//viral_vectors/aav/miscellaneous
type: Collection
persistent identity: viral vectors aav miscellaneous
display id: viral_vectors_aav_miscellaneous
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K404212
member: BBa_K404105
member: BBa_K404303
member: BBa_K404090
member: BBa_K404209
member: BBa_K404205
member: BBa_K404304
member: BBa_K404211
member: BBa_K404214
member: BBa_K404103
member: BBa_K404202
member: BBa_K404094
member: BBa_K404107
member: BBa_K404305
member: BBa_K404092
member: BBa_K404102
member: BBa_K404201
member: BBa_K404207
member: BBa_K404306
member: BBa_K404093
member: BBa_K404091
member: BBa_K404104
member: BBa_K404203
member: BBa_K404302
member: BBa_K404213
member: BBa_K404208
member: BBa_K404307

//viral_vectors/aav/vector_plasmid
type: Collection
persistent identity: viral vectors aav vector plasmid
display id: viral_vectors_aav_vector_plasmid
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K404118
member: BBa_K404129
member: BBa_K404100
member: BBa_K404116
member: BBa_K404108
member: BBa_K404127
member: BBa_K404121
member: BBa_K404123
member: BBa_K404125
member: BBa_K404120
member: BBa_K404101
member: BBa_K404114
member: BBa_K404119
member: BBa_K404117
member: BBa_K404115
member: BBa_K404122
member: BBa_K404128
member: BBa_K404126
member: BBa_K404124

//viral_vectors/aav
type: Collection
persistent identity: viral vectors aav
display id: viral_vectors_aav
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K404250
member: BBa_K404006
member: BBa_K404164
member: BBa_K404241
member: BBa_K404155
member: BBa_K404209
member: BBa_K404125
member: BBa_K404248
member: BBa_K404211
member: BBa_K404232
member: BBa_K404202
member: BBa_K404253
member: BBa_K404227
member: BBa_K404206
member: BBa_K404167
member: BBa_K404223
member: BBa_K404158
member: BBa_K404108
member: BBa_K404011
member: BBa_K404235
member: BBa_K404101
member: BBa_K404203
member: BBa_K404160
member: BBa_K404161
member: BBa_K404126
member: BBa_K404151
member: BBa_K404121
member: BBa_K404254
member: BBa_K404168
member: BBa_K404224
member: BBa_K404205
member: BBa_K404119
member: BBa_K404128
member: //viral_vectors/aav/vector_plasmid
member: BBa_K404012
member: BBa_K404003
member: BBa_K404207
member: BBa_K404166
member: BBa_K404153
member: BBa_K404123
member: BBa_K404243
member: BBa_K404256
member: BBa_K404104
member: BBa_K404213
member: BBa_K404117
member: BBa_K404118
member: BBa_K404255
member: BBa_K404204
member: BBa_K404001
member: BBa_K404246
member: BBa_K404127
member: BBa_K404120
member: BBa_K404013
member: BBa_K404115
member: BBa_K404004
member: BBa_K404094
member: BBa_K404008
member: BBa_K404150
member: BBa_K404162
member: BBa_K404252
member: BBa_K404222
member: BBa_K404229
member: BBa_K404157
member: BBa_K404005
member: BBa_K404114
member: //viral_vectors/aav/miscellaneous
member: BBa_K404010
member: BBa_K404234
member: //viral_vectors/aav/capsid_coding
member: BBa_K404251
member: BBa_K404156
member: BBa_K404212

//viral_vectors
type: Collection
persistent identity: viral vectors
display id: viral_vectors
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K404250
member: BBa_K404255
member: BBa_K404006
member: BBa_K404164
member: BBa_K404204
member: BBa_K404001
member: BBa_K404246
member: BBa_K404241
member: BBa_K404127
member: BBa_K404155
member: BBa_K404209
member: BBa_K404248
member: BBa_K404013
member: BBa_K404211
member: BBa_K404232
member: BBa_K404115
member: BBa_K404004
member: BBa_K404202
member: BBa_K404253
member: BBa_K404094
member: BBa_K404227
member: BBa_K404008
member: BBa_K404150
member: BBa_K404206
member: BBa_K404223
member: BBa_K404158
member: BBa_K404162
member: BBa_K404011
member: BBa_K404235
member: BBa_K404252
member: BBa_K404222
member: BBa_K404229
member: BBa_K404157
member: BBa_K404005
member: BBa_K404101
member: BBa_K404203
member: BBa_K404114
member: BBa_K404160
member: BBa_K404010
member: BBa_K404161
member: BBa_K404234
member: BBa_K404251
member: BBa_K404151
member: BBa_K404156
member: BBa_K404212
member: BBa_K404105
member: BBa_K404242
member: BBa_K404247
member: BBa_K404090
member: BBa_K404100
member: BBa_K404254
member: BBa_K404168
member: BBa_K404224
member: BBa_K404007
member: BBa_K404205
member: BBa_K404163
member: //viral_vectors/aav
member: BBa_K404214
member: BBa_K404103
member: BBa_K404154
member: BBa_K404128
member: BBa_K404012
member: BBa_K404210
member: BBa_K404236
member: BBa_K404129
member: BBa_K404092
member: BBa_K404003
member: BBa_K404201
member: BBa_K404244
member: BBa_K404009
member: BBa_K404207
member: BBa_K404159

//viral_vectors/aav/capsid_coding
type: Collection
persistent identity: viral vectors aav capsid coding
display id: viral_vectors_aav_capsid_coding
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K404250
member: BBa_K404255
member: BBa_K404006
member: BBa_K404164
member: BBa_K404204
member: BBa_K404001
member: BBa_K404246
member: BBa_K404241
member: BBa_K404155
member: BBa_K404248
member: BBa_K404013
member: BBa_K404232
member: BBa_K404004
member: BBa_K404253
member: BBa_K404227
member: BBa_K404008
member: BBa_K404150
member: BBa_K404206
member: BBa_K404167
member: BBa_K404223
member: BBa_K404158
member: BBa_K404162
member: BBa_K404228
member: BBa_K404215
member: BBa_K404011
member: BBa_K404235
member: BBa_K404252
member: BBa_K404222
member: BBa_K404229
member: BBa_K404157
member: BBa_K404005
member: BBa_K404160
member: BBa_K404216
member: BBa_K404221
member: BBa_K404010
member: BBa_K404161
member: BBa_K404234
member: BBa_K404251
member: BBa_K404151
member: BBa_K404156
member: BBa_K404242
member: BBa_K404247
member: BBa_K404226
member: BBa_K404233
member: BBa_K404254
member: BBa_K404168
member: BBa_K404224
member: BBa_K404007
member: BBa_K404163
member: BBa_K404154
member: BBa_K404012
member: BBa_K404210
member: BBa_K404231
member: BBa_K404236
member: BBa_K404003
member: BBa_K404244
member: BBa_K404314
member: BBa_K404009
member: BBa_K404159
member: BBa_K404166
member: BBa_K404153
member: BBa_K404243
member: BBa_K404256
member: BBa_K404002
member: BBa_K404230
member: BBa_K404165
member: BBa_K404225
member: BBa_K404152

BBa_K404129
type: ComponentDefinition
persistent identity: BBa K404129
display id: BBa_K404129
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_phTERT_betaglobin_mCherry_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: right ITR
sequence annotation: Beta-Globin-intron
sequence annotation: hGH terminator
sequence annotation: rrnB _T1
sequence annotation: phTERT
sequence annotation: mCherry
sequence annotation: left ITR
sequence annotation: stem loop early T7
sequence: https://synbiohub.org/public/igem/BBa_K404129_sequence/1

right ITR
type: SequenceAnnotation
persistent identity: annotation2093320
display id: annotation2093320
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093320/range2093320/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093320/range2093320/1
type: Range
persistent identity: range2093320
display id: range2093320
version: 1
direction: direction/forward
top level: BBa_K404129
start: 2491
end: 2629
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093318
display id: annotation2093318
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093318/range2093318/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093318/range2093318/1
type: Range
persistent identity: range2093318
display id: range2093318
version: 1
direction: direction/forward
top level: BBa_K404129
start: 619
end: 1113
orientation: inline

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2093321
display id: annotation2093321
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093321/range2093321/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093321/range2093321/1
type: Range
persistent identity: range2093321
display id: range2093321
version: 1
direction: direction/forward
top level: BBa_K404129
start: 1998
end: 2476
orientation: inline

rrnB _T1
type: SequenceAnnotation
persistent identity: annotation2093335
display id: annotation2093335
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093335/range2093335/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093335/range2093335/1
type: Range
persistent identity: range2093335
display id: range2093335
version: 1
direction: direction/forward
top level: BBa_K404129
start: 1872
end: 1916
orientation: inline

phTERT
type: SequenceAnnotation
persistent identity: annotation2093316
display id: annotation2093316
version: 1
top level: BBa_K404129
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093316/range2093316/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093316/range2093316/1
type: Range
persistent identity: range2093316
display id: range2093316
version: 1
direction: direction/forward
top level: BBa_K404129
start: 154
end: 612
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2093319
display id: annotation2093319
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093319/range2093319/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093319/range2093319/1
type: Range
persistent identity: range2093319
display id: range2093319
version: 1
direction: direction/forward
top level: BBa_K404129
start: 1139
end: 1849
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093315
display id: annotation2093315
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093315/range2093315/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093315/range2093315/1
type: Range
persistent identity: range2093315
display id: range2093315
version: 1
direction: direction/forward
top level: BBa_K404129
start: 1
end: 147
orientation: inline

stem loop early T7
type: SequenceAnnotation
persistent identity: annotation2093336
display id: annotation2093336
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093336/range2093336/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093336/range2093336/1
type: Range
persistent identity: range2093336
display id: range2093336
version: 1
direction: direction/forward
top level: BBa_K404129
start: 1956
end: 1975
orientation: inline

BBa_K404127
type: ComponentDefinition
persistent identity: BBa K404127
display id: BBa_K404127
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_pCMV_betaglobin_mVenus_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV promoter
sequence annotation: Beta-Globin-intron
sequence annotation: right ITR
sequence annotation: left ITR
sequence annotation: mVenus
sequence: https://synbiohub.org/public/igem/BBa_K404127_sequence/1

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093299
display id: annotation2093299
version: 1
top level: BBa_K404127
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404127/annotation2093299/range2093299/1

https://synbiohub.org/public/igem/BBa_K404127/annotation2093299/range2093299/1
type: Range
persistent identity: range2093299
display id: range2093299
version: 1
direction: direction/forward
top level: BBa_K404127
start: 155
end: 809
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093300
display id: annotation2093300
version: 1
top level: BBa_K404127
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404127/annotation2093300/range2093300/1

https://synbiohub.org/public/igem/BBa_K404127/annotation2093300/range2093300/1
type: Range
persistent identity: range2093300
display id: range2093300
version: 1
direction: direction/forward
top level: BBa_K404127
start: 816
end: 1310
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093302
display id: annotation2093302
version: 1
top level: BBa_K404127
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404127/annotation2093302/range2093302/1

https://synbiohub.org/public/igem/BBa_K404127/annotation2093302/range2093302/1
type: Range
persistent identity: range2093302
display id: range2093302
version: 1
direction: direction/forward
top level: BBa_K404127
start: 2062
end: 2199
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093298
display id: annotation2093298
version: 1
top level: BBa_K404127
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404127/annotation2093298/range2093298/1

https://synbiohub.org/public/igem/BBa_K404127/annotation2093298/range2093298/1
type: Range
persistent identity: range2093298
display id: range2093298
version: 1
direction: direction/forward
top level: BBa_K404127
start: 1
end: 147
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2093301
display id: annotation2093301
version: 1
top level: BBa_K404127
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404127/annotation2093301/range2093301/1

https://synbiohub.org/public/igem/BBa_K404127/annotation2093301/range2093301/1
type: Range
persistent identity: range2093301
display id: range2093301
version: 1
direction: direction/forward
top level: BBa_K404127
start: 1325
end: 2038
orientation: inline

BBa_K404010
type: ComponentDefinition
persistent identity: BBa K404010
display id: BBa_K404010
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-VP2
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404010_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2093158
display id: annotation2093158
version: 1
top level: BBa_K404010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404010/annotation2093158/range2093158/1

https://synbiohub.org/public/igem/BBa_K404010/annotation2093158/range2093158/1
type: Range
persistent identity: range2093158
display id: range2093158
version: 1
direction: direction/forward
top level: BBa_K404010
start: 1
end: 1830
orientation: inline

BBa_K404104
type: ComponentDefinition
persistent identity: BBa K404104
display id: BBa_K404104
version: 1
was generated by: iGEM to SBOL conversion
description: p5-TATAless promoter
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: p5TATAless
sequence: https://synbiohub.org/public/igem/BBa_K404104_sequence/1

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2094942
display id: annotation2094942
version: 1
top level: BBa_K404104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404104/annotation2094942/range2094942/1

https://synbiohub.org/public/igem/BBa_K404104/annotation2094942/range2094942/1
type: Range
persistent identity: range2094942
display id: range2094942
version: 1
direction: direction/forward
top level: BBa_K404104
start: 1
end: 164
orientation: inline

BBa_K404122
type: ComponentDefinition
persistent identity: BBa K404122
display id: BBa_K404122
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_pCMV_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: scar
sequence annotation: RBE (Rep-binding element)
sequence annotation: CMV
sequence annotation: part of human beta-globin-exon III
sequence annotation: cleavage site
sequence annotation: beta-globin-intron II
sequence annotation: right ITR
sequence annotation: hGH
sequence annotation: part of chimeric CMV promoter
sequence annotation: left ITR
sequence annotation: intron 2 of human beta-globin
sequence annotation: Beta-Globin-intron
sequence annotation: DSE (downstream sequence element)
sequence annotation: polyA
sequence annotation: mGMK_TK30
sequence: https://synbiohub.org/public/igem/BBa_K404122_sequence/1

scar
type: SequenceAnnotation
persistent identity: annotation2101673
display id: annotation2101673
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101673/range2101673/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101673/range2101673/1
type: Range
persistent identity: range2101673
display id: range2101673
version: 1
direction: direction/forward
top level: BBa_K404122
start: 3020
end: 3027
orientation: inline

RBE (Rep-binding element)
type: SequenceAnnotation
persistent identity: annotation2101616
display id: annotation2101616
version: 1
top level: BBa_K404122
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101616/range2101616/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101616/range2101616/1
type: Range
persistent identity: range2101616
display id: range2101616
version: 1
direction: direction/forward
top level: BBa_K404122
start: 88
end: 103
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101674
display id: annotation2101674
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101674/range2101674/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101674/range2101674/1
type: Range
persistent identity: range2101674
display id: range2101674
version: 1
direction: direction/forward
top level: BBa_K404122
start: 3507
end: 3520
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101670
display id: annotation2101670
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101670/range2101670/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101670/range2101670/1
type: Range
persistent identity: range2101670
display id: range2101670
version: 1
direction: direction/forward
top level: BBa_K404122
start: 809
end: 816
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2101617
display id: annotation2101617
version: 1
top level: BBa_K404122
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101617/range2101617/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101617/range2101617/1
type: Range
persistent identity: range2101617
display id: range2101617
version: 1
direction: direction/forward
top level: BBa_K404122
start: 155
end: 808
orientation: inline

part of human beta-globin-exon III
type: SequenceAnnotation
persistent identity: annotation2101622
display id: annotation2101622
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101622/range2101622/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101622/range2101622/1
type: Range
persistent identity: range2101622
display id: range2101622
version: 1
direction: direction/forward
top level: BBa_K404122
start: 1252
end: 1304
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101668
display id: annotation2101668
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101668/range2101668/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101668/range2101668/1
type: Range
persistent identity: range2101668
display id: range2101668
version: 1
direction: direction/forward
top level: BBa_K404122
start: 141
end: 154
orientation: inline

cleavage site
type: SequenceAnnotation
persistent identity: annotation2101626
display id: annotation2101626
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101626/range2101626/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101626/range2101626/1
type: Range
persistent identity: range2101626
display id: range2101626
version: 1
direction: direction/reverse
top level: BBa_K404122
start: 3080
end: 3081
orientation: inline

beta-globin-intron II
type: SequenceAnnotation
persistent identity: annotation2101621
display id: annotation2101621
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101621/range2101621/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101621/range2101621/1
type: Range
persistent identity: range2101621
display id: range2101621
version: 1
direction: direction/forward
top level: BBa_K404122
start: 905
end: 1251
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2101628
display id: annotation2101628
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101628/range2101628/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101628/range2101628/1
type: Range
persistent identity: range2101628
display id: range2101628
version: 1
direction: direction/forward
top level: BBa_K404122
start: 3521
end: 3658
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2101624
display id: annotation2101624
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101624/range2101624/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101624/range2101624/1
type: Range
persistent identity: range2101624
display id: range2101624
version: 1
direction: direction/reverse
top level: BBa_K404122
start: 3028
end: 3506
orientation: inline

RBE (Rep-binding element)
type: SequenceAnnotation
persistent identity: annotation2101629
display id: annotation2101629
version: 1
top level: BBa_K404122
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101629/range2101629/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101629/range2101629/1
type: Range
persistent identity: range2101629
display id: range2101629
version: 1
direction: direction/forward
top level: BBa_K404122
start: 3632
end: 3647
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101672
display id: annotation2101672
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101672/range2101672/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101672/range2101672/1
type: Range
persistent identity: range2101672
display id: range2101672
version: 1
direction: direction/forward
top level: BBa_K404122
start: 1310
end: 1315
orientation: inline

part of chimeric CMV promoter
type: SequenceAnnotation
persistent identity: annotation2101619
display id: annotation2101619
version: 1
top level: BBa_K404122
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101619/range2101619/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101619/range2101619/1
type: Range
persistent identity: range2101619
display id: range2101619
version: 1
direction: direction/forward
top level: BBa_K404122
start: 824
end: 904
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2101615
display id: annotation2101615
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101615/range2101615/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101615/range2101615/1
type: Range
persistent identity: range2101615
display id: range2101615
version: 1
direction: direction/forward
top level: BBa_K404122
start: 6
end: 140
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101669
display id: annotation2101669
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101669/range2101669/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101669/range2101669/1
type: Range
persistent identity: range2101669
display id: range2101669
version: 1
direction: direction/forward
top level: BBa_K404122
start: 1
end: 5
orientation: inline

intron 2 of human beta-globin
type: SequenceAnnotation
persistent identity: annotation2101620
display id: annotation2101620
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101620/range2101620/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101620/range2101620/1
type: Range
persistent identity: range2101620
display id: range2101620
version: 1
direction: direction/forward
top level: BBa_K404122
start: 899
end: 1252
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2101618
display id: annotation2101618
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101618/range2101618/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101618/range2101618/1
type: Range
persistent identity: range2101618
display id: range2101618
version: 1
direction: direction/forward
top level: BBa_K404122
start: 817
end: 1309
orientation: inline

DSE (downstream sequence element)
type: SequenceAnnotation
persistent identity: annotation2101625
display id: annotation2101625
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101625/range2101625/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101625/range2101625/1
type: Range
persistent identity: range2101625
display id: range2101625
version: 1
direction: direction/reverse
top level: BBa_K404122
start: 3046
end: 3050
orientation: inline

polyA
type: SequenceAnnotation
persistent identity: annotation2101627
display id: annotation2101627
version: 1
top level: BBa_K404122
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101627/range2101627/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101627/range2101627/1
type: Range
persistent identity: range2101627
display id: range2101627
version: 1
direction: direction/forward
top level: BBa_K404122
start: 3110
end: 3115
orientation: inline

mGMK_TK30
type: SequenceAnnotation
persistent identity: annotation2101623
display id: annotation2101623
version: 1
top level: BBa_K404122
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101623/range2101623/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101623/range2101623/1
type: Range
persistent identity: range2101623
display id: range2101623
version: 1
direction: direction/forward
top level: BBa_K404122
start: 1316
end: 3019
orientation: inline

BBa_K404162
type: ComponentDefinition
persistent identity: BBa K404162
display id: BBa_K404162
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-BAP)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: downstream 587
sequence annotation: HSPG-KO
sequence annotation: CMV
sequence annotation: TG-spacer
sequence annotation: BAP
sequence annotation: upstream 587
sequence annotation: VP3
sequence annotation: GLS-spacer
sequence annotation: ZEGFR_1907
sequence annotation: middle-linker
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404162_sequence/1

downstream 587
type: SequenceAnnotation
persistent identity: annotation2095021
display id: annotation2095021
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095021/range2095021/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095021/range2095021/1
type: Range
persistent identity: range2095021
display id: range2095021
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2288
end: 2303
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101812
display id: annotation2101812
version: 1
top level: BBa_K404162
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2101812/range2101812/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2101812/range2101812/1
type: Range
persistent identity: range2101812
display id: range2101812
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2219
end: 2221
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095012
display id: annotation2095012
version: 1
top level: BBa_K404162
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095012/range2095012/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095012/range2095012/1
type: Range
persistent identity: range2095012
display id: range2095012
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2
end: 655
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2095019
display id: annotation2095019
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095019/range2095019/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095019/range2095019/1
type: Range
persistent identity: range2095019
display id: range2095019
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2228
end: 2233
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101813
display id: annotation2101813
version: 1
top level: BBa_K404162
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2101813/range2101813/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2101813/range2101813/1
type: Range
persistent identity: range2101813
display id: range2101813
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2288
end: 2290
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2095018
display id: annotation2095018
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095018/range2095018/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095018/range2095018/1
type: Range
persistent identity: range2095018
display id: range2095018
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2234
end: 2278
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2095022
display id: annotation2095022
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095022/range2095022/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095022/range2095022/1
type: Range
persistent identity: range2095022
display id: range2095022
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2196
end: 2227
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095016
display id: annotation2095016
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095016/range2095016/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095016/range2095016/1
type: Range
persistent identity: range2095016
display id: range2095016
version: 1
direction: direction/forward
top level: BBa_K404162
start: 1073
end: 2734
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2095020
display id: annotation2095020
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095020/range2095020/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095020/range2095020/1
type: Range
persistent identity: range2095020
display id: range2095020
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2279
end: 2287
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2095013
display id: annotation2095013
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095013/range2095013/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095013/range2095013/1
type: Range
persistent identity: range2095013
display id: range2095013
version: 1
direction: direction/forward
top level: BBa_K404162
start: 671
end: 844
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2095014
display id: annotation2095014
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095014/range2095014/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095014/range2095014/1
type: Range
persistent identity: range2095014
display id: range2095014
version: 1
direction: direction/forward
top level: BBa_K404162
start: 851
end: 874
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095017
display id: annotation2095017
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095017/range2095017/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095017/range2095017/1
type: Range
persistent identity: range2095017
display id: range2095017
version: 1
direction: direction/forward
top level: BBa_K404162
start: 881
end: 2857
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095015
display id: annotation2095015
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095015/range2095015/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095015/range2095015/1
type: Range
persistent identity: range2095015
display id: range2095015
version: 1
direction: direction/forward
top level: BBa_K404162
start: 881
end: 2734
orientation: inline

BBa_K404306
type: ComponentDefinition
persistent identity: BBa K404306
display id: BBa_K404306
version: 1
was generated by: iGEM to SBOL conversion
description: Z-EGFR-1907_SEG-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: SEG-linker
sequence annotation: Z-EGFR-1907
sequence: https://synbiohub.org/public/igem/BBa_K404306_sequence/1

SEG-linker
type: SequenceAnnotation
persistent identity: annotation2094758
display id: annotation2094758
version: 1
top level: BBa_K404306
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404306/annotation2094758/range2094758/1

https://synbiohub.org/public/igem/BBa_K404306/annotation2094758/range2094758/1
type: Range
persistent identity: range2094758
display id: range2094758
version: 1
direction: direction/forward
top level: BBa_K404306
start: 181
end: 288
orientation: inline

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094759
display id: annotation2094759
version: 1
top level: BBa_K404306
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404306/annotation2094759/range2094759/1

https://synbiohub.org/public/igem/BBa_K404306/annotation2094759/range2094759/1
type: Range
persistent identity: range2094759
display id: range2094759
version: 1
direction: direction/forward
top level: BBa_K404306
start: 1
end: 175
orientation: inline

BBa_K404154
type: ComponentDefinition
persistent identity: BBa K404154
display id: BBa_K404154
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_Z-EGFR-1907_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence annotation: HSPG-KO
sequence annotation: ZEGFR_1907
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404154_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2097912
display id: annotation2097912
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097912/range2097912/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097912/range2097912/1
type: Range
persistent identity: range2097912
display id: range2097912
version: 1
direction: direction/forward
top level: BBa_K404154
start: 1043
end: 2644
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097913
display id: annotation2097913
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097913/range2097913/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097913/range2097913/1
type: Range
persistent identity: range2097913
display id: range2097913
version: 1
direction: direction/forward
top level: BBa_K404154
start: 851
end: 2767
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097910
display id: annotation2097910
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097910/range2097910/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097910/range2097910/1
type: Range
persistent identity: range2097910
display id: range2097910
version: 1
direction: direction/forward
top level: BBa_K404154
start: 851
end: 2644
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101717
display id: annotation2101717
version: 1
top level: BBa_K404154
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2101717/range2101717/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2101717/range2101717/1
type: Range
persistent identity: range2101717
display id: range2101717
version: 1
direction: direction/forward
top level: BBa_K404154
start: 2198
end: 2200
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2097909
display id: annotation2097909
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097909/range2097909/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097909/range2097909/1
type: Range
persistent identity: range2097909
display id: range2097909
version: 1
direction: direction/forward
top level: BBa_K404154
start: 671
end: 844
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097908
display id: annotation2097908
version: 1
top level: BBa_K404154
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097908/range2097908/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097908/range2097908/1
type: Range
persistent identity: range2097908
display id: range2097908
version: 1
direction: direction/forward
top level: BBa_K404154
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101718
display id: annotation2101718
version: 1
top level: BBa_K404154
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2101718/range2101718/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2101718/range2101718/1
type: Range
persistent identity: range2101718
display id: range2101718
version: 1
direction: direction/forward
top level: BBa_K404154
start: 2189
end: 2191
orientation: inline

BBa_K404121
type: ComponentDefinition
persistent identity: BBa K404121
display id: BBa_K404121
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_pCMV_betaglobin_mCherry_hGH_[AAV2]-right-ITR
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
mutable provenance: x
owned by: james
owned by: myers
mutable description: x
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: x
star: false
creator: Freiburg Bioware 2010
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: mCherry
sequence annotation: right ITR
sequence annotation: hGH
sequence annotation: rrnB_T1
sequence annotation: left ITR
sequence annotation: Beta-Globin-intron
sequence annotation: CMV promoter
sequence annotation: stem loop early T7
sequence: https://synbiohub.org/public/igem/BBa_K404121_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation2093262
display id: annotation2093262
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093262/range2093262/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093262/range2093262/1
type: Range
persistent identity: range2093262
display id: range2093262
version: 1
top level: BBa_K404121
direction: direction/forward
start: 1336
end: 2046
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093265
display id: annotation2093265
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093265/range2093265/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093265/range2093265/1
type: Range
persistent identity: range2093265
display id: range2093265
version: 1
top level: BBa_K404121
direction: direction/forward
start: 2688
end: 2826
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2093266
display id: annotation2093266
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093266/range2093266/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093266/range2093266/1
type: Range
persistent identity: range2093266
display id: range2093266
version: 1
top level: BBa_K404121
direction: direction/forward
start: 2198
end: 2664
orientation: inline

rrnB_T1
type: SequenceAnnotation
persistent identity: annotation2093263
display id: annotation2093263
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093263/range2093263/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093263/range2093263/1
type: Range
persistent identity: range2093263
display id: range2093263
version: 1
top level: BBa_K404121
direction: direction/forward
start: 2069
end: 2112
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093259
display id: annotation2093259
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093259/range2093259/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093259/range2093259/1
type: Range
persistent identity: range2093259
display id: range2093259
version: 1
top level: BBa_K404121
direction: direction/forward
start: 1
end: 147
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093261
display id: annotation2093261
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093261/range2093261/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093261/range2093261/1
type: Range
persistent identity: range2093261
display id: range2093261
version: 1
top level: BBa_K404121
direction: direction/forward
start: 816
end: 1310
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093260
display id: annotation2093260
version: 1
top level: BBa_K404121
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093260/range2093260/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093260/range2093260/1
type: Range
persistent identity: range2093260
display id: range2093260
version: 1
top level: BBa_K404121
direction: direction/forward
start: 155
end: 809
orientation: inline

stem loop early T7
type: SequenceAnnotation
persistent identity: annotation2093264
display id: annotation2093264
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093264/range2093264/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093264/range2093264/1
type: Range
persistent identity: range2093264
display id: range2093264
version: 1
top level: BBa_K404121
direction: direction/forward
start: 2153
end: 2172
orientation: inline

BBa_K404124
type: ComponentDefinition
persistent identity: BBa K404124
display id: BBa_K404124
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_phTERT_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: y
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: phTERT
sequence annotation: Beta-Globin-intron
sequence annotation: right ITR
sequence annotation: hGH
sequence annotation: left ITR
sequence annotation: mGMK_TK30
sequence: https://synbiohub.org/public/igem/BBa_K404124_sequence/1

phTERT
type: SequenceAnnotation
persistent identity: annotation2095415
display id: annotation2095415
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095415/range2095415/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095415/range2095415/1
type: Range
persistent identity: range2095415
display id: range2095415
version: 1
direction: direction/forward
top level: BBa_K404124
start: 154
end: 612
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2095416
display id: annotation2095416
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095416/range2095416/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095416/range2095416/1
type: Range
persistent identity: range2095416
display id: range2095416
version: 1
direction: direction/forward
top level: BBa_K404124
start: 620
end: 1112
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2095578
display id: annotation2095578
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095578/range2095578/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095578/range2095578/1
type: Range
persistent identity: range2095578
display id: range2095578
version: 1
direction: direction/forward
top level: BBa_K404124
start: 3324
end: 3461
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2095520
display id: annotation2095520
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095520/range2095520/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095520/range2095520/1
type: Range
persistent identity: range2095520
display id: range2095520
version: 1
direction: direction/reverse
top level: BBa_K404124
start: 2831
end: 3309
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2095364
display id: annotation2095364
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095364/range2095364/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095364/range2095364/1
type: Range
persistent identity: range2095364
display id: range2095364
version: 1
direction: direction/forward
top level: BBa_K404124
start: 1
end: 147
orientation: inline

mGMK_TK30
type: SequenceAnnotation
persistent identity: annotation2095518
display id: annotation2095518
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095518/range2095518/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095518/range2095518/1
type: Range
persistent identity: range2095518
display id: range2095518
version: 1
direction: direction/forward
top level: BBa_K404124
start: 1119
end: 2822
orientation: inline

BBa_K404209
type: ComponentDefinition
persistent identity: BBa K404209
display id: BBa_K404209
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-587KO-BAP
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BAP-motif
sequence annotation: R=>A
sequence annotation: upstream 453
sequence annotation: downstream 453
sequence annotation: TG Spacer
sequence annotation: upstream 587
sequence annotation: GLS Spacer
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404209_sequence/1

BAP-motif
type: SequenceAnnotation
persistent identity: annotation2079120
display id: annotation2079120
version: 1
top level: BBa_K404209
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404209/annotation2079120/range2079120/1

https://synbiohub.org/public/igem/BBa_K404209/annotation2079120/range2079120/1
type: Range
persistent identity: range2079120
display id: range2079120
version: 1
direction: direction/forward
top level: BBa_K404209
start: 49
end: 93
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079123
display id: annotation2079123
version: 1
top level: BBa_K404209
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404209/annotation2079123/range2079123/1

https://synbiohub.org/public/igem/BBa_K404209/annotation2079123/range2079123/1
type: Range
persistent identity: range2079123
display id: range2079123
version: 1
direction: direction/forward
top level: BBa_K404209
start: 103
end: 105
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079116
display id: annotation2079116
version: 1
top level: BBa_K404209
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404209/annotation2079116/range2079116/1

https://synbiohub.org/public/igem/BBa_K404209/annotation2079116/range2079116/1
type: Range
persistent identity: range2079116
display id: range2079116
version: 1
direction: direction/forward
top level: BBa_K404209
start: 1
end: 9
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079124
display id: annotation2079124
version: 1
top level: BBa_K404209
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404209/annotation2079124/range2079124/1

https://synbiohub.org/public/igem/BBa_K404209/annotation2079124/range2079124/1
type: Range
persistent identity: range2079124
display id: range2079124
version: 1
direction: direction/forward
top level: BBa_K404209
start: 124
end: 132
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2079119
display id: annotation2079119
version: 1
top level: BBa_K404209
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404209/annotation2079119/range2079119/1

https://synbiohub.org/public/igem/BBa_K404209/annotation2079119/range2079119/1
type: Range
persistent identity: range2079119
display id: range2079119
version: 1
direction: direction/forward
top level: BBa_K404209
start: 43
end: 48
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079117
display id: annotation2079117
version: 1
top level: BBa_K404209
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404209/annotation2079117/range2079117/1

https://synbiohub.org/public/igem/BBa_K404209/annotation2079117/range2079117/1
type: Range
persistent identity: range2079117
display id: range2079117
version: 1
direction: direction/forward
top level: BBa_K404209
start: 10
end: 42
orientation: inline

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2079121
display id: annotation2079121
version: 1
top level: BBa_K404209
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404209/annotation2079121/range2079121/1

https://synbiohub.org/public/igem/BBa_K404209/annotation2079121/range2079121/1
type: Range
persistent identity: range2079121
display id: range2079121
version: 1
direction: direction/forward
top level: BBa_K404209
start: 94
end: 102
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079118
display id: annotation2079118
version: 1
top level: BBa_K404209
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404209/annotation2079118/range2079118/1

https://synbiohub.org/public/igem/BBa_K404209/annotation2079118/range2079118/1
type: Range
persistent identity: range2079118
display id: range2079118
version: 1
direction: direction/forward
top level: BBa_K404209
start: 34
end: 36
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079122
display id: annotation2079122
version: 1
top level: BBa_K404209
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404209/annotation2079122/range2079122/1

https://synbiohub.org/public/igem/BBa_K404209/annotation2079122/range2079122/1
type: Range
persistent identity: range2079122
display id: range2079122
version: 1
direction: direction/forward
top level: BBa_K404209
start: 103
end: 123
orientation: inline

BBa_K404208
type: ComponentDefinition
persistent identity: BBa K404208
display id: BBa_K404208
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-587-Beta Lactamase (BLA)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: downstream 587
sequence annotation: upstream 453
sequence annotation: Z34C motif
sequence annotation: upstream 587
sequence annotation: downstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404208_sequence/1

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079114
display id: annotation2079114
version: 1
top level: BBa_K404208
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404208/annotation2079114/range2079114/1

https://synbiohub.org/public/igem/BBa_K404208/annotation2079114/range2079114/1
type: Range
persistent identity: range2079114
display id: range2079114
version: 1
direction: direction/forward
top level: BBa_K404208
start: 145
end: 165
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079111
display id: annotation2079111
version: 1
top level: BBa_K404208
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404208/annotation2079111/range2079111/1

https://synbiohub.org/public/igem/BBa_K404208/annotation2079111/range2079111/1
type: Range
persistent identity: range2079111
display id: range2079111
version: 1
direction: direction/forward
top level: BBa_K404208
start: 1
end: 9
orientation: inline

Z34C motif
type: SequenceAnnotation
persistent identity: annotation2079113
display id: annotation2079113
version: 1
top level: BBa_K404208
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404208/annotation2079113/range2079113/1

https://synbiohub.org/public/igem/BBa_K404208/annotation2079113/range2079113/1
type: Range
persistent identity: range2079113
display id: range2079113
version: 1
direction: direction/forward
top level: BBa_K404208
start: 43
end: 144
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079112
display id: annotation2079112
version: 1
top level: BBa_K404208
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404208/annotation2079112/range2079112/1

https://synbiohub.org/public/igem/BBa_K404208/annotation2079112/range2079112/1
type: Range
persistent identity: range2079112
display id: range2079112
version: 1
direction: direction/forward
top level: BBa_K404208
start: 10
end: 42
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079115
display id: annotation2079115
version: 1
top level: BBa_K404208
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404208/annotation2079115/range2079115/1

https://synbiohub.org/public/igem/BBa_K404208/annotation2079115/range2079115/1
type: Range
persistent identity: range2079115
display id: range2079115
version: 1
direction: direction/forward
top level: BBa_K404208
start: 166
end: 174
orientation: inline

BBa_K404221
type: ComponentDefinition
persistent identity: BBa K404221
display id: BBa_K404221
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-453-BAP)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: rep78
sequence annotation: rep40
sequence annotation: rep68
sequence annotation: rep52
sequence: https://synbiohub.org/public/igem/BBa_K404221_sequence/1

rep78
type: SequenceAnnotation
persistent identity: annotation2095283
display id: annotation2095283
version: 1
top level: BBa_K404221
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K404221/annotation2095283/range2095283/1

https://synbiohub.org/public/igem/BBa_K404221/annotation2095283/range2095283/1
type: Range
persistent identity: range2095283
display id: range2095283
version: 1
direction: direction/forward
top level: BBa_K404221
start: 6
end: 1865
orientation: inline

rep40
type: SequenceAnnotation
persistent identity: annotation2095289
display id: annotation2095289
version: 1
top level: BBa_K404221
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K404221/annotation2095289/range2095289/1

https://synbiohub.org/public/igem/BBa_K404221/annotation2095289/range2095289/1
type: Range
persistent identity: range2095289
display id: range2095289
version: 1
direction: direction/forward
top level: BBa_K404221
start: 672
end: 1931
orientation: inline

rep68
type: SequenceAnnotation
persistent identity: annotation2095280
display id: annotation2095280
version: 1
top level: BBa_K404221
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K404221/annotation2095280/range2095280/1

https://synbiohub.org/public/igem/BBa_K404221/annotation2095280/range2095280/1
type: Range
persistent identity: range2095280
display id: range2095280
version: 1
direction: direction/forward
top level: BBa_K404221
start: 6
end: 1931
orientation: inline

rep52
type: SequenceAnnotation
persistent identity: annotation2095291
display id: annotation2095291
version: 1
top level: BBa_K404221
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K404221/annotation2095291/range2095291/1

https://synbiohub.org/public/igem/BBa_K404221/annotation2095291/range2095291/1
type: Range
persistent identity: range2095291
display id: range2095291
version: 1
direction: direction/forward
top level: BBa_K404221
start: 672
end: 1865
orientation: inline

BBa_K404006
type: ComponentDefinition
persistent identity: BBa K404006
display id: BBa_K404006
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-VP123
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404006_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2092161
display id: annotation2092161
version: 1
top level: BBa_K404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404006/annotation2092161/range2092161/1

https://synbiohub.org/public/igem/BBa_K404006/annotation2092161/range2092161/1
type: Range
persistent identity: range2092161
display id: range2092161
version: 1
direction: direction/forward
top level: BBa_K404006
start: 412
end: 2208
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2092162
display id: annotation2092162
version: 1
top level: BBa_K404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404006/annotation2092162/range2092162/1

https://synbiohub.org/public/igem/BBa_K404006/annotation2092162/range2092162/1
type: Range
persistent identity: range2092162
display id: range2092162
version: 1
direction: direction/forward
top level: BBa_K404006
start: 631
end: 2208
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2092160
display id: annotation2092160
version: 1
top level: BBa_K404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404006/annotation2092160/range2092160/1

https://synbiohub.org/public/igem/BBa_K404006/annotation2092160/range2092160/1
type: Range
persistent identity: range2092160
display id: range2092160
version: 1
direction: direction/forward
top level: BBa_K404006
start: 1
end: 2208
orientation: inline

BBa_K404314
type: ComponentDefinition
persistent identity: BBa K404314
display id: BBa_K404314
version: 1
was generated by: iGEM to SBOL conversion
description: DARPin-E01
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: DARPin-E01
sequence: https://synbiohub.org/public/igem/BBa_K404314_sequence/1

DARPin-E01
type: SequenceAnnotation
persistent identity: annotation2094760
display id: annotation2094760
version: 1
top level: BBa_K404314
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404314/annotation2094760/range2094760/1

https://synbiohub.org/public/igem/BBa_K404314/annotation2094760/range2094760/1
type: Range
persistent identity: range2094760
display id: range2094760
version: 1
direction: direction/forward
top level: BBa_K404314
start: 1
end: 459
orientation: inline

BBa_K404203
type: ComponentDefinition
persistent identity: BBa K404203
display id: BBa_K404203
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-453-RGD
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: downstream 453
sequence annotation: A Spacer
sequence annotation: RGD
sequence annotation: upstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404203_sequence/1

downstream 453
type: SequenceAnnotation
persistent identity: annotation2078943
display id: annotation2078943
version: 1
top level: BBa_K404203
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404203/annotation2078943/range2078943/1

https://synbiohub.org/public/igem/BBa_K404203/annotation2078943/range2078943/1
type: Range
persistent identity: range2078943
display id: range2078943
version: 1
direction: direction/forward
top level: BBa_K404203
start: 76
end: 96
orientation: inline

A Spacer
type: SequenceAnnotation
persistent identity: annotation2078940
display id: annotation2078940
version: 1
top level: BBa_K404203
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404203/annotation2078940/range2078940/1

https://synbiohub.org/public/igem/BBa_K404203/annotation2078940/range2078940/1
type: Range
persistent identity: range2078940
display id: range2078940
version: 1
direction: direction/forward
top level: BBa_K404203
start: 43
end: 45
orientation: inline

A Spacer
type: SequenceAnnotation
persistent identity: annotation2078942
display id: annotation2078942
version: 1
top level: BBa_K404203
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404203/annotation2078942/range2078942/1

https://synbiohub.org/public/igem/BBa_K404203/annotation2078942/range2078942/1
type: Range
persistent identity: range2078942
display id: range2078942
version: 1
direction: direction/forward
top level: BBa_K404203
start: 73
end: 75
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2078941
display id: annotation2078941
version: 1
top level: BBa_K404203
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404203/annotation2078941/range2078941/1

https://synbiohub.org/public/igem/BBa_K404203/annotation2078941/range2078941/1
type: Range
persistent identity: range2078941
display id: range2078941
version: 1
direction: direction/forward
top level: BBa_K404203
start: 46
end: 72
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2078939
display id: annotation2078939
version: 1
top level: BBa_K404203
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404203/annotation2078939/range2078939/1

https://synbiohub.org/public/igem/BBa_K404203/annotation2078939/range2078939/1
type: Range
persistent identity: range2078939
display id: range2078939
version: 1
direction: direction/forward
top level: BBa_K404203
start: 1
end: 42
orientation: inline

BBa_K404213
type: ComponentDefinition
persistent identity: BBa K404213
display id: BBa_K404213
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-587KO-Z34C
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: downstream 453
sequence annotation: upstream 453
sequence annotation: upstream 587
sequence annotation: R=>A
sequence annotation: downstream 587
sequence annotation: Z34C-motif
sequence: https://synbiohub.org/public/igem/BBa_K404213_sequence/1

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079163
display id: annotation2079163
version: 1
top level: BBa_K404213
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079163/range2079163/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079163/range2079163/1
type: Range
persistent identity: range2079163
display id: range2079163
version: 1
direction: direction/forward
top level: BBa_K404213
start: 166
end: 174
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079157
display id: annotation2079157
version: 1
top level: BBa_K404213
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079157/range2079157/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079157/range2079157/1
type: Range
persistent identity: range2079157
display id: range2079157
version: 1
direction: direction/forward
top level: BBa_K404213
start: 1
end: 9
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079158
display id: annotation2079158
version: 1
top level: BBa_K404213
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079158/range2079158/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079158/range2079158/1
type: Range
persistent identity: range2079158
display id: range2079158
version: 1
direction: direction/forward
top level: BBa_K404213
start: 10
end: 42
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079161
display id: annotation2079161
version: 1
top level: BBa_K404213
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079161/range2079161/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079161/range2079161/1
type: Range
persistent identity: range2079161
display id: range2079161
version: 1
direction: direction/forward
top level: BBa_K404213
start: 145
end: 147
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079162
display id: annotation2079162
version: 1
top level: BBa_K404213
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079162/range2079162/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079162/range2079162/1
type: Range
persistent identity: range2079162
display id: range2079162
version: 1
direction: direction/forward
top level: BBa_K404213
start: 145
end: 165
orientation: inline

Z34C-motif
type: SequenceAnnotation
persistent identity: annotation2079160
display id: annotation2079160
version: 1
top level: BBa_K404213
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079160/range2079160/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079160/range2079160/1
type: Range
persistent identity: range2079160
display id: range2079160
version: 1
direction: direction/forward
top level: BBa_K404213
start: 43
end: 144
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079159
display id: annotation2079159
version: 1
top level: BBa_K404213
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079159/range2079159/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079159/range2079159/1
type: Range
persistent identity: range2079159
display id: range2079159
version: 1
direction: direction/forward
top level: BBa_K404213
start: 34
end: 36
orientation: inline

BBa_K404128
type: ComponentDefinition
persistent identity: BBa K404128
display id: BBa_K404128
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_pCMV_mVenus_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: hGH
sequence annotation: CMV promter
sequence annotation: left ITR
sequence annotation: mVenus
sequence annotation: right ITR
sequence: https://synbiohub.org/public/igem/BBa_K404128_sequence/1

hGH
type: SequenceAnnotation
persistent identity: annotation2093314
display id: annotation2093314
version: 1
top level: BBa_K404128
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404128/annotation2093314/range2093314/1

https://synbiohub.org/public/igem/BBa_K404128/annotation2093314/range2093314/1
type: Range
persistent identity: range2093314
display id: range2093314
version: 1
direction: direction/forward
top level: BBa_K404128
start: 1558
end: 2037
orientation: inline

CMV promter
type: SequenceAnnotation
persistent identity: annotation2093306
display id: annotation2093306
version: 1
top level: BBa_K404128
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404128/annotation2093306/range2093306/1

https://synbiohub.org/public/igem/BBa_K404128/annotation2093306/range2093306/1
type: Range
persistent identity: range2093306
display id: range2093306
version: 1
direction: direction/forward
top level: BBa_K404128
start: 155
end: 809
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093305
display id: annotation2093305
version: 1
top level: BBa_K404128
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404128/annotation2093305/range2093305/1

https://synbiohub.org/public/igem/BBa_K404128/annotation2093305/range2093305/1
type: Range
persistent identity: range2093305
display id: range2093305
version: 1
direction: direction/forward
top level: BBa_K404128
start: 1
end: 147
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2093312
display id: annotation2093312
version: 1
top level: BBa_K404128
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404128/annotation2093312/range2093312/1

https://synbiohub.org/public/igem/BBa_K404128/annotation2093312/range2093312/1
type: Range
persistent identity: range2093312
display id: range2093312
version: 1
direction: direction/forward
top level: BBa_K404128
start: 828
end: 1541
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093313
display id: annotation2093313
version: 1
top level: BBa_K404128
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404128/annotation2093313/range2093313/1

https://synbiohub.org/public/igem/BBa_K404128/annotation2093313/range2093313/1
type: Range
persistent identity: range2093313
display id: range2093313
version: 1
direction: direction/forward
top level: BBa_K404128
start: 2051
end: 2189
orientation: inline

BBa_K404242
type: ComponentDefinition
persistent identity: BBa K404242
display id: BBa_K404242
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]VP123 (ViralBrick-453-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-09-19T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: downstream 453
sequence annotation: AAP
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: upstrem 453
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: His Tag
sequence annotation: VP2
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404242_sequence/1

downstream 453
type: SequenceAnnotation
persistent identity: annotation2094177
display id: annotation2094177
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094177/range2094177/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094177/range2094177/1
type: Range
persistent identity: range2094177
display id: range2094177
version: 1
direction: direction/forward
top level: BBa_K404242
start: 2051
end: 2064
orientation: inline

AAP
type: SequenceAnnotation
persistent identity: annotation2094205
display id: annotation2094205
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094205/range2094205/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094205/range2094205/1
type: Range
persistent identity: range2094205
display id: range2094205
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1194
end: 1808
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094174
display id: annotation2094174
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094174/range2094174/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094174/range2094174/1
type: Range
persistent identity: range2094174
display id: range2094174
version: 1
direction: direction/forward
top level: BBa_K404242
start: 671
end: 3022
orientation: inline

upstrem 453
type: SequenceAnnotation
persistent identity: annotation2094175
display id: annotation2094175
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094175/range2094175/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094175/range2094175/1
type: Range
persistent identity: range2094175
display id: range2094175
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1989
end: 2026
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094170
display id: annotation2094170
version: 1
top level: BBa_K404242
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094170/range2094170/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094170/range2094170/1
type: Range
persistent identity: range2094170
display id: range2094170
version: 1
direction: direction/forward
top level: BBa_K404242
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094173
display id: annotation2094173
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094173/range2094173/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094173/range2094173/1
type: Range
persistent identity: range2094173
display id: range2094173
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1274
end: 2899
orientation: inline

His Tag
type: SequenceAnnotation
persistent identity: annotation2094176
display id: annotation2094176
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094176/range2094176/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094176/range2094176/1
type: Range
persistent identity: range2094176
display id: range2094176
version: 1
direction: direction/forward
top level: BBa_K404242
start: 2030
end: 2047
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094172
display id: annotation2094172
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094172/range2094172/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094172/range2094172/1
type: Range
persistent identity: range2094172
display id: range2094172
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1079
end: 2899
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094171
display id: annotation2094171
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094171/range2094171/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094171/range2094171/1
type: Range
persistent identity: range2094171
display id: range2094171
version: 1
direction: direction/forward
top level: BBa_K404242
start: 671
end: 2899
orientation: inline

BBa_K404100
type: ComponentDefinition
persistent identity: BBa K404100
display id: BBa_K404100
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K404100_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2093166
display id: annotation2093166
version: 1
top level: BBa_K404100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404100/annotation2093166/range2093166/1

https://synbiohub.org/public/igem/BBa_K404100/annotation2093166/range2093166/1
type: Range
persistent identity: range2093166
display id: range2093166
version: 1
direction: direction/forward
top level: BBa_K404100
start: 1
end: 147
orientation: inline

BBa_K404302
type: ComponentDefinition
persistent identity: BBa K404302
display id: BBa_K404302
version: 1
was generated by: iGEM to SBOL conversion
description: Z-EGFR-1907
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Z-EGFR-1907
sequence: https://synbiohub.org/public/igem/BBa_K404302_sequence/1

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094740
display id: annotation2094740
version: 1
top level: BBa_K404302
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404302/annotation2094740/range2094740/1

https://synbiohub.org/public/igem/BBa_K404302/annotation2094740/range2094740/1
type: Range
persistent identity: range2094740
display id: range2094740
version: 1
direction: direction/forward
top level: BBa_K404302
start: 1
end: 174
orientation: inline

BBa_K404165
type: ComponentDefinition
persistent identity: BBa K404165
display id: BBa_K404165
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_VP1up_NLS_mVenus_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP 1
sequence annotation: VP 1 up
sequence annotation: HSPG-KO
sequence annotation: mVenus
sequence annotation: VP 2
sequence annotation: VP 3
sequence annotation: CMV
sequence annotation: AAP
sequence annotation: AAV-2 cap (Stratagene)
sequence annotation: NLS
sequence: https://synbiohub.org/public/igem/BBa_K404165_sequence/1

VP 1
type: SequenceAnnotation
persistent identity: annotation2099100
display id: annotation2099100
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099100/range2099100/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099100/range2099100/1
type: Range
persistent identity: range2099100
display id: range2099100
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1835
end: 3628
orientation: inline

VP 1 up
type: SequenceAnnotation
persistent identity: annotation2099095
display id: annotation2099095
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099095/range2099095/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099095/range2099095/1
type: Range
persistent identity: range2099095
display id: range2099095
version: 1
direction: direction/forward
top level: BBa_K404165
start: 673
end: 1081
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101816
display id: annotation2101816
version: 1
top level: BBa_K404165
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2101816/range2101816/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2101816/range2101816/1
type: Range
persistent identity: range2101816
display id: range2101816
version: 1
direction: direction/forward
top level: BBa_K404165
start: 3182
end: 3184
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2099097
display id: annotation2099097
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099097/range2099097/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099097/range2099097/1
type: Range
persistent identity: range2099097
display id: range2099097
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1115
end: 1828
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101817
display id: annotation2101817
version: 1
top level: BBa_K404165
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2101817/range2101817/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2101817/range2101817/1
type: Range
persistent identity: range2101817
display id: range2101817
version: 1
direction: direction/forward
top level: BBa_K404165
start: 3173
end: 3175
orientation: inline

VP 2
type: SequenceAnnotation
persistent identity: annotation2099098
display id: annotation2099098
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099098/range2099098/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099098/range2099098/1
type: Range
persistent identity: range2099098
display id: range2099098
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1835
end: 3628
orientation: inline

VP 3
type: SequenceAnnotation
persistent identity: annotation2099093
display id: annotation2099093
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099093/range2099093/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099093/range2099093/1
type: Range
persistent identity: range2099093
display id: range2099093
version: 1
direction: direction/forward
top level: BBa_K404165
start: 2027
end: 3628
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2099094
display id: annotation2099094
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099094/range2099094/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099094/range2099094/1
type: Range
persistent identity: range2099094
display id: range2099094
version: 1
direction: direction/forward
top level: BBa_K404165
start: 2
end: 655
orientation: inline

AAP
type: SequenceAnnotation
persistent identity: annotation2099101
display id: annotation2099101
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099101/range2099101/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099101/range2099101/1
type: Range
persistent identity: range2099101
display id: range2099101
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1947
end: 2561
orientation: inline

AAV-2 cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2099099
display id: annotation2099099
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099099/range2099099/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099099/range2099099/1
type: Range
persistent identity: range2099099
display id: range2099099
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1835
end: 3751
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2099096
display id: annotation2099096
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099096/range2099096/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099096/range2099096/1
type: Range
persistent identity: range2099096
display id: range2099096
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1088
end: 1108
orientation: inline

BBa_K404215
type: ComponentDefinition
persistent identity: BBa K404215
display id: BBa_K404215
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-453-Empty
creator: Freiburg Bioware 2010
created: 2010-10-12T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404091
type: ComponentDefinition
persistent identity: BBa K404091
display id: BBa_K404091
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep52ex
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rep52
sequence: https://synbiohub.org/public/igem/BBa_K404091_sequence/1

Rep52
type: SequenceAnnotation
persistent identity: annotation2093161
display id: annotation2093161
version: 1
top level: BBa_K404091
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404091/annotation2093161/range2093161/1

https://synbiohub.org/public/igem/BBa_K404091/annotation2093161/range2093161/1
type: Range
persistent identity: range2093161
display id: range2093161
version: 1
direction: direction/forward
top level: BBa_K404091
start: 1
end: 1195
orientation: inline

BBa_K404119
type: ComponentDefinition
persistent identity: BBa K404119
display id: BBa_K404119
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_pCMV_betaglobin_mVenus_hGH_[AAV2]-right-ITR
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
mutable provenance: x
owned by: james
owned by: myers
mutable description: x
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: x
star: false
creator: Freiburg Bioware 2010
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: left ITR
sequence annotation: Beta-Globin-intron
sequence annotation: right ITR
sequence annotation: mVenus
sequence annotation: CMV promoter
sequence annotation: hGH
sequence: https://synbiohub.org/public/igem/BBa_K404119_sequence/1

left ITR
type: SequenceAnnotation
persistent identity: annotation2093237
display id: annotation2093237
version: 1
top level: BBa_K404119
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093237/range2093237/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093237/range2093237/1
type: Range
persistent identity: range2093237
display id: range2093237
version: 1
top level: BBa_K404119
direction: direction/forward
start: 1
end: 147
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093239
display id: annotation2093239
version: 1
top level: BBa_K404119
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093239/range2093239/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093239/range2093239/1
type: Range
persistent identity: range2093239
display id: range2093239
version: 1
top level: BBa_K404119
direction: direction/forward
start: 816
end: 1310
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093242
display id: annotation2093242
version: 1
top level: BBa_K404119
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093242/range2093242/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093242/range2093242/1
type: Range
persistent identity: range2093242
display id: range2093242
version: 1
top level: BBa_K404119
direction: direction/forward
start: 2549
end: 2686
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2093241
display id: annotation2093241
version: 1
top level: BBa_K404119
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093241/range2093241/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093241/range2093241/1
type: Range
persistent identity: range2093241
display id: range2093241
version: 1
top level: BBa_K404119
direction: direction/forward
start: 1325
end: 2038
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093238
display id: annotation2093238
version: 1
top level: BBa_K404119
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093238/range2093238/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093238/range2093238/1
type: Range
persistent identity: range2093238
display id: range2093238
version: 1
top level: BBa_K404119
direction: direction/forward
start: 155
end: 808
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2093240
display id: annotation2093240
version: 1
top level: BBa_K404119
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093240/range2093240/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093240/range2093240/1
type: Range
persistent identity: range2093240
display id: range2093240
version: 1
top level: BBa_K404119
direction: direction/forward
start: 2056
end: 2535
orientation: inline

BBa_K404008
type: ComponentDefinition
persistent identity: BBa K404008
display id: BBa_K404008
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-VP1
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404008_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2107872
display id: annotation2107872
version: 1
top level: BBa_K404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404008/annotation2107872/range2107872/1

https://synbiohub.org/public/igem/BBa_K404008/annotation2107872/range2107872/1
type: Range
persistent identity: range2107872
display id: range2107872
version: 1
direction: direction/forward
top level: BBa_K404008
start: 1
end: 2202
orientation: inline

BBa_K404211
type: ComponentDefinition
persistent identity: BBa K404211
display id: BBa_K404211
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-587KO-His-Tag
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: upstream 453
sequence annotation: downstream 453
sequence annotation: GLS Spacer
sequence annotation: R=>A
sequence annotation: upstream 587
sequence annotation: His-Tag
sequence annotation: downstream 587
sequence annotation: TG Spacer
sequence: https://synbiohub.org/public/igem/BBa_K404211_sequence/1

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079131
display id: annotation2079131
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079131/range2079131/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079131/range2079131/1
type: Range
persistent identity: range2079131
display id: range2079131
version: 1
direction: direction/forward
top level: BBa_K404211
start: 1
end: 9
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079139
display id: annotation2079139
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079139/range2079139/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079139/range2079139/1
type: Range
persistent identity: range2079139
display id: range2079139
version: 1
direction: direction/forward
top level: BBa_K404211
start: 97
end: 105
orientation: inline

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2079136
display id: annotation2079136
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079136/range2079136/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079136/range2079136/1
type: Range
persistent identity: range2079136
display id: range2079136
version: 1
direction: direction/forward
top level: BBa_K404211
start: 67
end: 75
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079133
display id: annotation2079133
version: 1
top level: BBa_K404211
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079133/range2079133/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079133/range2079133/1
type: Range
persistent identity: range2079133
display id: range2079133
version: 1
direction: direction/forward
top level: BBa_K404211
start: 34
end: 36
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079132
display id: annotation2079132
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079132/range2079132/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079132/range2079132/1
type: Range
persistent identity: range2079132
display id: range2079132
version: 1
direction: direction/forward
top level: BBa_K404211
start: 10
end: 42
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079137
display id: annotation2079137
version: 1
top level: BBa_K404211
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079137/range2079137/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079137/range2079137/1
type: Range
persistent identity: range2079137
display id: range2079137
version: 1
direction: direction/forward
top level: BBa_K404211
start: 76
end: 78
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2079135
display id: annotation2079135
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079135/range2079135/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079135/range2079135/1
type: Range
persistent identity: range2079135
display id: range2079135
version: 1
direction: direction/forward
top level: BBa_K404211
start: 49
end: 66
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079138
display id: annotation2079138
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079138/range2079138/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079138/range2079138/1
type: Range
persistent identity: range2079138
display id: range2079138
version: 1
direction: direction/forward
top level: BBa_K404211
start: 76
end: 96
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2079134
display id: annotation2079134
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079134/range2079134/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079134/range2079134/1
type: Range
persistent identity: range2079134
display id: range2079134
version: 1
direction: direction/forward
top level: BBa_K404211
start: 43
end: 48
orientation: inline

BBa_K404159
type: ComponentDefinition
persistent identity: BBa K404159
display id: BBa_K404159
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_CFP_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: middle-linker
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: CFP
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404159_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2097914
display id: annotation2097914
version: 1
top level: BBa_K404159
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097914/range2097914/1

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type: Range
persistent identity: range2097914
display id: range2097914
version: 1
direction: direction/forward
top level: BBa_K404159
start: 2
end: 655
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2097916
display id: annotation2097916
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097916/range2097916/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097916/range2097916/1
type: Range
persistent identity: range2097916
display id: range2097916
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1391
end: 1414
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101798
display id: annotation2101798
version: 1
top level: BBa_K404159
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2101798/range2101798/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2101798/range2101798/1
type: Range
persistent identity: range2101798
display id: range2101798
version: 1
direction: direction/forward
top level: BBa_K404159
start: 2759
end: 2761
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097917
display id: annotation2097917
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097917/range2097917/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097917/range2097917/1
type: Range
persistent identity: range2097917
display id: range2097917
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1421
end: 3214
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101797
display id: annotation2101797
version: 1
top level: BBa_K404159
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2101797/range2101797/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2101797/range2101797/1
type: Range
persistent identity: range2101797
display id: range2101797
version: 1
direction: direction/forward
top level: BBa_K404159
start: 2768
end: 2770
orientation: inline

CFP
type: SequenceAnnotation
persistent identity: annotation2097915
display id: annotation2097915
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097915/range2097915/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097915/range2097915/1
type: Range
persistent identity: range2097915
display id: range2097915
version: 1
direction: direction/forward
top level: BBa_K404159
start: 662
end: 1385
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097919
display id: annotation2097919
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097919/range2097919/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097919/range2097919/1
type: Range
persistent identity: range2097919
display id: range2097919
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1421
end: 3337
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097918
display id: annotation2097918
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097918/range2097918/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097918/range2097918/1
type: Range
persistent identity: range2097918
display id: range2097918
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1613
end: 3214
orientation: inline

BBa_K404161
type: ComponentDefinition
persistent identity: BBa K404161
display id: BBa_K404161
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_DARPin-E01_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: middle-linker
sequence annotation: VP3
sequence annotation: HSPG-KO
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence annotation: DARPin-E01
sequence: https://synbiohub.org/public/igem/BBa_K404161_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2095006
display id: annotation2095006
version: 1
top level: BBa_K404161
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095006/range2095006/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095006/range2095006/1
type: Range
persistent identity: range2095006
display id: range2095006
version: 1
direction: direction/forward
top level: BBa_K404161
start: 2
end: 655
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2095008
display id: annotation2095008
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095008/range2095008/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095008/range2095008/1
type: Range
persistent identity: range2095008
display id: range2095008
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1136
end: 1159
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095010
display id: annotation2095010
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095010/range2095010/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095010/range2095010/1
type: Range
persistent identity: range2095010
display id: range2095010
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1358
end: 2959
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101801
display id: annotation2101801
version: 1
top level: BBa_K404161
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2101801/range2101801/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2101801/range2101801/1
type: Range
persistent identity: range2101801
display id: range2101801
version: 1
direction: direction/forward
top level: BBa_K404161
start: 2513
end: 2515
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095011
display id: annotation2095011
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095011/range2095011/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095011/range2095011/1
type: Range
persistent identity: range2095011
display id: range2095011
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1166
end: 3082
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101802
display id: annotation2101802
version: 1
top level: BBa_K404161
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2101802/range2101802/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2101802/range2101802/1
type: Range
persistent identity: range2101802
display id: range2101802
version: 1
direction: direction/forward
top level: BBa_K404161
start: 2504
end: 2506
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095009
display id: annotation2095009
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095009/range2095009/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095009/range2095009/1
type: Range
persistent identity: range2095009
display id: range2095009
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1166
end: 2959
orientation: inline

DARPin-E01
type: SequenceAnnotation
persistent identity: annotation2095007
display id: annotation2095007
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095007/range2095007/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095007/range2095007/1
type: Range
persistent identity: range2095007
display id: range2095007
version: 1
direction: direction/forward
top level: BBa_K404161
start: 666
end: 1130
orientation: inline

BBa_K404247
type: ComponentDefinition
persistent identity: BBa K404247
display id: BBa_K404247
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence annotation: downstream 587
sequence annotation: VP1
sequence annotation: upstream 587
sequence annotation: TG-spacer
sequence annotation: GLS-spacer
sequence annotation: His-tag
sequence annotation: CMV promoter
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404247_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094290
display id: annotation2094290
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094290/range2094290/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094290/range2094290/1
type: Range
persistent identity: range2094290
display id: range2094290
version: 1
direction: direction/forward
top level: BBa_K404247
start: 671
end: 3031
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094282
display id: annotation2094282
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094282/range2094282/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094282/range2094282/1
type: Range
persistent identity: range2094282
display id: range2094282
version: 1
direction: direction/forward
top level: BBa_K404247
start: 1079
end: 2908
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094287
display id: annotation2094287
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094287/range2094287/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094287/range2094287/1
type: Range
persistent identity: range2094287
display id: range2094287
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2462
end: 2477
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094281
display id: annotation2094281
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094281/range2094281/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094281/range2094281/1
type: Range
persistent identity: range2094281
display id: range2094281
version: 1
direction: direction/forward
top level: BBa_K404247
start: 671
end: 2908
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094288
display id: annotation2094288
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094288/range2094288/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094288/range2094288/1
type: Range
persistent identity: range2094288
display id: range2094288
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2397
end: 2428
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094285
display id: annotation2094285
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094285/range2094285/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094285/range2094285/1
type: Range
persistent identity: range2094285
display id: range2094285
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2429
end: 2434
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094286
display id: annotation2094286
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094286/range2094286/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094286/range2094286/1
type: Range
persistent identity: range2094286
display id: range2094286
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2453
end: 2461
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2094284
display id: annotation2094284
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094284/range2094284/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094284/range2094284/1
type: Range
persistent identity: range2094284
display id: range2094284
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2435
end: 2452
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094280
display id: annotation2094280
version: 1
top level: BBa_K404247
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094280/range2094280/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094280/range2094280/1
type: Range
persistent identity: range2094280
display id: range2094280
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094283
display id: annotation2094283
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094283/range2094283/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094283/range2094283/1
type: Range
persistent identity: range2094283
display id: range2094283
version: 1
direction: direction/forward
top level: BBa_K404247
start: 1274
end: 2908
orientation: inline

BBa_K404246
type: ComponentDefinition
persistent identity: BBa K404246
display id: BBa_K404246
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-BAP)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: TG-spacer
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence annotation: downstream 587
sequence annotation: upstream 587
sequence annotation: GLS-spacer
sequence annotation: VP1
sequence annotation: BAP
sequence annotation: CMV promoter
sequence: https://synbiohub.org/public/igem/BBa_K404246_sequence/1

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094275
display id: annotation2094275
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094275/range2094275/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094275/range2094275/1
type: Range
persistent identity: range2094275
display id: range2094275
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2429
end: 2434
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094271
display id: annotation2094271
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094271/range2094271/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094271/range2094271/1
type: Range
persistent identity: range2094271
display id: range2094271
version: 1
direction: direction/forward
top level: BBa_K404246
start: 1274
end: 2935
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094273
display id: annotation2094273
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094273/range2094273/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094273/range2094273/1
type: Range
persistent identity: range2094273
display id: range2094273
version: 1
direction: direction/forward
top level: BBa_K404246
start: 671
end: 3058
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094267
display id: annotation2094267
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094267/range2094267/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094267/range2094267/1
type: Range
persistent identity: range2094267
display id: range2094267
version: 1
direction: direction/forward
top level: BBa_K404246
start: 1079
end: 2935
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094277
display id: annotation2094277
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094277/range2094277/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094277/range2094277/1
type: Range
persistent identity: range2094277
display id: range2094277
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2489
end: 2504
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094278
display id: annotation2094278
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094278/range2094278/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094278/range2094278/1
type: Range
persistent identity: range2094278
display id: range2094278
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2397
end: 2428
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094276
display id: annotation2094276
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094276/range2094276/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094276/range2094276/1
type: Range
persistent identity: range2094276
display id: range2094276
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2480
end: 2488
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094266
display id: annotation2094266
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094266/range2094266/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094266/range2094266/1
type: Range
persistent identity: range2094266
display id: range2094266
version: 1
direction: direction/forward
top level: BBa_K404246
start: 671
end: 2935
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2094274
display id: annotation2094274
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094274/range2094274/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094274/range2094274/1
type: Range
persistent identity: range2094274
display id: range2094274
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2435
end: 2479
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094264
display id: annotation2094264
version: 1
top level: BBa_K404246
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094264/range2094264/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094264/range2094264/1
type: Range
persistent identity: range2094264
display id: range2094264
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2
end: 655
orientation: inline

BBa_K404225
type: ComponentDefinition
persistent identity: BBa K404225
display id: BBa_K404225
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587-BAP)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP 3
sequence annotation: PLA2
sequence annotation: Rep 68
sequence annotation: Rep78
sequence annotation: Rep 52
sequence annotation: VP 1
sequence annotation: TG-Spacer
sequence annotation: AAV-2 cap Stratagene
sequence annotation: Rep 40
sequence annotation: GLS-Spacer
sequence annotation: BAP
sequence annotation: VP 2
sequence annotation: p5 TATAless
sequence: https://synbiohub.org/public/igem/BBa_K404225_sequence/1

VP 3
type: SequenceAnnotation
persistent identity: annotation2099020
display id: annotation2099020
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099020/range2099020/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099020/range2099020/1
type: Range
persistent identity: range2099020
display id: range2099020
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2489
end: 4150
orientation: inline

PLA2
type: SequenceAnnotation
persistent identity: annotation2099005
display id: annotation2099005
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099005/range2099005/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099005/range2099005/1
type: Range
persistent identity: range2099005
display id: range2099005
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2015
end: 2191
orientation: inline

Rep 68
type: SequenceAnnotation
persistent identity: annotation2098997
display id: annotation2098997
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2098997/range2098997/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2098997/range2098997/1
type: Range
persistent identity: range2098997
display id: range2098997
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2
end: 1933
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098996
display id: annotation2098996
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2098996/range2098996/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2098996/range2098996/1
type: Range
persistent identity: range2098996
display id: range2098996
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2
end: 1867
orientation: inline

Rep 52
type: SequenceAnnotation
persistent identity: annotation2099003
display id: annotation2099003
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099003/range2099003/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099003/range2099003/1
type: Range
persistent identity: range2099003
display id: range2099003
version: 1
direction: direction/forward
top level: BBa_K404225
start: 673
end: 1867
orientation: inline

VP 1
type: SequenceAnnotation
persistent identity: annotation2099011
display id: annotation2099011
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099011/range2099011/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099011/range2099011/1
type: Range
persistent identity: range2099011
display id: range2099011
version: 1
direction: direction/forward
top level: BBa_K404225
start: 1883
end: 4150
orientation: inline

TG-Spacer
type: SequenceAnnotation
persistent identity: annotation2099026
display id: annotation2099026
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099026/range2099026/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099026/range2099026/1
type: Range
persistent identity: range2099026
display id: range2099026
version: 1
direction: direction/forward
top level: BBa_K404225
start: 3644
end: 3649
orientation: inline

AAV-2 cap Stratagene
type: SequenceAnnotation
persistent identity: annotation2099006
display id: annotation2099006
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099006/range2099006/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099006/range2099006/1
type: Range
persistent identity: range2099006
display id: range2099006
version: 1
direction: direction/forward
top level: BBa_K404225
start: 1883
end: 3753
orientation: inline

Rep 40
type: SequenceAnnotation
persistent identity: annotation2099004
display id: annotation2099004
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099004/range2099004/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099004/range2099004/1
type: Range
persistent identity: range2099004
display id: range2099004
version: 1
direction: direction/forward
top level: BBa_K404225
start: 673
end: 1933
orientation: inline

GLS-Spacer
type: SequenceAnnotation
persistent identity: annotation2099027
display id: annotation2099027
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099027/range2099027/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099027/range2099027/1
type: Range
persistent identity: range2099027
display id: range2099027
version: 1
direction: direction/forward
top level: BBa_K404225
start: 3695
end: 3703
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2099025
display id: annotation2099025
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099025/range2099025/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099025/range2099025/1
type: Range
persistent identity: range2099025
display id: range2099025
version: 1
direction: direction/forward
top level: BBa_K404225
start: 3650
end: 3694
orientation: inline

VP 2
type: SequenceAnnotation
persistent identity: annotation2099017
display id: annotation2099017
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099017/range2099017/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099017/range2099017/1
type: Range
persistent identity: range2099017
display id: range2099017
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2294
end: 4150
orientation: inline

p5 TATAless
type: SequenceAnnotation
persistent identity: annotation2099022
display id: annotation2099022
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099022/range2099022/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099022/range2099022/1
type: Range
persistent identity: range2099022
display id: range2099022
version: 1
direction: direction/forward
top level: BBa_K404225
start: 4282
end: 4445
orientation: inline

BBa_K404254
type: ComponentDefinition
persistent identity: BBa K404254
display id: BBa_K404254
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-RGD)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: upstream 587
sequence annotation: VP1
sequence annotation: GLS-spacer
sequence annotation: TG-spacer
sequence annotation: downstream 587
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: HSPG-KO
sequence annotation: CMV
sequence annotation: RGD
sequence: https://synbiohub.org/public/igem/BBa_K404254_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2094681
display id: annotation2094681
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094681/range2094681/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094681/range2094681/1
type: Range
persistent identity: range2094681
display id: range2094681
version: 1
direction: direction/forward
top level: BBa_K404254
start: 1079
end: 2917
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094682
display id: annotation2094682
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094682/range2094682/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094682/range2094682/1
type: Range
persistent identity: range2094682
display id: range2094682
version: 1
direction: direction/forward
top level: BBa_K404254
start: 1274
end: 2917
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094688
display id: annotation2094688
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094688/range2094688/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094688/range2094688/1
type: Range
persistent identity: range2094688
display id: range2094688
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2397
end: 2428
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094680
display id: annotation2094680
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094680/range2094680/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094680/range2094680/1
type: Range
persistent identity: range2094680
display id: range2094680
version: 1
direction: direction/forward
top level: BBa_K404254
start: 671
end: 2917
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094686
display id: annotation2094686
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094686/range2094686/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094686/range2094686/1
type: Range
persistent identity: range2094686
display id: range2094686
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2462
end: 2470
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094685
display id: annotation2094685
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094685/range2094685/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094685/range2094685/1
type: Range
persistent identity: range2094685
display id: range2094685
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2429
end: 2434
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094687
display id: annotation2094687
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094687/range2094687/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094687/range2094687/1
type: Range
persistent identity: range2094687
display id: range2094687
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2471
end: 2486
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094683
display id: annotation2094683
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094683/range2094683/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094683/range2094683/1
type: Range
persistent identity: range2094683
display id: range2094683
version: 1
direction: direction/forward
top level: BBa_K404254
start: 671
end: 3040
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101887
display id: annotation2101887
version: 1
top level: BBa_K404254
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2101887/range2101887/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2101887/range2101887/1
type: Range
persistent identity: range2101887
display id: range2101887
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2433
end: 2435
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094679
display id: annotation2094679
version: 1
top level: BBa_K404254
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094679/range2094679/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094679/range2094679/1
type: Range
persistent identity: range2094679
display id: range2094679
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101886
display id: annotation2101886
version: 1
top level: BBa_K404254
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2101886/range2101886/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2101886/range2101886/1
type: Range
persistent identity: range2101886
display id: range2101886
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2471
end: 2473
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2094684
display id: annotation2094684
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094684/range2094684/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094684/range2094684/1
type: Range
persistent identity: range2094684
display id: range2094684
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2435
end: 2461
orientation: inline

BBa_K883001
type: ComponentDefinition
persistent identity: BBa K883001
display id: BBa_K883001
version: 1
was generated by: iGEM to SBOL conversion
description: CCMV wt coat protein under IPTG induced promoter
creator: Jeroen Bosman
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
group u list: _1146_
m user id: 0
owner id: 12652
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: komt nog
mutable notes: none
mutable provenance: this part is created with a part from the parts registry, and a part made by Wageningen_UR 2012 with the template of Dr. Kormelink
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J04500
component: BBa_K883000
sequence annotation: BBa_J04500
sequence annotation: BBa_K883000
sequence: https://synbiohub.org/public/igem/BBa_K883001_sequence/1

BBa_J04500
type: Component
persistent identity: component2194182
display id: component2194182
version: 1
top level: BBa_K883001
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K883000
type: Component
persistent identity: component2194186
display id: component2194186
version: 1
top level: BBa_K883001
access: public
definition: https://synbiohub.org/public/igem/BBa_K883000/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2194182
display id: annotation2194182
version: 1
top level: BBa_K883001
location: https://synbiohub.org/public/igem/BBa_K883001/annotation2194182/range2194182/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K883001/annotation2194182/range2194182/1
type: Range
persistent identity: range2194182
display id: range2194182
version: 1
direction: direction/reverse
top level: BBa_K883001
start: 1
end: 220
orientation: inline

BBa_K883000
type: SequenceAnnotation
persistent identity: annotation2194186
display id: annotation2194186
version: 1
top level: BBa_K883001
location: https://synbiohub.org/public/igem/BBa_K883001/annotation2194186/range2194186/1
component: BBa_K883000

https://synbiohub.org/public/igem/BBa_K883001/annotation2194186/range2194186/1
type: Range
persistent identity: range2194186
display id: range2194186
version: 1
direction: direction/reverse
top level: BBa_K883001
start: 227
end: 799
orientation: inline

BBa_K404013
type: ComponentDefinition
persistent identity: BBa K404013
display id: BBa_K404013
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP3
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404013_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2097907
display id: annotation2097907
version: 1
top level: BBa_K404013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404013/annotation2097907/range2097907/1

https://synbiohub.org/public/igem/BBa_K404013/annotation2097907/range2097907/1
type: Range
persistent identity: range2097907
display id: range2097907
version: 1
direction: direction/forward
top level: BBa_K404013
start: 670
end: 2305
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097906
display id: annotation2097906
version: 1
top level: BBa_K404013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404013/annotation2097906/range2097906/1

https://synbiohub.org/public/igem/BBa_K404013/annotation2097906/range2097906/1
type: Range
persistent identity: range2097906
display id: range2097906
version: 1
direction: direction/forward
top level: BBa_K404013
start: 2
end: 655
orientation: inline

BBa_K404009
type: ComponentDefinition
persistent identity: BBa K404009
display id: BBa_K404009
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP1
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404009_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2097902
display id: annotation2097902
version: 1
top level: BBa_K404009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404009/annotation2097902/range2097902/1

https://synbiohub.org/public/igem/BBa_K404009/annotation2097902/range2097902/1
type: Range
persistent identity: range2097902
display id: range2097902
version: 1
direction: direction/forward
top level: BBa_K404009
start: 670
end: 2910
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097901
display id: annotation2097901
version: 1
top level: BBa_K404009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404009/annotation2097901/range2097901/1

https://synbiohub.org/public/igem/BBa_K404009/annotation2097901/range2097901/1
type: Range
persistent identity: range2097901
display id: range2097901
version: 1
direction: direction/forward
top level: BBa_K404009
start: 2
end: 655
orientation: inline

BBa_K404235
type: ComponentDefinition
persistent identity: BBa K404235
display id: BBa_K404235
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]Rep-VP123_p5TATAless (ViralBrick 453 Z34C-587 HSPG-KO)
creator: Freiburg Bioware 2010
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: Z34C
sequence annotation: Rep52
sequence annotation: Rep68
sequence annotation: HSPG-KO
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence annotation: Rep78
sequence annotation: p5TATAless
sequence annotation: Rep40
sequence: https://synbiohub.org/public/igem/BBa_K404235_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2098418
display id: annotation2098418
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098418/range2098418/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098418/range2098418/1
type: Range
persistent identity: range2098418
display id: range2098418
version: 1
direction: direction/forward
top level: BBa_K404235
start: 1833
end: 4192
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098420
display id: annotation2098420
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098420/range2098420/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098420/range2098420/1
type: Range
persistent identity: range2098420
display id: range2098420
version: 1
direction: direction/forward
top level: BBa_K404235
start: 2489
end: 4192
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2098421
display id: annotation2098421
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098421/range2098421/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098421/range2098421/1
type: Range
persistent identity: range2098421
display id: range2098421
version: 1
direction: direction/forward
top level: BBa_K404235
start: 3242
end: 3343
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098416
display id: annotation2098416
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098416/range2098416/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098416/range2098416/1
type: Range
persistent identity: range2098416
display id: range2098416
version: 1
direction: direction/forward
top level: BBa_K404235
start: 673
end: 1867
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098415
display id: annotation2098415
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098415/range2098415/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098415/range2098415/1
type: Range
persistent identity: range2098415
display id: range2098415
version: 1
direction: direction/forward
top level: BBa_K404235
start: 2
end: 1933
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101872
display id: annotation2101872
version: 1
top level: BBa_K404235
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2101872/range2101872/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2101872/range2101872/1
type: Range
persistent identity: range2101872
display id: range2101872
version: 1
direction: direction/forward
top level: BBa_K404235
start: 3155
end: 3157
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2098425
display id: annotation2098425
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098425/range2098425/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098425/range2098425/1
type: Range
persistent identity: range2098425
display id: range2098425
version: 1
direction: direction/forward
top level: BBa_K404235
start: 1833
end: 4315
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098419
display id: annotation2098419
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098419/range2098419/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098419/range2098419/1
type: Range
persistent identity: range2098419
display id: range2098419
version: 1
direction: direction/forward
top level: BBa_K404235
start: 2294
end: 4192
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101871
display id: annotation2101871
version: 1
top level: BBa_K404235
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2101871/range2101871/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2101871/range2101871/1
type: Range
persistent identity: range2101871
display id: range2101871
version: 1
direction: direction/forward
top level: BBa_K404235
start: 3377
end: 3379
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098414
display id: annotation2098414
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098414/range2098414/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098414/range2098414/1
type: Range
persistent identity: range2098414
display id: range2098414
version: 1
direction: direction/forward
top level: BBa_K404235
start: 2
end: 1867
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098423
display id: annotation2098423
version: 1
top level: BBa_K404235
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098423/range2098423/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098423/range2098423/1
type: Range
persistent identity: range2098423
display id: range2098423
version: 1
direction: direction/forward
top level: BBa_K404235
start: 4324
end: 4487
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098417
display id: annotation2098417
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098417/range2098417/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098417/range2098417/1
type: Range
persistent identity: range2098417
display id: range2098417
version: 1
direction: direction/forward
top level: BBa_K404235
start: 673
end: 1933
orientation: inline

BBa_K404222
type: ComponentDefinition
persistent identity: BBa K404222
display id: BBa_K404222
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-453-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: A-spacer
sequence annotation: VP2
sequence annotation: His-tag
sequence annotation: Rep52
sequence annotation: VP3
sequence annotation: VP1
sequence annotation: Rep78
sequence annotation: p5TATAless
sequence annotation: Rep68
sequence annotation: AAV-a-cap (Stratagene)
sequence annotation: Rep40
sequence: https://synbiohub.org/public/igem/BBa_K404222_sequence/1

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098261
display id: annotation2098261
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098261/range2098261/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098261/range2098261/1
type: Range
persistent identity: range2098261
display id: range2098261
version: 1
direction: direction/forward
top level: BBa_K404222
start: 3242
end: 3244
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098256
display id: annotation2098256
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098256/range2098256/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098256/range2098256/1
type: Range
persistent identity: range2098256
display id: range2098256
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2294
end: 4114
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2098260
display id: annotation2098260
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098260/range2098260/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098260/range2098260/1
type: Range
persistent identity: range2098260
display id: range2098260
version: 1
direction: direction/forward
top level: BBa_K404222
start: 3245
end: 3262
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098254
display id: annotation2098254
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098254/range2098254/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098254/range2098254/1
type: Range
persistent identity: range2098254
display id: range2098254
version: 1
direction: direction/forward
top level: BBa_K404222
start: 673
end: 1867
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098262
display id: annotation2098262
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098262/range2098262/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098262/range2098262/1
type: Range
persistent identity: range2098262
display id: range2098262
version: 1
direction: direction/forward
top level: BBa_K404222
start: 3263
end: 3265
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098257
display id: annotation2098257
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098257/range2098257/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098257/range2098257/1
type: Range
persistent identity: range2098257
display id: range2098257
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2489
end: 4114
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098255
display id: annotation2098255
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098255/range2098255/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098255/range2098255/1
type: Range
persistent identity: range2098255
display id: range2098255
version: 1
direction: direction/forward
top level: BBa_K404222
start: 1883
end: 4114
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098251
display id: annotation2098251
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098251/range2098251/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098251/range2098251/1
type: Range
persistent identity: range2098251
display id: range2098251
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2
end: 1867
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098258
display id: annotation2098258
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098258/range2098258/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098258/range2098258/1
type: Range
persistent identity: range2098258
display id: range2098258
version: 1
direction: direction/forward
top level: BBa_K404222
start: 4246
end: 4409
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098252
display id: annotation2098252
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098252/range2098252/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098252/range2098252/1
type: Range
persistent identity: range2098252
display id: range2098252
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2
end: 1933
orientation: inline

AAV-a-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2098259
display id: annotation2098259
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098259/range2098259/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098259/range2098259/1
type: Range
persistent identity: range2098259
display id: range2098259
version: 1
direction: direction/forward
top level: BBa_K404222
start: 1883
end: 4237
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098253
display id: annotation2098253
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098253/range2098253/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098253/range2098253/1
type: Range
persistent identity: range2098253
display id: range2098253
version: 1
direction: direction/forward
top level: BBa_K404222
start: 673
end: 1933
orientation: inline

BBa_K404118
type: ComponentDefinition
persistent identity: BBa K404118
display id: BBa_K404118
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_phTERT_betaglobin
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: phTERT
sequence annotation: left ITR
sequence annotation: Beta-Globin-intron
sequence: https://synbiohub.org/public/igem/BBa_K404118_sequence/1

phTERT
type: SequenceAnnotation
persistent identity: annotation2093235
display id: annotation2093235
version: 1
top level: BBa_K404118
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404118/annotation2093235/range2093235/1

https://synbiohub.org/public/igem/BBa_K404118/annotation2093235/range2093235/1
type: Range
persistent identity: range2093235
display id: range2093235
version: 1
direction: direction/forward
top level: BBa_K404118
start: 154
end: 612
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093233
display id: annotation2093233
version: 1
top level: BBa_K404118
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404118/annotation2093233/range2093233/1

https://synbiohub.org/public/igem/BBa_K404118/annotation2093233/range2093233/1
type: Range
persistent identity: range2093233
display id: range2093233
version: 1
direction: direction/forward
top level: BBa_K404118
start: 1
end: 147
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093236
display id: annotation2093236
version: 1
top level: BBa_K404118
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404118/annotation2093236/range2093236/1

https://synbiohub.org/public/igem/BBa_K404118/annotation2093236/range2093236/1
type: Range
persistent identity: range2093236
display id: range2093236
version: 1
direction: direction/forward
top level: BBa_K404118
start: 619
end: 1113
orientation: inline

BBa_K404167
type: ComponentDefinition
persistent identity: BBa K404167
display id: BBa_K404167
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_VP1up_NLS_mVenus_VP23(ViralBrick-587-KO-BAP)
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:24Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Freiburg Bioware 2010
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: CMV
sequence annotation: HSPG-KO
sequence annotation: BAP
sequence annotation: upstream 587
sequence annotation: mVenus
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: GLS-spacer
sequence annotation: downstream 587
sequence annotation: NLS
sequence annotation: VP1up
sequence annotation: TG-spacer
sequence: https://synbiohub.org/public/igem/BBa_K404167_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2095315
display id: annotation2095315
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095315/range2095315/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095315/range2095315/1
type: Range
persistent identity: range2095315
display id: range2095315
version: 1
top level: BBa_K404167
direction: direction/forward
start: 2027
end: 3688
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095314
display id: annotation2095314
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095314/range2095314/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095314/range2095314/1
type: Range
persistent identity: range2095314
display id: range2095314
version: 1
top level: BBa_K404167
direction: direction/forward
start: 1835
end: 3688
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095311
display id: annotation2095311
version: 1
top level: BBa_K404167
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095311/range2095311/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095311/range2095311/1
type: Range
persistent identity: range2095311
display id: range2095311
version: 1
top level: BBa_K404167
direction: direction/forward
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101843
display id: annotation2101843
version: 1
top level: BBa_K404167
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2101843/range2101843/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2101843/range2101843/1
type: Range
persistent identity: range2101843
display id: range2101843
version: 1
top level: BBa_K404167
direction: direction/forward
start: 3172
end: 3174
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2095317
display id: annotation2095317
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095317/range2095317/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095317/range2095317/1
type: Range
persistent identity: range2095317
display id: range2095317
version: 1
top level: BBa_K404167
direction: direction/forward
start: 3188
end: 3232
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2095321
display id: annotation2095321
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095321/range2095321/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095321/range2095321/1
type: Range
persistent identity: range2095321
display id: range2095321
version: 1
top level: BBa_K404167
direction: direction/forward
start: 3150
end: 3181
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2095313
display id: annotation2095313
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095313/range2095313/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095313/range2095313/1
type: Range
persistent identity: range2095313
display id: range2095313
version: 1
top level: BBa_K404167
direction: direction/forward
start: 1115
end: 1828
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095316
display id: annotation2095316
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095316/range2095316/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095316/range2095316/1
type: Range
persistent identity: range2095316
display id: range2095316
version: 1
top level: BBa_K404167
direction: direction/forward
start: 1835
end: 3811
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2095319
display id: annotation2095319
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095319/range2095319/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095319/range2095319/1
type: Range
persistent identity: range2095319
display id: range2095319
version: 1
top level: BBa_K404167
direction: direction/forward
start: 3233
end: 3241
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2095320
display id: annotation2095320
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095320/range2095320/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095320/range2095320/1
type: Range
persistent identity: range2095320
display id: range2095320
version: 1
top level: BBa_K404167
direction: direction/forward
start: 3242
end: 3257
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2095310
display id: annotation2095310
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095310/range2095310/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095310/range2095310/1
type: Range
persistent identity: range2095310
display id: range2095310
version: 1
top level: BBa_K404167
direction: direction/forward
start: 1088
end: 1108
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101842
display id: annotation2101842
version: 1
top level: BBa_K404167
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2101842/range2101842/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2101842/range2101842/1
type: Range
persistent identity: range2101842
display id: range2101842
version: 1
top level: BBa_K404167
direction: direction/forward
start: 3234
end: 3236
orientation: inline

VP1up
type: SequenceAnnotation
persistent identity: annotation2095312
display id: annotation2095312
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095312/range2095312/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095312/range2095312/1
type: Range
persistent identity: range2095312
display id: range2095312
version: 1
top level: BBa_K404167
direction: direction/forward
start: 673
end: 1081
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2095318
display id: annotation2095318
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095318/range2095318/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095318/range2095318/1
type: Range
persistent identity: range2095318
display id: range2095318
version: 1
top level: BBa_K404167
direction: direction/forward
start: 3182
end: 3187
orientation: inline

BBa_K404003
type: ComponentDefinition
persistent identity: BBa K404003
display id: BBa_K404003
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123(ViralBrick-587KO-empty)_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: p5TATAless
sequence annotation: basic region 3
sequence annotation: A20-1
sequence annotation: basic region 4
sequence annotation: AAV-2-cap
sequence annotation: HSPG-KO
sequence annotation: Rep68
sequence annotation: scar
sequence annotation: basic region 2
sequence annotation: A20-2
sequence annotation: p40
sequence annotation: VP3
sequence annotation: basic region 1
sequence annotation: Rep78
sequence annotation: p19
sequence annotation: VP1
sequence annotation: PLA2
sequence annotation: B1
sequence annotation: Rep52
sequence annotation: Rep40
sequence annotation: VP2
sequence annotation: AAP
sequence annotation: A20-4
sequence: https://synbiohub.org/public/igem/BBa_K404003_sequence/1

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2102712
display id: annotation2102712
version: 1
top level: BBa_K404003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102712/range2102712/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102712/range2102712/1
type: Range
persistent identity: range2102712
display id: range2102712
version: 1
direction: direction/forward
top level: BBa_K404003
start: 4222
end: 4385
orientation: inline

basic region 3
type: SequenceAnnotation
persistent identity: annotation2102704
display id: annotation2102704
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102704/range2102704/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102704/range2102704/1
type: Range
persistent identity: range2102704
display id: range2102704
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2378
end: 2398
orientation: inline

A20-1
type: SequenceAnnotation
persistent identity: annotation2102706
display id: annotation2102706
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102706/range2102706/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102706/range2102706/1
type: Range
persistent identity: range2102706
display id: range2102706
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2693
end: 2722
orientation: inline

basic region 4
type: SequenceAnnotation
persistent identity: annotation2102707
display id: annotation2102707
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102707/range2102707/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102707/range2102707/1
type: Range
persistent identity: range2102707
display id: range2102707
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2801
end: 2818
orientation: inline

AAV-2-cap
type: SequenceAnnotation
persistent identity: annotation2102749
display id: annotation2102749
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102749/range2102749/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102749/range2102749/1
type: Range
persistent identity: range2102749
display id: range2102749
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3694
end: 4213
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2102711
display id: annotation2102711
version: 1
top level: BBa_K404003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102711/range2102711/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102711/range2102711/1
type: Range
persistent identity: range2102711
display id: range2102711
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3644
end: 3646
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2102694
display id: annotation2102694
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102694/range2102694/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102694/range2102694/1
type: Range
persistent identity: range2102694
display id: range2102694
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1
end: 1933
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2102750
display id: annotation2102750
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102750/range2102750/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102750/range2102750/1
type: Range
persistent identity: range2102750
display id: range2102750
version: 1
direction: direction/forward
top level: BBa_K404003
start: 4214
end: 4221
orientation: inline

basic region 2
type: SequenceAnnotation
persistent identity: annotation2102703
display id: annotation2102703
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102703/range2102703/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102703/range2102703/1
type: Range
persistent identity: range2102703
display id: range2102703
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2300
end: 2320
orientation: inline

A20-2
type: SequenceAnnotation
persistent identity: annotation2102708
display id: annotation2102708
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102708/range2102708/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102708/range2102708/1
type: Range
persistent identity: range2102708
display id: range2102708
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2990
end: 3016
orientation: inline

p40
type: SequenceAnnotation
persistent identity: annotation2102698
display id: annotation2102698
version: 1
top level: BBa_K404003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102698/range2102698/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102698/range2102698/1
type: Range
persistent identity: range2102698
display id: range2102698
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1390
end: 1507
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2102705
display id: annotation2102705
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102705/range2102705/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102705/range2102705/1
type: Range
persistent identity: range2102705
display id: range2102705
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2489
end: 4090
orientation: inline

basic region 1
type: SequenceAnnotation
persistent identity: annotation2102701
display id: annotation2102701
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102701/range2102701/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102701/range2102701/1
type: Range
persistent identity: range2102701
display id: range2102701
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2240
end: 2260
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2102693
display id: annotation2102693
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102693/range2102693/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102693/range2102693/1
type: Range
persistent identity: range2102693
display id: range2102693
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1
end: 1867
orientation: inline

p19
type: SequenceAnnotation
persistent identity: annotation2102695
display id: annotation2102695
version: 1
top level: BBa_K404003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102695/range2102695/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102695/range2102695/1
type: Range
persistent identity: range2102695
display id: range2102695
version: 1
direction: direction/forward
top level: BBa_K404003
start: 410
end: 529
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2102699
display id: annotation2102699
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102699/range2102699/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102699/range2102699/1
type: Range
persistent identity: range2102699
display id: range2102699
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1833
end: 4090
orientation: inline

PLA2
type: SequenceAnnotation
persistent identity: annotation2102700
display id: annotation2102700
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102700/range2102700/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102700/range2102700/1
type: Range
persistent identity: range2102700
display id: range2102700
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2015
end: 2191
orientation: inline

B1
type: SequenceAnnotation
persistent identity: annotation2102713
display id: annotation2102713
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102713/range2102713/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102713/range2102713/1
type: Range
persistent identity: range2102713
display id: range2102713
version: 1
direction: direction/forward
top level: BBa_K404003
start: 4058
end: 4081
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2102696
display id: annotation2102696
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102696/range2102696/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102696/range2102696/1
type: Range
persistent identity: range2102696
display id: range2102696
version: 1
direction: direction/forward
top level: BBa_K404003
start: 673
end: 1867
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2102697
display id: annotation2102697
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102697/range2102697/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102697/range2102697/1
type: Range
persistent identity: range2102697
display id: range2102697
version: 1
direction: direction/forward
top level: BBa_K404003
start: 673
end: 1933
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2102710
display id: annotation2102710
version: 1
top level: BBa_K404003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102710/range2102710/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102710/range2102710/1
type: Range
persistent identity: range2102710
display id: range2102710
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3635
end: 3637
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2102702
display id: annotation2102702
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102702/range2102702/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102702/range2102702/1
type: Range
persistent identity: range2102702
display id: range2102702
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2294
end: 4090
orientation: inline

AAP
type: SequenceAnnotation
persistent identity: annotation2102747
display id: annotation2102747
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102747/range2102747/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102747/range2102747/1
type: Range
persistent identity: range2102747
display id: range2102747
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2409
end: 3023
orientation: inline

A20-4
type: SequenceAnnotation
persistent identity: annotation2102709
display id: annotation2102709
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102709/range2102709/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102709/range2102709/1
type: Range
persistent identity: range2102709
display id: range2102709
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3578
end: 3607
orientation: inline

BBa_K404005
type: ComponentDefinition
persistent identity: BBa K404005
display id: BBa_K404005
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP23(ViralBrick-587KO-empty)_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep40
sequence annotation: p5TATAless
sequence annotation: VP3
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: Rep68
sequence annotation: Rep52
sequence annotation: Rep78
sequence: https://synbiohub.org/public/igem/BBa_K404005_sequence/1

Rep40
type: SequenceAnnotation
persistent identity: annotation2097886
display id: annotation2097886
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097886/range2097886/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097886/range2097886/1
type: Range
persistent identity: range2097886
display id: range2097886
version: 1
direction: direction/forward
top level: BBa_K404005
start: 673
end: 1933
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2097890
display id: annotation2097890
version: 1
top level: BBa_K404005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097890/range2097890/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097890/range2097890/1
type: Range
persistent identity: range2097890
display id: range2097890
version: 1
direction: direction/forward
top level: BBa_K404005
start: 4238
end: 4401
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097889
display id: annotation2097889
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097889/range2097889/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097889/range2097889/1
type: Range
persistent identity: range2097889
display id: range2097889
version: 1
direction: direction/forward
top level: BBa_K404005
start: 2489
end: 4090
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101714
display id: annotation2101714
version: 1
top level: BBa_K404005
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2101714/range2101714/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2101714/range2101714/1
type: Range
persistent identity: range2101714
display id: range2101714
version: 1
direction: direction/forward
top level: BBa_K404005
start: 3635
end: 3637
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097888
display id: annotation2097888
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097888/range2097888/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097888/range2097888/1
type: Range
persistent identity: range2097888
display id: range2097888
version: 1
direction: direction/forward
top level: BBa_K404005
start: 2294
end: 4090
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2097884
display id: annotation2097884
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097884/range2097884/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097884/range2097884/1
type: Range
persistent identity: range2097884
display id: range2097884
version: 1
direction: direction/forward
top level: BBa_K404005
start: 1
end: 1867
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101713
display id: annotation2101713
version: 1
top level: BBa_K404005
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2101713/range2101713/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2101713/range2101713/1
type: Range
persistent identity: range2101713
display id: range2101713
version: 1
direction: direction/forward
top level: BBa_K404005
start: 3644
end: 3646
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2097887
display id: annotation2097887
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097887/range2097887/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097887/range2097887/1
type: Range
persistent identity: range2097887
display id: range2097887
version: 1
direction: direction/forward
top level: BBa_K404005
start: 673
end: 1867
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2097885
display id: annotation2097885
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097885/range2097885/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097885/range2097885/1
type: Range
persistent identity: range2097885
display id: range2097885
version: 1
direction: direction/forward
top level: BBa_K404005
start: 1
end: 1933
orientation: inline

BBa_K404103
type: ComponentDefinition
persistent identity: BBa K404103
display id: BBa_K404103
version: 1
was generated by: iGEM to SBOL conversion
description: p5 promoter
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: p5 promoter
sequence: https://synbiohub.org/public/igem/BBa_K404103_sequence/1

p5 promoter
type: SequenceAnnotation
persistent identity: annotation2093169
display id: annotation2093169
version: 1
top level: BBa_K404103
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404103/annotation2093169/range2093169/1

https://synbiohub.org/public/igem/BBa_K404103/annotation2093169/range2093169/1
type: Range
persistent identity: range2093169
display id: range2093169
version: 1
direction: direction/forward
top level: BBa_K404103
start: 1
end: 166
orientation: inline

BBa_K404305
type: ComponentDefinition
persistent identity: BBa K404305
display id: BBa_K404305
version: 1
was generated by: iGEM to SBOL conversion
description: Z-EGFR-1907_Long-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Z-EGFR-1907
sequence annotation: Long-linker
sequence: https://synbiohub.org/public/igem/BBa_K404305_sequence/1

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094756
display id: annotation2094756
version: 1
top level: BBa_K404305
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404305/annotation2094756/range2094756/1

https://synbiohub.org/public/igem/BBa_K404305/annotation2094756/range2094756/1
type: Range
persistent identity: range2094756
display id: range2094756
version: 1
direction: direction/forward
top level: BBa_K404305
start: 1
end: 175
orientation: inline

Long-linker
type: SequenceAnnotation
persistent identity: annotation2094757
display id: annotation2094757
version: 1
top level: BBa_K404305
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404305/annotation2094757/range2094757/1

https://synbiohub.org/public/igem/BBa_K404305/annotation2094757/range2094757/1
type: Range
persistent identity: range2094757
display id: range2094757
version: 1
direction: direction/forward
top level: BBa_K404305
start: 181
end: 216
orientation: inline

BBa_K404157
type: ComponentDefinition
persistent identity: BBa K404157
display id: BBa_K404157
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_Z-EGFR-1907_Long-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: HSPG-KO
sequence annotation: long-linker
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: CMV
sequence annotation: ZEGFR_1907
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404157_sequence/1

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101792
display id: annotation2101792
version: 1
top level: BBa_K404157
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2101792/range2101792/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2101792/range2101792/1
type: Range
persistent identity: range2101792
display id: range2101792
version: 1
direction: direction/forward
top level: BBa_K404157
start: 2240
end: 2242
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101793
display id: annotation2101793
version: 1
top level: BBa_K404157
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2101793/range2101793/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2101793/range2101793/1
type: Range
persistent identity: range2101793
display id: range2101793
version: 1
direction: direction/forward
top level: BBa_K404157
start: 2231
end: 2233
orientation: inline

long-linker
type: SequenceAnnotation
persistent identity: annotation2094996
display id: annotation2094996
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094996/range2094996/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094996/range2094996/1
type: Range
persistent identity: range2094996
display id: range2094996
version: 1
direction: direction/forward
top level: BBa_K404157
start: 851
end: 886
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094998
display id: annotation2094998
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094998/range2094998/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094998/range2094998/1
type: Range
persistent identity: range2094998
display id: range2094998
version: 1
direction: direction/forward
top level: BBa_K404157
start: 1085
end: 2686
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094999
display id: annotation2094999
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094999/range2094999/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094999/range2094999/1
type: Range
persistent identity: range2094999
display id: range2094999
version: 1
direction: direction/forward
top level: BBa_K404157
start: 893
end: 2809
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094994
display id: annotation2094994
version: 1
top level: BBa_K404157
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094994/range2094994/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094994/range2094994/1
type: Range
persistent identity: range2094994
display id: range2094994
version: 1
direction: direction/forward
top level: BBa_K404157
start: 2
end: 655
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2094995
display id: annotation2094995
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094995/range2094995/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094995/range2094995/1
type: Range
persistent identity: range2094995
display id: range2094995
version: 1
direction: direction/forward
top level: BBa_K404157
start: 671
end: 844
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094997
display id: annotation2094997
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094997/range2094997/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094997/range2094997/1
type: Range
persistent identity: range2094997
display id: range2094997
version: 1
direction: direction/forward
top level: BBa_K404157
start: 893
end: 2686
orientation: inline

BBa_K404303
type: ComponentDefinition
persistent identity: BBa K404303
display id: BBa_K404303
version: 1
was generated by: iGEM to SBOL conversion
description: Z-EGFR-1907_Short-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Short-linker
sequence annotation: Z-EGFR-1907
sequence: https://synbiohub.org/public/igem/BBa_K404303_sequence/1

Short-linker
type: SequenceAnnotation
persistent identity: annotation2094743
display id: annotation2094743
version: 1
top level: BBa_K404303
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404303/annotation2094743/range2094743/1

https://synbiohub.org/public/igem/BBa_K404303/annotation2094743/range2094743/1
type: Range
persistent identity: range2094743
display id: range2094743
version: 1
direction: direction/forward
top level: BBa_K404303
start: 181
end: 192
orientation: inline

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094742
display id: annotation2094742
version: 1
top level: BBa_K404303
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404303/annotation2094742/range2094742/1

https://synbiohub.org/public/igem/BBa_K404303/annotation2094742/range2094742/1
type: Range
persistent identity: range2094742
display id: range2094742
version: 1
direction: direction/forward
top level: BBa_K404303
start: 1
end: 175
orientation: inline

BBa_K404226
type: ComponentDefinition
persistent identity: BBa K404226
display id: BBa_K404226
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-587-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404212
type: ComponentDefinition
persistent identity: BBa K404212
display id: BBa_K404212
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-587KO-RGD
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RGD-motif
sequence annotation: R=>A
sequence annotation: downstream 453
sequence annotation: upstream 453
sequence annotation: upstream 587KO
sequence annotation: GLS Spacer
sequence annotation: TG Spacer
sequence annotation: downstream 587KO
sequence: https://synbiohub.org/public/igem/BBa_K404212_sequence/1

RGD-motif
type: SequenceAnnotation
persistent identity: annotation2079145
display id: annotation2079145
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079145/range2079145/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079145/range2079145/1
type: Range
persistent identity: range2079145
display id: range2079145
version: 1
direction: direction/forward
top level: BBa_K404212
start: 49
end: 75
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079142
display id: annotation2079142
version: 1
top level: BBa_K404212
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079142/range2079142/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079142/range2079142/1
type: Range
persistent identity: range2079142
display id: range2079142
version: 1
direction: direction/forward
top level: BBa_K404212
start: 34
end: 36
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079148
display id: annotation2079148
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079148/range2079148/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079148/range2079148/1
type: Range
persistent identity: range2079148
display id: range2079148
version: 1
direction: direction/forward
top level: BBa_K404212
start: 106
end: 114
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079140
display id: annotation2079140
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079140/range2079140/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079140/range2079140/1
type: Range
persistent identity: range2079140
display id: range2079140
version: 1
direction: direction/forward
top level: BBa_K404212
start: 1
end: 9
orientation: inline

upstream 587KO
type: SequenceAnnotation
persistent identity: annotation2079141
display id: annotation2079141
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079141/range2079141/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079141/range2079141/1
type: Range
persistent identity: range2079141
display id: range2079141
version: 1
direction: direction/forward
top level: BBa_K404212
start: 10
end: 42
orientation: inline

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2079146
display id: annotation2079146
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079146/range2079146/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079146/range2079146/1
type: Range
persistent identity: range2079146
display id: range2079146
version: 1
direction: direction/forward
top level: BBa_K404212
start: 76
end: 87
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2079143
display id: annotation2079143
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079143/range2079143/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079143/range2079143/1
type: Range
persistent identity: range2079143
display id: range2079143
version: 1
direction: direction/forward
top level: BBa_K404212
start: 43
end: 48
orientation: inline

downstream 587KO
type: SequenceAnnotation
persistent identity: annotation2079147
display id: annotation2079147
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079147/range2079147/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079147/range2079147/1
type: Range
persistent identity: range2079147
display id: range2079147
version: 1
direction: direction/forward
top level: BBa_K404212
start: 85
end: 105
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079144
display id: annotation2079144
version: 1
top level: BBa_K404212
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079144/range2079144/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079144/range2079144/1
type: Range
persistent identity: range2079144
display id: range2079144
version: 1
direction: direction/forward
top level: BBa_K404212
start: 85
end: 87
orientation: inline

BBa_K404228
type: ComponentDefinition
persistent identity: BBa K404228
display id: BBa_K404228
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-587-BLA)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404304
type: ComponentDefinition
persistent identity: BBa K404304
display id: BBa_K404304
version: 1
was generated by: iGEM to SBOL conversion
description: Z-EGFR-1907_Middle-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Middle-linker
sequence annotation: Z-EGFR-1907
sequence: https://synbiohub.org/public/igem/BBa_K404304_sequence/1

Middle-linker
type: SequenceAnnotation
persistent identity: annotation2094746
display id: annotation2094746
version: 1
top level: BBa_K404304
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404304/annotation2094746/range2094746/1

https://synbiohub.org/public/igem/BBa_K404304/annotation2094746/range2094746/1
type: Range
persistent identity: range2094746
display id: range2094746
version: 1
direction: direction/forward
top level: BBa_K404304
start: 181
end: 204
orientation: inline

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094745
display id: annotation2094745
version: 1
top level: BBa_K404304
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404304/annotation2094745/range2094745/1

https://synbiohub.org/public/igem/BBa_K404304/annotation2094745/range2094745/1
type: Range
persistent identity: range2094745
display id: range2094745
version: 1
direction: direction/forward
top level: BBa_K404304
start: 1
end: 175
orientation: inline

BBa_K404168
type: ComponentDefinition
persistent identity: BBa K404168
display id: BBa_K404168
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_VP1up_NLS_mVenus_VP2/3(ViralBrick-587-KO-His)
creator: Freiburg Bioware 2010
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: HSPG-KO
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: NLS
sequence annotation: mVenus
sequence annotation: VP2
sequence annotation: downstream 587
sequence annotation: TG-spacer
sequence annotation: upstream 587
sequence annotation: VP1up
sequence annotation: GLS-spacer
sequence annotation: VP3
sequence annotation: CMV
sequence annotation: His-tag
sequence: https://synbiohub.org/public/igem/BBa_K404168_sequence/1

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101845
display id: annotation2101845
version: 1
top level: BBa_K404168
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2101845/range2101845/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2101845/range2101845/1
type: Range
persistent identity: range2101845
display id: range2101845
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3163
end: 3165
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095294
display id: annotation2095294
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095294/range2095294/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095294/range2095294/1
type: Range
persistent identity: range2095294
display id: range2095294
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1835
end: 3784
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2095284
display id: annotation2095284
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095284/range2095284/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095284/range2095284/1
type: Range
persistent identity: range2095284
display id: range2095284
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1088
end: 1108
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2095282
display id: annotation2095282
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095282/range2095282/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095282/range2095282/1
type: Range
persistent identity: range2095282
display id: range2095282
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1115
end: 1828
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095285
display id: annotation2095285
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095285/range2095285/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095285/range2095285/1
type: Range
persistent identity: range2095285
display id: range2095285
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1835
end: 3661
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2095292
display id: annotation2095292
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095292/range2095292/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095292/range2095292/1
type: Range
persistent identity: range2095292
display id: range2095292
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3215
end: 3230
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2095288
display id: annotation2095288
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095288/range2095288/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095288/range2095288/1
type: Range
persistent identity: range2095288
display id: range2095288
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3182
end: 3187
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2095293
display id: annotation2095293
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095293/range2095293/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095293/range2095293/1
type: Range
persistent identity: range2095293
display id: range2095293
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3150
end: 3181
orientation: inline

VP1up
type: SequenceAnnotation
persistent identity: annotation2095281
display id: annotation2095281
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095281/range2095281/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095281/range2095281/1
type: Range
persistent identity: range2095281
display id: range2095281
version: 1
direction: direction/forward
top level: BBa_K404168
start: 673
end: 1081
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2095290
display id: annotation2095290
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095290/range2095290/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095290/range2095290/1
type: Range
persistent identity: range2095290
display id: range2095290
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3206
end: 3214
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095286
display id: annotation2095286
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095286/range2095286/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095286/range2095286/1
type: Range
persistent identity: range2095286
display id: range2095286
version: 1
direction: direction/forward
top level: BBa_K404168
start: 2027
end: 3661
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101844
display id: annotation2101844
version: 1
top level: BBa_K404168
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2101844/range2101844/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2101844/range2101844/1
type: Range
persistent identity: range2101844
display id: range2101844
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3215
end: 3217
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095279
display id: annotation2095279
version: 1
top level: BBa_K404168
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095279/range2095279/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095279/range2095279/1
type: Range
persistent identity: range2095279
display id: range2095279
version: 1
direction: direction/forward
top level: BBa_K404168
start: 2
end: 655
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2095287
display id: annotation2095287
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095287/range2095287/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095287/range2095287/1
type: Range
persistent identity: range2095287
display id: range2095287
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3188
end: 3205
orientation: inline

BBa_K404202
type: ComponentDefinition
persistent identity: BBa K404202
display id: BBa_K404202
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-453-His-Tag
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstream 453
sequence annotation: His-Tag
sequence annotation: A Spacer
sequence annotation: downstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404202_sequence/1

upstream 453
type: SequenceAnnotation
persistent identity: annotation2078934
display id: annotation2078934
version: 1
top level: BBa_K404202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404202/annotation2078934/range2078934/1

https://synbiohub.org/public/igem/BBa_K404202/annotation2078934/range2078934/1
type: Range
persistent identity: range2078934
display id: range2078934
version: 1
direction: direction/forward
top level: BBa_K404202
start: 1
end: 42
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2078936
display id: annotation2078936
version: 1
top level: BBa_K404202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404202/annotation2078936/range2078936/1

https://synbiohub.org/public/igem/BBa_K404202/annotation2078936/range2078936/1
type: Range
persistent identity: range2078936
display id: range2078936
version: 1
direction: direction/forward
top level: BBa_K404202
start: 46
end: 63
orientation: inline

A Spacer
type: SequenceAnnotation
persistent identity: annotation2078937
display id: annotation2078937
version: 1
top level: BBa_K404202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404202/annotation2078937/range2078937/1

https://synbiohub.org/public/igem/BBa_K404202/annotation2078937/range2078937/1
type: Range
persistent identity: range2078937
display id: range2078937
version: 1
direction: direction/forward
top level: BBa_K404202
start: 64
end: 66
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2078938
display id: annotation2078938
version: 1
top level: BBa_K404202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404202/annotation2078938/range2078938/1

https://synbiohub.org/public/igem/BBa_K404202/annotation2078938/range2078938/1
type: Range
persistent identity: range2078938
display id: range2078938
version: 1
direction: direction/forward
top level: BBa_K404202
start: 67
end: 87
orientation: inline

A Spacer
type: SequenceAnnotation
persistent identity: annotation2078935
display id: annotation2078935
version: 1
top level: BBa_K404202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404202/annotation2078935/range2078935/1

https://synbiohub.org/public/igem/BBa_K404202/annotation2078935/range2078935/1
type: Range
persistent identity: range2078935
display id: range2078935
version: 1
direction: direction/forward
top level: BBa_K404202
start: 43
end: 45
orientation: inline

BBa_K404255
type: ComponentDefinition
persistent identity: BBa K404255
display id: BBa_K404255
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Z34C)
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: CMV
sequence annotation: Z34C
sequence annotation: upstream 587
sequence annotation: VP2
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: downstream 587
sequence annotation: VP1
sequence annotation: HSPG-KO
sequence: https://synbiohub.org/public/igem/BBa_K404255_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2094721
display id: annotation2094721
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094721/range2094721/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094721/range2094721/1
type: Range
persistent identity: range2094721
display id: range2094721
version: 1
direction: direction/forward
top level: BBa_K404255
start: 1274
end: 2977
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094718
display id: annotation2094718
version: 1
top level: BBa_K404255
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094718/range2094718/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094718/range2094718/1
type: Range
persistent identity: range2094718
display id: range2094718
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2
end: 655
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2094723
display id: annotation2094723
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094723/range2094723/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094723/range2094723/1
type: Range
persistent identity: range2094723
display id: range2094723
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2429
end: 2530
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094724
display id: annotation2094724
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094724/range2094724/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094724/range2094724/1
type: Range
persistent identity: range2094724
display id: range2094724
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2397
end: 2428
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094720
display id: annotation2094720
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094720/range2094720/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094720/range2094720/1
type: Range
persistent identity: range2094720
display id: range2094720
version: 1
direction: direction/forward
top level: BBa_K404255
start: 1079
end: 2977
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094722
display id: annotation2094722
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094722/range2094722/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094722/range2094722/1
type: Range
persistent identity: range2094722
display id: range2094722
version: 1
direction: direction/forward
top level: BBa_K404255
start: 671
end: 3100
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094725
display id: annotation2094725
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094725/range2094725/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094725/range2094725/1
type: Range
persistent identity: range2094725
display id: range2094725
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2531
end: 2546
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094719
display id: annotation2094719
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094719/range2094719/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094719/range2094719/1
type: Range
persistent identity: range2094719
display id: range2094719
version: 1
direction: direction/forward
top level: BBa_K404255
start: 671
end: 2977
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101892
display id: annotation2101892
version: 1
top level: BBa_K404255
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2101892/range2101892/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2101892/range2101892/1
type: Range
persistent identity: range2101892
display id: range2101892
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2531
end: 2533
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101893
display id: annotation2101893
version: 1
top level: BBa_K404255
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2101893/range2101893/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2101893/range2101893/1
type: Range
persistent identity: range2101893
display id: range2101893
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2420
end: 2422
orientation: inline

BBa_K404229
type: ComponentDefinition
persistent identity: BBa K404229
display id: BBa_K404229
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-BAP)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: HSPG-KO
sequence annotation: VP3
sequence annotation: p5TATAless
sequence annotation: Rep40
sequence annotation: Rep52
sequence annotation: BAP
sequence annotation: GLS-spacer
sequence annotation: upstream 587
sequence annotation: VP2
sequence annotation: downstream 587
sequence annotation: Rep78
sequence annotation: Rep68
sequence annotation: TG-spacer
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404229_sequence/1

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101846
display id: annotation2101846
version: 1
top level: BBa_K404229
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2101846/range2101846/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2101846/range2101846/1
type: Range
persistent identity: range2101846
display id: range2101846
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3696
end: 3698
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098861
display id: annotation2098861
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098861/range2098861/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098861/range2098861/1
type: Range
persistent identity: range2098861
display id: range2098861
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2489
end: 4150
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098912
display id: annotation2098912
version: 1
top level: BBa_K404229
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098912/range2098912/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098912/range2098912/1
type: Range
persistent identity: range2098912
display id: range2098912
version: 1
direction: direction/forward
top level: BBa_K404229
start: 4282
end: 4445
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098709
display id: annotation2098709
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098709/range2098709/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098709/range2098709/1
type: Range
persistent identity: range2098709
display id: range2098709
version: 1
direction: direction/forward
top level: BBa_K404229
start: 673
end: 1933
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098660
display id: annotation2098660
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098660/range2098660/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098660/range2098660/1
type: Range
persistent identity: range2098660
display id: range2098660
version: 1
direction: direction/forward
top level: BBa_K404229
start: 673
end: 1867
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2098961
display id: annotation2098961
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098961/range2098961/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098961/range2098961/1
type: Range
persistent identity: range2098961
display id: range2098961
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3650
end: 3694
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2098993
display id: annotation2098993
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098993/range2098993/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098993/range2098993/1
type: Range
persistent identity: range2098993
display id: range2098993
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3695
end: 3703
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101847
display id: annotation2101847
version: 1
top level: BBa_K404229
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2101847/range2101847/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2101847/range2101847/1
type: Range
persistent identity: range2101847
display id: range2101847
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3635
end: 3637
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2098994
display id: annotation2098994
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098994/range2098994/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098994/range2098994/1
type: Range
persistent identity: range2098994
display id: range2098994
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3612
end: 3643
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098811
display id: annotation2098811
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098811/range2098811/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098811/range2098811/1
type: Range
persistent identity: range2098811
display id: range2098811
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2294
end: 4150
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2098995
display id: annotation2098995
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098995/range2098995/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098995/range2098995/1
type: Range
persistent identity: range2098995
display id: range2098995
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3704
end: 3719
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098552
display id: annotation2098552
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098552/range2098552/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098552/range2098552/1
type: Range
persistent identity: range2098552
display id: range2098552
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2
end: 1867
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098608
display id: annotation2098608
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098608/range2098608/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098608/range2098608/1
type: Range
persistent identity: range2098608
display id: range2098608
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2
end: 1933
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2098988
display id: annotation2098988
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098988/range2098988/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098988/range2098988/1
type: Range
persistent identity: range2098988
display id: range2098988
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3644
end: 3649
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098773
display id: annotation2098773
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098773/range2098773/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098773/range2098773/1
type: Range
persistent identity: range2098773
display id: range2098773
version: 1
direction: direction/forward
top level: BBa_K404229
start: 1883
end: 4150
orientation: inline

BBa_K404007
type: ComponentDefinition
persistent identity: BBa K404007
display id: BBa_K404007
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP123
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: BBa_I712004
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP3
sequence annotation: VP1
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404007_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2099431
display id: annotation2099431
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099431/range2099431/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099431/range2099431/1
type: Range
persistent identity: range2099431
display id: range2099431
version: 1
direction: direction/forward
top level: BBa_K404007
start: 1079
end: 2875
orientation: inline

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation2099428
display id: annotation2099428
version: 1
top level: BBa_K404007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099428/range2099428/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099428/range2099428/1
type: Range
persistent identity: range2099428
display id: range2099428
version: 1
direction: direction/forward
top level: BBa_K404007
start: 2
end: 655
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2099430
display id: annotation2099430
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099430/range2099430/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099430/range2099430/1
type: Range
persistent identity: range2099430
display id: range2099430
version: 1
direction: direction/forward
top level: BBa_K404007
start: 671
end: 2998
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2099432
display id: annotation2099432
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099432/range2099432/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099432/range2099432/1
type: Range
persistent identity: range2099432
display id: range2099432
version: 1
direction: direction/forward
top level: BBa_K404007
start: 1274
end: 2875
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2099429
display id: annotation2099429
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099429/range2099429/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099429/range2099429/1
type: Range
persistent identity: range2099429
display id: range2099429
version: 1
direction: direction/forward
top level: BBa_K404007
start: 671
end: 2875
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2099427
display id: annotation2099427
version: 1
top level: BBa_K404007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099427/range2099427/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099427/range2099427/1
type: Range
persistent identity: range2099427
display id: range2099427
version: 1
direction: direction/forward
top level: BBa_K404007
start: 2
end: 655
orientation: inline

BBa_K404204
type: ComponentDefinition
persistent identity: BBa K404204
display id: BBa_K404204
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-453-Z34C
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstream 453
sequence annotation: downstream 453
sequence annotation: Z34C
sequence: https://synbiohub.org/public/igem/BBa_K404204_sequence/1

upstream 453
type: SequenceAnnotation
persistent identity: annotation2078944
display id: annotation2078944
version: 1
top level: BBa_K404204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404204/annotation2078944/range2078944/1

https://synbiohub.org/public/igem/BBa_K404204/annotation2078944/range2078944/1
type: Range
persistent identity: range2078944
display id: range2078944
version: 1
direction: direction/forward
top level: BBa_K404204
start: 1
end: 42
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2078946
display id: annotation2078946
version: 1
top level: BBa_K404204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404204/annotation2078946/range2078946/1

https://synbiohub.org/public/igem/BBa_K404204/annotation2078946/range2078946/1
type: Range
persistent identity: range2078946
display id: range2078946
version: 1
direction: direction/forward
top level: BBa_K404204
start: 145
end: 165
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2078945
display id: annotation2078945
version: 1
top level: BBa_K404204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404204/annotation2078945/range2078945/1

https://synbiohub.org/public/igem/BBa_K404204/annotation2078945/range2078945/1
type: Range
persistent identity: range2078945
display id: range2078945
version: 1
direction: direction/forward
top level: BBa_K404204
start: 43
end: 144
orientation: inline

BBa_K404224
type: ComponentDefinition
persistent identity: BBa K404224
display id: BBa_K404224
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-453-Z34C)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2 cap (Stratagene)
sequence annotation: p5 TATAless
sequence annotation: Rep 68
sequence annotation: Rep 78
sequence annotation: VP1
sequence annotation: Z34C
sequence annotation: Rep 52
sequence annotation: VP3
sequence annotation: Rep 40
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404224_sequence/1

AAV-2 cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2098661
display id: annotation2098661
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098661/range2098661/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098661/range2098661/1
type: Range
persistent identity: range2098661
display id: range2098661
version: 1
direction: direction/forward
top level: BBa_K404224
start: 1883
end: 3795
orientation: inline

p5 TATAless
type: SequenceAnnotation
persistent identity: annotation2098845
display id: annotation2098845
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098845/range2098845/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098845/range2098845/1
type: Range
persistent identity: range2098845
display id: range2098845
version: 1
direction: direction/forward
top level: BBa_K404224
start: 4324
end: 4487
orientation: inline

Rep 68
type: SequenceAnnotation
persistent identity: annotation2098424
display id: annotation2098424
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098424/range2098424/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098424/range2098424/1
type: Range
persistent identity: range2098424
display id: range2098424
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2
end: 1933
orientation: inline

Rep 78
type: SequenceAnnotation
persistent identity: annotation2098422
display id: annotation2098422
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098422/range2098422/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098422/range2098422/1
type: Range
persistent identity: range2098422
display id: range2098422
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2
end: 1867
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098551
display id: annotation2098551
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098551/range2098551/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098551/range2098551/1
type: Range
persistent identity: range2098551
display id: range2098551
version: 1
direction: direction/forward
top level: BBa_K404224
start: 1883
end: 4192
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2098940
display id: annotation2098940
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098940/range2098940/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098940/range2098940/1
type: Range
persistent identity: range2098940
display id: range2098940
version: 1
direction: direction/forward
top level: BBa_K404224
start: 3242
end: 3343
orientation: inline

Rep 52
type: SequenceAnnotation
persistent identity: annotation2098427
display id: annotation2098427
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098427/range2098427/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098427/range2098427/1
type: Range
persistent identity: range2098427
display id: range2098427
version: 1
direction: direction/forward
top level: BBa_K404224
start: 673
end: 1867
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098524
display id: annotation2098524
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098524/range2098524/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098524/range2098524/1
type: Range
persistent identity: range2098524
display id: range2098524
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2489
end: 4192
orientation: inline

Rep 40
type: SequenceAnnotation
persistent identity: annotation2098428
display id: annotation2098428
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098428/range2098428/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098428/range2098428/1
type: Range
persistent identity: range2098428
display id: range2098428
version: 1
direction: direction/forward
top level: BBa_K404224
start: 673
end: 1933
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098458
display id: annotation2098458
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098458/range2098458/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098458/range2098458/1
type: Range
persistent identity: range2098458
display id: range2098458
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2294
end: 4192
orientation: inline

BBa_K404151
type: ComponentDefinition
persistent identity: BBa K404151
display id: BBa_K404151
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404151_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2095299
display id: annotation2095299
version: 1
top level: BBa_K404151
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2095299/range2095299/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2095299/range2095299/1
type: Range
persistent identity: range2095299
display id: range2095299
version: 1
direction: direction/forward
top level: BBa_K404151
start: 193
end: 1794
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095300
display id: annotation2095300
version: 1
top level: BBa_K404151
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2095300/range2095300/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2095300/range2095300/1
type: Range
persistent identity: range2095300
display id: range2095300
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1
end: 1917
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101715
display id: annotation2101715
version: 1
top level: BBa_K404151
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2101715/range2101715/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2101715/range2101715/1
type: Range
persistent identity: range2101715
display id: range2101715
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1348
end: 1350
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095298
display id: annotation2095298
version: 1
top level: BBa_K404151
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2095298/range2095298/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2095298/range2095298/1
type: Range
persistent identity: range2095298
display id: range2095298
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1
end: 1794
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101716
display id: annotation2101716
version: 1
top level: BBa_K404151
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2101716/range2101716/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2101716/range2101716/1
type: Range
persistent identity: range2101716
display id: range2101716
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1339
end: 1341
orientation: inline

BBa_K404125
type: ComponentDefinition
persistent identity: BBa K404125
display id: BBa_K404125
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_phTERT_betaglobin_CD_hGH_[AAV2]-right-ITR
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
mutable provenance: x
owned by: james
owned by: myers
mutable description: x
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: x
star: false
creator: Freiburg Bioware 2010
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Beta-Globin-intron
sequence annotation: right ITR
sequence annotation: cytosine deaminase
sequence annotation: phTERT
sequence annotation: hGH terminator
sequence annotation: left ITR
sequence: https://synbiohub.org/public/igem/BBa_K404125_sequence/1

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093284
display id: annotation2093284
version: 1
top level: BBa_K404125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093284/range2093284/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093284/range2093284/1
type: Range
persistent identity: range2093284
display id: range2093284
version: 1
top level: BBa_K404125
direction: direction/forward
start: 619
end: 1113
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093287
display id: annotation2093287
version: 1
top level: BBa_K404125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093287/range2093287/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093287/range2093287/1
type: Range
persistent identity: range2093287
display id: range2093287
version: 1
top level: BBa_K404125
direction: direction/forward
start: 2919
end: 3056
orientation: inline

cytosine deaminase
type: SequenceAnnotation
persistent identity: annotation2093285
display id: annotation2093285
version: 1
top level: BBa_K404125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093285/range2093285/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093285/range2093285/1
type: Range
persistent identity: range2093285
display id: range2093285
version: 1
top level: BBa_K404125
direction: direction/forward
start: 1128
end: 2408
orientation: inline

phTERT
type: SequenceAnnotation
persistent identity: annotation2093282
display id: annotation2093282
version: 1
top level: BBa_K404125
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093282/range2093282/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093282/range2093282/1
type: Range
persistent identity: range2093282
display id: range2093282
version: 1
top level: BBa_K404125
direction: direction/forward
start: 154
end: 612
orientation: inline

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2093289
display id: annotation2093289
version: 1
top level: BBa_K404125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093289/range2093289/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093289/range2093289/1
type: Range
persistent identity: range2093289
display id: range2093289
version: 1
top level: BBa_K404125
direction: direction/forward
start: 2425
end: 2904
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093281
display id: annotation2093281
version: 1
top level: BBa_K404125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093281/range2093281/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093281/range2093281/1
type: Range
persistent identity: range2093281
display id: range2093281
version: 1
top level: BBa_K404125
direction: direction/forward
start: 1
end: 147
orientation: inline

BBa_K404126
type: ComponentDefinition
persistent identity: BBa K404126
display id: BBa_K404126
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_pCMV_betaglobin_CD_hGH_[AAV2]-right-ITR
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
mutable provenance: x
owned by: james
owned by: myers
mutable description: x
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: x
star: false
creator: Freiburg Bioware 2010
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Cytosine deaminase
sequence annotation: left ITR
sequence annotation: hGH
sequence annotation: CMV promoter
sequence annotation: Beta-Globin-intron
sequence annotation: right ITR
sequence: https://synbiohub.org/public/igem/BBa_K404126_sequence/1

Cytosine deaminase
type: SequenceAnnotation
persistent identity: annotation2093294
display id: annotation2093294
version: 1
top level: BBa_K404126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093294/range2093294/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093294/range2093294/1
type: Range
persistent identity: range2093294
display id: range2093294
version: 1
top level: BBa_K404126
direction: direction/forward
start: 1325
end: 2605
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093290
display id: annotation2093290
version: 1
top level: BBa_K404126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093290/range2093290/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093290/range2093290/1
type: Range
persistent identity: range2093290
display id: range2093290
version: 1
top level: BBa_K404126
direction: direction/forward
start: 1
end: 147
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2093297
display id: annotation2093297
version: 1
top level: BBa_K404126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093297/range2093297/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093297/range2093297/1
type: Range
persistent identity: range2093297
display id: range2093297
version: 1
top level: BBa_K404126
direction: direction/forward
start: 2623
end: 3101
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093292
display id: annotation2093292
version: 1
top level: BBa_K404126
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093292/range2093292/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093292/range2093292/1
type: Range
persistent identity: range2093292
display id: range2093292
version: 1
top level: BBa_K404126
direction: direction/forward
start: 155
end: 809
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093293
display id: annotation2093293
version: 1
top level: BBa_K404126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093293/range2093293/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093293/range2093293/1
type: Range
persistent identity: range2093293
display id: range2093293
version: 1
top level: BBa_K404126
direction: direction/forward
start: 816
end: 1310
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093296
display id: annotation2093296
version: 1
top level: BBa_K404126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093296/range2093296/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093296/range2093296/1
type: Range
persistent identity: range2093296
display id: range2093296
version: 1
top level: BBa_K404126
direction: direction/forward
start: 3116
end: 3253
orientation: inline

BBa_K404117
type: ComponentDefinition
persistent identity: BBa K404117
display id: BBa_K404117
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_pCMV_betaglobin
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: left ITR
sequence annotation: Beta-Globin-intron
sequence annotation: CMV promoter
sequence: https://synbiohub.org/public/igem/BBa_K404117_sequence/1

left ITR
type: SequenceAnnotation
persistent identity: annotation2093230
display id: annotation2093230
version: 1
top level: BBa_K404117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404117/annotation2093230/range2093230/1

https://synbiohub.org/public/igem/BBa_K404117/annotation2093230/range2093230/1
type: Range
persistent identity: range2093230
display id: range2093230
version: 1
direction: direction/forward
top level: BBa_K404117
start: 1
end: 147
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093232
display id: annotation2093232
version: 1
top level: BBa_K404117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404117/annotation2093232/range2093232/1

https://synbiohub.org/public/igem/BBa_K404117/annotation2093232/range2093232/1
type: Range
persistent identity: range2093232
display id: range2093232
version: 1
direction: direction/forward
top level: BBa_K404117
start: 816
end: 1310
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093231
display id: annotation2093231
version: 1
top level: BBa_K404117
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404117/annotation2093231/range2093231/1

https://synbiohub.org/public/igem/BBa_K404117/annotation2093231/range2093231/1
type: Range
persistent identity: range2093231
display id: range2093231
version: 1
direction: direction/forward
top level: BBa_K404117
start: 155
end: 809
orientation: inline

BBa_K404253
type: ComponentDefinition
persistent identity: BBa K404253
display id: BBa_K404253
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: His-tag
sequence annotation: VP1
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: GLS-spacer
sequence annotation: upstream 587
sequence annotation: VP3
sequence annotation: TG-spacer
sequence annotation: CMV
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404253_sequence/1

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101880
display id: annotation2101880
version: 1
top level: BBa_K404253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2101880/range2101880/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2101880/range2101880/1
type: Range
persistent identity: range2101880
display id: range2101880
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2420
end: 2422
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094655
display id: annotation2094655
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094655/range2094655/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094655/range2094655/1
type: Range
persistent identity: range2094655
display id: range2094655
version: 1
direction: direction/forward
top level: BBa_K404253
start: 1079
end: 2908
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2094658
display id: annotation2094658
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094658/range2094658/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094658/range2094658/1
type: Range
persistent identity: range2094658
display id: range2094658
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2435
end: 2452
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094654
display id: annotation2094654
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094654/range2094654/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094654/range2094654/1
type: Range
persistent identity: range2094654
display id: range2094654
version: 1
direction: direction/forward
top level: BBa_K404253
start: 671
end: 2908
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101879
display id: annotation2101879
version: 1
top level: BBa_K404253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2101879/range2101879/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2101879/range2101879/1
type: Range
persistent identity: range2101879
display id: range2101879
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2454
end: 2456
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094657
display id: annotation2094657
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094657/range2094657/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094657/range2094657/1
type: Range
persistent identity: range2094657
display id: range2094657
version: 1
direction: direction/forward
top level: BBa_K404253
start: 671
end: 3031
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094660
display id: annotation2094660
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094660/range2094660/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094660/range2094660/1
type: Range
persistent identity: range2094660
display id: range2094660
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2453
end: 2461
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094661
display id: annotation2094661
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094661/range2094661/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094661/range2094661/1
type: Range
persistent identity: range2094661
display id: range2094661
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2397
end: 2428
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094656
display id: annotation2094656
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094656/range2094656/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094656/range2094656/1
type: Range
persistent identity: range2094656
display id: range2094656
version: 1
direction: direction/forward
top level: BBa_K404253
start: 1274
end: 2908
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094659
display id: annotation2094659
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094659/range2094659/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094659/range2094659/1
type: Range
persistent identity: range2094659
display id: range2094659
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2429
end: 2434
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094653
display id: annotation2094653
version: 1
top level: BBa_K404253
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094653/range2094653/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094653/range2094653/1
type: Range
persistent identity: range2094653
display id: range2094653
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2
end: 655
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094662
display id: annotation2094662
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094662/range2094662/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094662/range2094662/1
type: Range
persistent identity: range2094662
display id: range2094662
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2462
end: 2477
orientation: inline

BBa_K404243
type: ComponentDefinition
persistent identity: BBa K404243
display id: BBa_K404243
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]VP123 (ViralBrick-453-RGD)
creator: Freiburg Bioware 2010
created: 2010-09-19T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: RGD
sequence annotation: VP3
sequence annotation: A-spacer
sequence annotation: VP2
sequence annotation: downstream 453
sequence annotation: VP1
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: upstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404243_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2094244
display id: annotation2094244
version: 1
top level: BBa_K404243
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094244/range2094244/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094244/range2094244/1
type: Range
persistent identity: range2094244
display id: range2094244
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2
end: 655
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2094251
display id: annotation2094251
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094251/range2094251/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094251/range2094251/1
type: Range
persistent identity: range2094251
display id: range2094251
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2030
end: 2056
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094247
display id: annotation2094247
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094247/range2094247/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094247/range2094247/1
type: Range
persistent identity: range2094247
display id: range2094247
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1274
end: 2908
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2094250
display id: annotation2094250
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094250/range2094250/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094250/range2094250/1
type: Range
persistent identity: range2094250
display id: range2094250
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2027
end: 2029
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2094253
display id: annotation2094253
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094253/range2094253/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094253/range2094253/1
type: Range
persistent identity: range2094253
display id: range2094253
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2057
end: 2059
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094246
display id: annotation2094246
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094246/range2094246/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094246/range2094246/1
type: Range
persistent identity: range2094246
display id: range2094246
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1079
end: 2908
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2094252
display id: annotation2094252
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094252/range2094252/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094252/range2094252/1
type: Range
persistent identity: range2094252
display id: range2094252
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2060
end: 2073
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094245
display id: annotation2094245
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094245/range2094245/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094245/range2094245/1
type: Range
persistent identity: range2094245
display id: range2094245
version: 1
direction: direction/forward
top level: BBa_K404243
start: 671
end: 2908
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094248
display id: annotation2094248
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094248/range2094248/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094248/range2094248/1
type: Range
persistent identity: range2094248
display id: range2094248
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1720
end: 3031
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2094249
display id: annotation2094249
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094249/range2094249/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094249/range2094249/1
type: Range
persistent identity: range2094249
display id: range2094249
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1989
end: 2026
orientation: inline

BBa_K404307
type: ComponentDefinition
persistent identity: BBa K404307
display id: BBa_K404307
version: 1
was generated by: iGEM to SBOL conversion
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite

BBa_K404163
type: ComponentDefinition
persistent identity: BBa K404163
display id: BBa_K404163
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: TG-spacer
sequence annotation: downstream 587
sequence annotation: A20-2 binding site
sequence annotation: basic region 2
sequence annotation: scar
sequence annotation: B1 binding site
sequence annotation: GLS-spacer
sequence annotation: middle-linker
sequence annotation: A20-4 binding site
sequence annotation: upstream 587
sequence annotation: basic region 3
sequence annotation: VP3
sequence annotation: basic region 4
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: ZEGFR_1907
sequence annotation: His-tag
sequence annotation: CMV
sequence annotation: A20-1 binding site
sequence: https://synbiohub.org/public/igem/BBa_K404163_sequence/1

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2101705
display id: annotation2101705
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101705/range2101705/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101705/range2101705/1
type: Range
persistent identity: range2101705
display id: range2101705
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2228
end: 2233
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2101708
display id: annotation2101708
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101708/range2101708/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101708/range2101708/1
type: Range
persistent identity: range2101708
display id: range2101708
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2261
end: 2276
orientation: inline

A20-2 binding site
type: SequenceAnnotation
persistent identity: annotation2101701
display id: annotation2101701
version: 1
top level: BBa_K404163
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101701/range2101701/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101701/range2101701/1
type: Range
persistent identity: range2101701
display id: range2101701
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1574
end: 1600
orientation: inline

basic region 2
type: SequenceAnnotation
persistent identity: annotation2101696
display id: annotation2101696
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101696/range2101696/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101696/range2101696/1
type: Range
persistent identity: range2101696
display id: range2101696
version: 1
direction: direction/forward
top level: BBa_K404163
start: 884
end: 904
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101693
display id: annotation2101693
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101693/range2101693/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101693/range2101693/1
type: Range
persistent identity: range2101693
display id: range2101693
version: 1
direction: direction/forward
top level: BBa_K404163
start: 875
end: 880
orientation: inline

B1 binding site
type: SequenceAnnotation
persistent identity: annotation2101710
display id: annotation2101710
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101710/range2101710/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101710/range2101710/1
type: Range
persistent identity: range2101710
display id: range2101710
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2675
end: 2698
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101691
display id: annotation2101691
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101691/range2101691/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101691/range2101691/1
type: Range
persistent identity: range2101691
display id: range2101691
version: 1
direction: direction/forward
top level: BBa_K404163
start: 845
end: 850
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2101707
display id: annotation2101707
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101707/range2101707/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101707/range2101707/1
type: Range
persistent identity: range2101707
display id: range2101707
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2252
end: 2260
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2101692
display id: annotation2101692
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101692/range2101692/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101692/range2101692/1
type: Range
persistent identity: range2101692
display id: range2101692
version: 1
direction: direction/forward
top level: BBa_K404163
start: 851
end: 874
orientation: inline

A20-4 binding site
type: SequenceAnnotation
persistent identity: annotation2101702
display id: annotation2101702
version: 1
top level: BBa_K404163
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101702/range2101702/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101702/range2101702/1
type: Range
persistent identity: range2101702
display id: range2101702
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2162
end: 2191
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2101703
display id: annotation2101703
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101703/range2101703/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101703/range2101703/1
type: Range
persistent identity: range2101703
display id: range2101703
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2196
end: 2227
orientation: inline

basic region 3
type: SequenceAnnotation
persistent identity: annotation2101697
display id: annotation2101697
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101697/range2101697/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101697/range2101697/1
type: Range
persistent identity: range2101697
display id: range2101697
version: 1
direction: direction/forward
top level: BBa_K404163
start: 962
end: 982
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2101698
display id: annotation2101698
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101698/range2101698/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101698/range2101698/1
type: Range
persistent identity: range2101698
display id: range2101698
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1073
end: 2707
orientation: inline

basic region 4
type: SequenceAnnotation
persistent identity: annotation2101700
display id: annotation2101700
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101700/range2101700/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101700/range2101700/1
type: Range
persistent identity: range2101700
display id: range2101700
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1385
end: 1402
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101704
display id: annotation2101704
version: 1
top level: BBa_K404163
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101704/range2101704/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101704/range2101704/1
type: Range
persistent identity: range2101704
display id: range2101704
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2219
end: 2221
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2101694
display id: annotation2101694
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101694/range2101694/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101694/range2101694/1
type: Range
persistent identity: range2101694
display id: range2101694
version: 1
direction: direction/forward
top level: BBa_K404163
start: 881
end: 2707
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101709
display id: annotation2101709
version: 1
top level: BBa_K404163
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101709/range2101709/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101709/range2101709/1
type: Range
persistent identity: range2101709
display id: range2101709
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2288
end: 2290
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2101695
display id: annotation2101695
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101695/range2101695/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101695/range2101695/1
type: Range
persistent identity: range2101695
display id: range2101695
version: 1
direction: direction/forward
top level: BBa_K404163
start: 881
end: 2830
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2101690
display id: annotation2101690
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101690/range2101690/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101690/range2101690/1
type: Range
persistent identity: range2101690
display id: range2101690
version: 1
direction: direction/forward
top level: BBa_K404163
start: 671
end: 844
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2101706
display id: annotation2101706
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101706/range2101706/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101706/range2101706/1
type: Range
persistent identity: range2101706
display id: range2101706
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2234
end: 2251
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2101688
display id: annotation2101688
version: 1
top level: BBa_K404163
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101688/range2101688/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101688/range2101688/1
type: Range
persistent identity: range2101688
display id: range2101688
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2
end: 655
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101689
display id: annotation2101689
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101689/range2101689/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101689/range2101689/1
type: Range
persistent identity: range2101689
display id: range2101689
version: 1
direction: direction/forward
top level: BBa_K404163
start: 656
end: 670
orientation: inline

A20-1 binding site
type: SequenceAnnotation
persistent identity: annotation2101699
display id: annotation2101699
version: 1
top level: BBa_K404163
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101699/range2101699/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101699/range2101699/1
type: Range
persistent identity: range2101699
display id: range2101699
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1277
end: 1306
orientation: inline

BBa_K404102
type: ComponentDefinition
persistent identity: BBa K404102
display id: BBa_K404102
version: 1
was generated by: iGEM to SBOL conversion
description: CMV promoter
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CMV promoter
sequence: https://synbiohub.org/public/igem/BBa_K404102_sequence/1

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094574
display id: annotation2094574
version: 1
top level: BBa_K404102
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404102/annotation2094574/range2094574/1

https://synbiohub.org/public/igem/BBa_K404102/annotation2094574/range2094574/1
type: Range
persistent identity: range2094574
display id: range2094574
version: 1
direction: direction/forward
top level: BBa_K404102
start: 2
end: 655
orientation: inline

BBa_K404152
type: ComponentDefinition
persistent identity: BBa K404152
display id: BBa_K404152
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-VP1up
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1up
sequence: https://synbiohub.org/public/igem/BBa_K404152_sequence/1

VP1up
type: SequenceAnnotation
persistent identity: annotation2093343
display id: annotation2093343
version: 1
top level: BBa_K404152
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404152/annotation2093343/range2093343/1

https://synbiohub.org/public/igem/BBa_K404152/annotation2093343/range2093343/1
type: Range
persistent identity: range2093343
display id: range2093343
version: 1
direction: direction/forward
top level: BBa_K404152
start: 1
end: 411
orientation: inline

BBa_K404156
type: ComponentDefinition
persistent identity: BBa K404156
display id: BBa_K404156
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: HSPG-KO
sequence annotation: ZEGFR_1907
sequence annotation: CMV
sequence annotation: middle-linker
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404156_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2094980
display id: annotation2094980
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094980/range2094980/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094980/range2094980/1
type: Range
persistent identity: range2094980
display id: range2094980
version: 1
direction: direction/forward
top level: BBa_K404156
start: 881
end: 2674
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101755
display id: annotation2101755
version: 1
top level: BBa_K404156
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2101755/range2101755/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2101755/range2101755/1
type: Range
persistent identity: range2101755
display id: range2101755
version: 1
direction: direction/forward
top level: BBa_K404156
start: 2228
end: 2230
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2094993
display id: annotation2094993
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094993/range2094993/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094993/range2094993/1
type: Range
persistent identity: range2094993
display id: range2094993
version: 1
direction: direction/forward
top level: BBa_K404156
start: 671
end: 844
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094979
display id: annotation2094979
version: 1
top level: BBa_K404156
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094979/range2094979/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094979/range2094979/1
type: Range
persistent identity: range2094979
display id: range2094979
version: 1
direction: direction/forward
top level: BBa_K404156
start: 2
end: 655
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2094992
display id: annotation2094992
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094992/range2094992/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094992/range2094992/1
type: Range
persistent identity: range2094992
display id: range2094992
version: 1
direction: direction/forward
top level: BBa_K404156
start: 851
end: 874
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094991
display id: annotation2094991
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094991/range2094991/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094991/range2094991/1
type: Range
persistent identity: range2094991
display id: range2094991
version: 1
direction: direction/forward
top level: BBa_K404156
start: 881
end: 2797
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094981
display id: annotation2094981
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094981/range2094981/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094981/range2094981/1
type: Range
persistent identity: range2094981
display id: range2094981
version: 1
direction: direction/forward
top level: BBa_K404156
start: 1073
end: 2674
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101782
display id: annotation2101782
version: 1
top level: BBa_K404156
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2101782/range2101782/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2101782/range2101782/1
type: Range
persistent identity: range2101782
display id: range2101782
version: 1
direction: direction/forward
top level: BBa_K404156
start: 2219
end: 2221
orientation: inline

BBa_K404233
type: ComponentDefinition
persistent identity: BBa K404233
display id: BBa_K404233
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-587KO-Z34C)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404248
type: ComponentDefinition
persistent identity: BBa K404248
display id: BBa_K404248
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-RGD)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: RGD
sequence annotation: VP1
sequence annotation: CMV promoter
sequence annotation: TG-spacer
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: upstream 587
sequence annotation: downstream 587
sequence annotation: GLS-spacer
sequence: https://synbiohub.org/public/igem/BBa_K404248_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2094314
display id: annotation2094314
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094314/range2094314/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094314/range2094314/1
type: Range
persistent identity: range2094314
display id: range2094314
version: 1
direction: direction/forward
top level: BBa_K404248
start: 1079
end: 2917
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094315
display id: annotation2094315
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094315/range2094315/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094315/range2094315/1
type: Range
persistent identity: range2094315
display id: range2094315
version: 1
direction: direction/forward
top level: BBa_K404248
start: 1274
end: 2917
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2094371
display id: annotation2094371
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094371/range2094371/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094371/range2094371/1
type: Range
persistent identity: range2094371
display id: range2094371
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2435
end: 2461
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094295
display id: annotation2094295
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094295/range2094295/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094295/range2094295/1
type: Range
persistent identity: range2094295
display id: range2094295
version: 1
direction: direction/forward
top level: BBa_K404248
start: 671
end: 2917
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094294
display id: annotation2094294
version: 1
top level: BBa_K404248
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094294/range2094294/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094294/range2094294/1
type: Range
persistent identity: range2094294
display id: range2094294
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2
end: 655
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094410
display id: annotation2094410
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094410/range2094410/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094410/range2094410/1
type: Range
persistent identity: range2094410
display id: range2094410
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2429
end: 2434
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094316
display id: annotation2094316
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094316/range2094316/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094316/range2094316/1
type: Range
persistent identity: range2094316
display id: range2094316
version: 1
direction: direction/forward
top level: BBa_K404248
start: 671
end: 3040
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094390
display id: annotation2094390
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094390/range2094390/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094390/range2094390/1
type: Range
persistent identity: range2094390
display id: range2094390
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2397
end: 2428
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094391
display id: annotation2094391
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094391/range2094391/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094391/range2094391/1
type: Range
persistent identity: range2094391
display id: range2094391
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2471
end: 2486
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094429
display id: annotation2094429
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094429/range2094429/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094429/range2094429/1
type: Range
persistent identity: range2094429
display id: range2094429
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2462
end: 2470
orientation: inline

BBa_K404206
type: ComponentDefinition
persistent identity: BBa K404206
display id: BBa_K404206
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-587-His-Tag
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: GLS Spacer
sequence annotation: downstream 453
sequence annotation: His-Tag
sequence annotation: upstream 587
sequence annotation: upstream 453
sequence annotation: TG Spacer
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404206_sequence/1

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2078958
display id: annotation2078958
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078958/range2078958/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078958/range2078958/1
type: Range
persistent identity: range2078958
display id: range2078958
version: 1
direction: direction/forward
top level: BBa_K404206
start: 67
end: 75
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2078960
display id: annotation2078960
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078960/range2078960/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078960/range2078960/1
type: Range
persistent identity: range2078960
display id: range2078960
version: 1
direction: direction/forward
top level: BBa_K404206
start: 97
end: 105
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2078957
display id: annotation2078957
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078957/range2078957/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078957/range2078957/1
type: Range
persistent identity: range2078957
display id: range2078957
version: 1
direction: direction/forward
top level: BBa_K404206
start: 49
end: 66
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2078955
display id: annotation2078955
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078955/range2078955/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078955/range2078955/1
type: Range
persistent identity: range2078955
display id: range2078955
version: 1
direction: direction/forward
top level: BBa_K404206
start: 10
end: 42
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2078954
display id: annotation2078954
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078954/range2078954/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078954/range2078954/1
type: Range
persistent identity: range2078954
display id: range2078954
version: 1
direction: direction/forward
top level: BBa_K404206
start: 1
end: 9
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2078956
display id: annotation2078956
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078956/range2078956/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078956/range2078956/1
type: Range
persistent identity: range2078956
display id: range2078956
version: 1
direction: direction/forward
top level: BBa_K404206
start: 43
end: 48
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2078959
display id: annotation2078959
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078959/range2078959/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078959/range2078959/1
type: Range
persistent identity: range2078959
display id: range2078959
version: 1
direction: direction/forward
top level: BBa_K404206
start: 75
end: 96
orientation: inline

BBa_K404101
type: ComponentDefinition
persistent identity: BBa K404101
display id: BBa_K404101
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: right ITR
sequence: https://synbiohub.org/public/igem/BBa_K404101_sequence/1

right ITR
type: SequenceAnnotation
persistent identity: annotation2093167
display id: annotation2093167
version: 1
top level: BBa_K404101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404101/annotation2093167/range2093167/1

https://synbiohub.org/public/igem/BBa_K404101/annotation2093167/range2093167/1
type: Range
persistent identity: range2093167
display id: range2093167
version: 1
direction: direction/forward
top level: BBa_K404101
start: 1
end: 138
orientation: inline

BBa_K404256
type: ComponentDefinition
persistent identity: BBa K404256
display id: BBa_K404256
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Z34C-Spacer)
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: downstream 587
sequence annotation: upstream 587
sequence annotation: TG-spacer
sequence annotation: AAV--1-cap (Stratagene)
sequence annotation: HSPG-KO
sequence annotation: CMV
sequence annotation: Z34C
sequence annotation: GLS-spacer
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404256_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2094727
display id: annotation2094727
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094727/range2094727/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094727/range2094727/1
type: Range
persistent identity: range2094727
display id: range2094727
version: 1
direction: direction/forward
top level: BBa_K404256
start: 671
end: 2992
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094729
display id: annotation2094729
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094729/range2094729/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094729/range2094729/1
type: Range
persistent identity: range2094729
display id: range2094729
version: 1
direction: direction/forward
top level: BBa_K404256
start: 1274
end: 2992
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094735
display id: annotation2094735
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094735/range2094735/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094735/range2094735/1
type: Range
persistent identity: range2094735
display id: range2094735
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2546
end: 2561
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094736
display id: annotation2094736
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094736/range2094736/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094736/range2094736/1
type: Range
persistent identity: range2094736
display id: range2094736
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2397
end: 2428
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094733
display id: annotation2094733
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094733/range2094733/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094733/range2094733/1
type: Range
persistent identity: range2094733
display id: range2094733
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2429
end: 2434
orientation: inline

AAV--1-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094730
display id: annotation2094730
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094730/range2094730/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094730/range2094730/1
type: Range
persistent identity: range2094730
display id: range2094730
version: 1
direction: direction/forward
top level: BBa_K404256
start: 671
end: 3115
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101894
display id: annotation2101894
version: 1
top level: BBa_K404256
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2101894/range2101894/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2101894/range2101894/1
type: Range
persistent identity: range2101894
display id: range2101894
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2546
end: 2548
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101929
display id: annotation2101929
version: 1
top level: BBa_K404256
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2101929/range2101929/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2101929/range2101929/1
type: Range
persistent identity: range2101929
display id: range2101929
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2433
end: 2435
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094726
display id: annotation2094726
version: 1
top level: BBa_K404256
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094726/range2094726/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094726/range2094726/1
type: Range
persistent identity: range2094726
display id: range2094726
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2
end: 655
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2094731
display id: annotation2094731
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094731/range2094731/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094731/range2094731/1
type: Range
persistent identity: range2094731
display id: range2094731
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2435
end: 2536
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094734
display id: annotation2094734
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094734/range2094734/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094734/range2094734/1
type: Range
persistent identity: range2094734
display id: range2094734
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2537
end: 2545
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094728
display id: annotation2094728
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094728/range2094728/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094728/range2094728/1
type: Range
persistent identity: range2094728
display id: range2094728
version: 1
direction: direction/forward
top level: BBa_K404256
start: 1079
end: 2992
orientation: inline

BBa_K404123
type: ComponentDefinition
persistent identity: BBa K404123
display id: BBa_K404123
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_phTERT_betaglobin_mVenus_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: phTERT promoter
sequence annotation: Beta-Globin-intron
sequence annotation: hGH
sequence annotation: mVenus
sequence annotation: left ITR
sequence annotation: right ITR
sequence: https://synbiohub.org/public/igem/BBa_K404123_sequence/1

phTERT promoter
type: SequenceAnnotation
persistent identity: annotation2093276
display id: annotation2093276
version: 1
top level: BBa_K404123
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093276/range2093276/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093276/range2093276/1
type: Range
persistent identity: range2093276
display id: range2093276
version: 1
direction: direction/forward
top level: BBa_K404123
start: 154
end: 612
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093277
display id: annotation2093277
version: 1
top level: BBa_K404123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093277/range2093277/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093277/range2093277/1
type: Range
persistent identity: range2093277
display id: range2093277
version: 1
direction: direction/forward
top level: BBa_K404123
start: 619
end: 1113
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2093279
display id: annotation2093279
version: 1
top level: BBa_K404123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093279/range2093279/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093279/range2093279/1
type: Range
persistent identity: range2093279
display id: range2093279
version: 1
direction: direction/forward
top level: BBa_K404123
start: 1862
end: 2337
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2093278
display id: annotation2093278
version: 1
top level: BBa_K404123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093278/range2093278/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093278/range2093278/1
type: Range
persistent identity: range2093278
display id: range2093278
version: 1
direction: direction/forward
top level: BBa_K404123
start: 1128
end: 1841
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093275
display id: annotation2093275
version: 1
top level: BBa_K404123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093275/range2093275/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093275/range2093275/1
type: Range
persistent identity: range2093275
display id: range2093275
version: 1
direction: direction/forward
top level: BBa_K404123
start: 1
end: 147
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093280
display id: annotation2093280
version: 1
top level: BBa_K404123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093280/range2093280/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093280/range2093280/1
type: Range
persistent identity: range2093280
display id: range2093280
version: 1
direction: direction/forward
top level: BBa_K404123
start: 2352
end: 2489
orientation: inline

BBa_K404251
type: ComponentDefinition
persistent identity: BBa K404251
display id: BBa_K404251
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-BAP)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence annotation: HSPG-KO
sequence annotation: upstream 587
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: downstream 587
sequence annotation: VP2
sequence annotation: CMV promoter
sequence annotation: VP3
sequence annotation: GLS-spacer
sequence annotation: TG-spacer
sequence annotation: BAP
sequence: https://synbiohub.org/public/igem/BBa_K404251_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2094522
display id: annotation2094522
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094522/range2094522/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094522/range2094522/1
type: Range
persistent identity: range2094522
display id: range2094522
version: 1
direction: direction/forward
top level: BBa_K404251
start: 671
end: 2935
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101875
display id: annotation2101875
version: 1
top level: BBa_K404251
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2101875/range2101875/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2101875/range2101875/1
type: Range
persistent identity: range2101875
display id: range2101875
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2481
end: 2483
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094526
display id: annotation2094526
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094526/range2094526/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094526/range2094526/1
type: Range
persistent identity: range2094526
display id: range2094526
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2397
end: 2428
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094523
display id: annotation2094523
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094523/range2094523/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094523/range2094523/1
type: Range
persistent identity: range2094523
display id: range2094523
version: 1
direction: direction/forward
top level: BBa_K404251
start: 671
end: 3058
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094530
display id: annotation2094530
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094530/range2094530/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094530/range2094530/1
type: Range
persistent identity: range2094530
display id: range2094530
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2489
end: 2504
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094524
display id: annotation2094524
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094524/range2094524/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094524/range2094524/1
type: Range
persistent identity: range2094524
display id: range2094524
version: 1
direction: direction/forward
top level: BBa_K404251
start: 1079
end: 2935
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094521
display id: annotation2094521
version: 1
top level: BBa_K404251
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094521/range2094521/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094521/range2094521/1
type: Range
persistent identity: range2094521
display id: range2094521
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094525
display id: annotation2094525
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094525/range2094525/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094525/range2094525/1
type: Range
persistent identity: range2094525
display id: range2094525
version: 1
direction: direction/forward
top level: BBa_K404251
start: 1274
end: 2935
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094529
display id: annotation2094529
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094529/range2094529/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094529/range2094529/1
type: Range
persistent identity: range2094529
display id: range2094529
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2480
end: 2488
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094527
display id: annotation2094527
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094527/range2094527/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094527/range2094527/1
type: Range
persistent identity: range2094527
display id: range2094527
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2429
end: 2434
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101876
display id: annotation2101876
version: 1
top level: BBa_K404251
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2101876/range2101876/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2101876/range2101876/1
type: Range
persistent identity: range2101876
display id: range2101876
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2419
end: 2421
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2094528
display id: annotation2094528
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094528/range2094528/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094528/range2094528/1
type: Range
persistent identity: range2094528
display id: range2094528
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2435
end: 2479
orientation: inline

BBa_K404166
type: ComponentDefinition
persistent identity: BBa K404166
display id: BBa_K404166
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_VP1up_NLS_His-Tag_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: NLS
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: VP1up
sequence annotation: CMV
sequence annotation: VP1
sequence annotation: His-tag
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404166_sequence/1

NLS
type: SequenceAnnotation
persistent identity: annotation2094645
display id: annotation2094645
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094645/range2094645/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094645/range2094645/1
type: Range
persistent identity: range2094645
display id: range2094645
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1089
end: 1109
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101830
display id: annotation2101830
version: 1
top level: BBa_K404166
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2101830/range2101830/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2101830/range2101830/1
type: Range
persistent identity: range2101830
display id: range2101830
version: 1
direction: direction/forward
top level: BBa_K404166
start: 2478
end: 2480
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094648
display id: annotation2094648
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094648/range2094648/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094648/range2094648/1
type: Range
persistent identity: range2094648
display id: range2094648
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1140
end: 2933
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094650
display id: annotation2094650
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094650/range2094650/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094650/range2094650/1
type: Range
persistent identity: range2094650
display id: range2094650
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1332
end: 2933
orientation: inline

VP1up
type: SequenceAnnotation
persistent identity: annotation2094644
display id: annotation2094644
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094644/range2094644/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094644/range2094644/1
type: Range
persistent identity: range2094644
display id: range2094644
version: 1
direction: direction/forward
top level: BBa_K404166
start: 674
end: 1082
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094643
display id: annotation2094643
version: 1
top level: BBa_K404166
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094643/range2094643/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094643/range2094643/1
type: Range
persistent identity: range2094643
display id: range2094643
version: 1
direction: direction/forward
top level: BBa_K404166
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101818
display id: annotation2101818
version: 1
top level: BBa_K404166
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2101818/range2101818/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2101818/range2101818/1
type: Range
persistent identity: range2101818
display id: range2101818
version: 1
direction: direction/forward
top level: BBa_K404166
start: 2487
end: 2489
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094649
display id: annotation2094649
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094649/range2094649/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094649/range2094649/1
type: Range
persistent identity: range2094649
display id: range2094649
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1140
end: 2933
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2094646
display id: annotation2094646
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094646/range2094646/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094646/range2094646/1
type: Range
persistent identity: range2094646
display id: range2094646
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1116
end: 1133
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094647
display id: annotation2094647
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094647/range2094647/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094647/range2094647/1
type: Range
persistent identity: range2094647
display id: range2094647
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1140
end: 3056
orientation: inline

BBa_K404116
type: ComponentDefinition
persistent identity: BBa K404116
display id: BBa_K404116
version: 1
was generated by: iGEM to SBOL conversion
description: hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: hGH
sequence annotation: right ITR
sequence: https://synbiohub.org/public/igem/BBa_K404116_sequence/1

hGH
type: SequenceAnnotation
persistent identity: annotation2093227
display id: annotation2093227
version: 1
top level: BBa_K404116
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404116/annotation2093227/range2093227/1

https://synbiohub.org/public/igem/BBa_K404116/annotation2093227/range2093227/1
type: Range
persistent identity: range2093227
display id: range2093227
version: 1
direction: direction/forward
top level: BBa_K404116
start: 1
end: 482
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093229
display id: annotation2093229
version: 1
top level: BBa_K404116
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404116/annotation2093229/range2093229/1

https://synbiohub.org/public/igem/BBa_K404116/annotation2093229/range2093229/1
type: Range
persistent identity: range2093229
display id: range2093229
version: 1
direction: direction/forward
top level: BBa_K404116
start: 495
end: 632
orientation: inline

BBa_K404210
type: ComponentDefinition
persistent identity: BBa K404210
display id: BBa_K404210
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-587KO-Empty
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstream 453
sequence annotation: downstream 587
sequence annotation: upstream 587
sequence annotation: R=>A
sequence annotation: downstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404210_sequence/1

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079125
display id: annotation2079125
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079125/range2079125/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079125/range2079125/1
type: Range
persistent identity: range2079125
display id: range2079125
version: 1
direction: direction/forward
top level: BBa_K404210
start: 1
end: 9
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079127
display id: annotation2079127
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079127/range2079127/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079127/range2079127/1
type: Range
persistent identity: range2079127
display id: range2079127
version: 1
direction: direction/forward
top level: BBa_K404210
start: 43
end: 63
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079126
display id: annotation2079126
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079126/range2079126/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079126/range2079126/1
type: Range
persistent identity: range2079126
display id: range2079126
version: 1
direction: direction/forward
top level: BBa_K404210
start: 10
end: 42
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079129
display id: annotation2079129
version: 1
top level: BBa_K404210
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079129/range2079129/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079129/range2079129/1
type: Range
persistent identity: range2079129
display id: range2079129
version: 1
direction: direction/forward
top level: BBa_K404210
start: 34
end: 36
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079128
display id: annotation2079128
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079128/range2079128/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079128/range2079128/1
type: Range
persistent identity: range2079128
display id: range2079128
version: 1
direction: direction/forward
top level: BBa_K404210
start: 64
end: 72
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079130
display id: annotation2079130
version: 1
top level: BBa_K404210
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079130/range2079130/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079130/range2079130/1
type: Range
persistent identity: range2079130
display id: range2079130
version: 1
direction: direction/forward
top level: BBa_K404210
start: 43
end: 45
orientation: inline

BBa_K404244
type: ComponentDefinition
persistent identity: BBa K404244
display id: BBa_K404244
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]VP123 (ViralBrick-453-Z34C)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Z34C
sequence annotation: VP1
sequence annotation: CMV promoter
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: AAV-2-cap (Strategene)
sequence: https://synbiohub.org/public/igem/BBa_K404244_sequence/1

Z34C
type: SequenceAnnotation
persistent identity: annotation2094259
display id: annotation2094259
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094259/range2094259/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094259/range2094259/1
type: Range
persistent identity: range2094259
display id: range2094259
version: 1
direction: direction/forward
top level: BBa_K404244
start: 2027
end: 2128
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094255
display id: annotation2094255
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094255/range2094255/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094255/range2094255/1
type: Range
persistent identity: range2094255
display id: range2094255
version: 1
direction: direction/forward
top level: BBa_K404244
start: 671
end: 2977
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094254
display id: annotation2094254
version: 1
top level: BBa_K404244
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094254/range2094254/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094254/range2094254/1
type: Range
persistent identity: range2094254
display id: range2094254
version: 1
direction: direction/forward
top level: BBa_K404244
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094257
display id: annotation2094257
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094257/range2094257/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094257/range2094257/1
type: Range
persistent identity: range2094257
display id: range2094257
version: 1
direction: direction/forward
top level: BBa_K404244
start: 1274
end: 2977
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094256
display id: annotation2094256
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094256/range2094256/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094256/range2094256/1
type: Range
persistent identity: range2094256
display id: range2094256
version: 1
direction: direction/forward
top level: BBa_K404244
start: 1079
end: 2977
orientation: inline

AAV-2-cap (Strategene)
type: SequenceAnnotation
persistent identity: annotation2094258
display id: annotation2094258
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094258/range2094258/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094258/range2094258/1
type: Range
persistent identity: range2094258
display id: range2094258
version: 1
direction: direction/forward
top level: BBa_K404244
start: 671
end: 3100
orientation: inline

BBa_K404250
type: ComponentDefinition
persistent identity: BBa K404250
display id: BBa_K404250
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-Beta-lactamase)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstream 587
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: downstream 587
sequence annotation: CMV promoter
sequence annotation: Beta-lactamase
sequence annotation: VP2
sequence annotation: VP1
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404250_sequence/1

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094508
display id: annotation2094508
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094508/range2094508/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094508/range2094508/1
type: Range
persistent identity: range2094508
display id: range2094508
version: 1
direction: direction/forward
top level: BBa_K404250
start: 2397
end: 2428
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094506
display id: annotation2094506
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094506/range2094506/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094506/range2094506/1
type: Range
persistent identity: range2094506
display id: range2094506
version: 1
direction: direction/forward
top level: BBa_K404250
start: 671
end: 3787
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094509
display id: annotation2094509
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094509/range2094509/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094509/range2094509/1
type: Range
persistent identity: range2094509
display id: range2094509
version: 1
direction: direction/forward
top level: BBa_K404250
start: 3218
end: 3233
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094448
display id: annotation2094448
version: 1
top level: BBa_K404250
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094448/range2094448/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094448/range2094448/1
type: Range
persistent identity: range2094448
display id: range2094448
version: 1
direction: direction/forward
top level: BBa_K404250
start: 2
end: 655
orientation: inline

Beta-lactamase
type: SequenceAnnotation
persistent identity: annotation2094507
display id: annotation2094507
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094507/range2094507/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094507/range2094507/1
type: Range
persistent identity: range2094507
display id: range2094507
version: 1
direction: direction/forward
top level: BBa_K404250
start: 2429
end: 3217
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094504
display id: annotation2094504
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094504/range2094504/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094504/range2094504/1
type: Range
persistent identity: range2094504
display id: range2094504
version: 1
direction: direction/forward
top level: BBa_K404250
start: 1079
end: 3664
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094449
display id: annotation2094449
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094449/range2094449/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094449/range2094449/1
type: Range
persistent identity: range2094449
display id: range2094449
version: 1
direction: direction/forward
top level: BBa_K404250
start: 671
end: 3664
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094505
display id: annotation2094505
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094505/range2094505/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094505/range2094505/1
type: Range
persistent identity: range2094505
display id: range2094505
version: 1
direction: direction/forward
top level: BBa_K404250
start: 1274
end: 3664
orientation: inline

BBa_K404231
type: ComponentDefinition
persistent identity: BBa K404231
display id: BBa_K404231
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-587KO-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404107
type: ComponentDefinition
persistent identity: BBa K404107
display id: BBa_K404107
version: 1
was generated by: iGEM to SBOL conversion
description: Beta-Globin-Intron
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Beta-Globin-intron
sequence: https://synbiohub.org/public/igem/BBa_K404107_sequence/1

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093172
display id: annotation2093172
version: 1
top level: BBa_K404107
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404107/annotation2093172/range2093172/1

https://synbiohub.org/public/igem/BBa_K404107/annotation2093172/range2093172/1
type: Range
persistent identity: range2093172
display id: range2093172
version: 1
direction: direction/forward
top level: BBa_K404107
start: 1
end: 495
orientation: inline

BBa_K404205
type: ComponentDefinition
persistent identity: BBa K404205
display id: BBa_K404205
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-587-BAP
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: BAP-motif
sequence annotation: downstream 587
sequence annotation: upstream 587
sequence annotation: upstream 453
sequence annotation: TG Spacer
sequence annotation: GLS Spacer
sequence: https://synbiohub.org/public/igem/BBa_K404205_sequence/1

BAP-motif
type: SequenceAnnotation
persistent identity: annotation2078950
display id: annotation2078950
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078950/range2078950/1

https://synbiohub.org/public/igem/BBa_K404205/annotation2078950/range2078950/1
type: Range
persistent identity: range2078950
display id: range2078950
version: 1
direction: direction/forward
top level: BBa_K404205
start: 49
end: 93
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2078952
display id: annotation2078952
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078952/range2078952/1

https://synbiohub.org/public/igem/BBa_K404205/annotation2078952/range2078952/1
type: Range
persistent identity: range2078952
display id: range2078952
version: 1
direction: direction/forward
top level: BBa_K404205
start: 103
end: 123
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2078948
display id: annotation2078948
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078948/range2078948/1

https://synbiohub.org/public/igem/BBa_K404205/annotation2078948/range2078948/1
type: Range
persistent identity: range2078948
display id: range2078948
version: 1
direction: direction/forward
top level: BBa_K404205
start: 10
end: 42
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2078953
display id: annotation2078953
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078953/range2078953/1

https://synbiohub.org/public/igem/BBa_K404205/annotation2078953/range2078953/1
type: Range
persistent identity: range2078953
display id: range2078953
version: 1
direction: direction/forward
top level: BBa_K404205
start: 124
end: 132
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2078947
display id: annotation2078947
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078947/range2078947/1

https://synbiohub.org/public/igem/BBa_K404205/annotation2078947/range2078947/1
type: Range
persistent identity: range2078947
display id: range2078947
version: 1
direction: direction/forward
top level: BBa_K404205
start: 1
end: 9
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2078949
display id: annotation2078949
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078949/range2078949/1

https://synbiohub.org/public/igem/BBa_K404205/annotation2078949/range2078949/1
type: Range
persistent identity: range2078949
display id: range2078949
version: 1
direction: direction/forward
top level: BBa_K404205
start: 43
end: 48
orientation: inline

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2078951
display id: annotation2078951
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078951/range2078951/1

https://synbiohub.org/public/igem/BBa_K404205/annotation2078951/range2078951/1
type: Range
persistent identity: range2078951
display id: range2078951
version: 1
direction: direction/forward
top level: BBa_K404205
start: 94
end: 102
orientation: inline

BBa_K404252
type: ComponentDefinition
persistent identity: BBa K404252
display id: BBa_K404252
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence annotation: HSPG-KO
sequence annotation: VP1
sequence annotation: downstream 598
sequence annotation: VP3
sequence annotation: CMV promoter
sequence annotation: upstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404252_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094564
display id: annotation2094564
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094564/range2094564/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094564/range2094564/1
type: Range
persistent identity: range2094564
display id: range2094564
version: 1
direction: direction/forward
top level: BBa_K404252
start: 671
end: 2998
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094565
display id: annotation2094565
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094565/range2094565/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094565/range2094565/1
type: Range
persistent identity: range2094565
display id: range2094565
version: 1
direction: direction/forward
top level: BBa_K404252
start: 1079
end: 2875
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101878
display id: annotation2101878
version: 1
top level: BBa_K404252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2101878/range2101878/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2101878/range2101878/1
type: Range
persistent identity: range2101878
display id: range2101878
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2419
end: 2421
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094563
display id: annotation2094563
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094563/range2094563/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094563/range2094563/1
type: Range
persistent identity: range2094563
display id: range2094563
version: 1
direction: direction/forward
top level: BBa_K404252
start: 671
end: 2875
orientation: inline

downstream 598
type: SequenceAnnotation
persistent identity: annotation2094568
display id: annotation2094568
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094568/range2094568/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094568/range2094568/1
type: Range
persistent identity: range2094568
display id: range2094568
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2429
end: 2444
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094566
display id: annotation2094566
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094566/range2094566/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094566/range2094566/1
type: Range
persistent identity: range2094566
display id: range2094566
version: 1
direction: direction/forward
top level: BBa_K404252
start: 1274
end: 2875
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101877
display id: annotation2101877
version: 1
top level: BBa_K404252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2101877/range2101877/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2101877/range2101877/1
type: Range
persistent identity: range2101877
display id: range2101877
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2429
end: 2431
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094562
display id: annotation2094562
version: 1
top level: BBa_K404252
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094562/range2094562/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094562/range2094562/1
type: Range
persistent identity: range2094562
display id: range2094562
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2
end: 655
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094567
display id: annotation2094567
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094567/range2094567/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094567/range2094567/1
type: Range
persistent identity: range2094567
display id: range2094567
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2397
end: 2428
orientation: inline

BBa_K404216
type: ComponentDefinition
persistent identity: BBa K404216
display id: BBa_K404216
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-587-Empty
creator: Freiburg Bioware 2010
created: 2010-10-12T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404227
type: ComponentDefinition
persistent identity: BBa K404227
display id: BBa_K404227
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587-RGD)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep52
sequence annotation: VP2
sequence annotation: upstream 587
sequence annotation: RGD
sequence annotation: Rep40
sequence annotation: VP3
sequence annotation: Rep68
sequence annotation: VP1
sequence annotation: TG-spacer
sequence annotation: GLS-spacer
sequence annotation: p5TATAless
sequence annotation: Rep78
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404227_sequence/1

Rep52
type: SequenceAnnotation
persistent identity: annotation2099009
display id: annotation2099009
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099009/range2099009/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099009/range2099009/1
type: Range
persistent identity: range2099009
display id: range2099009
version: 1
direction: direction/forward
top level: BBa_K404227
start: 673
end: 1867
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2099018
display id: annotation2099018
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099018/range2099018/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099018/range2099018/1
type: Range
persistent identity: range2099018
display id: range2099018
version: 1
direction: direction/forward
top level: BBa_K404227
start: 2294
end: 4132
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2099029
display id: annotation2099029
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099029/range2099029/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099029/range2099029/1
type: Range
persistent identity: range2099029
display id: range2099029
version: 1
direction: direction/forward
top level: BBa_K404227
start: 3612
end: 3643
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2099023
display id: annotation2099023
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099023/range2099023/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099023/range2099023/1
type: Range
persistent identity: range2099023
display id: range2099023
version: 1
direction: direction/forward
top level: BBa_K404227
start: 3650
end: 3676
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2099010
display id: annotation2099010
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099010/range2099010/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099010/range2099010/1
type: Range
persistent identity: range2099010
display id: range2099010
version: 1
direction: direction/forward
top level: BBa_K404227
start: 673
end: 1933
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2099019
display id: annotation2099019
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099019/range2099019/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099019/range2099019/1
type: Range
persistent identity: range2099019
display id: range2099019
version: 1
direction: direction/forward
top level: BBa_K404227
start: 2489
end: 4132
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2099008
display id: annotation2099008
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099008/range2099008/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099008/range2099008/1
type: Range
persistent identity: range2099008
display id: range2099008
version: 1
direction: direction/forward
top level: BBa_K404227
start: 2
end: 1933
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2099012
display id: annotation2099012
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099012/range2099012/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099012/range2099012/1
type: Range
persistent identity: range2099012
display id: range2099012
version: 1
direction: direction/forward
top level: BBa_K404227
start: 1883
end: 4132
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2099024
display id: annotation2099024
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099024/range2099024/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099024/range2099024/1
type: Range
persistent identity: range2099024
display id: range2099024
version: 1
direction: direction/forward
top level: BBa_K404227
start: 3644
end: 3649
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2099028
display id: annotation2099028
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099028/range2099028/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099028/range2099028/1
type: Range
persistent identity: range2099028
display id: range2099028
version: 1
direction: direction/forward
top level: BBa_K404227
start: 3677
end: 3685
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2099021
display id: annotation2099021
version: 1
top level: BBa_K404227
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099021/range2099021/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099021/range2099021/1
type: Range
persistent identity: range2099021
display id: range2099021
version: 1
direction: direction/forward
top level: BBa_K404227
start: 4264
end: 4427
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2099007
display id: annotation2099007
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099007/range2099007/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099007/range2099007/1
type: Range
persistent identity: range2099007
display id: range2099007
version: 1
direction: direction/forward
top level: BBa_K404227
start: 2
end: 1867
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2099030
display id: annotation2099030
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099030/range2099030/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099030/range2099030/1
type: Range
persistent identity: range2099030
display id: range2099030
version: 1
direction: direction/forward
top level: BBa_K404227
start: 3686
end: 3701
orientation: inline

BBa_K404011
type: ComponentDefinition
persistent identity: BBa K404011
display id: BBa_K404011
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]-VP2
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404011_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2097904
display id: annotation2097904
version: 1
top level: BBa_K404011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404011/annotation2097904/range2097904/1

https://synbiohub.org/public/igem/BBa_K404011/annotation2097904/range2097904/1
type: Range
persistent identity: range2097904
display id: range2097904
version: 1
direction: direction/forward
top level: BBa_K404011
start: 670
end: 2500
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097903
display id: annotation2097903
version: 1
top level: BBa_K404011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404011/annotation2097903/range2097903/1

https://synbiohub.org/public/igem/BBa_K404011/annotation2097903/range2097903/1
type: Range
persistent identity: range2097903
display id: range2097903
version: 1
direction: direction/forward
top level: BBa_K404011
start: 2
end: 655
orientation: inline

BBa_K404160
type: ComponentDefinition
persistent identity: BBa K404160
display id: BBa_K404160
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_His-Tag_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: HSPG-KO
sequence annotation: His-tag
sequence annotation: VP3
sequence annotation: middle.linker
sequence annotation: VP2
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404160_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2097920
display id: annotation2097920
version: 1
top level: BBa_K404160
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097920/range2097920/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097920/range2097920/1
type: Range
persistent identity: range2097920
display id: range2097920
version: 1
direction: direction/forward
top level: BBa_K404160
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101800
display id: annotation2101800
version: 1
top level: BBa_K404160
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2101800/range2101800/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2101800/range2101800/1
type: Range
persistent identity: range2101800
display id: range2101800
version: 1
direction: direction/forward
top level: BBa_K404160
start: 2063
end: 2065
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2097921
display id: annotation2097921
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097921/range2097921/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097921/range2097921/1
type: Range
persistent identity: range2097921
display id: range2097921
version: 1
direction: direction/forward
top level: BBa_K404160
start: 671
end: 688
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097924
display id: annotation2097924
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097924/range2097924/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097924/range2097924/1
type: Range
persistent identity: range2097924
display id: range2097924
version: 1
direction: direction/forward
top level: BBa_K404160
start: 917
end: 2518
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101799
display id: annotation2101799
version: 1
top level: BBa_K404160
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2101799/range2101799/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2101799/range2101799/1
type: Range
persistent identity: range2101799
display id: range2101799
version: 1
direction: direction/forward
top level: BBa_K404160
start: 2072
end: 2074
orientation: inline

middle.linker
type: SequenceAnnotation
persistent identity: annotation2097922
display id: annotation2097922
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097922/range2097922/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097922/range2097922/1
type: Range
persistent identity: range2097922
display id: range2097922
version: 1
direction: direction/forward
top level: BBa_K404160
start: 695
end: 718
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097923
display id: annotation2097923
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097923/range2097923/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097923/range2097923/1
type: Range
persistent identity: range2097923
display id: range2097923
version: 1
direction: direction/forward
top level: BBa_K404160
start: 725
end: 2518
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097925
display id: annotation2097925
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097925/range2097925/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097925/range2097925/1
type: Range
persistent identity: range2097925
display id: range2097925
version: 1
direction: direction/forward
top level: BBa_K404160
start: 725
end: 2641
orientation: inline

BBa_K404230
type: ComponentDefinition
persistent identity: BBa K404230
display id: BBa_K404230
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404164
type: ComponentDefinition
persistent identity: BBa K404164
display id: BBa_K404164
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_VP1up_NLS_Z-EGFR-1907_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1up
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: CMV
sequence annotation: ZEGFR_1907
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: NLS
sequence: https://synbiohub.org/public/igem/BBa_K404164_sequence/1

VP1up
type: SequenceAnnotation
persistent identity: annotation2095192
display id: annotation2095192
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095192/range2095192/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095192/range2095192/1
type: Range
persistent identity: range2095192
display id: range2095192
version: 1
direction: direction/forward
top level: BBa_K404164
start: 673
end: 1081
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095195
display id: annotation2095195
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095195/range2095195/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095195/range2095195/1
type: Range
persistent identity: range2095195
display id: range2095195
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1487
end: 3088
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095199
display id: annotation2095199
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095199/range2095199/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095199/range2095199/1
type: Range
persistent identity: range2095199
display id: range2095199
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1295
end: 3211
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095191
display id: annotation2095191
version: 1
top level: BBa_K404164
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095191/range2095191/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095191/range2095191/1
type: Range
persistent identity: range2095191
display id: range2095191
version: 1
direction: direction/forward
top level: BBa_K404164
start: 2
end: 655
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2095193
display id: annotation2095193
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095193/range2095193/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095193/range2095193/1
type: Range
persistent identity: range2095193
display id: range2095193
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1115
end: 1228
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101815
display id: annotation2101815
version: 1
top level: BBa_K404164
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2101815/range2101815/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2101815/range2101815/1
type: Range
persistent identity: range2101815
display id: range2101815
version: 1
direction: direction/forward
top level: BBa_K404164
start: 2633
end: 2635
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095194
display id: annotation2095194
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095194/range2095194/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095194/range2095194/1
type: Range
persistent identity: range2095194
display id: range2095194
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1295
end: 3088
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2095197
display id: annotation2095197
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095197/range2095197/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095197/range2095197/1
type: Range
persistent identity: range2095197
display id: range2095197
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1088
end: 1108
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101814
display id: annotation2101814
version: 1
top level: BBa_K404164
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2101814/range2101814/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2101814/range2101814/1
type: Range
persistent identity: range2101814
display id: range2101814
version: 1
direction: direction/forward
top level: BBa_K404164
start: 2642
end: 2644
orientation: inline

BBa_K404004
type: ComponentDefinition
persistent identity: BBa K404004
display id: BBa_K404004
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP13(ViralBrick-587KO-empty)_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: basic region 3
sequence annotation: Rep40
sequence annotation: PLA2
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: G--> C VP2 KO
sequence annotation: HSPG-KO
sequence annotation: VP1
sequence annotation: basic region 4
sequence annotation: p19
sequence annotation: scar
sequence annotation: HSPK-KO
sequence annotation: Rep52
sequence annotation: Rep78
sequence annotation: Rep68
sequence annotation: VP3
sequence annotation: p5TATAless
sequence annotation: basic region 2
sequence annotation: basic region 1
sequence annotation: p40
sequence: https://synbiohub.org/public/igem/BBa_K404004_sequence/1

basic region 3
type: SequenceAnnotation
persistent identity: annotation2099620
display id: annotation2099620
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099620/range2099620/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099620/range2099620/1
type: Range
persistent identity: range2099620
display id: range2099620
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2378
end: 2398
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2099612
display id: annotation2099612
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099612/range2099612/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099612/range2099612/1
type: Range
persistent identity: range2099612
display id: range2099612
version: 1
direction: direction/forward
top level: BBa_K404004
start: 673
end: 1933
orientation: inline

PLA2
type: SequenceAnnotation
persistent identity: annotation2099617
display id: annotation2099617
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099617/range2099617/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099617/range2099617/1
type: Range
persistent identity: range2099617
display id: range2099617
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2015
end: 2191
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2099615
display id: annotation2099615
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099615/range2099615/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099615/range2099615/1
type: Range
persistent identity: range2099615
display id: range2099615
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1883
end: 4216
orientation: inline

G--> C VP2 KO
type: SequenceAnnotation
persistent identity: annotation2099624
display id: annotation2099624
version: 1
top level: BBa_K404004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099624/range2099624/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099624/range2099624/1
type: Range
persistent identity: range2099624
display id: range2099624
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2296
end: 2296
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2099626
display id: annotation2099626
version: 1
top level: BBa_K404004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099626/range2099626/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099626/range2099626/1
type: Range
persistent identity: range2099626
display id: range2099626
version: 1
direction: direction/forward
top level: BBa_K404004
start: 3635
end: 3637
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2099616
display id: annotation2099616
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099616/range2099616/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099616/range2099616/1
type: Range
persistent identity: range2099616
display id: range2099616
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1883
end: 4090
orientation: inline

basic region 4
type: SequenceAnnotation
persistent identity: annotation2099622
display id: annotation2099622
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099622/range2099622/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099622/range2099622/1
type: Range
persistent identity: range2099622
display id: range2099622
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2801
end: 2818
orientation: inline

p19
type: SequenceAnnotation
persistent identity: annotation2099611
display id: annotation2099611
version: 1
top level: BBa_K404004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099611/range2099611/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099611/range2099611/1
type: Range
persistent identity: range2099611
display id: range2099611
version: 1
direction: direction/forward
top level: BBa_K404004
start: 410
end: 529
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101675
display id: annotation2101675
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2101675/range2101675/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2101675/range2101675/1
type: Range
persistent identity: range2101675
display id: range2101675
version: 1
direction: direction/forward
top level: BBa_K404004
start: 4217
end: 4327
orientation: inline

HSPK-KO
type: SequenceAnnotation
persistent identity: annotation2099625
display id: annotation2099625
version: 1
top level: BBa_K404004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099625/range2099625/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099625/range2099625/1
type: Range
persistent identity: range2099625
display id: range2099625
version: 1
direction: direction/forward
top level: BBa_K404004
start: 3644
end: 3646
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2099613
display id: annotation2099613
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099613/range2099613/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099613/range2099613/1
type: Range
persistent identity: range2099613
display id: range2099613
version: 1
direction: direction/forward
top level: BBa_K404004
start: 673
end: 1867
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2099610
display id: annotation2099610
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099610/range2099610/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099610/range2099610/1
type: Range
persistent identity: range2099610
display id: range2099610
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1
end: 1867
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2099609
display id: annotation2099609
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099609/range2099609/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099609/range2099609/1
type: Range
persistent identity: range2099609
display id: range2099609
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1
end: 1933
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2099621
display id: annotation2099621
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099621/range2099621/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099621/range2099621/1
type: Range
persistent identity: range2099621
display id: range2099621
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2489
end: 4090
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2099623
display id: annotation2099623
version: 1
top level: BBa_K404004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099623/range2099623/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099623/range2099623/1
type: Range
persistent identity: range2099623
display id: range2099623
version: 1
direction: direction/forward
top level: BBa_K404004
start: 4328
end: 4401
orientation: inline

basic region 2
type: SequenceAnnotation
persistent identity: annotation2099619
display id: annotation2099619
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099619/range2099619/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099619/range2099619/1
type: Range
persistent identity: range2099619
display id: range2099619
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2300
end: 2320
orientation: inline

basic region 1
type: SequenceAnnotation
persistent identity: annotation2099618
display id: annotation2099618
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099618/range2099618/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099618/range2099618/1
type: Range
persistent identity: range2099618
display id: range2099618
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2240
end: 2260
orientation: inline

p40
type: SequenceAnnotation
persistent identity: annotation2099614
display id: annotation2099614
version: 1
top level: BBa_K404004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099614/range2099614/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099614/range2099614/1
type: Range
persistent identity: range2099614
display id: range2099614
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1390
end: 1507
orientation: inline

BBa_K404093
type: ComponentDefinition
persistent identity: BBa K404093
display id: BBa_K404093
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep78ex
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rep78
sequence: https://synbiohub.org/public/igem/BBa_K404093_sequence/1

Rep78
type: SequenceAnnotation
persistent identity: annotation2093163
display id: annotation2093163
version: 1
top level: BBa_K404093
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404093/annotation2093163/range2093163/1

https://synbiohub.org/public/igem/BBa_K404093/annotation2093163/range2093163/1
type: Range
persistent identity: range2093163
display id: range2093163
version: 1
direction: direction/forward
top level: BBa_K404093
start: 1
end: 1867
orientation: inline

BBa_K404092
type: ComponentDefinition
persistent identity: BBa K404092
display id: BBa_K404092
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep68ex
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep68
sequence: https://synbiohub.org/public/igem/BBa_K404092_sequence/1

Rep68
type: SequenceAnnotation
persistent identity: annotation2093162
display id: annotation2093162
version: 1
top level: BBa_K404092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404092/annotation2093162/range2093162/1

https://synbiohub.org/public/igem/BBa_K404092/annotation2093162/range2093162/1
type: Range
persistent identity: range2093162
display id: range2093162
version: 1
direction: direction/forward
top level: BBa_K404092
start: 1
end: 1612
orientation: inline

BBa_K404158
type: ComponentDefinition
persistent identity: BBa K404158
display id: BBa_K404158
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_Z-EGFR-1907_SEG-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: HSPG-KO
sequence annotation: SEG-linker
sequence annotation: ZEGFR_1907
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404158_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095005
display id: annotation2095005
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095005/range2095005/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095005/range2095005/1
type: Range
persistent identity: range2095005
display id: range2095005
version: 1
direction: direction/forward
top level: BBa_K404158
start: 965
end: 2881
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101795
display id: annotation2101795
version: 1
top level: BBa_K404158
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2101795/range2101795/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2101795/range2101795/1
type: Range
persistent identity: range2101795
display id: range2101795
version: 1
direction: direction/forward
top level: BBa_K404158
start: 2303
end: 2305
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101794
display id: annotation2101794
version: 1
top level: BBa_K404158
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2101794/range2101794/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2101794/range2101794/1
type: Range
persistent identity: range2101794
display id: range2101794
version: 1
direction: direction/forward
top level: BBa_K404158
start: 2312
end: 2314
orientation: inline

SEG-linker
type: SequenceAnnotation
persistent identity: annotation2095002
display id: annotation2095002
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095002/range2095002/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095002/range2095002/1
type: Range
persistent identity: range2095002
display id: range2095002
version: 1
direction: direction/forward
top level: BBa_K404158
start: 851
end: 858
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2095001
display id: annotation2095001
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095001/range2095001/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095001/range2095001/1
type: Range
persistent identity: range2095001
display id: range2095001
version: 1
direction: direction/forward
top level: BBa_K404158
start: 671
end: 844
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095004
display id: annotation2095004
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095004/range2095004/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095004/range2095004/1
type: Range
persistent identity: range2095004
display id: range2095004
version: 1
direction: direction/forward
top level: BBa_K404158
start: 1157
end: 2758
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095003
display id: annotation2095003
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095003/range2095003/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095003/range2095003/1
type: Range
persistent identity: range2095003
display id: range2095003
version: 1
direction: direction/forward
top level: BBa_K404158
start: 965
end: 2758
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095000
display id: annotation2095000
version: 1
top level: BBa_K404158
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095000/range2095000/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095000/range2095000/1
type: Range
persistent identity: range2095000
display id: range2095000
version: 1
direction: direction/forward
top level: BBa_K404158
start: 2
end: 655
orientation: inline

BBa_K404201
type: ComponentDefinition
persistent identity: BBa K404201
display id: BBa_K404201
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-453-BAP
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part
mutable notes: This part
mutable provenance: Synthetic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: A Spacer
sequence annotation: upstream 453
sequence annotation: BAP
sequence annotation: downstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404201_sequence/1

A Spacer
type: SequenceAnnotation
persistent identity: annotation2091219
display id: annotation2091219
version: 1
top level: BBa_K404201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404201/annotation2091219/range2091219/1

https://synbiohub.org/public/igem/BBa_K404201/annotation2091219/range2091219/1
type: Range
persistent identity: range2091219
display id: range2091219
version: 1
direction: direction/forward
top level: BBa_K404201
start: 43
end: 45
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2091218
display id: annotation2091218
version: 1
top level: BBa_K404201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404201/annotation2091218/range2091218/1

https://synbiohub.org/public/igem/BBa_K404201/annotation2091218/range2091218/1
type: Range
persistent identity: range2091218
display id: range2091218
version: 1
direction: direction/forward
top level: BBa_K404201
start: 1
end: 42
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2091220
display id: annotation2091220
version: 1
top level: BBa_K404201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404201/annotation2091220/range2091220/1

https://synbiohub.org/public/igem/BBa_K404201/annotation2091220/range2091220/1
type: Range
persistent identity: range2091220
display id: range2091220
version: 1
direction: direction/forward
top level: BBa_K404201
start: 46
end: 90
orientation: inline

A Spacer
type: SequenceAnnotation
persistent identity: annotation2091221
display id: annotation2091221
version: 1
top level: BBa_K404201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404201/annotation2091221/range2091221/1

https://synbiohub.org/public/igem/BBa_K404201/annotation2091221/range2091221/1
type: Range
persistent identity: range2091221
display id: range2091221
version: 1
direction: direction/forward
top level: BBa_K404201
start: 91
end: 93
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2091222
display id: annotation2091222
version: 1
top level: BBa_K404201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404201/annotation2091222/range2091222/1

https://synbiohub.org/public/igem/BBa_K404201/annotation2091222/range2091222/1
type: Range
persistent identity: range2091222
display id: range2091222
version: 1
direction: direction/forward
top level: BBa_K404201
start: 94
end: 114
orientation: inline

BBa_K404002
type: ComponentDefinition
persistent identity: BBa K404002
display id: BBa_K404002
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep68
sequence annotation: VP1
sequence annotation: Rep78
sequence annotation: p5TATAless
sequence annotation: Rep52
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: Rep40
sequence annotation: VP3
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404002_sequence/1

Rep68
type: SequenceAnnotation
persistent identity: annotation2097808
display id: annotation2097808
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097808/range2097808/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097808/range2097808/1
type: Range
persistent identity: range2097808
display id: range2097808
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1
end: 1931
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2097811
display id: annotation2097811
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097811/range2097811/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097811/range2097811/1
type: Range
persistent identity: range2097811
display id: range2097811
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1881
end: 4088
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2097807
display id: annotation2097807
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097807/range2097807/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097807/range2097807/1
type: Range
persistent identity: range2097807
display id: range2097807
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1
end: 1865
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2097816
display id: annotation2097816
version: 1
top level: BBa_K404002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097816/range2097816/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097816/range2097816/1
type: Range
persistent identity: range2097816
display id: range2097816
version: 1
direction: direction/forward
top level: BBa_K404002
start: 4220
end: 4383
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2097810
display id: annotation2097810
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097810/range2097810/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097810/range2097810/1
type: Range
persistent identity: range2097810
display id: range2097810
version: 1
direction: direction/forward
top level: BBa_K404002
start: 671
end: 1865
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097814
display id: annotation2097814
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097814/range2097814/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097814/range2097814/1
type: Range
persistent identity: range2097814
display id: range2097814
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1881
end: 4212
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2097809
display id: annotation2097809
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097809/range2097809/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097809/range2097809/1
type: Range
persistent identity: range2097809
display id: range2097809
version: 1
direction: direction/forward
top level: BBa_K404002
start: 671
end: 1931
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097813
display id: annotation2097813
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097813/range2097813/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097813/range2097813/1
type: Range
persistent identity: range2097813
display id: range2097813
version: 1
direction: direction/forward
top level: BBa_K404002
start: 2487
end: 4088
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097812
display id: annotation2097812
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097812/range2097812/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097812/range2097812/1
type: Range
persistent identity: range2097812
display id: range2097812
version: 1
direction: direction/forward
top level: BBa_K404002
start: 2292
end: 4088
orientation: inline

BBa_K404090
type: ComponentDefinition
persistent identity: BBa K404090
display id: BBa_K404090
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep40ex
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep40
sequence: https://synbiohub.org/public/igem/BBa_K404090_sequence/1

Rep40
type: SequenceAnnotation
persistent identity: annotation2093160
display id: annotation2093160
version: 1
top level: BBa_K404090
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404090/annotation2093160/range2093160/1

https://synbiohub.org/public/igem/BBa_K404090/annotation2093160/range2093160/1
type: Range
persistent identity: range2093160
display id: range2093160
version: 1
direction: direction/forward
top level: BBa_K404090
start: 1
end: 940
orientation: inline

BBa_K404150
type: ComponentDefinition
persistent identity: BBa K404150
display id: BBa_K404150
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-VP23
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404150_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2093338
display id: annotation2093338
version: 1
top level: BBa_K404150
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404150/annotation2093338/range2093338/1

https://synbiohub.org/public/igem/BBa_K404150/annotation2093338/range2093338/1
type: Range
persistent identity: range2093338
display id: range2093338
version: 1
direction: direction/forward
top level: BBa_K404150
start: 193
end: 1794
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2093337
display id: annotation2093337
version: 1
top level: BBa_K404150
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404150/annotation2093337/range2093337/1

https://synbiohub.org/public/igem/BBa_K404150/annotation2093337/range2093337/1
type: Range
persistent identity: range2093337
display id: range2093337
version: 1
direction: direction/forward
top level: BBa_K404150
start: 1
end: 1794
orientation: inline

BBa_K404236
type: ComponentDefinition
persistent identity: BBa K404236
display id: BBa_K404236
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]Rep-VP123_p5TATAless (ViralBrick 453 RGD-587 HSPG-KO)
creator: Freiburg Bioware 2010
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep78
sequence annotation: Rep68
sequence annotation: Rep40
sequence annotation: A-spacer
sequence annotation: RGD
sequence annotation: HSPG-KO
sequence annotation: Rep52
sequence annotation: VP3
sequence annotation: VP1
sequence annotation: VP2
sequence annotation: p5TATAless
sequence: https://synbiohub.org/public/igem/BBa_K404236_sequence/1

Rep78
type: SequenceAnnotation
persistent identity: annotation2098403
display id: annotation2098403
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098403/range2098403/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098403/range2098403/1
type: Range
persistent identity: range2098403
display id: range2098403
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2
end: 1867
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098405
display id: annotation2098405
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098405/range2098405/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098405/range2098405/1
type: Range
persistent identity: range2098405
display id: range2098405
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2
end: 1933
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098407
display id: annotation2098407
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098407/range2098407/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098407/range2098407/1
type: Range
persistent identity: range2098407
display id: range2098407
version: 1
direction: direction/forward
top level: BBa_K404236
start: 673
end: 1933
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098412
display id: annotation2098412
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098412/range2098412/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098412/range2098412/1
type: Range
persistent identity: range2098412
display id: range2098412
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3242
end: 3244
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2098411
display id: annotation2098411
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098411/range2098411/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098411/range2098411/1
type: Range
persistent identity: range2098411
display id: range2098411
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3245
end: 3271
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101874
display id: annotation2101874
version: 1
top level: BBa_K404236
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2101874/range2101874/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2101874/range2101874/1
type: Range
persistent identity: range2101874
display id: range2101874
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3155
end: 3157
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101873
display id: annotation2101873
version: 1
top level: BBa_K404236
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2101873/range2101873/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2101873/range2101873/1
type: Range
persistent identity: range2101873
display id: range2101873
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3308
end: 3310
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098413
display id: annotation2098413
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098413/range2098413/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098413/range2098413/1
type: Range
persistent identity: range2098413
display id: range2098413
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3272
end: 3274
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098406
display id: annotation2098406
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098406/range2098406/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098406/range2098406/1
type: Range
persistent identity: range2098406
display id: range2098406
version: 1
direction: direction/forward
top level: BBa_K404236
start: 673
end: 1867
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098410
display id: annotation2098410
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098410/range2098410/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098410/range2098410/1
type: Range
persistent identity: range2098410
display id: range2098410
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2489
end: 4123
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098408
display id: annotation2098408
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098408/range2098408/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098408/range2098408/1
type: Range
persistent identity: range2098408
display id: range2098408
version: 1
direction: direction/forward
top level: BBa_K404236
start: 1883
end: 4123
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098409
display id: annotation2098409
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098409/range2098409/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098409/range2098409/1
type: Range
persistent identity: range2098409
display id: range2098409
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2294
end: 4123
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098429
display id: annotation2098429
version: 1
top level: BBa_K404236
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098429/range2098429/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098429/range2098429/1
type: Range
persistent identity: range2098429
display id: range2098429
version: 1
direction: direction/forward
top level: BBa_K404236
start: 4255
end: 4418
orientation: inline

BBa_K404223
type: ComponentDefinition
persistent identity: BBa K404223
display id: BBa_K404223
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_p5-TATAless (ViralBrick-453-RGD)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: A-spacer
sequence annotation: VP2
sequence annotation: Rep68
sequence annotation: VP1
sequence annotation: RGD
sequence annotation: p5TATAless
sequence annotation: Rep40
sequence annotation: Rep52
sequence annotation: Rep78
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404223_sequence/1

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098399
display id: annotation2098399
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098399/range2098399/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098399/range2098399/1
type: Range
persistent identity: range2098399
display id: range2098399
version: 1
direction: direction/forward
top level: BBa_K404223
start: 3242
end: 3244
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098400
display id: annotation2098400
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098400/range2098400/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098400/range2098400/1
type: Range
persistent identity: range2098400
display id: range2098400
version: 1
direction: direction/forward
top level: BBa_K404223
start: 3272
end: 3274
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098370
display id: annotation2098370
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098370/range2098370/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098370/range2098370/1
type: Range
persistent identity: range2098370
display id: range2098370
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2294
end: 4123
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098340
display id: annotation2098340
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098340/range2098340/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098340/range2098340/1
type: Range
persistent identity: range2098340
display id: range2098340
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2
end: 1933
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098369
display id: annotation2098369
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098369/range2098369/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098369/range2098369/1
type: Range
persistent identity: range2098369
display id: range2098369
version: 1
direction: direction/forward
top level: BBa_K404223
start: 1883
end: 4123
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2098398
display id: annotation2098398
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098398/range2098398/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098398/range2098398/1
type: Range
persistent identity: range2098398
display id: range2098398
version: 1
direction: direction/forward
top level: BBa_K404223
start: 3245
end: 3271
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098397
display id: annotation2098397
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098397/range2098397/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098397/range2098397/1
type: Range
persistent identity: range2098397
display id: range2098397
version: 1
direction: direction/forward
top level: BBa_K404223
start: 4255
end: 4418
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098341
display id: annotation2098341
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098341/range2098341/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098341/range2098341/1
type: Range
persistent identity: range2098341
display id: range2098341
version: 1
direction: direction/forward
top level: BBa_K404223
start: 673
end: 1933
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098342
display id: annotation2098342
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098342/range2098342/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098342/range2098342/1
type: Range
persistent identity: range2098342
display id: range2098342
version: 1
direction: direction/forward
top level: BBa_K404223
start: 673
end: 1867
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098338
display id: annotation2098338
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098338/range2098338/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098338/range2098338/1
type: Range
persistent identity: range2098338
display id: range2098338
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2
end: 1867
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098396
display id: annotation2098396
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098396/range2098396/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098396/range2098396/1
type: Range
persistent identity: range2098396
display id: range2098396
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2489
end: 4123
orientation: inline

BBa_K404155
type: ComponentDefinition
persistent identity: BBa K404155
display id: BBa_K404155
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_Z-EGFR-1907_Short-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: Short-linker
sequence annotation: VP3
sequence annotation: CMV
sequence annotation: ZEGFR_1907
sequence: https://synbiohub.org/public/igem/BBa_K404155_sequence/1

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101721
display id: annotation2101721
version: 1
top level: BBa_K404155
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2101721/range2101721/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2101721/range2101721/1
type: Range
persistent identity: range2101721
display id: range2101721
version: 1
direction: direction/forward
top level: BBa_K404155
start: 2207
end: 2209
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094955
display id: annotation2094955
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094955/range2094955/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094955/range2094955/1
type: Range
persistent identity: range2094955
display id: range2094955
version: 1
direction: direction/forward
top level: BBa_K404155
start: 869
end: 2662
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094957
display id: annotation2094957
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094957/range2094957/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094957/range2094957/1
type: Range
persistent identity: range2094957
display id: range2094957
version: 1
direction: direction/forward
top level: BBa_K404155
start: 869
end: 2785
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101720
display id: annotation2101720
version: 1
top level: BBa_K404155
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2101720/range2101720/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2101720/range2101720/1
type: Range
persistent identity: range2101720
display id: range2101720
version: 1
direction: direction/forward
top level: BBa_K404155
start: 2216
end: 2218
orientation: inline

Short-linker
type: SequenceAnnotation
persistent identity: annotation2094954
display id: annotation2094954
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094954/range2094954/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094954/range2094954/1
type: Range
persistent identity: range2094954
display id: range2094954
version: 1
direction: direction/forward
top level: BBa_K404155
start: 851
end: 862
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094956
display id: annotation2094956
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094956/range2094956/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094956/range2094956/1
type: Range
persistent identity: range2094956
display id: range2094956
version: 1
direction: direction/forward
top level: BBa_K404155
start: 1061
end: 2662
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094952
display id: annotation2094952
version: 1
top level: BBa_K404155
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094952/range2094952/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094952/range2094952/1
type: Range
persistent identity: range2094952
display id: range2094952
version: 1
direction: direction/forward
top level: BBa_K404155
start: 2
end: 655
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2094953
display id: annotation2094953
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094953/range2094953/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094953/range2094953/1
type: Range
persistent identity: range2094953
display id: range2094953
version: 1
direction: direction/forward
top level: BBa_K404155
start: 641
end: 844
orientation: inline

BBa_K404105
type: ComponentDefinition
persistent identity: BBa K404105
display id: BBa_K404105
version: 1
was generated by: iGEM to SBOL conversion
description: p40 promoter
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: p40 promoter
sequence: https://synbiohub.org/public/igem/BBa_K404105_sequence/1

p40 promoter
type: SequenceAnnotation
persistent identity: annotation2093170
display id: annotation2093170
version: 1
top level: BBa_K404105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404105/annotation2093170/range2093170/1

https://synbiohub.org/public/igem/BBa_K404105/annotation2093170/range2093170/1
type: Range
persistent identity: range2093170
display id: range2093170
version: 1
direction: direction/forward
top level: BBa_K404105
start: 1
end: 182
orientation: inline

BBa_K404207
type: ComponentDefinition
persistent identity: BBa K404207
display id: BBa_K404207
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-587-RGD
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: GLS Spacer
sequence annotation: upstream 453
sequence annotation: downstream 587
sequence annotation: RGD-motif
sequence annotation: upstream 587
sequence annotation: TG Spacer
sequence: https://synbiohub.org/public/igem/BBa_K404207_sequence/1

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2079108
display id: annotation2079108
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079108/range2079108/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079108/range2079108/1
type: Range
persistent identity: range2079108
display id: range2079108
version: 1
direction: direction/forward
top level: BBa_K404207
start: 76
end: 84
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079104
display id: annotation2079104
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079104/range2079104/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079104/range2079104/1
type: Range
persistent identity: range2079104
display id: range2079104
version: 1
direction: direction/forward
top level: BBa_K404207
start: 1
end: 9
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079109
display id: annotation2079109
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079109/range2079109/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079109/range2079109/1
type: Range
persistent identity: range2079109
display id: range2079109
version: 1
direction: direction/forward
top level: BBa_K404207
start: 85
end: 105
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079110
display id: annotation2079110
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079110/range2079110/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079110/range2079110/1
type: Range
persistent identity: range2079110
display id: range2079110
version: 1
direction: direction/forward
top level: BBa_K404207
start: 106
end: 114
orientation: inline

RGD-motif
type: SequenceAnnotation
persistent identity: annotation2079107
display id: annotation2079107
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079107/range2079107/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079107/range2079107/1
type: Range
persistent identity: range2079107
display id: range2079107
version: 1
direction: direction/forward
top level: BBa_K404207
start: 49
end: 75
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079105
display id: annotation2079105
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079105/range2079105/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079105/range2079105/1
type: Range
persistent identity: range2079105
display id: range2079105
version: 1
direction: direction/forward
top level: BBa_K404207
start: 10
end: 42
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2079106
display id: annotation2079106
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079106/range2079106/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079106/range2079106/1
type: Range
persistent identity: range2079106
display id: range2079106
version: 1
direction: direction/forward
top level: BBa_K404207
start: 43
end: 48
orientation: inline

BBa_K404153
type: ComponentDefinition
persistent identity: BBa K404153
display id: BBa_K404153
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-NLS
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: NLS
sequence: https://synbiohub.org/public/igem/BBa_K404153_sequence/1

NLS
type: SequenceAnnotation
persistent identity: annotation2094093
display id: annotation2094093
version: 1
top level: BBa_K404153
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404153/annotation2094093/range2094093/1

https://synbiohub.org/public/igem/BBa_K404153/annotation2094093/range2094093/1
type: Range
persistent identity: range2094093
display id: range2094093
version: 1
direction: direction/forward
top level: BBa_K404153
start: 1
end: 21
orientation: inline

BBa_K404232
type: ComponentDefinition
persistent identity: BBa K404232
display id: BBa_K404232
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-RGD)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep78
sequence annotation: TG-spacer
sequence annotation: VP3
sequence annotation: p5TATAless
sequence annotation: downstream 587
sequence annotation: HSPG-KO
sequence annotation: upstream 587
sequence annotation: Rep40
sequence annotation: Rep52
sequence annotation: VP2
sequence annotation: RGD
sequence annotation: Rep68
sequence annotation: VP1
sequence annotation: GLS-spacer
sequence: https://synbiohub.org/public/igem/BBa_K404232_sequence/1

Rep78
type: SequenceAnnotation
persistent identity: annotation2098519
display id: annotation2098519
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098519/range2098519/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098519/range2098519/1
type: Range
persistent identity: range2098519
display id: range2098519
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2
end: 1867
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2098547
display id: annotation2098547
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098547/range2098547/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098547/range2098547/1
type: Range
persistent identity: range2098547
display id: range2098547
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3644
end: 3649
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098526
display id: annotation2098526
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098526/range2098526/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098526/range2098526/1
type: Range
persistent identity: range2098526
display id: range2098526
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2489
end: 4132
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098545
display id: annotation2098545
version: 1
top level: BBa_K404232
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098545/range2098545/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098545/range2098545/1
type: Range
persistent identity: range2098545
display id: range2098545
version: 1
direction: direction/forward
top level: BBa_K404232
start: 4264
end: 4427
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2098550
display id: annotation2098550
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098550/range2098550/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098550/range2098550/1
type: Range
persistent identity: range2098550
display id: range2098550
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3686
end: 3701
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101868
display id: annotation2101868
version: 1
top level: BBa_K404232
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2101868/range2101868/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2101868/range2101868/1
type: Range
persistent identity: range2101868
display id: range2101868
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3635
end: 3637
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2098549
display id: annotation2098549
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098549/range2098549/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098549/range2098549/1
type: Range
persistent identity: range2098549
display id: range2098549
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3612
end: 3643
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098522
display id: annotation2098522
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098522/range2098522/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098522/range2098522/1
type: Range
persistent identity: range2098522
display id: range2098522
version: 1
direction: direction/forward
top level: BBa_K404232
start: 673
end: 1933
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101867
display id: annotation2101867
version: 1
top level: BBa_K404232
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2101867/range2101867/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2101867/range2101867/1
type: Range
persistent identity: range2101867
display id: range2101867
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3678
end: 3680
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098521
display id: annotation2098521
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098521/range2098521/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098521/range2098521/1
type: Range
persistent identity: range2098521
display id: range2098521
version: 1
direction: direction/forward
top level: BBa_K404232
start: 673
end: 1867
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098525
display id: annotation2098525
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098525/range2098525/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098525/range2098525/1
type: Range
persistent identity: range2098525
display id: range2098525
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2294
end: 4132
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2098546
display id: annotation2098546
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098546/range2098546/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098546/range2098546/1
type: Range
persistent identity: range2098546
display id: range2098546
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3650
end: 3676
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098520
display id: annotation2098520
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098520/range2098520/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098520/range2098520/1
type: Range
persistent identity: range2098520
display id: range2098520
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2
end: 1933
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098523
display id: annotation2098523
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098523/range2098523/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098523/range2098523/1
type: Range
persistent identity: range2098523
display id: range2098523
version: 1
direction: direction/forward
top level: BBa_K404232
start: 1883
end: 4132
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2098548
display id: annotation2098548
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098548/range2098548/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098548/range2098548/1
type: Range
persistent identity: range2098548
display id: range2098548
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3677
end: 3685
orientation: inline

BBa_K404114
type: ComponentDefinition
persistent identity: BBa K404114
display id: BBa_K404114
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_pCMV
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: a
mutable provenance: s
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV promoter
sequence annotation: left ITR
sequence: https://synbiohub.org/public/igem/BBa_K404114_sequence/1

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093180
display id: annotation2093180
version: 1
top level: BBa_K404114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404114/annotation2093180/range2093180/1

https://synbiohub.org/public/igem/BBa_K404114/annotation2093180/range2093180/1
type: Range
persistent identity: range2093180
display id: range2093180
version: 1
direction: direction/forward
top level: BBa_K404114
start: 153
end: 809
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093179
display id: annotation2093179
version: 1
top level: BBa_K404114
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404114/annotation2093179/range2093179/1

https://synbiohub.org/public/igem/BBa_K404114/annotation2093179/range2093179/1
type: Range
persistent identity: range2093179
display id: range2093179
version: 1
direction: direction/forward
top level: BBa_K404114
start: 1
end: 147
orientation: inline

BBa_K404094
type: ComponentDefinition
persistent identity: BBa K404094
display id: BBa_K404094
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-AAPex
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K404094_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2093164
display id: annotation2093164
version: 1
top level: BBa_K404094
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404094/annotation2093164/range2093164/1

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type: Range
persistent identity: range2093164
display id: range2093164
version: 1
direction: direction/forward
top level: BBa_K404094
start: 1
end: 616
orientation: inline

BBa_K404001
type: ComponentDefinition
persistent identity: BBa K404001
display id: BBa_K404001
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123
creator: Team Freiburg 2010
created: 2010-08-22T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Test long
mutable notes: Test design considerations
mutable provenance: Test source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep40
sequence annotation: Rep52
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: Rep78
sequence annotation: VP1
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: Rep68
sequence: https://synbiohub.org/public/igem/BBa_K404001_sequence/1

Rep40
type: SequenceAnnotation
persistent identity: annotation2097894
display id: annotation2097894
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097894/range2097894/1

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type: Range
persistent identity: range2097894
display id: range2097894
version: 1
direction: direction/forward
top level: BBa_K404001
start: 673
end: 1933
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2097893
display id: annotation2097893
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097893/range2097893/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097893/range2097893/1
type: Range
persistent identity: range2097893
display id: range2097893
version: 1
direction: direction/forward
top level: BBa_K404001
start: 673
end: 1867
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097900
display id: annotation2097900
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097900/range2097900/1

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type: Range
persistent identity: range2097900
display id: range2097900
version: 1
direction: direction/forward
top level: BBa_K404001
start: 1883
end: 4213
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2097891
display id: annotation2097891
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097891/range2097891/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097891/range2097891/1
type: Range
persistent identity: range2097891
display id: range2097891
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2
end: 1867
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2097895
display id: annotation2097895
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097895/range2097895/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097895/range2097895/1
type: Range
persistent identity: range2097895
display id: range2097895
version: 1
direction: direction/forward
top level: BBa_K404001
start: 1883
end: 4090
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097896
display id: annotation2097896
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097896/range2097896/1

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type: Range
persistent identity: range2097896
display id: range2097896
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2294
end: 4090
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097899
display id: annotation2097899
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097899/range2097899/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097899/range2097899/1
type: Range
persistent identity: range2097899
display id: range2097899
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2489
end: 4090
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2097892
display id: annotation2097892
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097892/range2097892/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097892/range2097892/1
type: Range
persistent identity: range2097892
display id: range2097892
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2
end: 1933
orientation: inline

BBa_K404115
type: ComponentDefinition
persistent identity: BBa K404115
display id: BBa_K404115
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_phTERT
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: left ITR
sequence annotation: phTERT
sequence: https://synbiohub.org/public/igem/BBa_K404115_sequence/1

left ITR
type: SequenceAnnotation
persistent identity: annotation2093185
display id: annotation2093185
version: 1
top level: BBa_K404115
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404115/annotation2093185/range2093185/1

https://synbiohub.org/public/igem/BBa_K404115/annotation2093185/range2093185/1
type: Range
persistent identity: range2093185
display id: range2093185
version: 1
direction: direction/forward
top level: BBa_K404115
start: 1
end: 147
orientation: inline

phTERT
type: SequenceAnnotation
persistent identity: annotation2093186
display id: annotation2093186
version: 1
top level: BBa_K404115
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404115/annotation2093186/range2093186/1

https://synbiohub.org/public/igem/BBa_K404115/annotation2093186/range2093186/1
type: Range
persistent identity: range2093186
display id: range2093186
version: 1
direction: direction/forward
top level: BBa_K404115
start: 151
end: 612
orientation: inline

BBa_K404241
type: ComponentDefinition
persistent identity: BBa K404241
display id: BBa_K404241
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV_[AAV2]VP123 (ViralBrick-453-BAP)
creator: Freiburg Bioware 2010
created: 2010-09-19T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: BAP
sequence annotation: BR4
sequence annotation: PLA2
sequence annotation: VP1
sequence annotation: A-spacer
sequence annotation: BR2
sequence annotation: BR3
sequence annotation: CMV
sequence annotation: BR1
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: A-Spacer
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404241_sequence/1

BAP
type: SequenceAnnotation
persistent identity: annotation2094204
display id: annotation2094204
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094204/range2094204/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094204/range2094204/1
type: Range
persistent identity: range2094204
display id: range2094204
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2030
end: 2074
orientation: inline

BR4
type: SequenceAnnotation
persistent identity: annotation2094202
display id: annotation2094202
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094202/range2094202/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094202/range2094202/1
type: Range
persistent identity: range2094202
display id: range2094202
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1586
end: 1603
orientation: inline

PLA2
type: SequenceAnnotation
persistent identity: annotation2094196
display id: annotation2094196
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094196/range2094196/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094196/range2094196/1
type: Range
persistent identity: range2094196
display id: range2094196
version: 1
direction: direction/forward
top level: BBa_K404241
start: 799
end: 976
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094195
display id: annotation2094195
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094195/range2094195/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094195/range2094195/1
type: Range
persistent identity: range2094195
display id: range2094195
version: 1
direction: direction/forward
top level: BBa_K404241
start: 671
end: 2926
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2094191
display id: annotation2094191
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094191/range2094191/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094191/range2094191/1
type: Range
persistent identity: range2094191
display id: range2094191
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2075
end: 2077
orientation: inline

BR2
type: SequenceAnnotation
persistent identity: annotation2094199
display id: annotation2094199
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094199/range2094199/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094199/range2094199/1
type: Range
persistent identity: range2094199
display id: range2094199
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1085
end: 1105
orientation: inline

BR3
type: SequenceAnnotation
persistent identity: annotation2094200
display id: annotation2094200
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094200/range2094200/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094200/range2094200/1
type: Range
persistent identity: range2094200
display id: range2094200
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1163
end: 1183
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094193
display id: annotation2094193
version: 1
top level: BBa_K404241
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094193/range2094193/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094193/range2094193/1
type: Range
persistent identity: range2094193
display id: range2094193
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2
end: 655
orientation: inline

BR1
type: SequenceAnnotation
persistent identity: annotation2094197
display id: annotation2094197
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094197/range2094197/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094197/range2094197/1
type: Range
persistent identity: range2094197
display id: range2094197
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1025
end: 1045
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094201
display id: annotation2094201
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094201/range2094201/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094201/range2094201/1
type: Range
persistent identity: range2094201
display id: range2094201
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1274
end: 2926
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094198
display id: annotation2094198
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094198/range2094198/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094198/range2094198/1
type: Range
persistent identity: range2094198
display id: range2094198
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1079
end: 2926
orientation: inline

A-Spacer
type: SequenceAnnotation
persistent identity: annotation2094203
display id: annotation2094203
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094203/range2094203/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094203/range2094203/1
type: Range
persistent identity: range2094203
display id: range2094203
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2027
end: 2029
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094194
display id: annotation2094194
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094194/range2094194/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094194/range2094194/1
type: Range
persistent identity: range2094194
display id: range2094194
version: 1
direction: direction/forward
top level: BBa_K404241
start: 671
end: 3049
orientation: inline

BBa_K404234
type: ComponentDefinition
persistent identity: BBa K404234
display id: BBa_K404234
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-Z34C-Spacer)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: upstream 587
sequence annotation: GLS-spacer
sequence annotation: TG-spacer
sequence annotation: Z34C
sequence annotation: downstream 587
sequence annotation: HSPG-KO
sequence annotation: Rep78
sequence annotation: Rep52
sequence annotation: VP1
sequence annotation: Rep68
sequence annotation: VP2
sequence annotation: p5TATAless
sequence annotation: Rep40
sequence: https://synbiohub.org/public/igem/BBa_K404234_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2098437
display id: annotation2098437
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098437/range2098437/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098437/range2098437/1
type: Range
persistent identity: range2098437
display id: range2098437
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2489
end: 4207
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2098435
display id: annotation2098435
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098435/range2098435/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098435/range2098435/1
type: Range
persistent identity: range2098435
display id: range2098435
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3612
end: 3643
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2098479
display id: annotation2098479
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098479/range2098479/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098479/range2098479/1
type: Range
persistent identity: range2098479
display id: range2098479
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3752
end: 3760
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2098460
display id: annotation2098460
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098460/range2098460/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098460/range2098460/1
type: Range
persistent identity: range2098460
display id: range2098460
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3644
end: 3649
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2098439
display id: annotation2098439
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098439/range2098439/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098439/range2098439/1
type: Range
persistent identity: range2098439
display id: range2098439
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3650
end: 3751
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2098500
display id: annotation2098500
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098500/range2098500/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098500/range2098500/1
type: Range
persistent identity: range2098500
display id: range2098500
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3761
end: 3776
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101870
display id: annotation2101870
version: 1
top level: BBa_K404234
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2101870/range2101870/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2101870/range2101870/1
type: Range
persistent identity: range2101870
display id: range2101870
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3648
end: 3650
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101869
display id: annotation2101869
version: 1
top level: BBa_K404234
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2101869/range2101869/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2101869/range2101869/1
type: Range
persistent identity: range2101869
display id: range2101869
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3753
end: 3755
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098430
display id: annotation2098430
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098430/range2098430/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098430/range2098430/1
type: Range
persistent identity: range2098430
display id: range2098430
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2
end: 1867
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098432
display id: annotation2098432
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098432/range2098432/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098432/range2098432/1
type: Range
persistent identity: range2098432
display id: range2098432
version: 1
direction: direction/forward
top level: BBa_K404234
start: 673
end: 1867
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098434
display id: annotation2098434
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098434/range2098434/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098434/range2098434/1
type: Range
persistent identity: range2098434
display id: range2098434
version: 1
direction: direction/forward
top level: BBa_K404234
start: 1883
end: 4207
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098431
display id: annotation2098431
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098431/range2098431/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098431/range2098431/1
type: Range
persistent identity: range2098431
display id: range2098431
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2
end: 1933
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098436
display id: annotation2098436
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098436/range2098436/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098436/range2098436/1
type: Range
persistent identity: range2098436
display id: range2098436
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2294
end: 4207
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098438
display id: annotation2098438
version: 1
top level: BBa_K404234
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098438/range2098438/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098438/range2098438/1
type: Range
persistent identity: range2098438
display id: range2098438
version: 1
direction: direction/forward
top level: BBa_K404234
start: 4339
end: 4502
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098433
display id: annotation2098433
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098433/range2098433/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098433/range2098433/1
type: Range
persistent identity: range2098433
display id: range2098433
version: 1
direction: direction/forward
top level: BBa_K404234
start: 673
end: 1933
orientation: inline

BBa_K404012
type: ComponentDefinition
persistent identity: BBa K404012
display id: BBa_K404012
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-VP3
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404012_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2109186
display id: annotation2109186
version: 1
top level: BBa_K404012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404012/annotation2109186/range2109186/1

https://synbiohub.org/public/igem/BBa_K404012/annotation2109186/range2109186/1
type: Range
persistent identity: range2109186
display id: range2109186
version: 1
direction: direction/forward
top level: BBa_K404012
start: 1
end: 1635
orientation: inline

BBa_K404120
type: ComponentDefinition
persistent identity: BBa K404120
display id: BBa_K404120
version: 1
was generated by: iGEM to SBOL conversion
description: [AAV2]-left-ITR_pCMV_betaglobin_CFP_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Beta-Globin-intron
sequence annotation: CFP
sequence annotation: left ITR
sequence annotation: hGH
sequence annotation: CMV promoter
sequence annotation: right ITR
sequence: https://synbiohub.org/public/igem/BBa_K404120_sequence/1

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093246
display id: annotation2093246
version: 1
top level: BBa_K404120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093246/range2093246/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093246/range2093246/1
type: Range
persistent identity: range2093246
display id: range2093246
version: 1
direction: direction/forward
top level: BBa_K404120
start: 816
end: 1310
orientation: inline

CFP
type: SequenceAnnotation
persistent identity: annotation2093247
display id: annotation2093247
version: 1
top level: BBa_K404120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093247/range2093247/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093247/range2093247/1
type: Range
persistent identity: range2093247
display id: range2093247
version: 1
direction: direction/forward
top level: BBa_K404120
start: 1325
end: 2038
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093244
display id: annotation2093244
version: 1
top level: BBa_K404120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093244/range2093244/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093244/range2093244/1
type: Range
persistent identity: range2093244
display id: range2093244
version: 1
direction: direction/forward
top level: BBa_K404120
start: 1
end: 147
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2093257
display id: annotation2093257
version: 1
top level: BBa_K404120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093257/range2093257/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093257/range2093257/1
type: Range
persistent identity: range2093257
display id: range2093257
version: 1
direction: direction/forward
top level: BBa_K404120
start: 2056
end: 2534
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093245
display id: annotation2093245
version: 1
top level: BBa_K404120
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093245/range2093245/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093245/range2093245/1
type: Range
persistent identity: range2093245
display id: range2093245
version: 1
direction: direction/forward
top level: BBa_K404120
start: 155
end: 809
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093258
display id: annotation2093258
version: 1
top level: BBa_K404120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093258/range2093258/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093258/range2093258/1
type: Range
persistent identity: range2093258
display id: range2093258
version: 1
direction: direction/forward
top level: BBa_K404120
start: 2549
end: 2686
orientation: inline

BBa_K404108
type: ComponentDefinition
persistent identity: BBa K404108
display id: BBa_K404108
version: 1
was generated by: iGEM to SBOL conversion
description: hGH terminator
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
mutable provenance: x
owned by: james
owned by: myers
mutable description: x
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: x
star: false
creator: Freiburg Bioware 2010
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: hGH terminator
sequence: https://synbiohub.org/public/igem/BBa_K404108_sequence/1

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2093173
display id: annotation2093173
version: 1
top level: BBa_K404108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404108/annotation2093173/range2093173/1

https://synbiohub.org/public/igem/BBa_K404108/annotation2093173/range2093173/1
type: Range
persistent identity: range2093173
display id: range2093173
version: 1
top level: BBa_K404108
direction: direction/forward
start: 1
end: 481
orientation: inline

BBa_K404214
type: ComponentDefinition
persistent identity: BBa K404214
display id: BBa_K404214
version: 1
was generated by: iGEM to SBOL conversion
description: ViralBrick-587KO-Z34C-Spacer
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: R=>A
sequence annotation: downstream 587
sequence annotation: upstream 453
sequence annotation: downstream 453
sequence annotation: TG Spacer
sequence annotation: Z34C-motif
sequence annotation: upstream 587
sequence annotation: GLS Spacer
sequence: https://synbiohub.org/public/igem/BBa_K404214_sequence/1

R=>A
type: SequenceAnnotation
persistent identity: annotation2079172
display id: annotation2079172
version: 1
top level: BBa_K404214
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079172/range2079172/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079172/range2079172/1
type: Range
persistent identity: range2079172
display id: range2079172
version: 1
direction: direction/forward
top level: BBa_K404214
start: 160
end: 162
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079170
display id: annotation2079170
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079170/range2079170/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079170/range2079170/1
type: Range
persistent identity: range2079170
display id: range2079170
version: 1
direction: direction/forward
top level: BBa_K404214
start: 160
end: 180
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079164
display id: annotation2079164
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079164/range2079164/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079164/range2079164/1
type: Range
persistent identity: range2079164
display id: range2079164
version: 1
direction: direction/forward
top level: BBa_K404214
start: 1
end: 9
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079171
display id: annotation2079171
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079171/range2079171/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079171/range2079171/1
type: Range
persistent identity: range2079171
display id: range2079171
version: 1
direction: direction/forward
top level: BBa_K404214
start: 181
end: 189
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2079167
display id: annotation2079167
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079167/range2079167/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079167/range2079167/1
type: Range
persistent identity: range2079167
display id: range2079167
version: 1
direction: direction/forward
top level: BBa_K404214
start: 43
end: 48
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079166
display id: annotation2079166
version: 1
top level: BBa_K404214
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079166/range2079166/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079166/range2079166/1
type: Range
persistent identity: range2079166
display id: range2079166
version: 1
direction: direction/forward
top level: BBa_K404214
start: 34
end: 36
orientation: inline

Z34C-motif
type: SequenceAnnotation
persistent identity: annotation2079168
display id: annotation2079168
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079168/range2079168/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079168/range2079168/1
type: Range
persistent identity: range2079168
display id: range2079168
version: 1
direction: direction/forward
top level: BBa_K404214
start: 49
end: 150
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079165
display id: annotation2079165
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079165/range2079165/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079165/range2079165/1
type: Range
persistent identity: range2079165
display id: range2079165
version: 1
direction: direction/forward
top level: BBa_K404214
start: 10
end: 42
orientation: inline

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2079169
display id: annotation2079169
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079169/range2079169/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079169/range2079169/1
type: Range
persistent identity: range2079169
display id: range2079169
version: 1
direction: direction/forward
top level: BBa_K404214
start: 151
end: 159
orientation: inline

https://synbiohub.org/public/igem/BBa_K404126_sequence/1
type: Sequence
persistent identity: BBa K404126 sequence
display id: BBa_K404126_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggcctactagagcgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattactagagcgaatcccggccgggaacggtgcattggaacgcggattccccgtgccaagagtgacgtaagtaccgcctatagagtctataggcccacaaaaaatgctttcttcttttaatatacttttttgtttatcttatttctaatactttccctaatctctttctttcagggcaataatgatacaatgtatcatgcctctttgcaccattctaaagaataacagtgataatttctgggttaaggcaatagcaatatttctgcatataaatatttctgcatataaattgtaactgatgtaagaggtttcatattgctaatagcagctacaatccagctaccattctgcttttattttatggttgggataaggctggattattctgagtccaagctaggcccttttgctaatcatgttcatacctcttatcttcctcccacagctcctgggcaacgtgctggtctgtgtgctggcccatcactttggcaaagaattgggattactagatggccggcgtgtcgaataacgctttacaaacaattattaacgcccggttaccaggcgaagaggggctgtggcagattcatcttcaggacggaaaaatcagcgccattgatgcgcaatccggcgtgatgcccataactgaaaacagcctggatgccgaacaaggtttagttataccgccgtttgtggagccacatattcacctggacaccacgcaaaccgccggacaaccgaactggaatcagtccggcacgctgtttgaaggcattgaacgctgggccgagcgcaaagcgttattaacccatgacgatgtgaaacaacgcgcatggcaaacgctgaaatggcagattgccaacggcattcagcatgtgcgtacccatgtcgatgtttcggatgcaacgctaactgcgctgaaagcaatgctggaagtgaagcaggaagtcgcgccgtggattgatctgcaaatcgtcgccttccctcaggaagggattttgtcgtatcccaacggtgaagcgttgctggaagaggcgttacgcttaggggcagatgtagtgggggcgattccgcattttgaatttacccgtgaatacggcgtggagtcgctgcataaaaccttcgccctggcgcaaaaatacgaccgtctcatcgacgttcactgtgatgagatcgatgacgagcagtcgcgct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K404119_sequence/1
type: Sequence
persistent identity: BBa K404119 sequence
display id: BBa_K404119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggcctactagagcgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattactagagcgaatcccggccgggaacggtgcattggaacgcggattccccgtgccaagagtgacgtaagtaccgcctatagagtctataggcccacaaaaaatgctttcttcttttaatatacttttttgtttatcttatttctaatactttccctaatctctttctttcagggcaataatgatacaatgtatcatgcctctttgcaccattctaaagaataacagtgataatttctgggttaaggcaatagcaatatttctgcatataaatatttctgcatataaattgtaactgatgtaagaggtttcatattgctaatagcagctacaatccagctaccattctgcttttattttatggttgggataaggctggattattctgagtccaagctaggcccttttgctaatcatgttcatacctcttatcttcctcccacagctcctgggcaacgtgctggtctgtgtgctggcccatcactttggcaaagaattgggattactagatggccggcatgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaactgatttgcaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgggctatggcctgcaatgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacaacagccataacgtgtatatcaccgcggataaacagaaaaacggcatcaaagcgaactttaaaatccgccacaacattgaagatggcggcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagctaccagagcaaactgagcaaagatccgaacgaaaaacgtgaccatatggtgctgctggaatttgtgaccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K404167_sequence/1
type: Sequence
persistent identity: BBa K404167 sequence
display id: BBa_K404167_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattactagatggccggcgctgccgatggttatcttccagattggctcgaggacactctctctgaaggaataagacagtggtggaagctcaaacctggcccaccaccaccaaagcccgcagagcggcataaggacgacagcaggggtcttgtgcttcctgggtacaagtacctcggacccttcaacggactcgacaagggagagccggtcaacgaggcagacgccgcggccctcgagcacgacaaagcctacgaccggcagctcgacagcggagacaacccgtacctcaagtacaaccacgccgacgcggagtttcaggagcgccttaaagaagatacgtcttttgggggcaacctcggacgagcagtcttccaggcgaaaaagagggttcttgaacctctgggcctggttgaggaacctgttaaggcgaccggccctgcaagaaaaagattgaataccggcatgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaactgatttgcaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgggctatggcctgcaatgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacaacagccataacgtgtatatcaccgcggataaacagaaaaacggcatcaaagcgaactttaaaatccgccacaacattgaagatggcggcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagctaccagagcaaactgagcaaagatccgaacgaaaaacgtgaccatatggtgctgctggaatttgtgaccgcggccggtattacccatggcatggatgaactgtataaaaccggcgctccgggaaaaaagaggccggtagagcactctcctgtggagccagactcctcctcgggaaccggaaaggcgggccagcagcctgcaagaaaaagattgaattttggtcagactggagacgcagactcagtacctgacccccagcctctcggacagccaccagcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K404108_sequence/1
type: Sequence
persistent identity: BBa K404108 sequence
display id: BBa_K404108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K404121_sequence/1
type: Sequence
persistent identity: BBa K404121 sequence
display id: BBa_K404121_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggcctactagagcgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattactagagcgaatcccggccgggaacggtgcattggaacgcggattccccgtgccaagagtgacgtaagtaccgcctatagagtctataggcccacaaaaaatgctttcttcttttaatatacttttttgtttatcttatttctaatactttccctaatctctttctttcagggcaataatgatacaatgtatcatgcctctttgcaccattctaaagaataacagtgataatttctgggttaaggcaatagcaatatttctgcatataaatatttctgcatataaattgtaactgatgtaagaggtttcatattgctaatagcagctacaatccagctaccattctgcttttattttatggttgggataaggctggattattctgagtccaagctaggcccttttgctaatcatgttcatacctcttatcttcctcccacagctcctgggcaacgtgctggtctgtgtgctggcccatcactttggcaaagaattgggattactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K404125_sequence/1
type: Sequence
persistent identity: BBa K404125 sequence
display id: BBa_K404125_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggcctactagagtggcccctccctcgggttaccccacagcctaggccgattcgacctctctccgctggggccctcgctggcgtccctgcaccctgggagcgcgagcggcgcgcgggcggggaagcgcggcccagacccccgggtccgcccggagcagctgcgctgtcggggccaggccgggctcccagtggattcgcgggcacagacgcccaggaccgcgctccccacgtggcggagggactggggacccgggcacccgtcctgccccttcaccttccagctccgcctcctccgcgcggaccccgccccgtcccgacccctcccgggtccccggcccagccccctccgggccctcccagcccctccccttcctttccgcggccccgccctctcctcgcggcgcgagtttcaggcagcgctgcgtcctgctgcgcacgtgggaagccctggccccggccacccccgcgattactagagcgaatcccggccgggaacggtgcattggaacgcggattccccgtgccaagagtgacgtaagtaccgcctatagagtctataggcccacaaaaaatgctttcttcttttaatatacttttttgtttatcttatttctaatactttccctaatctctttctttcagggcaataatgatacaatgtatcatgcctctttgcaccattctaaagaataacagtgataatttctgggttaaggcaatagcaatatttctgcatataaatatttctgcatataaattgtaactgatgtaagaggtttcatattgctaatagcagctacaatccagctaccattctgcttttattttatggttgggataaggctggattattctgagtccaagctaggcccttttgctaatcatgttcatacctcttatcttcctcccacagctcctgggcaacgtgctggtctgtgtgctggcccatcactttggcaaagaattgggattactagatggccggcgtgtcgaataacgctttacaaacaattattaacgcccggttaccaggcgaagaggggctgtggcagattcatcttcaggacggaaaaatcagcgccattgatgcgcaatccggcgtgatgcccataactgaaaacagcctggatgccgaacaaggtttagttataccgccgtttgtggagccacatattcacctggacaccacgcaaaccgccggacaaccgaactggaatcagtccggcacgctgtttgaaggcattgaacgctgggccgagcgcaaagcgttattaacccatgacgatgtgaaacaacgcgcatggcaaacgctgaaatggcagattgccaacggcattcagcatgtgcgtacccatgtcgatgtttcggatgcaacgctaactgcgctgaaagcaatgctggaagtgaagcaggaagtcgcgccgtggattgatctgcaaatcgtcgccttccctcaggaagggattttgtcgtatcccaacggtgaagcgttgctggaagaggcgttacgcttaggggcagatgtagtgggggcgattccgcattttgaatttacccgtgaatacggcgtggagtcgctgcataaaaccttcgccctggcgcaaaaatacgaccgtctcatcgacgttcactgtgatgagatcgatgacgagcagtcgcgctttgtcgaaaccgttgctgccctggcgcaccatgaaggcatgggcgcgcgagtcaccgccagccacaccacggcaatgcactcctataacggggcgtatacctcacgcctgttccgcttgctgaaaatgtccggtattaactttgtcgccaacccgctggtcaatattcatctgcaaggacgtttcgatacgtatcca…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z