccacgatcctggatcacgtcaaatctgacatgcggatttacgatgaggagacctttggtcccatcaccgtggtaatccgttgtaaaggcgaagcagaggccgtccgcattgccaacgacagcgtctatggcctgtcgtcgggcgtatttggccgcgacatcaaccgcgctctacgcgtgggtatgtccatcgaatatggttctgtacacatcaacggttcgaccgcccagaacgaggcgcaggctccttacggaggcaccaagaacaccggctacgggcgcttcgacggccgtgctgtaatcgacgagttcacagagatcaagtggctgaccatcgaacctttcgagcagcaatatcccttctgaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0062_sequence/1
type: Sequence
persistent identity: BBa B0062 sequence
display id: BBa_B0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagataaaaaaaatccttagctttcgctaaggatgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J52016_sequence/1
type: Sequence
persistent identity: BBa J52016 sequence
display id: BBa_J52016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatatgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0023_sequence/1
type: Sequence
persistent identity: BBa B0023 sequence
display id: BBa_B0023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcaaaaaggcccacccgaaggtgagccagtttgatttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0450_sequence/1
type: Sequence
persistent identity: BBa C0450 sequence
display id: BBa_C0450_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaatattattactgagtaaaggattgttaccgcactaagcgggcaaaacctgaaaaaaattgcttgattcacgtcaggccgtttttttcaggtttttttttggagttttgccgcaaagcggtatactagaggaaatattattactgagtaaaggattgttaccgcactaagcgggcaaaacctgaaaaaaattgcttgattcacgtcaggccgtttttttcaggtttttttttggagttttgccgcaaagcggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0016_sequence/1
type: Sequence
persistent identity: BBa B0016 sequence
display id: BBa_B0016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0020_sequence/1
type: Sequence
persistent identity: BBa B0020 sequence
display id: BBa_B0020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1003_sequence/1
type: Sequence
persistent identity: BBa B1003 sequence
display id: BBa_B1003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgccaaaaaccccgcttcggcggggtttttccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031913_sequence/1
type: Sequence
persistent identity: BBa K1031913 sequence
display id: BBa_K1031913_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctagggactgtgctagctactagagaaagaggagaaatactagatggatttttgcttattgaacgagaaaagtcagatcttcgtccacgccgagccctatgcagtctccgattatgttaaccagtatgtcggtacgcactctattcgcctgcccaagggcgggcgcccggcaggcaggctgcaccacagaatcttcggatgcctcgacctgtgtcgaatcagctacggcggtagcgtgagggtaatctcgcctggattagagacctgttatcatctgcaaataatactcaaaggccattgcctgtggcgtggccatggccaggagcactattttgcgccgggcgaactattgctgctcaatccggatgaccaagccgacctgacctattcagaagattgcgagaaatttatcgttaaattgccctcagtggtccttgatcgggcatgcagtgacaacaattggcacaagccgagggagggtatccgtttcgccgcgcgacacaatctccagcaactcgatggctttatcaatctactcgggttagtttgtgacgaagcggaacatacaaagtcgatgcctcgggtccaagagcactatgcggggatcatcgcttccaagctgctcgaaatgctgggcagcaatgtcagccgtgaaattttcagcaaaggtaacccgtctttcgagcgagtcgttcaattcattgaggagaatctcaaacggaatatcagccttgagcggttagcggagctggcgatgatgagtccacgctcgctctacaatttgttcgagaagcatgccggcaccacgccgaagaactacatccgcaaccgcaagctcgaaagcatccgcgcctgcttgaacgatcccagtgccaatgtgcgtagtataactgagatagccctagactacggcttcttacatttgggacgcttcgctgaaaactataggagcgcgttcggcgagttgccttccgacaccctgcgtcaatgcaaaaaggaagtggcttgaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031222_sequence/1
type: Sequence
persistent identity: BBa K1031222 sequence
display id: BBa_K1031222_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcatttgctcaagcggccttgggcaattgatcaaatgcttaaaaagtctgcgcaagcgcggcttaatttcgctcgctccgatcattctaaaaattagaaacacattgaaaaacattaccttgaagtctgttttcagaccttggcacagccgttgcttgatgtcctgcgtactagagtcacacaggaaagactagaatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1005_sequence/1
type: Sequence
persistent identity: BBa B1005 sequence
display id: BBa_B1005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: cgccgcaaaccccgcttcggcggggtttcgccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031923_sequence/1
type: Sequence
persistent identity: BBa K1031923 sequence
display id: BBa_K1031923_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctagggactgtgctagcactagaattaaagaggagaaatactagatggaaatggaaatcaaagcagcaatagttcgccaaaaaaatggcccgttcttacttgagcatgtagctcttaatgagccagctgaagatcaggttctcgttagattggttgcaaccgggctgtgtcatacggatctggtttgtcgcgatcagcattatccggttccactaccgatggtatttgggcatgaaggggctggtgtggttgagcgggttgggtccgcggtcaaaaaggttcagccgggcgaccatgttgttttgacattttatacctgcgggagttgtgatgcttgtctttccggagaccctaccagttgtgcaaactcatttggccctaactttatggggcgctcggtaaccggggagtgcaccatccacgatcaccaaggggcagaggtgggagcaagcttttttgggcagtcctcctttgcgacatatgcgctatcttatgaacgtaacactgtgaaggttacaaaagacgtaccgcttgagttgcttgggcctcttggttgtggcattcaaactggcgcagggtctgttctgaatgcgcttaatccgccagcgggttctgctatcgcaatttttggtgctggggcagttggtctttcggccgtgatggctgccgttgtagcaggttgtaccaccatcatcgctgtcgacgttaaggaaaaccggctggaactagccagtgaacttggggcgacgcacattattaacccggccgctaacgatcccattgaggcgatcaaagagatattcgctgacggtgttccgtatgtattggagactagcggtttgcccgccgtgcttacgcaggcgatcctcagctctgctataggcggtgagatcggtattgtaggggcgccacctatgggggccacggtgcccgttgacattaacttcctgctattcaatcgtaagcttcgtggaatcgttgagggtcagtcgatctcggatattttcattcccaggctggtggagctttatcgccaggggaagtttccgtttgacaagctgattaagttttatccttttgatgaaatcaatcgagccgccgaagattcggaaaaaggcgtgacgcttaagccggtactccggattggttgaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0050_sequence/1
type: Sequence
persistent identity: BBa B0050 sequence
display id: BBa_B0050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaggatctcaagaagatcctttgatttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K404116_sequence/1
type: Sequence
persistent identity: BBa K404116 sequence
display id: BBa_K404116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtcctttactagaggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678019_sequence/1
type: Sequence
persistent identity: BBa K678019 sequence
display id: BBa_K678019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attccgatctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678012_sequence/1
type: Sequence
persistent identity: BBa K678012 sequence
display id: BBa_K678012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attccgataacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031941_sequence/1
type: Sequence
persistent identity: BBa K1031941 sequence
display id: BBa_K1031941_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatccttagctttcgctaaggatgatttttggatttcgcggccgctactagaaaggcctaccccttaggctttatgcaactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtgttgattttaaagaagatggaaacattcttggacacaagttggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagaccccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgaatgaactatacaaaataataatactagagccaggcatcaaataaaaacgaaaggctcagtcgaaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaccctggctcaccctcgggtggggcctttctgcgtttatatactaattagactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031224_sequence/1
type: Sequence
persistent identity: BBa K1031224 sequence
display id: BBa_K1031224_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcatttgctcaagcggccttgggcaattgatcaaatgcttaaaaagtctgcgcaagcgcggcttaatttcgctcgctccgatcattctaaaaattagaaacacattgaaaaacattaccttgaagtctgttttcagaccttggcacagccgttgcttgatgtcctgcgtactagagaaagaggagaaaactagaatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1015_sequence/1
type: Sequence
persistent identity: BBa Y1015 sequence
display id: BBa_Y1015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0025_sequence/1
type: Sequence
persistent identity: BBa B0025 sequence
display id: BBa_B0025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801012_sequence/1
type: Sequence
persistent identity: BBa K801012 sequence
display id: BBa_K801012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809006_sequence/1
type: Sequence
persistent identity: BBa K809006 sequence
display id: BBa_K809006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atattaattaagtttcgggtcccggctacgggacccggaacccccgagaggagttattatattta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0061_sequence/1
type: Sequence
persistent identity: BBa B0061 sequence
display id: BBa_B0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagtcaaaagcctccggtcggaggcttttgacttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486006_sequence/1
type: Sequence
persistent identity: BBa K1486006 sequence
display id: BBa_K1486006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccggagctcgggaccctctgccattctttccacctctgtacctgggcggccctgagattacaaccgagaactgcgagagagagcctatccacattcctgggtccatccagccacacggggctctgctcacagctgacggccactccggagaggtgctccaagtgtccctgaatgccgctaccttcctgggccaggagcctactgtgctgcgggggcagaccctggctgccctgctccccgagcagtggccagccctgcaggcagccctgcccccaggatgtccagatgccctccaatacagggccaccctcgactggccagctgctgggcacctcagcctgactgtgcatcgggtggctgaactcctgatcctggagttcgaacctaccgaggcctggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K629005_sequence/1
type: Sequence
persistent identity: BBa K629005 sequence
display id: BBa_K629005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcactgataataagcaatcattgcccgcgattaccctcgcggcgattggagttgtctacggcgatattggtaccagcccgttatatacacttcgtgaatgtttgtccggccagtttggttttggcgttgaacgcgatgccgtgtttggctttttatcgctgatcttctggctgctaatctttgtggtttccattaaatatctcaccttcgtgatgcgggcagataacgccggtgagggggggatcctgacgttgatgtcgcttgccgggcgtaatacgtcggcgcgaaccacatcaatgctggtgattatggggctaatcggcggcagctttttctatggtgaagtcgtcataacacccgctatttcggtgatgtcagccattgaaggtctggaaatcgtcgccccgcagctggatacctggatagttcccctctcaattatcgttctcactttattatttatgattcaaaagcatggcacggctatggtcggtaagctgtttgcgccgatcatgctgacctggtttttgattctggcaggactggggttacgtagcattattgctaacccggaagtgctgcatgcgctgaatccgatgtgggcggtgcatttcttccttgaatacaaaacggtttcttttattgcattaggggcagtggtgctgtcgattacgggggtcgaggcgctgtatgctgatatgggacactttggtaagttccctattcgtctggcgtggttcactgtcgtattaccttccttaactcttaattacttcggccagggagcgctgttgttaaagaacccggaagcgattaagaacccgttcttccttttggcaccggactgggcgctgatcccgctgctgatcatcgccgcactggcgacggtaatcgcctcgcaggcggttatctctggcgtcttttcattgacgcgtcaggcggtacgtctgggatatttgtcgccgatgcgcattattcacacctccgaaatggagtcagggcaaatctatattccgtttgtgaactggatgctctatgtcgcggtcgtgattgtgattgtcagctttgagcactccagcaacctggcggcggcgtacgggattgcggtgaccggaaccatggtgctgacgtctattctctcgactaccgtggcacgtcagaactggcactggaataagtattttgttgcgctgatcctgattgctttcctttgtgtcgatattccattgttcaccgctaacctcgataaactgctctccggcggctggttgccattgagcctcggtactgtgatgtttatcgtgatgaccacctggaagagcgagcgtttccgcttgctgcggcggatgcatgaacatggtaactctctggaagcgatgattgcttcgctggagaaatcaccgcctgttcgcgtgcccgggaccgcggtgtatatgtcgcgtgcaatcaacgtcattccctttgcgctgatgcataaccttaaacataacaaggtattgcatgagcgggtgattctgttaactctgcgcaccgaagacgctccatatgtccataacgtccgtcgggtacagattgaacaactgtcgcccactttctggcgcgtggtggcaagttatggttggcgagaaacgccaaacgtagaagaagttttccaccgctgcggtctggaaggattaagttgccggatgatggaaacctccttctttatgtcgcatgagtcgttgatcctcggcaaacgcccgtggtatttgcgtctgcgcggcaagctgtacttgctgctgcaacgtaatgcgctgcgtgcaccagatcaatttgaaatcccgccaaacagggttatcgaactgggtactcaggtcgaaatctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K404108_sequence/1
type: Sequence
persistent identity: BBa K404108 sequence
display id: BBa_K404108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031623_sequence/1
type: Sequence
persistent identity: BBa K1031623 sequence
display id: BBa_K1031623_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatggctagctcagtcctagggattatgctagctactagagaaagaggagaaatactagatgaagacaaaactgtttatcaataacgcctggatcgattctagtgaccagcagaccttcgagcgcatacaccccgtcagcagcgatgtggtgactgagagcgcaaacgccacagtgacggacgcgataaaggcggcgcaagcggccgaggaggcgttcaagacctggaaggccgttggaccttcagagcgtcgccgccttctcctaaaggtcgccgatgtcatggaaagtaaaacacccaagttcatcgaagtgatggccatggaggtgggagcttccgccctttgggccggattcaacgtccatgcgtctgccaatgtgttccgagaggctgcctcgctggctacccaaattcagggtgaaaccatcccaacggacaaagccgaaacgctctcaatgacactacgtcagccggtcggcccgatcctaagcatcgttccatggaacggcaccgcagtgcttgcggcacgagccatcgcttatccgctggtctgtggcaacactgtggtgttcaaaggctctgaatttagtcccgcgacgcatgccctgatcacccagtgcgtgcaggaagccgggctgcccgctggcgtgctcaattacctcaactcttcgcctgaccgttcgcccgagatcgctgacgcactgatctctgccaaggagatccgccgcatcaacttcacgggttccacccgcgtgggcagcattatcgcgcagaaagccgcgcaacacctcaagcgctgcctgctggagctcggcggcaagtccccgcttattgttctggatgatgcagacatcgatgcggcggtcaaggcagcggtgttcggtagcttcctgttccaaggtcagatctgcatgtccactgagcgcttgatcgttgatgagaagatagccgacgaatttgtcgcaaaatttgtcgaaaaaactaagcgcttgagcgcaggcgacccgtgcgtaactggcgactgcatcatcggcccgatggtctcgccaaattcgggtgagcggatcaatggtttgttcaaagacgcgatcgacaaaggggcaaaagttgtttgcggcggcttggcccaaggtgcgctcatgccggccacgatcctggatcacgtcaaatctgacatgcggatttacgatgaggagacctttggtcccatcaccgtggtaatccgttgtaaaggcgaagcagaggccgtccgcattgccaacgacagcgtctatggcctgtcgtcgggcgtatttggccgcgacatcaaccgcgctctacgcgtgggtatgtccatcgaatatggttctgtacacatcaacggttcgaccgcccagaacgaggcgcaggctccttacggaggcaccaagaacaccggctacgggcgcttcgacggccgtgctgtaatcgacgagttcacagagatcaagtggctgaccatcgaacctttcgagcagcaatatcccttctgaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031420_sequence/1
type: Sequence
persistent identity: BBa K1031420 sequence
display id: BBa_K1031420_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttatcgaaaaaacgtctcagctggtagaaattaactatttcacatattagcaaccaaccagcaacatccttatactagatcacacaggaaacctactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031113_sequence/1
type: Sequence
persistent identity: BBa K1031113 sequence
display id: BBa_K1031113_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcatttgctcaagcggccttgggcaattgatcaaatgcttaaaaagtctgcgcaagcgcggcttaatttcgctcgctccgatcattctaaaaattagaaacacattgaaaaacattaccttgaagtctgttttcagaccttggcacagccgttgcttgatgtcctgcactagatcacacaggaaacctactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K259006_sequence/1
type: Sequence
persistent identity: BBa K259006 sequence
display id: BBa_K259006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0440_sequence/1
type: Sequence
persistent identity: BBa C0440 sequence
display id: BBa_C0440_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaatattattactgagtaaaggattgttaccgcactaagcgggcaaaacctgaaaaaaattgcttgattcacgtcaggccgtttttttcaggtttttttttggagttttgccgcaaagcggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746916_sequence/1
type: Sequence
persistent identity: BBa I746916 sequence
display id: BBa_I746916_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0051_sequence/1
type: Sequence
persistent identity: BBa B0051 sequence
display id: BBa_B0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagtcaaaagcctccgaccggaggcttttgactt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0052_sequence/1
type: Sequence
persistent identity: BBa B0052 sequence
display id: BBa_B0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaaatcatccttagcgaaagctaaggattttttttatctg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678038_sequence/1
type: Sequence
persistent identity: BBa K678038 sequence
display id: BBa_K678038_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attccgatgcggttttcaaggataacagatggcaaaattgggatatcgtcccacgggctggtgaaaaccagcatcgtgaggattaatggtggacaattctctttatccctctacccggtgtaatctagagtgtccataccagttgtcaataagtttccaacaagccagtgccgttgtagttgagtatttgcagtatagtcaaccatagtcgtagccagtaatgtttacgtacaatatctacagaaaaatgaaataatttccacctcgtaaattagcacaccgtgacgaaatcccaagaacgtggcaacctagacaggcactaaaacccgtactacattacaattcaaccccgctccaacaacttcttctccctcttttctccggtgggggagggaaagagaggagggaggggggaaagttcgagatctggcaattcgaccctcggattggtttcccggttccgaatacggccggctttcccattccactttgctttccttgcttgctgcgtgctgcgtgctgctgtgttgcatgcagctagccttaatgagactacctatctttaatgtagagtagtatgagcagggaaagcctttatttcaactttctttcttccttgattttggttgataatatagatctgcaccatgatatagtattatagaatggatagatgaatgaatggatgaatggtatgtatacgtaagtacctcgaaagttagttaagtaaaaagtgaggggtaagttgagagccagccaaggctggggggctaaaatatgatgtgatcggatcacgagtatatgtcgtagttgttgtgtgtttttgtgtaggattggattggattggattgggttgagttagtggcattgccgacatagccattcaatgagtcgaggaatgaactggtttcccgcgccagtaaataacttactttagctgtggtggtagtaagtaagatataaccggttttaaagttatccggggtcatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1001_sequence/1
type: Sequence
persistent identity: BBa B1001 sequence
display id: BBa_B1001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcttcggcggggttttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486009_sequence/1
type: Sequence
persistent identity: BBa K1486009 sequence
display id: BBa_K1486009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcaaagaggagaaaatgtccggagctcgggaccctctgccattctttccacctctgtacctgggcggccctgagattacaaccgagaactgcgagagagagcctatccacattcctgggtccatccagccacacggggctctgctcacagctgacggccactccggagaggtgctccaagtgtccctgaatgccgctaccttcctgggccaggagcctactgtgctgcgggggcagaccctggctgccctgctccccgagcagtggccagccctgcaggcagccctgcccccaggatgtccagatgccctccaatacagggccaccctcgactggccagctgctgggcacctcagcctgactgtgcatcgggtggctgaactcctgatcctggagttcgaacctaccgaggcctggtaaggtggtggtggttctggtggtggtggttctatgaataaaatcctgttagttgatgatgaccgagagctgacttccctattaaaggagctgctcgagatggaaggcttcaacgtgattgttgcccacgatggggaacaggcgcttgatcttctggacgacagcattgatttacttttgcttgacgtaatgatgccgaagaaaaatggtatcgacacattaaaagcacttcgccagacacaccagacgcctgtcattatgttgacggcgcgcggcagtgaacttgatcgcgttctcggccttgagctgggcgcagatgactatctcccgaaaccgtttaatgatcgtgagctggtggcacgtattcgcgcgatcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031710_sequence/1
type: Sequence
persistent identity: BBa K1031710 sequence
display id: BBa_K1031710_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagtaaacttgatacttttatccaacatgctgtaaacgctgttccggtcagtggcacatctttgatctcctctctgtatggtgattcgctttcccatcgtggtggtgaaatctggttgggtagtctggctgctttgctggaagggctgggatttggtgagcgtttcgtgcgcaccgctttgtttcgtcttaataaagaaggctggctggatgtttcccgcatcgggcgacgcagtttctatagcctcagtgataaaggcttgcgcctgacgcgacgggcagaaagtaaaatttatcgcgcagagcaacctgcatgggatggtaaatggctcctgttgctctcggaaggtttagataaatcaacgctggctgatgtcaaaaagcagttgatctggcaaggttttggcgcactggcacccagcctgatggcatcgccgtcgcaaaaactggccgatgtacagacacttttgcatgaagcgggtgtggcggataacgtgatttgttttgaagcgcaaataccactggcgctttctcgcgcagcactgcgtgccagagtagaagagtgctggcatttaactgaacaaaatgccatgtacgaaacctttattcagtcattccgcccgctggtgccgcttttaaaagaggcggcagacgagttaaccccggagcgggcatttcatattcagcttttactgatccatttttatcgccgtgtcgtccttaaagacccattgttgccggaggagttgcttccggcacactgggcagggcatacggcgcgtcagctgtgtatcaacatttatcagcgcgtagcgcctgctgctttagcgttcgttagtgaaaaaggtgaaacctcggtcggtgaactgcctgcgccgggaagcctgtattttcaacgttttggcggcttgaatattgaacaggaggcgttatgccaatttatcagatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031311_sequence/1
type: Sequence
persistent identity: BBa K1031311 sequence
display id: BBa_K1031311_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagcactagaattaaagaggagaaatactagatgaaatcaaataaaaataatagcgacgatcgctccatcgttgccgaccttgcgctccccgaagtccatgctttagtttccaagctgcacttttctcccaacgaaggacgtatctggctagacgaaagccgatgcctgctcctacaagtggagacactgaaggatatatacaaggaattgcaggcctattctggcccagactacacgcgggagtttttgactcgtatcggcttcaccaccggccaacgcgatgcagaaatgattataaaaaaacaaggtatatcctctatcaaagaacagatttatgcaggtggtgtcttgcacgccttgcaagggtttctaacatctatacaagctggatcatcggcgctaaatgcggtcgatatgaagagcatggattatcatgctgaggcctactggcagaactctatcgaagctgaaattcacttagcgatgcatggtgtcagttcacatgcggtatgttggttttccgttgcgtattgctcagggtacctaagcgcatgcgctggaaaacccattgtcgtggaagagatcgaatgccaagcgatgggacacactcattgccgtattcaagcgaagcccgccgaaatgtgggcgctcagtcagtcggagcaatcccagatcaccacgcaccctattcccgatgatgatcagggcggcgagctcgtcattggttcgtcggcagtgttcaaggtgctccgtcacaaaacagcctgtgtggcagagactgatgcgactgttcttttacttggagaaagtgggagcggcaagagccttattgcacgcgagatccaccgtttgagcaacagagccgaccaagctttcgtcgaagtgaactgtgccgctatacccgatcaattaatcgagtcggagctgttcggcgtagagcgcggagcctttacaggtgcgaccgctacgcgagaggggcgtttcgaggcggctcatcaaggcactctcttccttgacgaaattgccaccctcagtatgaccgctcaaagtaaacttctccgtgtattgcagaatggcgaactagaacgtctcggtagcaaccggacaatccacaccagtgtccgactgattgccgccacgaacgctgatctgaaaaaagctgtccaagatggtcacttccgagaggatctttactatcgactgaacgtctttcccatccagattccccctctgcgagaacgccgcgatgatatttcactcattaccagcgtactcatcgctcgattttctaaacgccacggacgtaagttaaaaggcatatcgtctgccgccatgcaggtacttatctaccatgactggccaggcaatattcgagaattggagaacgtgattgagagagccataatcatggctcaagacgtcgattttttggacacccaccatttaacgacaattgaaggcaccctcactacacaagacttcctaagtcttaaccagaaaggtgatctaaccttgagcagcgagctaatacggaacgcggctgagaacgccaaccctaaagttctctcattagatgagtttgccgagcagatggtgcatcaagggtctattaacctggaccaggttcaagacgccatcacccgagcagctgtcaaacatagcggtggcaatatttcccgggcggcctcccttcttgggataactcgagcccggcttgattaccgcgtcaaaaagatcacatagactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031521_sequence/1
type: Sequence
persistent identity: BBa K1031521 sequence
display id: BBa_K1031521_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: ttcaaaatcattaatatagaaacagttaatcatcacgttaccgggactgcgctgtccctgcaattccgacttgatcaaaattgagcatgttttagtcactcactgatatttaatatattcttatctttaaatgtaaatagtttgttaattagatcacatttacatcacttacttttgccaaactacgcaatcggggaataaaagtaatcattaacatattaatgattactagatcacacaggaaagtactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K110012_sequence/1
type: Sequence
persistent identity: BBa K110012 sequence
display id: BBa_K110012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcaaaatttacggctttgaaaaagtaatttcgtgaccttcggtataaggttactactagattcaggtgctcatcagatgcaccacattctctataaaaaaaaatggtatctttcttatttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031510_sequence/1
type: Sequence
persistent identity: BBa K1031510 sequence
display id: BBa_K1031510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcacgactcactaaccattgcgttgctccaggcgcgagaagcggcgatgagttattttcgcccaatcgttaaacggcataatttgactgagcaacagtggcgcatcgtgcgtattctggcggaaagtccatcaatggattttcacgatctggcgtatcgcgcctgcattttgcgcccaagcctgaccgggatccttacgcggatggaacgcgacggtttagtgttgcgattaaagccgatcaatgatcagcggaagctctatatctcgctgaccaaagaggggcaggcgctgtataaccgcgctcagacacagattgaagaagcctatcggcagattgaagctcagtttactgccgagaaaatgcaacagttaacccatctgttagaggaatttattgctctgggtaattcccgacaagaagacatcccgggcgataatgaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031803_sequence/1
type: Sequence
persistent identity: BBa K1031803 sequence
display id: BBa_K1031803_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagactagatcacacaggaaacctactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031422_sequence/1
type: Sequence
persistent identity: BBa K1031422 sequence
display id: BBa_K1031422_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttatcgaaaaaacgtctcagctggtagaaattaactatttcacatattagcaaccaaccagcaacatccttatactagaattaaagaggagaaatactaatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0013_sequence/1
type: Sequence
persistent identity: BBa B0013 sequence
display id: BBa_B0013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031221_sequence/1
type: Sequence
persistent identity: BBa K1031221 sequence
display id: BBa_K1031221_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcatttgctcaagcggccttgggcaattgatcaaatgcttaaaaagtctgcgcaagcgcggcttaatttcgctcgctccgatcattctaaaaattagaaacacattgaaaaacattaccttgaagtctgttttcagaccttggcacagccgttgcttgatgtcctgcgtactagagtcacacaggaaaccactagaatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809005_sequence/1
type: Sequence
persistent identity: BBa K809005 sequence
display id: BBa_K809005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaactcctaacggggttcccgcgaagcgggaactaataataatataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486004_sequence/1
type: Sequence
persistent identity: BBa K1486004 sequence
display id: BBa_K1486004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggtggtggttctggtggtggtggttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0055_sequence/1
type: Sequence
persistent identity: BBa B0055 sequence
display id: BBa_B0055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2122000_sequence/1
type: Sequence
persistent identity: BBa K2122000 sequence
display id: BBa_K2122000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgtccaagccccccgacctcctgctgcggctgctccggggcgccccaaggcagcgggtctgcaccctgttcatcatcggcttcaagttcacgtttttcgtctccatcatgatctactggcacgttgtgggagagcccaaggagaaagggcagctctataacctgccagcagagatcccctgccccaccttgacaccccccaccccaccctcccacggccccactccaggcaacatcttcttcctggagacttcagaccggaccaaccccaacttcctgttcatgtgctcggtggagtcggccgccagaactcaccccgaatcccacgtgctggtcctgatgaaagggcttccgggtggcaacgcctctctgccccggcacctgggcatctcacttctgagctgcttcccgaatgtccagatgctcccgctggacctgcgggagctgttccgggacacacccctggccgactggtacgcggccgtgcaggggcgctgggagccctacctgctgcccgtgctctccgacgcctccaggatcgcactcatgtggaagttcggcggcatctacctggacacggacttcattgttctcaagaacctgcggaacctgaccaacgtgctgggcacccagtcccgctacgtcctcaacggcgcgttcctggccttcgagcgccggcacgagttcatggcgctgtgcatgcgggacttcgtggaccactacaacggctggatctggggtcaccagggcccgcagctgctcac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031100_sequence/1
type: Sequence
persistent identity: BBa K1031100 sequence
display id: BBa_K1031100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccgatcaagtacaagcctgaaatccagcactccgatttcaaggacctgaccaacctgatccacttccagagcacggaaggcaagatctggcttggcgaacaacgcatgctgttgctgcaagtttcagcaatggccagctttcgccgggaaatggtcaataccctgggcatcgaacgcgccaagggcttcttcctgcgccagggttaccagtccggcctgaaggatgccgaactggccaggaagcttagaccgaatgccagcgagtacgacatgttcctcgccggcccgcagctgcattcgctcaagggtctggtcaaggtccgccccaccgaggtcgatatcgacaaggaatgcgggcgcttctatgccgagatggagtggatcgactccttcgaggtggaaatctgccagaccgacctggggcagatgcaagacccggtgtgctggactctgctcggctacgcctgcgcctattcctcggcgttcatgggccgggaaatcatcttcaaggaagtaagctgccgcggctgcggcggcgacaagtgccgggtcattggcaagccggccgaagagtgggacgacgttgccagcttcaaacagtatttcaagaacgaccccatcatcgaggaactctacgagttgcaatcgcaactggtgtcgctgcgtaccaacctcgacaaacaggaaggccagtactacggcatcggtcagaccccggcctaccagaccgtgcgcaatatgatggacaaggccgcacagggcaaagtctcggtgctgctgcttggcgagaccggggtcggcaaggaggtcatcgcgcgtagcgtgcacctgcgcagcaaacgcgccgccgagccctttgtcgcggtgaactgtgcggcgatcccgccggacctgatcgagtccgaattgttcggcgtggaaaaaggcgccttcaccggcgccacccagtcacgcatgggccgcttcgagcgggccgacaagggcaccatcttccttgacgaggtgatcgaactcagcccgcgcgctcaggccagtctgctgcgcgtgctgcaagaaggcgagctggagcgagttggcgacaaccgcacgcgcaagatcgacgtaagggttatcgcagccacccacgaggacctggccgaagcggtcaaggccgggcgttttcgcgccgacctgtactaccggctgaacgttttcccggtggcgatcccggcgttgcgcgaacgccgcgaggacattccactgctggttgagcacttccttcagcgcttccaccaggagtacggcaagagaaccctcggcctttcagacaaagccctggaggcctgcctgcattacagttggccgggcaatatccgtgagctggagaacgtcatcgagcgcggcatcatcctcaccgatccgaacgaaagcatcagcgtgcaggcgctgttcccacgggcgccggaagagccgcagaccgccagcgagcgggtgtcgtcggacggcgtgctgattcagccaggcaatggccagggcagttggatcagccagttgttgagcagcggcctgagcctcgacgagatcgaggaaagcctgatgcgcgaagccatgcaacaggccaaccaaaacgtctccggtgccgcgcgcttgctcggcctaagccgaccggcactggcctatcggctgaagaaaatcggcatcgaaggctagactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2122150_sequence/1
type: Sequence
persistent identity: BBa K2122150 sequence
display id: BBa_K2122150_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccaagccccccgacctcctgctgcggctgctccggggcgccccaaggcagcgggtctgcaccctgttcatcatcggcttcaagttcacgtttttcgtctccatcatgatctactggcacgttgtgggagagcccaaggagaaagggcagctctataacctgccagcagagatcccctgccccaccttgacaccccccaccccaccctcccacggccccactccaggcaacatcttcttcctggagacttcagaccggaccaaccccaacttcctgttcatgtgctcggtggagtcggccgccagaactcaccccgaatcccacgtgctggtcctgatgaaagggcttccgggtggcaacgcctctctgccccggcacctgggcatctcacttctgagctgcttcccgaatgtccagatgctcccgctggacctgcgggagctgttccgggacacacccctggccgactggtacgcggccgtgcaggggcgctgggagccctacctgctgcccgtgctctccgacgcctccaggatcgcactcatgtggaagttcggcggcatctacctggacacggacttcattgttctcaagaacctgcggaacctgaccaacgtgctgggcacccagtcccgctacgtcctcaacggcgcgttcctggccttcgagcgccggcacgagttcatggcgctgtgcatgcgggacttcgtggaccactacaacggctggatctggggtcaccagggcccgcagctgctcacgcgggtcttcaagaagtggtgttccatccgcagcctggccgagagccgcgcctgccgcggcgtcaccaccctgccccctgaggccttctaccccatcccctggcaggactggaagaagtactttgaggacatcaaccccgaggagctgccgcggctgctcagtgccacctatgctgtccacgtgtggaacaagaagagccagggcacgcggttcgaggccacgtccagggcactgctggcccagctgcatgcccgctactgccccacgacgcacgaggccatgaaaatgtacttgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678037_sequence/1
type: Sequence
persistent identity: BBa K678037 sequence
display id: BBa_K678037_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attccgatggctccgaggctactggagtggctgcaactgctcattggaaaaggatatgaggtgcggggggtcaaaccgattgggcccggtagctcattacttgactgacggtctcatctatctggccctgcgcaaacctcactagttcttcgctaagtcggagagacagcgactagattcggacgaagtgactttccccccggaaccgccaacctactggacaacaaactactctagtgacttccaggtttaggttcaaatcatcagtgcggcatttcggtggtctagaactgggagcaacggtgcaattggccctagtaacaggcaagtaattctcatccccgctagcattcgctccgtgtgcggacacaatagctattgcgcactgtatgtagactgtcacgcttgtcaaccttcgccgctgcatcttactaaagagctaccaaccaacacatgcagtagctcgttgttgatacgccgtacattcttggctcttgcgtatatatacatttgtgattcagatttaccctgcctcgcacattgcttctttgctcatcttcccttccctaagtcgacatggtgaaataatgtttgaggtggcgcctgcaattacatactcatcatattaaatgggcttgccgcgggaaagacgtaaacgccactatgggtttgctaacaaattgattgacagagttgccgttgaaggcctggtctaatgcaggcccgcactatgagacgcggcagacgcagccactctagtcttcaattccaacaattgaggatgtggccatgccaagtcactcatgcataaactatgctggccagtccgggctcacggagcttcgagcaccaatcgccgtccagacagatagcatgtcttcagatccagtccctgcttcgttatctgcatgcgtgtgcattgacaatgctgttctttgccctcagaatatttcccaatacacattgtttgcctctcactttcgagtatattggcatcagacgtcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031410_sequence/1
type: Sequence
persistent identity: BBa K1031410 sequence
display id: BBa_K1031410_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggaactacggcatttacgctatttcgtcgcagtggcgcaggcactgaactttacccgtgcggcggaaaaactgcatacctcacagccttcgttaagcagccagatccgcgatcttgaaaactgtgtcggtgttccgttactggtgagggataagcgcaaagtcgcgttgacggcggcgggagaatgttttctccaggacgcgctggcaatcctcgaacaagcggaaaatgccaaattacgggcgcggaaaattgttcaggaagacagacaattaaccattggctttgtgccatcggcggaagtgaatttactgccaaaagtattaccgatgtttcgtctcagacagccagacaccttgattgagctggtgagtttaatcaccacgcaacaggaggaaaaaattcgccgtggtgaactcgacgtcggcttaatgcgccatcctgtttatagcccggagattgattatctggagctttttgacgaaccattagtggttgtgttaccggttgatcaccctttagcacatgaaaaagagatcaccgccgcgcaactggatggcgtgaatttcgtcagtaccgatccggtgtattccggttcgcttgcgccgatcgttaaagcgtggtttgcgcaagaaaacagccagccaaatatcgtccaggtggcaacgaatattctggtgaccatgaatctggtgggcatggggctgggcgtcactttgatacccggttatatgaataattttaataccggacaggttgtttttcgtcccattgccggaaatgtgccttccattgctttactgatggcgtggaagaaaggggagatgaagccggcattacgcgatttcatcgccattgtgcaggaacgtttggcaagcgtaacggcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678039_sequence/1
type: Sequence
persistent identity: BBa K678039 sequence
display id: BBa_K678039_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attccgataagtcttccgttacccttgcaattgatcgaaaggagaagaagtcaagaaaagaaagaaatcgagacaaaacgcccaaaacaaaagcgagtttctctactttcacaatctacctactctaccctaccacctctctgatctcatcactctatcttacttctctccttttcgaattttttataatcaagcagtgggcgttcatcatcactttttgctgccaattggtgttgtatagggttgcaggcgtatgggaaggttacggggtcttgaggcgaaccgcggggtctttctttgtttttgatgctgttacaaacttctcaccgattttgatctcattttatctacctacctacctcttgtttgattgattgttgactctatgccgttgactaccggctgggtcacgtgtgcgtgtacaatacaacctacttatgtccgttgaatcgaacatgagaggtgtactatctattccatttcatatatggtcgaatcggatcgatcatcctgtgtatgccaagtccttcatggatgcacttggatgtctgatgttattgatcaagaatcaaaagaatcaaagtcacctgaaaccgggatcttattgtatcactatttattcccataaatagaacaactcacacgcgcactcccttttatcaatgtcaccatgacgccgtactcccctcataaatagcaataacctcacagcccagacagatagagcagatagacacatggatagaacagcccaacatggtatcatgaactgaacttcagccgtaaacaccactacatagatatagaaagcatgaaaaggaaagggaaaaggggaaagagaagaaaaggaggctgggaatgtagaagaggtaggtaggtaggaattaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0021_sequence/1
type: Sequence
persistent identity: BBa B0021 sequence
display id: BBa_B0021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaataataaaaaagccggattaataatctggctttttatattctct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486007_sequence/1
type: Sequence
persistent identity: BBa K1486007 sequence
display id: BBa_K1486007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccggagctcgggacagcattggccctcacgccctgaggaacgccatgtttgctctggaaagcgctccaaacctgcgggctctggccgaagtcgcaacacaaactgtgagagaactgactggcttcgatcgggtgatgctgtacaaatttgcccctgacgccactggagagatgattgctgaggccagacgggagggcatgcaggcttttctgggccacaggtttcccgcatcccacacccctgcacaagctagggccctctacacaagacacctgctccggctgaccgcagacaccagggctgcagcagtgcccctcgaccccgtgctgaatccccagacaaatgctcctacacctctgggcggagctgtcctcagagctacatccccaatgcacatgcagtacctgaggaatatgggagtgggctcctccctgagcgtcagcgtcgtggtcggcggccagctgtggggactgattgtctgccaccatcagacaccctacgtgctgccaccagatctgcggaccaccctggaggagctggggagaaagctgtccgggcaggtgcagaggaaagaagcctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031622_sequence/1
type: Sequence
persistent identity: BBa K1031622 sequence
display id: BBa_K1031622_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgaagacaaaactgtttatcaataacgcctggatcgattctagtgaccagcagaccttcgagcgcatacaccccgtcagcagcgatgtggtgactgagagcgcaaacgccacagtgacggacgcgataaaggcggcgcaagcggccgaggaggcgttcaagacctggaaggccgttggaccttcagagcgtcgccgccttctcctaaaggtcgccgatgtcatggaaagtaaaacacccaagttcatcgaagtgatggccatggaggtgggagcttccgccctttgggccggattcaacgtccatgcgtctgccaatgtgttccgagaggctgcctcgctggctacccaaattcagggtgaaaccatcccaacggacaaagccgaaacgctctcaatgacactacgtcagccggtcggcccgatcctaagcatcgttccatggaacggcaccgcagtgcttgcggcacgagccatcgcttatccgctggtctgtggcaacactgtggtgttcaaaggctctgaatttagtcccgcgacgcatgccctgatcacccagtgcgtgcaggaagccgggctgcccgctggcgtgctcaattacctcaactcttcgcctgaccgttcgcccgagatcgctgacgcactgatctctgccaaggagatccgccgcatcaacttcacgggttccacccgcgtgggcagcattatcgcgcagaaagccgcgcaacacctcaagcgctgcctgctggagctcggcggcaagtccccgcttattgttctggatgatgcagacatcgatgcggcggtcaaggcagcggtgttcggtagcttcctgttccaaggtcagatctgcatgtccactgagcgcttgatcgttgatgagaagatagccgacgaatttgtcgcaaaatttgtcgaaaaaactaagcgcttgagcgcaggcgacccgtgcgtaactggcgactgcatcatcggcccgatggtctcgccaaattcgggtgagcggatcaatggtttgttcaaagacgcgatcgacaaaggggcaaaagttgtttgcggcggcttggcccaaggtgcgctcatgccggccacgatcctggatcacgtcaaatctgacatgcggatttacgatgaggagacctttggtcccatcaccgtggtaatccgttgtaaaggcgaagcagaggccgtccgcattgccaacgacagcgtctatggcctgtcgtcgggcgtatttggccgcgacatcaaccgcgctctacgcgtgggtatgtccatcgaatatggttctgtacacatcaacggttcgaccgcccagaacgaggcgcaggctccttacggaggcaccaagaacaccggctacgggcgcttcgacggccgtgctgtaatcgacgagttcacagagatcaagtggctgaccatcgaacctttcgagcagcaatatcccttctgaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11013_sequence/1
type: Sequence
persistent identity: BBa I11013 sequence
display id: BBa_I11013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtttatgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801011_sequence/1
type: Sequence
persistent identity: BBa K801011 sequence
display id: BBa_K801011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagattgataagacttttctagttgcatatcttttatatttaaatcttatctattagttaattttttgtaatttatccttatatatagtctggttattctaaaatatcatttcagtatctaaaaattcccctcttttttcagttatatcttaacaggcgacagtccaaatgttgatttatcccagtccgattcatcagggttgtgaagcattttgtcaatggtcgaaatcacatcagtaatagtgcctcttacttgcctcatagaatttctttctcttaacgtcaccgtttgtcttttatagtttcgaaatctatggtgataccaaatggtgttcccaattcatcgttacgggcgtattttttaccaattgaagtattggaatcgtcaattttaaagtatatctctcttttacgtaaagcctgcgagatcctcttaagtatagcggggaagccatcgttattcgatattgtcgtaacaaatactttgagcggcgctatctgtaatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0022_sequence/1
type: Sequence
persistent identity: BBa B0022 sequence
display id: BBa_B0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0017_sequence/1
type: Sequence
persistent identity: BBa B0017 sequence
display id: BBa_B0017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486003_sequence/1
type: Sequence
persistent identity: BBa K1486003 sequence
display id: BBa_K1486003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggtggttctggtggtggttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1840004_sequence/1
type: Sequence
persistent identity: BBa K1840004 sequence
display id: BBa_K1840004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtttctaaaggtgaagaagacaacatggctatcatcaaagaatttatgcgtttcaaagttcacatggaaggttctgtgaacggtcacgaatttgaaatcgaaggtgaaggtgaaggtcgtccgtatgaaggcacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtctccgcagttcatgtacggttctaaagcgtatgttaaacacccggctgacatcccggactacctgaaactgtctttcccggaaggtttcaaatgggaacgtgttatgaactttgaagacggtggtgttgttaccgttacccaggactcttctctgcaagacggtgaatttatctacaaagttaaactgcgtggcaccaacttcccgtctgacggtccggttatgcagaaaaaaacgatgggttgggaagcgtcttctgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaacagcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacaaagctaaaaagccggttcaactgccgggtgcttacaacgtgaacatcaaactggacatcacctctcacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactctaccggcggtatggacgaactgtataaatgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K780000_sequence/1
type: Sequence
persistent identity: BBa K780000 sequence
display id: BBa_K780000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagaagaagtgtgaaaaagcgcagctgaaatagctgcgcttttttgtgtcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031301_sequence/1
type: Sequence
persistent identity: BBa K1031301 sequence
display id: BBa_K1031301_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttaataaatttatgaaatcgtggttgtgagttttcataatatggtgaagcttgcccgccatggcaagtgcatttcggcagctgttggcgaccgcggaaggggtttacagccgtctggagctaggctttctggcgctcattaaaataaaaatccttataaaacagtatcctagcttttatgtctgaggctgcttagtcaacctggcacggtactggctacgagtcccgctactagcacacaggaaacctactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataatactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031931_sequence/1
type: Sequence
persistent identity: BBa K1031931 sequence
display id: BBa_K1031931_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagcaaagaggagaaatactagatgcgggaaacaaaagagcagcctatcctagctggtacgggaaggtgtttagttctaattgggtagaggcgcggggaggtgttgccaatgttgtcgatccgtccaatggagacattcttggcattacgggtgttgctaacggcgaagatgtcgatgctgctgtgaacgcagctaagagagcgcaaaaggaatgggccgcaataccatttagtgaaagagccgccattgtccgcaaggctgccgaaaaactaaaggagcgcgagtatgagttcgccgattggaacgtacgggaatgcggcgcaattcgtccgaagggcttatgggaggccggaattgcgtatgagcaaatgcatcaagctgcgggtctagcttctttgcctaacggtacattgtttccatcggcagttccagggcgcatgaatctttgtcagcgcgttccagttggcgtggtcggcgtaattgcaccttggaatttcccgttgtttctagcaatgcgttcggtagcaccagccttagcgttgggtaatgcggtgatcttaaagcccgaccttcagactgctgtcaccgggggggcgctcattgccgaaatcttttccgacgctggcatgccggacggtgttcttcacgttcttcctggtggagcggacgtaggagagtcaatggttgcgaactccggaattaacatgatttcttttaccgggtccacacaggtgggccggttgatcggagagaaatgcgggagaatgctgaaaaaggttgcgcttgaactgggtggtaataatgtccacatcgtgttgcctgacgccgatttagaaggggctgtcagctgcgctgcttggggtacgtttttgcatcagggccaagtgtgcatggccgccggacgtcatttagtacatagggacgttgctcagcaatatgcagagaaactggcgctacgtgccaagaacttagtggtgggggatccaaactcggatcaagtgcatctcggcccgcttatcaatgagaaacaggtagttcgcgtccacgcgctcgttgaatctgcgcaaagggccggtgctcaggttttggcgggaggtacgtatcaagatcgctactaccaagctaccgtaatcatggatgtgaagccggagatggaggttttcaaatctgaaattttcggcccggtggctccgatcactgtatttgacagtattgaagaggcgattgaattggcaaactgttcggagtatgggttggccgcatctatccatactagggcgttggcgactggcctagacatcgcaaagcgtctaaataccggtatggtccatattaatgaccagccaattaactgtgagccgcatgttcccttcggaggaatgggtgcctcgggtagcggaggccggtttggcggacctgcaagtattgaagaatttactcaatctcaatggattagtatggttgagaagccagctaattacccattttgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K653001_sequence/1
type: Sequence
persistent identity: BBa K653001 sequence
display id: BBa_K653001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K392003_sequence/1
type: Sequence
persistent identity: BBa K392003 sequence
display id: BBa_K392003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1008_sequence/1
type: Sequence
persistent identity: BBa B1008 sequence
display id: BBa_B1008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgccaaaaaccccgcccctgacagggcggggtttttccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031625_sequence/1
type: Sequence
persistent identity: BBa K1031625 sequence
display id: BBa_K1031625_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggattgtgctagctactagagaaagaggagaaatactagatgaagacaaaactgtttatcaataacgcctggatcgattctagtgaccagcagaccttcgagcgcatacaccccgtcagcagcgatgtggtgactgagagcgcaaacgccacagtgacggacgcgataaaggcggcgcaagcggccgaggaggcgttcaagacctggaaggccgttggaccttcagagcgtcgccgccttctcctaaaggtcgccgatgtcatggaaagtaaaacacccaagttcatcgaagtgatggccatggaggtgggagcttccgccctttgggccggattcaacgtccatgcgtctgccaatgtgttccgagaggctgcctcgctggctacccaaattcagggtgaaaccatcccaacggacaaagccgaaacgctctcaatgacactacgtcagccggtcggcccgatcctaagcatcgttccatggaacggcaccgcagtgcttgcggcacgagccatcgcttatccgctggtctgtggcaacactgtggtgttcaaaggctctgaatttagtcccgcgacgcatgccctgatcacccagtgcgtgcaggaagccgggctgcccgctggcgtgctcaattacctcaactcttcgcctgaccgttcgcccgagatcgctgacgcactgatctctgccaaggagatccgccgcatcaacttcacgggttccacccgcgtgggcagcattatcgcgcagaaagccgcgcaacacctcaagcgctgcctgctggagctcggcggcaagtccccgcttattgttctggatgatgcagacatcgatgcggcggtcaaggcagcggtgttcggtagcttcctgttccaaggtcagatctgcatgtccactgagcgcttgatcgttgatgagaagatagccgacgaatttgtcgcaaaatttgtcgaaaaaactaagcgcttgagcgcaggcgacccgtgcgtaactggcgactgcatcatcggcccgatggtctcgccaaattcgggtgagcggatcaatggtttgttcaaagacgcgatcgacaaaggggcaaaagttgtttgcggcggcttggcccaaggtgcgctcatgccggccacgatcctggatcacgtcaaatctgacatgcggatttacgatgaggagacctttggtcccatcaccgtggtaatccgttgtaaaggcgaagcagaggccgtccgcattgccaacgacagcgtctatggcctgtcgtcgggcgtatttggccgcgacatcaaccgcgctctacgcgtgggtatgtccatcgaatatggttctgtacacatcaacggttcgaccgcccagaacgaggcgcaggctccttacggaggcaccaagaacaccggctacgggcgcttcgacggccgtgctgtaatcgacgagttcacagagatcaagtggctgaccatcgaacctttcgagcagcaatatcccttctgaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031111_sequence/1
type: Sequence
persistent identity: BBa K1031111 sequence
display id: BBa_K1031111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctagggactgtgctagctactagagaaagaggagaaatactagatgccgatcaagtacaagcctgaaatccagcactccgatttcaaggacctgaccaacctgatccacttccagagcacggaaggcaagatctggcttggcgaacaacgcatgctgttgctgcaagtttcagcaatggccagctttcgccgggaaatggtcaataccctgggcatcgaacgcgccaagggcttcttcctgcgccagggttaccagtccggcctgaaggatgccgaactggccaggaagcttagaccgaatgccagcgagtacgacatgttcctcgccggcccgcagctgcattcgctcaagggtctggtcaaggtccgccccaccgaggtcgatatcgacaaggaatgcgggcgcttctatgccgagatggagtggatcgactccttcgaggtggaaatctgccagaccgacctggggcagatgcaagacccggtgtgctggactctgctcggctacgcctgcgcctattcctcggcgttcatgggccgggaaatcatcttcaaggaagtaagctgccgcggctgcggcggcgacaagtgccgggtcattggcaagccggccgaagagtgggacgacgttgccagcttcaaacagtatttcaagaacgaccccatcatcgaggaactctacgagttgcaatcgcaactggtgtcgctgcgtaccaacctcgacaaacaggaaggccagtactacggcatcggtcagaccccggcctaccagaccgtgcgcaatatgatggacaaggccgcacagggcaaagtctcggtgctgctgcttggcgagaccggggtcggcaaggaggtcatcgcgcgtagcgtgcacctgcgcagcaaacgcgccgccgagccctttgtcgcggtgaactgtgcggcgatcccgccggacctgatcgagtccgaattgttcggcgtggaaaaaggcgccttcaccggcgccacccagtcacgcatgggccgcttcgagcgggccgacaagggcaccatcttccttgacgaggtgatcgaactcagcccgcgcgctcaggccagtctgctgcgcgtgctgcaagaaggcgagctggagcgagttggcgacaaccgcacgcgcaagatcgacgtaagggttatcgcagccacccacgaggacctggccgaagcggtcaaggccgggcgttttcgcgccgacctgtactaccggctgaacgttttcccggtggcgatcccggcgttgcgcgaacgccgcgaggacattccactgctggttgagcacttccttcagcgcttccaccaggagtacggcaagagaaccctcggcctttcagacaaagccctggaggcctgcctgcattacagttggccgggcaatatccgtgagctggagaacgtcatcgagcgcggcatcatcctcaccgatccgaacgaaagcatcagcgtgcaggcgctgttcccacgggcgccggaagagccgcagaccgccagcgagcgggtgtcgtcggacggcgtgctgattcagccaggcaatggccagggcagttggatcagccagttgttgagcagcggcctgagcctcgacgagatcgaggaaagcctgatgcgcgaagccatgcaacaggccaaccaaaacgtctccggtgccgcgcgcttgctcggcctaagccgaccggcactggcctatcggctgaagaaaatcggcatcgaaggctagactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031421_sequence/1
type: Sequence
persistent identity: BBa K1031421 sequence
display id: BBa_K1031421_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttatcgaaaaaacgtctcagctggtagaaattaactatttcacatattagcaaccaaccagcaacatccttatactagatcacacaggaaagtactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031520_sequence/1
type: Sequence
persistent identity: BBa K1031520 sequence
display id: BBa_K1031520_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcaaaatcattaatatagaaacagttaatcatcacgttaccgggactgcgctgtccctgcaattccgacttgatcaaaattgagcatgttttagtcactcactgatatttaatatattcttatctttaaatgtaaatagtttgttaattagatcacatttacatcacttacttttgccaaactacgcaatcggggaataaaagtaatcattaacatattaatgattactagatcacacaggaaacctactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18913_sequence/1
type: Sequence
persistent identity: BBa J18913 sequence
display id: BBa_J18913_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1010_sequence/1
type: Sequence
persistent identity: BBa B1010 sequence
display id: BBa_B1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgccgcaaaccccgcccctgacagggcggggtttcgccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K933000_sequence/1
type: Sequence
persistent identity: BBa K933000 sequence
display id: BBa_K933000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctaggggatccgttgacggctagctcagtcctaggtacagtgctagcttctagaccgaagccgccctcactcgttaagcaagatggcaacttatgaagatcttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactcaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctcaaactgtccttcccggaaggtttcaaatgggaacgtgttcttaacttcgaagacggtggtgttgttaccgttacccaggactcttccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctcaaaggtgaaatcaaaatacgtctcaaacttaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaacgtaaaggcgaagaactgtttaccggtgtggttccgattctggtggaactggatggtgatgttaatggtcataaattcagcgttcgtggtgaaggcgaaggtgatgccacgaatggtaaactgaccctgaaatttatctgcaccacaggtaaactgccggttccgtggccgaccctggttaccaccctgacctatggtgttcagtgtttcgcacgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggttatgttcaggaacgtaccattagctttaaagatgacggcacctataaaacccgtgccgaagttaaattcgaaggcgataccctggtgaatcgtatcgaactgaaaggcatcgattttaaagaggatggtaatatcctgggccataaactggaatataattttaacagccataacgtgtatatcaccgcagataaacagaaaaacggcattaaagcgaactttaaaatccgccataatgtggaagatggtagcgttcagctggcagatcattatcagcagaatacgccgatcggtgatggtccggttctgctgccggataatcattatctgagcacccagagcgttctgagtaaagatccgaatgaaaaacgtgatcacatggtgctgttagagttcgttaccgcagcaggtattacacatggtatggatgaactgtataaatgataaggccggccgtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacaattgactagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486001_sequence/1
type: Sequence
persistent identity: BBa K1486001 sequence
display id: BBa_K1486001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcaaagaggagaaaatgaataaaatcctgttagttgatgatgaccgagagctgacttccctattaaaggagctgctcgagatggaaggcttcaacgtgattgttgcccacgatggggaacaggcgcttgatcttctggacgacagcattgatttacttttgcttgacgtaatgatgccgaagaaaaatggtatcgacacattaaaagcacttcgccagacacaccagacgcctgtcattatgttgacggcgcgcggcagtgaacttgatcgcgttctcggccttgagctgggcgcagatgactatctcccgaaaccgtttaatgatcgtgagctggtggcacgtattcgcgcgatcctgcgccgttcgcactggagcgagcaacagcaaaacaacgacaacggttcaccgacactggaagttgatgccttagtgctgaatccaggccgtcaggaagccagcttcgacgggcaaacgctggagttaaccggtactgagtttaccctgctctatttgctggcacagcatctgggtcaggtggtttcccgtgaacatttaagccaggaagtgttgggcaaacgcctgacgcctttcgaccgcgctattgatatgcacatttccaacctgcgtcgtaaactgccggatcgtaaagatggtcacccgtggtttaaaaccttgcgtggtcgcggctatctgatggtttctgcttcataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031804_sequence/1
type: Sequence
persistent identity: BBa K1031804 sequence
display id: BBa_K1031804_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagactagatcacacaggaaagtactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0024_sequence/1
type: Sequence
persistent identity: BBa B0024 sequence
display id: BBa_B0024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486000_sequence/1
type: Sequence
persistent identity: BBa K1486000 sequence
display id: BBa_K1486000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaataaaatcctgttagttgatgatgaccgagagctgacttccctattaaaggagctgctcgagatggaaggcttcaacgtgattgttgcccacgatggggaacaggcgcttgatcttctggacgacagcattgatttacttttgcttgacgtaatgatgccgaagaaaaatggtatcgacacattaaaagcacttcgccagacacaccagacgcctgtcattatgttgacggcgcgcggcagtgaacttgatcgcgttctcggccttgagctgggcgcagatgactatctcccgaaaccgtttaatgatcgtgagctggtggcacgtattcgcgcgatcctgcgccgttcgcactggagcgagcaacagcaaaacaacgacaacggttcaccgacactggaagttgatgccttagtgctgaatccaggccgtcaggaagccagcttcgacgggcaaacgctggagttaaccggtactgagtttaccctgctctatttgctggcacagcatctgggtcaggtggtttcccgtgaacatttaagccaggaagtgttgggcaaacgcctgacgcctttcgaccgcgctattgatatgcacatttccaacctgcgtcgtaaactgccggatcgtaaagatggtcacccgtggtttaaaaccttgcgtggtcgcggctatctgatggtttctgcttcataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031621_sequence/1
type: Sequence
persistent identity: BBa K1031621 sequence
display id: BBa_K1031621_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgaagacaaaactgtttatcaataacgcctggatcgattctagtgaccagcagaccttcgagcgcatacaccccgtcagcagcgatgtggtgactgagagcgcaaacgccacagtgacggacgcgataaaggcggcgcaagcggccgaggaggcgttcaagacctggaaggccgttggaccttcagagcgtcgccgccttctcctaaaggtcgccgatgtcatggaaagtaaaacacccaagttcatcgaagtgatggccatggaggtgggagcttccgccctttgggccggattcaacgtccatgcgtctgccaatgtgttccgagaggctgcctcgctggctacccaaattcagggtgaaaccatcccaacggacaaagccgaaacgctctcaatgacactacgtcagccggtcggcccgatcctaagcatcgttccatggaacggcaccgcagtgcttgcggcacgagccatcgcttatccgctggtctgtggcaacactgtggtgttcaaaggctctgaatttagtcccgcgacgcatgccctgatcacccagtgcgtgcaggaagccgggctgcccgctggcgtgctcaattacctcaactcttcgcctgaccgttcgcccgagatcgctgacgcactgatctctgccaaggagatccgccgcatcaacttcacgggttccacccgcgtgggcagcattatcgcgcagaaagccgcgcaacacctcaagcgctgcctgctggagctcggcggcaagtccccgcttattgttctggatgatgcagacatcgatgcggcggtcaaggcagcggtgttcggtagcttcctgttccaaggtcagatctgcatgtccactgagcgcttgatcgttgatgagaagatagccgacgaatttgtcgcaaaatttgtcgaaaaaactaagcgcttgagcgcaggcgacccgtgcgtaactggcgactgcatcatcggcccgatggtctcgccaaattcgggtgagcggatcaatggtttgttcaaagacgcgatcgacaaaggggcaaaagttgtttgcggcggcttggcccaaggtgcgctcatgccggccacgatcctggatcacgtcaaatctgacatgcggatttacgatgaggagacctttggtcccatcaccgtggtaatccgttgtaaaggcgaagcagaggccgtccgcattgccaacgacagcgtctatggcctgtcgtcgggcgtatttggccgcgacatcaaccgcgctctacgcgtgggtatgtccatcgaatatggttctgtacacatcaacggttcgaccgcccagaacgaggcgcaggctccttacggaggcaccaagaacaccggctacgggcgcttcgacggccgtgctgtaatcgacgagttcacagagatcaagtggctgaccatcgaacctttcgagcagcaatatcccttctgaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0063_sequence/1
type: Sequence
persistent identity: BBa B0063 sequence
display id: BBa_B0063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacgcgaagtaatcttttcggttttaaagaaaaagggcagggtggtgacaccttgcccgtttttttgccgga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031917_sequence/1
type: Sequence
persistent identity: BBa K1031917 sequence
display id: BBa_K1031917_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggattgtgctagcactagaattaaagaggagaaatactagatggatttttgcttattgaacgagaaaagtcagatcttcgtccacgccgagccctatgcagtctccgattatgttaaccagtatgtcggtacgcactctattcgcctgcccaagggcgggcgcccggcaggcaggctgcaccacagaatcttcggatgcctcgacctgtgtcgaatcagctacggcggtagcgtgagggtaatctcgcctggattagagacctgttatcatctgcaaataatactcaaaggccattgcctgtggcgtggccatggccaggagcactattttgcgccgggcgaactattgctgctcaatccggatgaccaagccgacctgacctattcagaagattgcgagaaatttatcgttaaattgccctcagtggtccttgatcgggcatgcagtgacaacaattggcacaagccgagggagggtatccgtttcgccgcgcgacacaatctccagcaactcgatggctttatcaatctactcgggttagtttgtgacgaagcggaacatacaaagtcgatgcctcgggtccaagagcactatgcggggatcatcgcttccaagctgctcgaaatgctgggcagcaatgtcagccgtgaaattttcagcaaaggtaacccgtctttcgagcgagtcgttcaattcattgaggagaatctcaaacggaatatcagccttgagcggttagcggagctggcgatgatgagtccacgctcgctctacaatttgttcgagaagcatgccggcaccacgccgaagaactacatccgcaaccgcaagctcgaaagcatccgcgcctgcttgaacgatcccagtgccaatgtgcgtagtataactgagatagccctagactacggcttcttacatttgggacgcttcgctgaaaactataggagcgcgttcggcgagttgccttccgacaccctgcgtcaatgcaaaaaggaagtggcttgaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031620_sequence/1
type: Sequence
persistent identity: BBa K1031620 sequence
display id: BBa_K1031620_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagacaaaactgtttatcaataacgcctggatcgattctagtgaccagcagaccttcgagcgcatacaccccgtcagcagcgatgtggtgactgagagcgcaaacgccacagtgacggacgcgataaaggcggcgcaagcggccgaggaggcgttcaagacctggaaggccgttggaccttcagagcgtcgccgccttctcctaaaggtcgccgatgtcatggaaagtaaaacacccaagttcatcgaagtgatggccatggaggtgggagcttccgccctttgggccggattcaacgtccatgcgtctgccaatgtgttccgagaggctgcctcgctggctacccaaattcagggtgaaaccatcccaacggacaaagccgaaacgctctcaatgacactacgtcagccggtcggcccgatcctaagcatcgttccatggaacggcaccgcagtgcttgcggcacgagccatcgcttatccgctggtctgtggcaacactgtggtgttcaaaggctctgaatttagtcccgcgacgcatgccctgatcacccagtgcgtgcaggaagccgggctgcccgctggcgtgctcaattacctcaactcttcgcctgaccgttcgcccgagatcgctgacgcactgatctctgccaaggagatccgccgcatcaacttcacgggttccacccgcgtgggcagcattatcgcgcagaaagccgcgcaacacctcaagcgctgcctgctggagctcggcggcaagtccccgcttattgttctggatgatgcagacatcgatgcggcggtcaaggcagcggtgttcggtagcttcctgttccaaggtcagatctgcatgtccactgagcgcttgatcgttgatgagaagatagccgacgaatttgtcgcaaaatttgtcgaaaaaactaagcgcttgagcgcaggcgacccgtgcgtaactggcgactgcatcatcggcccgatggtctcgccaaattcgggtgagcggatcaatggtttgttcaaagacgcgatcgacaaaggggcaaaagttgtttgcggcggcttggcccaaggtgcgctcatgccggccacgatcctggatcacgtcaaatctgacatgcggatttacgatgaggagacctttggtcccatcaccgtggtaatccgttgtaaaggcgaagcagaggccgtccgcattgccaacgacagcgtctatggcctgtcgtcgggcgtatttggccgcgacatcaaccgcgctctacgcgtgggtatgtccatcgaatatggttctgtacacatcaacggttcgaccgcccagaacgaggcgcaggctccttacggaggcaccaagaacaccggctacgggcgcttcgacggccgtgctgtaatcgacgagttcacagagatcaagtggctgaccatcgaacctttcgagcagcaatatcccttctgaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K864600_sequence/1
type: Sequence
persistent identity: BBa K864600 sequence
display id: BBa_K864600_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgttcagaacgctcggtcttgcacaccgggcgttttttctttgtgagtcca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486052_sequence/1
type: Sequence
persistent identity: BBa K1486052 sequence
display id: BBa_K1486052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatgataaacgaaagcgtcatcccgggtgtagtagta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K187025_sequence/1
type: Sequence
persistent identity: BBa K187025 sequence
display id: BBa_K187025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgaggaggtctagaaaaaaaaaccccgcccctgacagggcggggttttttttctgcagtg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031922_sequence/1
type: Sequence
persistent identity: BBa K1031922 sequence
display id: BBa_K1031922_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagcactagaattaaagaggagaaatactagatggaaatggaaatcaaagcagcaatagttcgccaaaaaaatggcccgttcttacttgagcatgtagctcttaatgagccagctgaagatcaggttctcgttagattggttgcaaccgggctgtgtcatacggatctggtttgtcgcgatcagcattatccggttccactaccgatggtatttgggcatgaaggggctggtgtggttgagcgggttgggtccgcggtcaaaaaggttcagccgggcgaccatgttgttttgacattttatacctgcgggagttgtgatgcttgtctttccggagaccctaccagttgtgcaaactcatttggccctaactttatggggcgctcggtaaccggggagtgcaccatccacgatcaccaaggggcagaggtgggagcaagcttttttgggcagtcctcctttgcgacatatgcgctatcttatgaacgtaacactgtgaaggttacaaaagacgtaccgcttgagttgcttgggcctcttggttgtggcattcaaactggcgcagggtctgttctgaatgcgcttaatccgccagcgggttctgctatcgcaatttttggtgctggggcagttggtctttcggccgtgatggctgccgttgtagcaggttgtaccaccatcatcgctgtcgacgttaaggaaaaccggctggaactagccagtgaacttggggcgacgcacattattaacccggccgctaacgatcccattgaggcgatcaaagagatattcgctgacggtgttccgtatgtattggagactagcggtttgcccgccgtgcttacgcaggcgatcctcagctctgctataggcggtgagatcggtattgtaggggcgccacctatgggggccacggtgcccgttgacattaacttcctgctattcaatcgtaagcttcgtggaatcgttgagggtcagtcgatctcggatattttcattcccaggctggtggagctttatcgccaggggaagtttccgtttgacaagctgattaagttttatccttttgatgaaatcaatcgagccgccgaagattcggaaaaaggcgtgacgcttaagccggtactccggattggttgaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61048_sequence/1
type: Sequence
persistent identity: BBa J61048 sequence
display id: BBa_J61048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K864601_sequence/1
type: Sequence
persistent identity: BBa K864601 sequence
display id: BBa_K864601_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtaacagagcattagcgcaaggtgatttttgtcttcttgcgctaatttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1840007_sequence/1
type: Sequence
persistent identity: BBa K1840007 sequence
display id: BBa_K1840007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagaggggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatctactagatggtttctaaaggtgaagaagacaacatggctatcatcaaagaatttatgcgtttcaaagttcacatggaaggttctgtgaacggtcacgaatttgaaatcgaaggtgaaggtgaaggtcgtccgtatgaaggcacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtctccgcagttcatgtacggttctaaagcgtatgttaaacacccggctgacatcccggactacctgaaactgtctttcccggaaggtttcaaatgggaacgtgttatgaactttgaagacggtggtgttgttaccgttacccaggactcttctctgcaagacggtgaatttatctacaaagttaaactgcgtggcaccaacttcccgtctgacggtccggttatgcagaaaaaaacgatgggttgggaagcgtcttctgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaacagcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacaaagctaaaaagccggttcaactgccgggtgcttacaacgtgaacatcaaactggacatcacctctcacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactctaccggcggtatggacgaactgtataaatgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2122100_sequence/1
type: Sequence
persistent identity: BBa K2122100 sequence
display id: BBa_K2122100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtccaagccccccgacctcctgctgcggctgctccggggcgccccaaggcagcgggtctgcaccctgttcatcatcggcttcaagttcacgtttttcgtctccatcatgatctactggcacgttgtgggagagcccaaggagaaagggcagctctataacctgccagcagagatcccctgccccaccttgacaccccccaccccaccctcccacggccccactccaggcaacatcttcttcctggagacttcagaccggaccaaccccaacttcctgttcatgtgctcggtggagtcggccgccagaactcaccccgaatcccacgtgctggtcctgatgaaagggcttccgggtggcaacgcctctctgccccggcacctgggcatctcacttctgagctgcttcccgaatgtccagatgctcccgctggacctgcgggagctgttccgggacacacccctggccgactggtacgcggccgtgcaggggcgctgggagccctacctgctgcccgtgctctccgacgcctccaggatcgcactcatgtggaagttcggcggcatctacctggacacggacttcattgttctcaagaacctgcggaacctgaccaacgtgctgggcacccagtcccgctacgtcctcaacggcgcgttcctggccttcgagcgccggcacgagttcatggcgctgtgcatgcgggacttcgtggaccactacaacggctggatctggggtcaccagggcccgcagctgctcacgcgggtcttcaagaagtggtgttccatccgcagcctggccgagagccgcgcctgccgcggcgtcaccaccctgccccctgaggccttctaccccatcccctggcaggactggaagaagtactttgaggacatcaaccccgaggagctgccgcggctgctcagtgccacctatgctgtccacgtgtggaacaagaagagccagggcacgcggttcgaggccacgtccagggcactgctggcccagctgcatgcccgctactgccccacgacgcacgaggccatgaaaatgtacttgtgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1009_sequence/1
type: Sequence
persistent identity: BBa B1009 sequence
display id: BBa_B1009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgccgaaaaccccgcccctgacagggcggggttttgccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K864501_sequence/1
type: Sequence
persistent identity: BBa K864501 sequence
display id: BBa_K864501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaataaagccctgagtttaaccgctcggggctttttgcgttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1004_sequence/1
type: Sequence
persistent identity: BBa B1004 sequence
display id: BBa_B1004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgccgaaaaccccgcttcggcggggttttgccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031112_sequence/1
type: Sequence
persistent identity: BBa K1031112 sequence
display id: BBa_K1031112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatggctagctcagtcctagggattatgctagctactagagaaagaggagaaatactagatgccgatcaagtacaagcctgaaatccagcactccgatttcaaggacctgaccaacctgatccacttccagagcacggaaggcaagatctggcttggcgaacaacgcatgctgttgctgcaagtttcagcaatggccagctttcgccgggaaatggtcaataccctgggcatcgaacgcgccaagggcttcttcctgcgccagggttaccagtccggcctgaaggatgccgaactggccaggaagcttagaccgaatgccagcgagtacgacatgttcctcgccggcccgcagctgcattcgctcaagggtctggtcaaggtccgccccaccgaggtcgatatcgacaaggaatgcgggcgcttctatgccgagatggagtggatcgactccttcgaggtggaaatctgccagaccgacctggggcagatgcaagacccggtgtgctggactctgctcggctacgcctgcgcctattcctcggcgttcatgggccgggaaatcatcttcaaggaagtaagctgccgcggctgcggcggcgacaagtgccgggtcattggcaagccggccgaagagtgggacgacgttgccagcttcaaacagtatttcaagaacgaccccatcatcgaggaactctacgagttgcaatcgcaactggtgtcgctgcgtaccaacctcgacaaacaggaaggccagtactacggcatcggtcagaccccggcctaccagaccgtgcgcaatatgatggacaaggccgcacagggcaaagtctcggtgctgctgcttggcgagaccggggtcggcaaggaggtcatcgcgcgtagcgtgcacctgcgcagcaaacgcgccgccgagccctttgtcgcggtgaactgtgcggcgatcccgccggacctgatcgagtccgaattgttcggcgtggaaaaaggcgccttcaccggcgccacccagtcacgcatgggccgcttcgagcgggccgacaagggcaccatcttccttgacgaggtgatcgaactcagcccgcgcgctcaggccagtctgctgcgcgtgctgcaagaaggcgagctggagcgagttggcgacaaccgcacgcgcaagatcgacgtaagggttatcgcagccacccacgaggacctggccgaagcggtcaaggccgggcgttttcgcgccgacctgtactaccggctgaacgttttcccggtggcgatcccggcgttgcgcgaacgccgcgaggacattccactgctggttgagcacttccttcagcgcttccaccaggagtacggcaagagaaccctcggcctttcagacaaagccctggaggcctgcctgcattacagttggccgggcaatatccgtgagctggagaacgtcatcgagcgcggcatcatcctcaccgatccgaacgaaagcatcagcgtgcaggcgctgttcccacgggcgccggaagagccgcagaccgccagcgagcgggtgtcgtcggacggcgtgctgattcagccaggcaatggccagggcagttggatcagccagttgttgagcagcggcctgagcctcgacgagatcgaggaaagcctgatgcgcgaagccatgcaacaggccaaccaaaacgtctccggtgccgcgcgcttgctcggcctaagccgaccggcactggcctatcggctgaagaaaatcggcatcgaaggctagactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1031223_sequence/1
type: Sequence
persistent identity: BBa K1031223 sequence
display id: BBa_K1031223_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcatttgctcaagcggccttgggcaattgatcaaatgcttaaaaagtctgcgcaagcgcggcttaatttcgctcgctccgatcattctaaaaattagaaacacattgaaaaacattaccttgaagtctgttttcagaccttggcacagccgttgcttgatgtcctgcgtactagagtcacacaggacactagaatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K731722_sequence/1
type: Sequence
persistent identity: BBa K731722 sequence
display id: BBa_K731722_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z