//viral_vectors/aav/capsid_coding
type: Collection
persistent identity: viral vectors aav capsid coding
display id: viral_vectors_aav_capsid_coding
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K404250
member: BBa_K404255
member: BBa_K404006
member: BBa_K404164
member: BBa_K404204
member: BBa_K404001
member: BBa_K404246
member: BBa_K404241
member: BBa_K404155
member: BBa_K404248
member: BBa_K404013
member: BBa_K404232
member: BBa_K404004
member: BBa_K404253
member: BBa_K404227
member: BBa_K404008
member: BBa_K404150
member: BBa_K404206
member: BBa_K404167
member: BBa_K404223
member: BBa_K404158
member: BBa_K404162
member: BBa_K404228
member: BBa_K404215
member: BBa_K404011
member: BBa_K404235
member: BBa_K404252
member: BBa_K404222
member: BBa_K404229
member: BBa_K404157
member: BBa_K404005
member: BBa_K404160
member: BBa_K404216
member: BBa_K404221
member: BBa_K404010
member: BBa_K404161
member: BBa_K404234
member: BBa_K404251
member: BBa_K404151
member: BBa_K404156
member: BBa_K404242
member: BBa_K404247
member: BBa_K404226
member: BBa_K404233
member: BBa_K404254
member: BBa_K404168
member: BBa_K404224
member: BBa_K404007
member: BBa_K404163
member: BBa_K404154
member: BBa_K404012
member: BBa_K404210
member: BBa_K404231
member: BBa_K404236
member: BBa_K404003
member: BBa_K404244
member: BBa_K404314
member: BBa_K404009
member: BBa_K404159
member: BBa_K404166
member: BBa_K404153
member: BBa_K404243
member: BBa_K404256
member: BBa_K404002
member: BBa_K404230
member: BBa_K404165
member: BBa_K404225
member: BBa_K404152

BBa_K404006
type: ComponentDefinition
persistent identity: BBa K404006
display id: BBa_K404006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP123
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence annotation: VP2
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404006_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2092160
display id: annotation2092160
version: 1
top level: BBa_K404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404006/annotation2092160/range2092160/1

https://synbiohub.org/public/igem/BBa_K404006/annotation2092160/range2092160/1
type: Range
persistent identity: range2092160
display id: range2092160
version: 1
direction: direction/forward
top level: BBa_K404006
start: 1
end: 2208
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2092161
display id: annotation2092161
version: 1
top level: BBa_K404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404006/annotation2092161/range2092161/1

https://synbiohub.org/public/igem/BBa_K404006/annotation2092161/range2092161/1
type: Range
persistent identity: range2092161
display id: range2092161
version: 1
direction: direction/forward
top level: BBa_K404006
start: 412
end: 2208
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2092162
display id: annotation2092162
version: 1
top level: BBa_K404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404006/annotation2092162/range2092162/1

https://synbiohub.org/public/igem/BBa_K404006/annotation2092162/range2092162/1
type: Range
persistent identity: range2092162
display id: range2092162
version: 1
direction: direction/forward
top level: BBa_K404006
start: 631
end: 2208
orientation: inline

BBa_K404255
type: ComponentDefinition
persistent identity: BBa K404255
display id: BBa_K404255
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Z34C)
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: upstream 587
sequence annotation: VP1
sequence annotation: VP2
sequence annotation: downstream 587
sequence annotation: HSPG-KO
sequence annotation: Z34C
sequence annotation: CMV
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404255_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094722
display id: annotation2094722
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094722/range2094722/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094722/range2094722/1
type: Range
persistent identity: range2094722
display id: range2094722
version: 1
direction: direction/forward
top level: BBa_K404255
start: 671
end: 3100
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094724
display id: annotation2094724
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094724/range2094724/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094724/range2094724/1
type: Range
persistent identity: range2094724
display id: range2094724
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2397
end: 2428
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094719
display id: annotation2094719
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094719/range2094719/1

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type: Range
persistent identity: range2094719
display id: range2094719
version: 1
direction: direction/forward
top level: BBa_K404255
start: 671
end: 2977
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094720
display id: annotation2094720
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094720/range2094720/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094720/range2094720/1
type: Range
persistent identity: range2094720
display id: range2094720
version: 1
direction: direction/forward
top level: BBa_K404255
start: 1079
end: 2977
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094725
display id: annotation2094725
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094725/range2094725/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094725/range2094725/1
type: Range
persistent identity: range2094725
display id: range2094725
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2531
end: 2546
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101893
display id: annotation2101893
version: 1
top level: BBa_K404255
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2101893/range2101893/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2101893/range2101893/1
type: Range
persistent identity: range2101893
display id: range2101893
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2420
end: 2422
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2094723
display id: annotation2094723
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094723/range2094723/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094723/range2094723/1
type: Range
persistent identity: range2094723
display id: range2094723
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2429
end: 2530
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094718
display id: annotation2094718
version: 1
top level: BBa_K404255
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094718/range2094718/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094718/range2094718/1
type: Range
persistent identity: range2094718
display id: range2094718
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094721
display id: annotation2094721
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094721/range2094721/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094721/range2094721/1
type: Range
persistent identity: range2094721
display id: range2094721
version: 1
direction: direction/forward
top level: BBa_K404255
start: 1274
end: 2977
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101892
display id: annotation2101892
version: 1
top level: BBa_K404255
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2101892/range2101892/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2101892/range2101892/1
type: Range
persistent identity: range2101892
display id: range2101892
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2531
end: 2533
orientation: inline

BBa_K404155
type: ComponentDefinition
persistent identity: BBa K404155
display id: BBa_K404155
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_Short-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: ZEGFR_1907
sequence annotation: Short-linker
sequence annotation: VP2
sequence annotation: HSPG-KO
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404155_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2094952
display id: annotation2094952
version: 1
top level: BBa_K404155
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094952/range2094952/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094952/range2094952/1
type: Range
persistent identity: range2094952
display id: range2094952
version: 1
direction: direction/forward
top level: BBa_K404155
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094956
display id: annotation2094956
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094956/range2094956/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094956/range2094956/1
type: Range
persistent identity: range2094956
display id: range2094956
version: 1
direction: direction/forward
top level: BBa_K404155
start: 1061
end: 2662
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2094953
display id: annotation2094953
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094953/range2094953/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094953/range2094953/1
type: Range
persistent identity: range2094953
display id: range2094953
version: 1
direction: direction/forward
top level: BBa_K404155
start: 641
end: 844
orientation: inline

Short-linker
type: SequenceAnnotation
persistent identity: annotation2094954
display id: annotation2094954
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094954/range2094954/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094954/range2094954/1
type: Range
persistent identity: range2094954
display id: range2094954
version: 1
direction: direction/forward
top level: BBa_K404155
start: 851
end: 862
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094955
display id: annotation2094955
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094955/range2094955/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094955/range2094955/1
type: Range
persistent identity: range2094955
display id: range2094955
version: 1
direction: direction/forward
top level: BBa_K404155
start: 869
end: 2662
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101720
display id: annotation2101720
version: 1
top level: BBa_K404155
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2101720/range2101720/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2101720/range2101720/1
type: Range
persistent identity: range2101720
display id: range2101720
version: 1
direction: direction/forward
top level: BBa_K404155
start: 2216
end: 2218
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094957
display id: annotation2094957
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094957/range2094957/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094957/range2094957/1
type: Range
persistent identity: range2094957
display id: range2094957
version: 1
direction: direction/forward
top level: BBa_K404155
start: 869
end: 2785
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101721
display id: annotation2101721
version: 1
top level: BBa_K404155
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2101721/range2101721/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2101721/range2101721/1
type: Range
persistent identity: range2101721
display id: range2101721
version: 1
direction: direction/forward
top level: BBa_K404155
start: 2207
end: 2209
orientation: inline

BBa_K404254
type: ComponentDefinition
persistent identity: BBa K404254
display id: BBa_K404254
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-RGD)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: TG-spacer
sequence annotation: RGD
sequence annotation: VP1
sequence annotation: CMV
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: GLS-spacer
sequence annotation: upstream 587
sequence annotation: VP3
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404254_sequence/1

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094685
display id: annotation2094685
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094685/range2094685/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094685/range2094685/1
type: Range
persistent identity: range2094685
display id: range2094685
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2429
end: 2434
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2094684
display id: annotation2094684
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094684/range2094684/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094684/range2094684/1
type: Range
persistent identity: range2094684
display id: range2094684
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2435
end: 2461
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094680
display id: annotation2094680
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094680/range2094680/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094680/range2094680/1
type: Range
persistent identity: range2094680
display id: range2094680
version: 1
direction: direction/forward
top level: BBa_K404254
start: 671
end: 2917
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094679
display id: annotation2094679
version: 1
top level: BBa_K404254
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094679/range2094679/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094679/range2094679/1
type: Range
persistent identity: range2094679
display id: range2094679
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101886
display id: annotation2101886
version: 1
top level: BBa_K404254
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2101886/range2101886/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2101886/range2101886/1
type: Range
persistent identity: range2101886
display id: range2101886
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2471
end: 2473
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094681
display id: annotation2094681
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094681/range2094681/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094681/range2094681/1
type: Range
persistent identity: range2094681
display id: range2094681
version: 1
direction: direction/forward
top level: BBa_K404254
start: 1079
end: 2917
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094683
display id: annotation2094683
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094683/range2094683/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094683/range2094683/1
type: Range
persistent identity: range2094683
display id: range2094683
version: 1
direction: direction/forward
top level: BBa_K404254
start: 671
end: 3040
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094686
display id: annotation2094686
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094686/range2094686/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094686/range2094686/1
type: Range
persistent identity: range2094686
display id: range2094686
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2462
end: 2470
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094688
display id: annotation2094688
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094688/range2094688/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094688/range2094688/1
type: Range
persistent identity: range2094688
display id: range2094688
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2397
end: 2428
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094682
display id: annotation2094682
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094682/range2094682/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094682/range2094682/1
type: Range
persistent identity: range2094682
display id: range2094682
version: 1
direction: direction/forward
top level: BBa_K404254
start: 1274
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persistent identity: range2094687
display id: range2094687
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top level: BBa_K404254
start: 2471
end: 2486
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HSPG-KO
type: SequenceAnnotation
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display id: annotation2101887
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top level: BBa_K404254
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2101887/range2101887/1

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type: Range
persistent identity: range2101887
display id: range2101887
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2433
end: 2435
orientation: inline

BBa_K404206
type: ComponentDefinition
persistent identity: BBa K404206
display id: BBa_K404206
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587-His-Tag
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstream 453
sequence annotation: downstream 453
sequence annotation: His-Tag
sequence annotation: TG Spacer
sequence annotation: upstream 587
sequence annotation: GLS Spacer
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404206_sequence/1

upstream 453
type: SequenceAnnotation
persistent identity: annotation2078954
display id: annotation2078954
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078954/range2078954/1

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persistent identity: range2078954
display id: range2078954
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start: 1
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downstream 453
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persistent identity: range2078960
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His-Tag
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top level: BBa_K404206
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role: feature/misc
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type: Range
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direction: direction/forward
top level: BBa_K404206
start: 49
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orientation: inline

TG Spacer
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version: 1
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role: SO:0000110
role: feature/misc
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persistent identity: range2078956
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start: 43
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upstream 587
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persistent identity: range2078955
display id: range2078955
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GLS Spacer
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persistent identity: annotation2078958
display id: annotation2078958
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078958/range2078958/1

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type: Range
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display id: range2078958
version: 1
direction: direction/forward
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start: 67
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downstream 587
type: SequenceAnnotation
persistent identity: annotation2078959
display id: annotation2078959
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2078959
display id: range2078959
version: 1
direction: direction/forward
top level: BBa_K404206
start: 75
end: 96
orientation: inline

BBa_K404153
type: ComponentDefinition
persistent identity: BBa K404153
display id: BBa_K404153
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-NLS
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: NLS
sequence: https://synbiohub.org/public/igem/BBa_K404153_sequence/1

NLS
type: SequenceAnnotation
persistent identity: annotation2094093
display id: annotation2094093
version: 1
top level: BBa_K404153
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404153/annotation2094093/range2094093/1

https://synbiohub.org/public/igem/BBa_K404153/annotation2094093/range2094093/1
type: Range
persistent identity: range2094093
display id: range2094093
version: 1
direction: direction/forward
top level: BBa_K404153
start: 1
end: 21
orientation: inline

BBa_K404001
type: ComponentDefinition
persistent identity: BBa K404001
display id: BBa_K404001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123
creator: Team Freiburg 2010
created: 2010-08-22T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Test long
mutable notes: Test design considerations
mutable provenance: Test source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep68
sequence annotation: VP1
sequence annotation: VP2
sequence annotation: Rep52
sequence annotation: Rep78
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: Rep40
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404001_sequence/1

Rep68
type: SequenceAnnotation
persistent identity: annotation2097892
display id: annotation2097892
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097892/range2097892/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097892/range2097892/1
type: Range
persistent identity: range2097892
display id: range2097892
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2
end: 1933
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2097895
display id: annotation2097895
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097895/range2097895/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097895/range2097895/1
type: Range
persistent identity: range2097895
display id: range2097895
version: 1
direction: direction/forward
top level: BBa_K404001
start: 1883
end: 4090
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097896
display id: annotation2097896
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097896/range2097896/1

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type: Range
persistent identity: range2097896
display id: range2097896
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2294
end: 4090
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2097893
display id: annotation2097893
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097893/range2097893/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097893/range2097893/1
type: Range
persistent identity: range2097893
display id: range2097893
version: 1
direction: direction/forward
top level: BBa_K404001
start: 673
end: 1867
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2097891
display id: annotation2097891
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097891/range2097891/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097891/range2097891/1
type: Range
persistent identity: range2097891
display id: range2097891
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2
end: 1867
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097900
display id: annotation2097900
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097900/range2097900/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097900/range2097900/1
type: Range
persistent identity: range2097900
display id: range2097900
version: 1
direction: direction/forward
top level: BBa_K404001
start: 1883
end: 4213
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2097894
display id: annotation2097894
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097894/range2097894/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097894/range2097894/1
type: Range
persistent identity: range2097894
display id: range2097894
version: 1
direction: direction/forward
top level: BBa_K404001
start: 673
end: 1933
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097899
display id: annotation2097899
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097899/range2097899/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097899/range2097899/1
type: Range
persistent identity: range2097899
display id: range2097899
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2489
end: 4090
orientation: inline

BBa_K404157
type: ComponentDefinition
persistent identity: BBa K404157
display id: BBa_K404157
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_Long-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: HSPG-KO
sequence annotation: CMV
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: long-linker
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: ZEGFR_1907
sequence: https://synbiohub.org/public/igem/BBa_K404157_sequence/1

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101792
display id: annotation2101792
version: 1
top level: BBa_K404157
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2101792/range2101792/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2101792/range2101792/1
type: Range
persistent identity: range2101792
display id: range2101792
version: 1
direction: direction/forward
top level: BBa_K404157
start: 2240
end: 2242
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094994
display id: annotation2094994
version: 1
top level: BBa_K404157
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094994/range2094994/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094994/range2094994/1
type: Range
persistent identity: range2094994
display id: range2094994
version: 1
direction: direction/forward
top level: BBa_K404157
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101793
display id: annotation2101793
version: 1
top level: BBa_K404157
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2101793/range2101793/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2101793/range2101793/1
type: Range
persistent identity: range2101793
display id: range2101793
version: 1
direction: direction/forward
top level: BBa_K404157
start: 2231
end: 2233
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094997
display id: annotation2094997
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094997/range2094997/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094997/range2094997/1
type: Range
persistent identity: range2094997
display id: range2094997
version: 1
direction: direction/forward
top level: BBa_K404157
start: 893
end: 2686
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094998
display id: annotation2094998
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094998/range2094998/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094998/range2094998/1
type: Range
persistent identity: range2094998
display id: range2094998
version: 1
direction: direction/forward
top level: BBa_K404157
start: 1085
end: 2686
orientation: inline

long-linker
type: SequenceAnnotation
persistent identity: annotation2094996
display id: annotation2094996
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094996/range2094996/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094996/range2094996/1
type: Range
persistent identity: range2094996
display id: range2094996
version: 1
direction: direction/forward
top level: BBa_K404157
start: 851
end: 886
orientation: inline

AAV-2-cap (Stratagene)
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persistent identity: annotation2094999
display id: annotation2094999
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094999/range2094999/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094999/range2094999/1
type: Range
persistent identity: range2094999
display id: range2094999
version: 1
direction: direction/forward
top level: BBa_K404157
start: 893
end: 2809
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2094995
display id: annotation2094995
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094995/range2094995/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094995/range2094995/1
type: Range
persistent identity: range2094995
display id: range2094995
version: 1
direction: direction/forward
top level: BBa_K404157
start: 671
end: 844
orientation: inline

BBa_K404314
type: ComponentDefinition
persistent identity: BBa K404314
display id: BBa_K404314
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DARPin-E01
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: DARPin-E01
sequence: https://synbiohub.org/public/igem/BBa_K404314_sequence/1

DARPin-E01
type: SequenceAnnotation
persistent identity: annotation2094760
display id: annotation2094760
version: 1
top level: BBa_K404314
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404314/annotation2094760/range2094760/1

https://synbiohub.org/public/igem/BBa_K404314/annotation2094760/range2094760/1
type: Range
persistent identity: range2094760
display id: range2094760
version: 1
direction: direction/forward
top level: BBa_K404314
start: 1
end: 459
orientation: inline

BBa_K404227
type: ComponentDefinition
persistent identity: BBa K404227
display id: BBa_K404227
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587-RGD)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep40
sequence annotation: TG-spacer
sequence annotation: Rep52
sequence annotation: p5TATAless
sequence annotation: GLS-spacer
sequence annotation: VP1
sequence annotation: Rep68
sequence annotation: Rep78
sequence annotation: RGD
sequence annotation: VP3
sequence annotation: upstream 587
sequence annotation: downstream 587
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404227_sequence/1

Rep40
type: SequenceAnnotation
persistent identity: annotation2099010
display id: annotation2099010
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099010/range2099010/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099010/range2099010/1
type: Range
persistent identity: range2099010
display id: range2099010
version: 1
direction: direction/forward
top level: BBa_K404227
start: 673
end: 1933
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2099024
display id: annotation2099024
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099024/range2099024/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099024/range2099024/1
type: Range
persistent identity: range2099024
display id: range2099024
version: 1
direction: direction/forward
top level: BBa_K404227
start: 3644
end: 3649
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2099009
display id: annotation2099009
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099009/range2099009/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099009/range2099009/1
type: Range
persistent identity: range2099009
display id: range2099009
version: 1
direction: direction/forward
top level: BBa_K404227
start: 673
end: 1867
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2099021
display id: annotation2099021
version: 1
top level: BBa_K404227
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099021/range2099021/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099021/range2099021/1
type: Range
persistent identity: range2099021
display id: range2099021
version: 1
direction: direction/forward
top level: BBa_K404227
start: 4264
end: 4427
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2099028
display id: annotation2099028
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099028/range2099028/1

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VP1
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role: SO:0000110
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display id: range2099012
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Rep68
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Rep78
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top level: BBa_K404227
role: SO:0000110
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location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099007/range2099007/1

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type: Range
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display id: range2099007
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RGD
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role: SO:0000110
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type: Range
persistent identity: range2099023
display id: range2099023
version: 1
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VP3
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upstream 587
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downstream 587
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VP2
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role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2099018
display id: range2099018
version: 1
direction: direction/forward
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start: 2294
end: 4132
orientation: inline

BBa_K404012
type: ComponentDefinition
persistent identity: BBa K404012
display id: BBa_K404012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP3
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404012_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2109186
display id: annotation2109186
version: 1
top level: BBa_K404012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404012/annotation2109186/range2109186/1

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type: Range
persistent identity: range2109186
display id: range2109186
version: 1
direction: direction/forward
top level: BBa_K404012
start: 1
end: 1635
orientation: inline

BBa_K404204
type: ComponentDefinition
persistent identity: BBa K404204
display id: BBa_K404204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-453-Z34C
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Z34C
sequence annotation: upstream 453
sequence annotation: downstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404204_sequence/1

Z34C
type: SequenceAnnotation
persistent identity: annotation2078945
display id: annotation2078945
version: 1
top level: BBa_K404204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404204/annotation2078945/range2078945/1

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direction: direction/forward
top level: BBa_K404204
start: 43
end: 144
orientation: inline

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role: SO:0000110
role: feature/misc
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persistent identity: range2078944
display id: range2078944
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direction: direction/forward
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start: 1
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downstream 453
type: SequenceAnnotation
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display id: annotation2078946
version: 1
top level: BBa_K404204
role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2078946
display id: range2078946
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direction: direction/forward
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end: 165
orientation: inline

BBa_K404232
type: ComponentDefinition
persistent identity: BBa K404232
display id: BBa_K404232
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-RGD)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence annotation: GLS-spacer
sequence annotation: HSPG-KO
sequence annotation: VP3
sequence annotation: Rep68
sequence annotation: p5TATAless
sequence annotation: Rep40
sequence annotation: upstream 587
sequence annotation: downstream 587
sequence annotation: Rep78
sequence annotation: Rep52
sequence annotation: VP2
sequence annotation: TG-spacer
sequence annotation: RGD
sequence: https://synbiohub.org/public/igem/BBa_K404232_sequence/1

VP1
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persistent identity: annotation2098523
display id: annotation2098523
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098523/range2098523/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098523/range2098523/1
type: Range
persistent identity: range2098523
display id: range2098523
version: 1
direction: direction/forward
top level: BBa_K404232
start: 1883
end: 4132
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2098548
display id: annotation2098548
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098548/range2098548/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098548/range2098548/1
type: Range
persistent identity: range2098548
display id: range2098548
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3677
end: 3685
orientation: inline

HSPG-KO
type: SequenceAnnotation
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display id: annotation2101868
version: 1
top level: BBa_K404232
role: SO:0001059
role: feature/mutation
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type: Range
persistent identity: range2101868
display id: range2101868
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3635
end: 3637
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098526
display id: annotation2098526
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098526/range2098526/1

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type: Range
persistent identity: range2098526
display id: range2098526
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2489
end: 4132
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098520
display id: annotation2098520
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098520/range2098520/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098520/range2098520/1
type: Range
persistent identity: range2098520
display id: range2098520
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2
end: 1933
orientation: inline

p5TATAless
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persistent identity: annotation2098545
display id: annotation2098545
version: 1
top level: BBa_K404232
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098545/range2098545/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098545/range2098545/1
type: Range
persistent identity: range2098545
display id: range2098545
version: 1
direction: direction/forward
top level: BBa_K404232
start: 4264
end: 4427
orientation: inline

Rep40
type: SequenceAnnotation
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display id: annotation2098522
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098522/range2098522/1

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type: Range
persistent identity: range2098522
display id: range2098522
version: 1
direction: direction/forward
top level: BBa_K404232
start: 673
end: 1933
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2098549
display id: annotation2098549
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098549/range2098549/1

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persistent identity: range2098549
display id: range2098549
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3612
end: 3643
orientation: inline

HSPG-KO
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persistent identity: annotation2101867
display id: annotation2101867
version: 1
top level: BBa_K404232
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2101867/range2101867/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2101867/range2101867/1
type: Range
persistent identity: range2101867
display id: range2101867
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3678
end: 3680
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2098550
display id: annotation2098550
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098550/range2098550/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098550/range2098550/1
type: Range
persistent identity: range2098550
display id: range2098550
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3686
end: 3701
orientation: inline

Rep78
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display id: annotation2098519
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
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https://synbiohub.org/public/igem/BBa_K404232/annotation2098519/range2098519/1
type: Range
persistent identity: range2098519
display id: range2098519
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2
end: 1867
orientation: inline

Rep52
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persistent identity: annotation2098521
display id: annotation2098521
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098521/range2098521/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098521/range2098521/1
type: Range
persistent identity: range2098521
display id: range2098521
version: 1
direction: direction/forward
top level: BBa_K404232
start: 673
end: 1867
orientation: inline

VP2
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persistent identity: annotation2098525
display id: annotation2098525
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098525/range2098525/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098525/range2098525/1
type: Range
persistent identity: range2098525
display id: range2098525
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2294
end: 4132
orientation: inline

TG-spacer
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persistent identity: annotation2098547
display id: annotation2098547
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098547/range2098547/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098547/range2098547/1
type: Range
persistent identity: range2098547
display id: range2098547
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3644
end: 3649
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2098546
display id: annotation2098546
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098546/range2098546/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098546/range2098546/1
type: Range
persistent identity: range2098546
display id: range2098546
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3650
end: 3676
orientation: inline

BBa_K404222
type: ComponentDefinition
persistent identity: BBa K404222
display id: BBa_K404222
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-453-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep68
sequence annotation: AAV-a-cap (Stratagene)
sequence annotation: Rep78
sequence annotation: Rep52
sequence annotation: VP2
sequence annotation: Rep40
sequence annotation: VP1
sequence annotation: His-tag
sequence annotation: A-spacer
sequence annotation: p5TATAless
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404222_sequence/1

Rep68
type: SequenceAnnotation
persistent identity: annotation2098252
display id: annotation2098252
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098252/range2098252/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098252/range2098252/1
type: Range
persistent identity: range2098252
display id: range2098252
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2
end: 1933
orientation: inline

AAV-a-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2098259
display id: annotation2098259
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098259/range2098259/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098259/range2098259/1
type: Range
persistent identity: range2098259
display id: range2098259
version: 1
direction: direction/forward
top level: BBa_K404222
start: 1883
end: 4237
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098251
display id: annotation2098251
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098251/range2098251/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098251/range2098251/1
type: Range
persistent identity: range2098251
display id: range2098251
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2
end: 1867
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098254
display id: annotation2098254
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098254/range2098254/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098254/range2098254/1
type: Range
persistent identity: range2098254
display id: range2098254
version: 1
direction: direction/forward
top level: BBa_K404222
start: 673
end: 1867
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098256
display id: annotation2098256
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098256/range2098256/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098256/range2098256/1
type: Range
persistent identity: range2098256
display id: range2098256
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2294
end: 4114
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098253
display id: annotation2098253
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098253/range2098253/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098253/range2098253/1
type: Range
persistent identity: range2098253
display id: range2098253
version: 1
direction: direction/forward
top level: BBa_K404222
start: 673
end: 1933
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098255
display id: annotation2098255
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098255/range2098255/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098255/range2098255/1
type: Range
persistent identity: range2098255
display id: range2098255
version: 1
direction: direction/forward
top level: BBa_K404222
start: 1883
end: 4114
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2098260
display id: annotation2098260
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098260/range2098260/1

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type: Range
persistent identity: range2098260
display id: range2098260
version: 1
direction: direction/forward
top level: BBa_K404222
start: 3245
end: 3262
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098261
display id: annotation2098261
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098261/range2098261/1

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type: Range
persistent identity: range2098261
display id: range2098261
version: 1
direction: direction/forward
top level: BBa_K404222
start: 3242
end: 3244
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098258
display id: annotation2098258
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098258/range2098258/1

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type: Range
persistent identity: range2098258
display id: range2098258
version: 1
direction: direction/forward
top level: BBa_K404222
start: 4246
end: 4409
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098257
display id: annotation2098257
version: 1
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role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098257/range2098257/1

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type: Range
persistent identity: range2098257
display id: range2098257
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2489
end: 4114
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098262
display id: annotation2098262
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098262/range2098262/1

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type: Range
persistent identity: range2098262
display id: range2098262
version: 1
direction: direction/forward
top level: BBa_K404222
start: 3263
end: 3265
orientation: inline

BBa_K404159
type: ComponentDefinition
persistent identity: BBa K404159
display id: BBa_K404159
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_CFP_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CFP
sequence annotation: HSPG-KO
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence annotation: middle-linker
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404159_sequence/1

CFP
type: SequenceAnnotation
persistent identity: annotation2097915
display id: annotation2097915
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097915/range2097915/1

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type: Range
persistent identity: range2097915
display id: range2097915
version: 1
direction: direction/forward
top level: BBa_K404159
start: 662
end: 1385
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101798
display id: annotation2101798
version: 1
top level: BBa_K404159
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2101798/range2101798/1

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type: Range
persistent identity: range2101798
display id: range2101798
version: 1
direction: direction/forward
top level: BBa_K404159
start: 2759
end: 2761
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097918
display id: annotation2097918
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097918/range2097918/1

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type: Range
persistent identity: range2097918
display id: range2097918
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1613
end: 3214
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101797
display id: annotation2101797
version: 1
top level: BBa_K404159
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2101797/range2101797/1

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type: Range
persistent identity: range2101797
display id: range2101797
version: 1
direction: direction/forward
top level: BBa_K404159
start: 2768
end: 2770
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097919
display id: annotation2097919
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097919/range2097919/1

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type: Range
persistent identity: range2097919
display id: range2097919
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1421
end: 3337
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097917
display id: annotation2097917
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097917/range2097917/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097917/range2097917/1
type: Range
persistent identity: range2097917
display id: range2097917
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1421
end: 3214
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2097916
display id: annotation2097916
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097916/range2097916/1

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type: Range
persistent identity: range2097916
display id: range2097916
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1391
end: 1414
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097914
display id: annotation2097914
version: 1
top level: BBa_K404159
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097914/range2097914/1

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type: Range
persistent identity: range2097914
display id: range2097914
version: 1
direction: direction/forward
top level: BBa_K404159
start: 2
end: 655
orientation: inline

BBa_K404253
type: ComponentDefinition
persistent identity: BBa K404253
display id: BBa_K404253
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: GLS-spacer
sequence annotation: CMV
sequence annotation: TG-spacer
sequence annotation: HSPG-KO
sequence annotation: His-tag
sequence annotation: VP1
sequence annotation: downstream 587
sequence annotation: upstream 587
sequence annotation: VP2
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404253_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094657
display id: annotation2094657
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094657/range2094657/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094657/range2094657/1
type: Range
persistent identity: range2094657
display id: range2094657
version: 1
direction: direction/forward
top level: BBa_K404253
start: 671
end: 3031
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094660
display id: annotation2094660
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094660/range2094660/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094660/range2094660/1
type: Range
persistent identity: range2094660
display id: range2094660
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2453
end: 2461
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094653
display id: annotation2094653
version: 1
top level: BBa_K404253
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094653/range2094653/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094653/range2094653/1
type: Range
persistent identity: range2094653
display id: range2094653
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2
end: 655
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094659
display id: annotation2094659
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094659/range2094659/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094659/range2094659/1
type: Range
persistent identity: range2094659
display id: range2094659
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2429
end: 2434
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101880
display id: annotation2101880
version: 1
top level: BBa_K404253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2101880/range2101880/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2101880/range2101880/1
type: Range
persistent identity: range2101880
display id: range2101880
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2420
end: 2422
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2094658
display id: annotation2094658
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094658/range2094658/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094658/range2094658/1
type: Range
persistent identity: range2094658
display id: range2094658
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2435
end: 2452
orientation: inline

VP1
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persistent identity: annotation2094654
display id: annotation2094654
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
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https://synbiohub.org/public/igem/BBa_K404253/annotation2094654/range2094654/1
type: Range
persistent identity: range2094654
display id: range2094654
version: 1
direction: direction/forward
top level: BBa_K404253
start: 671
end: 2908
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094662
display id: annotation2094662
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094662/range2094662/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094662/range2094662/1
type: Range
persistent identity: range2094662
display id: range2094662
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2462
end: 2477
orientation: inline

HSPG-KO
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persistent identity: annotation2101879
display id: annotation2101879
version: 1
top level: BBa_K404253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2101879/range2101879/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2101879/range2101879/1
type: Range
persistent identity: range2101879
display id: range2101879
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2454
end: 2456
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094661
display id: annotation2094661
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094661/range2094661/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094661/range2094661/1
type: Range
persistent identity: range2094661
display id: range2094661
version: 1
direction: direction/forward
top level: BBa_K404253
start: 2397
end: 2428
orientation: inline

VP2
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display id: annotation2094655
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094655/range2094655/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094655/range2094655/1
type: Range
persistent identity: range2094655
display id: range2094655
version: 1
direction: direction/forward
top level: BBa_K404253
start: 1079
end: 2908
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094656
display id: annotation2094656
version: 1
top level: BBa_K404253
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404253/annotation2094656/range2094656/1

https://synbiohub.org/public/igem/BBa_K404253/annotation2094656/range2094656/1
type: Range
persistent identity: range2094656
display id: range2094656
version: 1
direction: direction/forward
top level: BBa_K404253
start: 1274
end: 2908
orientation: inline

BBa_K404233
type: ComponentDefinition
persistent identity: BBa K404233
display id: BBa_K404233
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-587KO-Z34C)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404215
type: ComponentDefinition
persistent identity: BBa K404215
display id: BBa_K404215
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-453-Empty
creator: Freiburg Bioware 2010
created: 2010-10-12T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404234
type: ComponentDefinition
persistent identity: BBa K404234
display id: BBa_K404234
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-Z34C-Spacer)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: p5TATAless
sequence annotation: VP3
sequence annotation: Rep78
sequence annotation: Rep40
sequence annotation: Rep68
sequence annotation: VP2
sequence annotation: TG-spacer
sequence annotation: Rep52
sequence annotation: downstream 587
sequence annotation: VP1
sequence annotation: HSPG-KO
sequence annotation: upstream 587
sequence annotation: Z34C
sequence annotation: GLS-spacer
sequence: https://synbiohub.org/public/igem/BBa_K404234_sequence/1

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098438
display id: annotation2098438
version: 1
top level: BBa_K404234
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098438/range2098438/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098438/range2098438/1
type: Range
persistent identity: range2098438
display id: range2098438
version: 1
direction: direction/forward
top level: BBa_K404234
start: 4339
end: 4502
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098437
display id: annotation2098437
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098437/range2098437/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098437/range2098437/1
type: Range
persistent identity: range2098437
display id: range2098437
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2489
end: 4207
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098430
display id: annotation2098430
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098430/range2098430/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098430/range2098430/1
type: Range
persistent identity: range2098430
display id: range2098430
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2
end: 1867
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098433
display id: annotation2098433
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098433/range2098433/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098433/range2098433/1
type: Range
persistent identity: range2098433
display id: range2098433
version: 1
direction: direction/forward
top level: BBa_K404234
start: 673
end: 1933
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098431
display id: annotation2098431
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098431/range2098431/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098431/range2098431/1
type: Range
persistent identity: range2098431
display id: range2098431
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2
end: 1933
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098436
display id: annotation2098436
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098436/range2098436/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098436/range2098436/1
type: Range
persistent identity: range2098436
display id: range2098436
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2294
end: 4207
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2098460
display id: annotation2098460
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098460/range2098460/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098460/range2098460/1
type: Range
persistent identity: range2098460
display id: range2098460
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3644
end: 3649
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098432
display id: annotation2098432
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098432/range2098432/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098432/range2098432/1
type: Range
persistent identity: range2098432
display id: range2098432
version: 1
direction: direction/forward
top level: BBa_K404234
start: 673
end: 1867
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2098500
display id: annotation2098500
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098500/range2098500/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098500/range2098500/1
type: Range
persistent identity: range2098500
display id: range2098500
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3761
end: 3776
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098434
display id: annotation2098434
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098434/range2098434/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098434/range2098434/1
type: Range
persistent identity: range2098434
display id: range2098434
version: 1
direction: direction/forward
top level: BBa_K404234
start: 1883
end: 4207
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101869
display id: annotation2101869
version: 1
top level: BBa_K404234
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2101869/range2101869/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2101869/range2101869/1
type: Range
persistent identity: range2101869
display id: range2101869
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3753
end: 3755
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2098435
display id: annotation2098435
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098435/range2098435/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098435/range2098435/1
type: Range
persistent identity: range2098435
display id: range2098435
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3612
end: 3643
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101870
display id: annotation2101870
version: 1
top level: BBa_K404234
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2101870/range2101870/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2101870/range2101870/1
type: Range
persistent identity: range2101870
display id: range2101870
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3648
end: 3650
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2098439
display id: annotation2098439
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098439/range2098439/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098439/range2098439/1
type: Range
persistent identity: range2098439
display id: range2098439
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3650
end: 3751
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2098479
display id: annotation2098479
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098479/range2098479/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098479/range2098479/1
type: Range
persistent identity: range2098479
display id: range2098479
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3752
end: 3760
orientation: inline

BBa_K404224
type: ComponentDefinition
persistent identity: BBa K404224
display id: BBa_K404224
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-453-Z34C)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: p5 TATAless
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: Rep 78
sequence annotation: Rep 52
sequence annotation: Z34C
sequence annotation: Rep 68
sequence annotation: AAV-2 cap (Stratagene)
sequence annotation: Rep 40
sequence: https://synbiohub.org/public/igem/BBa_K404224_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2098458
display id: annotation2098458
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098458/range2098458/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098458/range2098458/1
type: Range
persistent identity: range2098458
display id: range2098458
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2294
end: 4192
orientation: inline

p5 TATAless
type: SequenceAnnotation
persistent identity: annotation2098845
display id: annotation2098845
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098845/range2098845/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098845/range2098845/1
type: Range
persistent identity: range2098845
display id: range2098845
version: 1
direction: direction/forward
top level: BBa_K404224
start: 4324
end: 4487
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098551
display id: annotation2098551
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098551/range2098551/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098551/range2098551/1
type: Range
persistent identity: range2098551
display id: range2098551
version: 1
direction: direction/forward
top level: BBa_K404224
start: 1883
end: 4192
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098524
display id: annotation2098524
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098524/range2098524/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098524/range2098524/1
type: Range
persistent identity: range2098524
display id: range2098524
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2489
end: 4192
orientation: inline

Rep 78
type: SequenceAnnotation
persistent identity: annotation2098422
display id: annotation2098422
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098422/range2098422/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098422/range2098422/1
type: Range
persistent identity: range2098422
display id: range2098422
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2
end: 1867
orientation: inline

Rep 52
type: SequenceAnnotation
persistent identity: annotation2098427
display id: annotation2098427
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098427/range2098427/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098427/range2098427/1
type: Range
persistent identity: range2098427
display id: range2098427
version: 1
direction: direction/forward
top level: BBa_K404224
start: 673
end: 1867
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2098940
display id: annotation2098940
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098940/range2098940/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098940/range2098940/1
type: Range
persistent identity: range2098940
display id: range2098940
version: 1
direction: direction/forward
top level: BBa_K404224
start: 3242
end: 3343
orientation: inline

Rep 68
type: SequenceAnnotation
persistent identity: annotation2098424
display id: annotation2098424
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098424/range2098424/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098424/range2098424/1
type: Range
persistent identity: range2098424
display id: range2098424
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2
end: 1933
orientation: inline

AAV-2 cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2098661
display id: annotation2098661
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098661/range2098661/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098661/range2098661/1
type: Range
persistent identity: range2098661
display id: range2098661
version: 1
direction: direction/forward
top level: BBa_K404224
start: 1883
end: 3795
orientation: inline

Rep 40
type: SequenceAnnotation
persistent identity: annotation2098428
display id: annotation2098428
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098428/range2098428/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098428/range2098428/1
type: Range
persistent identity: range2098428
display id: range2098428
version: 1
direction: direction/forward
top level: BBa_K404224
start: 673
end: 1933
orientation: inline

BBa_K404007
type: ComponentDefinition
persistent identity: BBa K404007
display id: BBa_K404007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: BBa_I712004
sequence annotation: VP1
sequence annotation: VP2
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404007_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2099427
display id: annotation2099427
version: 1
top level: BBa_K404007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099427/range2099427/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099427/range2099427/1
type: Range
persistent identity: range2099427
display id: range2099427
version: 1
direction: direction/forward
top level: BBa_K404007
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2099432
display id: annotation2099432
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099432/range2099432/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099432/range2099432/1
type: Range
persistent identity: range2099432
display id: range2099432
version: 1
direction: direction/forward
top level: BBa_K404007
start: 1274
end: 2875
orientation: inline

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation2099428
display id: annotation2099428
version: 1
top level: BBa_K404007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099428/range2099428/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099428/range2099428/1
type: Range
persistent identity: range2099428
display id: range2099428
version: 1
direction: direction/forward
top level: BBa_K404007
start: 2
end: 655
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2099429
display id: annotation2099429
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099429/range2099429/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099429/range2099429/1
type: Range
persistent identity: range2099429
display id: range2099429
version: 1
direction: direction/forward
top level: BBa_K404007
start: 671
end: 2875
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2099431
display id: annotation2099431
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099431/range2099431/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099431/range2099431/1
type: Range
persistent identity: range2099431
display id: range2099431
version: 1
direction: direction/forward
top level: BBa_K404007
start: 1079
end: 2875
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2099430
display id: annotation2099430
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099430/range2099430/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099430/range2099430/1
type: Range
persistent identity: range2099430
display id: range2099430
version: 1
direction: direction/forward
top level: BBa_K404007
start: 671
end: 2998
orientation: inline

BBa_K404002
type: ComponentDefinition
persistent identity: BBa K404002
display id: BBa_K404002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep40
sequence annotation: VP2
sequence annotation: Rep68
sequence annotation: p5TATAless
sequence annotation: VP1
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP3
sequence annotation: Rep78
sequence annotation: Rep52
sequence: https://synbiohub.org/public/igem/BBa_K404002_sequence/1

Rep40
type: SequenceAnnotation
persistent identity: annotation2097809
display id: annotation2097809
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097809/range2097809/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097809/range2097809/1
type: Range
persistent identity: range2097809
display id: range2097809
version: 1
direction: direction/forward
top level: BBa_K404002
start: 671
end: 1931
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097812
display id: annotation2097812
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097812/range2097812/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097812/range2097812/1
type: Range
persistent identity: range2097812
display id: range2097812
version: 1
direction: direction/forward
top level: BBa_K404002
start: 2292
end: 4088
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2097808
display id: annotation2097808
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097808/range2097808/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097808/range2097808/1
type: Range
persistent identity: range2097808
display id: range2097808
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1
end: 1931
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2097816
display id: annotation2097816
version: 1
top level: BBa_K404002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097816/range2097816/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097816/range2097816/1
type: Range
persistent identity: range2097816
display id: range2097816
version: 1
direction: direction/forward
top level: BBa_K404002
start: 4220
end: 4383
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2097811
display id: annotation2097811
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097811/range2097811/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097811/range2097811/1
type: Range
persistent identity: range2097811
display id: range2097811
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1881
end: 4088
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097814
display id: annotation2097814
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097814/range2097814/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097814/range2097814/1
type: Range
persistent identity: range2097814
display id: range2097814
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1881
end: 4212
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097813
display id: annotation2097813
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097813/range2097813/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097813/range2097813/1
type: Range
persistent identity: range2097813
display id: range2097813
version: 1
direction: direction/forward
top level: BBa_K404002
start: 2487
end: 4088
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2097807
display id: annotation2097807
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097807/range2097807/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097807/range2097807/1
type: Range
persistent identity: range2097807
display id: range2097807
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1
end: 1865
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2097810
display id: annotation2097810
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097810/range2097810/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097810/range2097810/1
type: Range
persistent identity: range2097810
display id: range2097810
version: 1
direction: direction/forward
top level: BBa_K404002
start: 671
end: 1865
orientation: inline

BBa_K404247
type: ComponentDefinition
persistent identity: BBa K404247
display id: BBa_K404247
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence annotation: CMV promoter
sequence annotation: TG-spacer
sequence annotation: upstream 587
sequence annotation: downstream 587
sequence annotation: VP3
sequence annotation: VP1
sequence annotation: His-tag
sequence annotation: GLS-spacer
sequence: https://synbiohub.org/public/igem/BBa_K404247_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094290
display id: annotation2094290
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094290/range2094290/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094290/range2094290/1
type: Range
persistent identity: range2094290
display id: range2094290
version: 1
direction: direction/forward
top level: BBa_K404247
start: 671
end: 3031
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094282
display id: annotation2094282
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094282/range2094282/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094282/range2094282/1
type: Range
persistent identity: range2094282
display id: range2094282
version: 1
direction: direction/forward
top level: BBa_K404247
start: 1079
end: 2908
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094280
display id: annotation2094280
version: 1
top level: BBa_K404247
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094280/range2094280/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094280/range2094280/1
type: Range
persistent identity: range2094280
display id: range2094280
version: 1
direction: direction/forward
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TG-spacer
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upstream 587
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downstream 587
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role: SO:0000110
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VP3
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VP1
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His-tag
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GLS-spacer
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BBa_K404005
type: ComponentDefinition
persistent identity: BBa K404005
display id: BBa_K404005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP23(ViralBrick-587KO-empty)_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep40
sequence annotation: p5TATAless
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: HSPG-KO
sequence annotation: Rep78
sequence annotation: Rep68
sequence annotation: Rep52
sequence: https://synbiohub.org/public/igem/BBa_K404005_sequence/1

Rep40
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p5TATAless
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orientation: inline

VP3
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persistent identity: range2097889
display id: range2097889
version: 1
direction: direction/forward
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end: 4090
orientation: inline

VP2
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display id: annotation2097888
version: 1
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role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2097888
display id: range2097888
version: 1
direction: direction/forward
top level: BBa_K404005
start: 2294
end: 4090
orientation: inline

HSPG-KO
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display id: annotation2101713
version: 1
top level: BBa_K404005
role: SO:0001059
role: feature/mutation
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type: Range
persistent identity: range2101713
display id: range2101713
version: 1
direction: direction/forward
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start: 3644
end: 3646
orientation: inline

Rep78
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display id: annotation2097885
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role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2097885
display id: range2097885
version: 1
direction: direction/forward
top level: BBa_K404005
start: 1
end: 1933
orientation: inline

HSPG-KO
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display id: annotation2101714
version: 1
top level: BBa_K404005
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2101714/range2101714/1

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persistent identity: range2101714
display id: range2101714
version: 1
direction: direction/forward
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start: 3635
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orientation: inline

Rep68
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display id: annotation2097884
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role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2097884
display id: range2097884
version: 1
direction: direction/forward
top level: BBa_K404005
start: 1
end: 1867
orientation: inline

Rep52
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display id: annotation2097887
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097887/range2097887/1

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type: Range
persistent identity: range2097887
display id: range2097887
version: 1
direction: direction/forward
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start: 673
end: 1867
orientation: inline

BBa_K404011
type: ComponentDefinition
persistent identity: BBa K404011
display id: BBa_K404011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP2
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: CMV
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VP2
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display id: annotation2097904
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persistent identity: range2097904
display id: range2097904
version: 1
direction: direction/forward
top level: BBa_K404011
start: 670
end: 2500
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097903
display id: annotation2097903
version: 1
top level: BBa_K404011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404011/annotation2097903/range2097903/1

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type: Range
persistent identity: range2097903
display id: range2097903
version: 1
direction: direction/forward
top level: BBa_K404011
start: 2
end: 655
orientation: inline

BBa_K404235
type: ComponentDefinition
persistent identity: BBa K404235
display id: BBa_K404235
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]Rep-VP123_p5TATAless (ViralBrick 453 Z34C-587 HSPG-KO)
creator: Freiburg Bioware 2010
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: HSPG-KO
sequence annotation: Rep68
sequence annotation: Rep78
sequence annotation: Rep40
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP1
sequence annotation: p5TATAless
sequence annotation: VP2
sequence annotation: Z34C
sequence annotation: Rep52
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404235_sequence/1

HSPG-KO
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persistent identity: annotation2101871
display id: annotation2101871
version: 1
top level: BBa_K404235
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2101871/range2101871/1

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type: Range
persistent identity: range2101871
display id: range2101871
version: 1
direction: direction/forward
top level: BBa_K404235
start: 3377
end: 3379
orientation: inline

HSPG-KO
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persistent identity: annotation2101872
display id: annotation2101872
version: 1
top level: BBa_K404235
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2101872/range2101872/1

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type: Range
persistent identity: range2101872
display id: range2101872
version: 1
direction: direction/forward
top level: BBa_K404235
start: 3155
end: 3157
orientation: inline

Rep68
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display id: annotation2098415
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098415/range2098415/1

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type: Range
persistent identity: range2098415
display id: range2098415
version: 1
direction: direction/forward
top level: BBa_K404235
start: 2
end: 1933
orientation: inline

Rep78
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display id: annotation2098414
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2098414
display id: range2098414
version: 1
direction: direction/forward
top level: BBa_K404235
start: 2
end: 1867
orientation: inline

Rep40
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persistent identity: annotation2098417
display id: annotation2098417
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098417/range2098417/1

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type: Range
persistent identity: range2098417
display id: range2098417
version: 1
direction: direction/forward
top level: BBa_K404235
start: 673
end: 1933
orientation: inline

AAV-2-cap (Stratagene)
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persistent identity: annotation2098425
display id: annotation2098425
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098425/range2098425/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098425/range2098425/1
type: Range
persistent identity: range2098425
display id: range2098425
version: 1
direction: direction/forward
top level: BBa_K404235
start: 1833
end: 4315
orientation: inline

VP1
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persistent identity: annotation2098418
display id: annotation2098418
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098418/range2098418/1

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type: Range
persistent identity: range2098418
display id: range2098418
version: 1
direction: direction/forward
top level: BBa_K404235
start: 1833
end: 4192
orientation: inline

p5TATAless
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persistent identity: annotation2098423
display id: annotation2098423
version: 1
top level: BBa_K404235
role: SO:0000167
role: feature/promoter
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type: Range
persistent identity: range2098423
display id: range2098423
version: 1
direction: direction/forward
top level: BBa_K404235
start: 4324
end: 4487
orientation: inline

VP2
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persistent identity: annotation2098419
display id: annotation2098419
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098419/range2098419/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098419/range2098419/1
type: Range
persistent identity: range2098419
display id: range2098419
version: 1
direction: direction/forward
top level: BBa_K404235
start: 2294
end: 4192
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2098421
display id: annotation2098421
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098421/range2098421/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098421/range2098421/1
type: Range
persistent identity: range2098421
display id: range2098421
version: 1
direction: direction/forward
top level: BBa_K404235
start: 3242
end: 3343
orientation: inline

Rep52
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display id: annotation2098416
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098416/range2098416/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098416/range2098416/1
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persistent identity: range2098416
display id: range2098416
version: 1
direction: direction/forward
top level: BBa_K404235
start: 673
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orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098420
display id: annotation2098420
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098420/range2098420/1

https://synbiohub.org/public/igem/BBa_K404235/annotation2098420/range2098420/1
type: Range
persistent identity: range2098420
display id: range2098420
version: 1
direction: direction/forward
top level: BBa_K404235
start: 2489
end: 4192
orientation: inline

BBa_K404167
type: ComponentDefinition
persistent identity: BBa K404167
display id: BBa_K404167
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_VP1up_NLS_mVenus_VP23(ViralBrick-587-KO-BAP)
creator: Freiburg Bioware 2010
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: HSPG-KO
sequence annotation: TG-spacer
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: BAP
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: NLS
sequence annotation: VP1up
sequence annotation: mVenus
sequence annotation: upstream 587
sequence annotation: GLS-spacer
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404167_sequence/1

VP2
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persistent identity: annotation2095314
display id: annotation2095314
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095314/range2095314/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095314/range2095314/1
type: Range
persistent identity: range2095314
display id: range2095314
version: 1
direction: direction/forward
top level: BBa_K404167
start: 1835
end: 3688
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101842
display id: annotation2101842
version: 1
top level: BBa_K404167
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2101842/range2101842/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2101842/range2101842/1
type: Range
persistent identity: range2101842
display id: range2101842
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3234
end: 3236
orientation: inline

TG-spacer
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persistent identity: annotation2095318
display id: annotation2095318
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095318/range2095318/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095318/range2095318/1
type: Range
persistent identity: range2095318
display id: range2095318
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3182
end: 3187
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095316
display id: annotation2095316
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095316/range2095316/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095316/range2095316/1
type: Range
persistent identity: range2095316
display id: range2095316
version: 1
direction: direction/forward
top level: BBa_K404167
start: 1835
end: 3811
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2095317
display id: annotation2095317
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095317/range2095317/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095317/range2095317/1
type: Range
persistent identity: range2095317
display id: range2095317
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3188
end: 3232
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095311
display id: annotation2095311
version: 1
top level: BBa_K404167
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095311/range2095311/1

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type: Range
persistent identity: range2095311
display id: range2095311
version: 1
direction: direction/forward
top level: BBa_K404167
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095315
display id: annotation2095315
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095315/range2095315/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095315/range2095315/1
type: Range
persistent identity: range2095315
display id: range2095315
version: 1
direction: direction/forward
top level: BBa_K404167
start: 2027
end: 3688
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2095310
display id: annotation2095310
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095310/range2095310/1

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type: Range
persistent identity: range2095310
display id: range2095310
version: 1
direction: direction/forward
top level: BBa_K404167
start: 1088
end: 1108
orientation: inline

VP1up
type: SequenceAnnotation
persistent identity: annotation2095312
display id: annotation2095312
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095312/range2095312/1

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type: Range
persistent identity: range2095312
display id: range2095312
version: 1
direction: direction/forward
top level: BBa_K404167
start: 673
end: 1081
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2095313
display id: annotation2095313
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095313/range2095313/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095313/range2095313/1
type: Range
persistent identity: range2095313
display id: range2095313
version: 1
direction: direction/forward
top level: BBa_K404167
start: 1115
end: 1828
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101843
display id: annotation2101843
version: 1
top level: BBa_K404167
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2101843/range2101843/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2101843/range2101843/1
type: Range
persistent identity: range2101843
display id: range2101843
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3172
end: 3174
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2095321
display id: annotation2095321
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095321/range2095321/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095321/range2095321/1
type: Range
persistent identity: range2095321
display id: range2095321
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3150
end: 3181
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2095319
display id: annotation2095319
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095319/range2095319/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095319/range2095319/1
type: Range
persistent identity: range2095319
display id: range2095319
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3233
end: 3241
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2095320
display id: annotation2095320
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095320/range2095320/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095320/range2095320/1
type: Range
persistent identity: range2095320
display id: range2095320
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3242
end: 3257
orientation: inline

BBa_K404231
type: ComponentDefinition
persistent identity: BBa K404231
display id: BBa_K404231
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-587KO-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404221
type: ComponentDefinition
persistent identity: BBa K404221
display id: BBa_K404221
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-453-BAP)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: rep78
sequence annotation: rep68
sequence annotation: rep40
sequence annotation: rep52
sequence: https://synbiohub.org/public/igem/BBa_K404221_sequence/1

rep78
type: SequenceAnnotation
persistent identity: annotation2095283
display id: annotation2095283
version: 1
top level: BBa_K404221
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K404221/annotation2095283/range2095283/1

https://synbiohub.org/public/igem/BBa_K404221/annotation2095283/range2095283/1
type: Range
persistent identity: range2095283
display id: range2095283
version: 1
direction: direction/forward
top level: BBa_K404221
start: 6
end: 1865
orientation: inline

rep68
type: SequenceAnnotation
persistent identity: annotation2095280
display id: annotation2095280
version: 1
top level: BBa_K404221
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K404221/annotation2095280/range2095280/1

https://synbiohub.org/public/igem/BBa_K404221/annotation2095280/range2095280/1
type: Range
persistent identity: range2095280
display id: range2095280
version: 1
direction: direction/forward
top level: BBa_K404221
start: 6
end: 1931
orientation: inline

rep40
type: SequenceAnnotation
persistent identity: annotation2095289
display id: annotation2095289
version: 1
top level: BBa_K404221
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K404221/annotation2095289/range2095289/1

https://synbiohub.org/public/igem/BBa_K404221/annotation2095289/range2095289/1
type: Range
persistent identity: range2095289
display id: range2095289
version: 1
direction: direction/forward
top level: BBa_K404221
start: 672
end: 1931
orientation: inline

rep52
type: SequenceAnnotation
persistent identity: annotation2095291
display id: annotation2095291
version: 1
top level: BBa_K404221
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K404221/annotation2095291/range2095291/1

https://synbiohub.org/public/igem/BBa_K404221/annotation2095291/range2095291/1
type: Range
persistent identity: range2095291
display id: range2095291
version: 1
direction: direction/forward
top level: BBa_K404221
start: 672
end: 1865
orientation: inline

BBa_K404242
type: ComponentDefinition
persistent identity: BBa K404242
display id: BBa_K404242
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]VP123 (ViralBrick-453-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-09-19T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: His Tag
sequence annotation: AAP
sequence annotation: VP2
sequence annotation: CMV
sequence annotation: downstream 453
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP1
sequence annotation: upstrem 453
sequence: https://synbiohub.org/public/igem/BBa_K404242_sequence/1

His Tag
type: SequenceAnnotation
persistent identity: annotation2094176
display id: annotation2094176
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094176/range2094176/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094176/range2094176/1
type: Range
persistent identity: range2094176
display id: range2094176
version: 1
direction: direction/forward
top level: BBa_K404242
start: 2030
end: 2047
orientation: inline

AAP
type: SequenceAnnotation
persistent identity: annotation2094205
display id: annotation2094205
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094205/range2094205/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094205/range2094205/1
type: Range
persistent identity: range2094205
display id: range2094205
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1194
end: 1808
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094172
display id: annotation2094172
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094172/range2094172/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094172/range2094172/1
type: Range
persistent identity: range2094172
display id: range2094172
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1079
end: 2899
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094170
display id: annotation2094170
version: 1
top level: BBa_K404242
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094170/range2094170/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094170/range2094170/1
type: Range
persistent identity: range2094170
display id: range2094170
version: 1
direction: direction/forward
top level: BBa_K404242
start: 2
end: 655
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2094177
display id: annotation2094177
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094177/range2094177/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094177/range2094177/1
type: Range
persistent identity: range2094177
display id: range2094177
version: 1
direction: direction/forward
top level: BBa_K404242
start: 2051
end: 2064
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094173
display id: annotation2094173
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094173/range2094173/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094173/range2094173/1
type: Range
persistent identity: range2094173
display id: range2094173
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1274
end: 2899
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094174
display id: annotation2094174
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094174/range2094174/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094174/range2094174/1
type: Range
persistent identity: range2094174
display id: range2094174
version: 1
direction: direction/forward
top level: BBa_K404242
start: 671
end: 3022
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094171
display id: annotation2094171
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094171/range2094171/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094171/range2094171/1
type: Range
persistent identity: range2094171
display id: range2094171
version: 1
direction: direction/forward
top level: BBa_K404242
start: 671
end: 2899
orientation: inline

upstrem 453
type: SequenceAnnotation
persistent identity: annotation2094175
display id: annotation2094175
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094175/range2094175/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094175/range2094175/1
type: Range
persistent identity: range2094175
display id: range2094175
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1989
end: 2026
orientation: inline

BBa_K404225
type: ComponentDefinition
persistent identity: BBa K404225
display id: BBa_K404225
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587-BAP)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP 1
sequence annotation: PLA2
sequence annotation: p5 TATAless
sequence annotation: Rep 68
sequence annotation: Rep78
sequence annotation: Rep 40
sequence annotation: TG-Spacer
sequence annotation: BAP
sequence annotation: GLS-Spacer
sequence annotation: Rep 52
sequence annotation: AAV-2 cap Stratagene
sequence annotation: VP 2
sequence annotation: VP 3
sequence: https://synbiohub.org/public/igem/BBa_K404225_sequence/1

VP 1
type: SequenceAnnotation
persistent identity: annotation2099011
display id: annotation2099011
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099011/range2099011/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099011/range2099011/1
type: Range
persistent identity: range2099011
display id: range2099011
version: 1
direction: direction/forward
top level: BBa_K404225
start: 1883
end: 4150
orientation: inline

PLA2
type: SequenceAnnotation
persistent identity: annotation2099005
display id: annotation2099005
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099005/range2099005/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099005/range2099005/1
type: Range
persistent identity: range2099005
display id: range2099005
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2015
end: 2191
orientation: inline

p5 TATAless
type: SequenceAnnotation
persistent identity: annotation2099022
display id: annotation2099022
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099022/range2099022/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099022/range2099022/1
type: Range
persistent identity: range2099022
display id: range2099022
version: 1
direction: direction/forward
top level: BBa_K404225
start: 4282
end: 4445
orientation: inline

Rep 68
type: SequenceAnnotation
persistent identity: annotation2098997
display id: annotation2098997
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2098997/range2098997/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2098997/range2098997/1
type: Range
persistent identity: range2098997
display id: range2098997
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2
end: 1933
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098996
display id: annotation2098996
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2098996/range2098996/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2098996/range2098996/1
type: Range
persistent identity: range2098996
display id: range2098996
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2
end: 1867
orientation: inline

Rep 40
type: SequenceAnnotation
persistent identity: annotation2099004
display id: annotation2099004
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099004/range2099004/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099004/range2099004/1
type: Range
persistent identity: range2099004
display id: range2099004
version: 1
direction: direction/forward
top level: BBa_K404225
start: 673
end: 1933
orientation: inline

TG-Spacer
type: SequenceAnnotation
persistent identity: annotation2099026
display id: annotation2099026
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099026/range2099026/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099026/range2099026/1
type: Range
persistent identity: range2099026
display id: range2099026
version: 1
direction: direction/forward
top level: BBa_K404225
start: 3644
end: 3649
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2099025
display id: annotation2099025
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099025/range2099025/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099025/range2099025/1
type: Range
persistent identity: range2099025
display id: range2099025
version: 1
direction: direction/forward
top level: BBa_K404225
start: 3650
end: 3694
orientation: inline

GLS-Spacer
type: SequenceAnnotation
persistent identity: annotation2099027
display id: annotation2099027
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099027/range2099027/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099027/range2099027/1
type: Range
persistent identity: range2099027
display id: range2099027
version: 1
direction: direction/forward
top level: BBa_K404225
start: 3695
end: 3703
orientation: inline

Rep 52
type: SequenceAnnotation
persistent identity: annotation2099003
display id: annotation2099003
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099003/range2099003/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099003/range2099003/1
type: Range
persistent identity: range2099003
display id: range2099003
version: 1
direction: direction/forward
top level: BBa_K404225
start: 673
end: 1867
orientation: inline

AAV-2 cap Stratagene
type: SequenceAnnotation
persistent identity: annotation2099006
display id: annotation2099006
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099006/range2099006/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099006/range2099006/1
type: Range
persistent identity: range2099006
display id: range2099006
version: 1
direction: direction/forward
top level: BBa_K404225
start: 1883
end: 3753
orientation: inline

VP 2
type: SequenceAnnotation
persistent identity: annotation2099017
display id: annotation2099017
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099017/range2099017/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099017/range2099017/1
type: Range
persistent identity: range2099017
display id: range2099017
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2294
end: 4150
orientation: inline

VP 3
type: SequenceAnnotation
persistent identity: annotation2099020
display id: annotation2099020
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099020/range2099020/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099020/range2099020/1
type: Range
persistent identity: range2099020
display id: range2099020
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2489
end: 4150
orientation: inline

BBa_K404162
type: ComponentDefinition
persistent identity: BBa K404162
display id: BBa_K404162
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-BAP)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: middle-linker
sequence annotation: HSPG-KO
sequence annotation: BAP
sequence annotation: GLS-spacer
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: CMV
sequence annotation: ZEGFR_1907
sequence annotation: upstream 587
sequence annotation: TG-spacer
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404162_sequence/1

middle-linker
type: SequenceAnnotation
persistent identity: annotation2095014
display id: annotation2095014
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095014/range2095014/1

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type: Range
persistent identity: range2095014
display id: range2095014
version: 1
direction: direction/forward
top level: BBa_K404162
start: 851
end: 874
orientation: inline

HSPG-KO
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persistent identity: annotation2101812
display id: annotation2101812
version: 1
top level: BBa_K404162
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2101812/range2101812/1

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type: Range
persistent identity: range2101812
display id: range2101812
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2219
end: 2221
orientation: inline

BAP
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persistent identity: annotation2095018
display id: annotation2095018
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095018/range2095018/1

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type: Range
persistent identity: range2095018
display id: range2095018
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2234
end: 2278
orientation: inline

GLS-spacer
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persistent identity: annotation2095020
display id: annotation2095020
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095020/range2095020/1

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type: Range
persistent identity: range2095020
display id: range2095020
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2279
end: 2287
orientation: inline

VP3
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display id: annotation2095016
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095016/range2095016/1

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type: Range
persistent identity: range2095016
display id: range2095016
version: 1
direction: direction/forward
top level: BBa_K404162
start: 1073
end: 2734
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095015
display id: annotation2095015
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095015/range2095015/1

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type: Range
persistent identity: range2095015
display id: range2095015
version: 1
direction: direction/forward
top level: BBa_K404162
start: 881
end: 2734
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095012
display id: annotation2095012
version: 1
top level: BBa_K404162
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095012/range2095012/1

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type: Range
persistent identity: range2095012
display id: range2095012
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2
end: 655
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2095013
display id: annotation2095013
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095013/range2095013/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095013/range2095013/1
type: Range
persistent identity: range2095013
display id: range2095013
version: 1
direction: direction/forward
top level: BBa_K404162
start: 671
end: 844
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2095022
display id: annotation2095022
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095022/range2095022/1

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type: Range
persistent identity: range2095022
display id: range2095022
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2196
end: 2227
orientation: inline

TG-spacer
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persistent identity: annotation2095019
display id: annotation2095019
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095019/range2095019/1

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type: Range
persistent identity: range2095019
display id: range2095019
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2228
end: 2233
orientation: inline

AAV-2-cap (Stratagene)
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display id: annotation2095017
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095017/range2095017/1

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persistent identity: range2095017
display id: range2095017
version: 1
direction: direction/forward
top level: BBa_K404162
start: 881
end: 2857
orientation: inline

downstream 587
type: SequenceAnnotation
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display id: annotation2095021
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095021/range2095021/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095021/range2095021/1
type: Range
persistent identity: range2095021
display id: range2095021
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2288
end: 2303
orientation: inline

HSPG-KO
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display id: annotation2101813
version: 1
top level: BBa_K404162
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2101813/range2101813/1

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type: Range
persistent identity: range2101813
display id: range2101813
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2288
end: 2290
orientation: inline

BBa_K404216
type: ComponentDefinition
persistent identity: BBa K404216
display id: BBa_K404216
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587-Empty
creator: Freiburg Bioware 2010
created: 2010-10-12T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404229
type: ComponentDefinition
persistent identity: BBa K404229
display id: BBa_K404229
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-BAP)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep68
sequence annotation: downstream 587
sequence annotation: p5TATAless
sequence annotation: HSPG-KO
sequence annotation: upstream 587
sequence annotation: VP2
sequence annotation: Rep52
sequence annotation: BAP
sequence annotation: Rep40
sequence annotation: GLS-spacer
sequence annotation: VP3
sequence annotation: VP1
sequence annotation: Rep78
sequence annotation: TG-spacer
sequence: https://synbiohub.org/public/igem/BBa_K404229_sequence/1

Rep68
type: SequenceAnnotation
persistent identity: annotation2098608
display id: annotation2098608
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098608/range2098608/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098608/range2098608/1
type: Range
persistent identity: range2098608
display id: range2098608
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2
end: 1933
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2098995
display id: annotation2098995
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098995/range2098995/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098995/range2098995/1
type: Range
persistent identity: range2098995
display id: range2098995
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3704
end: 3719
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098912
display id: annotation2098912
version: 1
top level: BBa_K404229
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098912/range2098912/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098912/range2098912/1
type: Range
persistent identity: range2098912
display id: range2098912
version: 1
direction: direction/forward
top level: BBa_K404229
start: 4282
end: 4445
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101846
display id: annotation2101846
version: 1
top level: BBa_K404229
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2101846/range2101846/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2101846/range2101846/1
type: Range
persistent identity: range2101846
display id: range2101846
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3696
end: 3698
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2098994
display id: annotation2098994
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098994/range2098994/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098994/range2098994/1
type: Range
persistent identity: range2098994
display id: range2098994
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3612
end: 3643
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098811
display id: annotation2098811
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098811/range2098811/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098811/range2098811/1
type: Range
persistent identity: range2098811
display id: range2098811
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2294
end: 4150
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098660
display id: annotation2098660
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098660/range2098660/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098660/range2098660/1
type: Range
persistent identity: range2098660
display id: range2098660
version: 1
direction: direction/forward
top level: BBa_K404229
start: 673
end: 1867
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2098961
display id: annotation2098961
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098961/range2098961/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098961/range2098961/1
type: Range
persistent identity: range2098961
display id: range2098961
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3650
end: 3694
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098709
display id: annotation2098709
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098709/range2098709/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098709/range2098709/1
type: Range
persistent identity: range2098709
display id: range2098709
version: 1
direction: direction/forward
top level: BBa_K404229
start: 673
end: 1933
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2098993
display id: annotation2098993
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098993/range2098993/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098993/range2098993/1
type: Range
persistent identity: range2098993
display id: range2098993
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3695
end: 3703
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098861
display id: annotation2098861
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098861/range2098861/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098861/range2098861/1
type: Range
persistent identity: range2098861
display id: range2098861
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2489
end: 4150
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101847
display id: annotation2101847
version: 1
top level: BBa_K404229
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2101847/range2101847/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2101847/range2101847/1
type: Range
persistent identity: range2101847
display id: range2101847
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3635
end: 3637
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098773
display id: annotation2098773
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098773/range2098773/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098773/range2098773/1
type: Range
persistent identity: range2098773
display id: range2098773
version: 1
direction: direction/forward
top level: BBa_K404229
start: 1883
end: 4150
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098552
display id: annotation2098552
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098552/range2098552/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098552/range2098552/1
type: Range
persistent identity: range2098552
display id: range2098552
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2
end: 1867
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2098988
display id: annotation2098988
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098988/range2098988/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098988/range2098988/1
type: Range
persistent identity: range2098988
display id: range2098988
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3644
end: 3649
orientation: inline

BBa_K404241
type: ComponentDefinition
persistent identity: BBa K404241
display id: BBa_K404241
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]VP123 (ViralBrick-453-BAP)
creator: Freiburg Bioware 2010
created: 2010-09-19T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: PLA2
sequence annotation: VP3
sequence annotation: BR1
sequence annotation: A-spacer
sequence annotation: A-Spacer
sequence annotation: BR4
sequence annotation: VP2
sequence annotation: BAP
sequence annotation: BR3
sequence annotation: VP1
sequence annotation: CMV
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: BR2
sequence: https://synbiohub.org/public/igem/BBa_K404241_sequence/1

PLA2
type: SequenceAnnotation
persistent identity: annotation2094196
display id: annotation2094196
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094196/range2094196/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094196/range2094196/1
type: Range
persistent identity: range2094196
display id: range2094196
version: 1
direction: direction/forward
top level: BBa_K404241
start: 799
end: 976
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094201
display id: annotation2094201
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094201/range2094201/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094201/range2094201/1
type: Range
persistent identity: range2094201
display id: range2094201
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1274
end: 2926
orientation: inline

BR1
type: SequenceAnnotation
persistent identity: annotation2094197
display id: annotation2094197
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094197/range2094197/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094197/range2094197/1
type: Range
persistent identity: range2094197
display id: range2094197
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1025
end: 1045
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2094191
display id: annotation2094191
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094191/range2094191/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094191/range2094191/1
type: Range
persistent identity: range2094191
display id: range2094191
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2075
end: 2077
orientation: inline

A-Spacer
type: SequenceAnnotation
persistent identity: annotation2094203
display id: annotation2094203
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094203/range2094203/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094203/range2094203/1
type: Range
persistent identity: range2094203
display id: range2094203
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2027
end: 2029
orientation: inline

BR4
type: SequenceAnnotation
persistent identity: annotation2094202
display id: annotation2094202
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094202/range2094202/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094202/range2094202/1
type: Range
persistent identity: range2094202
display id: range2094202
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1586
end: 1603
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094198
display id: annotation2094198
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094198/range2094198/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094198/range2094198/1
type: Range
persistent identity: range2094198
display id: range2094198
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1079
end: 2926
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2094204
display id: annotation2094204
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094204/range2094204/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094204/range2094204/1
type: Range
persistent identity: range2094204
display id: range2094204
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2030
end: 2074
orientation: inline

BR3
type: SequenceAnnotation
persistent identity: annotation2094200
display id: annotation2094200
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094200/range2094200/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094200/range2094200/1
type: Range
persistent identity: range2094200
display id: range2094200
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1163
end: 1183
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094195
display id: annotation2094195
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094195/range2094195/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094195/range2094195/1
type: Range
persistent identity: range2094195
display id: range2094195
version: 1
direction: direction/forward
top level: BBa_K404241
start: 671
end: 2926
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094193
display id: annotation2094193
version: 1
top level: BBa_K404241
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094193/range2094193/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094193/range2094193/1
type: Range
persistent identity: range2094193
display id: range2094193
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2
end: 655
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094194
display id: annotation2094194
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094194/range2094194/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094194/range2094194/1
type: Range
persistent identity: range2094194
display id: range2094194
version: 1
direction: direction/forward
top level: BBa_K404241
start: 671
end: 3049
orientation: inline

BR2
type: SequenceAnnotation
persistent identity: annotation2094199
display id: annotation2094199
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094199/range2094199/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094199/range2094199/1
type: Range
persistent identity: range2094199
display id: range2094199
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1085
end: 1105
orientation: inline

BBa_K404008
type: ComponentDefinition
persistent identity: BBa K404008
display id: BBa_K404008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP1
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404008_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2107872
display id: annotation2107872
version: 1
top level: BBa_K404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404008/annotation2107872/range2107872/1

https://synbiohub.org/public/igem/BBa_K404008/annotation2107872/range2107872/1
type: Range
persistent identity: range2107872
display id: range2107872
version: 1
direction: direction/forward
top level: BBa_K404008
start: 1
end: 2202
orientation: inline

BBa_K404003
type: ComponentDefinition
persistent identity: BBa K404003
display id: BBa_K404003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123(ViralBrick-587KO-empty)_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAP
sequence annotation: VP3
sequence annotation: A20-2
sequence annotation: p40
sequence annotation: basic region 3
sequence annotation: scar
sequence annotation: AAV-2-cap
sequence annotation: A20-4
sequence annotation: HSPG-KO
sequence annotation: VP1
sequence annotation: Rep68
sequence annotation: p5TATAless
sequence annotation: VP2
sequence annotation: Rep52
sequence annotation: Rep78
sequence annotation: basic region 1
sequence annotation: PLA2
sequence annotation: basic region 2
sequence annotation: B1
sequence annotation: A20-1
sequence annotation: p19
sequence annotation: basic region 4
sequence annotation: Rep40
sequence: https://synbiohub.org/public/igem/BBa_K404003_sequence/1

AAP
type: SequenceAnnotation
persistent identity: annotation2102747
display id: annotation2102747
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102747/range2102747/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102747/range2102747/1
type: Range
persistent identity: range2102747
display id: range2102747
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2409
end: 3023
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2102705
display id: annotation2102705
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102705/range2102705/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102705/range2102705/1
type: Range
persistent identity: range2102705
display id: range2102705
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2489
end: 4090
orientation: inline

A20-2
type: SequenceAnnotation
persistent identity: annotation2102708
display id: annotation2102708
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102708/range2102708/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102708/range2102708/1
type: Range
persistent identity: range2102708
display id: range2102708
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2990
end: 3016
orientation: inline

p40
type: SequenceAnnotation
persistent identity: annotation2102698
display id: annotation2102698
version: 1
top level: BBa_K404003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102698/range2102698/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102698/range2102698/1
type: Range
persistent identity: range2102698
display id: range2102698
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1390
end: 1507
orientation: inline

basic region 3
type: SequenceAnnotation
persistent identity: annotation2102704
display id: annotation2102704
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102704/range2102704/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102704/range2102704/1
type: Range
persistent identity: range2102704
display id: range2102704
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2378
end: 2398
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2102750
display id: annotation2102750
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102750/range2102750/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102750/range2102750/1
type: Range
persistent identity: range2102750
display id: range2102750
version: 1
direction: direction/forward
top level: BBa_K404003
start: 4214
end: 4221
orientation: inline

AAV-2-cap
type: SequenceAnnotation
persistent identity: annotation2102749
display id: annotation2102749
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102749/range2102749/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102749/range2102749/1
type: Range
persistent identity: range2102749
display id: range2102749
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3694
end: 4213
orientation: inline

A20-4
type: SequenceAnnotation
persistent identity: annotation2102709
display id: annotation2102709
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102709/range2102709/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102709/range2102709/1
type: Range
persistent identity: range2102709
display id: range2102709
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3578
end: 3607
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2102711
display id: annotation2102711
version: 1
top level: BBa_K404003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102711/range2102711/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102711/range2102711/1
type: Range
persistent identity: range2102711
display id: range2102711
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3644
end: 3646
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2102699
display id: annotation2102699
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102699/range2102699/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102699/range2102699/1
type: Range
persistent identity: range2102699
display id: range2102699
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1833
end: 4090
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2102694
display id: annotation2102694
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102694/range2102694/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102694/range2102694/1
type: Range
persistent identity: range2102694
display id: range2102694
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1
end: 1933
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2102712
display id: annotation2102712
version: 1
top level: BBa_K404003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102712/range2102712/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102712/range2102712/1
type: Range
persistent identity: range2102712
display id: range2102712
version: 1
direction: direction/forward
top level: BBa_K404003
start: 4222
end: 4385
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2102702
display id: annotation2102702
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102702/range2102702/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102702/range2102702/1
type: Range
persistent identity: range2102702
display id: range2102702
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2294
end: 4090
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2102696
display id: annotation2102696
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102696/range2102696/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102696/range2102696/1
type: Range
persistent identity: range2102696
display id: range2102696
version: 1
direction: direction/forward
top level: BBa_K404003
start: 673
end: 1867
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2102693
display id: annotation2102693
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102693/range2102693/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102693/range2102693/1
type: Range
persistent identity: range2102693
display id: range2102693
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1
end: 1867
orientation: inline

basic region 1
type: SequenceAnnotation
persistent identity: annotation2102701
display id: annotation2102701
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102701/range2102701/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102701/range2102701/1
type: Range
persistent identity: range2102701
display id: range2102701
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2240
end: 2260
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2102710
display id: annotation2102710
version: 1
top level: BBa_K404003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102710/range2102710/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102710/range2102710/1
type: Range
persistent identity: range2102710
display id: range2102710
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3635
end: 3637
orientation: inline

PLA2
type: SequenceAnnotation
persistent identity: annotation2102700
display id: annotation2102700
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102700/range2102700/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102700/range2102700/1
type: Range
persistent identity: range2102700
display id: range2102700
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2015
end: 2191
orientation: inline

basic region 2
type: SequenceAnnotation
persistent identity: annotation2102703
display id: annotation2102703
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102703/range2102703/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102703/range2102703/1
type: Range
persistent identity: range2102703
display id: range2102703
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2300
end: 2320
orientation: inline

B1
type: SequenceAnnotation
persistent identity: annotation2102713
display id: annotation2102713
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102713/range2102713/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102713/range2102713/1
type: Range
persistent identity: range2102713
display id: range2102713
version: 1
direction: direction/forward
top level: BBa_K404003
start: 4058
end: 4081
orientation: inline

A20-1
type: SequenceAnnotation
persistent identity: annotation2102706
display id: annotation2102706
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102706/range2102706/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102706/range2102706/1
type: Range
persistent identity: range2102706
display id: range2102706
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2693
end: 2722
orientation: inline

p19
type: SequenceAnnotation
persistent identity: annotation2102695
display id: annotation2102695
version: 1
top level: BBa_K404003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102695/range2102695/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102695/range2102695/1
type: Range
persistent identity: range2102695
display id: range2102695
version: 1
direction: direction/forward
top level: BBa_K404003
start: 410
end: 529
orientation: inline

basic region 4
type: SequenceAnnotation
persistent identity: annotation2102707
display id: annotation2102707
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102707/range2102707/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102707/range2102707/1
type: Range
persistent identity: range2102707
display id: range2102707
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2801
end: 2818
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2102697
display id: annotation2102697
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102697/range2102697/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102697/range2102697/1
type: Range
persistent identity: range2102697
display id: range2102697
version: 1
direction: direction/forward
top level: BBa_K404003
start: 673
end: 1933
orientation: inline

BBa_K404230
type: ComponentDefinition
persistent identity: BBa K404230
display id: BBa_K404230
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404158
type: ComponentDefinition
persistent identity: BBa K404158
display id: BBa_K404158
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_SEG-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: HSPG-KO
sequence annotation: CMV
sequence annotation: SEG-linker
sequence annotation: VP2
sequence annotation: ZEGFR_1907
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404158_sequence/1

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101795
display id: annotation2101795
version: 1
top level: BBa_K404158
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2101795/range2101795/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2101795/range2101795/1
type: Range
persistent identity: range2101795
display id: range2101795
version: 1
direction: direction/forward
top level: BBa_K404158
start: 2303
end: 2305
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101794
display id: annotation2101794
version: 1
top level: BBa_K404158
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2101794/range2101794/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2101794/range2101794/1
type: Range
persistent identity: range2101794
display id: range2101794
version: 1
direction: direction/forward
top level: BBa_K404158
start: 2312
end: 2314
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095000
display id: annotation2095000
version: 1
top level: BBa_K404158
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095000/range2095000/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095000/range2095000/1
type: Range
persistent identity: range2095000
display id: range2095000
version: 1
direction: direction/forward
top level: BBa_K404158
start: 2
end: 655
orientation: inline

SEG-linker
type: SequenceAnnotation
persistent identity: annotation2095002
display id: annotation2095002
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095002/range2095002/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095002/range2095002/1
type: Range
persistent identity: range2095002
display id: range2095002
version: 1
direction: direction/forward
top level: BBa_K404158
start: 851
end: 858
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095003
display id: annotation2095003
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095003/range2095003/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095003/range2095003/1
type: Range
persistent identity: range2095003
display id: range2095003
version: 1
direction: direction/forward
top level: BBa_K404158
start: 965
end: 2758
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2095001
display id: annotation2095001
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095001/range2095001/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095001/range2095001/1
type: Range
persistent identity: range2095001
display id: range2095001
version: 1
direction: direction/forward
top level: BBa_K404158
start: 671
end: 844
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095005
display id: annotation2095005
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095005/range2095005/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095005/range2095005/1
type: Range
persistent identity: range2095005
display id: range2095005
version: 1
direction: direction/forward
top level: BBa_K404158
start: 965
end: 2881
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095004
display id: annotation2095004
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095004/range2095004/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095004/range2095004/1
type: Range
persistent identity: range2095004
display id: range2095004
version: 1
direction: direction/forward
top level: BBa_K404158
start: 1157
end: 2758
orientation: inline

BBa_K404004
type: ComponentDefinition
persistent identity: BBa K404004
display id: BBa_K404004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP13(ViralBrick-587KO-empty)_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: basic region 3
sequence annotation: HSPG-KO
sequence annotation: p40
sequence annotation: basic region 2
sequence annotation: p19
sequence annotation: p5TATAless
sequence annotation: VP1
sequence annotation: Rep52
sequence annotation: Rep78
sequence annotation: HSPK-KO
sequence annotation: Rep68
sequence annotation: VP3
sequence annotation: G--> C VP2 KO
sequence annotation: basic region 1
sequence annotation: PLA2
sequence annotation: basic region 4
sequence annotation: Rep40
sequence annotation: scar
sequence: https://synbiohub.org/public/igem/BBa_K404004_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2099615
display id: annotation2099615
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099615/range2099615/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099615/range2099615/1
type: Range
persistent identity: range2099615
display id: range2099615
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1883
end: 4216
orientation: inline

basic region 3
type: SequenceAnnotation
persistent identity: annotation2099620
display id: annotation2099620
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099620/range2099620/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099620/range2099620/1
type: Range
persistent identity: range2099620
display id: range2099620
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2378
end: 2398
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2099626
display id: annotation2099626
version: 1
top level: BBa_K404004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099626/range2099626/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099626/range2099626/1
type: Range
persistent identity: range2099626
display id: range2099626
version: 1
direction: direction/forward
top level: BBa_K404004
start: 3635
end: 3637
orientation: inline

p40
type: SequenceAnnotation
persistent identity: annotation2099614
display id: annotation2099614
version: 1
top level: BBa_K404004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099614/range2099614/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099614/range2099614/1
type: Range
persistent identity: range2099614
display id: range2099614
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1390
end: 1507
orientation: inline

basic region 2
type: SequenceAnnotation
persistent identity: annotation2099619
display id: annotation2099619
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099619/range2099619/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099619/range2099619/1
type: Range
persistent identity: range2099619
display id: range2099619
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2300
end: 2320
orientation: inline

p19
type: SequenceAnnotation
persistent identity: annotation2099611
display id: annotation2099611
version: 1
top level: BBa_K404004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099611/range2099611/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099611/range2099611/1
type: Range
persistent identity: range2099611
display id: range2099611
version: 1
direction: direction/forward
top level: BBa_K404004
start: 410
end: 529
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2099623
display id: annotation2099623
version: 1
top level: BBa_K404004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099623/range2099623/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099623/range2099623/1
type: Range
persistent identity: range2099623
display id: range2099623
version: 1
direction: direction/forward
top level: BBa_K404004
start: 4328
end: 4401
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2099616
display id: annotation2099616
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099616/range2099616/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099616/range2099616/1
type: Range
persistent identity: range2099616
display id: range2099616
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1883
end: 4090
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2099613
display id: annotation2099613
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099613/range2099613/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099613/range2099613/1
type: Range
persistent identity: range2099613
display id: range2099613
version: 1
direction: direction/forward
top level: BBa_K404004
start: 673
end: 1867
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2099610
display id: annotation2099610
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099610/range2099610/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099610/range2099610/1
type: Range
persistent identity: range2099610
display id: range2099610
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1
end: 1867
orientation: inline

HSPK-KO
type: SequenceAnnotation
persistent identity: annotation2099625
display id: annotation2099625
version: 1
top level: BBa_K404004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099625/range2099625/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099625/range2099625/1
type: Range
persistent identity: range2099625
display id: range2099625
version: 1
direction: direction/forward
top level: BBa_K404004
start: 3644
end: 3646
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2099609
display id: annotation2099609
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099609/range2099609/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099609/range2099609/1
type: Range
persistent identity: range2099609
display id: range2099609
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1
end: 1933
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2099621
display id: annotation2099621
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099621/range2099621/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099621/range2099621/1
type: Range
persistent identity: range2099621
display id: range2099621
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2489
end: 4090
orientation: inline

G--> C VP2 KO
type: SequenceAnnotation
persistent identity: annotation2099624
display id: annotation2099624
version: 1
top level: BBa_K404004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099624/range2099624/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099624/range2099624/1
type: Range
persistent identity: range2099624
display id: range2099624
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2296
end: 2296
orientation: inline

basic region 1
type: SequenceAnnotation
persistent identity: annotation2099618
display id: annotation2099618
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099618/range2099618/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099618/range2099618/1
type: Range
persistent identity: range2099618
display id: range2099618
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2240
end: 2260
orientation: inline

PLA2
type: SequenceAnnotation
persistent identity: annotation2099617
display id: annotation2099617
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099617/range2099617/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099617/range2099617/1
type: Range
persistent identity: range2099617
display id: range2099617
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2015
end: 2191
orientation: inline

basic region 4
type: SequenceAnnotation
persistent identity: annotation2099622
display id: annotation2099622
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099622/range2099622/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099622/range2099622/1
type: Range
persistent identity: range2099622
display id: range2099622
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2801
end: 2818
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2099612
display id: annotation2099612
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099612/range2099612/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099612/range2099612/1
type: Range
persistent identity: range2099612
display id: range2099612
version: 1
direction: direction/forward
top level: BBa_K404004
start: 673
end: 1933
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101675
display id: annotation2101675
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2101675/range2101675/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2101675/range2101675/1
type: Range
persistent identity: range2101675
display id: range2101675
version: 1
direction: direction/forward
top level: BBa_K404004
start: 4217
end: 4327
orientation: inline

BBa_K404243
type: ComponentDefinition
persistent identity: BBa K404243
display id: BBa_K404243
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]VP123 (ViralBrick-453-RGD)
creator: Freiburg Bioware 2010
created: 2010-09-19T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: A-spacer
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: VP1
sequence annotation: VP2
sequence annotation: downstream 453
sequence annotation: RGD
sequence annotation: upstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404243_sequence/1

A-spacer
type: SequenceAnnotation
persistent identity: annotation2094250
display id: annotation2094250
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094250/range2094250/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094250/range2094250/1
type: Range
persistent identity: range2094250
display id: range2094250
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2027
end: 2029
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094248
display id: annotation2094248
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094248/range2094248/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094248/range2094248/1
type: Range
persistent identity: range2094248
display id: range2094248
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1720
end: 3031
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094244
display id: annotation2094244
version: 1
top level: BBa_K404243
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094244/range2094244/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094244/range2094244/1
type: Range
persistent identity: range2094244
display id: range2094244
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094247
display id: annotation2094247
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094247/range2094247/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094247/range2094247/1
type: Range
persistent identity: range2094247
display id: range2094247
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1274
end: 2908
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094245
display id: annotation2094245
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094245/range2094245/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094245/range2094245/1
type: Range
persistent identity: range2094245
display id: range2094245
version: 1
direction: direction/forward
top level: BBa_K404243
start: 671
end: 2908
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2094253
display id: annotation2094253
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094253/range2094253/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094253/range2094253/1
type: Range
persistent identity: range2094253
display id: range2094253
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2057
end: 2059
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094246
display id: annotation2094246
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094246/range2094246/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094246/range2094246/1
type: Range
persistent identity: range2094246
display id: range2094246
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1079
end: 2908
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2094252
display id: annotation2094252
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094252/range2094252/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094252/range2094252/1
type: Range
persistent identity: range2094252
display id: range2094252
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2060
end: 2073
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2094251
display id: annotation2094251
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094251/range2094251/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094251/range2094251/1
type: Range
persistent identity: range2094251
display id: range2094251
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2030
end: 2056
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2094249
display id: annotation2094249
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094249/range2094249/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094249/range2094249/1
type: Range
persistent identity: range2094249
display id: range2094249
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1989
end: 2026
orientation: inline

BBa_K404164
type: ComponentDefinition
persistent identity: BBa K404164
display id: BBa_K404164
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_VP1up_NLS_Z-EGFR-1907_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: VP1up
sequence annotation: ZEGFR_1907
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: HSPG-KO
sequence annotation: NLS
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404164_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2095191
display id: annotation2095191
version: 1
top level: BBa_K404164
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095191/range2095191/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095191/range2095191/1
type: Range
persistent identity: range2095191
display id: range2095191
version: 1
direction: direction/forward
top level: BBa_K404164
start: 2
end: 655
orientation: inline

VP1up
type: SequenceAnnotation
persistent identity: annotation2095192
display id: annotation2095192
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095192/range2095192/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095192/range2095192/1
type: Range
persistent identity: range2095192
display id: range2095192
version: 1
direction: direction/forward
top level: BBa_K404164
start: 673
end: 1081
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2095193
display id: annotation2095193
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095193/range2095193/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095193/range2095193/1
type: Range
persistent identity: range2095193
display id: range2095193
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1115
end: 1228
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095195
display id: annotation2095195
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095195/range2095195/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095195/range2095195/1
type: Range
persistent identity: range2095195
display id: range2095195
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1487
end: 3088
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095194
display id: annotation2095194
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095194/range2095194/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095194/range2095194/1
type: Range
persistent identity: range2095194
display id: range2095194
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1295
end: 3088
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101815
display id: annotation2101815
version: 1
top level: BBa_K404164
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2101815/range2101815/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2101815/range2101815/1
type: Range
persistent identity: range2101815
display id: range2101815
version: 1
direction: direction/forward
top level: BBa_K404164
start: 2633
end: 2635
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101814
display id: annotation2101814
version: 1
top level: BBa_K404164
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2101814/range2101814/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2101814/range2101814/1
type: Range
persistent identity: range2101814
display id: range2101814
version: 1
direction: direction/forward
top level: BBa_K404164
start: 2642
end: 2644
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2095197
display id: annotation2095197
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095197/range2095197/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095197/range2095197/1
type: Range
persistent identity: range2095197
display id: range2095197
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1088
end: 1108
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095199
display id: annotation2095199
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095199/range2095199/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095199/range2095199/1
type: Range
persistent identity: range2095199
display id: range2095199
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1295
end: 3211
orientation: inline

BBa_K404246
type: ComponentDefinition
persistent identity: BBa K404246
display id: BBa_K404246
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-BAP)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: downstream 587
sequence annotation: TG-spacer
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP3
sequence annotation: GLS-spacer
sequence annotation: BAP
sequence annotation: VP2
sequence annotation: CMV promoter
sequence annotation: upstream 587
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404246_sequence/1

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094277
display id: annotation2094277
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094277/range2094277/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094277/range2094277/1
type: Range
persistent identity: range2094277
display id: range2094277
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2489
end: 2504
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094275
display id: annotation2094275
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094275/range2094275/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094275/range2094275/1
type: Range
persistent identity: range2094275
display id: range2094275
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2429
end: 2434
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094273
display id: annotation2094273
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094273/range2094273/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094273/range2094273/1
type: Range
persistent identity: range2094273
display id: range2094273
version: 1
direction: direction/forward
top level: BBa_K404246
start: 671
end: 3058
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094271
display id: annotation2094271
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094271/range2094271/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094271/range2094271/1
type: Range
persistent identity: range2094271
display id: range2094271
version: 1
direction: direction/forward
top level: BBa_K404246
start: 1274
end: 2935
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094276
display id: annotation2094276
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094276/range2094276/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094276/range2094276/1
type: Range
persistent identity: range2094276
display id: range2094276
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2480
end: 2488
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2094274
display id: annotation2094274
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094274/range2094274/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094274/range2094274/1
type: Range
persistent identity: range2094274
display id: range2094274
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2435
end: 2479
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094267
display id: annotation2094267
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094267/range2094267/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094267/range2094267/1
type: Range
persistent identity: range2094267
display id: range2094267
version: 1
direction: direction/forward
top level: BBa_K404246
start: 1079
end: 2935
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094264
display id: annotation2094264
version: 1
top level: BBa_K404246
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094264/range2094264/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094264/range2094264/1
type: Range
persistent identity: range2094264
display id: range2094264
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2
end: 655
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094278
display id: annotation2094278
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094278/range2094278/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094278/range2094278/1
type: Range
persistent identity: range2094278
display id: range2094278
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2397
end: 2428
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094266
display id: annotation2094266
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094266/range2094266/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094266/range2094266/1
type: Range
persistent identity: range2094266
display id: range2094266
version: 1
direction: direction/forward
top level: BBa_K404246
start: 671
end: 2935
orientation: inline

BBa_K404165
type: ComponentDefinition
persistent identity: BBa K404165
display id: BBa_K404165
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_VP1up_NLS_mVenus_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP 2
sequence annotation: NLS
sequence annotation: VP 1
sequence annotation: mVenus
sequence annotation: AAP
sequence annotation: VP 3
sequence annotation: HSPG-KO
sequence annotation: VP 1 up
sequence annotation: AAV-2 cap (Stratagene)
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404165_sequence/1

VP 2
type: SequenceAnnotation
persistent identity: annotation2099098
display id: annotation2099098
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099098/range2099098/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099098/range2099098/1
type: Range
persistent identity: range2099098
display id: range2099098
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1835
end: 3628
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2099096
display id: annotation2099096
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099096/range2099096/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099096/range2099096/1
type: Range
persistent identity: range2099096
display id: range2099096
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1088
end: 1108
orientation: inline

VP 1
type: SequenceAnnotation
persistent identity: annotation2099100
display id: annotation2099100
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099100/range2099100/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099100/range2099100/1
type: Range
persistent identity: range2099100
display id: range2099100
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1835
end: 3628
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2099097
display id: annotation2099097
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099097/range2099097/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099097/range2099097/1
type: Range
persistent identity: range2099097
display id: range2099097
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1115
end: 1828
orientation: inline

AAP
type: SequenceAnnotation
persistent identity: annotation2099101
display id: annotation2099101
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099101/range2099101/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099101/range2099101/1
type: Range
persistent identity: range2099101
display id: range2099101
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1947
end: 2561
orientation: inline

VP 3
type: SequenceAnnotation
persistent identity: annotation2099093
display id: annotation2099093
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099093/range2099093/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099093/range2099093/1
type: Range
persistent identity: range2099093
display id: range2099093
version: 1
direction: direction/forward
top level: BBa_K404165
start: 2027
end: 3628
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101816
display id: annotation2101816
version: 1
top level: BBa_K404165
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2101816/range2101816/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2101816/range2101816/1
type: Range
persistent identity: range2101816
display id: range2101816
version: 1
direction: direction/forward
top level: BBa_K404165
start: 3182
end: 3184
orientation: inline

VP 1 up
type: SequenceAnnotation
persistent identity: annotation2099095
display id: annotation2099095
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099095/range2099095/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099095/range2099095/1
type: Range
persistent identity: range2099095
display id: range2099095
version: 1
direction: direction/forward
top level: BBa_K404165
start: 673
end: 1081
orientation: inline

AAV-2 cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2099099
display id: annotation2099099
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099099/range2099099/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099099/range2099099/1
type: Range
persistent identity: range2099099
display id: range2099099
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1835
end: 3751
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101817
display id: annotation2101817
version: 1
top level: BBa_K404165
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2101817/range2101817/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2101817/range2101817/1
type: Range
persistent identity: range2101817
display id: range2101817
version: 1
direction: direction/forward
top level: BBa_K404165
start: 3173
end: 3175
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2099094
display id: annotation2099094
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099094/range2099094/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099094/range2099094/1
type: Range
persistent identity: range2099094
display id: range2099094
version: 1
direction: direction/forward
top level: BBa_K404165
start: 2
end: 655
orientation: inline

BBa_K404252
type: ComponentDefinition
persistent identity: BBa K404252
display id: BBa_K404252
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: CMV promoter
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: downstream 598
sequence annotation: upstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404252_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094564
display id: annotation2094564
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094564/range2094564/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094564/range2094564/1
type: Range
persistent identity: range2094564
display id: range2094564
version: 1
direction: direction/forward
top level: BBa_K404252
start: 671
end: 2998
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094563
display id: annotation2094563
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094563/range2094563/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094563/range2094563/1
type: Range
persistent identity: range2094563
display id: range2094563
version: 1
direction: direction/forward
top level: BBa_K404252
start: 671
end: 2875
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094566
display id: annotation2094566
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094566/range2094566/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094566/range2094566/1
type: Range
persistent identity: range2094566
display id: range2094566
version: 1
direction: direction/forward
top level: BBa_K404252
start: 1274
end: 2875
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094562
display id: annotation2094562
version: 1
top level: BBa_K404252
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094562/range2094562/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094562/range2094562/1
type: Range
persistent identity: range2094562
display id: range2094562
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101878
display id: annotation2101878
version: 1
top level: BBa_K404252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2101878/range2101878/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2101878/range2101878/1
type: Range
persistent identity: range2101878
display id: range2101878
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2419
end: 2421
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094565
display id: annotation2094565
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094565/range2094565/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094565/range2094565/1
type: Range
persistent identity: range2094565
display id: range2094565
version: 1
direction: direction/forward
top level: BBa_K404252
start: 1079
end: 2875
orientation: inline

downstream 598
type: SequenceAnnotation
persistent identity: annotation2094568
display id: annotation2094568
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094568/range2094568/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094568/range2094568/1
type: Range
persistent identity: range2094568
display id: range2094568
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2429
end: 2444
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094567
display id: annotation2094567
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094567/range2094567/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094567/range2094567/1
type: Range
persistent identity: range2094567
display id: range2094567
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2397
end: 2428
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101877
display id: annotation2101877
version: 1
top level: BBa_K404252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2101877/range2101877/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2101877/range2101877/1
type: Range
persistent identity: range2101877
display id: range2101877
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2429
end: 2431
orientation: inline

BBa_K404160
type: ComponentDefinition
persistent identity: BBa K404160
display id: BBa_K404160
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_His-Tag_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: His-tag
sequence annotation: middle.linker
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404160_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097925
display id: annotation2097925
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097925/range2097925/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097925/range2097925/1
type: Range
persistent identity: range2097925
display id: range2097925
version: 1
direction: direction/forward
top level: BBa_K404160
start: 725
end: 2641
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101799
display id: annotation2101799
version: 1
top level: BBa_K404160
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2101799/range2101799/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2101799/range2101799/1
type: Range
persistent identity: range2101799
display id: range2101799
version: 1
direction: direction/forward
top level: BBa_K404160
start: 2072
end: 2074
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097923
display id: annotation2097923
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097923/range2097923/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097923/range2097923/1
type: Range
persistent identity: range2097923
display id: range2097923
version: 1
direction: direction/forward
top level: BBa_K404160
start: 725
end: 2518
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097924
display id: annotation2097924
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097924/range2097924/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097924/range2097924/1
type: Range
persistent identity: range2097924
display id: range2097924
version: 1
direction: direction/forward
top level: BBa_K404160
start: 917
end: 2518
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2097921
display id: annotation2097921
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097921/range2097921/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097921/range2097921/1
type: Range
persistent identity: range2097921
display id: range2097921
version: 1
direction: direction/forward
top level: BBa_K404160
start: 671
end: 688
orientation: inline

middle.linker
type: SequenceAnnotation
persistent identity: annotation2097922
display id: annotation2097922
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097922/range2097922/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097922/range2097922/1
type: Range
persistent identity: range2097922
display id: range2097922
version: 1
direction: direction/forward
top level: BBa_K404160
start: 695
end: 718
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101800
display id: annotation2101800
version: 1
top level: BBa_K404160
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2101800/range2101800/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2101800/range2101800/1
type: Range
persistent identity: range2101800
display id: range2101800
version: 1
direction: direction/forward
top level: BBa_K404160
start: 2063
end: 2065
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097920
display id: annotation2097920
version: 1
top level: BBa_K404160
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097920/range2097920/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097920/range2097920/1
type: Range
persistent identity: range2097920
display id: range2097920
version: 1
direction: direction/forward
top level: BBa_K404160
start: 2
end: 655
orientation: inline

BBa_K404151
type: ComponentDefinition
persistent identity: BBa K404151
display id: BBa_K404151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: HSPG-KO
sequence: https://synbiohub.org/public/igem/BBa_K404151_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2095298
display id: annotation2095298
version: 1
top level: BBa_K404151
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2095298/range2095298/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2095298/range2095298/1
type: Range
persistent identity: range2095298
display id: range2095298
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1
end: 1794
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095299
display id: annotation2095299
version: 1
top level: BBa_K404151
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2095299/range2095299/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2095299/range2095299/1
type: Range
persistent identity: range2095299
display id: range2095299
version: 1
direction: direction/forward
top level: BBa_K404151
start: 193
end: 1794
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095300
display id: annotation2095300
version: 1
top level: BBa_K404151
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2095300/range2095300/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2095300/range2095300/1
type: Range
persistent identity: range2095300
display id: range2095300
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1
end: 1917
orientation: inline

HSPG-KO
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persistent identity: annotation2101716
display id: annotation2101716
version: 1
top level: BBa_K404151
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2101716/range2101716/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2101716/range2101716/1
type: Range
persistent identity: range2101716
display id: range2101716
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1339
end: 1341
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101715
display id: annotation2101715
version: 1
top level: BBa_K404151
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2101715/range2101715/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2101715/range2101715/1
type: Range
persistent identity: range2101715
display id: range2101715
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1348
end: 1350
orientation: inline

BBa_K404156
type: ComponentDefinition
persistent identity: BBa K404156
display id: BBa_K404156
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: HSPG-KO
sequence annotation: middle-linker
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: ZEGFR_1907
sequence: https://synbiohub.org/public/igem/BBa_K404156_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2094980
display id: annotation2094980
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094980/range2094980/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094980/range2094980/1
type: Range
persistent identity: range2094980
display id: range2094980
version: 1
direction: direction/forward
top level: BBa_K404156
start: 881
end: 2674
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094991
display id: annotation2094991
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094991/range2094991/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094991/range2094991/1
type: Range
persistent identity: range2094991
display id: range2094991
version: 1
direction: direction/forward
top level: BBa_K404156
start: 881
end: 2797
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101782
display id: annotation2101782
version: 1
top level: BBa_K404156
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2101782/range2101782/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2101782/range2101782/1
type: Range
persistent identity: range2101782
display id: range2101782
version: 1
direction: direction/forward
top level: BBa_K404156
start: 2219
end: 2221
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2094992
display id: annotation2094992
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094992/range2094992/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094992/range2094992/1
type: Range
persistent identity: range2094992
display id: range2094992
version: 1
direction: direction/forward
top level: BBa_K404156
start: 851
end: 874
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094979
display id: annotation2094979
version: 1
top level: BBa_K404156
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094979/range2094979/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094979/range2094979/1
type: Range
persistent identity: range2094979
display id: range2094979
version: 1
direction: direction/forward
top level: BBa_K404156
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094981
display id: annotation2094981
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094981/range2094981/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094981/range2094981/1
type: Range
persistent identity: range2094981
display id: range2094981
version: 1
direction: direction/forward
top level: BBa_K404156
start: 1073
end: 2674
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101755
display id: annotation2101755
version: 1
top level: BBa_K404156
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2101755/range2101755/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2101755/range2101755/1
type: Range
persistent identity: range2101755
display id: range2101755
version: 1
direction: direction/forward
top level: BBa_K404156
start: 2228
end: 2230
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2094993
display id: annotation2094993
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094993/range2094993/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094993/range2094993/1
type: Range
persistent identity: range2094993
display id: range2094993
version: 1
direction: direction/forward
top level: BBa_K404156
start: 671
end: 844
orientation: inline

BBa_K404256
type: ComponentDefinition
persistent identity: BBa K404256
display id: BBa_K404256
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Z34C-Spacer)
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: AAV--1-cap (Stratagene)
sequence annotation: GLS-spacer
sequence annotation: Z34C
sequence annotation: HSPG-KO
sequence annotation: TG-spacer
sequence annotation: VP2
sequence annotation: CMV
sequence annotation: upstream 587
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404256_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2094727
display id: annotation2094727
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094727/range2094727/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094727/range2094727/1
type: Range
persistent identity: range2094727
display id: range2094727
version: 1
direction: direction/forward
top level: BBa_K404256
start: 671
end: 2992
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094729
display id: annotation2094729
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094729/range2094729/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094729/range2094729/1
type: Range
persistent identity: range2094729
display id: range2094729
version: 1
direction: direction/forward
top level: BBa_K404256
start: 1274
end: 2992
orientation: inline

AAV--1-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094730
display id: annotation2094730
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094730/range2094730/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094730/range2094730/1
type: Range
persistent identity: range2094730
display id: range2094730
version: 1
direction: direction/forward
top level: BBa_K404256
start: 671
end: 3115
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094734
display id: annotation2094734
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094734/range2094734/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094734/range2094734/1
type: Range
persistent identity: range2094734
display id: range2094734
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2537
end: 2545
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2094731
display id: annotation2094731
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094731/range2094731/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094731/range2094731/1
type: Range
persistent identity: range2094731
display id: range2094731
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2435
end: 2536
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101929
display id: annotation2101929
version: 1
top level: BBa_K404256
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2101929/range2101929/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2101929/range2101929/1
type: Range
persistent identity: range2101929
display id: range2101929
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2433
end: 2435
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101894
display id: annotation2101894
version: 1
top level: BBa_K404256
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2101894/range2101894/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2101894/range2101894/1
type: Range
persistent identity: range2101894
display id: range2101894
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2546
end: 2548
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094733
display id: annotation2094733
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094733/range2094733/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094733/range2094733/1
type: Range
persistent identity: range2094733
display id: range2094733
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2429
end: 2434
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094728
display id: annotation2094728
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094728/range2094728/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094728/range2094728/1
type: Range
persistent identity: range2094728
display id: range2094728
version: 1
direction: direction/forward
top level: BBa_K404256
start: 1079
end: 2992
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094726
display id: annotation2094726
version: 1
top level: BBa_K404256
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094726/range2094726/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094726/range2094726/1
type: Range
persistent identity: range2094726
display id: range2094726
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2
end: 655
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094736
display id: annotation2094736
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094736/range2094736/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094736/range2094736/1
type: Range
persistent identity: range2094736
display id: range2094736
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2397
end: 2428
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094735
display id: annotation2094735
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094735/range2094735/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094735/range2094735/1
type: Range
persistent identity: range2094735
display id: range2094735
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2546
end: 2561
orientation: inline

BBa_K404244
type: ComponentDefinition
persistent identity: BBa K404244
display id: BBa_K404244
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]VP123 (ViralBrick-453-Z34C)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV promoter
sequence annotation: AAV-2-cap (Strategene)
sequence annotation: VP2
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: Z34C
sequence: https://synbiohub.org/public/igem/BBa_K404244_sequence/1

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094254
display id: annotation2094254
version: 1
top level: BBa_K404244
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094254/range2094254/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094254/range2094254/1
type: Range
persistent identity: range2094254
display id: range2094254
version: 1
direction: direction/forward
top level: BBa_K404244
start: 2
end: 655
orientation: inline

AAV-2-cap (Strategene)
type: SequenceAnnotation
persistent identity: annotation2094258
display id: annotation2094258
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094258/range2094258/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094258/range2094258/1
type: Range
persistent identity: range2094258
display id: range2094258
version: 1
direction: direction/forward
top level: BBa_K404244
start: 671
end: 3100
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094256
display id: annotation2094256
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094256/range2094256/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094256/range2094256/1
type: Range
persistent identity: range2094256
display id: range2094256
version: 1
direction: direction/forward
top level: BBa_K404244
start: 1079
end: 2977
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094255
display id: annotation2094255
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094255/range2094255/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094255/range2094255/1
type: Range
persistent identity: range2094255
display id: range2094255
version: 1
direction: direction/forward
top level: BBa_K404244
start: 671
end: 2977
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094257
display id: annotation2094257
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094257/range2094257/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094257/range2094257/1
type: Range
persistent identity: range2094257
display id: range2094257
version: 1
direction: direction/forward
top level: BBa_K404244
start: 1274
end: 2977
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2094259
display id: annotation2094259
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094259/range2094259/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094259/range2094259/1
type: Range
persistent identity: range2094259
display id: range2094259
version: 1
direction: direction/forward
top level: BBa_K404244
start: 2027
end: 2128
orientation: inline

BBa_K404163
type: ComponentDefinition
persistent identity: BBa K404163
display id: BBa_K404163
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: His-tag
sequence annotation: basic region 4
sequence annotation: upstream 587
sequence annotation: A20-1 binding site
sequence annotation: GLS-spacer
sequence annotation: scar
sequence annotation: A20-2 binding site
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: downstream 587
sequence annotation: HSPG-KO
sequence annotation: middle-linker
sequence annotation: TG-spacer
sequence annotation: ZEGFR_1907
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence annotation: B1 binding site
sequence annotation: basic region 3
sequence annotation: basic region 2
sequence annotation: A20-4 binding site
sequence: https://synbiohub.org/public/igem/BBa_K404163_sequence/1

His-tag
type: SequenceAnnotation
persistent identity: annotation2101706
display id: annotation2101706
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101706/range2101706/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101706/range2101706/1
type: Range
persistent identity: range2101706
display id: range2101706
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2234
end: 2251
orientation: inline

basic region 4
type: SequenceAnnotation
persistent identity: annotation2101700
display id: annotation2101700
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101700/range2101700/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101700/range2101700/1
type: Range
persistent identity: range2101700
display id: range2101700
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1385
end: 1402
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2101703
display id: annotation2101703
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101703/range2101703/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101703/range2101703/1
type: Range
persistent identity: range2101703
display id: range2101703
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2196
end: 2227
orientation: inline

A20-1 binding site
type: SequenceAnnotation
persistent identity: annotation2101699
display id: annotation2101699
version: 1
top level: BBa_K404163
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101699/range2101699/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101699/range2101699/1
type: Range
persistent identity: range2101699
display id: range2101699
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1277
end: 1306
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2101707
display id: annotation2101707
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101707/range2101707/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101707/range2101707/1
type: Range
persistent identity: range2101707
display id: range2101707
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2252
end: 2260
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101691
display id: annotation2101691
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101691/range2101691/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101691/range2101691/1
type: Range
persistent identity: range2101691
display id: range2101691
version: 1
direction: direction/forward
top level: BBa_K404163
start: 845
end: 850
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101693
display id: annotation2101693
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101693/range2101693/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101693/range2101693/1
type: Range
persistent identity: range2101693
display id: range2101693
version: 1
direction: direction/forward
top level: BBa_K404163
start: 875
end: 880
orientation: inline

A20-2 binding site
type: SequenceAnnotation
persistent identity: annotation2101701
display id: annotation2101701
version: 1
top level: BBa_K404163
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101701/range2101701/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101701/range2101701/1
type: Range
persistent identity: range2101701
display id: range2101701
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1574
end: 1600
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2101688
display id: annotation2101688
version: 1
top level: BBa_K404163
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101688/range2101688/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101688/range2101688/1
type: Range
persistent identity: range2101688
display id: range2101688
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2101698
display id: annotation2101698
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101698/range2101698/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101698/range2101698/1
type: Range
persistent identity: range2101698
display id: range2101698
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1073
end: 2707
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101689
display id: annotation2101689
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101689/range2101689/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101689/range2101689/1
type: Range
persistent identity: range2101689
display id: range2101689
version: 1
direction: direction/forward
top level: BBa_K404163
start: 656
end: 670
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2101708
display id: annotation2101708
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101708/range2101708/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101708/range2101708/1
type: Range
persistent identity: range2101708
display id: range2101708
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2261
end: 2276
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101709
display id: annotation2101709
version: 1
top level: BBa_K404163
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101709/range2101709/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101709/range2101709/1
type: Range
persistent identity: range2101709
display id: range2101709
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2288
end: 2290
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2101692
display id: annotation2101692
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101692/range2101692/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101692/range2101692/1
type: Range
persistent identity: range2101692
display id: range2101692
version: 1
direction: direction/forward
top level: BBa_K404163
start: 851
end: 874
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2101705
display id: annotation2101705
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101705/range2101705/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101705/range2101705/1
type: Range
persistent identity: range2101705
display id: range2101705
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2228
end: 2233
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2101690
display id: annotation2101690
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101690/range2101690/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101690/range2101690/1
type: Range
persistent identity: range2101690
display id: range2101690
version: 1
direction: direction/forward
top level: BBa_K404163
start: 671
end: 844
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101704
display id: annotation2101704
version: 1
top level: BBa_K404163
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101704/range2101704/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101704/range2101704/1
type: Range
persistent identity: range2101704
display id: range2101704
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2219
end: 2221
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2101695
display id: annotation2101695
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101695/range2101695/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101695/range2101695/1
type: Range
persistent identity: range2101695
display id: range2101695
version: 1
direction: direction/forward
top level: BBa_K404163
start: 881
end: 2830
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2101694
display id: annotation2101694
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101694/range2101694/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101694/range2101694/1
type: Range
persistent identity: range2101694
display id: range2101694
version: 1
direction: direction/forward
top level: BBa_K404163
start: 881
end: 2707
orientation: inline

B1 binding site
type: SequenceAnnotation
persistent identity: annotation2101710
display id: annotation2101710
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101710/range2101710/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101710/range2101710/1
type: Range
persistent identity: range2101710
display id: range2101710
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2675
end: 2698
orientation: inline

basic region 3
type: SequenceAnnotation
persistent identity: annotation2101697
display id: annotation2101697
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101697/range2101697/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101697/range2101697/1
type: Range
persistent identity: range2101697
display id: range2101697
version: 1
direction: direction/forward
top level: BBa_K404163
start: 962
end: 982
orientation: inline

basic region 2
type: SequenceAnnotation
persistent identity: annotation2101696
display id: annotation2101696
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101696/range2101696/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101696/range2101696/1
type: Range
persistent identity: range2101696
display id: range2101696
version: 1
direction: direction/forward
top level: BBa_K404163
start: 884
end: 904
orientation: inline

A20-4 binding site
type: SequenceAnnotation
persistent identity: annotation2101702
display id: annotation2101702
version: 1
top level: BBa_K404163
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101702/range2101702/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101702/range2101702/1
type: Range
persistent identity: range2101702
display id: range2101702
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2162
end: 2191
orientation: inline

BBa_K404152
type: ComponentDefinition
persistent identity: BBa K404152
display id: BBa_K404152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP1up
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1up
sequence: https://synbiohub.org/public/igem/BBa_K404152_sequence/1

VP1up
type: SequenceAnnotation
persistent identity: annotation2093343
display id: annotation2093343
version: 1
top level: BBa_K404152
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404152/annotation2093343/range2093343/1

https://synbiohub.org/public/igem/BBa_K404152/annotation2093343/range2093343/1
type: Range
persistent identity: range2093343
display id: range2093343
version: 1
direction: direction/forward
top level: BBa_K404152
start: 1
end: 411
orientation: inline

BBa_K404009
type: ComponentDefinition
persistent identity: BBa K404009
display id: BBa_K404009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP1
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404009_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2097901
display id: annotation2097901
version: 1
top level: BBa_K404009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404009/annotation2097901/range2097901/1

https://synbiohub.org/public/igem/BBa_K404009/annotation2097901/range2097901/1
type: Range
persistent identity: range2097901
display id: range2097901
version: 1
direction: direction/forward
top level: BBa_K404009
start: 2
end: 655
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2097902
display id: annotation2097902
version: 1
top level: BBa_K404009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404009/annotation2097902/range2097902/1

https://synbiohub.org/public/igem/BBa_K404009/annotation2097902/range2097902/1
type: Range
persistent identity: range2097902
display id: range2097902
version: 1
direction: direction/forward
top level: BBa_K404009
start: 670
end: 2910
orientation: inline

BBa_K404236
type: ComponentDefinition
persistent identity: BBa K404236
display id: BBa_K404236
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]Rep-VP123_p5TATAless (ViralBrick 453 RGD-587 HSPG-KO)
creator: Freiburg Bioware 2010
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: A-spacer
sequence annotation: p5TATAless
sequence annotation: HSPG-KO
sequence annotation: Rep78
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: Rep52
sequence annotation: RGD
sequence annotation: Rep68
sequence annotation: Rep40
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404236_sequence/1

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098412
display id: annotation2098412
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098412/range2098412/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098412/range2098412/1
type: Range
persistent identity: range2098412
display id: range2098412
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3242
end: 3244
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098429
display id: annotation2098429
version: 1
top level: BBa_K404236
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098429/range2098429/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098429/range2098429/1
type: Range
persistent identity: range2098429
display id: range2098429
version: 1
direction: direction/forward
top level: BBa_K404236
start: 4255
end: 4418
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101874
display id: annotation2101874
version: 1
top level: BBa_K404236
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2101874/range2101874/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2101874/range2101874/1
type: Range
persistent identity: range2101874
display id: range2101874
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3155
end: 3157
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098403
display id: annotation2098403
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098403/range2098403/1

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type: Range
persistent identity: range2098403
display id: range2098403
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2
end: 1867
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098409
display id: annotation2098409
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098409/range2098409/1

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type: Range
persistent identity: range2098409
display id: range2098409
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2294
end: 4123
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101873
display id: annotation2101873
version: 1
top level: BBa_K404236
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2101873/range2101873/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2101873/range2101873/1
type: Range
persistent identity: range2101873
display id: range2101873
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3308
end: 3310
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098410
display id: annotation2098410
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098410/range2098410/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098410/range2098410/1
type: Range
persistent identity: range2098410
display id: range2098410
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2489
end: 4123
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098406
display id: annotation2098406
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098406/range2098406/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098406/range2098406/1
type: Range
persistent identity: range2098406
display id: range2098406
version: 1
direction: direction/forward
top level: BBa_K404236
start: 673
end: 1867
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098413
display id: annotation2098413
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098413/range2098413/1

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type: Range
persistent identity: range2098413
display id: range2098413
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3272
end: 3274
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2098411
display id: annotation2098411
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098411/range2098411/1

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type: Range
persistent identity: range2098411
display id: range2098411
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3245
end: 3271
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098405
display id: annotation2098405
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098405/range2098405/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098405/range2098405/1
type: Range
persistent identity: range2098405
display id: range2098405
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2
end: 1933
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098407
display id: annotation2098407
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098407/range2098407/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098407/range2098407/1
type: Range
persistent identity: range2098407
display id: range2098407
version: 1
direction: direction/forward
top level: BBa_K404236
start: 673
end: 1933
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098408
display id: annotation2098408
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098408/range2098408/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098408/range2098408/1
type: Range
persistent identity: range2098408
display id: range2098408
version: 1
direction: direction/forward
top level: BBa_K404236
start: 1883
end: 4123
orientation: inline

BBa_K404154
type: ComponentDefinition
persistent identity: BBa K404154
display id: BBa_K404154
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: HSPG-KO
sequence annotation: CMV
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: ZEGFR_1907
sequence: https://synbiohub.org/public/igem/BBa_K404154_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097913
display id: annotation2097913
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097913/range2097913/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097913/range2097913/1
type: Range
persistent identity: range2097913
display id: range2097913
version: 1
direction: direction/forward
top level: BBa_K404154
start: 851
end: 2767
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101718
display id: annotation2101718
version: 1
top level: BBa_K404154
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2101718/range2101718/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2101718/range2101718/1
type: Range
persistent identity: range2101718
display id: range2101718
version: 1
direction: direction/forward
top level: BBa_K404154
start: 2189
end: 2191
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101717
display id: annotation2101717
version: 1
top level: BBa_K404154
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2101717/range2101717/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2101717/range2101717/1
type: Range
persistent identity: range2101717
display id: range2101717
version: 1
direction: direction/forward
top level: BBa_K404154
start: 2198
end: 2200
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097908
display id: annotation2097908
version: 1
top level: BBa_K404154
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097908/range2097908/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097908/range2097908/1
type: Range
persistent identity: range2097908
display id: range2097908
version: 1
direction: direction/forward
top level: BBa_K404154
start: 2
end: 655
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097910
display id: annotation2097910
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097910/range2097910/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097910/range2097910/1
type: Range
persistent identity: range2097910
display id: range2097910
version: 1
direction: direction/forward
top level: BBa_K404154
start: 851
end: 2644
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097912
display id: annotation2097912
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097912/range2097912/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097912/range2097912/1
type: Range
persistent identity: range2097912
display id: range2097912
version: 1
direction: direction/forward
top level: BBa_K404154
start: 1043
end: 2644
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2097909
display id: annotation2097909
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097909/range2097909/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097909/range2097909/1
type: Range
persistent identity: range2097909
display id: range2097909
version: 1
direction: direction/forward
top level: BBa_K404154
start: 671
end: 844
orientation: inline

BBa_K404168
type: ComponentDefinition
persistent identity: BBa K404168
display id: BBa_K404168
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_VP1up_NLS_mVenus_VP2/3(ViralBrick-587-KO-His)
creator: Freiburg Bioware 2010
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: HSPG-KO
sequence annotation: GLS-spacer
sequence annotation: VP1up
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: mVenus
sequence annotation: upstream 587
sequence annotation: His-tag
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: TG-spacer
sequence annotation: NLS
sequence annotation: VP2
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404168_sequence/1

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101845
display id: annotation2101845
version: 1
top level: BBa_K404168
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2101845/range2101845/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2101845/range2101845/1
type: Range
persistent identity: range2101845
display id: range2101845
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3163
end: 3165
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2095290
display id: annotation2095290
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095290/range2095290/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095290/range2095290/1
type: Range
persistent identity: range2095290
display id: range2095290
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3206
end: 3214
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101844
display id: annotation2101844
version: 1
top level: BBa_K404168
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2101844/range2101844/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2101844/range2101844/1
type: Range
persistent identity: range2101844
display id: range2101844
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3215
end: 3217
orientation: inline

VP1up
type: SequenceAnnotation
persistent identity: annotation2095281
display id: annotation2095281
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095281/range2095281/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095281/range2095281/1
type: Range
persistent identity: range2095281
display id: range2095281
version: 1
direction: direction/forward
top level: BBa_K404168
start: 673
end: 1081
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095279
display id: annotation2095279
version: 1
top level: BBa_K404168
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095279/range2095279/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095279/range2095279/1
type: Range
persistent identity: range2095279
display id: range2095279
version: 1
direction: direction/forward
top level: BBa_K404168
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095286
display id: annotation2095286
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095286/range2095286/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095286/range2095286/1
type: Range
persistent identity: range2095286
display id: range2095286
version: 1
direction: direction/forward
top level: BBa_K404168
start: 2027
end: 3661
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2095282
display id: annotation2095282
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095282/range2095282/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095282/range2095282/1
type: Range
persistent identity: range2095282
display id: range2095282
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1115
end: 1828
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2095293
display id: annotation2095293
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095293/range2095293/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095293/range2095293/1
type: Range
persistent identity: range2095293
display id: range2095293
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3150
end: 3181
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2095287
display id: annotation2095287
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095287/range2095287/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095287/range2095287/1
type: Range
persistent identity: range2095287
display id: range2095287
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3188
end: 3205
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095294
display id: annotation2095294
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095294/range2095294/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095294/range2095294/1
type: Range
persistent identity: range2095294
display id: range2095294
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1835
end: 3784
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2095288
display id: annotation2095288
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095288/range2095288/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095288/range2095288/1
type: Range
persistent identity: range2095288
display id: range2095288
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3182
end: 3187
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2095284
display id: annotation2095284
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095284/range2095284/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095284/range2095284/1
type: Range
persistent identity: range2095284
display id: range2095284
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1088
end: 1108
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095285
display id: annotation2095285
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095285/range2095285/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095285/range2095285/1
type: Range
persistent identity: range2095285
display id: range2095285
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1835
end: 3661
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2095292
display id: annotation2095292
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095292/range2095292/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095292/range2095292/1
type: Range
persistent identity: range2095292
display id: range2095292
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3215
end: 3230
orientation: inline

BBa_K404010
type: ComponentDefinition
persistent identity: BBa K404010
display id: BBa_K404010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP2
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404010_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2093158
display id: annotation2093158
version: 1
top level: BBa_K404010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404010/annotation2093158/range2093158/1

https://synbiohub.org/public/igem/BBa_K404010/annotation2093158/range2093158/1
type: Range
persistent identity: range2093158
display id: range2093158
version: 1
direction: direction/forward
top level: BBa_K404010
start: 1
end: 1830
orientation: inline

BBa_K404250
type: ComponentDefinition
persistent identity: BBa K404250
display id: BBa_K404250
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-Beta-lactamase)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP3
sequence annotation: upstream 587
sequence annotation: VP2
sequence annotation: VP1
sequence annotation: CMV promoter
sequence annotation: Beta-lactamase
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404250_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094506
display id: annotation2094506
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094506/range2094506/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094506/range2094506/1
type: Range
persistent identity: range2094506
display id: range2094506
version: 1
direction: direction/forward
top level: BBa_K404250
start: 671
end: 3787
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094505
display id: annotation2094505
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094505/range2094505/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094505/range2094505/1
type: Range
persistent identity: range2094505
display id: range2094505
version: 1
direction: direction/forward
top level: BBa_K404250
start: 1274
end: 3664
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094508
display id: annotation2094508
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094508/range2094508/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094508/range2094508/1
type: Range
persistent identity: range2094508
display id: range2094508
version: 1
direction: direction/forward
top level: BBa_K404250
start: 2397
end: 2428
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094504
display id: annotation2094504
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094504/range2094504/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094504/range2094504/1
type: Range
persistent identity: range2094504
display id: range2094504
version: 1
direction: direction/forward
top level: BBa_K404250
start: 1079
end: 3664
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094449
display id: annotation2094449
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094449/range2094449/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094449/range2094449/1
type: Range
persistent identity: range2094449
display id: range2094449
version: 1
direction: direction/forward
top level: BBa_K404250
start: 671
end: 3664
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094448
display id: annotation2094448
version: 1
top level: BBa_K404250
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094448/range2094448/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094448/range2094448/1
type: Range
persistent identity: range2094448
display id: range2094448
version: 1
direction: direction/forward
top level: BBa_K404250
start: 2
end: 655
orientation: inline

Beta-lactamase
type: SequenceAnnotation
persistent identity: annotation2094507
display id: annotation2094507
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094507/range2094507/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094507/range2094507/1
type: Range
persistent identity: range2094507
display id: range2094507
version: 1
direction: direction/forward
top level: BBa_K404250
start: 2429
end: 3217
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094509
display id: annotation2094509
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094509/range2094509/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094509/range2094509/1
type: Range
persistent identity: range2094509
display id: range2094509
version: 1
direction: direction/forward
top level: BBa_K404250
start: 3218
end: 3233
orientation: inline

BBa_K404161
type: ComponentDefinition
persistent identity: BBa K404161
display id: BBa_K404161
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_DARPin-E01_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: DARPin-E01
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: middle-linker
sequence annotation: HSPG-KO
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404161_sequence/1

DARPin-E01
type: SequenceAnnotation
persistent identity: annotation2095007
display id: annotation2095007
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095007/range2095007/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095007/range2095007/1
type: Range
persistent identity: range2095007
display id: range2095007
version: 1
direction: direction/forward
top level: BBa_K404161
start: 666
end: 1130
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095006
display id: annotation2095006
version: 1
top level: BBa_K404161
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095006/range2095006/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095006/range2095006/1
type: Range
persistent identity: range2095006
display id: range2095006
version: 1
direction: direction/forward
top level: BBa_K404161
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095010
display id: annotation2095010
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095010/range2095010/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095010/range2095010/1
type: Range
persistent identity: range2095010
display id: range2095010
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1358
end: 2959
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2095008
display id: annotation2095008
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095008/range2095008/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095008/range2095008/1
type: Range
persistent identity: range2095008
display id: range2095008
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1136
end: 1159
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101801
display id: annotation2101801
version: 1
top level: BBa_K404161
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2101801/range2101801/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2101801/range2101801/1
type: Range
persistent identity: range2101801
display id: range2101801
version: 1
direction: direction/forward
top level: BBa_K404161
start: 2513
end: 2515
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095011
display id: annotation2095011
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095011/range2095011/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095011/range2095011/1
type: Range
persistent identity: range2095011
display id: range2095011
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1166
end: 3082
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101802
display id: annotation2101802
version: 1
top level: BBa_K404161
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2101802/range2101802/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2101802/range2101802/1
type: Range
persistent identity: range2101802
display id: range2101802
version: 1
direction: direction/forward
top level: BBa_K404161
start: 2504
end: 2506
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095009
display id: annotation2095009
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095009/range2095009/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095009/range2095009/1
type: Range
persistent identity: range2095009
display id: range2095009
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1166
end: 2959
orientation: inline

BBa_K404226
type: ComponentDefinition
persistent identity: BBa K404226
display id: BBa_K404226
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-587-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404013
type: ComponentDefinition
persistent identity: BBa K404013
display id: BBa_K404013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP3
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404013_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2097907
display id: annotation2097907
version: 1
top level: BBa_K404013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404013/annotation2097907/range2097907/1

https://synbiohub.org/public/igem/BBa_K404013/annotation2097907/range2097907/1
type: Range
persistent identity: range2097907
display id: range2097907
version: 1
direction: direction/forward
top level: BBa_K404013
start: 670
end: 2305
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097906
display id: annotation2097906
version: 1
top level: BBa_K404013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404013/annotation2097906/range2097906/1

https://synbiohub.org/public/igem/BBa_K404013/annotation2097906/range2097906/1
type: Range
persistent identity: range2097906
display id: range2097906
version: 1
direction: direction/forward
top level: BBa_K404013
start: 2
end: 655
orientation: inline

BBa_K404228
type: ComponentDefinition
persistent identity: BBa K404228
display id: BBa_K404228
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATA-KO (ViralBrick-587-BLA)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: true
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project

BBa_K404223
type: ComponentDefinition
persistent identity: BBa K404223
display id: BBa_K404223
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_p5-TATAless (ViralBrick-453-RGD)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: VP1
sequence annotation: RGD
sequence annotation: Rep40
sequence annotation: Rep68
sequence annotation: VP2
sequence annotation: Rep78
sequence annotation: A-spacer
sequence annotation: p5TATAless
sequence annotation: Rep52
sequence: https://synbiohub.org/public/igem/BBa_K404223_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2098396
display id: annotation2098396
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098396/range2098396/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098396/range2098396/1
type: Range
persistent identity: range2098396
display id: range2098396
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2489
end: 4123
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098369
display id: annotation2098369
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098369/range2098369/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098369/range2098369/1
type: Range
persistent identity: range2098369
display id: range2098369
version: 1
direction: direction/forward
top level: BBa_K404223
start: 1883
end: 4123
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2098398
display id: annotation2098398
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098398/range2098398/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098398/range2098398/1
type: Range
persistent identity: range2098398
display id: range2098398
version: 1
direction: direction/forward
top level: BBa_K404223
start: 3245
end: 3271
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098341
display id: annotation2098341
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098341/range2098341/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098341/range2098341/1
type: Range
persistent identity: range2098341
display id: range2098341
version: 1
direction: direction/forward
top level: BBa_K404223
start: 673
end: 1933
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098340
display id: annotation2098340
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098340/range2098340/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098340/range2098340/1
type: Range
persistent identity: range2098340
display id: range2098340
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2
end: 1933
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098370
display id: annotation2098370
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098370/range2098370/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098370/range2098370/1
type: Range
persistent identity: range2098370
display id: range2098370
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2294
end: 4123
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098338
display id: annotation2098338
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098338/range2098338/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098338/range2098338/1
type: Range
persistent identity: range2098338
display id: range2098338
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2
end: 1867
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098399
display id: annotation2098399
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098399/range2098399/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098399/range2098399/1
type: Range
persistent identity: range2098399
display id: range2098399
version: 1
direction: direction/forward
top level: BBa_K404223
start: 3242
end: 3244
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098397
display id: annotation2098397
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098397/range2098397/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098397/range2098397/1
type: Range
persistent identity: range2098397
display id: range2098397
version: 1
direction: direction/forward
top level: BBa_K404223
start: 4255
end: 4418
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098400
display id: annotation2098400
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098400/range2098400/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098400/range2098400/1
type: Range
persistent identity: range2098400
display id: range2098400
version: 1
direction: direction/forward
top level: BBa_K404223
start: 3272
end: 3274
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098342
display id: annotation2098342
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098342/range2098342/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098342/range2098342/1
type: Range
persistent identity: range2098342
display id: range2098342
version: 1
direction: direction/forward
top level: BBa_K404223
start: 673
end: 1867
orientation: inline

BBa_K404248
type: ComponentDefinition
persistent identity: BBa K404248
display id: BBa_K404248
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-RGD)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: CMV promoter
sequence annotation: upstream 587
sequence annotation: RGD
sequence annotation: downstream 587
sequence annotation: VP3
sequence annotation: TG-spacer
sequence annotation: VP2
sequence annotation: GLS-spacer
sequence: https://synbiohub.org/public/igem/BBa_K404248_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2094295
display id: annotation2094295
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094295/range2094295/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094295/range2094295/1
type: Range
persistent identity: range2094295
display id: range2094295
version: 1
direction: direction/forward
top level: BBa_K404248
start: 671
end: 2917
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094316
display id: annotation2094316
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094316/range2094316/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094316/range2094316/1
type: Range
persistent identity: range2094316
display id: range2094316
version: 1
direction: direction/forward
top level: BBa_K404248
start: 671
end: 3040
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094294
display id: annotation2094294
version: 1
top level: BBa_K404248
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094294/range2094294/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094294/range2094294/1
type: Range
persistent identity: range2094294
display id: range2094294
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2
end: 655
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094390
display id: annotation2094390
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094390/range2094390/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094390/range2094390/1
type: Range
persistent identity: range2094390
display id: range2094390
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2397
end: 2428
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2094371
display id: annotation2094371
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094371/range2094371/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094371/range2094371/1
type: Range
persistent identity: range2094371
display id: range2094371
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2435
end: 2461
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094391
display id: annotation2094391
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094391/range2094391/1

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type: Range
persistent identity: range2094391
display id: range2094391
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2471
end: 2486
orientation: inline

VP3
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persistent identity: annotation2094315
display id: annotation2094315
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094315/range2094315/1

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type: Range
persistent identity: range2094315
display id: range2094315
version: 1
direction: direction/forward
top level: BBa_K404248
start: 1274
end: 2917
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094410
display id: annotation2094410
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094410/range2094410/1

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type: Range
persistent identity: range2094410
display id: range2094410
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2429
end: 2434
orientation: inline

VP2
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persistent identity: annotation2094314
display id: annotation2094314
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094314/range2094314/1

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type: Range
persistent identity: range2094314
display id: range2094314
version: 1
direction: direction/forward
top level: BBa_K404248
start: 1079
end: 2917
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094429
display id: annotation2094429
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094429/range2094429/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094429/range2094429/1
type: Range
persistent identity: range2094429
display id: range2094429
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2462
end: 2470
orientation: inline

BBa_K404210
type: ComponentDefinition
persistent identity: BBa K404210
display id: BBa_K404210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587KO-Empty
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: R=>A
sequence annotation: upstream 587
sequence annotation: downstream 587
sequence annotation: upstream 453
sequence annotation: downstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404210_sequence/1

R=>A
type: SequenceAnnotation
persistent identity: annotation2079130
display id: annotation2079130
version: 1
top level: BBa_K404210
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079130/range2079130/1

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type: Range
persistent identity: range2079130
display id: range2079130
version: 1
direction: direction/forward
top level: BBa_K404210
start: 43
end: 45
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079126
display id: annotation2079126
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079126/range2079126/1

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type: Range
persistent identity: range2079126
display id: range2079126
version: 1
direction: direction/forward
top level: BBa_K404210
start: 10
end: 42
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079127
display id: annotation2079127
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079127/range2079127/1

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type: Range
persistent identity: range2079127
display id: range2079127
version: 1
direction: direction/forward
top level: BBa_K404210
start: 43
end: 63
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079129
display id: annotation2079129
version: 1
top level: BBa_K404210
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079129/range2079129/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079129/range2079129/1
type: Range
persistent identity: range2079129
display id: range2079129
version: 1
direction: direction/forward
top level: BBa_K404210
start: 34
end: 36
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079125
display id: annotation2079125
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079125/range2079125/1

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type: Range
persistent identity: range2079125
display id: range2079125
version: 1
direction: direction/forward
top level: BBa_K404210
start: 1
end: 9
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079128
display id: annotation2079128
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079128/range2079128/1

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type: Range
persistent identity: range2079128
display id: range2079128
version: 1
direction: direction/forward
top level: BBa_K404210
start: 64
end: 72
orientation: inline

BBa_K404166
type: ComponentDefinition
persistent identity: BBa K404166
display id: BBa_K404166
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_VP1up_NLS_His-Tag_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: HSPG-KO
sequence annotation: VP1up
sequence annotation: VP2
sequence annotation: CMV
sequence annotation: NLS
sequence annotation: His-tag
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404166_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2094650
display id: annotation2094650
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094650/range2094650/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094650/range2094650/1
type: Range
persistent identity: range2094650
display id: range2094650
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1332
end: 2933
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101830
display id: annotation2101830
version: 1
top level: BBa_K404166
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2101830/range2101830/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2101830/range2101830/1
type: Range
persistent identity: range2101830
display id: range2101830
version: 1
direction: direction/forward
top level: BBa_K404166
start: 2478
end: 2480
orientation: inline

VP1up
type: SequenceAnnotation
persistent identity: annotation2094644
display id: annotation2094644
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094644/range2094644/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094644/range2094644/1
type: Range
persistent identity: range2094644
display id: range2094644
version: 1
direction: direction/forward
top level: BBa_K404166
start: 674
end: 1082
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094648
display id: annotation2094648
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094648/range2094648/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094648/range2094648/1
type: Range
persistent identity: range2094648
display id: range2094648
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1140
end: 2933
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094643
display id: annotation2094643
version: 1
top level: BBa_K404166
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094643/range2094643/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094643/range2094643/1
type: Range
persistent identity: range2094643
display id: range2094643
version: 1
direction: direction/forward
top level: BBa_K404166
start: 2
end: 655
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2094645
display id: annotation2094645
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094645/range2094645/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094645/range2094645/1
type: Range
persistent identity: range2094645
display id: range2094645
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1089
end: 1109
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2094646
display id: annotation2094646
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094646/range2094646/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094646/range2094646/1
type: Range
persistent identity: range2094646
display id: range2094646
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1116
end: 1133
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094647
display id: annotation2094647
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094647/range2094647/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094647/range2094647/1
type: Range
persistent identity: range2094647
display id: range2094647
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1140
end: 3056
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094649
display id: annotation2094649
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094649/range2094649/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094649/range2094649/1
type: Range
persistent identity: range2094649
display id: range2094649
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1140
end: 2933
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101818
display id: annotation2101818
version: 1
top level: BBa_K404166
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2101818/range2101818/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2101818/range2101818/1
type: Range
persistent identity: range2101818
display id: range2101818
version: 1
direction: direction/forward
top level: BBa_K404166
start: 2487
end: 2489
orientation: inline

BBa_K404150
type: ComponentDefinition
persistent identity: BBa K404150
display id: BBa_K404150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP23
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404150_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2093338
display id: annotation2093338
version: 1
top level: BBa_K404150
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404150/annotation2093338/range2093338/1

https://synbiohub.org/public/igem/BBa_K404150/annotation2093338/range2093338/1
type: Range
persistent identity: range2093338
display id: range2093338
version: 1
direction: direction/forward
top level: BBa_K404150
start: 193
end: 1794
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2093337
display id: annotation2093337
version: 1
top level: BBa_K404150
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404150/annotation2093337/range2093337/1

https://synbiohub.org/public/igem/BBa_K404150/annotation2093337/range2093337/1
type: Range
persistent identity: range2093337
display id: range2093337
version: 1
direction: direction/forward
top level: BBa_K404150
start: 1
end: 1794
orientation: inline

BBa_K404251
type: ComponentDefinition
persistent identity: BBa K404251
display id: BBa_K404251
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-BAP)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: downstream 587
sequence annotation: GLS-spacer
sequence annotation: VP2
sequence annotation: VP1
sequence annotation: CMV promoter
sequence annotation: upstream 587
sequence annotation: HSPG-KO
sequence annotation: BAP
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: TG-spacer
sequence: https://synbiohub.org/public/igem/BBa_K404251_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2094525
display id: annotation2094525
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094525/range2094525/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094525/range2094525/1
type: Range
persistent identity: range2094525
display id: range2094525
version: 1
direction: direction/forward
top level: BBa_K404251
start: 1274
end: 2935
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094530
display id: annotation2094530
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094530/range2094530/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094530/range2094530/1
type: Range
persistent identity: range2094530
display id: range2094530
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2489
end: 2504
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094529
display id: annotation2094529
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094529/range2094529/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094529/range2094529/1
type: Range
persistent identity: range2094529
display id: range2094529
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2480
end: 2488
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094524
display id: annotation2094524
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094524/range2094524/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094524/range2094524/1
type: Range
persistent identity: range2094524
display id: range2094524
version: 1
direction: direction/forward
top level: BBa_K404251
start: 1079
end: 2935
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094522
display id: annotation2094522
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094522/range2094522/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094522/range2094522/1
type: Range
persistent identity: range2094522
display id: range2094522
version: 1
direction: direction/forward
top level: BBa_K404251
start: 671
end: 2935
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094521
display id: annotation2094521
version: 1
top level: BBa_K404251
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094521/range2094521/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094521/range2094521/1
type: Range
persistent identity: range2094521
display id: range2094521
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2
end: 655
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094526
display id: annotation2094526
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094526/range2094526/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094526/range2094526/1
type: Range
persistent identity: range2094526
display id: range2094526
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2397
end: 2428
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101876
display id: annotation2101876
version: 1
top level: BBa_K404251
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2101876/range2101876/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2101876/range2101876/1
type: Range
persistent identity: range2101876
display id: range2101876
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2419
end: 2421
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101875
display id: annotation2101875
version: 1
top level: BBa_K404251
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2101875/range2101875/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2101875/range2101875/1
type: Range
persistent identity: range2101875
display id: range2101875
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2481
end: 2483
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2094528
display id: annotation2094528
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094528/range2094528/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094528/range2094528/1
type: Range
persistent identity: range2094528
display id: range2094528
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2435
end: 2479
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094523
display id: annotation2094523
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094523/range2094523/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094523/range2094523/1
type: Range
persistent identity: range2094523
display id: range2094523
version: 1
direction: direction/forward
top level: BBa_K404251
start: 671
end: 3058
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094527
display id: annotation2094527
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094527/range2094527/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094527/range2094527/1
type: Range
persistent identity: range2094527
display id: range2094527
version: 1
direction: direction/forward
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