//terminator/single
type: Collection
persistent identity: terminator single
display id: terminator_single
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K801012
member: BBa_J61048
member: BBa_B0020
member: BBa_K731722
member: BBa_B1002
member: BBa_B1007
member: BBa_B0016
member: BBa_I51003
member: tADH1
member: BBa_B0011
member: BBa_B0050
member: BBa_K678012
member: BBa_K864600
member: trkD
member: BBa_B1005
member: BBa_B0062
member: BBa_B0053
member: BGH pA
member: BBa_B0023
member: TtrpC
member: BBa_B0060
member: BBa_C0440
member: BBa_K404108
member: BBa_B0013
member: Q0255 term
member: BBa_B0055
member: BBa_K780000
member: BBa_B0012
member: BBa_B1010
member: BBa_Y1015
member: BBa_K678039
member: BBa_B1006
member: BBa_B0063
member: BBa_B1001
member: COX3 termi
member: BBa_B0054
member: hGH pA
member: T22
member: BBa_B0061
member: BBa_B1008
member: BBa_K110012
member: BBa_K801011
member: BBa_B0010
member: BBa_K187025
member: T7stop
member: BBa_B1009
member: BBa_K864601
member: BBa_B1004
member: BBa_K404116
member: BBa_K678037
member: BBa_B0022
member: BBa_B0052
member: BBa_B0021
member: BBa_B1003
member: BBa_J52016
member: BBa_K678038
member: BBa_B0051
member: BBa_K392003

BBa_B0062
type: ComponentDefinition
persistent identity: BBa B0062
display id: BBa_B0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (Reverse B0052)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0062_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318637
display id: annotation318637
version: 1
top level: BBa_B0062
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1

https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
type: Range
persistent identity: range318637
display id: range318637
version: 1
direction: direction/forward
top level: BBa_B0062
start: 14
end: 33
orientation: inline

BBa_K801012
type: ComponentDefinition
persistent identity: BBa K801012
display id: BBa_K801012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADH1 yeast terminator
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ADH1 terminator of ''S. cerevisiae''
mutable notes: -
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ADH1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801012_sequence/1

ADH1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202145
display id: annotation2202145
version: 1
top level: BBa_K801012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1

https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1
type: Range
persistent identity: range2202145
display id: range2202145
version: 1
direction: direction/forward
top level: BBa_K801012
start: 1
end: 349
orientation: inline

hGH pA
type: ComponentDefinition
persistent identity: BBa K678018
display id: BBa_K678018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hGH poly A, terminator for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: hGH poly A, terminator for mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid N-terminal pFLAG-CMV from Sigma-Aldrich
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: hGH
sequence: https://synbiohub.org/public/igem/BBa_K678018_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127350
display id: annotation2127350
version: 1
top level: hGH pA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678018/annotation2127350/range2127350/1

https://synbiohub.org/public/igem/BBa_K678018/annotation2127350/range2127350/1
type: Range
persistent identity: range2127350
display id: range2127350
version: 1
direction: direction/forward
top level: hGH pA
start: 1
end: 8
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2127351
display id: annotation2127351
version: 1
top level: hGH pA
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678018/annotation2127351/range2127351/1

https://synbiohub.org/public/igem/BBa_K678018/annotation2127351/range2127351/1
type: Range
persistent identity: range2127351
display id: range2127351
version: 1
direction: direction/forward
top level: hGH pA
start: 9
end: 635
orientation: inline

BBa_K780000
type: ComponentDefinition
persistent identity: BBa K780000
display id: BBa_K780000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator for Bacillus subtilis
creator: Nikodem Latocha
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our terminator is based on strong, Rho-independent terminator of gyrase gene, which is type II topoisomerase (EC 5.99.1.3). DNA gyrase uses the energy of ATP hydrolysis to introduce negative supercoils or relaxes positive supercoils. Negative DNA supercoiling is essential in vivo to compact the genome, relieve torsional strain during replication, and promote local melting for vital processes such as transcript initation by RNA polymerase. Gyrase gene is an housekeeping gene and because of that it must have efficient terminator.
mutable notes: -
mutable provenance: Terminator of gyrase (EC 5.99.1.3) gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K780000_sequence/1

BBa_K187025
type: ComponentDefinition
persistent identity: BBa K187025
display id: BBa_K187025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: terminator in pAB, BioBytes plasmid
creator: Julia Pon
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:07Z
discontinued: false
dominant: true
experience: experience/None
group u list: _286_
m user id: 0
owner id: 4048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a bidirectional, high efficiency terminator, in the BioBytes universal plasmid pAB.See the BioBytes RFC for details on the BioBytes assembly method.
mutable notes: A terminator with long sticky ends can be prepared from this plasmid and assembled downstream of a gene using the Biobytes method. The addition of one DNA segment with the BioBytes method takes only 20 minutes. This ability to easily 'mix and match' terminators and genes allows rapid testing of different terminator designs.
mutable provenance: The terminator is derived from part b1006. pAB is entered as part K187000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: XbaI
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_K187025_sequence/1

XbaI
type: SequenceAnnotation
persistent identity: annotation2051724
display id: annotation2051724
version: 1
top level: BBa_K187025
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187025/annotation2051724/range2051724/1

https://synbiohub.org/public/igem/BBa_K187025/annotation2051724/range2051724/1
type: Range
persistent identity: range2051724
display id: range2051724
version: 1
direction: direction/forward
top level: BBa_K187025
start: 10
end: 15
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2051725
display id: annotation2051725
version: 1
top level: BBa_K187025
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187025/annotation2051725/range2051725/1

https://synbiohub.org/public/igem/BBa_K187025/annotation2051725/range2051725/1
type: Range
persistent identity: range2051725
display id: range2051725
version: 1
direction: direction/forward
top level: BBa_K187025
start: 53
end: 58
orientation: inline

BBa_B0016
type: ComponentDefinition
persistent identity: BBa B0016
display id: BBa_B0016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (T7 RNAP specific, T_Phi)
creator: Sri Kosuri
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: T7 gene 1 specific transcriptional terminator
mutable provenance: Sri Kosuri
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0016_sequence/1

BBa_J52016
type: ComponentDefinition
persistent identity: BBa J52016
display id: BBa_J52016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eukaryotic -- derived from SV40 early poly A signal sequence
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: It is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_J52016_sequence/1

terminator
type: SequenceAnnotation
persistent identity: annotation1902869
display id: annotation1902869
version: 1
top level: BBa_J52016
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52016/annotation1902869/range1902869/1

https://synbiohub.org/public/igem/BBa_J52016/annotation1902869/range1902869/1
type: Range
persistent identity: range1902869
display id: range1902869
version: 1
direction: direction/forward
top level: BBa_J52016
start: 1
end: 238
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1902870
display id: annotation1902870
version: 1
top level: BBa_J52016
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J52016/annotation1902870/range1902870/1

https://synbiohub.org/public/igem/BBa_J52016/annotation1902870/range1902870/1
type: Range
persistent identity: range1902870
display id: range1902870
version: 1
direction: direction/forward
top level: BBa_J52016
start: 1
end: 238
orientation: inline

BBa_B0060
type: ComponentDefinition
persistent identity: BBa B0060
display id: BBa_B0060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (Reverse B0050)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Reverse Bi-directional terminator from B0050 (derived from pBR322).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0060_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318622
display id: annotation318622
version: 1
top level: BBa_B0060
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0060/annotation318622/range318622/1

https://synbiohub.org/public/igem/BBa_B0060/annotation318622/range318622/1
type: Range
persistent identity: range318622
display id: range318622
version: 1
direction: direction/forward
top level: BBa_B0060
start: 10
end: 25
orientation: inline

BBa_Y1015
type: ComponentDefinition
persistent identity: BBa Y1015
display id: BBa_Y1015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CycE1
creator: Samantha Sutton
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_Y1015_sequence/1

BGH pA
type: ComponentDefinition
persistent identity: BBa K678019
display id: BBa_K678019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BGH poly A, mammalian terminator
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BGH poly A, terminator for use in mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: BGH
sequence: https://synbiohub.org/public/igem/BBa_K678019_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127352
display id: annotation2127352
version: 1
top level: BGH pA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678019/annotation2127352/range2127352/1

https://synbiohub.org/public/igem/BBa_K678019/annotation2127352/range2127352/1
type: Range
persistent identity: range2127352
display id: range2127352
version: 1
direction: direction/forward
top level: BGH pA
start: 1
end: 8
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2127353
display id: annotation2127353
version: 1
top level: BGH pA
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678019/annotation2127353/range2127353/1

https://synbiohub.org/public/igem/BBa_K678019/annotation2127353/range2127353/1
type: Range
persistent identity: range2127353
display id: range2127353
version: 1
direction: direction/forward
top level: BGH pA
start: 9
end: 233
orientation: inline

BBa_J61048
type: ComponentDefinition
persistent identity: BBa J61048
display id: BBa_J61048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [rnpB-T1] Terminator
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: bob
mutable notes: bob
mutable provenance: bob
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_J61048_sequence/1

BBa_I51003
type: ComponentDefinition
persistent identity: BBa I51003
display id: BBa_I51003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: Drew Endy
created: 2005-12-01T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _47_
m user id: 0
owner id: 52
owning group id: 47
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Super duper part!
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: foo binding site
sequence: https://synbiohub.org/public/igem/BBa_I51003_sequence/1

foo binding site
type: SequenceAnnotation
persistent identity: annotation1756359
display id: annotation1756359
version: 1
top level: BBa_I51003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I51003/annotation1756359/range1756359/1

https://synbiohub.org/public/igem/BBa_I51003/annotation1756359/range1756359/1
type: Range
persistent identity: range1756359
display id: range1756359
version: 1
direction: direction/forward
top level: BBa_I51003
start: 10
end: 50
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transcriptional terminator
creator: Reshma Shetty
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
direction: direction/forward
top level: BBa_B0054
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
direction: direction/forward
top level: BBa_B0054
start: 11
end: 42
orientation: inline

BBa_B1005
type: ComponentDefinition
persistent identity: BBa B1005
display id: BBa_B1005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, small, %T~=25%
creator: Haiyao Huang
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~=25% * 6bp stem, 4nt loop * Bidirectional, estimated reverse %T~<10%
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 3 residues. Designed to be a terminator with low efficiency.
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: stem loop
sequence annotation: B1005
sequence: https://synbiohub.org/public/igem/BBa_B1005_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1898427
display id: annotation1898427
version: 1
top level: BBa_B1005
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898427/range1898427/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898427/range1898427/1
type: Range
persistent identity: range1898427
display id: range1898427
version: 1
direction: direction/reverse
top level: BBa_B1005
start: 7
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898426
display id: annotation1898426
version: 1
top level: BBa_B1005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898426/range1898426/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898426/range1898426/1
type: Range
persistent identity: range1898426
display id: range1898426
version: 1
direction: direction/forward
top level: BBa_B1005
start: 10
end: 25
orientation: inline

B1005
type: SequenceAnnotation
persistent identity: annotation1898424
display id: annotation1898424
version: 1
top level: BBa_B1005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898424/range1898424/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898424/range1898424/1
type: Range
persistent identity: range1898424
display id: range1898424
version: 1
direction: direction/forward
top level: BBa_B1005
start: 1
end: 34
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898425
display id: annotation1898425
version: 1
top level: BBa_B1005
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898425/range1898425/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898425/range1898425/1
type: Range
persistent identity: range1898425
display id: range1898425
version: 1
direction: direction/forward
top level: BBa_B1005
start: 26
end: 28
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: Reshma Shetty
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to bacteriophage T3
sequence annotation: stem loop from bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
direction: direction/reverse
top level: BBa_B0055
start: 28
end: 66
orientation: inline

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
direction: direction/reverse
top level: BBa_B0055
start: 36
end: 57
orientation: inline

BBa_B0020
type: ComponentDefinition
persistent identity: BBa B0020
display id: BBa_B0020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (Reverse B0010)
creator: Caitlin Conboy
created: 2003-10-07T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop (B0010), cloned onto the antisense strand.
mutable provenance: Antiquity. thought to be T1 transcriptional terminator from E. coli RRNB operon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0020_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation354342
display id: annotation354342
version: 1
top level: BBa_B0020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0020/annotation354342/range354342/1

https://synbiohub.org/public/igem/BBa_B0020/annotation354342/range354342/1
type: Range
persistent identity: range354342
display id: range354342
version: 1
direction: direction/forward
top level: BBa_B0020
start: 27
end: 70
orientation: inline

BBa_B0061
type: ComponentDefinition
persistent identity: BBa B0061
display id: BBa_B0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (Reverse B0051)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Reverse of bi-directional terminator B0051. (E.coli derived, genbank U24206 bases 2694-2727. between converging yciA and tonA genes.)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0061_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318635
display id: annotation318635
version: 1
top level: BBa_B0061
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0061/annotation318635/range318635/1

https://synbiohub.org/public/igem/BBa_B0061/annotation318635/range318635/1
type: Range
persistent identity: range318635
display id: range318635
version: 1
direction: direction/forward
top level: BBa_B0061
start: 10
end: 25
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxICDABEG (+/-)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
direction: direction/forward
top level: BBa_B0011
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
direction: direction/forward
top level: BBa_B0011
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphageT7
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcription terminator for the E.coli RNA polymerase.
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: T7 TE
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
direction: direction/forward
top level: BBa_B0012
start: 1
end: 41
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
direction: direction/forward
top level: BBa_B0012
start: 28
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
direction: direction/forward
top level: BBa_B0012
start: 8
end: 27
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
direction: direction/forward
top level: BBa_B0012
start: 34
end: 34
orientation: inline

BBa_K110012
type: ComponentDefinition
persistent identity: BBa K110012
display id: BBa_K110012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: STE2 terminator
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the exact region between the end of the ORF of STE2-W and the beginning of BST1-C
mutable notes: This sequence was taken from the yeast genome
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Terminator
sequence: https://synbiohub.org/public/igem/BBa_K110012_sequence/1

Terminator
type: SequenceAnnotation
persistent identity: annotation1994877
display id: annotation1994877
version: 1
top level: BBa_K110012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K110012/annotation1994877/range1994877/1

https://synbiohub.org/public/igem/BBa_K110012/annotation1994877/range1994877/1
type: Range
persistent identity: range1994877
display id: range1994877
version: 1
direction: direction/forward
top level: BBa_K110012
start: 1
end: 123
orientation: inline

BBa_K864601
type: ComponentDefinition
persistent identity: BBa K864601
display id: BBa_K864601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda t1 transcriptional terminator
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lambda t1 is a strong transcriptional terminator with 99% efficiency in vivo.
mutable notes: -
mutable provenance: Gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: lambda t1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K864601_sequence/1

lambda t1 terminator
type: SequenceAnnotation
persistent identity: annotation2197328
display id: annotation2197328
version: 1
top level: BBa_K864601
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864601/annotation2197328/range2197328/1

https://synbiohub.org/public/igem/BBa_K864601/annotation2197328/range2197328/1
type: Range
persistent identity: range2197328
display id: range2197328
version: 1
direction: direction/forward
top level: BBa_K864601
start: 1
end: 53
orientation: inline

BBa_K678012
type: ComponentDefinition
persistent identity: BBa K678012
display id: BBa_K678012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40 poly A, terminator for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SV40 poly A, terminator for use in mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: SV40
sequence annotation: Linker 4
sequence: https://synbiohub.org/public/igem/BBa_K678012_sequence/1

SV40
type: SequenceAnnotation
persistent identity: annotation2127355
display id: annotation2127355
version: 1
top level: BBa_K678012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678012/annotation2127355/range2127355/1

https://synbiohub.org/public/igem/BBa_K678012/annotation2127355/range2127355/1
type: Range
persistent identity: range2127355
display id: range2127355
version: 1
direction: direction/forward
top level: BBa_K678012
start: 9
end: 139
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127354
display id: annotation2127354
version: 1
top level: BBa_K678012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678012/annotation2127354/range2127354/1

https://synbiohub.org/public/igem/BBa_K678012/annotation2127354/range2127354/1
type: Range
persistent identity: range2127354
display id: range2127354
version: 1
direction: direction/forward
top level: BBa_K678012
start: 1
end: 8
orientation: inline

BBa_B1004
type: ComponentDefinition
persistent identity: BBa B1004
display id: BBa_B1004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, small, %T~=55)
creator: Haiyao Huang
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Artificial terminator, estimated %T~=55 * 6bp stem, 4nt loop * Bidirectional, estimated reverse %T~<20
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 4 residues.
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem loop
sequence annotation: B1004
sequence annotation: PolyA
sequence: https://synbiohub.org/public/igem/BBa_B1004_sequence/1

stem loop
type: SequenceAnnotation
persistent identity: annotation1898421
display id: annotation1898421
version: 1
top level: BBa_B1004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898421/range1898421/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898421/range1898421/1
type: Range
persistent identity: range1898421
display id: range1898421
version: 1
direction: direction/forward
top level: BBa_B1004
start: 10
end: 25
orientation: inline

B1004
type: SequenceAnnotation
persistent identity: annotation1898420
display id: annotation1898420
version: 1
top level: BBa_B1004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898420/range1898420/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898420/range1898420/1
type: Range
persistent identity: range1898420
display id: range1898420
version: 1
direction: direction/forward
top level: BBa_B1004
start: 1
end: 34
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898422
display id: annotation1898422
version: 1
top level: BBa_B1004
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898422/range1898422/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898422/range1898422/1
type: Range
persistent identity: range1898422
display id: range1898422
version: 1
direction: direction/reverse
top level: BBa_B1004
start: 6
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898423
display id: annotation1898423
version: 1
top level: BBa_B1004
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898423/range1898423/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898423/range1898423/1
type: Range
persistent identity: range1898423
display id: range1898423
version: 1
direction: direction/forward
top level: BBa_B1004
start: 26
end: 29
orientation: inline

BBa_B1002
type: ComponentDefinition
persistent identity: BBa B1002
display id: BBa_B1002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, small, %T~=85%)
creator: Haiyao Huang
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artifical terminator, estimated %T~=85
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Poly A tail
sequence annotation: stem loop
sequence annotation: B1002
sequence: https://synbiohub.org/public/igem/BBa_B1002_sequence/1

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898414
display id: annotation1898414
version: 1
top level: BBa_B1002
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
type: Range
persistent identity: range1898414
display id: range1898414
version: 1
direction: direction/forward
top level: BBa_B1002
start: 25
end: 31
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898413
display id: annotation1898413
version: 1
top level: BBa_B1002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
type: Range
persistent identity: range1898413
display id: range1898413
version: 1
direction: direction/forward
top level: BBa_B1002
start: 10
end: 25
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898415
display id: annotation1898415
version: 1
top level: BBa_B1002
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
type: Range
persistent identity: range1898415
display id: range1898415
version: 1
direction: direction/reverse
top level: BBa_B1002
start: 4
end: 9
orientation: inline

B1002
type: SequenceAnnotation
persistent identity: annotation1898412
display id: annotation1898412
version: 1
top level: BBa_B1002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
type: Range
persistent identity: range1898412
display id: range1898412
version: 1
direction: direction/forward
top level: BBa_B1002
start: 1
end: 34
orientation: inline

BBa_K801011
type: ComponentDefinition
persistent identity: BBa K801011
display id: BBa_K801011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEF1 yeast terminator
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TEF1 terminator of ''S. cervisiae''.
mutable notes: Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: TEF1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801011_sequence/1

TEF1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202142
display id: annotation2202142
version: 1
top level: BBa_K801011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801011/annotation2202142/range2202142/1

https://synbiohub.org/public/igem/BBa_K801011/annotation2202142/range2202142/1
type: Range
persistent identity: range2202142
display id: range2202142
version: 1
direction: direction/forward
top level: BBa_K801011
start: 1
end: 507
orientation: inline

BBa_C0440
type: ComponentDefinition
persistent identity: BBa C0440
display id: BBa_C0440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (bgl)
creator: T. Senthil
created: 2005-10-28T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
group u list: _44_
m user id: 0
owner id: 439
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator in Bgl operon that is antiterminated by bglG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: bgl terminator
sequence: https://synbiohub.org/public/igem/BBa_C0440_sequence/1

bgl terminator
type: SequenceAnnotation
persistent identity: annotation1744294
display id: annotation1744294
version: 1
top level: BBa_C0440
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_C0440/annotation1744294/range1744294/1

https://synbiohub.org/public/igem/BBa_C0440/annotation1744294/range1744294/1
type: Range
persistent identity: range1744294
display id: range1744294
version: 1
direction: direction/forward
top level: BBa_C0440
start: 68
end: 119
orientation: inline

TtrpC
type: ComponentDefinition
persistent identity: BBa K678036
display id: BBa_K678036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: trpC terminator for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TtrpC is a terminator of tryptophan synthase of Aspergillus nidulans.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From the plasmid p71 from the Center for Microbial Biotechnology at the Technical University of Denmark plasmid collection.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: TtrpC terminator
sequence: https://synbiohub.org/public/igem/BBa_K678036_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127403
display id: annotation2127403
version: 1
top level: TtrpC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678036/annotation2127403/range2127403/1

https://synbiohub.org/public/igem/BBa_K678036/annotation2127403/range2127403/1
type: Range
persistent identity: range2127403
display id: range2127403
version: 1
direction: direction/forward
top level: TtrpC
start: 1
end: 8
orientation: inline

TtrpC terminator
type: SequenceAnnotation
persistent identity: annotation2127404
display id: annotation2127404
version: 1
top level: TtrpC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678036/annotation2127404/range2127404/1

https://synbiohub.org/public/igem/BBa_K678036/annotation2127404/range2127404/1
type: Range
persistent identity: range2127404
display id: range2127404
version: 1
direction: direction/forward
top level: TtrpC
start: 9
end: 759
orientation: inline

BBa_B0023
type: ComponentDefinition
persistent identity: BBa B0023
display id: BBa_B0023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphage T7, reversed
creator: Caitlin Conboy
created: 2003-10-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the reverse of terminator B0013, a bacteriophage T7 terminator which was engineered to be bidirectional. Contains a poly A insertion upstream of a 20 bp stem-loop.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0023_sequence/1

BBa_B0052
type: ComponentDefinition
persistent identity: BBa B0052
display id: BBa_B0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (rrnC)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator from the E.coli ribosonal RNA rrnC operon. This terminator occurs in the reverse orientation upstream of the multiple cloning site of the pSB**1-pSB**3 series of plasmids.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0052_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318601
display id: annotation318601
version: 1
top level: BBa_B0052
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0052/annotation318601/range318601/1

https://synbiohub.org/public/igem/BBa_B0052/annotation318601/range318601/1
type: Range
persistent identity: range318601
display id: range318601
version: 1
direction: direction/forward
top level: BBa_B0052
start: 9
end: 28
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~>90)
creator: Haiyao Huang
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: modified thr terminator
sequence annotation: PolyA
sequence annotation: B1006
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
direction: direction/forward
top level: BBa_B1006
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
direction: direction/reverse
top level: BBa_B1006
start: 1
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
direction: direction/forward
top level: BBa_B1006
start: 32
end: 39
orientation: inline

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
direction: direction/forward
top level: BBa_B1006
start: 1
end: 39
orientation: inline

T7stop
type: ComponentDefinition
persistent identity: BBa J18913
display id: BBa_J18913
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 terminator (incl. STOP)
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
mutable notes: * taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
mutable provenance: pET3a expression plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J18913_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2065587
display id: annotation2065587
version: 1
top level: T7stop
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1

https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
type: Range
persistent identity: range2065587
display id: range2065587
version: 1
direction: direction/forward
top level: T7stop
start: 1
end: 6
orientation: inline

trkD
type: ComponentDefinition
persistent identity: BBa K629005
display id: BBa_K629005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
creator: Zilong WANG, Yi ZHENG
created: 2011-10-03T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
mutable notes: none
mutable provenance: ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTAATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGCTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTCCCTATTCGTCTGGCGTGGTTCACTGTCGTATTACCTTCCTTAACTCTTAATTACTTCGGCCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTTTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATCGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rev primer
sequence annotation: fwd primer
sequence annotation: terminator
sequence annotation: start
sequence annotation: TrkD
sequence: https://synbiohub.org/public/igem/BBa_K629005_sequence/1

rev primer
type: SequenceAnnotation
persistent identity: annotation2157757
display id: annotation2157757
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1
type: Range
persistent identity: range2157757
display id: range2157757
version: 1
direction: direction/reverse
top level: trkD
start: 1849
end: 1869
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2157755
display id: annotation2157755
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1
type: Range
persistent identity: range2157755
display id: range2157755
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 21
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2157758
display id: annotation2157758
version: 1
top level: trkD
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1
type: Range
persistent identity: range2157758
display id: range2157758
version: 1
direction: direction/forward
top level: trkD
start: 1867
end: 1869
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2157754
display id: annotation2157754
version: 1
top level: trkD
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1
type: Range
persistent identity: range2157754
display id: range2157754
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 3
orientation: inline

TrkD
type: SequenceAnnotation
persistent identity: annotation2157756
display id: annotation2157756
version: 1
top level: trkD
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1
type: Range
persistent identity: range2157756
display id: range2157756
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 1869
orientation: inline

BBa_B1003
type: ComponentDefinition
persistent identity: BBa B1003
display id: BBa_B1003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, small, %T~=80)
creator: Haiyao Huang
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artifical terminator, estimated %T~=80 6bp stem, 4nt loop Bidirectional, estimated reverse %T=40
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 5 residues.
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem loop
sequence annotation: PolyA
sequence annotation: B1003
sequence: https://synbiohub.org/public/igem/BBa_B1003_sequence/1

stem loop
type: SequenceAnnotation
persistent identity: annotation1898417
display id: annotation1898417
version: 1
top level: BBa_B1003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898417/range1898417/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898417/range1898417/1
type: Range
persistent identity: range1898417
display id: range1898417
version: 1
direction: direction/forward
top level: BBa_B1003
start: 10
end: 25
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898419
display id: annotation1898419
version: 1
top level: BBa_B1003
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898419/range1898419/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898419/range1898419/1
type: Range
persistent identity: range1898419
display id: range1898419
version: 1
direction: direction/forward
top level: BBa_B1003
start: 26
end: 30
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898418
display id: annotation1898418
version: 1
top level: BBa_B1003
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898418/range1898418/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898418/range1898418/1
type: Range
persistent identity: range1898418
display id: range1898418
version: 1
direction: direction/reverse
top level: BBa_B1003
start: 5
end: 9
orientation: inline

B1003
type: SequenceAnnotation
persistent identity: annotation1898416
display id: annotation1898416
version: 1
top level: BBa_B1003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898416/range1898416/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898416/range1898416/1
type: Range
persistent identity: range1898416
display id: range1898416
version: 1
direction: direction/forward
top level: BBa_B1003
start: 1
end: 34
orientation: inline

BBa_B1008
type: ComponentDefinition
persistent identity: BBa B1008
display id: BBa_B1008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~=70)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~=70% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~=80%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 5 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pair
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1008
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1008_sequence/1

B1008
type: SequenceAnnotation
persistent identity: annotation1901005
display id: annotation1901005
version: 1
top level: BBa_B1008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901005/range1901005/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901005/range1901005/1
type: Range
persistent identity: range1901005
display id: range1901005
version: 1
direction: direction/forward
top level: BBa_B1008
start: 1
end: 40
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901007
display id: annotation1901007
version: 1
top level: BBa_B1008
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901007/range1901007/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901007/range1901007/1
type: Range
persistent identity: range1901007
display id: range1901007
version: 1
direction: direction/reverse
top level: BBa_B1008
start: 5
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901008
display id: annotation1901008
version: 1
top level: BBa_B1008
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901008/range1901008/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901008/range1901008/1
type: Range
persistent identity: range1901008
display id: range1901008
version: 1
direction: direction/forward
top level: BBa_B1008
start: 32
end: 36
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1901006
display id: annotation1901006
version: 1
top level: BBa_B1008
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901006/range1901006/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901006/range1901006/1
type: Range
persistent identity: range1901006
display id: range1901006
version: 1
direction: direction/forward
top level: BBa_B1008
start: 10
end: 31
orientation: inline

BBa_K392003
type: ComponentDefinition
persistent identity: BBa K392003
display id: BBa_K392003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast ADH1 terminator
creator: Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
created: 2010-10-09T11:00:00Z
modified: 2015-05-08T01:12:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _528_
m user id: 0
owner id: 6220
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Engineered 'cyc100 promoter' of Saccharomyces cerevisiae with Kozak sequence attached downstream.
mutable notes: Assembled by 3A method.
mutable provenance: Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K392003_sequence/1

tADH1
type: ComponentDefinition
persistent identity: BBa J63002
display id: BBa_J63002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADH1 terminator from S. cerevisiae
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ADH1 terminator from S. cerevisiae
mutable notes: starts with stop codon
mutable provenance: genomic DNA of S. cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ADH1 terminator
sequence: https://synbiohub.org/public/igem/BBa_J63002_sequence/1

ADH1 terminator
type: SequenceAnnotation
persistent identity: annotation1902851
display id: annotation1902851
version: 1
top level: tADH1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J63002/annotation1902851/range1902851/1

https://synbiohub.org/public/igem/BBa_J63002/annotation1902851/range1902851/1
type: Range
persistent identity: range1902851
display id: range1902851
version: 1
direction: direction/forward
top level: tADH1
start: 1
end: 225
orientation: inline

BBa_B0021
type: ComponentDefinition
persistent identity: BBa B0021
display id: BBa_B0021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxICDABEG (+/-), reversed
creator: Caitlin Conboy
created: 2003-10-13T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Bidirectional transcriptional terminator cloned in the reverse direction of B0011. 22 bp stem-loop
mutable notes: Cloned with primer-dimers into pSB1A2
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0021
sequence: https://synbiohub.org/public/igem/BBa_B0021_sequence/1

BBa_B0021
type: SequenceAnnotation
persistent identity: annotation7024
display id: annotation7024
version: 1
top level: BBa_B0021
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0021/annotation7024/range7024/1

https://synbiohub.org/public/igem/BBa_B0021/annotation7024/range7024/1
type: Range
persistent identity: range7024
display id: range7024
version: 1
direction: direction/forward
top level: BBa_B0021
start: 1
end: 46
orientation: inline

BBa_B0063
type: ComponentDefinition
persistent identity: BBa B0063
display id: BBa_B0063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (Reverse B0053)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Reverse of B0053, E.coli transcriptional terminator from the His operon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0063_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318639
display id: annotation318639
version: 1
top level: BBa_B0063
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0063/annotation318639/range318639/1

https://synbiohub.org/public/igem/BBa_B0063/annotation318639/range318639/1
type: Range
persistent identity: range318639
display id: range318639
version: 1
direction: direction/forward
top level: BBa_B0063
start: 15
end: 64
orientation: inline

BBa_B1009
type: ComponentDefinition
persistent identity: BBa B1009
display id: BBa_B1009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~=40%)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~>90% * 8bp stem, 6nt loop * Bidirectional, estimated reverse %T~>90%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 4 residues
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: B1009
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1009_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1901011
display id: annotation1901011
version: 1
top level: BBa_B1009
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901011/range1901011/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901011/range1901011/1
type: Range
persistent identity: range1901011
display id: range1901011
version: 1
direction: direction/reverse
top level: BBa_B1009
start: 6
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901012
display id: annotation1901012
version: 1
top level: BBa_B1009
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901012/range1901012/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901012/range1901012/1
type: Range
persistent identity: range1901012
display id: range1901012
version: 1
direction: direction/forward
top level: BBa_B1009
start: 32
end: 35
orientation: inline

B1009
type: SequenceAnnotation
persistent identity: annotation1901009
display id: annotation1901009
version: 1
top level: BBa_B1009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901009/range1901009/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901009/range1901009/1
type: Range
persistent identity: range1901009
display id: range1901009
version: 1
direction: direction/forward
top level: BBa_B1009
start: 1
end: 40
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1901010
display id: annotation1901010
version: 1
top level: BBa_B1009
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901010/range1901010/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901010/range1901010/1
type: Range
persistent identity: range1901010
display id: range1901010
version: 1
direction: direction/forward
top level: BBa_B1009
start: 10
end: 31
orientation: inline

BBa_K731722
type: ComponentDefinition
persistent identity: BBa K731722
display id: BBa_K731722
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T1 terminator from E. coli rrnB
creator: Anna Depetris, Giacomo Giacomelli
created: 2012-08-22T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12063
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the B0010 part extracted by PCR from the doulbe terminator B0012. We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use! The characterization has been done both with T7 and Coli RNApolymerases.
mutable notes: I don't have any design considerations, anyway it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.
mutable provenance: This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0010
sequence: https://synbiohub.org/public/igem/BBa_K731722_sequence/1

B0010
type: SequenceAnnotation
persistent identity: annotation2180631
display id: annotation2180631
version: 1
top level: BBa_K731722
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K731722/annotation2180631/range2180631/1

https://synbiohub.org/public/igem/BBa_K731722/annotation2180631/range2180631/1
type: Range
persistent identity: range2180631
display id: range2180631
version: 1
direction: direction/forward
top level: BBa_K731722
start: 1
end: 79
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T1 from E. coli rrnB
creator: Randy Rettberg
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
direction: direction/forward
top level: BBa_B0010
start: 12
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
direction: direction/forward
top level: BBa_B0010
start: 1
end: 80
orientation: inline

BBa_B1001
type: ComponentDefinition
persistent identity: BBa B1001
display id: BBa_B1001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artifical, small, %T~=90)
creator: Haiyao Huang
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T>90
mutable notes: Poly T tail of 9 residues for high %T
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA tail
sequence annotation: stem loop
sequence annotation: B1001
sequence annotation: Poly A tail
sequence: https://synbiohub.org/public/igem/BBa_B1001_sequence/1

PolyA tail
type: SequenceAnnotation
persistent identity: annotation1898381
display id: annotation1898381
version: 1
top level: BBa_B1001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898381/range1898381/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898381/range1898381/1
type: Range
persistent identity: range1898381
display id: range1898381
version: 1
direction: direction/reverse
top level: BBa_B1001
start: 1
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898379
display id: annotation1898379
version: 1
top level: BBa_B1001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898379/range1898379/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898379/range1898379/1
type: Range
persistent identity: range1898379
display id: range1898379
version: 1
direction: direction/forward
top level: BBa_B1001
start: 10
end: 25
orientation: inline

B1001
type: SequenceAnnotation
persistent identity: annotation1898382
display id: annotation1898382
version: 1
top level: BBa_B1001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898382/range1898382/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898382/range1898382/1
type: Range
persistent identity: range1898382
display id: range1898382
version: 1
direction: direction/forward
top level: BBa_B1001
start: 1
end: 34
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898380
display id: annotation1898380
version: 1
top level: BBa_B1001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898380/range1898380/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898380/range1898380/1
type: Range
persistent identity: range1898380
display id: range1898380
version: 1
direction: direction/reverse
top level: BBa_B1001
start: 26
end: 34
orientation: inline

BBa_B0051
type: ComponentDefinition
persistent identity: BBa B0051
display id: BBa_B0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (yciA/tonA, +/-)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Bi-directional Terminator from pSMART HCKAN. E.coli derived, genbank U24206 bases 2694-2727 between yciA and tonA genes (converging transcripts)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0051_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318600
display id: annotation318600
version: 1
top level: BBa_B0051
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0051/annotation318600/range318600/1

https://synbiohub.org/public/igem/BBa_B0051/annotation318600/range318600/1
type: Range
persistent identity: range318600
display id: range318600
version: 1
direction: direction/forward
top level: BBa_B0051
start: 11
end: 26
orientation: inline

BBa_K678038
type: ComponentDefinition
persistent identity: BBa K678038
display id: BBa_K678038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T2-motni, terminator for Aspergillus niger
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T2-motni is a terminator for use in Aspergillus niger
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Aspergillus niger genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: T2-motni terminator
sequence: https://synbiohub.org/public/igem/BBa_K678038_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127407
display id: annotation2127407
version: 1
top level: BBa_K678038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678038/annotation2127407/range2127407/1

https://synbiohub.org/public/igem/BBa_K678038/annotation2127407/range2127407/1
type: Range
persistent identity: range2127407
display id: range2127407
version: 1
direction: direction/forward
top level: BBa_K678038
start: 1
end: 8
orientation: inline

T2-motni terminator
type: SequenceAnnotation
persistent identity: annotation2127408
display id: annotation2127408
version: 1
top level: BBa_K678038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678038/annotation2127408/range2127408/1

https://synbiohub.org/public/igem/BBa_K678038/annotation2127408/range2127408/1
type: Range
persistent identity: range2127408
display id: range2127408
version: 1
direction: direction/forward
top level: BBa_K678038
start: 9
end: 990
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (His)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator from the E.coli His operon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
direction: direction/forward
top level: BBa_B0053
start: 9
end: 43
orientation: inline

COX3 termi
type: ComponentDefinition
persistent identity: BBa K809005
display id: BBa_K809005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator of Yeast mitochondrial gene COX3
creator: Xiaopeng Xu
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the terminator region for COX3 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
mutable notes: We modelled the RNA secondary structure of COX3 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
mutable provenance: This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region. http://www.yeastgenome.org/
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K809005_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2194193
display id: annotation2194193
version: 1
top level: COX3 termi
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K809005/annotation2194193/range2194193/1

https://synbiohub.org/public/igem/BBa_K809005/annotation2194193/range2194193/1
type: Range
persistent identity: range2194193
display id: range2194193
version: 1
direction: direction/forward
top level: COX3 termi
start: 16
end: 34
orientation: inline

BBa_B0050
type: ComponentDefinition
persistent identity: BBa B0050
display id: BBa_B0050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (pBR322, +/-)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Bi-directional Terminator derived from pBR322.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0050_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318591
display id: annotation318591
version: 1
top level: BBa_B0050
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0050/annotation318591/range318591/1

https://synbiohub.org/public/igem/BBa_B0050/annotation318591/range318591/1
type: Range
persistent identity: range318591
display id: range318591
version: 1
direction: direction/forward
top level: BBa_B0050
start: 8
end: 24
orientation: inline

BBa_B0022
type: ComponentDefinition
persistent identity: BBa B0022
display id: BBa_B0022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphageT7, reversed
creator: Caitlin Conboy
created: 2003-10-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Uni-directional terminator. Reverse of part B0012. 20 bp stem-loop.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0022_sequence/1

Q0255 term
type: ComponentDefinition
persistent identity: BBa K809006
display id: BBa_K809006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator of Yeast mitochondrial gene Q0255
creator: Xiaopeng Xu
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the terminator region for Q0255 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
mutable notes: This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region. http://www.yeastgenome.org/
mutable provenance: We modelled the RNA secondary structure of Q0255 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K809006_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2194556
display id: annotation2194556
version: 1
top level: Q0255 term
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K809006/annotation2194556/range2194556/1

https://synbiohub.org/public/igem/BBa_K809006/annotation2194556/range2194556/1
type: Range
persistent identity: range2194556
display id: range2194556
version: 1
direction: direction/forward
top level: Q0255 term
start: 16
end: 37
orientation: inline

BBa_K404116
type: ComponentDefinition
persistent identity: BBa K404116
display id: BBa_K404116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: hGH
sequence annotation: right ITR
sequence: https://synbiohub.org/public/igem/BBa_K404116_sequence/1

hGH
type: SequenceAnnotation
persistent identity: annotation2093227
display id: annotation2093227
version: 1
top level: BBa_K404116
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404116/annotation2093227/range2093227/1

https://synbiohub.org/public/igem/BBa_K404116/annotation2093227/range2093227/1
type: Range
persistent identity: range2093227
display id: range2093227
version: 1
direction: direction/forward
top level: BBa_K404116
start: 1
end: 482
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093229
display id: annotation2093229
version: 1
top level: BBa_K404116
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404116/annotation2093229/range2093229/1

https://synbiohub.org/public/igem/BBa_K404116/annotation2093229/range2093229/1
type: Range
persistent identity: range2093229
display id: range2093229
version: 1
direction: direction/forward
top level: BBa_K404116
start: 495
end: 632
orientation: inline

BBa_B0013
type: ComponentDefinition
persistent identity: BBa B0013
display id: BBa_B0013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphage T7 (+/-)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream. Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: polya
sequence annotation: C
sequence annotation: ''
sequence annotation: T7 TE
sequence annotation: BBa_B0013
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0013_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation1698
display id: annotation1698
version: 1
top level: BBa_B0013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1
type: Range
persistent identity: range1698
display id: range1698
version: 1
direction: direction/forward
top level: BBa_B0013
start: 34
end: 47
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1695
display id: annotation1695
version: 1
top level: BBa_B0013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1
type: Range
persistent identity: range1695
display id: range1695
version: 1
direction: direction/forward
top level: BBa_B0013
start: 37
end: 37
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1696
display id: annotation1696
version: 1
top level: BBa_B0013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1
type: Range
persistent identity: range1696
display id: range1696
version: 1
direction: direction/forward
top level: BBa_B0013
start: 10
end: 10
orientation: inline

''
type: SequenceAnnotation
persistent identity: annotation1697
display id: annotation1697
version: 1
top level: BBa_B0013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1
type: Range
persistent identity: range1697
display id: range1697
version: 1
direction: direction/forward
top level: BBa_B0013
start: 1
end: 6
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1691
display id: annotation1691
version: 1
top level: BBa_B0013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1
type: Range
persistent identity: range1691
display id: range1691
version: 1
direction: direction/forward
top level: BBa_B0013
start: 14
end: 33
orientation: inline

BBa_B0013
type: SequenceAnnotation
persistent identity: annotation7021
display id: annotation7021
version: 1
top level: BBa_B0013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1

https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1
type: Range
persistent identity: range7021
display id: range7021
version: 1
direction: direction/forward
top level: BBa_B0013
start: 1
end: 47
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1692
display id: annotation1692
version: 1
top level: BBa_B0013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1
type: Range
persistent identity: range1692
display id: range1692
version: 1
direction: direction/forward
top level: BBa_B0013
start: 40
end: 40
orientation: inline

BBa_K404108
type: ComponentDefinition
persistent identity: BBa K404108
display id: BBa_K404108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hGH terminator
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: hGH terminator
sequence: https://synbiohub.org/public/igem/BBa_K404108_sequence/1

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2093173
display id: annotation2093173
version: 1
top level: BBa_K404108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404108/annotation2093173/range2093173/1

https://synbiohub.org/public/igem/BBa_K404108/annotation2093173/range2093173/1
type: Range
persistent identity: range2093173
display id: range2093173
version: 1
direction: direction/forward
top level: BBa_K404108
start: 1
end: 481
orientation: inline

BBa_K678037
type: ComponentDefinition
persistent identity: BBa K678037
display id: BBa_K678037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T1-motni, terminator for Aspergillus niger
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T1-motni is terminator that works in the fungus Aspergillus niger
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Aspergillus niger genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: T1-motni terminator
sequence: https://synbiohub.org/public/igem/BBa_K678037_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127406
display id: annotation2127406
version: 1
top level: BBa_K678037
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678037/annotation2127406/range2127406/1

https://synbiohub.org/public/igem/BBa_K678037/annotation2127406/range2127406/1
type: Range
persistent identity: range2127406
display id: range2127406
version: 1
direction: direction/forward
top level: BBa_K678037
start: 1
end: 8
orientation: inline

T1-motni terminator
type: SequenceAnnotation
persistent identity: annotation2127405
display id: annotation2127405
version: 1
top level: BBa_K678037
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678037/annotation2127405/range2127405/1

https://synbiohub.org/public/igem/BBa_K678037/annotation2127405/range2127405/1
type: Range
persistent identity: range2127405
display id: range2127405
version: 1
direction: direction/forward
top level: BBa_K678037
start: 9
end: 1006
orientation: inline

BBa_B1007
type: ComponentDefinition
persistent identity: BBa B1007
display id: BBa_B1007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~=80)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~=80% # 8bp stem, 6nt loop # Bidirectional, estimated reverse %T~>90%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 6 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: modified thr
sequence annotation: B1007
sequence: https://synbiohub.org/public/igem/BBa_B1007_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1900958
display id: annotation1900958
version: 1
top level: BBa_B1007
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900958/range1900958/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900958/range1900958/1
type: Range
persistent identity: range1900958
display id: range1900958
version: 1
direction: direction/forward
top level: BBa_B1007
start: 32
end: 37
orientation: inline

modified thr
type: SequenceAnnotation
persistent identity: annotation1900956
display id: annotation1900956
version: 1
top level: BBa_B1007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900956/range1900956/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900956/range1900956/1
type: Range
persistent identity: range1900956
display id: range1900956
version: 1
direction: direction/forward
top level: BBa_B1007
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1900957
display id: annotation1900957
version: 1
top level: BBa_B1007
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900957/range1900957/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900957/range1900957/1
type: Range
persistent identity: range1900957
display id: range1900957
version: 1
direction: direction/reverse
top level: BBa_B1007
start: 4
end: 9
orientation: inline

B1007
type: SequenceAnnotation
persistent identity: annotation1900955
display id: annotation1900955
version: 1
top level: BBa_B1007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900955/range1900955/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900955/range1900955/1
type: Range
persistent identity: range1900955
display id: range1900955
version: 1
direction: direction/forward
top level: BBa_B1007
start: 1
end: 40
orientation: inline

BBa_K864600
type: ComponentDefinition
persistent identity: BBa K864600
display id: BBa_K864600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T0 (21 imm) transcriptional terminator
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The T0 transcriptional terminator terminates transcription with 80-90% efficiency.
mutable notes: -
mutable provenance: Gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: T0 terminator
sequence: https://synbiohub.org/public/igem/BBa_K864600_sequence/1

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2197273
display id: annotation2197273
version: 1
top level: BBa_K864600
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864600/annotation2197273/range2197273/1

https://synbiohub.org/public/igem/BBa_K864600/annotation2197273/range2197273/1
type: Range
persistent identity: range2197273
display id: range2197273
version: 1
direction: direction/forward
top level: BBa_K864600
start: 1
end: 52
orientation: inline

BBa_K678039
type: ComponentDefinition
persistent identity: BBa K678039
display id: BBa_K678039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T3-motni, terminator for Aspergillus niger
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T3-motni is a terminator for use in Aspergillus niger.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Aspergillus niger genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: T3-motni terminator
sequence: https://synbiohub.org/public/igem/BBa_K678039_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127409
display id: annotation2127409
version: 1
top level: BBa_K678039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678039/annotation2127409/range2127409/1

https://synbiohub.org/public/igem/BBa_K678039/annotation2127409/range2127409/1
type: Range
persistent identity: range2127409
display id: range2127409
version: 1
direction: direction/forward
top level: BBa_K678039
start: 1
end: 8
orientation: inline

T3-motni terminator
type: SequenceAnnotation
persistent identity: annotation2127410
display id: annotation2127410
version: 1
top level: BBa_K678039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678039/annotation2127410/range2127410/1

https://synbiohub.org/public/igem/BBa_K678039/annotation2127410/range2127410/1
type: Range
persistent identity: range2127410
display id: range2127410
version: 1
direction: direction/forward
top level: BBa_K678039
start: 9
end: 889
orientation: inline

BBa_B1010
type: ComponentDefinition
persistent identity: BBa B1010
display id: BBa_B1010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terninator (artificial, large, %T~<10)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~<10% 8bp stem, 6nt loop Bidirectional, estimated reverse %T~=40%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 3 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: B1010
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1010_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1901016
display id: annotation1901016
version: 1
top level: BBa_B1010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901016/range1901016/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901016/range1901016/1
type: Range
persistent identity: range1901016
display id: range1901016
version: 1
direction: direction/forward
top level: BBa_B1010
start: 32
end: 34
orientation: inline

B1010
type: SequenceAnnotation
persistent identity: annotation1901013
display id: annotation1901013
version: 1
top level: BBa_B1010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901013/range1901013/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901013/range1901013/1
type: Range
persistent identity: range1901013
display id: range1901013
version: 1
direction: direction/forward
top level: BBa_B1010
start: 1
end: 40
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1901014
display id: annotation1901014
version: 1
top level: BBa_B1010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901014/range1901014/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901014/range1901014/1
type: Range
persistent identity: range1901014
display id: range1901014
version: 1
direction: direction/forward
top level: BBa_B1010
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901015
display id: annotation1901015
version: 1
top level: BBa_B1010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901015/range1901015/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901015/range1901015/1
type: Range
persistent identity: range1901015
display id: range1901015
version: 1
direction: direction/reverse
top level: BBa_B1010
start: 7
end: 9
orientation: inline

T22
type: ComponentDefinition
persistent identity: BBa K864501
display id: BBa_K864501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T22, P22 late terminator
creator: Erik Gullberg
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 10137
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Strong transcriptional terminator consisting of a 20 bp stem-loop from Salmonella phage P22.
mutable notes: Design based on McDowell et al 1994.
mutable provenance: Salmonella phage P22
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K864501_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2197326
display id: annotation2197326
version: 1
top level: T22
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864501/annotation2197326/range2197326/1

https://synbiohub.org/public/igem/BBa_K864501/annotation2197326/range2197326/1
type: Range
persistent identity: range2197326
display id: range2197326
version: 1
direction: direction/forward
top level: T22
start: 5
end: 34
orientation: inline