https://synbiohub.org/public/igem_feature_libraries/igem_plasmid_vectors/1
type: Collection
persistent identity: igem plasmid vectors
display id: igem_plasmid_vectors
version: 1
owned by: nroehner
member: BBa_J23019
member: BBa_K1480018
member: pSB1A2
member: https://synbiohub.org/public/igem/BBa_K864020/1
member: https://synbiohub.org/public/igem/BBa_J176127/1
member: BBa_K731710
member: BBa_K260002
member: BBa_K635002
member: BBa_K590011
member: https://synbiohub.org/public/igem/BBa_K2029015/1
member: BBa_K415049
member: https://synbiohub.org/public/igem/BBa_K864015/1
member: pSB4C50
member: https://synbiohub.org/public/igem/BBa_K788012/1
member: BBa_K1945011
member: BBa_K1438032
member: pSB3K1
member: BBa_K1641999
member: pSac-Cm_BB
member: https://synbiohub.org/public/igem/BBa_M36098/1
member: BBa_K1321301
member: https://synbiohub.org/public/igem/BBa_K300000/1
member: pSB1A8
member: BBa_K176007
member: https://synbiohub.org/public/igem/pSB4C5/1
member: https://synbiohub.org/public/igem/BBa_K125000/1
member: https://synbiohub.org/public/igem/BBa_K812300/1
member: BBa_M36097
member: BBa_J153000
member: BBa_K249004
member: BBa_K572200
member: V0120
member: BBa_K300001
member: BBa_K1971011
member: BBa_K260001
member: pSB8C15FRT
member: pSB4K50
member: BBa_K268000
member: BBa_K533012
member: BBa_J99000
member: pTG262-BB
member: BBa_J61003
member: BBa_K260003
member: pSBLB4C15
member: BBa_K1084201
member: BBa_K319033
member: BBa_K289008
member: pSB4A50
member: pSB1A3 lac
member: B. S. Vect
member: BBa_K289012
member: BBa_K238026
member: BBa_K245005
member: BBa_K560013
member: https://synbiohub.org/public/igem/BBa_K864009/1
member: BBa_I750017
member: lcp
member: https://synbiohub.org/public/igem/BBa_K2170300/1
member: BBa_K209498
member: https://synbiohub.org/public/igem/BBa_M36096/1
member: BBa_J99003
member: pSB2K4
member: https://synbiohub.org/public/igem/BBa_I732950/1
member: BBa_K245007
member: BBa_K1680014
member: https://synbiohub.org/public/igem/BBa_J100119/1
member: pSB1K16
member: BBa_K1795400
member: BBa_K1438031
member: BBa_K788005
member: BBa_K209473
member: pSB4A1
member: pSB8C15
member: Adh1P Acce
member: pSB8K15FRT

BBa_K788005
type: ComponentDefinition
persistent identity: BBa K788005
display id: BBa_K788005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psb1c3 and pzp
creator: Jia-Bao Chen
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1043_
m user id: 0
owner id: 11770
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

BBa_K106693
type: ComponentDefinition
persistent identity: BBa K106693
display id: BBa_K106693
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)
creator: Andrew Horwitz
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is a special variant acceptor vector for AarI cloning. The "A" site used here, termed "A!" is an alternate end used when no amino-terminal fusions are tolerated. Please see the UCSF 2008 iGEM wiki for more information on AarI cloning. This was designed specifically for use with Sir3 and amino-terminal domains of Sir3.
mutable notes: Note that this vector will NOT accept standard AarI "A" parts.
mutable provenance: Modified pRS315 plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ampicillin resistance marker
sequence annotation: Adh1 terminator
sequence annotation: ampicillin resistance promoter
sequence annotation: pBR322 origin
sequence annotation: lacZalpha reporter
sequence annotation: Start codon
sequence annotation: AarI A!D MCS
sequence annotation: T3 promoter
sequence annotation: Adh1 promoter
sequence annotation: Adh1P! promoter
sequence annotation: LEU2 marker
sequence annotation: lac promoter
sequence annotation: A overhang
sequence annotation: T7 promoter
sequence annotation: f1 origin
sequence annotation: D overhang
sequence annotation: Stop codons in all 3 frames
sequence annotation: CEN ARS
sequence: https://synbiohub.org/public/igem/BBa_K106693_sequence/1

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1994690
display id: annotation1994690
version: 1
top level: BBa_K106693
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994690/range1994690/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994690/range1994690/1
type: Range
persistent identity: range1994690
display id: range1994690
version: 1
direction: direction/forward
top level: BBa_K106693
start: 4053
end: 4911
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1994693
display id: annotation1994693
version: 1
top level: BBa_K106693
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994693/range1994693/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994693/range1994693/1
type: Range
persistent identity: range1994693
display id: range1994693
version: 1
direction: direction/forward
top level: BBa_K106693
start: 18
end: 211
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999719
display id: annotation1999719
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999719/range1999719/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999719/range1999719/1
type: Range
persistent identity: range1999719
display id: range1999719
version: 1
direction: direction/forward
top level: BBa_K106693
start: 3983
end: 4011
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999720
display id: annotation1999720
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999720/range1999720/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999720/range1999720/1
type: Range
persistent identity: range1999720
display id: range1999720
version: 1
direction: direction/forward
top level: BBa_K106693
start: 5068
end: 5687
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999717
display id: annotation1999717
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999717/range1999717/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999717/range1999717/1
type: Range
persistent identity: range1999717
display id: range1999717
version: 1
direction: direction/forward
top level: BBa_K106693
start: 246
end: 405
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999726
display id: annotation1999726
version: 1
top level: BBa_K106693
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999726/range1999726/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999726/range1999726/1
type: Range
persistent identity: range1999726
display id: range1999726
version: 1
direction: direction/forward
top level: BBa_K106693
start: 7638
end: 7640
orientation: inline

AarI A!D MCS
type: SequenceAnnotation
persistent identity: annotation1994692
display id: annotation1994692
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994692/range1994692/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994692/range1994692/1
type: Range
persistent identity: range1994692
display id: range1994692
version: 1
direction: direction/forward
top level: BBa_K106693
start: 4305
end: 4381
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999722
display id: annotation1999722
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999722/range1999722/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999722/range1999722/1
type: Range
persistent identity: range1999722
display id: range1999722
version: 1
direction: direction/forward
top level: BBa_K106693
start: 6095
end: 6114
orientation: inline

Adh1 promoter
type: SequenceAnnotation
persistent identity: annotation1999723
display id: annotation1999723
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999723/range1999723/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999723/range1999723/1
type: Range
persistent identity: range1999723
display id: range1999723
version: 1
direction: direction/forward
top level: BBa_K106693
start: 7229
end: 7625
orientation: inline

Adh1P! promoter
type: SequenceAnnotation
persistent identity: annotation1994691
display id: annotation1994691
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994691/range1994691/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994691/range1994691/1
type: Range
persistent identity: range1994691
display id: range1994691
version: 1
direction: direction/forward
top level: BBa_K106693
start: 6132
end: 7642
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1994688
display id: annotation1994688
version: 1
top level: BBa_K106693
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994688/range1994688/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994688/range1994688/1
type: Range
persistent identity: range1994688
display id: range1994688
version: 1
direction: direction/forward
top level: BBa_K106693
start: 1570
end: 2676
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999721
display id: annotation1999721
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999721/range1999721/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999721/range1999721/1
type: Range
persistent identity: range1999721
display id: range1999721
version: 1
direction: direction/forward
top level: BBa_K106693
start: 5996
end: 6025
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999725
display id: annotation1999725
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999725/range1999725/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999725/range1999725/1
type: Range
persistent identity: range1999725
display id: range1999725
version: 1
direction: direction/forward
top level: BBa_K106693
start: 7647
end: 7650
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999716
display id: annotation1999716
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999716/range1999716/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999716/range1999716/1
type: Range
persistent identity: range1999716
display id: range1999716
version: 1
direction: direction/forward
top level: BBa_K106693
start: 224
end: 242
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999718
display id: annotation1999718
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999718/range1999718/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999718/range1999718/1
type: Range
persistent identity: range1999718
display id: range1999718
version: 1
direction: direction/forward
top level: BBa_K106693
start: 427
end: 733
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999724
display id: annotation1999724
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999724/range1999724/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999724/range1999724/1
type: Range
persistent identity: range1999724
display id: range1999724
version: 1
direction: direction/forward
top level: BBa_K106693
start: 1
end: 4
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999727
display id: annotation1999727
version: 1
top level: BBa_K106693
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999727/range1999727/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999727/range1999727/1
type: Range
persistent identity: range1999727
display id: range1999727
version: 1
direction: direction/forward
top level: BBa_K106693
start: 11
end: 21
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1994689
display id: annotation1994689
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994689/range1994689/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994689/range1994689/1
type: Range
persistent identity: range1994689
display id: range1994689
version: 1
direction: direction/reverse
top level: BBa_K106693
start: 3403
end: 3921
orientation: inline

BBa_K300001
type: ComponentDefinition
persistent identity: BBa K300001
display id: BBa_K300001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioBrick integrative base vector for S. cerevisiae
creator: Susanna Zucca, Lorenzo Pasotti, Federica Sampietro, Paolo Magni
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G0012
component: BBa_B0045
component: BBa_K300988
component: BBa_K300989
component: BBa_B0048
component: BBa_M31992
component: BBa_K300987
component: BBa_K300990
component: BBa_G00000
component: BBa_K300993
component: BBa_K300986
component: BBa_K300992
sequence annotation: BBa_K300993
sequence annotation: BBa_K300987
sequence annotation: BBa_K300988
sequence annotation: BBa_M31992
sequence annotation: BBa_B0045
sequence annotation: BBa_B0048
sequence annotation: BBa_K300986
sequence annotation: BBa_K300989
sequence annotation: BBa_G0012
sequence annotation: BBa_K300990
sequence annotation: BBa_K300992
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_K300001_sequence/1

BBa_G0012
type: Component
persistent identity: component2081762
display id: component2081762
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_G0012/1

BBa_B0045
type: Component
persistent identity: component2081777
display id: component2081777
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_K300988
type: Component
persistent identity: component2081787
display id: component2081787
version: 1
top level: BBa_K300001
access: public
definition: pSB1A2

BBa_K300989
type: Component
persistent identity: component2081772
display id: component2081772
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_K300989/1

BBa_B0048
type: Component
persistent identity: component2081791
display id: component2081791
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0048/1

BBa_M31992
type: Component
persistent identity: component2081763
display id: component2081763
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_M31992/1

BBa_K300987
type: Component
persistent identity: component2081775
display id: component2081775
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_K300987/1

BBa_B0048
type: Component
persistent identity: component2081794
display id: component2081794
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0048/1

BBa_K300990
type: Component
persistent identity: component2081778
display id: component2081778
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_K300990/1

BBa_B0045
type: Component
persistent identity: component2081774
display id: component2081774
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_G00000
type: Component
persistent identity: component2081799
display id: component2081799
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_K300993
type: Component
persistent identity: component2081788
display id: component2081788
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_K300993/1

BBa_K300990
type: Component
persistent identity: component2081789
display id: component2081789
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_K300990/1

BBa_K300986
type: Component
persistent identity: component2081792
display id: component2081792
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_K300986/1

BBa_K300992
type: Component
persistent identity: component2081779
display id: component2081779
version: 1
top level: BBa_K300001
access: public
definition: https://synbiohub.org/public/igem/BBa_K300992/1

BBa_K300993
type: SequenceAnnotation
persistent identity: annotation2081788
display id: annotation2081788
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081788/range2081788/1
component: BBa_K300993

https://synbiohub.org/public/igem/BBa_K300001/annotation2081788/range2081788/1
type: Range
persistent identity: range2081788
display id: range2081788
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 3656
end: 3661
orientation: inline

BBa_K300987
type: SequenceAnnotation
persistent identity: annotation2081775
display id: annotation2081775
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081775/range2081775/1
component: BBa_K300987

https://synbiohub.org/public/igem/BBa_K300001/annotation2081775/range2081775/1
type: Range
persistent identity: range2081775
display id: range2081775
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 1555
end: 1599
orientation: inline

BBa_K300988
type: SequenceAnnotation
persistent identity: annotation2081787
display id: annotation2081787
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081787/range2081787/1
component: BBa_K300988

https://synbiohub.org/public/igem/BBa_K300001/annotation2081787/range2081787/1
type: Range
persistent identity: range2081787
display id: range2081787
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 1620
end: 3655
orientation: inline

BBa_M31992
type: SequenceAnnotation
persistent identity: annotation2081763
display id: annotation2081763
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081763/range2081763/1
component: BBa_M31992

https://synbiohub.org/public/igem/BBa_K300001/annotation2081763/range2081763/1
type: Range
persistent identity: range2081763
display id: range2081763
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 2
end: 7
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2081777
display id: annotation2081777
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081777/range2081777/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_K300001/annotation2081777/range2081777/1
type: Range
persistent identity: range2081777
display id: range2081777
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 1600
end: 1605
orientation: inline

BBa_B0048
type: SequenceAnnotation
persistent identity: annotation2081791
display id: annotation2081791
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081791/range2081791/1
component: BBa_B0048

https://synbiohub.org/public/igem/BBa_K300001/annotation2081791/range2081791/1
type: Range
persistent identity: range2081791
display id: range2081791
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 3670
end: 3675
orientation: inline

BBa_K300986
type: SequenceAnnotation
persistent identity: annotation2081792
display id: annotation2081792
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081792/range2081792/1
component: BBa_K300986

https://synbiohub.org/public/igem/BBa_K300001/annotation2081792/range2081792/1
type: Range
persistent identity: range2081792
display id: range2081792
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 3676
end: 3720
orientation: inline

BBa_K300989
type: SequenceAnnotation
persistent identity: annotation2081772
display id: annotation2081772
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081772/range2081772/1
component: BBa_K300989

https://synbiohub.org/public/igem/BBa_K300001/annotation2081772/range2081772/1
type: Range
persistent identity: range2081772
display id: range2081772
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 8
end: 1548
orientation: inline

BBa_G0012
type: SequenceAnnotation
persistent identity: annotation2081762
display id: annotation2081762
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081762/range2081762/1
component: BBa_G0012

https://synbiohub.org/public/igem/BBa_K300001/annotation2081762/range2081762/1
type: Range
persistent identity: range2081762
display id: range2081762
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 1
end: 1
orientation: inline

BBa_K300990
type: SequenceAnnotation
persistent identity: annotation2081778
display id: annotation2081778
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081778/range2081778/1
component: BBa_K300990

https://synbiohub.org/public/igem/BBa_K300001/annotation2081778/range2081778/1
type: Range
persistent identity: range2081778
display id: range2081778
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 1606
end: 1613
orientation: inline

BBa_K300992
type: SequenceAnnotation
persistent identity: annotation2081779
display id: annotation2081779
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081779/range2081779/1
component: BBa_K300992

https://synbiohub.org/public/igem/BBa_K300001/annotation2081779/range2081779/1
type: Range
persistent identity: range2081779
display id: range2081779
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 1614
end: 1619
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2081774
display id: annotation2081774
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081774/range2081774/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_K300001/annotation2081774/range2081774/1
type: Range
persistent identity: range2081774
display id: range2081774
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 1549
end: 1554
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2081799
display id: annotation2081799
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081799/range2081799/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K300001/annotation2081799/range2081799/1
type: Range
persistent identity: range2081799
display id: range2081799
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 3727
end: 3748
orientation: inline

BBa_K300990
type: SequenceAnnotation
persistent identity: annotation2081789
display id: annotation2081789
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081789/range2081789/1
component: BBa_K300990

https://synbiohub.org/public/igem/BBa_K300001/annotation2081789/range2081789/1
type: Range
persistent identity: range2081789
display id: range2081789
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 3662
end: 3669
orientation: inline

BBa_B0048
type: SequenceAnnotation
persistent identity: annotation2081794
display id: annotation2081794
version: 1
top level: BBa_K300001
location: https://synbiohub.org/public/igem/BBa_K300001/annotation2081794/range2081794/1
component: BBa_B0048

https://synbiohub.org/public/igem/BBa_K300001/annotation2081794/range2081794/1
type: Range
persistent identity: range2081794
display id: range2081794
version: 1
direction: direction/reverse
top level: BBa_K300001
start: 3721
end: 3726
orientation: inline

BBa_K1437001
type: ComponentDefinition
persistent identity: BBa K1437001
display id: BBa_K1437001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PRS306 shuttle plasmid in yeast.
creator: Yifan Wu
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:25Z
discontinued: false
dominant: false
group u list: _1815_
m user id: 0
owner id: 11599
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PRS306 is a shuttle plasmid which can express great both in E.coli and yeast.It has Amp resistance and it is URA auxotroph.
mutable notes: No.
mutable provenance: It is a popular shuttle plasmid in yeast.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1437001_sequence/1

pSB1K16
type: ComponentDefinition
persistent identity: pSB1K16
display id: pSB1K16
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gateway entry vector for gene cassette (L1L2)
creator: Joy Jiao, Laura Deming, Adrian Slusarczyk
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _525_
m user id: 0
owner id: 5564
owning group id: 525
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part made by MIT 2010 iGEM Team
mutable notes: To be added
mutable provenance: To be added
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: attL1 Recombination Site
sequence annotation: attL2 Recombination Site
sequence: https://synbiohub.org/public/igem/pSB1K16_sequence/1

attL1 Recombination Site
type: SequenceAnnotation
persistent identity: annotation2104593
display id: annotation2104593
version: 1
top level: pSB1K16
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K16/annotation2104593/range2104593/1

https://synbiohub.org/public/igem/pSB1K16/annotation2104593/range2104593/1
type: Range
persistent identity: range2104593
display id: range2104593
version: 1
direction: direction/forward
top level: pSB1K16
start: 2446
end: 2544
orientation: inline

attL2 Recombination Site
type: SequenceAnnotation
persistent identity: annotation2104594
display id: annotation2104594
version: 1
top level: pSB1K16
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K16/annotation2104594/range2104594/1

https://synbiohub.org/public/igem/pSB1K16/annotation2104594/range2104594/1
type: Range
persistent identity: range2104594
display id: range2104594
version: 1
direction: direction/forward
top level: pSB1K16
start: 1
end: 100
orientation: inline

BBa_J107056
type: ComponentDefinition
persistent identity: BBa J107056
display id: BBa_J107056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4C5 with RBS-RFP-TT between SpeI-PstI
creator: Lorenzo Pasotti
created: 2011-08-11T11:00:00Z
modified: 2015-08-31T04:08:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _587_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: When the SpeI-PstI DNA fragment from BBa_J61002 , containing the mRFP1 gene with a strong RBS and a double terminator, is transferred into pSB4C5 (digested SpeI-PstI) the resulting plasmid is BBa_J107055 . See pSB4C5 for detailed feature information.
mutable notes: This is not a standard BioBrick plasmid, as the suffix is not SpeI-NotI-PstI.
mutable provenance: pSB3K3 (backbone) and BBa_J61002 (SpeI-PstI insert).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J107056_sequence/1

V0120
type: ComponentDefinition
persistent identity: BBa J63010
display id: BBa_J63010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein fusion vector (Silver lab standard)
creator: Ira Phillips and Caroline Ajo-Franklin
created: 2006-10-17T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: standard biofusion plasmid containing ccdB death gene and chlorenphenicol resistance
mutable notes: transform into TOP10 survival
mutable provenance: synthetized DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: beta-lactamase (bla gene)
sequence annotation: incorrect silver fusion prefix
sequence annotation: RNAI transcript
sequence annotation: f1 origin
sequence annotation: modified BioBrick suffix
sequence annotation: lacZ alpha peptide
sequence annotation: modified BioBrick prefix
sequence annotation: RNAII transcript
sequence annotation: rep pMB1 replication origin
sequence: https://synbiohub.org/public/igem/BBa_J63010_sequence/1

beta-lactamase (bla gene)
type: SequenceAnnotation
persistent identity: annotation1908165
display id: annotation1908165
version: 1
top level: V0120
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1908165/range1908165/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1908165/range1908165/1
type: Range
persistent identity: range1908165
display id: range1908165
version: 1
direction: direction/reverse
top level: V0120
start: 1253
end: 2113
orientation: inline

incorrect silver fusion prefix
type: SequenceAnnotation
persistent identity: annotation2172657
display id: annotation2172657
version: 1
top level: V0120
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J63010/annotation2172657/range2172657/1

https://synbiohub.org/public/igem/BBa_J63010/annotation2172657/range2172657/1
type: Range
persistent identity: range2172657
display id: range2172657
version: 1
direction: direction/forward
top level: V0120
start: 3260
end: 3260
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1969671
display id: annotation1969671
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969671/range1969671/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969671/range1969671/1
type: Range
persistent identity: range1969671
display id: range1969671
version: 1
direction: direction/forward
top level: V0120
start: 936
end: 1043
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1969668
display id: annotation1969668
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969668/range1969668/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969668/range1969668/1
type: Range
persistent identity: range1969668
display id: range1969668
version: 1
direction: direction/forward
top level: V0120
start: 2632
end: 2938
orientation: inline

modified BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1908166
display id: annotation1908166
version: 1
top level: V0120
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1908166/range1908166/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1908166/range1908166/1
type: Range
persistent identity: range1908166
display id: range1908166
version: 1
direction: direction/forward
top level: V0120
start: 1
end: 20
orientation: inline

lacZ alpha peptide
type: SequenceAnnotation
persistent identity: annotation1969669
display id: annotation1969669
version: 1
top level: V0120
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969669/range1969669/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969669/range1969669/1
type: Range
persistent identity: range1969669
display id: range1969669
version: 1
direction: direction/reverse
top level: V0120
start: 3028
end: 3187
orientation: inline

modified BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969672
display id: annotation1969672
version: 1
top level: V0120
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969672/range1969672/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969672/range1969672/1
type: Range
persistent identity: range1969672
display id: range1969672
version: 1
direction: direction/forward
top level: V0120
start: 3246
end: 3266
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1969670
display id: annotation1969670
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969670/range1969670/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969670/range1969670/1
type: Range
persistent identity: range1969670
display id: range1969670
version: 1
direction: direction/reverse
top level: V0120
start: 494
end: 1046
orientation: inline

rep pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation1908167
display id: annotation1908167
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1908167/range1908167/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1908167/range1908167/1
type: Range
persistent identity: range1908167
display id: range1908167
version: 1
direction: direction/forward
top level: V0120
start: 479
end: 1093
orientation: inline

BBa_K209498
type: ComponentDefinition
persistent identity: BBa K209498
display id: BBa_K209498
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCEP4
creator: Benjamin Rhau
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:29Z
discontinued: false
dominant: false
group u list: _312_
m user id: 0
owner id: 3698
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Commercial plasmid used to make BBa_K209432
mutable notes: Not a designed plasmid.
mutable provenance: Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K209498_sequence/1

BBa_J176125
type: ComponentDefinition
persistent identity: BBa J176125
display id: BBa_J176125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NLS-pcDNA3.1+
creator: Brady Laughlin
created: 2011-10-21T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: NLS-pcDNA3.1+, pcDNA3.1+ with Eco/Spe NLS BioBrick inserted into Eco/Xba; amp, neo
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: SV40 early polyA signal
sequence annotation: f1 origin
sequence annotation: X/S mixed site
sequence annotation: pUC origin
sequence annotation: T7 promoter priming
sequence annotation: source
sequence annotation: BGH polyadenylation sequence
sequence annotation: MCS
sequence annotation: NLS - T0100
sequence annotation: CMV promoter
sequence annotation: SV40 promoter/ origin
sequence annotation: AMP resistance gene
sequence annotation: Neomycin R
sequence annotation: BGH reverse priming
sequence annotation: MCS (continued)
sequence annotation: BB prefix
sequence: https://synbiohub.org/public/igem/BBa_J176125_sequence/1

SV40 early polyA signal
type: SequenceAnnotation
persistent identity: annotation2160202
display id: annotation2160202
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160202/range2160202/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160202/range2160202/1
type: Range
persistent identity: range2160202
display id: range2160202
version: 1
direction: direction/forward
top level: BBa_J176125
start: 3107
end: 3237
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2160200
display id: annotation2160200
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160200/range2160200/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160200/range2160200/1
type: Range
persistent identity: range2160200
display id: range2160200
version: 1
direction: direction/forward
top level: BBa_J176125
start: 1301
end: 1729
orientation: inline

X/S mixed site
type: SequenceAnnotation
persistent identity: annotation2160206
display id: annotation2160206
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160206/range2160206/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160206/range2160206/1
type: Range
persistent identity: range2160206
display id: range2160206
version: 1
direction: direction/forward
top level: BBa_J176125
start: 994
end: 999
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2160203
display id: annotation2160203
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160203/range2160203/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160203/range2160203/1
type: Range
persistent identity: range2160203
display id: range2160203
version: 1
direction: direction/reverse
top level: BBa_J176125
start: 3620
end: 4290
orientation: inline

T7 promoter priming
type: SequenceAnnotation
persistent identity: annotation2160196
display id: annotation2160196
version: 1
top level: BBa_J176125
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160196/range2160196/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160196/range2160196/1
type: Range
persistent identity: range2160196
display id: range2160196
version: 1
direction: direction/forward
top level: BBa_J176125
start: 863
end: 882
orientation: inline

source
type: SequenceAnnotation
persistent identity: annotation2160210
display id: annotation2160210
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160210/range2160210/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160210/range2160210/1
type: Range
persistent identity: range2160210
display id: range2160210
version: 1
direction: direction/forward
top level: BBa_J176125
start: 1
end: 5431
orientation: inline

BGH polyadenylation sequence
type: SequenceAnnotation
persistent identity: annotation2160199
display id: annotation2160199
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160199/range2160199/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160199/range2160199/1
type: Range
persistent identity: range2160199
display id: range2160199
version: 1
direction: direction/forward
top level: BBa_J176125
start: 1031
end: 1255
orientation: inline

MCS
type: SequenceAnnotation
persistent identity: annotation2160197
display id: annotation2160197
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160197/range2160197/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160197/range2160197/1
type: Range
persistent identity: range2160197
display id: range2160197
version: 1
direction: direction/forward
top level: BBa_J176125
start: 895
end: 951
orientation: inline

NLS - T0100
type: SequenceAnnotation
persistent identity: annotation2160205
display id: annotation2160205
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160205/range2160205/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160205/range2160205/1
type: Range
persistent identity: range2160205
display id: range2160205
version: 1
direction: direction/forward
top level: BBa_J176125
start: 973
end: 993
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2160195
display id: annotation2160195
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160195/range2160195/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160195/range2160195/1
type: Range
persistent identity: range2160195
display id: range2160195
version: 1
direction: direction/forward
top level: BBa_J176125
start: 232
end: 819
orientation: inline

SV40 promoter/ origin
type: SequenceAnnotation
persistent identity: annotation2160201
display id: annotation2160201
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160201/range2160201/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160201/range2160201/1
type: Range
persistent identity: range2160201
display id: range2160201
version: 1
direction: direction/forward
top level: BBa_J176125
start: 1734
end: 2077
orientation: inline

AMP resistance gene
type: SequenceAnnotation
persistent identity: annotation2160204
display id: annotation2160204
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160204/range2160204/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160204/range2160204/1
type: Range
persistent identity: range2160204
display id: range2160204
version: 1
direction: direction/reverse
top level: BBa_J176125
start: 4435
end: 5431
orientation: inline

Neomycin R
type: SequenceAnnotation
persistent identity: annotation2160209
display id: annotation2160209
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160209/range2160209/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160209/range2160209/1
type: Range
persistent identity: range2160209
display id: range2160209
version: 1
direction: direction/forward
top level: BBa_J176125
start: 2139
end: 2933
orientation: inline

BGH reverse priming
type: SequenceAnnotation
persistent identity: annotation2160198
display id: annotation2160198
version: 1
top level: BBa_J176125
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160198/range2160198/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160198/range2160198/1
type: Range
persistent identity: range2160198
display id: range2160198
version: 1
direction: direction/reverse
top level: BBa_J176125
start: 1025
end: 1042
orientation: inline

MCS (continued)
type: SequenceAnnotation
persistent identity: annotation2160207
display id: annotation2160207
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160207/range2160207/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160207/range2160207/1
type: Range
persistent identity: range2160207
display id: range2160207
version: 1
direction: direction/forward
top level: BBa_J176125
start: 1000
end: 1013
orientation: inline

BB prefix
type: SequenceAnnotation
persistent identity: annotation2160208
display id: annotation2160208
version: 1
top level: BBa_J176125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176125/annotation2160208/range2160208/1

https://synbiohub.org/public/igem/BBa_J176125/annotation2160208/range2160208/1
type: Range
persistent identity: range2160208
display id: range2160208
version: 1
direction: direction/forward
top level: BBa_J176125
start: 952
end: 972
orientation: inline

BBa_K1559011
type: ComponentDefinition
persistent identity: BBa K1559011
display id: BBa_K1559011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low-copy plasmid pTRC99A (trc promoter and lacI removed)
creator: Xiuqi (Rex) Xia
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1944_
m user id: 0
owner id: 23047
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Ampicillin resistance
sequence annotation: pGEX 3 primer
sequence annotation: BioBrick standard prefix
sequence annotation: pBR322 origin
sequence annotation: AmpR promoter
sequence annotation: pBAD rev primer/pTrcHis rev primer
sequence annotation: rrnB T1 terminator
sequence annotation: BioBrick standard suffix
sequence annotation: rrnB terminator
sequence annotation: rrnB t2 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1559011_sequence/1

Ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation2424712
display id: annotation2424712
version: 1
top level: BBa_K1559011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1559011/annotation2424712/range2424712/1

https://synbiohub.org/public/igem/BBa_K1559011/annotation2424712/range2424712/1
type: Range
persistent identity: range2424712
display id: range2424712
version: 1
direction: direction/forward
top level: BBa_K1559011
start: 509
end: 1369
orientation: inline

pGEX 3 primer
type: SequenceAnnotation
persistent identity: annotation2424714
display id: annotation2424714
version: 1
top level: BBa_K1559011
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1559011/annotation2424714/range2424714/1

https://synbiohub.org/public/igem/BBa_K1559011/annotation2424714/range2424714/1
type: Range
persistent identity: range2424714
display id: range2424714
version: 1
direction: direction/forward
top level: BBa_K1559011
start: 2540
end: 2562
orientation: inline

BioBrick standard prefix
type: SequenceAnnotation
persistent identity: annotation2424715
display id: annotation2424715
version: 1
top level: BBa_K1559011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1559011/annotation2424715/range2424715/1

https://synbiohub.org/public/igem/BBa_K1559011/annotation2424715/range2424715/1
type: Range
persistent identity: range2424715
display id: range2424715
version: 1
direction: direction/forward
top level: BBa_K1559011
start: 2563
end: 2584
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation2424713
display id: annotation2424713
version: 1
top level: BBa_K1559011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1559011/annotation2424713/range2424713/1

https://synbiohub.org/public/igem/BBa_K1559011/annotation2424713/range2424713/1
type: Range
persistent identity: range2424713
display id: range2424713
version: 1
direction: direction/forward
top level: BBa_K1559011
start: 1524
end: 2143
orientation: inline

AmpR promoter
type: SequenceAnnotation
persistent identity: annotation2424711
display id: annotation2424711
version: 1
top level: BBa_K1559011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1559011/annotation2424711/range2424711/1

https://synbiohub.org/public/igem/BBa_K1559011/annotation2424711/range2424711/1
type: Range
persistent identity: range2424711
display id: range2424711
version: 1
direction: direction/forward
top level: BBa_K1559011
start: 439
end: 467
orientation: inline

pBAD rev primer/pTrcHis rev primer
type: SequenceAnnotation
persistent identity: annotation2424705
display id: annotation2424705
version: 1
top level: BBa_K1559011
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1559011/annotation2424705/range2424705/1

https://synbiohub.org/public/igem/BBa_K1559011/annotation2424705/range2424705/1
type: Range
persistent identity: range2424705
display id: range2424705
version: 1
direction: direction/reverse
top level: BBa_K1559011
start: 22
end: 39
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2424709
display id: annotation2424709
version: 1
top level: BBa_K1559011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1559011/annotation2424709/range2424709/1

https://synbiohub.org/public/igem/BBa_K1559011/annotation2424709/range2424709/1
type: Range
persistent identity: range2424709
display id: range2424709
version: 1
direction: direction/forward
top level: BBa_K1559011
start: 195
end: 238
orientation: inline

BioBrick standard suffix
type: SequenceAnnotation
persistent identity: annotation2424704
display id: annotation2424704
version: 1
top level: BBa_K1559011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1559011/annotation2424704/range2424704/1

https://synbiohub.org/public/igem/BBa_K1559011/annotation2424704/range2424704/1
type: Range
persistent identity: range2424704
display id: range2424704
version: 1
direction: direction/forward
top level: BBa_K1559011
start: 1
end: 21
orientation: inline

rrnB terminator
type: SequenceAnnotation
persistent identity: annotation2424707
display id: annotation2424707
version: 1
top level: BBa_K1559011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1559011/annotation2424707/range2424707/1

https://synbiohub.org/public/igem/BBa_K1559011/annotation2424707/range2424707/1
type: Range
persistent identity: range2424707
display id: range2424707
version: 1
direction: direction/forward
top level: BBa_K1559011
start: 72
end: 229
orientation: inline

rrnB t2 terminator
type: SequenceAnnotation
persistent identity: annotation2424710
display id: annotation2424710
version: 1
top level: BBa_K1559011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1559011/annotation2424710/range2424710/1

https://synbiohub.org/public/igem/BBa_K1559011/annotation2424710/range2424710/1
type: Range
persistent identity: range2424710
display id: range2424710
version: 1
direction: direction/forward
top level: BBa_K1559011
start: 370
end: 397
orientation: inline

BBa_K801001
type: ComponentDefinition
persistent identity: BBa K801001
display id: BBa_K801001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM101 yeast shuttle vector with pTEF1 promoter
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF1 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
mutable notes: All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible
mutable provenance: Based on invitrogen's pYES2 plasmid. For mor information see the part information of pTUM100 (BBa_K801000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 2 micron (??) origin
sequence annotation: CYC1-Terminator
sequence annotation: URA3
sequence annotation: T7-Promoter
sequence annotation: TEF1-Promoter
sequence annotation: pUC-origin
sequence annotation: AmpR
sequence: https://synbiohub.org/public/igem/BBa_K801001_sequence/1

2 micron (??) origin
type: SequenceAnnotation
persistent identity: annotation2201773
display id: annotation2201773
version: 1
top level: BBa_K801001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201773/range2201773/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201773/range2201773/1
type: Range
persistent identity: range2201773
display id: range2201773
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 3777
end: 5248
orientation: inline

CYC1-Terminator
type: SequenceAnnotation
persistent identity: annotation2201769
display id: annotation2201769
version: 1
top level: BBa_K801001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201769/range2201769/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201769/range2201769/1
type: Range
persistent identity: range2201769
display id: range2201769
version: 1
direction: direction/forward
top level: BBa_K801001
start: 540
end: 787
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2201772
display id: annotation2201772
version: 1
top level: BBa_K801001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201772/range2201772/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201772/range2201772/1
type: Range
persistent identity: range2201772
display id: range2201772
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 2666
end: 3773
orientation: inline

T7-Promoter
type: SequenceAnnotation
persistent identity: annotation2201767
display id: annotation2201767
version: 1
top level: BBa_K801001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201767/range2201767/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201767/range2201767/1
type: Range
persistent identity: range2201767
display id: range2201767
version: 1
direction: direction/forward
top level: BBa_K801001
start: 4
end: 23
orientation: inline

TEF1-Promoter
type: SequenceAnnotation
persistent identity: annotation2201768
display id: annotation2201768
version: 1
top level: BBa_K801001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201768/range2201768/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201768/range2201768/1
type: Range
persistent identity: range2201768
display id: range2201768
version: 1
direction: direction/forward
top level: BBa_K801001
start: 58
end: 505
orientation: inline

pUC-origin
type: SequenceAnnotation
persistent identity: annotation2201770
display id: annotation2201770
version: 1
top level: BBa_K801001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201770/range2201770/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201770/range2201770/1
type: Range
persistent identity: range2201770
display id: range2201770
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 970
end: 1643
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2201771
display id: annotation2201771
version: 1
top level: BBa_K801001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201771/range2201771/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201771/range2201771/1
type: Range
persistent identity: range2201771
display id: range2201771
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 1788
end: 2647
orientation: inline

BBa_M36093
type: ComponentDefinition
persistent identity: BBa M36093
display id: BBa_M36093
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S. cerevisiae sensor test rig
creator: Joseph Shih
created: 2013-05-07T11:00:00Z
modified: 2015-05-08T01:14:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 10436
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: S. cerevisae test vector for sensors. Has a DasherGFP protein coding sequence which produces a GFP protein. Comes with high copy number origin, kanamycin resistance, and leucine yeast selection; can replace with low copy number origin if ordering from DNA 2.0
mutable notes: None
mutable provenance: Vector is based off of pJ1201-03c plasmid developed by DNA2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: KanR
sequence annotation: Pamp
sequence annotation: Pleu
sequence annotation: CYC1 polyA
sequence annotation: yeast Kozak
sequence annotation: Leu
sequence annotation: pUCori
sequence annotation: 2 um yeast ori
sequence annotation: DasherGFP
sequence annotation: student sensors go here
sequence annotation: Leu polyA
sequence: https://synbiohub.org/public/igem/BBa_M36093_sequence/1

KanR
type: SequenceAnnotation
persistent identity: annotation2217223
display id: annotation2217223
version: 1
top level: BBa_M36093
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M36093/annotation2217223/range2217223/1

https://synbiohub.org/public/igem/BBa_M36093/annotation2217223/range2217223/1
type: Range
persistent identity: range2217223
display id: range2217223
version: 1
direction: direction/forward
top level: BBa_M36093
start: 129
end: 938
orientation: inline

Pamp
type: SequenceAnnotation
persistent identity: annotation2217222
display id: annotation2217222
version: 1
top level: BBa_M36093
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_M36093/annotation2217222/range2217222/1

https://synbiohub.org/public/igem/BBa_M36093/annotation2217222/range2217222/1
type: Range
persistent identity: range2217222
display id: range2217222
version: 1
direction: direction/forward
top level: BBa_M36093
start: 1
end: 128
orientation: inline

Pleu
type: SequenceAnnotation
persistent identity: annotation2217228
display id: annotation2217228
version: 1
top level: BBa_M36093
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_M36093/annotation2217228/range2217228/1

https://synbiohub.org/public/igem/BBa_M36093/annotation2217228/range2217228/1
type: Range
persistent identity: range2217228
display id: range2217228
version: 1
direction: direction/forward
top level: BBa_M36093
start: 3155
end: 3654
orientation: inline

CYC1 polyA
type: SequenceAnnotation
persistent identity: annotation2217227
display id: annotation2217227
version: 1
top level: BBa_M36093
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_M36093/annotation2217227/range2217227/1

https://synbiohub.org/public/igem/BBa_M36093/annotation2217227/range2217227/1
type: Range
persistent identity: range2217227
display id: range2217227
version: 1
direction: direction/forward
top level: BBa_M36093
start: 2885
end: 3136
orientation: inline

yeast Kozak
type: SequenceAnnotation
persistent identity: annotation2329525
display id: annotation2329525
version: 1
top level: BBa_M36093
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_M36093/annotation2329525/range2329525/1

https://synbiohub.org/public/igem/BBa_M36093/annotation2329525/range2329525/1
type: Range
persistent identity: range2329525
display id: range2329525
version: 1
direction: direction/forward
top level: BBa_M36093
start: 2172
end: 2177
orientation: inline

Leu
type: SequenceAnnotation
persistent identity: annotation2217229
display id: annotation2217229
version: 1
top level: BBa_M36093
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M36093/annotation2217229/range2217229/1

https://synbiohub.org/public/igem/BBa_M36093/annotation2217229/range2217229/1
type: Range
persistent identity: range2217229
display id: range2217229
version: 1
direction: direction/forward
top level: BBa_M36093
start: 3655
end: 4749
orientation: inline

pUCori
type: SequenceAnnotation
persistent identity: annotation2217224
display id: annotation2217224
version: 1
top level: BBa_M36093
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36093/annotation2217224/range2217224/1

https://synbiohub.org/public/igem/BBa_M36093/annotation2217224/range2217224/1
type: Range
persistent identity: range2217224
display id: range2217224
version: 1
direction: direction/forward
top level: BBa_M36093
start: 939
end: 1742
orientation: inline

2 um yeast ori
type: SequenceAnnotation
persistent identity: annotation2217231
display id: annotation2217231
version: 1
top level: BBa_M36093
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36093/annotation2217231/range2217231/1

https://synbiohub.org/public/igem/BBa_M36093/annotation2217231/range2217231/1
type: Range
persistent identity: range2217231
display id: range2217231
version: 1
direction: direction/forward
top level: BBa_M36093
start: 4854
end: 6299
orientation: inline

DasherGFP
type: SequenceAnnotation
persistent identity: annotation2217226
display id: annotation2217226
version: 1
top level: BBa_M36093
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M36093/annotation2217226/range2217226/1

https://synbiohub.org/public/igem/BBa_M36093/annotation2217226/range2217226/1
type: Range
persistent identity: range2217226
display id: range2217226
version: 1
direction: direction/forward
top level: BBa_M36093
start: 2178
end: 2883
orientation: inline

student sensors go here
type: SequenceAnnotation
persistent identity: annotation2217225
display id: annotation2217225
version: 1
top level: BBa_M36093
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36093/annotation2217225/range2217225/1

https://synbiohub.org/public/igem/BBa_M36093/annotation2217225/range2217225/1
type: Range
persistent identity: range2217225
display id: range2217225
version: 1
direction: direction/forward
top level: BBa_M36093
start: 1757
end: 2171
orientation: inline

Leu polyA
type: SequenceAnnotation
persistent identity: annotation2217230
display id: annotation2217230
version: 1
top level: BBa_M36093
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_M36093/annotation2217230/range2217230/1

https://synbiohub.org/public/igem/BBa_M36093/annotation2217230/range2217230/1
type: Range
persistent identity: range2217230
display id: range2217230
version: 1
direction: direction/forward
top level: BBa_M36093
start: 4750
end: 4849
orientation: inline

BBa_J72204
type: ComponentDefinition
persistent identity: BBa J72204
display id: BBa_J72204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ampicillin resistant split R6K vector w/ O16 HAs and FRT-Kan-FRT
creator: Josh Kittleson
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:08:29Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ampicillin resistant split R6K vector with O16 homology and kanamycin resistance flanked by FRT sites. Requires expression of pir to replicate. Referred to internally as pBjk2648r
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72204_sequence/1

BBa_K289008
type: ComponentDefinition
persistent identity: BBa K289008
display id: BBa_K289008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PGBKT7(YEAST PLASMIDE BACKBONE)
creator: Nan Hou
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _393_
m user id: 0
owner id: 4781
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The pGBKT7 vevtor expresses proteins fused to amino acids of the GAL4 DNA binding domain(DNA-BD).In yeast ,fusion proteins are expressed at high levels from the constitutive ADH1 promoter ;transcription is terminated by the T7 and ADH1 transcription termination signials. pGBKT7 also contains the T7 promoter, a c-Myc epitope tag, and a MCS. pGBKT7 replicates autonomously in both E.coli and S.cerevisiae from the pUC and 2μ ori, respectively. The vector carries the Kan for selection in E.coli and the TRP1 nutritional marker for selection in yeast. Yeast strains containing pGBKT7 exhibit a higher transformation efficiency than strains carrying other DNA-BD domain vectors.
mutable notes: suitable host strains:dh5α DH10 & other general and S.cerevisiae host strains:AH109 Y187 Y190 selectable marker:trp1
mutable provenance: comercial
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: T7
sequence annotation: dna-bd vevtor insert screening
sequence annotation: T7 terminator
sequence: https://synbiohub.org/public/igem/BBa_K289008_sequence/1

T7
type: SequenceAnnotation
persistent identity: annotation2061852
display id: annotation2061852
version: 1
top level: BBa_K289008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K289008/annotation2061852/range2061852/1

https://synbiohub.org/public/igem/BBa_K289008/annotation2061852/range2061852/1
type: Range
persistent identity: range2061852
display id: range2061852
version: 1
direction: direction/forward
top level: BBa_K289008
start: 1212
end: 1235
orientation: inline

dna-bd vevtor insert screening
type: SequenceAnnotation
persistent identity: annotation2061854
display id: annotation2061854
version: 1
top level: BBa_K289008
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K289008/annotation2061854/range2061854/1

https://synbiohub.org/public/igem/BBa_K289008/annotation2061854/range2061854/1
type: Range
persistent identity: range2061854
display id: range2061854
version: 1
direction: direction/reverse
top level: BBa_K289008
start: 1469
end: 1491
orientation: inline

dna-bd vevtor insert screening
type: SequenceAnnotation
persistent identity: annotation2061851
display id: annotation2061851
version: 1
top level: BBa_K289008
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K289008/annotation2061851/range2061851/1

https://synbiohub.org/public/igem/BBa_K289008/annotation2061851/range2061851/1
type: Range
persistent identity: range2061851
display id: range2061851
version: 1
direction: direction/forward
top level: BBa_K289008
start: 1155
end: 1172
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2061855
display id: annotation2061855
version: 1
top level: BBa_K289008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K289008/annotation2061855/range2061855/1

https://synbiohub.org/public/igem/BBa_K289008/annotation2061855/range2061855/1
type: Range
persistent identity: range2061855
display id: range2061855
version: 1
direction: direction/forward
top level: BBa_K289008
start: 1342
end: 1373
orientation: inline

Adh1P Acce
type: ComponentDefinition
persistent identity: BBa K106006
display id: BBa_K106006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, Adh1P, Adh1t)
creator: Sergio Peisajovich
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is an acceptor vector for AarI cloning, with A and D ends. Cloning into this plasmid will put your parts under the control of a Adh1 promoter (Adh1P) and Adh1 terminator (Adh1t). For more information regarding AarI acceptor vectors, and AarI cloning in general, please visit the UCSF 2008 wiki.
mutable notes: The pRS315 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites.
mutable provenance: A modified pRS315 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Stops in all 3 frames
sequence annotation: T7 promoter
sequence annotation: Adh1 Promoter
sequence annotation: pBR322 origin
sequence annotation: ampicillin resistance marker
sequence annotation: f1 origin
sequence annotation: D overhang
sequence annotation: T3 promoter
sequence annotation: AarI AD MCS
sequence annotation: CEN ARS
sequence annotation: lac promoter
sequence annotation: Adh1 terminator
sequence annotation: A overhang
sequence annotation: LEU2 marker
sequence annotation: ampicillin resistance promoter
sequence annotation: ATG start codon
sequence annotation: lacZalpha reporter
sequence: https://synbiohub.org/public/igem/BBa_K106006_sequence/1

Stops in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999672
display id: annotation1999672
version: 1
top level: Adh1P Acce
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999672/range1999672/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999672/range1999672/1
type: Range
persistent identity: range1999672
display id: range1999672
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 11
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999674
display id: annotation1999674
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999674/range1999674/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999674/range1999674/1
type: Range
persistent identity: range1999674
display id: range1999674
version: 1
direction: direction/reverse
top level: Adh1P Acce
start: 224
end: 242
orientation: inline

Adh1 Promoter
type: SequenceAnnotation
persistent identity: annotation1986773
display id: annotation1986773
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986773/range1986773/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986773/range1986773/1
type: Range
persistent identity: range1986773
display id: range1986773
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 6132
end: 7646
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999678
display id: annotation1999678
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999678/range1999678/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999678/range1999678/1
type: Range
persistent identity: range1999678
display id: range1999678
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 5068
end: 5687
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1986805
display id: annotation1986805
version: 1
top level: Adh1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986805/range1986805/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986805/range1986805/1
type: Range
persistent identity: range1986805
display id: range1986805
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 4053
end: 4911
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999676
display id: annotation1999676
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999676/range1999676/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999676/range1999676/1
type: Range
persistent identity: range1999676
display id: range1999676
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 427
end: 733
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999682
display id: annotation1999682
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999682/range1999682/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999682/range1999682/1
type: Range
persistent identity: range1999682
display id: range1999682
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 1
end: 4
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999680
display id: annotation1999680
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999680/range1999680/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999680/range1999680/1
type: Range
persistent identity: range1999680
display id: range1999680
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 6095
end: 6114
orientation: inline

AarI AD MCS
type: SequenceAnnotation
persistent identity: annotation1986789
display id: annotation1986789
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986789/range1986789/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986789/range1986789/1
type: Range
persistent identity: range1986789
display id: range1986789
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 4309
end: 4385
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1986790
display id: annotation1986790
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986790/range1986790/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986790/range1986790/1
type: Range
persistent identity: range1986790
display id: range1986790
version: 1
direction: direction/reverse
top level: Adh1P Acce
start: 3403
end: 3921
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999679
display id: annotation1999679
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999679/range1999679/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999679/range1999679/1
type: Range
persistent identity: range1999679
display id: range1999679
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 5996
end: 6025
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1986788
display id: annotation1986788
version: 1
top level: Adh1P Acce
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986788/range1986788/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986788/range1986788/1
type: Range
persistent identity: range1986788
display id: range1986788
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 22
end: 215
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999681
display id: annotation1999681
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999681/range1999681/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999681/range1999681/1
type: Range
persistent identity: range1999681
display id: range1999681
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 7647
end: 7650
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1986791
display id: annotation1986791
version: 1
top level: Adh1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986791/range1986791/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986791/range1986791/1
type: Range
persistent identity: range1986791
display id: range1986791
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 1570
end: 2676
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999677
display id: annotation1999677
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999677/range1999677/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999677/range1999677/1
type: Range
persistent identity: range1999677
display id: range1999677
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 3983
end: 4011
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation1999673
display id: annotation1999673
version: 1
top level: Adh1P Acce
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999673/range1999673/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999673/range1999673/1
type: Range
persistent identity: range1999673
display id: range1999673
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 7638
end: 7640
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999675
display id: annotation1999675
version: 1
top level: Adh1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999675/range1999675/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999675/range1999675/1
type: Range
persistent identity: range1999675
display id: range1999675
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 246
end: 405
orientation: inline

BBa_K1323017
type: ComponentDefinition
persistent identity: BBa K1323017
display id: BBa_K1323017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. coli - Staphylococcus Shuttle Vector
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2015-05-08T01:09:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1698_
m user id: 0
owner id: 18368
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ORI
sequence annotation: Biobrick suffix
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: Chloramphenicol resistance
sequence annotation: Biobrick prefix
sequence annotation: oriV (Staphylococcal ORI from pSK41)
sequence annotation: end of terminator
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: BBa_J04450
sequence annotation: Stem loop
sequence annotation: Scar: BglII site used to insert parts
sequence annotation: Erythromycin resistance
sequence annotation: E. coli his operon terminator
sequence annotation: Scar: BamHI sites used to insert parts
sequence annotation: T0 Terminator
sequence annotation: rep (pMB1)
sequence: https://synbiohub.org/public/igem/BBa_K1323017_sequence/1

ORI
type: SequenceAnnotation
persistent identity: annotation2403400
display id: annotation2403400
version: 1
top level: BBa_K1323017
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403400/range2403400/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403400/range2403400/1
type: Range
persistent identity: range2403400
display id: range2403400
version: 1
direction: direction/forward
top level: BBa_K1323017
start: 3081
end: 3081
orientation: inline

Biobrick suffix
type: SequenceAnnotation
persistent identity: annotation2403137
display id: annotation2403137
version: 1
top level: BBa_K1323017
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403137/range2403137/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403137/range2403137/1
type: Range
persistent identity: range2403137
display id: range2403137
version: 1
direction: direction/forward
top level: BBa_K1323017
start: 1
end: 21
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation2403199
display id: annotation2403199
version: 1
top level: BBa_K1323017
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403199/range2403199/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403199/range2403199/1
type: Range
persistent identity: range2403199
display id: range2403199
version: 1
direction: direction/reverse
top level: BBa_K1323017
start: 157
end: 176
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2403402
display id: annotation2403402
version: 1
top level: BBa_K1323017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403402/range2403402/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403402/range2403402/1
type: Range
persistent identity: range2403402
display id: range2403402
version: 1
direction: direction/reverse
top level: BBa_K1323017
start: 3966
end: 4625
orientation: inline

Biobrick prefix
type: SequenceAnnotation
persistent identity: annotation2403423
display id: annotation2403423
version: 1
top level: BBa_K1323017
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403423/range2403423/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403423/range2403423/1
type: Range
persistent identity: range2403423
display id: range2403423
version: 1
direction: direction/forward
top level: BBa_K1323017
start: 4855
end: 4876
orientation: inline

oriV (Staphylococcal ORI from pSK41)
type: SequenceAnnotation
persistent identity: annotation2403225
display id: annotation2403225
version: 1
top level: BBa_K1323017
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403225/range2403225/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403225/range2403225/1
type: Range
persistent identity: range2403225
display id: range2403225
version: 1
direction: direction/reverse
top level: BBa_K1323017
start: 192
end: 2124
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2403413
display id: annotation2403413
version: 1
top level: BBa_K1323017
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403413/range2403413/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403413/range2403413/1
type: Range
persistent identity: range2403413
display id: range2403413
version: 1
direction: direction/reverse
top level: BBa_K1323017
start: 4814
end: 4817
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation2403403
display id: annotation2403403
version: 1
top level: BBa_K1323017
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403403/range2403403/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403403/range2403403/1
type: Range
persistent identity: range2403403
display id: range2403403
version: 1
direction: direction/forward
top level: BBa_K1323017
start: 4738
end: 4757
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2403446
display id: annotation2403446
version: 1
top level: BBa_K1323017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403446/range2403446/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403446/range2403446/1
type: Range
persistent identity: range2403446
display id: range2403446
version: 1
direction: direction/forward
top level: BBa_K1323017
start: 4877
end: 5945
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2403198
display id: annotation2403198
version: 1
top level: BBa_K1323017
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403198/range2403198/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403198/range2403198/1
type: Range
persistent identity: range2403198
display id: range2403198
version: 1
direction: direction/forward
top level: BBa_K1323017
start: 30
end: 64
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2403422
display id: annotation2403422
version: 1
top level: BBa_K1323017
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403422/range2403422/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403422/range2403422/1
type: Range
persistent identity: range2403422
display id: range2403422
version: 1
direction: direction/reverse
top level: BBa_K1323017
start: 4827
end: 4846
orientation: inline

Scar: BglII site used to insert parts
type: SequenceAnnotation
persistent identity: annotation2403378
display id: annotation2403378
version: 1
top level: BBa_K1323017
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403378/range2403378/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403378/range2403378/1
type: Range
persistent identity: range2403378
display id: range2403378
version: 1
direction: direction/reverse
top level: BBa_K1323017
start: 2978
end: 2983
orientation: inline

Erythromycin resistance
type: SequenceAnnotation
persistent identity: annotation2403226
display id: annotation2403226
version: 1
top level: BBa_K1323017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403226/range2403226/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403226/range2403226/1
type: Range
persistent identity: range2403226
display id: range2403226
version: 1
direction: direction/reverse
top level: BBa_K1323017
start: 2172
end: 2903
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2403161
display id: annotation2403161
version: 1
top level: BBa_K1323017
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403161/range2403161/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403161/range2403161/1
type: Range
persistent identity: range2403161
display id: range2403161
version: 1
direction: direction/forward
top level: BBa_K1323017
start: 22
end: 93
orientation: inline

Scar: BamHI sites used to insert parts
type: SequenceAnnotation
persistent identity: annotation2403200
display id: annotation2403200
version: 1
top level: BBa_K1323017
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403200/range2403200/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403200/range2403200/1
type: Range
persistent identity: range2403200
display id: range2403200
version: 1
direction: direction/forward
top level: BBa_K1323017
start: 178
end: 191
orientation: inline

T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2403401
display id: annotation2403401
version: 1
top level: BBa_K1323017
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403401/range2403401/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403401/range2403401/1
type: Range
persistent identity: range2403401
display id: range2403401
version: 1
direction: direction/forward
top level: BBa_K1323017
start: 3847
end: 3952
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2403391
display id: annotation2403391
version: 1
top level: BBa_K1323017
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1323017/annotation2403391/range2403391/1

https://synbiohub.org/public/igem/BBa_K1323017/annotation2403391/range2403391/1
type: Range
persistent identity: range2403391
display id: range2403391
version: 1
direction: direction/reverse
top level: BBa_K1323017
start: 3066
end: 3680
orientation: inline

BBa_J176129
type: ComponentDefinition
persistent identity: BBa J176129
display id: BBa_J176129
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcVN
creator: Brady Laughlin
created: 2011-10-25T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: VP64-NLS-pcDNA3.1+, pcDNA3.1+ with Eco/Spe NLS BioBrick inserted into Eco/Xba; amp, neo
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: f1 origin
sequence annotation: CMV promoter
sequence annotation: BGH reverse priming
sequence annotation: NLS - T0100
sequence annotation: MCS (continued
sequence annotation: Neomycin R
sequence annotation: X/S mixed site
sequence annotation: T7 promoter priming
sequence annotation: MCS
sequence annotation: pUC origin
sequence annotation: source
sequence annotation: BB prefix
sequence annotation: SV40 early polyA signal
sequence annotation: BGH polyadenylation sequence
sequence annotation: SV40 promoter/origin
sequence annotation: VP64
sequence annotation: Amp resistance gene
sequence: https://synbiohub.org/public/igem/BBa_J176129_sequence/1

f1 origin
type: SequenceAnnotation
persistent identity: annotation2160650
display id: annotation2160650
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160650/range2160650/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160650/range2160650/1
type: Range
persistent identity: range2160650
display id: range2160650
version: 1
direction: direction/forward
top level: BBa_J176129
start: 1457
end: 1885
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2160645
display id: annotation2160645
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160645/range2160645/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160645/range2160645/1
type: Range
persistent identity: range2160645
display id: range2160645
version: 1
direction: direction/forward
top level: BBa_J176129
start: 232
end: 819
orientation: inline

BGH reverse priming
type: SequenceAnnotation
persistent identity: annotation2160648
display id: annotation2160648
version: 1
top level: BBa_J176129
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160648/range2160648/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160648/range2160648/1
type: Range
persistent identity: range2160648
display id: range2160648
version: 1
direction: direction/reverse
top level: BBa_J176129
start: 1181
end: 1198
orientation: inline

NLS - T0100
type: SequenceAnnotation
persistent identity: annotation2160655
display id: annotation2160655
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160655/range2160655/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160655/range2160655/1
type: Range
persistent identity: range2160655
display id: range2160655
version: 1
direction: direction/forward
top level: BBa_J176129
start: 1129
end: 1149
orientation: inline

MCS (continued
type: SequenceAnnotation
persistent identity: annotation2160657
display id: annotation2160657
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160657/range2160657/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160657/range2160657/1
type: Range
persistent identity: range2160657
display id: range2160657
version: 1
direction: direction/forward
top level: BBa_J176129
start: 1156
end: 1169
orientation: inline

Neomycin R
type: SequenceAnnotation
persistent identity: annotation2160659
display id: annotation2160659
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160659/range2160659/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160659/range2160659/1
type: Range
persistent identity: range2160659
display id: range2160659
version: 1
direction: direction/forward
top level: BBa_J176129
start: 2295
end: 3089
orientation: inline

X/S mixed site
type: SequenceAnnotation
persistent identity: annotation2160656
display id: annotation2160656
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160656/range2160656/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160656/range2160656/1
type: Range
persistent identity: range2160656
display id: range2160656
version: 1
direction: direction/forward
top level: BBa_J176129
start: 1150
end: 1155
orientation: inline

T7 promoter priming
type: SequenceAnnotation
persistent identity: annotation2160646
display id: annotation2160646
version: 1
top level: BBa_J176129
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160646/range2160646/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160646/range2160646/1
type: Range
persistent identity: range2160646
display id: range2160646
version: 1
direction: direction/forward
top level: BBa_J176129
start: 863
end: 882
orientation: inline

MCS
type: SequenceAnnotation
persistent identity: annotation2160647
display id: annotation2160647
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160647/range2160647/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160647/range2160647/1
type: Range
persistent identity: range2160647
display id: range2160647
version: 1
direction: direction/forward
top level: BBa_J176129
start: 895
end: 951
orientation: inline

X/S mixed site
type: SequenceAnnotation
persistent identity: annotation2160661
display id: annotation2160661
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160661/range2160661/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160661/range2160661/1
type: Range
persistent identity: range2160661
display id: range2160661
version: 1
direction: direction/forward
top level: BBa_J176129
start: 1123
end: 1128
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2160653
display id: annotation2160653
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160653/range2160653/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160653/range2160653/1
type: Range
persistent identity: range2160653
display id: range2160653
version: 1
direction: direction/reverse
top level: BBa_J176129
start: 3776
end: 4446
orientation: inline

source
type: SequenceAnnotation
persistent identity: annotation2160662
display id: annotation2160662
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160662/range2160662/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160662/range2160662/1
type: Range
persistent identity: range2160662
display id: range2160662
version: 1
direction: direction/forward
top level: BBa_J176129
start: 1
end: 5587
orientation: inline

BB prefix
type: SequenceAnnotation
persistent identity: annotation2160658
display id: annotation2160658
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160658/range2160658/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160658/range2160658/1
type: Range
persistent identity: range2160658
display id: range2160658
version: 1
direction: direction/forward
top level: BBa_J176129
start: 952
end: 972
orientation: inline

SV40 early polyA signal
type: SequenceAnnotation
persistent identity: annotation2160652
display id: annotation2160652
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160652/range2160652/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160652/range2160652/1
type: Range
persistent identity: range2160652
display id: range2160652
version: 1
direction: direction/forward
top level: BBa_J176129
start: 3263
end: 3393
orientation: inline

BGH polyadenylation sequence
type: SequenceAnnotation
persistent identity: annotation2160649
display id: annotation2160649
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160649/range2160649/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160649/range2160649/1
type: Range
persistent identity: range2160649
display id: range2160649
version: 1
direction: direction/forward
top level: BBa_J176129
start: 1187
end: 1411
orientation: inline

SV40 promoter/origin
type: SequenceAnnotation
persistent identity: annotation2160651
display id: annotation2160651
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160651/range2160651/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160651/range2160651/1
type: Range
persistent identity: range2160651
display id: range2160651
version: 1
direction: direction/forward
top level: BBa_J176129
start: 1890
end: 2233
orientation: inline

VP64
type: SequenceAnnotation
persistent identity: annotation2160660
display id: annotation2160660
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160660/range2160660/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160660/range2160660/1
type: Range
persistent identity: range2160660
display id: range2160660
version: 1
direction: direction/forward
top level: BBa_J176129
start: 973
end: 1122
orientation: inline

Amp resistance gene
type: SequenceAnnotation
persistent identity: annotation2160654
display id: annotation2160654
version: 1
top level: BBa_J176129
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J176129/annotation2160654/range2160654/1

https://synbiohub.org/public/igem/BBa_J176129/annotation2160654/range2160654/1
type: Range
persistent identity: range2160654
display id: range2160654
version: 1
direction: direction/reverse
top level: BBa_J176129
start: 4591
end: 5587
orientation: inline

BBa_J72218
type: ComponentDefinition
persistent identity: BBa J72218
display id: BBa_J72218
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kanamycin resistant p15A vector
creator: Josh Kittleson
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:08:29Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: kanamycin resistant p15A vector. referred to internally as pBjo1601K
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72218_sequence/1

BBa_K1341008
type: ComponentDefinition
persistent identity: BBa K1341008
display id: BBa_K1341008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pUC57 Plasmid_Backbone
creator: Run-ze Zhang
created: 2014-09-30T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
group u list: _1716_
m user id: 0
owner id: 18362
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pUC57 Plasmid_Backbone
mutable notes: pUC57 Plasmid_Backbone
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/Y14837.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1341008_sequence/1

pSB1A3 lac
type: ComponentDefinition
persistent identity: BBa K627015
display id: BBa_K627015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid backbone pSB1A3 carrying the lac operon induction system
creator: iGEM11 Potsdam_Bioware
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _799_
m user id: 0
owner id: 10585
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

Eukaryotic
type: ComponentDefinition
persistent identity: BBa K157040
display id: BBa_K157040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Eukaryotic terminator vector (very similar to pSB1AK8)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Transfection vector with CMV Promotor
mutable notes: none
mutable provenance: BBa_J52017 and CMV Promotor
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: terminator
sequence annotation: kanamycin resistance marker
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence annotation: rep pMB(1)
sequence annotation: BioBrick prefix partial (missing G at 3' end)
sequence annotation: BioBrick suffix
sequence: https://synbiohub.org/public/igem/BBa_K157040_sequence/1

terminator
type: SequenceAnnotation
persistent identity: annotation1999641
display id: annotation1999641
version: 1
top level: Eukaryotic
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1999641/range1999641/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1999641/range1999641/1
type: Range
persistent identity: range1999641
display id: range1999641
version: 1
direction: direction/forward
top level: Eukaryotic
start: 22
end: 259
orientation: inline

kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation1999643
display id: annotation1999643
version: 1
top level: Eukaryotic
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1999643/range1999643/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1999643/range1999643/1
type: Range
persistent identity: range1999643
display id: range1999643
version: 1
direction: direction/reverse
top level: Eukaryotic
start: 1334
end: 2149
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1999644
display id: annotation1999644
version: 1
top level: Eukaryotic
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1999644/range1999644/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1999644/range1999644/1
type: Range
persistent identity: range1999644
display id: range1999644
version: 1
direction: direction/reverse
top level: Eukaryotic
start: 2304
end: 3164
orientation: inline

rep pMB(1)
type: SequenceAnnotation
persistent identity: annotation1999642
display id: annotation1999642
version: 1
top level: Eukaryotic
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1999642/range1999642/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1999642/range1999642/1
type: Range
persistent identity: range1999642
display id: range1999642
version: 1
direction: direction/reverse
top level: Eukaryotic
start: 498
end: 1112
orientation: inline

BioBrick prefix partial (missing G at 3' end)
type: SequenceAnnotation
persistent identity: annotation1999652
display id: annotation1999652
version: 1
top level: Eukaryotic
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1999652/range1999652/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1999652/range1999652/1
type: Range
persistent identity: range1999652
display id: range1999652
version: 1
direction: direction/forward
top level: Eukaryotic
start: 3405
end: 3425
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1992676
display id: annotation1992676
version: 1
top level: Eukaryotic
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1992676/range1992676/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1992676/range1992676/1
type: Range
persistent identity: range1992676
display id: range1992676
version: 1
direction: direction/forward
top level: Eukaryotic
start: 1
end: 21
orientation: inline

pSB6C5
type: ComponentDefinition
persistent identity: pSB6C5
display id: pSB6C5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBR322 Derived Chloramphenicol Backbone
creator: Jonathan Babb
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _525_
m user id: 0
owner id: 5564
owning group id: 525
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Made by the iGEM 2010 MIT team
mutable notes: To be entered
mutable provenance: To be entered
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pMB1 Ori
sequence annotation: VF2
sequence annotation: ROP
sequence annotation: Biobrick Suffix
sequence annotation: Biobrick Prefix
sequence annotation: P1004 (Cm Resistance)
sequence annotation: VR
sequence annotation: Deleted Region / BamHI site
sequence: https://synbiohub.org/public/igem/pSB6C5_sequence/1

pMB1 Ori
type: SequenceAnnotation
persistent identity: annotation2112143
display id: annotation2112143
version: 1
top level: pSB6C5
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6C5/annotation2112143/range2112143/1

https://synbiohub.org/public/igem/pSB6C5/annotation2112143/range2112143/1
type: Range
persistent identity: range2112143
display id: range2112143
version: 1
direction: direction/forward
top level: pSB6C5
start: 1513
end: 2127
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2112184
display id: annotation2112184
version: 1
top level: pSB6C5
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB6C5/annotation2112184/range2112184/1

https://synbiohub.org/public/igem/pSB6C5/annotation2112184/range2112184/1
type: Range
persistent identity: range2112184
display id: range2112184
version: 1
direction: direction/forward
top level: pSB6C5
start: 2212
end: 2231
orientation: inline

ROP
type: SequenceAnnotation
persistent identity: annotation2112142
display id: annotation2112142
version: 1
top level: pSB6C5
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6C5/annotation2112142/range2112142/1

https://synbiohub.org/public/igem/pSB6C5/annotation2112142/range2112142/1
type: Range
persistent identity: range2112142
display id: range2112142
version: 1
direction: direction/forward
top level: pSB6C5
start: 979
end: 1172
orientation: inline

Biobrick Suffix
type: SequenceAnnotation
persistent identity: annotation2112127
display id: annotation2112127
version: 1
top level: pSB6C5
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6C5/annotation2112127/range2112127/1

https://synbiohub.org/public/igem/pSB6C5/annotation2112127/range2112127/1
type: Range
persistent identity: range2112127
display id: range2112127
version: 1
direction: direction/forward
top level: pSB6C5
start: 1
end: 21
orientation: inline

Biobrick Prefix
type: SequenceAnnotation
persistent identity: annotation2112126
display id: annotation2112126
version: 1
top level: pSB6C5
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6C5/annotation2112126/range2112126/1

https://synbiohub.org/public/igem/pSB6C5/annotation2112126/range2112126/1
type: Range
persistent identity: range2112126
display id: range2112126
version: 1
direction: direction/forward
top level: pSB6C5
start: 2335
end: 2356
orientation: inline

P1004 (Cm Resistance)
type: SequenceAnnotation
persistent identity: annotation2112128
display id: annotation2112128
version: 1
top level: pSB6C5
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6C5/annotation2112128/range2112128/1

https://synbiohub.org/public/igem/pSB6C5/annotation2112128/range2112128/1
type: Range
persistent identity: range2112128
display id: range2112128
version: 1
direction: direction/forward
top level: pSB6C5
start: 184
end: 952
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2112145
display id: annotation2112145
version: 1
top level: pSB6C5
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB6C5/annotation2112145/range2112145/1

https://synbiohub.org/public/igem/pSB6C5/annotation2112145/range2112145/1
type: Range
persistent identity: range2112145
display id: range2112145
version: 1
direction: direction/reverse
top level: pSB6C5
start: 116
end: 135
orientation: inline

Deleted Region / BamHI site
type: SequenceAnnotation
persistent identity: annotation2112144
display id: annotation2112144
version: 1
top level: pSB6C5
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6C5/annotation2112144/range2112144/1

https://synbiohub.org/public/igem/pSB6C5/annotation2112144/range2112144/1
type: Range
persistent identity: range2112144
display id: range2112144
version: 1
direction: direction/forward
top level: pSB6C5
start: 1479
end: 1484
orientation: inline

pSB1AK8
type: ComponentDefinition
persistent identity: pSB1AK8
display id: pSB1AK8
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Eukaryotic terminator vector (formerly BBa_J52017)
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This vector is made of BioBrick vector pSB1AK3, which has eucaryotic terminator added.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rep (pMB1)
sequence annotation: BioBrick suffix
sequence annotation: kanR
sequence annotation: terminator
sequence annotation: BioBrick prefix
sequence annotation: ampR
sequence: https://synbiohub.org/public/igem/pSB1AK8_sequence/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1999648
display id: annotation1999648
version: 1
top level: pSB1AK8
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999648/range1999648/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999648/range1999648/1
type: Range
persistent identity: range1999648
display id: range1999648
version: 1
direction: direction/reverse
top level: pSB1AK8
start: 498
end: 1112
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1999646
display id: annotation1999646
version: 1
top level: pSB1AK8
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999646/range1999646/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999646/range1999646/1
type: Range
persistent identity: range1999646
display id: range1999646
version: 1
direction: direction/forward
top level: pSB1AK8
start: 1
end: 21
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation1999649
display id: annotation1999649
version: 1
top level: pSB1AK8
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999649/range1999649/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999649/range1999649/1
type: Range
persistent identity: range1999649
display id: range1999649
version: 1
direction: direction/reverse
top level: pSB1AK8
start: 1334
end: 2149
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1999647
display id: annotation1999647
version: 1
top level: pSB1AK8
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999647/range1999647/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999647/range1999647/1
type: Range
persistent identity: range1999647
display id: range1999647
version: 1
direction: direction/forward
top level: pSB1AK8
start: 22
end: 259
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1999651
display id: annotation1999651
version: 1
top level: pSB1AK8
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999651/range1999651/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999651/range1999651/1
type: Range
persistent identity: range1999651
display id: range1999651
version: 1
direction: direction/forward
top level: pSB1AK8
start: 3405
end: 3426
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation1999650
display id: annotation1999650
version: 1
top level: pSB1AK8
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999650/range1999650/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999650/range1999650/1
type: Range
persistent identity: range1999650
display id: range1999650
version: 1
direction: direction/reverse
top level: pSB1AK8
start: 2304
end: 3164
orientation: inline

BBa_K590010
type: ComponentDefinition
persistent identity: BBa K590010
display id: BBa_K590010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGA1A3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert
creator: Michael Brasino, Alicia Wong, Rashmi Ravichandran
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Backbone that is Gibson reaction friendly (Bgl Brick)
mutable notes: sequence confirmed
mutable provenance: Made from Gibson reaction
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ampR
sequence annotation: end of terminator
sequence annotation: rep(pMB1)
sequence annotation: pGA Suffix
sequence annotation: ORI
sequence annotation: terminator stem loop
sequence annotation: Vf2
sequence annotation: E. coli his operon terminator
sequence annotation: Vr
sequence annotation: pGA Prefix
sequence: https://synbiohub.org/public/igem/BBa_K590010_sequence/1

ampR
type: SequenceAnnotation
persistent identity: annotation2143935
display id: annotation2143935
version: 1
top level: BBa_K590010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K590010/annotation2143935/range2143935/1

https://synbiohub.org/public/igem/BBa_K590010/annotation2143935/range2143935/1
type: Range
persistent identity: range2143935
display id: range2143935
version: 1
direction: direction/reverse
top level: BBa_K590010
start: 1035
end: 1895
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2143937
display id: annotation2143937
version: 1
top level: BBa_K590010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K590010/annotation2143937/range2143937/1

https://synbiohub.org/public/igem/BBa_K590010/annotation2143937/range2143937/1
type: Range
persistent identity: range2143937
display id: range2143937
version: 1
direction: direction/reverse
top level: BBa_K590010
start: 2094
end: 2097
orientation: inline

rep(pMB1)
type: SequenceAnnotation
persistent identity: annotation2143929
display id: annotation2143929
version: 1
top level: BBa_K590010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K590010/annotation2143929/range2143929/1

https://synbiohub.org/public/igem/BBa_K590010/annotation2143929/range2143929/1
type: Range
persistent identity: range2143929
display id: range2143929
version: 1
direction: direction/reverse
top level: BBa_K590010
start: 261
end: 875
orientation: inline

pGA Suffix
type: SequenceAnnotation
persistent identity: annotation2143915
display id: annotation2143915
version: 1
top level: BBa_K590010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K590010/annotation2143915/range2143915/1

https://synbiohub.org/public/igem/BBa_K590010/annotation2143915/range2143915/1
type: Range
persistent identity: range2143915
display id: range2143915
version: 1
direction: direction/forward
top level: BBa_K590010
start: 1
end: 21
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2143930
display id: annotation2143930
version: 1
top level: BBa_K590010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K590010/annotation2143930/range2143930/1

https://synbiohub.org/public/igem/BBa_K590010/annotation2143930/range2143930/1
type: Range
persistent identity: range2143930
display id: range2143930
version: 1
direction: direction/forward
top level: BBa_K590010
start: 276
end: 276
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2143938
display id: annotation2143938
version: 1
top level: BBa_K590010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K590010/annotation2143938/range2143938/1

https://synbiohub.org/public/igem/BBa_K590010/annotation2143938/range2143938/1
type: Range
persistent identity: range2143938
display id: range2143938
version: 1
direction: direction/forward
top level: BBa_K590010
start: 2107
end: 2126
orientation: inline

Vf2
type: SequenceAnnotation
persistent identity: annotation2143936
display id: annotation2143936
version: 1
top level: BBa_K590010
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K590010/annotation2143936/range2143936/1

https://synbiohub.org/public/igem/BBa_K590010/annotation2143936/range2143936/1
type: Range
persistent identity: range2143936
display id: range2143936
version: 1
direction: direction/forward
top level: BBa_K590010
start: 2018
end: 2037
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2143921
display id: annotation2143921
version: 1
top level: BBa_K590010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K590010/annotation2143921/range2143921/1

https://synbiohub.org/public/igem/BBa_K590010/annotation2143921/range2143921/1
type: Range
persistent identity: range2143921
display id: range2143921
version: 1
direction: direction/forward
top level: BBa_K590010
start: 22
end: 93
orientation: inline

Vr
type: SequenceAnnotation
persistent identity: annotation2143924
display id: annotation2143924
version: 1
top level: BBa_K590010
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K590010/annotation2143924/range2143924/1

https://synbiohub.org/public/igem/BBa_K590010/annotation2143924/range2143924/1
type: Range
persistent identity: range2143924
display id: range2143924
version: 1
direction: direction/reverse
top level: BBa_K590010
start: 157
end: 176
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2143923
display id: annotation2143923
version: 1
top level: BBa_K590010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K590010/annotation2143923/range2143923/1

https://synbiohub.org/public/igem/BBa_K590010/annotation2143923/range2143923/1
type: Range
persistent identity: range2143923
display id: range2143923
version: 1
direction: direction/forward
top level: BBa_K590010
start: 30
end: 64
orientation: inline

pGA Prefix
type: SequenceAnnotation
persistent identity: annotation2143939
display id: annotation2143939
version: 1
top level: BBa_K590010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K590010/annotation2143939/range2143939/1

https://synbiohub.org/public/igem/BBa_K590010/annotation2143939/range2143939/1
type: Range
persistent identity: range2143939
display id: range2143939
version: 1
direction: direction/forward
top level: BBa_K590010
start: 2135
end: 2156
orientation: inline

BBa_K1256003
type: ComponentDefinition
persistent identity: BBa K1256003
display id: BBa_K1256003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3-Plac-SS-NB
creator: Pei-Hong Chen
created: 2014-06-08T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
group u list: _1614_
m user id: 0
owner id: 8995
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: TEST
mutable notes: TEST
mutable provenance: TEST
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BBa_E1010
sequence annotation: ori
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence annotation: Chloramphenicol resistance cassette
sequence annotation: OmpA Secretion Signal
sequence: https://synbiohub.org/public/igem/BBa_K1256003_sequence/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2378963
display id: annotation2378963
version: 1
top level: BBa_K1256003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1256003/annotation2378963/range2378963/1

https://synbiohub.org/public/igem/BBa_K1256003/annotation2378963/range2378963/1
type: Range
persistent identity: range2378963
display id: range2378963
version: 1
direction: direction/forward
top level: BBa_K1256003
start: 296
end: 1001
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2378966
display id: annotation2378966
version: 1
top level: BBa_K1256003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1256003/annotation2378966/range2378966/1

https://synbiohub.org/public/igem/BBa_K1256003/annotation2378966/range2378966/1
type: Range
persistent identity: range2378966
display id: range2378966
version: 1
direction: direction/forward
top level: BBa_K1256003
start: 1404
end: 2017
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2378962
display id: annotation2378962
version: 1
top level: BBa_K1256003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1256003/annotation2378962/range2378962/1

https://synbiohub.org/public/igem/BBa_K1256003/annotation2378962/range2378962/1
type: Range
persistent identity: range2378962
display id: range2378962
version: 1
direction: direction/forward
top level: BBa_K1256003
start: 209
end: 220
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2378965
display id: annotation2378965
version: 1
top level: BBa_K1256003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1256003/annotation2378965/range2378965/1

https://synbiohub.org/public/igem/BBa_K1256003/annotation2378965/range2378965/1
type: Range
persistent identity: range2378965
display id: range2378965
version: 1
direction: direction/forward
top level: BBa_K1256003
start: 1016
end: 1144
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2378961
display id: annotation2378961
version: 1
top level: BBa_K1256003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1256003/annotation2378961/range2378961/1

https://synbiohub.org/public/igem/BBa_K1256003/annotation2378961/range2378961/1
type: Range
persistent identity: range2378961
display id: range2378961
version: 1
direction: direction/forward
top level: BBa_K1256003
start: 1
end: 200
orientation: inline

Chloramphenicol resistance cassette
type: SequenceAnnotation
persistent identity: annotation2378967
display id: annotation2378967
version: 1
top level: BBa_K1256003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1256003/annotation2378967/range2378967/1

https://synbiohub.org/public/igem/BBa_K1256003/annotation2378967/range2378967/1
type: Range
persistent identity: range2378967
display id: range2378967
version: 1
direction: direction/reverse
top level: BBa_K1256003
start: 2304
end: 2963
orientation: inline

OmpA Secretion Signal
type: SequenceAnnotation
persistent identity: annotation2378964
display id: annotation2378964
version: 1
top level: BBa_K1256003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1256003/annotation2378964/range2378964/1

https://synbiohub.org/public/igem/BBa_K1256003/annotation2378964/range2378964/1
type: Range
persistent identity: range2378964
display id: range2378964
version: 1
direction: direction/forward
top level: BBa_K1256003
start: 227
end: 289
orientation: inline

BBa_K590014
type: ComponentDefinition
persistent identity: BBa K590014
display id: BBa_K590014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGA3K3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert
creator: Michael Brasino, Alicia Wong, Rashmi Ravichandran
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Backbone that is Gibson reaction friendly (Bgl Brick)
mutable notes: sequence confirmed
mutable provenance: Made from Gibson reaction
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: kanR
sequence annotation: Vr
sequence annotation: Vf2
sequence annotation: pGA suffix
sequence annotation: terminator stem loop
sequence annotation: pGA prefix
sequence annotation: E. coli his operon terminator
sequence annotation: p15A ori
sequence: https://synbiohub.org/public/igem/BBa_K590014_sequence/1

kanR
type: SequenceAnnotation
persistent identity: annotation2145073
display id: annotation2145073
version: 1
top level: BBa_K590014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K590014/annotation2145073/range2145073/1

https://synbiohub.org/public/igem/BBa_K590014/annotation2145073/range2145073/1
type: Range
persistent identity: range2145073
display id: range2145073
version: 1
direction: direction/reverse
top level: BBa_K590014
start: 239
end: 1054
orientation: inline

Vr
type: SequenceAnnotation
persistent identity: annotation2145072
display id: annotation2145072
version: 1
top level: BBa_K590014
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K590014/annotation2145072/range2145072/1

https://synbiohub.org/public/igem/BBa_K590014/annotation2145072/range2145072/1
type: Range
persistent identity: range2145072
display id: range2145072
version: 1
direction: direction/reverse
top level: BBa_K590014
start: 157
end: 176
orientation: inline

Vf2
type: SequenceAnnotation
persistent identity: annotation2145075
display id: annotation2145075
version: 1
top level: BBa_K590014
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K590014/annotation2145075/range2145075/1

https://synbiohub.org/public/igem/BBa_K590014/annotation2145075/range2145075/1
type: Range
persistent identity: range2145075
display id: range2145075
version: 1
direction: direction/forward
top level: BBa_K590014
start: 2611
end: 2630
orientation: inline

pGA suffix
type: SequenceAnnotation
persistent identity: annotation2145069
display id: annotation2145069
version: 1
top level: BBa_K590014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K590014/annotation2145069/range2145069/1

https://synbiohub.org/public/igem/BBa_K590014/annotation2145069/range2145069/1
type: Range
persistent identity: range2145069
display id: range2145069
version: 1
direction: direction/forward
top level: BBa_K590014
start: 1
end: 21
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2145076
display id: annotation2145076
version: 1
top level: BBa_K590014
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K590014/annotation2145076/range2145076/1

https://synbiohub.org/public/igem/BBa_K590014/annotation2145076/range2145076/1
type: Range
persistent identity: range2145076
display id: range2145076
version: 1
direction: direction/reverse
top level: BBa_K590014
start: 2700
end: 2719
orientation: inline

pGA prefix
type: SequenceAnnotation
persistent identity: annotation2145077
display id: annotation2145077
version: 1
top level: BBa_K590014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K590014/annotation2145077/range2145077/1

https://synbiohub.org/public/igem/BBa_K590014/annotation2145077/range2145077/1
type: Range
persistent identity: range2145077
display id: range2145077
version: 1
direction: direction/forward
top level: BBa_K590014
start: 2729
end: 2749
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2145071
display id: annotation2145071
version: 1
top level: BBa_K590014
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K590014/annotation2145071/range2145071/1

https://synbiohub.org/public/igem/BBa_K590014/annotation2145071/range2145071/1
type: Range
persistent identity: range2145071
display id: range2145071
version: 1
direction: direction/forward
top level: BBa_K590014
start: 30
end: 64
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2145070
display id: annotation2145070
version: 1
top level: BBa_K590014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K590014/annotation2145070/range2145070/1

https://synbiohub.org/public/igem/BBa_K590014/annotation2145070/range2145070/1
type: Range
persistent identity: range2145070
display id: range2145070
version: 1
direction: direction/forward
top level: BBa_K590014
start: 22
end: 93
orientation: inline

p15A ori
type: SequenceAnnotation
persistent identity: annotation2145074
display id: annotation2145074
version: 1
top level: BBa_K590014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K590014/annotation2145074/range2145074/1

https://synbiohub.org/public/igem/BBa_K590014/annotation2145074/range2145074/1
type: Range
persistent identity: range2145074
display id: range2145074
version: 1
direction: direction/reverse
top level: BBa_K590014
start: 1386
end: 2210
orientation: inline

BBa_I750017
type: ComponentDefinition
persistent identity: BBa I750017
display id: BBa_I750017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EcoRI-GenR-XbaI on pSB1AK3 backbone
creator: Phillip Dodson
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _132_
m user id: 0
owner id: 1721
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part was created from BBa_J61035 when it was cut with EcoRI and SpeI and this section inserted before a terminator BBa_B0015 on a pSB1AK3 plasmid. The part was never created in the pure form since when created it carried our part of interest between Xbal and SpeI. Creating it allows multiple parts to be shipped as a parts in this plasmid without further complicating explanations. This should perform a usefull service since many expression parts require the addition of RBS and terminator which is conveniently done using this sequence of events due to the multiple antibiotic resistances collected during the process.
mutable notes: none
mutable provenance: Part from kit provided BBa_J61035 and BBa_B0015.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Stem loop
sequence annotation: Verfication forward (VF2) primer binding site
sequence annotation: BioBrick suffix
sequence annotation: R6Kg origin
sequence annotation: NotI
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: EcoRI
sequence annotation: XbaI
sequence annotation: pMB1 origin
sequence annotation: beta-lactamase ampicillin resistance marker
sequence annotation: End of stem loop
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: kanamycin resistance
sequence annotation: E. coli his operon terminator
sequence annotation: Deletion of AC
sequence annotation: gentamycin resistance marker
sequence: https://synbiohub.org/public/igem/BBa_I750017_sequence/1

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975968
display id: annotation1975968
version: 1
top level: BBa_I750017
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975968/range1975968/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975968/range1975968/1
type: Range
persistent identity: range1975968
display id: range1975968
version: 1
direction: direction/reverse
top level: BBa_I750017
start: 3139
end: 3158
orientation: inline

Verfication forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975967
display id: annotation1975967
version: 1
top level: BBa_I750017
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975967/range1975967/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975967/range1975967/1
type: Range
persistent identity: range1975967
display id: range1975967
version: 1
direction: direction/forward
top level: BBa_I750017
start: 3050
end: 3069
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975963
display id: annotation1975963
version: 1
top level: BBa_I750017
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975963/range1975963/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975963/range1975963/1
type: Range
persistent identity: range1975963
display id: range1975963
version: 1
direction: direction/forward
top level: BBa_I750017
start: 1
end: 21
orientation: inline

R6Kg origin
type: SequenceAnnotation
persistent identity: annotation1952592
display id: annotation1952592
version: 1
top level: BBa_I750017
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1952592/range1952592/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1952592/range1952592/1
type: Range
persistent identity: range1952592
display id: range1952592
version: 1
direction: direction/forward
top level: BBa_I750017
start: 3201
end: 3584
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975965
display id: annotation1975965
version: 1
top level: BBa_I750017
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975965/range1975965/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975965/range1975965/1
type: Range
persistent identity: range1975965
display id: range1975965
version: 1
direction: direction/forward
top level: BBa_I750017
start: 3624
end: 3631
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1952590
display id: annotation1952590
version: 1
top level: BBa_I750017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1952590/range1952590/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1952590/range1952590/1
type: Range
persistent identity: range1952590
display id: range1952590
version: 1
direction: direction/reverse
top level: BBa_I750017
start: 2969
end: 2997
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975964
display id: annotation1975964
version: 1
top level: BBa_I750017
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975964/range1975964/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975964/range1975964/1
type: Range
persistent identity: range1975964
display id: range1975964
version: 1
direction: direction/forward
top level: BBa_I750017
start: 3168
end: 3173
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975966
display id: annotation1975966
version: 1
top level: BBa_I750017
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975966/range1975966/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975966/range1975966/1
type: Range
persistent identity: range1975966
display id: range1975966
version: 1
direction: direction/forward
top level: BBa_I750017
start: 4643
end: 4648
orientation: inline

pMB1 origin
type: SequenceAnnotation
persistent identity: annotation1975962
display id: annotation1975962
version: 1
top level: BBa_I750017
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975962/range1975962/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975962/range1975962/1
type: Range
persistent identity: range1975962
display id: range1975962
version: 1
direction: direction/reverse
top level: BBa_I750017
start: 261
end: 875
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975971
display id: annotation1975971
version: 1
top level: BBa_I750017
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975971/range1975971/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975971/range1975971/1
type: Range
persistent identity: range1975971
display id: range1975971
version: 1
direction: direction/forward
top level: BBa_I750017
start: 30
end: 64
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1952593
display id: annotation1952593
version: 1
top level: BBa_I750017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1952593/range1952593/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1952593/range1952593/1
type: Range
persistent identity: range1952593
display id: range1952593
version: 1
direction: direction/reverse
top level: BBa_I750017
start: 2067
end: 2927
orientation: inline

End of stem loop
type: SequenceAnnotation
persistent identity: annotation1975969
display id: annotation1975969
version: 1
top level: BBa_I750017
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975969/range1975969/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975969/range1975969/1
type: Range
persistent identity: range1975969
display id: range1975969
version: 1
direction: direction/reverse
top level: BBa_I750017
start: 3126
end: 3129
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975973
display id: annotation1975973
version: 1
top level: BBa_I750017
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975973/range1975973/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975973/range1975973/1
type: Range
persistent identity: range1975973
display id: range1975973
version: 1
direction: direction/reverse
top level: BBa_I750017
start: 157
end: 176
orientation: inline

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation1952594
display id: annotation1952594
version: 1
top level: BBa_I750017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1952594/range1952594/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1952594/range1952594/1
type: Range
persistent identity: range1952594
display id: range1952594
version: 1
direction: direction/reverse
top level: BBa_I750017
start: 1097
end: 1912
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1975970
display id: annotation1975970
version: 1
top level: BBa_I750017
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975970/range1975970/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975970/range1975970/1
type: Range
persistent identity: range1975970
display id: range1975970
version: 1
direction: direction/forward
top level: BBa_I750017
start: 22
end: 93
orientation: inline

Deletion of AC
type: SequenceAnnotation
persistent identity: annotation1975972
display id: annotation1975972
version: 1
top level: BBa_I750017
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1975972/range1975972/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1975972/range1975972/1
type: Range
persistent identity: range1975972
display id: range1975972
version: 1
direction: direction/forward
top level: BBa_I750017
start: 34
end: 35
orientation: inline

gentamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation1952591
display id: annotation1952591
version: 1
top level: BBa_I750017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I750017/annotation1952591/range1952591/1

https://synbiohub.org/public/igem/BBa_I750017/annotation1952591/range1952591/1
type: Range
persistent identity: range1952591
display id: range1952591
version: 1
direction: direction/reverse
top level: BBa_I750017
start: 3645
end: 4178
orientation: inline

BBa_K1680026
type: ComponentDefinition
persistent identity: BBa K1680026
display id: BBa_K1680026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pETUE
creator: Katharina Sporbeck
created: 2015-09-17T11:00:00Z
modified: 2015-10-28T03:58:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Expression Plasmid coding for His-tag, also containing a thrombine cleavage site between coding region and Histag, so that His-tag can be cleaved off. Vecotr opening with PstI/XmaI, clone part into it with PstI/NgoMIV. Ampicillin resistance.
mutable notes: very strong RBS 6xHis-Tag Thromin-cleavage-site NgoMIV-compatible restriction site for in-frame insertion
mutable provenance: pET-Vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: tet promoter
sequence annotation: T7 promoter
sequence annotation: RBS.1 strong
sequence annotation: rrnB T1 terminator
sequence annotation: 6x His-tag
sequence annotation: Lac operator
sequence annotation: T7 terminator
sequence annotation: ori
sequence annotation: AmpR
sequence annotation: lac operator
sequence annotation: f1 ori
sequence annotation: thrombin cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K1680026_sequence/1

tet promoter
type: SequenceAnnotation
persistent identity: annotation2472238
display id: annotation2472238
version: 1
top level: BBa_K1680026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472238/range2472238/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472238/range2472238/1
type: Range
persistent identity: range2472238
display id: range2472238
version: 1
direction: direction/reverse
top level: BBa_K1680026
start: 212
end: 240
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2472193
display id: annotation2472193
version: 1
top level: BBa_K1680026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472193/range2472193/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472193/range2472193/1
type: Range
persistent identity: range2472193
display id: range2472193
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 1
end: 19
orientation: inline

RBS.1 strong
type: SequenceAnnotation
persistent identity: annotation2472195
display id: annotation2472195
version: 1
top level: BBa_K1680026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472195/range2472195/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472195/range2472195/1
type: Range
persistent identity: range2472195
display id: range2472195
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 53
end: 67
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2472239
display id: annotation2472239
version: 1
top level: BBa_K1680026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472239/range2472239/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472239/range2472239/1
type: Range
persistent identity: range2472239
display id: range2472239
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 410
end: 496
orientation: inline

6x His-tag
type: SequenceAnnotation
persistent identity: annotation2472216
display id: annotation2472216
version: 1
top level: BBa_K1680026
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472216/range2472216/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472216/range2472216/1
type: Range
persistent identity: range2472216
display id: range2472216
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 77
end: 94
orientation: inline

Lac operator
type: SequenceAnnotation
persistent identity: annotation2472194
display id: annotation2472194
version: 1
top level: BBa_K1680026
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472194/range2472194/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472194/range2472194/1
type: Range
persistent identity: range2472194
display id: range2472194
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 20
end: 44
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2472260
display id: annotation2472260
version: 1
top level: BBa_K1680026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472260/range2472260/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472260/range2472260/1
type: Range
persistent identity: range2472260
display id: range2472260
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 683
end: 730
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2472305
display id: annotation2472305
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472305/range2472305/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472305/range2472305/1
type: Range
persistent identity: range2472305
display id: range2472305
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 2290
end: 2878
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2472289
display id: annotation2472289
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472289/range2472289/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472289/range2472289/1
type: Range
persistent identity: range2472289
display id: range2472289
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 1337
end: 2197
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2472328
display id: annotation2472328
version: 1
top level: BBa_K1680026
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472328/range2472328/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472328/range2472328/1
type: Range
persistent identity: range2472328
display id: range2472328
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 3277
end: 3296
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation2472261
display id: annotation2472261
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472261/range2472261/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472261/range2472261/1
type: Range
persistent identity: range2472261
display id: range2472261
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 767
end: 1222
orientation: inline

thrombin cleavage site
type: SequenceAnnotation
persistent identity: annotation2472237
display id: annotation2472237
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472237/range2472237/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472237/range2472237/1
type: Range
persistent identity: range2472237
display id: range2472237
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 104
end: 121
orientation: inline

BBa_K1621009
type: ComponentDefinition
persistent identity: BBa K1621009
display id: BBa_K1621009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pOP - protein expression vector compatible with RFC[25]
creator: Ramona Emig, Julika Neumann, Lara Stuehn
created: 2015-08-29T11:00:00Z
modified: 2015-09-01T01:44:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2038_
m user id: 25598
owner id: 25598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the sequence for a plasmid backbone that is designed for efficient overexpression of proteins in ''Escherichia coli''. Usually, vectors that are optimized for protein overexpression are low- to medium-copy plasmids. In contrast to cloning purposes, this is sufficient for protein expression. When a certain copy number is reached, the expression machinery of the cell is saturated and a further increase will not result in higher expression yields. High copy plasmids rather result in lower expression rates because more metabolic capacity is shared for replication. Many ''E. coli'' strains optimized for overexpression carry a chloramphenicol resistance gene as a selective marker. Plasmids carrying the same marker are not suitable for protein expression for two reasons. First, double selection for successfully transformed colonies on a medium with two antibiotics is not possible. Second, there is no selective pressure which makes it an advantage for the cell to even keep the plasmid. The iGEM standard vector pSB1C3 was designed for cloning purposes and thus is provided with an origin of replication yielding high plasmid copy numbers. Moreover, it carries a chloramphenicol resistance gene as selection marker which makes it a tough task to use this vector for expression purposes. Using a modified version of pSB1C3 (plasmid map pIG15_001) for overexpression resulted in an unsatisfying protein amount. Working with a common expression vector increased the overexpression efficiency significantly. Obviously, it is a lot of work to clone every part you want to send to the registry into an expression vector and additionally into pSB1C3 which exhibits a completely different cloning site. For those reasons, this new vector backbone was added to the iGEM registry which is suitable for efficient protein overexpression and compatible for cloning parts derived from the registry in a standardized procedure. The new vector, called pOP ??? plasmid for Overexpression of P…
mutable notes: The original sequence of pET22b+ was modified to make it compatible to iGEM standard cloning procedures. This includes the removal of recognition sites of several restriction enzymes as well as the insertion of RFC[25] as a multiple cloning site. Further details can be found on the main page.
mutable provenance: The sequence is a modified version of the common expression vector pET22b+. Five fragments that were needed for the assembly of the whole part were synthesized by Integrated DNA Technologies.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RBS
sequence annotation: T7 promoter
sequence annotation: BioBrick prefix RFC[25]
sequence annotation: ColE1 ori
sequence annotation: BioBrick suffix RFC[25]
sequence annotation: lacI
sequence annotation: VF2
sequence annotation: VR
sequence annotation: T7 terminator
sequence annotation: beta-lactamase
sequence: https://synbiohub.org/public/igem/BBa_K1621009_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2441409
display id: annotation2441409
version: 1
top level: BBa_K1621009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1621009/annotation2441409/range2441409/1

https://synbiohub.org/public/igem/BBa_K1621009/annotation2441409/range2441409/1
type: Range
persistent identity: range2441409
display id: range2441409
version: 1
direction: direction/forward
top level: BBa_K1621009
start: 4876
end: 4900
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2441408
display id: annotation2441408
version: 1
top level: BBa_K1621009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1621009/annotation2441408/range2441408/1

https://synbiohub.org/public/igem/BBa_K1621009/annotation2441408/range2441408/1
type: Range
persistent identity: range2441408
display id: range2441408
version: 1
direction: direction/forward
top level: BBa_K1621009
start: 4857
end: 4873
orientation: inline

BioBrick prefix RFC[25]
type: SequenceAnnotation
persistent identity: annotation2441402
display id: annotation2441402
version: 1
top level: BBa_K1621009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1621009/annotation2441402/range2441402/1

https://synbiohub.org/public/igem/BBa_K1621009/annotation2441402/range2441402/1
type: Range
persistent identity: range2441402
display id: range2441402
version: 1
direction: direction/forward
top level: BBa_K1621009
start: 4984
end: 5012
orientation: inline

ColE1 ori
type: SequenceAnnotation
persistent identity: annotation2441406
display id: annotation2441406
version: 1
top level: BBa_K1621009
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1621009/annotation2441406/range2441406/1

https://synbiohub.org/public/igem/BBa_K1621009/annotation2441406/range2441406/1
type: Range
persistent identity: range2441406
display id: range2441406
version: 1
direction: direction/forward
top level: BBa_K1621009
start: 1354
end: 1973
orientation: inline

BioBrick suffix RFC[25]
type: SequenceAnnotation
persistent identity: annotation2441401
display id: annotation2441401
version: 1
top level: BBa_K1621009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1621009/annotation2441401/range2441401/1

https://synbiohub.org/public/igem/BBa_K1621009/annotation2441401/range2441401/1
type: Range
persistent identity: range2441401
display id: range2441401
version: 1
direction: direction/forward
top level: BBa_K1621009
start: 1
end: 30
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2441407
display id: annotation2441407
version: 1
top level: BBa_K1621009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1621009/annotation2441407/range2441407/1

https://synbiohub.org/public/igem/BBa_K1621009/annotation2441407/range2441407/1
type: Range
persistent identity: range2441407
display id: range2441407
version: 1
direction: direction/reverse
top level: BBa_K1621009
start: 3391
end: 4470
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2441410
display id: annotation2441410
version: 1
top level: BBa_K1621009
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1621009/annotation2441410/range2441410/1

https://synbiohub.org/public/igem/BBa_K1621009/annotation2441410/range2441410/1
type: Range
persistent identity: range2441410
display id: range2441410
version: 1
direction: direction/forward
top level: BBa_K1621009
start: 4906
end: 4924
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2441404
display id: annotation2441404
version: 1
top level: BBa_K1621009
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1621009/annotation2441404/range2441404/1

https://synbiohub.org/public/igem/BBa_K1621009/annotation2441404/range2441404/1
type: Range
persistent identity: range2441404
display id: range2441404
version: 1
direction: direction/reverse
top level: BBa_K1621009
start: 159
end: 178
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2441403
display id: annotation2441403
version: 1
top level: BBa_K1621009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1621009/annotation2441403/range2441403/1

https://synbiohub.org/public/igem/BBa_K1621009/annotation2441403/range2441403/1
type: Range
persistent identity: range2441403
display id: range2441403
version: 1
direction: direction/forward
top level: BBa_K1621009
start: 105
end: 151
orientation: inline

beta-lactamase
type: SequenceAnnotation
persistent identity: annotation2441405
display id: annotation2441405
version: 1
top level: BBa_K1621009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1621009/annotation2441405/range2441405/1

https://synbiohub.org/public/igem/BBa_K1621009/annotation2441405/range2441405/1
type: Range
persistent identity: range2441405
display id: range2441405
version: 1
direction: direction/forward
top level: BBa_K1621009
start: 339
end: 1196
orientation: inline

BBa_J73999
type: ComponentDefinition
persistent identity: BBa J73999
display id: BBa_J73999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plasmid vector for Rhodobacter sphaeroides
creator: Junling Huo
created: 2009-12-10T12:00:00Z
modified: 2015-05-08T01:08:29Z
discontinued: false
dominant: false
group u list: _303_
m user id: 0
owner id: 3154
owning group id: 303
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This vector is based on pMG170/pMG171. LacZ promoter and lac reporter have been removed from original vector.
mutable notes: To make this vector more flexible and easier to control, we have deleted the lacZ promoter and lac reporter and made it a standard BioBrick vector.
mutable provenance: From pMG170/pMG171
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J73999_sequence/1

BBa_J18965
type: ComponentDefinition
persistent identity: BBa J18965
display id: BBa_J18965
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSS3K1
creator: Sean Sleight
created: 2013-06-17T11:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2480
owning group id: 172
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Vector with pSS Prefix A and Suffix B, confers kanamycin resistance, p15a origin of replication, constitutively expresses luxR and araC.
mutable notes: Inserted luxR and araC to be constitutively expressed in the reverse direction on the vector. Removed restriction sites in prefix and suffix to improve assembly success rate.
mutable provenance: Modified pGA3K3 from 2011 University of Washington iGEM team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J18965_sequence/1

BBa_K382004
type: ComponentDefinition
persistent identity: BBa K382004
display id: BBa_K382004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE Gus Reporter Series Vector with BiOBrick MCS (Kan resistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:18:58Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Basta resistance in plants. Has a constitutive plant promoter and in-frame Gus reporter. Conforms to Silver BioBrick Assembly Standard 21.
mutable notes: Silver Standard 21.
mutable provenance: Modified vector pORE_R from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K382004_sequence/1

BBa_J107055
type: ComponentDefinition
persistent identity: BBa J107055
display id: BBa_J107055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB3K3 with RBS-RFP-TT between SpeI-PstI
creator: Lorenzo Pasotti
created: 2011-08-11T11:00:00Z
modified: 2015-08-31T04:08:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _587_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: When the SpeI-PstI DNA fragment from BBa_J61002 , containing the mRFP1 gene with a strong RBS and a double terminator, is transferred into pSB3K3 the resulting plasmid is BBa_J107055 . See pSB3K3 for detailed feature information.
mutable notes: This is not a standard BioBrick plasmid, as the suffix is not SpeI-NotI-PstI.
mutable provenance: pSB3K3 (backbone) and BBa_J61002 (SpeI-PstI insert)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J107055_sequence/1

BBa_K209497
type: ComponentDefinition
persistent identity: BBa K209497
display id: BBa_K209497
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcDNA3.1(+)
creator: Benjamin Rhau
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:29Z
discontinued: false
dominant: false
group u list: _312_
m user id: 0
owner id: 3698
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Commercial plasmid used to make part BBa_K209473.
mutable notes: Not a designed plasmid.
mutable provenance: Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K209497_sequence/1

pSB1A2
type: ComponentDefinition
persistent identity: pSB1A2
display id: pSB1A2
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A2 (Replaced by pSB1A3)
creator: Tom Knight
created: 2004-05-25T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB1A2 is a high copy number plasmid carrying ampicillin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). pSB1A2 has one terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into any DNA outside the MCS. The orientation of the pBLA promoter driving the AmpR gene is such that relatively little transcription should be coming into the MCS from upstream. Ideally, a future version of the standard biobrick vectors would have terminators bracketing an MCS that were 100% efficient in terminating both into and out of the MCS region.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pBLA (rev)
sequence annotation: rep (pMB1) (rev)
sequence annotation: APr (rev)
sequence annotation: Suffix
sequence annotation: ORI
sequence annotation: VR
sequence annotation: Prefix
sequence annotation: VF2
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/pSB1A2_sequence/1

pBLA (rev)
type: SequenceAnnotation
persistent identity: annotation379265
display id: annotation379265
version: 1
top level: pSB1A2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A2/annotation379265/range379265/1

https://synbiohub.org/public/igem/pSB1A2/annotation379265/range379265/1
type: Range
persistent identity: range379265
display id: range379265
version: 1
direction: direction/reverse
top level: pSB1A2
start: 1852
end: 1852
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation379241
display id: annotation379241
version: 1
top level: pSB1A2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A2/annotation379241/range379241/1

https://synbiohub.org/public/igem/pSB1A2/annotation379241/range379241/1
type: Range
persistent identity: range379241
display id: range379241
version: 1
direction: direction/reverse
top level: pSB1A2
start: 183
end: 797
orientation: inline

APr (rev)
type: SequenceAnnotation
persistent identity: annotation379252
display id: annotation379252
version: 1
top level: pSB1A2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A2/annotation379252/range379252/1

https://synbiohub.org/public/igem/pSB1A2/annotation379252/range379252/1
type: Range
persistent identity: range379252
display id: range379252
version: 1
direction: direction/reverse
top level: pSB1A2
start: 957
end: 1817
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation379224
display id: annotation379224
version: 1
top level: pSB1A2
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A2/annotation379224/range379224/1

https://synbiohub.org/public/igem/pSB1A2/annotation379224/range379224/1
type: Range
persistent identity: range379224
display id: range379224
version: 1
direction: direction/forward
top level: pSB1A2
start: 1
end: 21
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation379236
display id: annotation379236
version: 1
top level: pSB1A2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A2/annotation379236/range379236/1

https://synbiohub.org/public/igem/pSB1A2/annotation379236/range379236/1
type: Range
persistent identity: range379236
display id: range379236
version: 1
direction: direction/forward
top level: pSB1A2
start: 198
end: 198
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation379233
display id: annotation379233
version: 1
top level: pSB1A2
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A2/annotation379233/range379233/1

https://synbiohub.org/public/igem/pSB1A2/annotation379233/range379233/1
type: Range
persistent identity: range379233
display id: range379233
version: 1
direction: direction/reverse
top level: pSB1A2
start: 79
end: 98
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1781822
display id: annotation1781822
version: 1
top level: pSB1A2
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A2/annotation1781822/range1781822/1

https://synbiohub.org/public/igem/pSB1A2/annotation1781822/range1781822/1
type: Range
persistent identity: range1781822
display id: range1781822
version: 1
direction: direction/forward
top level: pSB1A2
start: 2058
end: 2079
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation379274
display id: annotation379274
version: 1
top level: pSB1A2
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A2/annotation379274/range379274/1

https://synbiohub.org/public/igem/pSB1A2/annotation379274/range379274/1
type: Range
persistent identity: range379274
display id: range379274
version: 1
direction: direction/forward
top level: pSB1A2
start: 1940
end: 1959
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation379283
display id: annotation379283
version: 1
top level: pSB1A2
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A2/annotation379283/range379283/1

https://synbiohub.org/public/igem/pSB1A2/annotation379283/range379283/1
type: Range
persistent identity: range379283
display id: range379283
version: 1
direction: direction/forward
top level: pSB1A2
start: 2029
end: 2048
orientation: inline

lcp
type: ComponentDefinition
persistent identity: BBa I716101
display id: BBa_I716101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low Copy Ampicillin Resistance Plasmid
creator: David Tulga
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _110_
m user id: 0
owner id: 1694
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a Low Copy BioBrick 2.0 Plasmid with the Ampicillin Resistance Gene ampR.
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: This is modified and converted from the BioBrick 1.0 Plasmid pSB4A3.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RBS
sequence annotation: inverted repeat
sequence annotation: promoter region
sequence annotation: VF2 primer binding site
sequence annotation: direct repeat
sequence annotation: repA
sequence annotation: -10
sequence annotation: Berkeley suffix
sequence annotation: Berkeley prefix
sequence annotation: beta-lactamase promoter
sequence annotation: beta-lactamase ampicillin resistance gene
sequence annotation: -35
sequence annotation: AT rich region
sequence annotation: putative polyA
sequence annotation: par region
sequence: https://synbiohub.org/public/igem/BBa_I716101_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1974374
display id: annotation1974374
version: 1
top level: lcp
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974374/range1974374/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974374/range1974374/1
type: Range
persistent identity: range1974374
display id: range1974374
version: 1
direction: direction/reverse
top level: lcp
start: 1171
end: 1183
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1974379
display id: annotation1974379
version: 1
top level: lcp
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974379/range1974379/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974379/range1974379/1
type: Range
persistent identity: range1974379
display id: range1974379
version: 1
direction: direction/reverse
top level: lcp
start: 2918
end: 2922
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1974365
display id: annotation1974365
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974365/range1974365/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974365/range1974365/1
type: Range
persistent identity: range1974365
display id: range1974365
version: 1
direction: direction/reverse
top level: lcp
start: 1815
end: 1832
orientation: inline

promoter region
type: SequenceAnnotation
persistent identity: annotation1974373
display id: annotation1974373
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974373/range1974373/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974373/range1974373/1
type: Range
persistent identity: range1974373
display id: range1974373
version: 1
direction: direction/reverse
top level: lcp
start: 1188
end: 1207
orientation: inline

VF2 primer binding site
type: SequenceAnnotation
persistent identity: annotation1974380
display id: annotation1974380
version: 1
top level: lcp
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974380/range1974380/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974380/range1974380/1
type: Range
persistent identity: range1974380
display id: range1974380
version: 1
direction: direction/forward
top level: lcp
start: 3033
end: 3052
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1974367
display id: annotation1974367
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974367/range1974367/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974367/range1974367/1
type: Range
persistent identity: range1974367
display id: range1974367
version: 1
direction: direction/reverse
top level: lcp
start: 1318
end: 1337
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation1974360
display id: annotation1974360
version: 1
top level: lcp
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974360/range1974360/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974360/range1974360/1
type: Range
persistent identity: range1974360
display id: range1974360
version: 1
direction: direction/reverse
top level: lcp
start: 217
end: 1167
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1974372
display id: annotation1974372
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974372/range1974372/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974372/range1974372/1
type: Range
persistent identity: range1974372
display id: range1974372
version: 1
direction: direction/reverse
top level: lcp
start: 1198
end: 1203
orientation: inline

Berkeley suffix
type: SequenceAnnotation
persistent identity: annotation1974356
display id: annotation1974356
version: 1
top level: lcp
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974356/range1974356/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974356/range1974356/1
type: Range
persistent identity: range1974356
display id: range1974356
version: 1
direction: direction/forward
top level: lcp
start: 1
end: 15
orientation: inline

Berkeley prefix
type: SequenceAnnotation
persistent identity: annotation1974357
display id: annotation1974357
version: 1
top level: lcp
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974357/range1974357/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974357/range1974357/1
type: Range
persistent identity: range1974357
display id: range1974357
version: 1
direction: direction/forward
top level: lcp
start: 3151
end: 3165
orientation: inline

beta-lactamase promoter
type: SequenceAnnotation
persistent identity: annotation1974359
display id: annotation1974359
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974359/range1974359/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974359/range1974359/1
type: Range
persistent identity: range1974359
display id: range1974359
version: 1
direction: direction/reverse
top level: lcp
start: 2955
end: 2983
orientation: inline

beta-lactamase ampicillin resistance gene
type: SequenceAnnotation
persistent identity: annotation1974358
display id: annotation1974358
version: 1
top level: lcp
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974358/range1974358/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974358/range1974358/1
type: Range
persistent identity: range1974358
display id: range1974358
version: 1
direction: direction/reverse
top level: lcp
start: 2053
end: 2913
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1974362
display id: annotation1974362
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974362/range1974362/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974362/range1974362/1
type: Range
persistent identity: range1974362
display id: range1974362
version: 1
direction: direction/reverse
top level: lcp
start: 1901
end: 1914
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1974371
display id: annotation1974371
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974371/range1974371/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974371/range1974371/1
type: Range
persistent identity: range1974371
display id: range1974371
version: 1
direction: direction/reverse
top level: lcp
start: 1220
end: 1225
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1974364
display id: annotation1974364
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974364/range1974364/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974364/range1974364/1
type: Range
persistent identity: range1974364
display id: range1974364
version: 1
direction: direction/reverse
top level: lcp
start: 1838
end: 1861
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation1974366
display id: annotation1974366
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974366/range1974366/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974366/range1974366/1
type: Range
persistent identity: range1974366
display id: range1974366
version: 1
direction: direction/reverse
top level: lcp
start: 1336
end: 1418
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1974375
display id: annotation1974375
version: 1
top level: lcp
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974375/range1974375/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974375/range1974375/1
type: Range
persistent identity: range1974375
display id: range1974375
version: 1
direction: direction/reverse
top level: lcp
start: 1169
end: 1174
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1974368
display id: annotation1974368
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974368/range1974368/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974368/range1974368/1
type: Range
persistent identity: range1974368
display id: range1974368
version: 1
direction: direction/reverse
top level: lcp
start: 1297
end: 1316
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1974363
display id: annotation1974363
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974363/range1974363/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974363/range1974363/1
type: Range
persistent identity: range1974363
display id: range1974363
version: 1
direction: direction/reverse
top level: lcp
start: 1872
end: 1888
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1974369
display id: annotation1974369
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974369/range1974369/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974369/range1974369/1
type: Range
persistent identity: range1974369
display id: range1974369
version: 1
direction: direction/reverse
top level: lcp
start: 1265
end: 1284
orientation: inline

putative polyA
type: SequenceAnnotation
persistent identity: annotation1974376
display id: annotation1974376
version: 1
top level: lcp
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974376/range1974376/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974376/range1974376/1
type: Range
persistent identity: range1974376
display id: range1974376
version: 1
direction: direction/reverse
top level: lcp
start: 91
end: 106
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1974370
display id: annotation1974370
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974370/range1974370/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974370/range1974370/1
type: Range
persistent identity: range1974370
display id: range1974370
version: 1
direction: direction/reverse
top level: lcp
start: 1194
end: 1215
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation1974361
display id: annotation1974361
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974361/range1974361/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974361/range1974361/1
type: Range
persistent identity: range1974361
display id: range1974361
version: 1
direction: direction/reverse
top level: lcp
start: 1549
end: 1916
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1974377
display id: annotation1974377
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974377/range1974377/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974377/range1974377/1
type: Range
persistent identity: range1974377
display id: range1974377
version: 1
direction: direction/reverse
top level: lcp
start: 2957
end: 2962
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1974378
display id: annotation1974378
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974378/range1974378/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974378/range1974378/1
type: Range
persistent identity: range1974378
display id: range1974378
version: 1
direction: direction/reverse
top level: lcp
start: 2931
end: 2936
orientation: inline

BBa_K1351040
type: ComponentDefinition
persistent identity: BBa K1351040
display id: BBa_K1351040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBS0KPspac, an IPTG-inducible replicative expression vector for
creator: Jara Radeck
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1726_
m user id: 0
owner id: 6378
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pBS0KPspac, an IPTG-inducible replicative expression vector for ''Bacillus subtilis''.
mutable notes: Inducible expression vector with RFP-MCS downstream of Pspac.
mutable provenance: This vector is derived from pDG148, and the "repaired" version of pSBBs0K-Pspac.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1351040_sequence/1

BBa_K1480017
type: ComponentDefinition
persistent identity: BBa K1480017
display id: BBa_K1480017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB3C5 + MID and AF TALEN (K1480001) Target sites
creator: Roberto Alejandro Valderrama Alfaro
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1860_
m user id: 0
owner id: 19018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Backbone vector of pSB1C3 that contains two modifications with a TALEN target site on the center and after the origin of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains two TALEN target sites (MID+AF)
mutable notes: Modifications where inserted by PCR on both directions due to TALEN specificity.
mutable provenance: Derived from pSB3C5 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1480017_sequence/1

BBa_J72203
type: ComponentDefinition
persistent identity: BBa J72203
display id: BBa_J72203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kanamycin resistant split R6K vector
creator: Josh Kittleson
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:08:28Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: kanamycin resistant split R6K vector. Requires pir expression to replicate. referred to internally as pBjk2164
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72203_sequence/1

pSB8C15FRT
type: ComponentDefinition
persistent identity: BBa K864021
display id: BBa_K864021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864021_sequence/1

BBa_K106697
type: ComponentDefinition
persistent identity: BBa K106697
display id: BBa_K106697
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)
creator: Andrew Horwitz
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is a variant of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t), with the addition of 8X LexA operator sites directly downstream of the Adht1 terminator.
mutable notes: None.
mutable provenance: Variant of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: A overhang
sequence annotation: 8X LexA Operators
sequence annotation: ampicillin resistance marker
sequence annotation: pBR322 origin
sequence annotation: lac promoter
sequence annotation: Stop codons in all 3 frames
sequence annotation: T7 promoter
sequence annotation: D overhang
sequence annotation: LEU2 marker
sequence annotation: CEN ARS
sequence annotation: T3 promoter
sequence annotation: f1 origin
sequence annotation: Start codon
sequence annotation: Adh1 terminator
sequence annotation: lacZalpha reporter
sequence annotation: ampicillin resistance promoter
sequence annotation: Cyc1 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K106697_sequence/1

A overhang
type: SequenceAnnotation
persistent identity: annotation1999737
display id: annotation1999737
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999737/range1999737/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999737/range1999737/1
type: Range
persistent identity: range1999737
display id: range1999737
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6556
end: 6559
orientation: inline

8X LexA Operators
type: SequenceAnnotation
persistent identity: annotation1995079
display id: annotation1995079
version: 1
top level: BBa_K106697
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995079/range1995079/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995079/range1995079/1
type: Range
persistent identity: range1995079
display id: range1995079
version: 1
direction: direction/forward
top level: BBa_K106697
start: 210
end: 377
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1995018
display id: annotation1995018
version: 1
top level: BBa_K106697
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995018/range1995018/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995018/range1995018/1
type: Range
persistent identity: range1995018
display id: range1995018
version: 1
direction: direction/forward
top level: BBa_K106697
start: 4215
end: 5075
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999732
display id: annotation1999732
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999732/range1999732/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999732/range1999732/1
type: Range
persistent identity: range1999732
display id: range1999732
version: 1
direction: direction/forward
top level: BBa_K106697
start: 5230
end: 5849
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999733
display id: annotation1999733
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999733/range1999733/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999733/range1999733/1
type: Range
persistent identity: range1999733
display id: range1999733
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6158
end: 6187
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999735
display id: annotation1999735
version: 1
top level: BBa_K106697
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999735/range1999735/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999735/range1999735/1
type: Range
persistent identity: range1999735
display id: range1999735
version: 1
direction: direction/forward
top level: BBa_K106697
start: 11
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999728
display id: annotation1999728
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999728/range1999728/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999728/range1999728/1
type: Range
persistent identity: range1999728
display id: range1999728
version: 1
direction: direction/reverse
top level: BBa_K106697
start: 386
end: 404
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1995020
display id: annotation1995020
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995020/range1995020/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995020/range1995020/1
type: Range
persistent identity: range1995020
display id: range1995020
version: 1
direction: direction/forward
top level: BBa_K106697
start: 1
end: 4
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1995101
display id: annotation1995101
version: 1
top level: BBa_K106697
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995101/range1995101/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995101/range1995101/1
type: Range
persistent identity: range1995101
display id: range1995101
version: 1
direction: direction/forward
top level: BBa_K106697
start: 1732
end: 2838
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1995017
display id: annotation1995017
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995017/range1995017/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995017/range1995017/1
type: Range
persistent identity: range1995017
display id: range1995017
version: 1
direction: direction/reverse
top level: BBa_K106697
start: 3565
end: 4083
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999734
display id: annotation1999734
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999734/range1999734/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999734/range1999734/1
type: Range
persistent identity: range1999734
display id: range1999734
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6257
end: 6276
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999730
display id: annotation1999730
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999730/range1999730/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999730/range1999730/1
type: Range
persistent identity: range1999730
display id: range1999730
version: 1
direction: direction/forward
top level: BBa_K106697
start: 589
end: 895
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999736
display id: annotation1999736
version: 1
top level: BBa_K106697
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999736/range1999736/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999736/range1999736/1
type: Range
persistent identity: range1999736
display id: range1999736
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6547
end: 6549
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1995021
display id: annotation1995021
version: 1
top level: BBa_K106697
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995021/range1995021/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995021/range1995021/1
type: Range
persistent identity: range1995021
display id: range1995021
version: 1
direction: direction/forward
top level: BBa_K106697
start: 30
end: 209
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999729
display id: annotation1999729
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999729/range1999729/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999729/range1999729/1
type: Range
persistent identity: range1999729
display id: range1999729
version: 1
direction: direction/forward
top level: BBa_K106697
start: 408
end: 567
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999731
display id: annotation1999731
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999731/range1999731/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999731/range1999731/1
type: Range
persistent identity: range1999731
display id: range1999731
version: 1
direction: direction/forward
top level: BBa_K106697
start: 4145
end: 4173
orientation: inline

Cyc1 Promoter
type: SequenceAnnotation
persistent identity: annotation1995019
display id: annotation1995019
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995019/range1995019/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995019/range1995019/1
type: Range
persistent identity: range1995019
display id: range1995019
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6294
end: 6556
orientation: inline

BBa_J18968
type: ComponentDefinition
persistent identity: BBa J18968
display id: BBa_J18968
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSS3C1
creator: Sean Sleight
created: 2013-06-17T11:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2480
owning group id: 172
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Vector with pSS Prefix A and Suffix D, confers chloramphenicol resistance, p15a origin of replication, constitutively expresses luxR and araC.
mutable notes: Inserted luxR and araC to be constitutively expressed in the reverse direction on the vector. Removed restriction sites in prefix and suffix to improve assembly success rate.
mutable provenance: Modified pGA3K3 from 2011 University of Washington iGEM team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J18968_sequence/1

BBa_K1677201
type: ComponentDefinition
persistent identity: BBa K1677201
display id: BBa_K1677201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCPC
creator: Mackenzie Labine-Romain
created: 2015-08-20T11:00:00Z
modified: 2015-08-20T11:14:48Z
discontinued: false
dominant: false
group u list: _2095_
m user id: 25019
owner id: 25019
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This integrative plasmid facilitates transformation of Synechocystis PCC6803 and similar species. It inserts gene of choice into the Synechocystis cpc gene by homologous recombination.
mutable notes: Primers containing biobrick prefix and suffix as well and other described parts were used to make this backbone RFC10 compatible.
mutable provenance: Plasmid was purchased from open source plasmid repository Addgene.com (originally named cpc-PHLS (g)). It was modified to be biobrick compatible. Formighieri, C., & Melis, A. (2014). Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta, 240(2), 309-324.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

BBa_K1321300
type: ComponentDefinition
persistent identity: BBa K1321300
display id: BBa_K1321300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSEVA331-Bb - pSEVA331 with biobrick prefix and suffix
creator: Michael Florea
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1696_
m user id: 0
owner id: 20779
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is pSEVA331 converted into a biobrick compatible format by substituting the original multiple cloning site for biobrick prefix and suffix. pSEVA331 is a broad host range plasmid, capable of replication in E.coli and several other organisms. It is capable of replication in the cellulose producing bacterium Gluconacetobacter xylinum. We have used it as our standard backbone for G.xylinum genetic engineering.
mutable notes: -
mutable provenance: The original pSEVA331 backbone comes from the SEVA (Standard European Vector Architecture) collection.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: OriT
sequence annotation: Biobrick prefix
sequence annotation: Chloramphenicol acetyltransferase
sequence annotation: Biobrick suffix
sequence annotation: Rep
sequence: https://synbiohub.org/public/igem/BBa_K1321300_sequence/1

OriT
type: SequenceAnnotation
persistent identity: annotation2412975
display id: annotation2412975
version: 1
top level: BBa_K1321300
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321300/annotation2412975/range2412975/1

https://synbiohub.org/public/igem/BBa_K1321300/annotation2412975/range2412975/1
type: Range
persistent identity: range2412975
display id: range2412975
version: 1
direction: direction/forward
top level: BBa_K1321300
start: 954
end: 1189
orientation: inline

Biobrick prefix
type: SequenceAnnotation
persistent identity: annotation2408614
display id: annotation2408614
version: 1
top level: BBa_K1321300
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321300/annotation2408614/range2408614/1

https://synbiohub.org/public/igem/BBa_K1321300/annotation2408614/range2408614/1
type: Range
persistent identity: range2408614
display id: range2408614
version: 1
direction: direction/forward
top level: BBa_K1321300
start: 2872
end: 2893
orientation: inline

Chloramphenicol acetyltransferase
type: SequenceAnnotation
persistent identity: annotation2408619
display id: annotation2408619
version: 1
top level: BBa_K1321300
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1321300/annotation2408619/range2408619/1

https://synbiohub.org/public/igem/BBa_K1321300/annotation2408619/range2408619/1
type: Range
persistent identity: range2408619
display id: range2408619
version: 1
direction: direction/forward
top level: BBa_K1321300
start: 271
end: 930
orientation: inline

Biobrick suffix
type: SequenceAnnotation
persistent identity: annotation2408497
display id: annotation2408497
version: 1
top level: BBa_K1321300
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321300/annotation2408497/range2408497/1

https://synbiohub.org/public/igem/BBa_K1321300/annotation2408497/range2408497/1
type: Range
persistent identity: range2408497
display id: range2408497
version: 1
direction: direction/forward
top level: BBa_K1321300
start: 1
end: 22
orientation: inline

Rep
type: SequenceAnnotation
persistent identity: annotation2412990
display id: annotation2412990
version: 1
top level: BBa_K1321300
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321300/annotation2412990/range2412990/1

https://synbiohub.org/public/igem/BBa_K1321300/annotation2412990/range2412990/1
type: Range
persistent identity: range2412990
display id: range2412990
version: 1
direction: direction/reverse
top level: BBa_K1321300
start: 1198
end: 1860
orientation: inline

BBa_J99000
type: ComponentDefinition
persistent identity: BBa J99000
display id: BBa_J99000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSWK oriTRP4; [CmR]; oriVR6K; lambda_attP
creator: David Bikard
created: 2009-12-17T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _412_
m user id: 0
owner id: 1481
owning group id: 412
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This vector has the conditional origin of replication oriVR6K and can be maintained only in strains expressing the pir gene. It carries the origin of transfer of RP4 and can thus be used in conjugation assays in a donor cell expressing the RP4 conjugation machinery. It also carries the attP recombination site of the lambda phage in order to permit site specific integration at the attB site of E.coli chromosome mediated by the lambda integrase (Valens et al., 2004).
mutable notes: attP recombination site of the lambda phage was PCR amplified with oligos 5'-TGGAGCTCAAATCAAATAATGATTTTATTT-3' and 5'-GCTGGAGCTCCATGGTACGCGTGCTAGAGGCA-3', and cloned at the SacI site of pSW23T (Demarre et al., 2005). The multiple cloning site was then replaced with the BioBrick strandard restriction sites through inverse PCR with oligos 'GCTTCTAGAGTACTAGTAGCGGCCGCTGCAGGCGGTGGAGCTCAAATCAAATAATG' and 'AGTACTCTAGAAGCGGCCGCGAATTCTATCAAGCTTATCGATACCGTCGACG'
mutable provenance: This plasmid was constructed from pSW23T (Demarre et al., 2005)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: cat [CmR]
sequence annotation: oriVR6K
sequence annotation: lambda attP
sequence annotation: RP4oriT
sequence: https://synbiohub.org/public/igem/BBa_J99000_sequence/1

cat [CmR]
type: SequenceAnnotation
persistent identity: annotation2065221
display id: annotation2065221
version: 1
top level: BBa_J99000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J99000/annotation2065221/range2065221/1

https://synbiohub.org/public/igem/BBa_J99000/annotation2065221/range2065221/1
type: Range
persistent identity: range2065221
display id: range2065221
version: 1
direction: direction/reverse
top level: BBa_J99000
start: 1012
end: 1671
orientation: inline

oriVR6K
type: SequenceAnnotation
persistent identity: annotation2065220
display id: annotation2065220
version: 1
top level: BBa_J99000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J99000/annotation2065220/range2065220/1

https://synbiohub.org/public/igem/BBa_J99000/annotation2065220/range2065220/1
type: Range
persistent identity: range2065220
display id: range2065220
version: 1
direction: direction/forward
top level: BBa_J99000
start: 512
end: 938
orientation: inline

lambda attP
type: SequenceAnnotation
persistent identity: annotation2065219
display id: annotation2065219
version: 1
top level: BBa_J99000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J99000/annotation2065219/range2065219/1

https://synbiohub.org/public/igem/BBa_J99000/annotation2065219/range2065219/1
type: Range
persistent identity: range2065219
display id: range2065219
version: 1
direction: direction/forward
top level: BBa_J99000
start: 34
end: 450
orientation: inline

RP4oriT
type: SequenceAnnotation
persistent identity: annotation2065218
display id: annotation2065218
version: 1
top level: BBa_J99000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J99000/annotation2065218/range2065218/1

https://synbiohub.org/public/igem/BBa_J99000/annotation2065218/range2065218/1
type: Range
persistent identity: range2065218
display id: range2065218
version: 1
direction: direction/forward
top level: BBa_J99000
start: 1928
end: 2184
orientation: inline

BBa_K864013
type: ComponentDefinition
persistent identity: BBa K864013
display id: BBa_K864013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4C15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864013_sequence/1

BBa_K1480009
type: ComponentDefinition
persistent identity: BBa K1480009
display id: BBa_K1480009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3 + MID and BF TALEN (K1480001) Target sites
creator: Roberto Alejandro Valderrama Alfaro
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1860_
m user id: 0
owner id: 19018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Backbone vector of pSB1C3 that contains two modifications with a TALEN target site on the center and before the origin of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains two TALEN target sites (MID+BF)
mutable notes: Modifications where inserted by PCR on both directions due to TALEN specificity.
mutable provenance: Derived from pSB1C3 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1480009_sequence/1

BBa_K2077001
type: ComponentDefinition
persistent identity: BBa K2077001
display id: BBa_K2077001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB413-GPD: HIS3-selectable S. cerevisiae Expression Vector (RFC 10)
creator: Holly Cox
created: 2016-08-14T11:00:00Z
modified: 2016-08-16T01:10:45Z
discontinued: false
dominant: false
group u list: _2545_
m user id: 32279
owner id: 32279
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a RFC10 standard yeast expression vector adapted from the widely used plasmid pSB413-GPD. The MCS of p413-GPD was replaced with the standard BioBrick prefix and suffix and an illegal PstI restriction site near the HIS3 promoter of p413-GPD was removed. In the resultant plasmid, pSB413-GPD (5828 bp), the BioBrick prefix and suffix is situated between the strong constitutive yeast GPD promoter (TDH3) and CYC1 terminator allowing for facile cloning and expression of translational units in yeast. Standard VF2 and VR primer binding sites are included and M13 primer binding sites bracket the promoter and terminator. The plasmid has a ColE1 origin and ampicillin resistance for replication in E. coli. It also contains a yeast CEN/ARS and HIS3-selectable marker to provide high fidelity maintenance and low copy number in S. cerevisiae.
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: HIS3 Promoter
sequence annotation: BioBrick Prefix
sequence annotation: F1 ORI
sequence annotation: BioBrick Suffix
sequence annotation: ColEI ORI
sequence annotation: CYC1 Terminator
sequence annotation: GPD Promoter
sequence annotation: HIS3
sequence annotation: bla promoter
sequence annotation: Bla CDS
sequence annotation: CEN 6 Centromere
sequence: https://synbiohub.org/public/igem/BBa_K2077001_sequence/1

HIS3 Promoter
type: SequenceAnnotation
persistent identity: annotation2481477
display id: annotation2481477
version: 1
top level: BBa_K2077001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2077001/annotation2481477/range2481477/1

https://synbiohub.org/public/igem/BBa_K2077001/annotation2481477/range2481477/1
type: Range
persistent identity: range2481477
display id: range2481477
version: 1
direction: direction/reverse
top level: BBa_K2077001
start: 1853
end: 2039
orientation: inline

BioBrick Prefix
type: SequenceAnnotation
persistent identity: annotation2481483
display id: annotation2481483
version: 1
top level: BBa_K2077001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2077001/annotation2481483/range2481483/1

https://synbiohub.org/public/igem/BBa_K2077001/annotation2481483/range2481483/1
type: Range
persistent identity: range2481483
display id: range2481483
version: 1
direction: direction/forward
top level: BBa_K2077001
start: 5807
end: 5828
orientation: inline

F1 ORI
type: SequenceAnnotation
persistent identity: annotation2481475
display id: annotation2481475
version: 1
top level: BBa_K2077001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2077001/annotation2481475/range2481475/1

https://synbiohub.org/public/igem/BBa_K2077001/annotation2481475/range2481475/1
type: Range
persistent identity: range2481475
display id: range2481475
version: 1
direction: direction/forward
top level: BBa_K2077001
start: 478
end: 933
orientation: inline

BioBrick Suffix
type: SequenceAnnotation
persistent identity: annotation2481473
display id: annotation2481473
version: 1
top level: BBa_K2077001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2077001/annotation2481473/range2481473/1

https://synbiohub.org/public/igem/BBa_K2077001/annotation2481473/range2481473/1
type: Range
persistent identity: range2481473
display id: range2481473
version: 1
direction: direction/forward
top level: BBa_K2077001
start: 1
end: 21
orientation: inline

ColEI ORI
type: SequenceAnnotation
persistent identity: annotation2481481
display id: annotation2481481
version: 1
top level: BBa_K2077001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2077001/annotation2481481/range2481481/1

https://synbiohub.org/public/igem/BBa_K2077001/annotation2481481/range2481481/1
type: Range
persistent identity: range2481481
display id: range2481481
version: 1
direction: direction/forward
top level: BBa_K2077001
start: 4101
end: 4689
orientation: inline

CYC1 Terminator
type: SequenceAnnotation
persistent identity: annotation2481474
display id: annotation2481474
version: 1
top level: BBa_K2077001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2077001/annotation2481474/range2481474/1

https://synbiohub.org/public/igem/BBa_K2077001/annotation2481474/range2481474/1
type: Range
persistent identity: range2481474
display id: range2481474
version: 1
direction: direction/forward
top level: BBa_K2077001
start: 23
end: 270
orientation: inline

GPD Promoter
type: SequenceAnnotation
persistent identity: annotation2481482
display id: annotation2481482
version: 1
top level: BBa_K2077001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2077001/annotation2481482/range2481482/1

https://synbiohub.org/public/igem/BBa_K2077001/annotation2481482/range2481482/1
type: Range
persistent identity: range2481482
display id: range2481482
version: 1
direction: direction/forward
top level: BBa_K2077001
start: 5158
end: 5801
orientation: inline

HIS3
type: SequenceAnnotation
persistent identity: annotation2481476
display id: annotation2481476
version: 1
top level: BBa_K2077001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2077001/annotation2481476/range2481476/1

https://synbiohub.org/public/igem/BBa_K2077001/annotation2481476/range2481476/1
type: Range
persistent identity: range2481476
display id: range2481476
version: 1
direction: direction/reverse
top level: BBa_K2077001
start: 1193
end: 1852
orientation: inline

bla promoter
type: SequenceAnnotation
persistent identity: annotation2481479
display id: annotation2481479
version: 1
top level: BBa_K2077001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2077001/annotation2481479/range2481479/1

https://synbiohub.org/public/igem/BBa_K2077001/annotation2481479/range2481479/1
type: Range
persistent identity: range2481479
display id: range2481479
version: 1
direction: direction/forward
top level: BBa_K2077001
start: 2965
end: 3069
orientation: inline

Bla CDS
type: SequenceAnnotation
persistent identity: annotation2481480
display id: annotation2481480
version: 1
top level: BBa_K2077001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2077001/annotation2481480/range2481480/1

https://synbiohub.org/public/igem/BBa_K2077001/annotation2481480/range2481480/1
type: Range
persistent identity: range2481480
display id: range2481480
version: 1
direction: direction/forward
top level: BBa_K2077001
start: 3070
end: 3930
orientation: inline

CEN 6 Centromere
type: SequenceAnnotation
persistent identity: annotation2481478
display id: annotation2481478
version: 1
top level: BBa_K2077001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2077001/annotation2481478/range2481478/1

https://synbiohub.org/public/igem/BBa_K2077001/annotation2481478/range2481478/1
type: Range
persistent identity: range2481478
display id: range2481478
version: 1
direction: direction/forward
top level: BBa_K2077001
start: 2425
end: 2928
orientation: inline

V0200
type: ComponentDefinition
persistent identity: BBa J176121
display id: BBa_J176121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: V0200
creator: Brady Laughlin
created: 2011-10-17T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: FRT-marked mam. transfection vector (from Invitrogen pcDNA5 FRT/TO); no promoter; amp, hygro
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: SV40 early polyadenylation signal
sequence annotation: 3t3 2.0
sequence annotation: pUC Origin
sequence annotation: NotI SpeI
sequence annotation: Ampicillin resistance
sequence annotation: FRT
sequence annotation: Hygromycin resistance
sequence annotation: BGH
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_J176121_sequence/1

SV40 early polyadenylation signal
type: SequenceAnnotation
persistent identity: annotation2167347
display id: annotation2167347
version: 1
top level: V0200
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176121/annotation2167347/range2167347/1

https://synbiohub.org/public/igem/BBa_J176121/annotation2167347/range2167347/1
type: Range
persistent identity: range2167347
display id: range2167347
version: 1
direction: direction/forward
top level: V0200
start: 2150
end: 2280
orientation: inline

3t3 2.0
type: SequenceAnnotation
persistent identity: annotation2167341
display id: annotation2167341
version: 1
top level: V0200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176121/annotation2167341/range2167341/1

https://synbiohub.org/public/igem/BBa_J176121/annotation2167341/range2167341/1
type: Range
persistent identity: range2167341
display id: range2167341
version: 1
direction: direction/forward
top level: V0200
start: 205
end: 228
orientation: inline

pUC Origin
type: SequenceAnnotation
persistent identity: annotation2167348
display id: annotation2167348
version: 1
top level: V0200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176121/annotation2167348/range2167348/1

https://synbiohub.org/public/igem/BBa_J176121/annotation2167348/range2167348/1
type: Range
persistent identity: range2167348
display id: range2167348
version: 1
direction: direction/forward
top level: V0200
start: 2663
end: 3336
orientation: inline

NotI SpeI
type: SequenceAnnotation
persistent identity: annotation2167342
display id: annotation2167342
version: 1
top level: V0200
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J176121/annotation2167342/range2167342/1

https://synbiohub.org/public/igem/BBa_J176121/annotation2167342/range2167342/1
type: Range
persistent identity: range2167342
display id: range2167342
version: 1
direction: direction/forward
top level: V0200
start: 305
end: 335
orientation: inline

Ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation2167349
display id: annotation2167349
version: 1
top level: V0200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176121/annotation2167349/range2167349/1

https://synbiohub.org/public/igem/BBa_J176121/annotation2167349/range2167349/1
type: Range
persistent identity: range2167349
display id: range2167349
version: 1
direction: direction/reverse
top level: V0200
start: 3481
end: 4341
orientation: inline

FRT
type: SequenceAnnotation
persistent identity: annotation2167345
display id: annotation2167345
version: 1
top level: V0200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176121/annotation2167345/range2167345/1

https://synbiohub.org/public/igem/BBa_J176121/annotation2167345/range2167345/1
type: Range
persistent identity: range2167345
display id: range2167345
version: 1
direction: direction/forward
top level: V0200
start: 943
end: 980
orientation: inline

Hygromycin resistance
type: SequenceAnnotation
persistent identity: annotation2167346
display id: annotation2167346
version: 1
top level: V0200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176121/annotation2167346/range2167346/1

https://synbiohub.org/public/igem/BBa_J176121/annotation2167346/range2167346/1
type: Range
persistent identity: range2167346
display id: range2167346
version: 1
direction: direction/forward
top level: V0200
start: 998
end: 1990
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2167343
display id: annotation2167343
version: 1
top level: V0200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176121/annotation2167343/range2167343/1

https://synbiohub.org/public/igem/BBa_J176121/annotation2167343/range2167343/1
type: Range
persistent identity: range2167343
display id: range2167343
version: 1
direction: direction/reverse
top level: V0200
start: 430
end: 447
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2167350
display id: annotation2167350
version: 1
top level: V0200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176121/annotation2167350/range2167350/1

https://synbiohub.org/public/igem/BBa_J176121/annotation2167350/range2167350/1
type: Range
persistent identity: range2167350
display id: range2167350
version: 1
direction: direction/reverse
top level: V0200
start: 4342
end: 4440
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2167344
display id: annotation2167344
version: 1
top level: V0200
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176121/annotation2167344/range2167344/1

https://synbiohub.org/public/igem/BBa_J176121/annotation2167344/range2167344/1
type: Range
persistent identity: range2167344
display id: range2167344
version: 1
direction: direction/forward
top level: V0200
start: 436
end: 660
orientation: inline

pSBLB4C15
type: ComponentDefinition
persistent identity: BBa K1033210
display id: BBa_K1033210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. coli and lactobacilli shuttle vector with chloramphenicol resistance
creator: Anders Edlund
created: 2013-08-21T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1340_
m user id: 0
owner id: 14332
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: E. coli Lactobacilli shuttle vector pSBLb4C15-red pSBLb4C15 is a shuttle vector containing the ori pSH71 originating from the plasmid pJP059 which was taken from Lactococcus lactis.
mutable notes: Coming Test
mutable provenance: Lactococcus lactis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Help:Barcodes
sequence annotation: BBa_E1010
sequence annotation: mrfp1
sequence: https://synbiohub.org/public/igem/BBa_K1033210_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2332020
display id: annotation2332020
version: 1
top level: pSBLB4C15
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1033210/annotation2332020/range2332020/1

https://synbiohub.org/public/igem/BBa_K1033210/annotation2332020/range2332020/1
type: Range
persistent identity: range2332020
display id: range2332020
version: 1
direction: direction/forward
top level: pSBLB4C15
start: 682
end: 706
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2332021
display id: annotation2332021
version: 1
top level: pSBLB4C15
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1033210/annotation2332021/range2332021/1

https://synbiohub.org/public/igem/BBa_K1033210/annotation2332021/range2332021/1
type: Range
persistent identity: range2332021
display id: range2332021
version: 1
direction: direction/reverse
top level: pSBLB4C15
start: 1
end: 706
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation2332019
display id: annotation2332019
version: 1
top level: pSBLB4C15
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033210/annotation2332019/range2332019/1

https://synbiohub.org/public/igem/BBa_K1033210/annotation2332019/range2332019/1
type: Range
persistent identity: range2332019
display id: range2332019
version: 1
direction: direction/forward
top level: pSBLB4C15
start: 1
end: 675
orientation: inline

BBa_K864012
type: ComponentDefinition
persistent identity: BBa K864012
display id: BBa_K864012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864012_sequence/1

BBa_K2066523
type: ComponentDefinition
persistent identity: BBa K2066523
display id: BBa_K2066523
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F30/2xBroccoli
creator: John Mitchell
created: 2016-10-08T11:00:00Z
modified: 2016-10-09T09:22:33Z
discontinued: false
dominant: false
group u list: _2534_
m user id: 31547
owner id: 31547
owning group id: 9
status: status/Planning
bookmark: false
mutable description: for use in K2066050
mutable notes: N/A
mutable provenance: from the addGene plasmid https://www.addgene.org/66843/
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K2066523_sequence/1

BBa_J119049
type: ComponentDefinition
persistent identity: BBa J119049
display id: BBa_J119049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4C8
creator: A. Malcolm Campbell and Todd Eckdahl
created: 2012-01-23T12:00:00Z
modified: 2015-08-31T04:08:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Insulated low copy vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. Uses I50042 low copy pSC101 replication origin.
mutable notes: Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.
mutable provenance: de novo DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: stuffer
sequence annotation: VF2 primer
sequence annotation: Chloramphenicol resistance gene
sequence annotation: Prefix
sequence annotation: I50042 low copy pSC101 origin
sequence annotation: B0025 terminator
sequence annotation: VR primer
sequence annotation: B0010 terminator
sequence annotation: Suffix
sequence: https://synbiohub.org/public/igem/BBa_J119049_sequence/1

stuffer
type: SequenceAnnotation
persistent identity: annotation2169716
display id: annotation2169716
version: 1
top level: BBa_J119049
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119049/annotation2169716/range2169716/1

https://synbiohub.org/public/igem/BBa_J119049/annotation2169716/range2169716/1
type: Range
persistent identity: range2169716
display id: range2169716
version: 1
direction: direction/forward
top level: BBa_J119049
start: 3566
end: 3577
orientation: inline

VF2 primer
type: SequenceAnnotation
persistent identity: annotation2169717
display id: annotation2169717
version: 1
top level: BBa_J119049
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119049/annotation2169717/range2169717/1

https://synbiohub.org/public/igem/BBa_J119049/annotation2169717/range2169717/1
type: Range
persistent identity: range2169717
display id: range2169717
version: 1
direction: direction/forward
top level: BBa_J119049
start: 3406
end: 3425
orientation: inline

Chloramphenicol resistance gene
type: SequenceAnnotation
persistent identity: annotation2169722
display id: annotation2169722
version: 1
top level: BBa_J119049
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J119049/annotation2169722/range2169722/1

https://synbiohub.org/public/igem/BBa_J119049/annotation2169722/range2169722/1
type: Range
persistent identity: range2169722
display id: range2169722
version: 1
direction: direction/reverse
top level: BBa_J119049
start: 2633
end: 3294
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2169714
display id: annotation2169714
version: 1
top level: BBa_J119049
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119049/annotation2169714/range2169714/1

https://synbiohub.org/public/igem/BBa_J119049/annotation2169714/range2169714/1
type: Range
persistent identity: range2169714
display id: range2169714
version: 1
direction: direction/forward
top level: BBa_J119049
start: 3544
end: 3565
orientation: inline

I50042 low copy pSC101 origin
type: SequenceAnnotation
persistent identity: annotation2169721
display id: annotation2169721
version: 1
top level: BBa_J119049
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119049/annotation2169721/range2169721/1

https://synbiohub.org/public/igem/BBa_J119049/annotation2169721/range2169721/1
type: Range
persistent identity: range2169721
display id: range2169721
version: 1
direction: direction/forward
top level: BBa_J119049
start: 314
end: 2350
orientation: inline

B0025 terminator
type: SequenceAnnotation
persistent identity: annotation2169719
display id: annotation2169719
version: 1
top level: BBa_J119049
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J119049/annotation2169719/range2169719/1

https://synbiohub.org/public/igem/BBa_J119049/annotation2169719/range2169719/1
type: Range
persistent identity: range2169719
display id: range2169719
version: 1
direction: direction/forward
top level: BBa_J119049
start: 22
end: 205
orientation: inline

VR primer
type: SequenceAnnotation
persistent identity: annotation2169718
display id: annotation2169718
version: 1
top level: BBa_J119049
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J119049/annotation2169718/range2169718/1

https://synbiohub.org/public/igem/BBa_J119049/annotation2169718/range2169718/1
type: Range
persistent identity: range2169718
display id: range2169718
version: 1
direction: direction/reverse
top level: BBa_J119049
start: 208
end: 227
orientation: inline

B0010 terminator
type: SequenceAnnotation
persistent identity: annotation2169720
display id: annotation2169720
version: 1
top level: BBa_J119049
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J119049/annotation2169720/range2169720/1

https://synbiohub.org/public/igem/BBa_J119049/annotation2169720/range2169720/1
type: Range
persistent identity: range2169720
display id: range2169720
version: 1
direction: direction/forward
top level: BBa_J119049
start: 3504
end: 3543
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2169715
display id: annotation2169715
version: 1
top level: BBa_J119049
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119049/annotation2169715/range2169715/1

https://synbiohub.org/public/igem/BBa_J119049/annotation2169715/range2169715/1
type: Range
persistent identity: range2169715
display id: range2169715
version: 1
direction: direction/forward
top level: BBa_J119049
start: 1
end: 21
orientation: inline

BBa_K1185003
type: ComponentDefinition
persistent identity: BBa K1185003
display id: BBa_K1185003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Integration vector for Bacillus subtilis derived from pSac-Cm
creator: Vincent Leonardo
created: 2013-10-03T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1498_
m user id: 0
owner id: 16794
owning group id: 9
sample status: No part sequence
status: status/Deleted
bookmark: false
mutable description: This plasmid was derived from pSac-Cm by insertion of biobrick compatible restriction sites (prefixes and suffixes), a terminator (BBa_B0015) after the suffixes sequences, and red fluorescent protein sequence (RFP) in between the prefix and suffix in its multiple cloning sites (MCS). New biobricks can be inserted into this vector by replacement of the RFP biobrick, and selection of the white colonies. This backbone has a multi host replication origin and replicates in E. coli. The plasmid is designed to integrate a cloned insert into the B. subtilis chromosome via double recombination between plasmid and chromosomal sacA sequences, and in order to check for integration a simple Phenol Red Sucrose test can be perform, if sacA is no longer functional, they will remain ph7, if they are still able to digest sucrose their pH will be under 5.
mutable notes: We found that that the integration plasmid that groningen send to the registry were unable to integrate in B. subtilis. This is due to the high number of mutations in the sacA integration region.
mutable provenance: Groningen iGEM 2012 team
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

BBa_K351011
type: ComponentDefinition
persistent identity: BBa K351011
display id: BBa_K351011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGEM-easy-T vector
creator: NAMBIN YIM
created: 2010-11-01T12:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
group u list: _462_
m user id: 0
owner id: 6038
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pGEM-T-easy Vector is linearized vector with a single 3??-terminal thymidine at both ends. The T-overhangs at the insertion site greatly improve the efficiency of ligation of PCR products by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases. pGEM-T-easy Vector is high-copy-number vector containing T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of the enzyme β-galactosidase. Insertional inactivation of the α-peptide allows identification of recombinants by blue/white screening on indicator plates. Choice of Restriction Sites for Release of Insert: pGEM-T Easy Vector contains numerous restriction sites within the multiple cloning region. Its multiple cloning region is flanked by recognition sites for the restriction enzymes EcoRI, BstZI and NotI, providing three single-enzyme digestions for release of the insert. Alternatively, a double-digestion may be used to release the insert from either vector. pGEM-T Easy Vector System is supplied with 2X Rapid Ligation Buffer. Ligation reactions using this buffer may be incubated for 1 hour at room temperature. The incubation period may be extended to increase the number of colonies after transformation. Generally, an overnight incubation at 4??C produces the maximum number of transformants.
mutable notes: This gene was designed by Promega. We take no credit. It has been sponsored by Bioneer.
mutable provenance: Synthesized gene by Promega.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: multiple cloning region
sequence annotation: lac operon sequences
sequence annotation: pUC/M13 Forward Sequencing Primer binding site
sequence annotation: T7 RNA polymerase transcription initiation site
sequence annotation: BBa_K351011
sequence annotation: T7 RNA polymerase promoter (-17 to +3)
sequence annotation: phage f1 region
sequence annotation: lacZ start codon
sequence annotation: pUC/M13 Reverse Sequencing Primer binding site
sequence annotation: SP6 RNA polymerase transcription initiation site
sequence annotation: lac operator
sequence annotation: beta-lactamase coding region
sequence annotation: SP6 RNA polymerase promoter (-17 to +3)
sequence: https://synbiohub.org/public/igem/BBa_K351011_sequence/1

multiple cloning region
type: SequenceAnnotation
persistent identity: annotation2113712
display id: annotation2113712
version: 1
top level: BBa_K351011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113712/range2113712/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113712/range2113712/1
type: Range
persistent identity: range2113712
display id: range2113712
version: 1
direction: direction/forward
top level: BBa_K351011
start: 10
end: 128
orientation: inline

lac operon sequences
type: SequenceAnnotation
persistent identity: annotation2113721
display id: annotation2113721
version: 1
top level: BBa_K351011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113721/range2113721/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113721/range2113721/1
type: Range
persistent identity: range2113721
display id: range2113721
version: 1
direction: direction/forward
top level: BBa_K351011
start: 2836
end: 2996
orientation: inline

pUC/M13 Forward Sequencing Primer binding site
type: SequenceAnnotation
persistent identity: annotation2113722
display id: annotation2113722
version: 1
top level: BBa_K351011
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113722/range2113722/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113722/range2113722/1
type: Range
persistent identity: range2113722
display id: range2113722
version: 1
direction: direction/forward
top level: BBa_K351011
start: 2949
end: 2972
orientation: inline

T7 RNA polymerase transcription initiation site
type: SequenceAnnotation
persistent identity: annotation2113710
display id: annotation2113710
version: 1
top level: BBa_K351011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113710/range2113710/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113710/range2113710/1
type: Range
persistent identity: range2113710
display id: range2113710
version: 1
direction: direction/forward
top level: BBa_K351011
start: 1
end: 1
orientation: inline

BBa_K351011
type: SequenceAnnotation
persistent identity: annotation2113709
display id: annotation2113709
version: 1
top level: BBa_K351011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113709/range2113709/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113709/range2113709/1
type: Range
persistent identity: range2113709
display id: range2113709
version: 1
direction: direction/reverse
top level: BBa_K351011
start: 1
end: 3015
orientation: inline

T7 RNA polymerase promoter (-17 to +3)
type: SequenceAnnotation
persistent identity: annotation2113723
display id: annotation2113723
version: 1
top level: BBa_K351011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113723/range2113723/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113723/range2113723/1
type: Range
persistent identity: range2113723
display id: range2113723
version: 1
direction: direction/forward
top level: BBa_K351011
start: 2999
end: 3015
orientation: inline

lac operon sequences
type: SequenceAnnotation
persistent identity: annotation2113715
display id: annotation2113715
version: 1
top level: BBa_K351011
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113715/range2113715/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113715/range2113715/1
type: Range
persistent identity: range2113715
display id: range2113715
version: 1
direction: direction/forward
top level: BBa_K351011
start: 166
end: 395
orientation: inline

phage f1 region
type: SequenceAnnotation
persistent identity: annotation2113720
display id: annotation2113720
version: 1
top level: BBa_K351011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113720/range2113720/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113720/range2113720/1
type: Range
persistent identity: range2113720
display id: range2113720
version: 1
direction: direction/forward
top level: BBa_K351011
start: 2380
end: 2835
orientation: inline

T7 RNA polymerase promoter (-17 to +3)
type: SequenceAnnotation
persistent identity: annotation2113711
display id: annotation2113711
version: 1
top level: BBa_K351011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113711/range2113711/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113711/range2113711/1
type: Range
persistent identity: range2113711
display id: range2113711
version: 1
direction: direction/forward
top level: BBa_K351011
start: 1
end: 3
orientation: inline

lacZ start codon
type: SequenceAnnotation
persistent identity: annotation2113717
display id: annotation2113717
version: 1
top level: BBa_K351011
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113717/range2113717/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113717/range2113717/1
type: Range
persistent identity: range2113717
display id: range2113717
version: 1
direction: direction/forward
top level: BBa_K351011
start: 180
end: 180
orientation: inline

pUC/M13 Reverse Sequencing Primer binding site
type: SequenceAnnotation
persistent identity: annotation2113716
display id: annotation2113716
version: 1
top level: BBa_K351011
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113716/range2113716/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113716/range2113716/1
type: Range
persistent identity: range2113716
display id: range2113716
version: 1
direction: direction/forward
top level: BBa_K351011
start: 176
end: 197
orientation: inline

SP6 RNA polymerase transcription initiation site
type: SequenceAnnotation
persistent identity: annotation2113714
display id: annotation2113714
version: 1
top level: BBa_K351011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113714/range2113714/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113714/range2113714/1
type: Range
persistent identity: range2113714
display id: range2113714
version: 1
direction: direction/forward
top level: BBa_K351011
start: 141
end: 141
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2113718
display id: annotation2113718
version: 1
top level: BBa_K351011
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113718/range2113718/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113718/range2113718/1
type: Range
persistent identity: range2113718
display id: range2113718
version: 1
direction: direction/forward
top level: BBa_K351011
start: 200
end: 216
orientation: inline

beta-lactamase coding region
type: SequenceAnnotation
persistent identity: annotation2113719
display id: annotation2113719
version: 1
top level: BBa_K351011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113719/range2113719/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113719/range2113719/1
type: Range
persistent identity: range2113719
display id: range2113719
version: 1
direction: direction/forward
top level: BBa_K351011
start: 1337
end: 2197
orientation: inline

SP6 RNA polymerase promoter (-17 to +3)
type: SequenceAnnotation
persistent identity: annotation2113713
display id: annotation2113713
version: 1
top level: BBa_K351011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K351011/annotation2113713/range2113713/1

https://synbiohub.org/public/igem/BBa_K351011/annotation2113713/range2113713/1
type: Range
persistent identity: range2113713
display id: range2113713
version: 1
direction: direction/forward
top level: BBa_K351011
start: 139
end: 158
orientation: inline

pSB3C5
type: ComponentDefinition
persistent identity: pSB3C5
display id: pSB3C5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3C5 is a low to medium copy plasmid based with chloramphenicol resistance. It has a p15A-derived replication origin. pSB3C5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the chloramphenicol resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00102
component: BBa_B0042
component: BBa_G0002
component: BBa_G0004
component: BBa_B0044
component: BBa_G0003
component: BBa_G00001
component: BBa_G00100
component: BBa_B0062
component: BBa_P1004
component: BBa_B0054
component: BBa_B0055
component: BBa_B0043
component: BBa_B0053
component: BBa_G00000
component: BBa_I50032
sequence annotation: BBa_B0055
sequence annotation: BBa_B0053
sequence annotation: BBa_B0062
sequence annotation: BBa_G00001
sequence annotation: BBa_G00100
sequence annotation: BBa_I50032
sequence annotation: BBa_B0043
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_G00102
sequence annotation: BBa_G0003
sequence annotation: BBa_G0002
sequence annotation: BBa_P1004
sequence annotation: BBa_G00000
sequence annotation: BBa_G0004
sequence: https://synbiohub.org/public/igem/pSB3C5_sequence/1

BBa_G00102
type: Component
persistent identity: component1928326
display id: component1928326
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component1928322
display id: component1928322
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0002
type: Component
persistent identity: component1928333
display id: component1928333
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0004
type: Component
persistent identity: component1928336
display id: component1928336
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0044
type: Component
persistent identity: component1928315
display id: component1928315
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G0003
type: Component
persistent identity: component1928329
display id: component1928329
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00001
type: Component
persistent identity: component1928310
display id: component1928310
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00100
type: Component
persistent identity: component1928339
display id: component1928339
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0062
type: Component
persistent identity: component1928328
display id: component1928328
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_P1004
type: Component
persistent identity: component1928335
display id: component1928335
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0042
type: Component
persistent identity: component1928349
display id: component1928349
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0054
type: Component
persistent identity: component1928325
display id: component1928325
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0055
type: Component
persistent identity: component1928342
display id: component1928342
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0043
type: Component
persistent identity: component1928354
display id: component1928354
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0053
type: Component
persistent identity: component1928338
display id: component1928338
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G00000
type: Component
persistent identity: component1928356
display id: component1928356
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_I50032
type: Component
persistent identity: component1928332
display id: component1928332
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1928342
display id: annotation1928342
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928342/range1928342/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3C5/annotation1928342/range1928342/1
type: Range
persistent identity: range1928342
display id: range1928342
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2614
end: 2691
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1928338
display id: annotation1928338
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928338/range1928338/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3C5/annotation1928338/range1928338/1
type: Range
persistent identity: range1928338
display id: range1928338
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2522
end: 2593
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1928328
display id: annotation1928328
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928328/range1928328/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3C5/annotation1928328/range1928328/1
type: Range
persistent identity: range1928328
display id: range1928328
version: 1
direction: direction/reverse
top level: pSB3C5
start: 136
end: 176
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1928310
display id: annotation1928310
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928310/range1928310/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3C5/annotation1928310/range1928310/1
type: Range
persistent identity: range1928310
display id: range1928310
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1
end: 21
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1928339
display id: annotation1928339
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928339/range1928339/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3C5/annotation1928339/range1928339/1
type: Range
persistent identity: range1928339
display id: range1928339
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2594
end: 2613
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1928332
display id: annotation1928332
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928332/range1928332/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3C5/annotation1928332/range1928332/1
type: Range
persistent identity: range1928332
display id: range1928332
version: 1
direction: direction/reverse
top level: pSB3C5
start: 184
end: 1737
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1928354
display id: annotation1928354
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928354/range1928354/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3C5/annotation1928354/range1928354/1
type: Range
persistent identity: range1928354
display id: range1928354
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2704
end: 2716
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1928315
display id: annotation1928315
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928315/range1928315/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3C5/annotation1928315/range1928315/1
type: Range
persistent identity: range1928315
display id: range1928315
version: 1
direction: direction/reverse
top level: pSB3C5
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928349
display id: annotation1928349
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928349/range1928349/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3C5/annotation1928349/range1928349/1
type: Range
persistent identity: range1928349
display id: range1928349
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2692
end: 2703
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1928325
display id: annotation1928325
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928325/range1928325/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3C5/annotation1928325/range1928325/1
type: Range
persistent identity: range1928325
display id: range1928325
version: 1
direction: direction/reverse
top level: pSB3C5
start: 47
end: 115
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1928326
display id: annotation1928326
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928326/range1928326/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3C5/annotation1928326/range1928326/1
type: Range
persistent identity: range1928326
display id: range1928326
version: 1
direction: direction/reverse
top level: pSB3C5
start: 116
end: 135
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1928329
display id: annotation1928329
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928329/range1928329/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3C5/annotation1928329/range1928329/1
type: Range
persistent identity: range1928329
display id: range1928329
version: 1
direction: direction/reverse
top level: pSB3C5
start: 177
end: 183
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1928333
display id: annotation1928333
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928333/range1928333/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3C5/annotation1928333/range1928333/1
type: Range
persistent identity: range1928333
display id: range1928333
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1738
end: 1745
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928322
display id: annotation1928322
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928322/range1928322/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3C5/annotation1928322/range1928322/1
type: Range
persistent identity: range1928322
display id: range1928322
version: 1
direction: direction/reverse
top level: pSB3C5
start: 35
end: 46
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation1928335
display id: annotation1928335
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928335/range1928335/1
component: BBa_P1004

https://synbiohub.org/public/igem/pSB3C5/annotation1928335/range1928335/1
type: Range
persistent identity: range1928335
display id: range1928335
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1746
end: 2514
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1928356
display id: annotation1928356
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928356/range1928356/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3C5/annotation1928356/range1928356/1
type: Range
persistent identity: range1928356
display id: range1928356
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2717
end: 2738
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1928336
display id: annotation1928336
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928336/range1928336/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3C5/annotation1928336/range1928336/1
type: Range
persistent identity: range1928336
display id: range1928336
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2515
end: 2521
orientation: inline

BBa_K777133
type: ComponentDefinition
persistent identity: BBa K777133
display id: BBa_K777133
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Similar to pSB1C3, with RFP reporter
creator: Team Goettingen
created: 2012-09-27T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to [http://partsregistry.org/Part:BBa_J61002 J61002] but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.
mutable notes: * No specific design considerations.
mutable provenance: * This part is a compination of E1010, B0034 and pSB1C3 from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rep (pMB1)
sequence annotation: ORI
sequence annotation: PstI
sequence annotation: E1010
sequence annotation: XbaI
sequence annotation: BB0034
sequence annotation: EcoRI
sequence annotation: mRFP1
sequence annotation: SpeI
sequence annotation: VR primer binding site
sequence annotation: Chloramphenicol resistance
sequence annotation: RBS
sequence annotation: VF2 primer binding site
sequence: https://synbiohub.org/public/igem/BBa_K777133_sequence/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2203655
display id: annotation2203655
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203655/range2203655/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203655/range2203655/1
type: Range
persistent identity: range2203655
display id: range2203655
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1129
end: 1735
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2203662
display id: annotation2203662
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203662/range2203662/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203662/range2203662/1
type: Range
persistent identity: range2203662
display id: range2203662
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1144
end: 1144
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2203666
display id: annotation2203666
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203666/range2203666/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203666/range2203666/1
type: Range
persistent identity: range2203666
display id: range2203666
version: 1
direction: direction/forward
top level: BBa_K777133
start: 884
end: 889
orientation: inline

E1010
type: SequenceAnnotation
persistent identity: annotation2203654
display id: annotation2203654
version: 1
top level: BBa_K777133
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203654/range2203654/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203654/range2203654/1
type: Range
persistent identity: range2203654
display id: range2203654
version: 1
direction: direction/forward
top level: BBa_K777133
start: 26
end: 703
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2203668
display id: annotation2203668
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203668/range2203668/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203668/range2203668/1
type: Range
persistent identity: range2203668
display id: range2203668
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2932
end: 2937
orientation: inline

BB0034
type: SequenceAnnotation
persistent identity: annotation2203652
display id: annotation2203652
version: 1
top level: BBa_K777133
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203652/range2203652/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203652/range2203652/1
type: Range
persistent identity: range2203652
display id: range2203652
version: 1
direction: direction/forward
top level: BBa_K777133
start: 8
end: 19
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2203667
display id: annotation2203667
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203667/range2203667/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203667/range2203667/1
type: Range
persistent identity: range2203667
display id: range2203667
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2917
end: 2922
orientation: inline

mRFP1
type: SequenceAnnotation
persistent identity: annotation2203653
display id: annotation2203653
version: 1
top level: BBa_K777133
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203653/range2203653/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203653/range2203653/1
type: Range
persistent identity: range2203653
display id: range2203653
version: 1
direction: direction/forward
top level: BBa_K777133
start: 26
end: 703
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2203665
display id: annotation2203665
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203665/range2203665/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203665/range2203665/1
type: Range
persistent identity: range2203665
display id: range2203665
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1
end: 6
orientation: inline

VR primer binding site
type: SequenceAnnotation
persistent identity: annotation2203663
display id: annotation2203663
version: 1
top level: BBa_K777133
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203663/range2203663/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203663/range2203663/1
type: Range
persistent identity: range2203663
display id: range2203663
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1023
end: 1042
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2203657
display id: annotation2203657
version: 1
top level: BBa_K777133
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203657/range2203657/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203657/range2203657/1
type: Range
persistent identity: range2203657
display id: range2203657
version: 1
direction: direction/reverse
top level: BBa_K777133
start: 2028
end: 2687
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203651
display id: annotation2203651
version: 1
top level: BBa_K777133
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203651/range2203651/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203651/range2203651/1
type: Range
persistent identity: range2203651
display id: range2203651
version: 1
direction: direction/forward
top level: BBa_K777133
start: 8
end: 19
orientation: inline

VF2 primer binding site
type: SequenceAnnotation
persistent identity: annotation2203664
display id: annotation2203664
version: 1
top level: BBa_K777133
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203664/range2203664/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203664/range2203664/1
type: Range
persistent identity: range2203664
display id: range2203664
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2799
end: 2818
orientation: inline

pSB2K0
type: ComponentDefinition
persistent identity: pSB2K0
display id: pSB2K0
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB2K0 (pSCANS-BNL; Replaced by pSB2K3)
creator: Brookhaven National Lab
created: 2005-06-12T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_1_
m user id: 0
owner id: 25
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Warning, this plasmid does not have a proper BioBrick cloning site.
mutable notes: "A new plasmid vector, pSCANS, has been constructed which allows rapid generation of an ordered set of nested deletions from either strand of a cloned DNA fragment. pSCANS is based on the low-copy F replicon. The size of the vector DNA has been reduced to the 4.4-kbp range by removing the 2.5-kbp sop (stability of plasmid genes) region. The resulting plasmid has the low copy number typical of F plasmids and it remains stable enough to be easily maintained by growth in the presence of kanamycin, the selective antibiotic. DNA in amounts convenient for sequencing is readily obtained by amplification from an IPTG-inducible P1 lytic replicon. The vector's multiple cloning region (MCR), which has several unique sites for both shotgun and directional cloning, is flanked on one side by recognition sequences for the extremely rare cutting intron encoded nucleases I-CeuI and I-SceI, and on the other side by a recognition sequence for another intron encoded enzyme, PI-PspI and a nicking site for the phage f1 protein, gpII, that initiates f1 rolling circle DNA replication. Cleavage with the intron encoded enzymes leaves four-base 3' overhangs that are resistant to digestion with E. coli ExoIII. Between these sites and the MCR are recognition sites for several rare 8-base cutters that leave ExoIII sensitive termini. Double cutting with one intron encoded enzyme and an adjacent rare cutting restriction endonuclease allows for unidirectional 3' to 5' digestion across the insert with ExoIII. Alternatively, plasmid linearized with I-SceI can be blunt ended to produce an ExoIII sensitive end an ent generates a good distribution of deletion clones following electroporation. Deletion clones are sized and sequenced using vector specific forward and reverse primers. "
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: kanR
sequence: https://synbiohub.org/public/igem/pSB2K0_sequence/1

kanR
type: SequenceAnnotation
persistent identity: annotation1782703
display id: annotation1782703
version: 1
top level: pSB2K0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K0/annotation1782703/range1782703/1

https://synbiohub.org/public/igem/pSB2K0/annotation1782703/range1782703/1
type: Range
persistent identity: range1782703
display id: range1782703
version: 1
direction: direction/forward
top level: pSB2K0
start: 1
end: 816
orientation: inline

BBa_K106698
type: ComponentDefinition
persistent identity: BBa K106698
display id: BBa_K106698
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, 8X LexAOps Fig1P, Adh1t)
creator: Andrew Horwitz
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Acceptor for AarI AD parts, with 8X LexA operators directly upstream of the medium strong Cyc1 promoter.
mutable notes: LexA Ops were cloned non-directionally into the PspOMI site. Inner site is broken, thus the cassette can be subcloned from this plasmid by PspOMI/SacI digestion (for example, to change markers).
mutable provenance: Modification of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: lac promoter
sequence annotation: A overhang
sequence annotation: ampicillin marker promoter
sequence annotation: Stop codons in all 3 frames
sequence annotation: 8X LexA Operators
sequence annotation: LEU2 marker
sequence annotation: lacZalpha reporter
sequence annotation: T3 promoter
sequence annotation: D overhang
sequence annotation: pBR322 origin
sequence annotation: f1 origin
sequence annotation: ampicillin resistance marker
sequence annotation: CEN ARS
sequence annotation: Adh1 terminator
sequence annotation: Fig1 Promoter
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K106698_sequence/1

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999743
display id: annotation1999743
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999743/range1999743/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999743/range1999743/1
type: Range
persistent identity: range1999743
display id: range1999743
version: 1
direction: direction/forward
top level: BBa_K106698
start: 5996
end: 6025
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999747
display id: annotation1999747
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999747/range1999747/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999747/range1999747/1
type: Range
persistent identity: range1999747
display id: range1999747
version: 1
direction: direction/forward
top level: BBa_K106698
start: 7309
end: 7312
orientation: inline

ampicillin marker promoter
type: SequenceAnnotation
persistent identity: annotation1999741
display id: annotation1999741
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999741/range1999741/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999741/range1999741/1
type: Range
persistent identity: range1999741
display id: range1999741
version: 1
direction: direction/forward
top level: BBa_K106698
start: 2983
end: 4011
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999746
display id: annotation1999746
version: 1
top level: BBa_K106698
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999746/range1999746/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999746/range1999746/1
type: Range
persistent identity: range1999746
display id: range1999746
version: 1
direction: direction/forward
top level: BBa_K106698
start: 11
end: 21
orientation: inline

8X LexA Operators
type: SequenceAnnotation
persistent identity: annotation1995345
display id: annotation1995345
version: 1
top level: BBa_K106698
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995345/range1995345/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995345/range1995345/1
type: Range
persistent identity: range1995345
display id: range1995345
version: 1
direction: direction/forward
top level: BBa_K106698
start: 6132
end: 6299
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1995348
display id: annotation1995348
version: 1
top level: BBa_K106698
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995348/range1995348/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995348/range1995348/1
type: Range
persistent identity: range1995348
display id: range1995348
version: 1
direction: direction/forward
top level: BBa_K106698
start: 1570
end: 2676
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999739
display id: annotation1999739
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999739/range1999739/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999739/range1999739/1
type: Range
persistent identity: range1999739
display id: range1999739
version: 1
direction: direction/forward
top level: BBa_K106698
start: 246
end: 405
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999744
display id: annotation1999744
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999744/range1999744/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999744/range1999744/1
type: Range
persistent identity: range1999744
display id: range1999744
version: 1
direction: direction/forward
top level: BBa_K106698
start: 6095
end: 6114
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999745
display id: annotation1999745
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999745/range1999745/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999745/range1999745/1
type: Range
persistent identity: range1999745
display id: range1999745
version: 1
direction: direction/forward
top level: BBa_K106698
start: 1
end: 4
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999742
display id: annotation1999742
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999742/range1999742/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999742/range1999742/1
type: Range
persistent identity: range1999742
display id: range1999742
version: 1
direction: direction/forward
top level: BBa_K106698
start: 5068
end: 5687
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999740
display id: annotation1999740
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999740/range1999740/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999740/range1999740/1
type: Range
persistent identity: range1999740
display id: range1999740
version: 1
direction: direction/forward
top level: BBa_K106698
start: 427
end: 733
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1995344
display id: annotation1995344
version: 1
top level: BBa_K106698
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995344/range1995344/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995344/range1995344/1
type: Range
persistent identity: range1995344
display id: range1995344
version: 1
direction: direction/forward
top level: BBa_K106698
start: 4053
end: 4913
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1995343
display id: annotation1995343
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995343/range1995343/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995343/range1995343/1
type: Range
persistent identity: range1995343
display id: range1995343
version: 1
direction: direction/reverse
top level: BBa_K106698
start: 3403
end: 3921
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1995347
display id: annotation1995347
version: 1
top level: BBa_K106698
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995347/range1995347/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995347/range1995347/1
type: Range
persistent identity: range1995347
display id: range1995347
version: 1
direction: direction/forward
top level: BBa_K106698
start: 22
end: 215
orientation: inline

Fig1 Promoter
type: SequenceAnnotation
persistent identity: annotation1995346
display id: annotation1995346
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995346/range1995346/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995346/range1995346/1
type: Range
persistent identity: range1995346
display id: range1995346
version: 1
direction: direction/forward
top level: BBa_K106698
start: 6300
end: 7309
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999738
display id: annotation1999738
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999738/range1999738/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999738/range1999738/1
type: Range
persistent identity: range1999738
display id: range1999738
version: 1
direction: direction/reverse
top level: BBa_K106698
start: 224
end: 242
orientation: inline

BBa_K2100081
type: ComponentDefinition
persistent identity: BBa K2100081
display id: BBa_K2100081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pEXPR Backbone
creator: Archis R. Bhandarkar
created: 2016-10-20T11:00:00Z
modified: 2016-10-21T08:51:37Z
discontinued: false
dominant: false
group u list: _2568_
m user id: 32435
owner id: 32435
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the plasmid backbone of an expression vector after performing an LR reaction in Gateway cloning assembly. It contains attB and attP sites that are the result of the recombination of attL and attR. The selection marker for this expression vector is ampicillin.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: attB
sequence: https://synbiohub.org/public/igem/BBa_K2100081_sequence/1

attB
type: SequenceAnnotation
persistent identity: annotation2527152
display id: annotation2527152
version: 1
top level: BBa_K2100081
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K2100081/annotation2527152/range2527152/1

https://synbiohub.org/public/igem/BBa_K2100081/annotation2527152/range2527152/1
type: Range
persistent identity: range2527152
display id: range2527152
version: 1
direction: direction/forward
top level: BBa_K2100081
start: 1
end: 21
orientation: inline

BBa_K1597001
type: ComponentDefinition
persistent identity: BBa K1597001
display id: BBa_K1597001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ThrC integration backbone for B. subtilis
creator: Marieke Mulder
created: 2015-09-08T11:00:00Z
modified: 2015-09-09T09:23:41Z
discontinued: false
dominant: false
group u list: _2014_
m user id: 24529
owner id: 24529
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Replication of tour bioBrick can via integration in B. subtilis locus. One of these loci is the ThrC locus. This backbone is based on the plasmids pdg1664 and K823023. The AmyE loci from K823023 were replaced with the ThrC loci from pdg1664. Along with this exchange, the chloramphenicol resistance gene was replaced with erythromycin resistant gene (wich was also found in the pdg1664). This biobrick can be replicated both in E. coli and in B. subtilis, where it intergrates into the genome.
mutable notes: pdg1664 contained an EcoRI and a XbaI restriction site. However these sites were situated around a locus. An option was to introduce the prefix, suffix and remove the other restriction site. Here is chosen to copy the loci and erythormycin resistance gene and use these to replace the AmyE intergration loci and chloremphencicol resistance gene from Bba_K823023.
mutable provenance: This backbone comes from combining Bba_K823023 and pdg1664
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: erythromycin resistance gene
sequence annotation: bla
sequence annotation: ThrC
sequence: https://synbiohub.org/public/igem/BBa_K1597001_sequence/1

erythromycin resistance gene
type: SequenceAnnotation
persistent identity: annotation2447870
display id: annotation2447870
version: 1
top level: BBa_K1597001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1597001/annotation2447870/range2447870/1

https://synbiohub.org/public/igem/BBa_K1597001/annotation2447870/range2447870/1
type: Range
persistent identity: range2447870
display id: range2447870
version: 1
direction: direction/forward
top level: BBa_K1597001
start: 334
end: 1105
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2447871
display id: annotation2447871
version: 1
top level: BBa_K1597001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1597001/annotation2447871/range2447871/1

https://synbiohub.org/public/igem/BBa_K1597001/annotation2447871/range2447871/1
type: Range
persistent identity: range2447871
display id: range2447871
version: 1
direction: direction/reverse
top level: BBa_K1597001
start: 3290
end: 4150
orientation: inline

ThrC
type: SequenceAnnotation
persistent identity: annotation2447868
display id: annotation2447868
version: 1
top level: BBa_K1597001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1597001/annotation2447868/range2447868/1

https://synbiohub.org/public/igem/BBa_K1597001/annotation2447868/range2447868/1
type: Range
persistent identity: range2447868
display id: range2447868
version: 1
direction: direction/forward
top level: BBa_K1597001
start: 1162
end: 1964
orientation: inline

ThrC
type: SequenceAnnotation
persistent identity: annotation2447869
display id: annotation2447869
version: 1
top level: BBa_K1597001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1597001/annotation2447869/range2447869/1

https://synbiohub.org/public/igem/BBa_K1597001/annotation2447869/range2447869/1
type: Range
persistent identity: range2447869
display id: range2447869
version: 1
direction: direction/forward
top level: BBa_K1597001
start: 4361
end: 5110
orientation: inline

K592202
type: ComponentDefinition
persistent identity: BBa K592202
display id: BBa_K592202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy Lambda Red recombineering compatible plasmid
creator: Antonio Ascue Avalos
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 10133
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K592202 is a BioBrick friendly vector with low to medium copy p15A replication origin (BBa_I50032) and a kanamycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22, and the kanamycin resistance marker is flanked by FRT sites so that the resistance marker can be removed after chromosomal integration using the FLP recombinase.
mutable notes: To make the plasmid usable for Lambda Red recombineering, all sequences with homologies to the E coli chromosome had to be removed. To accomplish this, the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. To be able to remove the antibiotic resistance marker after chromosomal integration, the kanamycin marker from the pKD4 plasmid (Datsenko and Wanner, 2000) was used. The resistance marker can be removed after chromosomal integration using the FLP recombinase, leaving only the plasmid insert and a scar behind.
mutable provenance: The P22 late terminator come from the genome of the Salmonella phage P22 (GenBank: AF217253.1, 19662..19702), and the kanamycin resistance marker flanked by FRT sites comes from the pKD4 plasmid (Datsenko and Wanner, 2000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Terminator BBa_B0054
sequence annotation: L-red_R_primer
sequence annotation: VR site BBa_G00102
sequence annotation: FRT site
sequence annotation: T3 terminator BBa_B0055
sequence annotation: P22 late terminator
sequence annotation: p15A ori BBa_I50032
sequence annotation: Translational stop BBa_B0042
sequence annotation: L-red_F_primer
sequence annotation: Terminator BBa_B0062
sequence annotation: VF2 site BBa_G00100
sequence annotation: Kanamycin resistance marker
sequence: https://synbiohub.org/public/igem/BBa_K592202_sequence/1

Terminator BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2137820
display id: annotation2137820
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137820/range2137820/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137820/range2137820/1
type: Range
persistent identity: range2137820
display id: range2137820
version: 1
direction: direction/forward
top level: K592202
start: 47
end: 115
orientation: inline

L-red_R_primer
type: SequenceAnnotation
persistent identity: annotation2137792
display id: annotation2137792
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137792/range2137792/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137792/range2137792/1
type: Range
persistent identity: range2137792
display id: range2137792
version: 1
direction: direction/reverse
top level: K592202
start: 20
end: 41
orientation: inline

VR site BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2137821
display id: annotation2137821
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137821/range2137821/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137821/range2137821/1
type: Range
persistent identity: range2137821
display id: range2137821
version: 1
direction: direction/reverse
top level: K592202
start: 116
end: 135
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2137825
display id: annotation2137825
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137825/range2137825/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137825/range2137825/1
type: Range
persistent identity: range2137825
display id: range2137825
version: 1
direction: direction/forward
top level: K592202
start: 1781
end: 1826
orientation: inline

T3 terminator BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2137830
display id: annotation2137830
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137830/range2137830/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137830/range2137830/1
type: Range
persistent identity: range2137830
display id: range2137830
version: 1
direction: direction/forward
top level: K592202
start: 3306
end: 3383
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2137828
display id: annotation2137828
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137828/range2137828/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137828/range2137828/1
type: Range
persistent identity: range2137828
display id: range2137828
version: 1
direction: direction/forward
top level: K592202
start: 3245
end: 3285
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2137827
display id: annotation2137827
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137827/range2137827/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137827/range2137827/1
type: Range
persistent identity: range2137827
display id: range2137827
version: 1
direction: direction/forward
top level: K592202
start: 3172
end: 3219
orientation: inline

p15A ori BBa_I50032
type: SequenceAnnotation
persistent identity: annotation2137823
display id: annotation2137823
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137823/range2137823/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137823/range2137823/1
type: Range
persistent identity: range2137823
display id: range2137823
version: 1
direction: direction/forward
top level: K592202
start: 184
end: 1748
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2137804
display id: annotation2137804
version: 1
top level: K592202
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137804/range2137804/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137804/range2137804/1
type: Range
persistent identity: range2137804
display id: range2137804
version: 1
direction: direction/forward
top level: K592202
start: 35
end: 46
orientation: inline

L-red_F_primer
type: SequenceAnnotation
persistent identity: annotation2137824
display id: annotation2137824
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137824/range2137824/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137824/range2137824/1
type: Range
persistent identity: range2137824
display id: range2137824
version: 1
direction: direction/forward
top level: K592202
start: 1726
end: 1745
orientation: inline

Terminator BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2137822
display id: annotation2137822
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137822/range2137822/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137822/range2137822/1
type: Range
persistent identity: range2137822
display id: range2137822
version: 1
direction: direction/forward
top level: K592202
start: 136
end: 176
orientation: inline

VF2 site BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2137829
display id: annotation2137829
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137829/range2137829/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137829/range2137829/1
type: Range
persistent identity: range2137829
display id: range2137829
version: 1
direction: direction/forward
top level: K592202
start: 3286
end: 3305
orientation: inline

Kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2137826
display id: annotation2137826
version: 1
top level: K592202
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137826/range2137826/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137826/range2137826/1
type: Range
persistent identity: range2137826
display id: range2137826
version: 1
direction: direction/reverse
top level: K592202
start: 2017
end: 2811
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2137846
display id: annotation2137846
version: 1
top level: K592202
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137846/range2137846/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137846/range2137846/1
type: Range
persistent identity: range2137846
display id: range2137846
version: 1
direction: direction/forward
top level: K592202
start: 3384
end: 3395
orientation: inline

BBa_K260003
type: ComponentDefinition
persistent identity: BBa K260003
display id: BBa_K260003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRhaFlp. [L-rhamnose] -> FLP(F70L)
creator: Kaj Bernhardt
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _356_
m user id: 0
owner id: 1072
owning group id: 57
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This plasmid is based on pSC101 and contains the rhamnose operon (rhaR and rhaS genes) as well as the F70L variant of FLP recombinase under rhamnose-promoter control. Therefore L-rhamnose can be used to induce FLP(F70L) expression. The pSC101 origin of replication is temperature sensitive due to a mutation of repA. Permisive temperature is 30 degC. 37 degC should be avoided as the plasmid can not replicate.
mutable notes: This plasmid comes from Sarov M, Schneider S, Pozniakovski A, Roguev A, Ernst S, Zhang Y, Hyman AA, Stewart AF. A recombineering pipeline for functional genomics applied to Caenorhabditis elegans. Nat Methods. 2006 Oct;3(10):839-44. However, it was modified by deletion of dhfr gene and RedABGRecA operon through insertion of a kanamycin resistance cassette.
mutable provenance: dhfr had to be deleted as the same gene for trimpethoprim resistance was going to be used elsewhere in the same cell.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pSC101 origin
sequence annotation: T4_32 terminator
sequence annotation: rhaS
sequence annotation: rhamnose-promoter
sequence annotation: KanR
sequence annotation: suffix
sequence annotation: repA
sequence annotation: rhaR
sequence annotation: prefix
sequence annotation: FLP(F70L)
sequence: https://synbiohub.org/public/igem/BBa_K260003_sequence/1

pSC101 origin
type: SequenceAnnotation
persistent identity: annotation2048724
display id: annotation2048724
version: 1
top level: BBa_K260003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260003/annotation2048724/range2048724/1

https://synbiohub.org/public/igem/BBa_K260003/annotation2048724/range2048724/1
type: Range
persistent identity: range2048724
display id: range2048724
version: 1
direction: direction/reverse
top level: BBa_K260003
start: 1442
end: 1803
orientation: inline

T4_32 terminator
type: SequenceAnnotation
persistent identity: annotation2048730
display id: annotation2048730
version: 1
top level: BBa_K260003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K260003/annotation2048730/range2048730/1

https://synbiohub.org/public/igem/BBa_K260003/annotation2048730/range2048730/1
type: Range
persistent identity: range2048730
display id: range2048730
version: 1
direction: direction/forward
top level: BBa_K260003
start: 6272
end: 6303
orientation: inline

rhaS
type: SequenceAnnotation
persistent identity: annotation2048728
display id: annotation2048728
version: 1
top level: BBa_K260003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260003/annotation2048728/range2048728/1

https://synbiohub.org/public/igem/BBa_K260003/annotation2048728/range2048728/1
type: Range
persistent identity: range2048728
display id: range2048728
version: 1
direction: direction/forward
top level: BBa_K260003
start: 4513
end: 5349
orientation: inline

rhamnose-promoter
type: SequenceAnnotation
persistent identity: annotation2048732
display id: annotation2048732
version: 1
top level: BBa_K260003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K260003/annotation2048732/range2048732/1

https://synbiohub.org/public/igem/BBa_K260003/annotation2048732/range2048732/1
type: Range
persistent identity: range2048732
display id: range2048732
version: 1
direction: direction/reverse
top level: BBa_K260003
start: 4226
end: 4512
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2048725
display id: annotation2048725
version: 1
top level: BBa_K260003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260003/annotation2048725/range2048725/1

https://synbiohub.org/public/igem/BBa_K260003/annotation2048725/range2048725/1
type: Range
persistent identity: range2048725
display id: range2048725
version: 1
direction: direction/reverse
top level: BBa_K260003
start: 1854
end: 2648
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2048722
display id: annotation2048722
version: 1
top level: BBa_K260003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260003/annotation2048722/range2048722/1

https://synbiohub.org/public/igem/BBa_K260003/annotation2048722/range2048722/1
type: Range
persistent identity: range2048722
display id: range2048722
version: 1
direction: direction/forward
top level: BBa_K260003
start: 1
end: 21
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2048723
display id: annotation2048723
version: 1
top level: BBa_K260003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260003/annotation2048723/range2048723/1

https://synbiohub.org/public/igem/BBa_K260003/annotation2048723/range2048723/1
type: Range
persistent identity: range2048723
display id: range2048723
version: 1
direction: direction/reverse
top level: BBa_K260003
start: 108
end: 1058
orientation: inline

rhaR
type: SequenceAnnotation
persistent identity: annotation2048729
display id: annotation2048729
version: 1
top level: BBa_K260003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260003/annotation2048729/range2048729/1

https://synbiohub.org/public/igem/BBa_K260003/annotation2048729/range2048729/1
type: Range
persistent identity: range2048729
display id: range2048729
version: 1
direction: direction/forward
top level: BBa_K260003
start: 5333
end: 6271
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2048733
display id: annotation2048733
version: 1
top level: BBa_K260003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260003/annotation2048733/range2048733/1

https://synbiohub.org/public/igem/BBa_K260003/annotation2048733/range2048733/1
type: Range
persistent identity: range2048733
display id: range2048733
version: 1
direction: direction/forward
top level: BBa_K260003
start: 6324
end: 6345
orientation: inline

T4_32 terminator
type: SequenceAnnotation
persistent identity: annotation2048726
display id: annotation2048726
version: 1
top level: BBa_K260003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K260003/annotation2048726/range2048726/1

https://synbiohub.org/public/igem/BBa_K260003/annotation2048726/range2048726/1
type: Range
persistent identity: range2048726
display id: range2048726
version: 1
direction: direction/reverse
top level: BBa_K260003
start: 2902
end: 2933
orientation: inline

FLP(F70L)
type: SequenceAnnotation
persistent identity: annotation2048727
display id: annotation2048727
version: 1
top level: BBa_K260003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260003/annotation2048727/range2048727/1

https://synbiohub.org/public/igem/BBa_K260003/annotation2048727/range2048727/1
type: Range
persistent identity: range2048727
display id: range2048727
version: 1
direction: direction/reverse
top level: BBa_K260003
start: 2954
end: 4225
orientation: inline

BBa_K814015
type: ComponentDefinition
persistent identity: BBa K814015
display id: BBa_K814015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: G418 selection yeast shuttle vector designed for BioBrick
creator: George Chao, Misha Patel, Hannah Aho, Molly Swanson, Dana Morrone
created: 2012-10-02T11:00:00Z
modified: 2015-05-08T01:13:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1071_
m user id: 0
owner id: 9070
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A yeast/E. coli shuttle vector was designed with BioBrick prefix and suffix sites such that a yeast expression cassette could be inserted through basic BioBrick assembly. G418 was chosen instead of an auxotrophic marker for plasmid selection in yeast. To synthesize the overall plasmid backbone, PCR primers were designed to amplify five desired fragments (with 25-30 bp overlap) from different plasmids, which could be joined by Gibson Assembly: 1. BioBrick destination plasmid pSB1C3 containing the MCS and rep (pMB1). 2. BioBrick destination plasmid pSB1C3 containing the Chloramphenical resistance gene. 3. 2u Gibson Extract provided by the Schmidt-Dannert lab (University of Minnesota) containing the 2u ORI for yeast. 4. pkT127 provided by the Schmidt-Dannert lab containing G418 resistance genes and tTEF1. 5. ATCC plasmid provided by the Schmidt-Dannert lab containing the ADH1 promoter to drive the G418 R gene.
mutable notes: A number of mutations were directed so as to maintin the uniqueness of BioBrick cut sites.
mutable provenance: This plasmid was assembled though Gibson assembly. Gibson assembly pieces were amplified from preexisting plasmid templates.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: stem_loop
sequence annotation: Yeast Kozak
sequence annotation: Chloramphenicol resistance
sequence annotation: rep (pBM1)
sequence annotation: pADH1
sequence annotation: 2u Yeast ORI
sequence annotation: BioBrick suffix
sequence annotation: VR
sequence annotation: G418 resistance marker
sequence annotation: VF2
sequence annotation: T0 terminator
sequence annotation: His operon terminator
sequence annotation: tTEF1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K814015_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2211693
display id: annotation2211693
version: 1
top level: BBa_K814015
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211693/range2211693/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211693/range2211693/1
type: Range
persistent identity: range2211693
display id: range2211693
version: 1
direction: direction/forward
top level: BBa_K814015
start: 5654
end: 5673
orientation: inline

Yeast Kozak
type: SequenceAnnotation
persistent identity: annotation2211689
display id: annotation2211689
version: 1
top level: BBa_K814015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211689/range2211689/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211689/range2211689/1
type: Range
persistent identity: range2211689
display id: range2211689
version: 1
direction: direction/reverse
top level: BBa_K814015
start: 4070
end: 4075
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2211685
display id: annotation2211685
version: 1
top level: BBa_K814015
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211685/range2211685/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211685/range2211685/1
type: Range
persistent identity: range2211685
display id: range2211685
version: 1
direction: direction/reverse
top level: BBa_K814015
start: 1043
end: 1703
orientation: inline

rep (pBM1)
type: SequenceAnnotation
persistent identity: annotation2211683
display id: annotation2211683
version: 1
top level: BBa_K814015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211683/range2211683/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211683/range2211683/1
type: Range
persistent identity: range2211683
display id: range2211683
version: 1
direction: direction/forward
top level: BBa_K814015
start: 260
end: 614
orientation: inline

pADH1
type: SequenceAnnotation
persistent identity: annotation2211690
display id: annotation2211690
version: 1
top level: BBa_K814015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211690/range2211690/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211690/range2211690/1
type: Range
persistent identity: range2211690
display id: range2211690
version: 1
direction: direction/reverse
top level: BBa_K814015
start: 4095
end: 5539
orientation: inline

2u Yeast ORI
type: SequenceAnnotation
persistent identity: annotation2211686
display id: annotation2211686
version: 1
top level: BBa_K814015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211686/range2211686/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211686/range2211686/1
type: Range
persistent identity: range2211686
display id: range2211686
version: 1
direction: direction/forward
top level: BBa_K814015
start: 1803
end: 3009
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2211681
display id: annotation2211681
version: 1
top level: BBa_K814015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211681/range2211681/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211681/range2211681/1
type: Range
persistent identity: range2211681
display id: range2211681
version: 1
direction: direction/forward
top level: BBa_K814015
start: 1
end: 20
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2211691
display id: annotation2211691
version: 1
top level: BBa_K814015
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211691/range2211691/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211691/range2211691/1
type: Range
persistent identity: range2211691
display id: range2211691
version: 1
direction: direction/reverse
top level: BBa_K814015
start: 155
end: 170
orientation: inline

G418 resistance marker
type: SequenceAnnotation
persistent identity: annotation2211688
display id: annotation2211688
version: 1
top level: BBa_K814015
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211688/range2211688/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211688/range2211688/1
type: Range
persistent identity: range2211688
display id: range2211688
version: 1
direction: direction/reverse
top level: BBa_K814015
start: 3260
end: 4069
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2211692
display id: annotation2211692
version: 1
top level: BBa_K814015
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211692/range2211692/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211692/range2211692/1
type: Range
persistent identity: range2211692
display id: range2211692
version: 1
direction: direction/forward
top level: BBa_K814015
start: 5565
end: 5583
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2211684
display id: annotation2211684
version: 1
top level: BBa_K814015
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211684/range2211684/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211684/range2211684/1
type: Range
persistent identity: range2211684
display id: range2211684
version: 1
direction: direction/reverse
top level: BBa_K814015
start: 926
end: 1031
orientation: inline

His operon terminator
type: SequenceAnnotation
persistent identity: annotation2211682
display id: annotation2211682
version: 1
top level: BBa_K814015
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211682/range2211682/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211682/range2211682/1
type: Range
persistent identity: range2211682
display id: range2211682
version: 1
direction: direction/forward
top level: BBa_K814015
start: 21
end: 91
orientation: inline

tTEF1 terminator
type: SequenceAnnotation
persistent identity: annotation2211687
display id: annotation2211687
version: 1
top level: BBa_K814015
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K814015/annotation2211687/range2211687/1

https://synbiohub.org/public/igem/BBa_K814015/annotation2211687/range2211687/1
type: Range
persistent identity: range2211687
display id: range2211687
version: 1
direction: direction/reverse
top level: BBa_K814015
start: 3035
end: 3259
orientation: inline

BBa_K929300
type: ComponentDefinition
persistent identity: BBa K929300
display id: BBa_K929300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Potsdam Standard Backbone
creator: iGEM Potsdam Bioware 2012
created: 2012-09-23T11:00:00Z
is replaced by: https://synbiohub.org/public/igem/BBa_K929301/1
modified: 2015-05-08T01:13:47Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1194_
m user id: 0
owner id: 14525
owning group id: 9
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: The Potsdam Assembly Standard is a modified PLICing (Phosphorothioate-based ligase-independent gene cloning) method ([http://www.ncbi.nlm.nih.gov/pubmed/20646988 Blanusa ''et al.'' (2010)]). For this standard we designed a new cloning backbone with an RFP expression cassette as insert and two new restriction enzyme recognition sites in the suffix and prefix in pSB1C3, the Potsdam Standard Backbone. In the prefix, we added the Apa I recognition site and in the suffix the Sph I recognition site. Both enzymes causing a 3??? overhang with 4 nucleotides. For the cloning process we cut the vector with Apa I and Sph I. In this assembly standard that???s the only step where we have to use restriction enzymes. The gene of interest was amplified with primers that contain 4 phosphothioate nucleotides and the recognition sites for Apa I and Sph I at the 5??? end. After incubation in an iodine/ethanol solution, the thiophosphates were cut out resulting in a 3??? overhang which is suitable to the overhangs that was created by cutting the new assembly vector. After that the digested vector and the PLICed insert were mixed and transformed into E. coli. By using the RFP expression cassette we created a ligation control system. Due to the fact that red fluorescent colonies have a failed vector ligation we can tell which colonies are correctly ligated. The colonies that do not show red fluorescence are the positive clones.
mutable notes: To create the Potsdam Standard Backbone, we amplified the RFP expression cassette (BBa_J04450) to add the restriction site for Apa I and Sph I. Then, the amplified expression cassette was insert into pSB1C3 using Xba I and Pst I.
mutable provenance: BBa_J04450
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K929300_sequence/1

BBa_K590013
type: ComponentDefinition
persistent identity: BBa K590013
display id: BBa_K590013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGA4A5, Gibson assembly plasmid (bglBrick) with pLac-GFP insert
creator: Michael Brasino, Alicia Wong, Rashmi Ravichandran
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Backbone that is Gibson reaction friendly (Bgl Brick)
mutable notes: sequence confirmed
mutable provenance: Made from Gibson reaction
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K590013_sequence/1

pSB3T5
type: ComponentDefinition
persistent identity: pSB3T5
display id: pSB3T5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3T5 is a low to medium copy plasmid based with tetracycline resistance. It has a p15A-derived replication origin. pSB3T5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the tetracycline resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0042
component: BBa_G0002
component: BBa_G0003
component: BBa_G00102
component: BBa_B0055
component: BBa_G00100
component: BBa_G00001
component: BBa_B0043
component: BBa_P1005
component: BBa_I50032
component: BBa_B0054
component: BBa_G00000
component: BBa_B0053
component: BBa_G0004
component: BBa_B0062
component: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_B0044
sequence annotation: BBa_B0055
sequence annotation: BBa_P1005
sequence annotation: BBa_G00100
sequence annotation: BBa_G0002
sequence annotation: BBa_I50032
sequence annotation: BBa_B0062
sequence annotation: BBa_G00102
sequence annotation: BBa_B0043
sequence annotation: BBa_B0053
sequence annotation: BBa_G0003
sequence annotation: BBa_B0054
sequence annotation: BBa_G00001
sequence annotation: BBa_G00000
sequence annotation: BBa_G0004
sequence: https://synbiohub.org/public/igem/pSB3T5_sequence/1

BBa_B0042
type: Component
persistent identity: component1928398
display id: component1928398
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0002
type: Component
persistent identity: component1928381
display id: component1928381
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0003
type: Component
persistent identity: component1928377
display id: component1928377
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00102
type: Component
persistent identity: component1928374
display id: component1928374
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component1928370
display id: component1928370
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0055
type: Component
persistent identity: component1928391
display id: component1928391
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00100
type: Component
persistent identity: component1928388
display id: component1928388
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00001
type: Component
persistent identity: component1928358
display id: component1928358
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0043
type: Component
persistent identity: component1928403
display id: component1928403
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_P1005
type: Component
persistent identity: component1928384
display id: component1928384
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1005/1

BBa_I50032
type: Component
persistent identity: component1928380
display id: component1928380
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_B0054
type: Component
persistent identity: component1928373
display id: component1928373
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00000
type: Component
persistent identity: component1928405
display id: component1928405
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0053
type: Component
persistent identity: component1928387
display id: component1928387
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0004
type: Component
persistent identity: component1928385
display id: component1928385
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0062
type: Component
persistent identity: component1928376
display id: component1928376
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0044
type: Component
persistent identity: component1928363
display id: component1928363
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928398
display id: annotation1928398
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928398/range1928398/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3T5/annotation1928398/range1928398/1
type: Range
persistent identity: range1928398
display id: range1928398
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3206
end: 3217
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1928363
display id: annotation1928363
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928363/range1928363/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3T5/annotation1928363/range1928363/1
type: Range
persistent identity: range1928363
display id: range1928363
version: 1
direction: direction/reverse
top level: pSB3T5
start: 22
end: 34
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1928391
display id: annotation1928391
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928391/range1928391/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3T5/annotation1928391/range1928391/1
type: Range
persistent identity: range1928391
display id: range1928391
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3128
end: 3205
orientation: inline

BBa_P1005
type: SequenceAnnotation
persistent identity: annotation1928384
display id: annotation1928384
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928384/range1928384/1
component: BBa_P1005

https://synbiohub.org/public/igem/pSB3T5/annotation1928384/range1928384/1
type: Range
persistent identity: range1928384
display id: range1928384
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1746
end: 3028
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1928388
display id: annotation1928388
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928388/range1928388/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3T5/annotation1928388/range1928388/1
type: Range
persistent identity: range1928388
display id: range1928388
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3108
end: 3127
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1928381
display id: annotation1928381
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928381/range1928381/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3T5/annotation1928381/range1928381/1
type: Range
persistent identity: range1928381
display id: range1928381
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1738
end: 1745
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1928380
display id: annotation1928380
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928380/range1928380/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3T5/annotation1928380/range1928380/1
type: Range
persistent identity: range1928380
display id: range1928380
version: 1
direction: direction/reverse
top level: pSB3T5
start: 184
end: 1737
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1928376
display id: annotation1928376
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928376/range1928376/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3T5/annotation1928376/range1928376/1
type: Range
persistent identity: range1928376
display id: range1928376
version: 1
direction: direction/reverse
top level: pSB3T5
start: 136
end: 176
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1928374
display id: annotation1928374
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928374/range1928374/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3T5/annotation1928374/range1928374/1
type: Range
persistent identity: range1928374
display id: range1928374
version: 1
direction: direction/reverse
top level: pSB3T5
start: 116
end: 135
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928370
display id: annotation1928370
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928370/range1928370/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3T5/annotation1928370/range1928370/1
type: Range
persistent identity: range1928370
display id: range1928370
version: 1
direction: direction/reverse
top level: pSB3T5
start: 35
end: 46
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1928403
display id: annotation1928403
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928403/range1928403/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3T5/annotation1928403/range1928403/1
type: Range
persistent identity: range1928403
display id: range1928403
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3218
end: 3230
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1928387
display id: annotation1928387
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928387/range1928387/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3T5/annotation1928387/range1928387/1
type: Range
persistent identity: range1928387
display id: range1928387
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3036
end: 3107
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1928377
display id: annotation1928377
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928377/range1928377/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3T5/annotation1928377/range1928377/1
type: Range
persistent identity: range1928377
display id: range1928377
version: 1
direction: direction/reverse
top level: pSB3T5
start: 177
end: 183
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1928373
display id: annotation1928373
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928373/range1928373/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3T5/annotation1928373/range1928373/1
type: Range
persistent identity: range1928373
display id: range1928373
version: 1
direction: direction/reverse
top level: pSB3T5
start: 47
end: 115
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1928358
display id: annotation1928358
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928358/range1928358/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3T5/annotation1928358/range1928358/1
type: Range
persistent identity: range1928358
display id: range1928358
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1
end: 21
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1928405
display id: annotation1928405
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928405/range1928405/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3T5/annotation1928405/range1928405/1
type: Range
persistent identity: range1928405
display id: range1928405
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3231
end: 3252
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1928385
display id: annotation1928385
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928385/range1928385/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3T5/annotation1928385/range1928385/1
type: Range
persistent identity: range1928385
display id: range1928385
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3029
end: 3035
orientation: inline

BBa_K799024
type: ComponentDefinition
persistent identity: BBa K799024
display id: BBa_K799024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3 yGG promoter acceptor vector
creator: Daniel Wolozny
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1055_
m user id: 0
owner id: 8447
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The original psb1c3 backbone was modified in order to allow for Golden Gate enabled parts to easily become biobricks.
mutable notes: Golden Gate modified.
mutable provenance: Igem Registry of Standard Biological Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Psb1C3 modified backbone
sequence: https://synbiohub.org/public/igem/BBa_K799024_sequence/1

Psb1C3 modified backbone
type: SequenceAnnotation
persistent identity: annotation2197851
display id: annotation2197851
version: 1
top level: BBa_K799024
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K799024/annotation2197851/range2197851/1

https://synbiohub.org/public/igem/BBa_K799024/annotation2197851/range2197851/1
type: Range
persistent identity: range2197851
display id: range2197851
version: 1
direction: direction/forward
top level: BBa_K799024
start: 1
end: 2027
orientation: inline

BBa_K635002
type: ComponentDefinition
persistent identity: BBa K635002
display id: BBa_K635002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artificial Yeast Chromosome (Ura3 & Amp)
creator: Arjun Athreya
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _810_
m user id: 0
owner id: 7191
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part serves as a foundational contribution to expand the registry of yeast components. It is an easy to use artificial yeast chromosome with an auxitrophic uracil selection method for yeast expression, as well as an ampicillin selection for propagation in E.coli. The part was modified for biobrick compatibility from a working pRS316 plasmid containing a Gal1/10 bidirectional promoter which was removed through restriction digest. In addition to the biobrick prefix and suffix, the backbone contains Kpn1 and Sac1 restriction sites the flank the prefix and suffix respectively. Yeast transformants can be selected by using a uracil drop out medium and E.coli transformants may be selected using ampicillin resistance.
mutable notes: The plasmid backbone contained a Gal1/10 bidirectional promoter between the EcoR1 and BamHI sites on the pRS316 plasmid. The plasmid had to be modified for biobrick compatibility by using the HindIII and SacI sites to digest and ligate a gene fragment with the corresponding HindIII and SacI sites, but which also contained the biobrick prefix and suffix in the correct orientation.
mutable provenance: The part was modified for biobrick compatibility from a working pRS316 plasmid containing a Gal1/10 bidirectional promoter which was removed through restriction digest.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: psBR322 origin
sequence annotation: Ampicillin resistance
sequence annotation: prefix
sequence annotation: CEN/ARS
sequence annotation: URA3
sequence annotation: suffix
sequence: https://synbiohub.org/public/igem/BBa_K635002_sequence/1

psBR322 origin
type: SequenceAnnotation
persistent identity: annotation2144982
display id: annotation2144982
version: 1
top level: BBa_K635002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K635002/annotation2144982/range2144982/1

https://synbiohub.org/public/igem/BBa_K635002/annotation2144982/range2144982/1
type: Range
persistent identity: range2144982
display id: range2144982
version: 1
direction: direction/forward
top level: BBa_K635002
start: 3749
end: 4368
orientation: inline

Ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation2144981
display id: annotation2144981
version: 1
top level: BBa_K635002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K635002/annotation2144981/range2144981/1

https://synbiohub.org/public/igem/BBa_K635002/annotation2144981/range2144981/1
type: Range
persistent identity: range2144981
display id: range2144981
version: 1
direction: direction/reverse
top level: BBa_K635002
start: 2734
end: 3594
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2144962
display id: annotation2144962
version: 1
top level: BBa_K635002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K635002/annotation2144962/range2144962/1

https://synbiohub.org/public/igem/BBa_K635002/annotation2144962/range2144962/1
type: Range
persistent identity: range2144962
display id: range2144962
version: 1
direction: direction/reverse
top level: BBa_K635002
start: 4849
end: 4870
orientation: inline

CEN/ARS
type: SequenceAnnotation
persistent identity: annotation2144980
display id: annotation2144980
version: 1
top level: BBa_K635002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K635002/annotation2144980/range2144980/1

https://synbiohub.org/public/igem/BBa_K635002/annotation2144980/range2144980/1
type: Range
persistent identity: range2144980
display id: range2144980
version: 1
direction: direction/reverse
top level: BBa_K635002
start: 2084
end: 2602
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2144979
display id: annotation2144979
version: 1
top level: BBa_K635002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K635002/annotation2144979/range2144979/1

https://synbiohub.org/public/igem/BBa_K635002/annotation2144979/range2144979/1
type: Range
persistent identity: range2144979
display id: range2144979
version: 1
direction: direction/reverse
top level: BBa_K635002
start: 1611
end: 1824
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2144960
display id: annotation2144960
version: 1
top level: BBa_K635002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K635002/annotation2144960/range2144960/1

https://synbiohub.org/public/igem/BBa_K635002/annotation2144960/range2144960/1
type: Range
persistent identity: range2144960
display id: range2144960
version: 1
direction: direction/reverse
top level: BBa_K635002
start: 1
end: 21
orientation: inline

BBa_K1138001
type: ComponentDefinition
persistent identity: BBa K1138001
display id: BBa_K1138001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAW50-linker-mcherry(-RBS) (ugaiGEM11)
creator: Rachit Jain
created: 2013-09-05T11:00:00Z
modified: 2015-05-08T01:09:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1450_
m user id: 0
owner id: 12767
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This plasmid consists of the mcherry gene (encoding red fluorescence protein) inserted in the backbone of pAW50 vector. EcoRI-NotI-XbaI-NsiI restriction sites have been introduced followed by a linker sequence followed by the mcherry sequence. The use of these sites will allow users to introduce any desired gene of their choice to be fused with the linker which is already fused with the mcherry sequence. This way any protein can be qualitatively studied in Methanogens via red fluorescence. Note:- the users will have to introduce the RBS sequence and start codon, delete the terminator sequence of their gene while cloning.
mutable notes: Primer were designed in a way to introduce EcoRI-NotI-XbaI-NsiI sites before the linker.
mutable provenance: mcherry was obtained from Discosoma sp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ampicillin
sequence annotation: NotI
sequence annotation: mcherry
sequence annotation: promoter
sequence annotation: NsiI
sequence annotation: puramycin
sequence annotation: EcoRI
sequence annotation: XbaI
sequence annotation: linker sequence
sequence: https://synbiohub.org/public/igem/BBa_K1138001_sequence/1

ampicillin
type: SequenceAnnotation
persistent identity: annotation2335220
display id: annotation2335220
version: 1
top level: BBa_K1138001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138001/annotation2335220/range2335220/1

https://synbiohub.org/public/igem/BBa_K1138001/annotation2335220/range2335220/1
type: Range
persistent identity: range2335220
display id: range2335220
version: 1
direction: direction/forward
top level: BBa_K1138001
start: 2430
end: 3290
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation2335214
display id: annotation2335214
version: 1
top level: BBa_K1138001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138001/annotation2335214/range2335214/1

https://synbiohub.org/public/igem/BBa_K1138001/annotation2335214/range2335214/1
type: Range
persistent identity: range2335214
display id: range2335214
version: 1
direction: direction/forward
top level: BBa_K1138001
start: 211
end: 218
orientation: inline

mcherry
type: SequenceAnnotation
persistent identity: annotation2335218
display id: annotation2335218
version: 1
top level: BBa_K1138001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1138001/annotation2335218/range2335218/1

https://synbiohub.org/public/igem/BBa_K1138001/annotation2335218/range2335218/1
type: Range
persistent identity: range2335218
display id: range2335218
version: 1
direction: direction/forward
top level: BBa_K1138001
start: 261
end: 968
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2335212
display id: annotation2335212
version: 1
top level: BBa_K1138001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1138001/annotation2335212/range2335212/1

https://synbiohub.org/public/igem/BBa_K1138001/annotation2335212/range2335212/1
type: Range
persistent identity: range2335212
display id: range2335212
version: 1
direction: direction/forward
top level: BBa_K1138001
start: 18
end: 204
orientation: inline

NsiI
type: SequenceAnnotation
persistent identity: annotation2335216
display id: annotation2335216
version: 1
top level: BBa_K1138001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138001/annotation2335216/range2335216/1

https://synbiohub.org/public/igem/BBa_K1138001/annotation2335216/range2335216/1
type: Range
persistent identity: range2335216
display id: range2335216
version: 1
direction: direction/forward
top level: BBa_K1138001
start: 227
end: 232
orientation: inline

puramycin
type: SequenceAnnotation
persistent identity: annotation2335219
display id: annotation2335219
version: 1
top level: BBa_K1138001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138001/annotation2335219/range2335219/1

https://synbiohub.org/public/igem/BBa_K1138001/annotation2335219/range2335219/1
type: Range
persistent identity: range2335219
display id: range2335219
version: 1
direction: direction/forward
top level: BBa_K1138001
start: 1048
end: 1647
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2335213
display id: annotation2335213
version: 1
top level: BBa_K1138001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138001/annotation2335213/range2335213/1

https://synbiohub.org/public/igem/BBa_K1138001/annotation2335213/range2335213/1
type: Range
persistent identity: range2335213
display id: range2335213
version: 1
direction: direction/forward
top level: BBa_K1138001
start: 205
end: 210
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2335215
display id: annotation2335215
version: 1
top level: BBa_K1138001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138001/annotation2335215/range2335215/1

https://synbiohub.org/public/igem/BBa_K1138001/annotation2335215/range2335215/1
type: Range
persistent identity: range2335215
display id: range2335215
version: 1
direction: direction/forward
top level: BBa_K1138001
start: 220
end: 225
orientation: inline

linker sequence
type: SequenceAnnotation
persistent identity: annotation2335217
display id: annotation2335217
version: 1
top level: BBa_K1138001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138001/annotation2335217/range2335217/1

https://synbiohub.org/public/igem/BBa_K1138001/annotation2335217/range2335217/1
type: Range
persistent identity: range2335217
display id: range2335217
version: 1
direction: direction/forward
top level: BBa_K1138001
start: 233
end: 260
orientation: inline

pBBknock
type: ComponentDefinition
persistent identity: BBa J107077
display id: BBa_J107077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBBknock standard vector for allelic replacement
creator: Michela Casanova, Lorenzo Pasotti, Susanna Zucca, Nicolo Politi, Ilaria Massaiu, Cinzia Calvio, Gabriella Cusella, Paolo Magni
created: 2016-01-02T12:00:00Z
modified: 2016-02-11T02:50:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _587_
m user id: 2621
owner id: 2621
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This vector can be specialized to target the desired locus in the Escherichia coli genome and carry out allelic replacement, e.g., knockout, chromosomal integration, mutagenesis, etc. See Casanova et al. (2016), Biol Proced Online for detailed instructions and protocols.
mutable notes: See BBa_J107112.
mutable provenance: Synthetic DNA obtained via GenScript synthesis service.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_J23101
component: BBa_I766205
component: BBa_G0002
component: BBa_J107112
component: BBa_J107113
component: BBa_M31985
component: BBa_G00001
component: BBa_G00000
component: BBa_M300055
component: BBa_K300999
component: BBa_B0046
component: BBa_J107114
component: BBa_J33204
sequence annotation: BBa_G00001
sequence annotation: BBa_G0002
sequence annotation: BBa_J107113
sequence annotation: BBa_J107112
sequence annotation: BBa_I766205
sequence annotation: BBa_K300999
sequence annotation: BBa_M31985
sequence annotation: BBa_B0046
sequence annotation: BBa_J23101
sequence annotation: BBa_M300055
sequence annotation: BBa_G00000
sequence annotation: BBa_J107114
sequence annotation: BBa_J33204
sequence: https://synbiohub.org/public/igem/BBa_J107077_sequence/1

BBa_J23101
type: Component
persistent identity: component2478497
display id: component2478497
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_I766205
type: Component
persistent identity: component2478510
display id: component2478510
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_I766205/1

BBa_G0002
type: Component
persistent identity: component2478498
display id: component2478498
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_J107112
type: Component
persistent identity: component2478506
display id: component2478506
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_J107112/1

BBa_J107113
type: Component
persistent identity: component2478503
display id: component2478503
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_J107113/1

BBa_M31985
type: Component
persistent identity: component2478502
display id: component2478502
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_M31985/1

BBa_G00001
type: Component
persistent identity: component2478496
display id: component2478496
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00000
type: Component
persistent identity: component2478517
display id: component2478517
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_M300055
type: Component
persistent identity: component2478505
display id: component2478505
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_M300055/1

BBa_K300999
type: Component
persistent identity: component2478512
display id: component2478512
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_K300999/1

BBa_B0046
type: Component
persistent identity: component2478508
display id: component2478508
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_B0046/1

BBa_J107114
type: Component
persistent identity: component2478509
display id: component2478509
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_J107114/1

BBa_J33204
type: Component
persistent identity: component2478501
display id: component2478501
version: 1
top level: pBBknock
access: public
definition: https://synbiohub.org/public/igem/BBa_J33204/1

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2478496
display id: annotation2478496
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478496/range2478496/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_J107077/annotation2478496/range2478496/1
type: Range
persistent identity: range2478496
display id: range2478496
version: 1
direction: direction/reverse
top level: pBBknock
start: 1
end: 21
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2478498
display id: annotation2478498
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478498/range2478498/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_J107077/annotation2478498/range2478498/1
type: Range
persistent identity: range2478498
display id: range2478498
version: 1
direction: direction/reverse
top level: pBBknock
start: 57
end: 64
orientation: inline

BBa_J107113
type: SequenceAnnotation
persistent identity: annotation2478503
display id: annotation2478503
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478503/range2478503/1
component: BBa_J107113

https://synbiohub.org/public/igem/BBa_J107077/annotation2478503/range2478503/1
type: Range
persistent identity: range2478503
display id: range2478503
version: 1
direction: direction/reverse
top level: pBBknock
start: 1029
end: 1077
orientation: inline

BBa_J107112
type: SequenceAnnotation
persistent identity: annotation2478506
display id: annotation2478506
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478506/range2478506/1
component: BBa_J107112

https://synbiohub.org/public/igem/BBa_J107077/annotation2478506/range2478506/1
type: Range
persistent identity: range2478506
display id: range2478506
version: 1
direction: direction/reverse
top level: pBBknock
start: 1084
end: 3026
orientation: inline

BBa_I766205
type: SequenceAnnotation
persistent identity: annotation2478510
display id: annotation2478510
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478510/range2478510/1
component: BBa_I766205

https://synbiohub.org/public/igem/BBa_J107077/annotation2478510/range2478510/1
type: Range
persistent identity: range2478510
display id: range2478510
version: 1
direction: direction/reverse
top level: pBBknock
start: 3090
end: 3095
orientation: inline

BBa_K300999
type: SequenceAnnotation
persistent identity: annotation2478512
display id: annotation2478512
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478512/range2478512/1
component: BBa_K300999

https://synbiohub.org/public/igem/BBa_J107077/annotation2478512/range2478512/1
type: Range
persistent identity: range2478512
display id: range2478512
version: 1
direction: direction/reverse
top level: pBBknock
start: 3096
end: 3864
orientation: inline

BBa_M31985
type: SequenceAnnotation
persistent identity: annotation2478502
display id: annotation2478502
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478502/range2478502/1
component: BBa_M31985

https://synbiohub.org/public/igem/BBa_J107077/annotation2478502/range2478502/1
type: Range
persistent identity: range2478502
display id: range2478502
version: 1
direction: direction/reverse
top level: pBBknock
start: 1023
end: 1028
orientation: inline

BBa_B0046
type: SequenceAnnotation
persistent identity: annotation2478508
display id: annotation2478508
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478508/range2478508/1
component: BBa_B0046

https://synbiohub.org/public/igem/BBa_J107077/annotation2478508/range2478508/1
type: Range
persistent identity: range2478508
display id: range2478508
version: 1
direction: direction/reverse
top level: pBBknock
start: 3027
end: 3032
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2478497
display id: annotation2478497
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478497/range2478497/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_J107077/annotation2478497/range2478497/1
type: Range
persistent identity: range2478497
display id: range2478497
version: 1
direction: direction/reverse
top level: pBBknock
start: 22
end: 56
orientation: inline

BBa_M300055
type: SequenceAnnotation
persistent identity: annotation2478505
display id: annotation2478505
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478505/range2478505/1
component: BBa_M300055

https://synbiohub.org/public/igem/BBa_J107077/annotation2478505/range2478505/1
type: Range
persistent identity: range2478505
display id: range2478505
version: 1
direction: direction/reverse
top level: pBBknock
start: 1078
end: 1083
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2478517
display id: annotation2478517
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478517/range2478517/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_J107077/annotation2478517/range2478517/1
type: Range
persistent identity: range2478517
display id: range2478517
version: 1
direction: direction/reverse
top level: pBBknock
start: 3865
end: 3886
orientation: inline

BBa_J107114
type: SequenceAnnotation
persistent identity: annotation2478509
display id: annotation2478509
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478509/range2478509/1
component: BBa_J107114

https://synbiohub.org/public/igem/BBa_J107077/annotation2478509/range2478509/1
type: Range
persistent identity: range2478509
display id: range2478509
version: 1
direction: direction/reverse
top level: pBBknock
start: 3033
end: 3089
orientation: inline

BBa_J33204
type: SequenceAnnotation
persistent identity: annotation2478501
display id: annotation2478501
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478501/range2478501/1
component: BBa_J33204

https://synbiohub.org/public/igem/BBa_J107077/annotation2478501/range2478501/1
type: Range
persistent identity: range2478501
display id: range2478501
version: 1
direction: direction/reverse
top level: pBBknock
start: 65
end: 1022
orientation: inline

BBa_K801000
type: ComponentDefinition
persistent identity: BBa K801000
display id: BBa_K801000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM100 yeast shuttle vector based on pYES2
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: A yeast shuttle vector containing the following structural elements: * 2?? ori for replication in ''S. cerevisiae'' * URA3-gene for selection of transformed yeast via Uracil prototrophy * pUC ori for replication in ''E. coli'' * AmpR-gene for selection of transformed ''E. coli'' via Ampicillin resistance * f1 ori * MCS containing the RFC25 pre- and suffix for easy insertion of biobricks * following the MCS: CYC1 yeast transcriptional terminator Note: To use this backbone, a ''S. cerevisiae'' strain with Uracil-auxotrophy (such as strain INVSc1) is required for positive selection of transformants. Successfully transformed yeast cells will become prototrophic for uracil and can be selected on Agar-plates deficient of uracil.
mutable notes: -
mutable provenance: Based on invitrogen's pYES2-plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: URA3
sequence annotation: RFC25 MCS
sequence annotation: Strep-tag II
sequence annotation: 2 micron (??) origin
sequence annotation: T7-Promoter
sequence annotation: CYC1-Terminator
sequence annotation: pUC-origin
sequence annotation: AmpR
sequence: https://synbiohub.org/public/igem/BBa_K801000_sequence/1

URA3
type: SequenceAnnotation
persistent identity: annotation2202325
display id: annotation2202325
version: 1
top level: BBa_K801000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202325/range2202325/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202325/range2202325/1
type: Range
persistent identity: range2202325
display id: range2202325
version: 1
direction: direction/reverse
top level: BBa_K801000
start: 2274
end: 3381
orientation: inline

RFC25 MCS
type: SequenceAnnotation
persistent identity: annotation2202314
display id: annotation2202314
version: 1
top level: BBa_K801000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202314/range2202314/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202314/range2202314/1
type: Range
persistent identity: range2202314
display id: range2202314
version: 1
direction: direction/forward
top level: BBa_K801000
start: 36
end: 134
orientation: inline

Strep-tag II
type: SequenceAnnotation
persistent identity: annotation2202315
display id: annotation2202315
version: 1
top level: BBa_K801000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202315/range2202315/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202315/range2202315/1
type: Range
persistent identity: range2202315
display id: range2202315
version: 1
direction: direction/forward
top level: BBa_K801000
start: 78
end: 101
orientation: inline

2 micron (??) origin
type: SequenceAnnotation
persistent identity: annotation2202326
display id: annotation2202326
version: 1
top level: BBa_K801000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202326/range2202326/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202326/range2202326/1
type: Range
persistent identity: range2202326
display id: range2202326
version: 1
direction: direction/forward
top level: BBa_K801000
start: 3385
end: 4856
orientation: inline

T7-Promoter
type: SequenceAnnotation
persistent identity: annotation2202258
display id: annotation2202258
version: 1
top level: BBa_K801000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202258/range2202258/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202258/range2202258/1
type: Range
persistent identity: range2202258
display id: range2202258
version: 1
direction: direction/forward
top level: BBa_K801000
start: 4
end: 23
orientation: inline

CYC1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202316
display id: annotation2202316
version: 1
top level: BBa_K801000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202316/range2202316/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202316/range2202316/1
type: Range
persistent identity: range2202316
display id: range2202316
version: 1
direction: direction/forward
top level: BBa_K801000
start: 148
end: 396
orientation: inline

pUC-origin
type: SequenceAnnotation
persistent identity: annotation2202317
display id: annotation2202317
version: 1
top level: BBa_K801000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202317/range2202317/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202317/range2202317/1
type: Range
persistent identity: range2202317
display id: range2202317
version: 1
direction: direction/forward
top level: BBa_K801000
start: 578
end: 1251
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2202324
display id: annotation2202324
version: 1
top level: BBa_K801000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202324/range2202324/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202324/range2202324/1
type: Range
persistent identity: range2202324
display id: range2202324
version: 1
direction: direction/reverse
top level: BBa_K801000
start: 1396
end: 2256
orientation: inline

BBa_K1212002
type: ComponentDefinition
persistent identity: BBa K1212002
display id: BBa_K1212002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Golden Gate Compatible pSB3K3
creator: Alex Neckelmann
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1526_
m user id: 0
owner id: 18054
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The low copy backbone, pSB3K3 has a BsaI site at position 2498. Through site-directed mutagenesis, we altered the base pair 2498 G>A, so that it would be Golden Gate assembly-compatible.
mutable notes: The sequence immediately downstream of the p15a origin in this plasmid is an almost exact match to the AmpR cassette (see BBa_P1002). It seems likely that the orignal definition of the p15a origin was generous and could likely be truncated.
mutable provenance: The original pSB3K3 backbone was derived from the parts registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: E. coli his operon terminator
sequence annotation: kanR (rev)
sequence annotation: deletion of two bases
sequence annotation: p15A ORI (rev)
sequence annotation: Fixed BsaI Site
sequence annotation: terminator stem loop
sequence annotation: end of terminator
sequence annotation: VF2
sequence annotation: VR
sequence annotation: Suffix
sequence: https://synbiohub.org/public/igem/BBa_K1212002_sequence/1

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2347333
display id: annotation2347333
version: 1
top level: BBa_K1212002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1212002/annotation2347333/range2347333/1

https://synbiohub.org/public/igem/BBa_K1212002/annotation2347333/range2347333/1
type: Range
persistent identity: range2347333
display id: range2347333
version: 1
direction: direction/forward
top level: BBa_K1212002
start: 22
end: 93
orientation: inline

kanR (rev)
type: SequenceAnnotation
persistent identity: annotation2347359
display id: annotation2347359
version: 1
top level: BBa_K1212002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1212002/annotation2347359/range2347359/1

https://synbiohub.org/public/igem/BBa_K1212002/annotation2347359/range2347359/1
type: Range
persistent identity: range2347359
display id: range2347359
version: 1
direction: direction/reverse
top level: BBa_K1212002
start: 239
end: 1054
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation2347334
display id: annotation2347334
version: 1
top level: BBa_K1212002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1212002/annotation2347334/range2347334/1

https://synbiohub.org/public/igem/BBa_K1212002/annotation2347334/range2347334/1
type: Range
persistent identity: range2347334
display id: range2347334
version: 1
direction: direction/forward
top level: BBa_K1212002
start: 34
end: 35
orientation: inline

p15A ORI (rev)
type: SequenceAnnotation
persistent identity: annotation2347360
display id: annotation2347360
version: 1
top level: BBa_K1212002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1212002/annotation2347360/range2347360/1

https://synbiohub.org/public/igem/BBa_K1212002/annotation2347360/range2347360/1
type: Range
persistent identity: range2347360
display id: range2347360
version: 1
direction: direction/reverse
top level: BBa_K1212002
start: 1386
end: 2210
orientation: inline

Fixed BsaI Site
type: SequenceAnnotation
persistent identity: annotation2347371
display id: annotation2347371
version: 1
top level: BBa_K1212002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1212002/annotation2347371/range2347371/1

https://synbiohub.org/public/igem/BBa_K1212002/annotation2347371/range2347371/1
type: Range
persistent identity: range2347371
display id: range2347371
version: 1
direction: direction/forward
top level: BBa_K1212002
start: 2498
end: 2503
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2347373
display id: annotation2347373
version: 1
top level: BBa_K1212002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1212002/annotation2347373/range2347373/1

https://synbiohub.org/public/igem/BBa_K1212002/annotation2347373/range2347373/1
type: Range
persistent identity: range2347373
display id: range2347373
version: 1
direction: direction/forward
top level: BBa_K1212002
start: 2700
end: 2719
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2347372
display id: annotation2347372
version: 1
top level: BBa_K1212002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1212002/annotation2347372/range2347372/1

https://synbiohub.org/public/igem/BBa_K1212002/annotation2347372/range2347372/1
type: Range
persistent identity: range2347372
display id: range2347372
version: 1
direction: direction/forward
top level: BBa_K1212002
start: 2687
end: 2690
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2347370
display id: annotation2347370
version: 1
top level: BBa_K1212002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1212002/annotation2347370/range2347370/1

https://synbiohub.org/public/igem/BBa_K1212002/annotation2347370/range2347370/1
type: Range
persistent identity: range2347370
display id: range2347370
version: 1
direction: direction/forward
top level: BBa_K1212002
start: 2611
end: 2630
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2347335
display id: annotation2347335
version: 1
top level: BBa_K1212002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1212002/annotation2347335/range2347335/1

https://synbiohub.org/public/igem/BBa_K1212002/annotation2347335/range2347335/1
type: Range
persistent identity: range2347335
display id: range2347335
version: 1
direction: direction/reverse
top level: BBa_K1212002
start: 157
end: 176
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2347332
display id: annotation2347332
version: 1
top level: BBa_K1212002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1212002/annotation2347332/range2347332/1

https://synbiohub.org/public/igem/BBa_K1212002/annotation2347332/range2347332/1
type: Range
persistent identity: range2347332
display id: range2347332
version: 1
direction: direction/forward
top level: BBa_K1212002
start: 30
end: 64
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2347330
display id: annotation2347330
version: 1
top level: BBa_K1212002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1212002/annotation2347330/range2347330/1

https://synbiohub.org/public/igem/BBa_K1212002/annotation2347330/range2347330/1
type: Range
persistent identity: range2347330
display id: range2347330
version: 1
direction: direction/forward
top level: BBa_K1212002
start: 1
end: 21
orientation: inline

BBa_K209499
type: ComponentDefinition
persistent identity: BBa K209499
display id: BBa_K209499
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pEF5-FRT-V5-DEST
creator: Benjamin Rhau
created: 2009-10-18T11:00:00Z
modified: 2015-06-15T02:55:50Z
discontinued: true
dominant: false
experience: experience/None
group u list: _312_
m user id: 4206
owner id: 3698
owning group id: 9
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable description: Commercial plasmid used in the construction of other composite parts.
mutable notes: Not a designed plasmid
mutable provenance: Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K209499_sequence/1

BBa_K864011
type: ComponentDefinition
persistent identity: BBa K864011
display id: BBa_K864011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864011_sequence/1

BBa_J72112
type: ComponentDefinition
persistent identity: BBa J72112
display id: BBa_J72112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpecR split R6K vector
creator: Josh Kittleson
created: 2011-11-09T12:00:00Z
modified: 2015-05-08T01:08:27Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Requires pir gene provided in trans to replicate.
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72112_sequence/1

BBa_K270002
type: ComponentDefinition
persistent identity: BBa K270002
display id: BBa_K270002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leucine Landing Pad
creator: Ann Lesnefsky
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:44Z
discontinued: false
dominant: false
group u list: _368_
m user id: 0
owner id: 4465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Used to put parts on the chromosome.
mutable notes: This was a biobrick compatable vector available in the lab to place our parts on.
mutable provenance: constructed by former Ninfa lab member, Don Eun Chang
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Prefix
sequence annotation: Suffix
sequence: https://synbiohub.org/public/igem/BBa_K270002_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation2054691
display id: annotation2054691
version: 1
top level: BBa_K270002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K270002/annotation2054691/range2054691/1

https://synbiohub.org/public/igem/BBa_K270002/annotation2054691/range2054691/1
type: Range
persistent identity: range2054691
display id: range2054691
version: 1
direction: direction/forward
top level: BBa_K270002
start: 5821
end: 5842
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2054690
display id: annotation2054690
version: 1
top level: BBa_K270002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K270002/annotation2054690/range2054690/1

https://synbiohub.org/public/igem/BBa_K270002/annotation2054690/range2054690/1
type: Range
persistent identity: range2054690
display id: range2054690
version: 1
direction: direction/forward
top level: BBa_K270002
start: 1
end: 21
orientation: inline

BBa_K2100015
type: ComponentDefinition
persistent identity: BBa K2100015
display id: BBa_K2100015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pDEST_mCherry_GG
creator: Kathleen Brandes
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T08:40:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2568_
m user id: 32435
owner id: 31606
owning group id: 9
status: status/Planning
bookmark: false
mutable description: DESCRIPTION HERE!
mutable notes: DESIGN DECISIONS HERE!
mutable provenance: This is synthesized from cloning.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K2100015_sequence/1

BBa_K1438031
type: ComponentDefinition
persistent identity: BBa K1438031
display id: BBa_K1438031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pQE_80L
creator: Johann Bauerfeind
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:25Z
discontinued: false
dominant: false
group u list: _1816_
m user id: 0
owner id: 19714
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pQE_80L Expression Plasmi
mutable notes: pQE_80L Expression Plasmi
mutable provenance: pQE_80L Expression Plasmi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1438031_sequence/1

pSB1A30
type: ComponentDefinition
persistent identity: BBa K1362091
display id: BBa_K1362091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A30: High copy BioBrick cloning/expression backbone carrying Amp resitance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VF2
sequence annotation: suffix
sequence annotation: rep(pMB1) (rev)
sequence annotation: prefix
sequence annotation: end of terminator
sequence annotation: E. coli his operon terminator
sequence annotation: terminator stem loop
sequence annotation: lacO reg
sequence annotation: VR
sequence annotation: T7 promotor
sequence annotation: ampR (rev)
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_K1362091_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation2404608
display id: annotation2404608
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1
type: Range
persistent identity: range2404608
display id: range2404608
version: 1
direction: direction/forward
top level: pSB1A30
start: 2018
end: 2037
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2404600
display id: annotation2404600
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1
type: Range
persistent identity: range2404600
display id: range2404600
version: 1
direction: direction/forward
top level: pSB1A30
start: 1
end: 21
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2404605
display id: annotation2404605
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1
type: Range
persistent identity: range2404605
display id: range2404605
version: 1
direction: direction/reverse
top level: pSB1A30
start: 261
end: 875
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2404632
display id: annotation2404632
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1
type: Range
persistent identity: range2404632
display id: range2404632
version: 1
direction: direction/forward
top level: pSB1A30
start: 2185
end: 2206
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2404609
display id: annotation2404609
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1
type: Range
persistent identity: range2404609
display id: range2404609
version: 1
direction: direction/reverse
top level: pSB1A30
start: 2094
end: 2097
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2404601
display id: annotation2404601
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1
type: Range
persistent identity: range2404601
display id: range2404601
version: 1
direction: direction/forward
top level: pSB1A30
start: 22
end: 93
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404603
display id: annotation2404603
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1
type: Range
persistent identity: range2404603
display id: range2404603
version: 1
direction: direction/forward
top level: pSB1A30
start: 30
end: 64
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404610
display id: annotation2404610
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1
type: Range
persistent identity: range2404610
display id: range2404610
version: 1
direction: direction/forward
top level: pSB1A30
start: 2107
end: 2126
orientation: inline

lacO reg
type: SequenceAnnotation
persistent identity: annotation2404631
display id: annotation2404631
version: 1
top level: pSB1A30
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1
type: Range
persistent identity: range2404631
display id: range2404631
version: 1
direction: direction/forward
top level: pSB1A30
start: 2157
end: 2184
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2404602
display id: annotation2404602
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1
type: Range
persistent identity: range2404602
display id: range2404602
version: 1
direction: direction/reverse
top level: pSB1A30
start: 157
end: 176
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2404611
display id: annotation2404611
version: 1
top level: pSB1A30
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1
type: Range
persistent identity: range2404611
display id: range2404611
version: 1
direction: direction/forward
top level: pSB1A30
start: 2139
end: 2157
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation2404607
display id: annotation2404607
version: 1
top level: pSB1A30
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1
type: Range
persistent identity: range2404607
display id: range2404607
version: 1
direction: direction/reverse
top level: pSB1A30
start: 1035
end: 1895
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2404606
display id: annotation2404606
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1
type: Range
persistent identity: range2404606
display id: range2404606
version: 1
direction: direction/forward
top level: pSB1A30
start: 276
end: 276
orientation: inline

BBa_K249004
type: ComponentDefinition
persistent identity: BBa K249004
display id: BBa_K249004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: N-terminal Arginine fusion vector
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pSB1A3 with an N-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly. Adds 10 Arginine residues to the N-terminus of a Protein.
mutable notes: Standard
mutable provenance: Synthesized Tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K249004_sequence/1

BBa_K209473
type: ComponentDefinition
persistent identity: BBa K209473
display id: BBa_K209473
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcDNA3.1(+) with AarI A-D sites in MCS
creator: Katja Kolar
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:11:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _312_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K209473_sequence/1

pSB4K50
type: ComponentDefinition
persistent identity: BBa K1362097
display id: BBa_K1362097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick expression backbone carrying Kan resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_K1362463
component: BBa_G00100
component: BBa_B0042
component: BBa_G00102
component: BBa_I50041
component: BBa_G00001
component: BBa_B0062
component: BBa_G0001
component: BBa_B0043
component: BBa_B0055
component: BBa_B0054
component: BBa_G00000
component: BBa_G0005
component: BBa_P1007
component: BBa_G0006
component: BBa_B0053
component: BBa_B0044
sequence annotation: BBa_G00001
sequence annotation: BBa_G0001
sequence annotation: BBa_P1007
sequence annotation: BBa_G00100
sequence annotation: BBa_B0055
sequence annotation: BBa_I50041
sequence annotation: BBa_B0053
sequence annotation: BBa_G00102
sequence annotation: BBa_B0062
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_G0006
sequence annotation: BBa_G0005
sequence annotation: BBa_B0043
sequence annotation: BBa_K1362463
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_K1362097_sequence/1

BBa_K1362463
type: Component
persistent identity: component2410012
display id: component2410012
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_G00100
type: Component
persistent identity: component2409993
display id: component2409993
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0042
type: Component
persistent identity: component2410003
display id: component2410003
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00102
type: Component
persistent identity: component2409954
display id: component2409954
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_I50041
type: Component
persistent identity: component2409988
display id: component2409988
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50041/1

BBa_G00001
type: Component
persistent identity: component2409937
display id: component2409937
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0062
type: Component
persistent identity: component2409956
display id: component2409956
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0001
type: Component
persistent identity: component2409962
display id: component2409962
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0001/1

BBa_B0043
type: Component
persistent identity: component2410008
display id: component2410008
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0055
type: Component
persistent identity: component2409996
display id: component2409996
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0054
type: Component
persistent identity: component2409952
display id: component2409952
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00000
type: Component
persistent identity: component2410017
display id: component2410017
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G0005
type: Component
persistent identity: component2409957
display id: component2409957
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0005/1

BBa_B0042
type: Component
persistent identity: component2409949
display id: component2409949
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1007
type: Component
persistent identity: component2409961
display id: component2409961
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1007/1

BBa_G0006
type: Component
persistent identity: component2409989
display id: component2409989
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0006/1

BBa_B0053
type: Component
persistent identity: component2409991
display id: component2409991
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0044
type: Component
persistent identity: component2409942
display id: component2409942
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409937
display id: annotation2409937
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409937/range2409937/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409937/range2409937/1
type: Range
persistent identity: range2409937
display id: range2409937
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1
end: 21
orientation: inline

BBa_G0001
type: SequenceAnnotation
persistent identity: annotation2409962
display id: annotation2409962
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409962/range2409962/1
component: BBa_G0001

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409962/range2409962/1
type: Range
persistent identity: range2409962
display id: range2409962
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1151
end: 1158
orientation: inline

BBa_P1007
type: SequenceAnnotation
persistent identity: annotation2409961
display id: annotation2409961
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409961/range2409961/1
component: BBa_P1007

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409961/range2409961/1
type: Range
persistent identity: range2409961
display id: range2409961
version: 1
direction: direction/reverse
top level: pSB4K50
start: 184
end: 1150
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409993
display id: annotation2409993
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409993/range2409993/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409993/range2409993/1
type: Range
persistent identity: range2409993
display id: range2409993
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3275
end: 3294
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409996
display id: annotation2409996
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409996/range2409996/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409996/range2409996/1
type: Range
persistent identity: range2409996
display id: range2409996
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3295
end: 3372
orientation: inline

BBa_I50041
type: SequenceAnnotation
persistent identity: annotation2409988
display id: annotation2409988
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409988/range2409988/1
component: BBa_I50041

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409988/range2409988/1
type: Range
persistent identity: range2409988
display id: range2409988
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1159
end: 3195
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409991
display id: annotation2409991
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409991/range2409991/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409991/range2409991/1
type: Range
persistent identity: range2409991
display id: range2409991
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3203
end: 3274
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409954
display id: annotation2409954
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409954/range2409954/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409954/range2409954/1
type: Range
persistent identity: range2409954
display id: range2409954
version: 1
direction: direction/reverse
top level: pSB4K50
start: 116
end: 135
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409956
display id: annotation2409956
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409956/range2409956/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409956/range2409956/1
type: Range
persistent identity: range2409956
display id: range2409956
version: 1
direction: direction/reverse
top level: pSB4K50
start: 136
end: 176
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409942
display id: annotation2409942
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409942/range2409942/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409942/range2409942/1
type: Range
persistent identity: range2409942
display id: range2409942
version: 1
direction: direction/reverse
top level: pSB4K50
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409949
display id: annotation2409949
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409949/range2409949/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409949/range2409949/1
type: Range
persistent identity: range2409949
display id: range2409949
version: 1
direction: direction/reverse
top level: pSB4K50
start: 35
end: 46
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409952
display id: annotation2409952
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409952/range2409952/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409952/range2409952/1
type: Range
persistent identity: range2409952
display id: range2409952
version: 1
direction: direction/reverse
top level: pSB4K50
start: 47
end: 115
orientation: inline

BBa_G0006
type: SequenceAnnotation
persistent identity: annotation2409989
display id: annotation2409989
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409989/range2409989/1
component: BBa_G0006

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409989/range2409989/1
type: Range
persistent identity: range2409989
display id: range2409989
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3196
end: 3202
orientation: inline

BBa_G0005
type: SequenceAnnotation
persistent identity: annotation2409957
display id: annotation2409957
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409957/range2409957/1
component: BBa_G0005

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409957/range2409957/1
type: Range
persistent identity: range2409957
display id: range2409957
version: 1
direction: direction/reverse
top level: pSB4K50
start: 177
end: 183
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2410008
display id: annotation2410008
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410008/range2410008/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410008/range2410008/1
type: Range
persistent identity: range2410008
display id: range2410008
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3385
end: 3397
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2410012
display id: annotation2410012
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410012/range2410012/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410012/range2410012/1
type: Range
persistent identity: range2410012
display id: range2410012
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3398
end: 3448
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2410003
display id: annotation2410003
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410003/range2410003/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410003/range2410003/1
type: Range
persistent identity: range2410003
display id: range2410003
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3373
end: 3384
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2410017
display id: annotation2410017
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410017/range2410017/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410017/range2410017/1
type: Range
persistent identity: range2410017
display id: range2410017
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3449
end: 3470
orientation: inline

pFun
type: ComponentDefinition
persistent identity: BBa K678046
display id: BBa_K678046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid-Fun for Plug
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plasmid for Plug'n'Play. Contains an Ampicillin resistance marker.
mutable notes: Compatible with the Plug'n'Play assembly standard.
mutable provenance: p71
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Linker 6
sequence annotation: pFun
sequence: https://synbiohub.org/public/igem/BBa_K678046_sequence/1

Linker 6
type: SequenceAnnotation
persistent identity: annotation2127423
display id: annotation2127423
version: 1
top level: pFun
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678046/annotation2127423/range2127423/1

https://synbiohub.org/public/igem/BBa_K678046/annotation2127423/range2127423/1
type: Range
persistent identity: range2127423
display id: range2127423
version: 1
direction: direction/forward
top level: pFun
start: 1
end: 8
orientation: inline

pFun
type: SequenceAnnotation
persistent identity: annotation2127424
display id: annotation2127424
version: 1
top level: pFun
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678046/annotation2127424/range2127424/1

https://synbiohub.org/public/igem/BBa_K678046/annotation2127424/range2127424/1
type: Range
persistent identity: range2127424
display id: range2127424
version: 1
direction: direction/forward
top level: pFun
start: 9
end: 2878
orientation: inline

BBa_J119061
type: ComponentDefinition
persistent identity: BBa J119061
display id: BBa_J119061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A2-BR
creator: Todd Eckdahl
created: 2012-01-27T12:00:00Z
modified: 2015-08-31T04:08:26Z
discontinued: false
dominant: false
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid pSB1A2 with BsaI site removed from Ampicillin resistance gene. For use in Golden Gate Assembly with BsaI.
mutable notes: Original amino acid sequence of Amplicillin gene retained.
mutable provenance: PCR mutagenesis of pSB1A2.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Terminator
sequence annotation: c -> a (BsaI site removed)
sequence annotation: VR
sequence annotation: Prefix
sequence annotation: Ampicillin resistance gene (rev)
sequence annotation: Suffix
sequence annotation: VF2
sequence annotation: rep (pMB1) (rev)
sequence: https://synbiohub.org/public/igem/BBa_J119061_sequence/1

Terminator
type: SequenceAnnotation
persistent identity: annotation2169887
display id: annotation2169887
version: 1
top level: BBa_J119061
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J119061/annotation2169887/range2169887/1

https://synbiohub.org/public/igem/BBa_J119061/annotation2169887/range2169887/1
type: Range
persistent identity: range2169887
display id: range2169887
version: 1
direction: direction/forward
top level: BBa_J119061
start: 2029
end: 2048
orientation: inline

c -> a (BsaI site removed)
type: SequenceAnnotation
persistent identity: annotation2169888
display id: annotation2169888
version: 1
top level: BBa_J119061
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J119061/annotation2169888/range2169888/1

https://synbiohub.org/public/igem/BBa_J119061/annotation2169888/range2169888/1
type: Range
persistent identity: range2169888
display id: range2169888
version: 1
direction: direction/forward
top level: BBa_J119061
start: 1101
end: 1101
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2169883
display id: annotation2169883
version: 1
top level: BBa_J119061
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119061/annotation2169883/range2169883/1

https://synbiohub.org/public/igem/BBa_J119061/annotation2169883/range2169883/1
type: Range
persistent identity: range2169883
display id: range2169883
version: 1
direction: direction/reverse
top level: BBa_J119061
start: 79
end: 98
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2169882
display id: annotation2169882
version: 1
top level: BBa_J119061
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119061/annotation2169882/range2169882/1

https://synbiohub.org/public/igem/BBa_J119061/annotation2169882/range2169882/1
type: Range
persistent identity: range2169882
display id: range2169882
version: 1
direction: direction/forward
top level: BBa_J119061
start: 2058
end: 2079
orientation: inline

Ampicillin resistance gene (rev)
type: SequenceAnnotation
persistent identity: annotation2169886
display id: annotation2169886
version: 1
top level: BBa_J119061
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J119061/annotation2169886/range2169886/1

https://synbiohub.org/public/igem/BBa_J119061/annotation2169886/range2169886/1
type: Range
persistent identity: range2169886
display id: range2169886
version: 1
direction: direction/reverse
top level: BBa_J119061
start: 957
end: 1817
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2169881
display id: annotation2169881
version: 1
top level: BBa_J119061
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119061/annotation2169881/range2169881/1

https://synbiohub.org/public/igem/BBa_J119061/annotation2169881/range2169881/1
type: Range
persistent identity: range2169881
display id: range2169881
version: 1
direction: direction/forward
top level: BBa_J119061
start: 1
end: 21
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2169884
display id: annotation2169884
version: 1
top level: BBa_J119061
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119061/annotation2169884/range2169884/1

https://synbiohub.org/public/igem/BBa_J119061/annotation2169884/range2169884/1
type: Range
persistent identity: range2169884
display id: range2169884
version: 1
direction: direction/forward
top level: BBa_J119061
start: 1940
end: 1959
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2169885
display id: annotation2169885
version: 1
top level: BBa_J119061
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J119061/annotation2169885/range2169885/1

https://synbiohub.org/public/igem/BBa_J119061/annotation2169885/range2169885/1
type: Range
persistent identity: range2169885
display id: range2169885
version: 1
direction: direction/reverse
top level: BBa_J119061
start: 183
end: 797
orientation: inline

pSB4C1
type: ComponentDefinition
persistent identity: pSB4C1
display id: pSB4C1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCK01 converted into BioBrick vector (formerly BBa_I739202)
creator: Stefan Luzi
created: 2007-10-23T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick prefix
sequence annotation: C->T mutation to eliminate SpeI site
sequence annotation: pSC101 replication initiator protein
sequence annotation: chloramphenicol acetyltransferase
sequence annotation: BioBrick suffix
sequence annotation: Mutagenesis primer
sequence: https://synbiohub.org/public/igem/pSB4C1_sequence/1

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969636
display id: annotation1969636
version: 1
top level: pSB4C1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969636/range1969636/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969636/range1969636/1
type: Range
persistent identity: range1969636
display id: range1969636
version: 1
direction: direction/forward
top level: pSB4C1
start: 3134
end: 3155
orientation: inline

C->T mutation to eliminate SpeI site
type: SequenceAnnotation
persistent identity: annotation1969634
display id: annotation1969634
version: 1
top level: pSB4C1
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969634/range1969634/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969634/range1969634/1
type: Range
persistent identity: range1969634
display id: range1969634
version: 1
direction: direction/forward
top level: pSB4C1
start: 1508
end: 1508
orientation: inline

pSC101 replication initiator protein
type: SequenceAnnotation
persistent identity: annotation1969632
display id: annotation1969632
version: 1
top level: pSB4C1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969632/range1969632/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969632/range1969632/1
type: Range
persistent identity: range1969632
display id: range1969632
version: 1
direction: direction/reverse
top level: pSB4C1
start: 490
end: 1440
orientation: inline

chloramphenicol acetyltransferase
type: SequenceAnnotation
persistent identity: annotation1969633
display id: annotation1969633
version: 1
top level: pSB4C1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969633/range1969633/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969633/range1969633/1
type: Range
persistent identity: range1969633
display id: range1969633
version: 1
direction: direction/forward
top level: pSB4C1
start: 2311
end: 2970
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969631
display id: annotation1969631
version: 1
top level: pSB4C1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969631/range1969631/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969631/range1969631/1
type: Range
persistent identity: range1969631
display id: range1969631
version: 1
direction: direction/forward
top level: pSB4C1
start: 1
end: 21
orientation: inline

Mutagenesis primer
type: SequenceAnnotation
persistent identity: annotation1969635
display id: annotation1969635
version: 1
top level: pSB4C1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969635/range1969635/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969635/range1969635/1
type: Range
persistent identity: range1969635
display id: range1969635
version: 1
direction: direction/reverse
top level: pSB4C1
start: 1491
end: 1529
orientation: inline

BBa_J72113
type: ComponentDefinition
persistent identity: BBa J72113
display id: BBa_J72113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CamR p15A vector
creator: Josh Kittleson
created: 2011-11-09T12:00:00Z
modified: 2015-05-08T01:08:27Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: na
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72113_sequence/1

BBa_K1321301
type: ComponentDefinition
persistent identity: BBa K1321301
display id: BBa_K1321301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSEVA321-Bb
creator: Michael Florea
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1696_
m user id: 0
owner id: 20779
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is pSEVA321 converted into a biobrick compatible format by substituting the original multiple cloning site for biobrick prefix and suffix. pSEVA321 is a broad host range plasmid, capable of replication in E.coli and several other organisms. It is capable of replication in the cellulose producing bacterium Gluconacetobacter xylinum. We have used it as our standard backbone for G.xylinum genetic engineering.
mutable notes: None
mutable provenance: The original pSEVA331 backbone comes from the SEVA (Standard European Vector Architecture) collection.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: OriT
sequence annotation: Biobrick prefix
sequence annotation: Replication protein TrfA
sequence annotation: Chloramphenicol acetyltransferase
sequence annotation: Biobrick suffix
sequence: https://synbiohub.org/public/igem/BBa_K1321301_sequence/1

OriT
type: SequenceAnnotation
persistent identity: annotation2408626
display id: annotation2408626
version: 1
top level: BBa_K1321301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321301/annotation2408626/range2408626/1

https://synbiohub.org/public/igem/BBa_K1321301/annotation2408626/range2408626/1
type: Range
persistent identity: range2408626
display id: range2408626
version: 1
direction: direction/forward
top level: BBa_K1321301
start: 954
end: 1189
orientation: inline

Biobrick prefix
type: SequenceAnnotation
persistent identity: annotation2408624
display id: annotation2408624
version: 1
top level: BBa_K1321301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321301/annotation2408624/range2408624/1

https://synbiohub.org/public/igem/BBa_K1321301/annotation2408624/range2408624/1
type: Range
persistent identity: range2408624
display id: range2408624
version: 1
direction: direction/forward
top level: BBa_K1321301
start: 3572
end: 3593
orientation: inline

Replication protein TrfA
type: SequenceAnnotation
persistent identity: annotation2408627
display id: annotation2408627
version: 1
top level: BBa_K1321301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321301/annotation2408627/range2408627/1

https://synbiohub.org/public/igem/BBa_K1321301/annotation2408627/range2408627/1
type: Range
persistent identity: range2408627
display id: range2408627
version: 1
direction: direction/reverse
top level: BBa_K1321301
start: 1248
end: 2396
orientation: inline

Chloramphenicol acetyltransferase
type: SequenceAnnotation
persistent identity: annotation2408625
display id: annotation2408625
version: 1
top level: BBa_K1321301
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1321301/annotation2408625/range2408625/1

https://synbiohub.org/public/igem/BBa_K1321301/annotation2408625/range2408625/1
type: Range
persistent identity: range2408625
display id: range2408625
version: 1
direction: direction/forward
top level: BBa_K1321301
start: 271
end: 930
orientation: inline

Biobrick suffix
type: SequenceAnnotation
persistent identity: annotation2408623
display id: annotation2408623
version: 1
top level: BBa_K1321301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321301/annotation2408623/range2408623/1

https://synbiohub.org/public/igem/BBa_K1321301/annotation2408623/range2408623/1
type: Range
persistent identity: range2408623
display id: range2408623
version: 1
direction: direction/forward
top level: BBa_K1321301
start: 1
end: 21
orientation: inline

pSB3K3
type: ComponentDefinition
persistent identity: pSB3K3
display id: pSB3K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2004-06-28T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3K3-1 is a medium copy plasmid with kanamycin resistance. It has a p15A pMR101-derived replication origin, copy number 20-30. (Lutz and Bujard, 1997) pSB3K3-1 has a terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into the vectorl. A second terminator (E.coli His operon-derived) is downstream of the MCS, again insulating the vector from transcription reading out of the MCS. Ideally, future versions of standard biobrick vectors would have terminators bracketing the MCS that were 100% efficient in terminating transcription both into and out of the MCS region.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: kanR (rev)
sequence annotation: E. coli his operon terminator
sequence annotation: Prefix
sequence annotation: VR
sequence annotation: deletion of two bases
sequence annotation: terminator stem loop
sequence annotation: VF2
sequence annotation: p15A ORI (rev)
sequence annotation: end of terminator
sequence annotation: Suffix
sequence: https://synbiohub.org/public/igem/pSB3K3_sequence/1

kanR (rev)
type: SequenceAnnotation
persistent identity: annotation1023045
display id: annotation1023045
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1
type: Range
persistent identity: range1023045
display id: range1023045
version: 1
direction: direction/reverse
top level: pSB3K3
start: 239
end: 1054
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824021
display id: annotation1824021
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1
type: Range
persistent identity: range1824021
display id: range1824021
version: 1
direction: direction/forward
top level: pSB3K3
start: 22
end: 93
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1023043
display id: annotation1023043
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1
type: Range
persistent identity: range1023043
display id: range1023043
version: 1
direction: direction/forward
top level: pSB3K3
start: 2729
end: 2750
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782476
display id: annotation1782476
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1
type: Range
persistent identity: range1782476
display id: range1782476
version: 1
direction: direction/reverse
top level: pSB3K3
start: 157
end: 176
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823788
display id: annotation1823788
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1

https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1
type: Range
persistent identity: range1823788
display id: range1823788
version: 1
direction: direction/forward
top level: pSB3K3
start: 34
end: 35
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824022
display id: annotation1824022
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1
type: Range
persistent identity: range1824022
display id: range1824022
version: 1
direction: direction/forward
top level: pSB3K3
start: 30
end: 64
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782459
display id: annotation1782459
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1
type: Range
persistent identity: range1782459
display id: range1782459
version: 1
direction: direction/forward
top level: pSB3K3
start: 2611
end: 2630
orientation: inline

p15A ORI (rev)
type: SequenceAnnotation
persistent identity: annotation1023046
display id: annotation1023046
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1
type: Range
persistent identity: range1023046
display id: range1023046
version: 1
direction: direction/reverse
top level: pSB3K3
start: 1386
end: 2210
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824024
display id: annotation1824024
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1
type: Range
persistent identity: range1824024
display id: range1824024
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2687
end: 2690
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1023044
display id: annotation1023044
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1
type: Range
persistent identity: range1023044
display id: range1023044
version: 1
direction: direction/forward
top level: pSB3K3
start: 1
end: 21
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824023
display id: annotation1824023
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1
type: Range
persistent identity: range1824023
display id: range1824023
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2700
end: 2719
orientation: inline

BBa_K1321307
type: ComponentDefinition
persistent identity: BBa K1321307
display id: BBa_K1321307
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSEVA351-Bb
creator: Michael Florea
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1696_
m user id: 0
owner id: 20779
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: X
mutable notes: Z
mutable provenance: Y
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Biobrick prefix
sequence annotation: OriT
sequence annotation: RepB
sequence annotation: PlacUV5
sequence annotation: RepC,A (replication proteins)
sequence annotation: NgoMIV RE site
sequence annotation: Chloramphenicol acetyltransferase
sequence annotation: Biobrick suffix
sequence: https://synbiohub.org/public/igem/BBa_K1321307_sequence/1

Biobrick prefix
type: SequenceAnnotation
persistent identity: annotation2425182
display id: annotation2425182
version: 1
top level: BBa_K1321307
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321307/annotation2425182/range2425182/1

https://synbiohub.org/public/igem/BBa_K1321307/annotation2425182/range2425182/1
type: Range
persistent identity: range2425182
display id: range2425182
version: 1
direction: direction/forward
top level: BBa_K1321307
start: 5024
end: 5045
orientation: inline

OriT
type: SequenceAnnotation
persistent identity: annotation2425129
display id: annotation2425129
version: 1
top level: BBa_K1321307
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321307/annotation2425129/range2425129/1

https://synbiohub.org/public/igem/BBa_K1321307/annotation2425129/range2425129/1
type: Range
persistent identity: range2425129
display id: range2425129
version: 1
direction: direction/forward
top level: BBa_K1321307
start: 954
end: 1189
orientation: inline

RepB
type: SequenceAnnotation
persistent identity: annotation2425165
display id: annotation2425165
version: 1
top level: BBa_K1321307
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321307/annotation2425165/range2425165/1

https://synbiohub.org/public/igem/BBa_K1321307/annotation2425165/range2425165/1
type: Range
persistent identity: range2425165
display id: range2425165
version: 1
direction: direction/forward
top level: BBa_K1321307
start: 3386
end: 4357
orientation: inline

PlacUV5
type: SequenceAnnotation
persistent identity: annotation2425166
display id: annotation2425166
version: 1
top level: BBa_K1321307
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321307/annotation2425166/range2425166/1

https://synbiohub.org/public/igem/BBa_K1321307/annotation2425166/range2425166/1
type: Range
persistent identity: range2425166
display id: range2425166
version: 1
direction: direction/forward
top level: BBa_K1321307
start: 4382
end: 4411
orientation: inline

RepC,A (replication proteins)
type: SequenceAnnotation
persistent identity: annotation2425142
display id: annotation2425142
version: 1
top level: BBa_K1321307
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321307/annotation2425142/range2425142/1

https://synbiohub.org/public/igem/BBa_K1321307/annotation2425142/range2425142/1
type: Range
persistent identity: range2425142
display id: range2425142
version: 1
direction: direction/forward
top level: BBa_K1321307
start: 1198
end: 2875
orientation: inline

NgoMIV RE site
type: SequenceAnnotation
persistent identity: annotation2425141
display id: annotation2425141
version: 1
top level: BBa_K1321307
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321307/annotation2425141/range2425141/1

https://synbiohub.org/public/igem/BBa_K1321307/annotation2425141/range2425141/1
type: Range
persistent identity: range2425141
display id: range2425141
version: 1
direction: direction/forward
top level: BBa_K1321307
start: 1191
end: 1196
orientation: inline

Chloramphenicol acetyltransferase
type: SequenceAnnotation
persistent identity: annotation2425123
display id: annotation2425123
version: 1
top level: BBa_K1321307
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321307/annotation2425123/range2425123/1

https://synbiohub.org/public/igem/BBa_K1321307/annotation2425123/range2425123/1
type: Range
persistent identity: range2425123
display id: range2425123
version: 1
direction: direction/forward
top level: BBa_K1321307
start: 271
end: 931
orientation: inline

Biobrick suffix
type: SequenceAnnotation
persistent identity: annotation2425122
display id: annotation2425122
version: 1
top level: BBa_K1321307
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321307/annotation2425122/range2425122/1

https://synbiohub.org/public/igem/BBa_K1321307/annotation2425122/range2425122/1
type: Range
persistent identity: range2425122
display id: range2425122
version: 1
direction: direction/forward
top level: BBa_K1321307
start: 1
end: 21
orientation: inline

pSBBs0K
type: ComponentDefinition
persistent identity: BBa K823026
display id: BBa_K823026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs0K-Pspac (replicative Bacillus subtilis expression vector; IPTG inducible
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has a ampicillin resistance for cloning in E.coli and kanamycin resistance for selection in B. subtilis. The multiple cloning site is downstream of a Pspac promoter, that is inducible with IPTG. (Isopropyl-β-D-thiogalactopyranosid). This BioBrick is part of the LMU 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli. It can be used as an inducible expression vector in B. subtilis.
mutable provenance: This part is an igem compatible version of the Bacillus expression vector pDG148
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Pspac
sequence annotation: lacI
sequence annotation: repU
sequence annotation: kan
sequence annotation: bla
sequence annotation: ble
sequence annotation: lacY
sequence: https://synbiohub.org/public/igem/BBa_K823026_sequence/1

Pspac
type: SequenceAnnotation
persistent identity: annotation2180262
display id: annotation2180262
version: 1
top level: pSBBs0K
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180262/range2180262/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180262/range2180262/1
type: Range
persistent identity: range2180262
display id: range2180262
version: 1
direction: direction/forward
top level: pSBBs0K
start: 8193
end: 8258
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2180256
display id: annotation2180256
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180256/range2180256/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180256/range2180256/1
type: Range
persistent identity: range2180256
display id: range2180256
version: 1
direction: direction/forward
top level: pSBBs0K
start: 271
end: 1353
orientation: inline

repU
type: SequenceAnnotation
persistent identity: annotation2180258
display id: annotation2180258
version: 1
top level: pSBBs0K
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180258/range2180258/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180258/range2180258/1
type: Range
persistent identity: range2180258
display id: range2180258
version: 1
direction: direction/forward
top level: pSBBs0K
start: 4093
end: 5097
orientation: inline

kan
type: SequenceAnnotation
persistent identity: annotation2180259
display id: annotation2180259
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180259/range2180259/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180259/range2180259/1
type: Range
persistent identity: range2180259
display id: range2180259
version: 1
direction: direction/forward
top level: pSBBs0K
start: 5257
end: 6027
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180257
display id: annotation2180257
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180257/range2180257/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180257/range2180257/1
type: Range
persistent identity: range2180257
display id: range2180257
version: 1
direction: direction/reverse
top level: pSBBs0K
start: 2609
end: 3469
orientation: inline

ble
type: SequenceAnnotation
persistent identity: annotation2180260
display id: annotation2180260
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180260/range2180260/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180260/range2180260/1
type: Range
persistent identity: range2180260
display id: range2180260
version: 1
direction: direction/forward
top level: pSBBs0K
start: 6250
end: 6648
orientation: inline

lacY
type: SequenceAnnotation
persistent identity: annotation2180261
display id: annotation2180261
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180261/range2180261/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180261/range2180261/1
type: Range
persistent identity: range2180261
display id: range2180261
version: 1
direction: direction/reverse
top level: pSBBs0K
start: 6906
end: 7871
orientation: inline

BBa_K260002
type: ComponentDefinition
persistent identity: BBa K260002
display id: BBa_K260002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTetFlp. [anhydrotetracycline] -> FLP(F70L)
creator: Kaj Bernhardt
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _356_
m user id: 0
owner id: 1072
owning group id: 57
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This plasmid is based on pSC101 and contains TetR as well as the F70L variant of FLP recombinase under tet-promoter control. Therefore anhydrotetracycline can be used to induce FLP(F70L) expression. The pSC101 origin of replication is temperature sensitive due to a mutation of repA. Permisive temperature is 30 degC. 37 degC should be avoided as the plasmid can not replicate.
mutable notes: dhfr had to be deleted as the same gene for trimpethoprim resistance was going to be used elsewhere in the same cell.
mutable provenance: This plasmid comes from Sarov M, Schneider S, Pozniakovski A, Roguev A, Ernst S, Zhang Y, Hyman AA, Stewart AF. A recombineering pipeline for functional genomics applied to Caenorhabditis elegans. Nat Methods. 2006 Oct;3(10):839-44. However, it was modified by deletion of dhfr gene and RedABGRecA operon through insertion of a kanamycin resistance cassette.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: repA
sequence annotation: pSC101 origin
sequence annotation: HygR
sequence annotation: KanR
sequence annotation: TetR
sequence annotation: T4_32 terminator
sequence annotation: FLP(F70L)
sequence annotation: prefix
sequence annotation: tet-promoter
sequence annotation: suffix
sequence: https://synbiohub.org/public/igem/BBa_K260002_sequence/1

repA
type: SequenceAnnotation
persistent identity: annotation2048711
display id: annotation2048711
version: 1
top level: BBa_K260002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260002/annotation2048711/range2048711/1

https://synbiohub.org/public/igem/BBa_K260002/annotation2048711/range2048711/1
type: Range
persistent identity: range2048711
display id: range2048711
version: 1
direction: direction/forward
top level: BBa_K260002
start: 820
end: 1770
orientation: inline

pSC101 origin
type: SequenceAnnotation
persistent identity: annotation2048710
display id: annotation2048710
version: 1
top level: BBa_K260002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260002/annotation2048710/range2048710/1

https://synbiohub.org/public/igem/BBa_K260002/annotation2048710/range2048710/1
type: Range
persistent identity: range2048710
display id: range2048710
version: 1
direction: direction/forward
top level: BBa_K260002
start: 75
end: 436
orientation: inline

HygR
type: SequenceAnnotation
persistent identity: annotation2048713
display id: annotation2048713
version: 1
top level: BBa_K260002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260002/annotation2048713/range2048713/1

https://synbiohub.org/public/igem/BBa_K260002/annotation2048713/range2048713/1
type: Range
persistent identity: range2048713
display id: range2048713
version: 1
direction: direction/reverse
top level: BBa_K260002
start: 2445
end: 3470
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2048714
display id: annotation2048714
version: 1
top level: BBa_K260002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260002/annotation2048714/range2048714/1

https://synbiohub.org/public/igem/BBa_K260002/annotation2048714/range2048714/1
type: Range
persistent identity: range2048714
display id: range2048714
version: 1
direction: direction/reverse
top level: BBa_K260002
start: 4144
end: 4938
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation2048712
display id: annotation2048712
version: 1
top level: BBa_K260002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260002/annotation2048712/range2048712/1

https://synbiohub.org/public/igem/BBa_K260002/annotation2048712/range2048712/1
type: Range
persistent identity: range2048712
display id: range2048712
version: 1
direction: direction/reverse
top level: BBa_K260002
start: 1812
end: 2435
orientation: inline

T4_32 terminator
type: SequenceAnnotation
persistent identity: annotation2048716
display id: annotation2048716
version: 1
top level: BBa_K260002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K260002/annotation2048716/range2048716/1

https://synbiohub.org/public/igem/BBa_K260002/annotation2048716/range2048716/1
type: Range
persistent identity: range2048716
display id: range2048716
version: 1
direction: direction/forward
top level: BBa_K260002
start: 5193
end: 5224
orientation: inline

FLP(F70L)
type: SequenceAnnotation
persistent identity: annotation2048715
display id: annotation2048715
version: 1
top level: BBa_K260002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260002/annotation2048715/range2048715/1

https://synbiohub.org/public/igem/BBa_K260002/annotation2048715/range2048715/1
type: Range
persistent identity: range2048715
display id: range2048715
version: 1
direction: direction/reverse
top level: BBa_K260002
start: 5245
end: 6516
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2048721
display id: annotation2048721
version: 1
top level: BBa_K260002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260002/annotation2048721/range2048721/1

https://synbiohub.org/public/igem/BBa_K260002/annotation2048721/range2048721/1
type: Range
persistent identity: range2048721
display id: range2048721
version: 1
direction: direction/forward
top level: BBa_K260002
start: 6680
end: 6701
orientation: inline

tet-promoter
type: SequenceAnnotation
persistent identity: annotation2048720
display id: annotation2048720
version: 1
top level: BBa_K260002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K260002/annotation2048720/range2048720/1

https://synbiohub.org/public/igem/BBa_K260002/annotation2048720/range2048720/1
type: Range
persistent identity: range2048720
display id: range2048720
version: 1
direction: direction/reverse
top level: BBa_K260002
start: 6517
end: 6643
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2048691
display id: annotation2048691
version: 1
top level: BBa_K260002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260002/annotation2048691/range2048691/1

https://synbiohub.org/public/igem/BBa_K260002/annotation2048691/range2048691/1
type: Range
persistent identity: range2048691
display id: range2048691
version: 1
direction: direction/forward
top level: BBa_K260002
start: 1
end: 21
orientation: inline

BBa_K535007
type: ComponentDefinition
persistent identity: BBa K535007
display id: BBa_K535007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBBR1MCS-5 -> broad-host-range cloning vector
creator: iGEM11_UNAM-Genomics_ Mexico
created: 2011-09-24T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _701_
m user id: 0
owner id: 8698
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The plasmid we designed is a modification of the pBBR1MCS-5 , this broad-host-range (bhr) vector has been designed to assist the genetic analysis in prokaryotes, specifically in Gram- bacteria that are naturally CmR (chloramphenicol resistant). The pBBR1MCS-5 vector is relatively small (4768bp), it has an extended multiple cloning site (MCS) with 15 different restriction sites and it possesses a gentamicin resistance gene. After modification, only 3 of the 15 retrictions sites are present (see design notes). The plasmid is mobilizable when the RK2 transfer functions is provided in trans and is compatible with IncP, IncQ and IncW group plasmids, as well as with ColE1- and P15a-based replicons. We used this plasmid to transfer genes from E. coli to R. etli. First we transformed E. coli S17 with this vector containing the genes or sequences that we wanted to transfer, then we did a conjugation between our transformed E. coli cells and Rhizobium etli CFN42
mutable notes: It was amplified the BBa_J04450 including the prefix and the suffix from the psb1t3 plasmid and adding apaI and sacI restriction sites at the ends of the sequence. After, a ligation by sticky ends was with the pBBR1MCS-5 the apaI and sacI enzimes, trying to eliminate as mouch as possible of the MCS??? restriction sites We use the sequence as reported by Kovach et al. (1995).
mutable provenance: This is one of the antibiotic-resistant derivatives of the broad-host-range cloning vector pBBR1MCS.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Gentamicine resistance marker
sequence annotation: E coli his operon terminator
sequence annotation: end of terminator
sequence annotation: BioBrick prefix
sequence annotation: apaI restriction site
sequence annotation: stem loop
sequence annotation: sacI restriction site
sequence annotation: BioBrick suffix
sequence annotation: REP
sequence: https://synbiohub.org/public/igem/BBa_K535007_sequence/1

Gentamicine resistance marker
type: SequenceAnnotation
persistent identity: annotation2144895
display id: annotation2144895
version: 1
top level: BBa_K535007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K535007/annotation2144895/range2144895/1

https://synbiohub.org/public/igem/BBa_K535007/annotation2144895/range2144895/1
type: Range
persistent identity: range2144895
display id: range2144895
version: 1
direction: direction/reverse
top level: BBa_K535007
start: 3712
end: 3966
orientation: inline

E coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2144885
display id: annotation2144885
version: 1
top level: BBa_K535007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K535007/annotation2144885/range2144885/1

https://synbiohub.org/public/igem/BBa_K535007/annotation2144885/range2144885/1
type: Range
persistent identity: range2144885
display id: range2144885
version: 1
direction: direction/forward
top level: BBa_K535007
start: 22
end: 93
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2144897
display id: annotation2144897
version: 1
top level: BBa_K535007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K535007/annotation2144897/range2144897/1

https://synbiohub.org/public/igem/BBa_K535007/annotation2144897/range2144897/1
type: Range
persistent identity: range2144897
display id: range2144897
version: 1
direction: direction/forward
top level: BBa_K535007
start: 4963
end: 4967
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2144901
display id: annotation2144901
version: 1
top level: BBa_K535007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K535007/annotation2144901/range2144901/1

https://synbiohub.org/public/igem/BBa_K535007/annotation2144901/range2144901/1
type: Range
persistent identity: range2144901
display id: range2144901
version: 1
direction: direction/forward
top level: BBa_K535007
start: 5006
end: 5027
orientation: inline

apaI restriction site
type: SequenceAnnotation
persistent identity: annotation2144896
display id: annotation2144896
version: 1
top level: BBa_K535007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K535007/annotation2144896/range2144896/1

https://synbiohub.org/public/igem/BBa_K535007/annotation2144896/range2144896/1
type: Range
persistent identity: range2144896
display id: range2144896
version: 1
direction: direction/forward
top level: BBa_K535007
start: 4904
end: 4909
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation2144899
display id: annotation2144899
version: 1
top level: BBa_K535007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K535007/annotation2144899/range2144899/1

https://synbiohub.org/public/igem/BBa_K535007/annotation2144899/range2144899/1
type: Range
persistent identity: range2144899
display id: range2144899
version: 1
direction: direction/forward
top level: BBa_K535007
start: 4977
end: 4996
orientation: inline

sacI restriction site
type: SequenceAnnotation
persistent identity: annotation2144893
display id: annotation2144893
version: 1
top level: BBa_K535007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K535007/annotation2144893/range2144893/1

https://synbiohub.org/public/igem/BBa_K535007/annotation2144893/range2144893/1
type: Range
persistent identity: range2144893
display id: range2144893
version: 1
direction: direction/forward
top level: BBa_K535007
start: 232
end: 237
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2144877
display id: annotation2144877
version: 1
top level: BBa_K535007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K535007/annotation2144877/range2144877/1

https://synbiohub.org/public/igem/BBa_K535007/annotation2144877/range2144877/1
type: Range
persistent identity: range2144877
display id: range2144877
version: 1
direction: direction/forward
top level: BBa_K535007
start: 1
end: 21
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation2144886
display id: annotation2144886
version: 1
top level: BBa_K535007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K535007/annotation2144886/range2144886/1

https://synbiohub.org/public/igem/BBa_K535007/annotation2144886/range2144886/1
type: Range
persistent identity: range2144886
display id: range2144886
version: 1
direction: direction/forward
top level: BBa_K535007
start: 30
end: 65
orientation: inline

REP
type: SequenceAnnotation
persistent identity: annotation2144894
display id: annotation2144894
version: 1
top level: BBa_K535007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K535007/annotation2144894/range2144894/1

https://synbiohub.org/public/igem/BBa_K535007/annotation2144894/range2144894/1
type: Range
persistent identity: range2144894
display id: range2144894
version: 1
direction: direction/reverse
top level: BBa_K535007
start: 997
end: 1251
orientation: inline

BBa_K864000
type: ComponentDefinition
persistent identity: BBa K864000
display id: BBa_K864000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ??? antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR
sequence annotation: -10
sequence annotation: tonB terminator
sequence annotation: pSC101 ori
sequence annotation: Tandem repeat
sequence annotation: ampR BBa_P1002
sequence annotation: Mutated A>T to remove SpeI
sequence annotation: Lambda Red forward primer
sequence annotation: -35
sequence annotation: Lambda Red reverse primer
sequence annotation: Repeat "5"
sequence annotation: Lux terminator
sequence annotation: RBS
sequence annotation: T3 terminator
sequence annotation: Repeat "4"
sequence annotation: rrnC terminator
sequence annotation: repA
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_K864000_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation2198033
display id: annotation2198033
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198033/range2198033/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198033/range2198033/1
type: Range
persistent identity: range2198033
display id: range2198033
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 116
end: 135
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198007
display id: annotation2198007
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198007/range2198007/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198007/range2198007/1
type: Range
persistent identity: range2198007
display id: range2198007
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 3387
end: 3392
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2198074
display id: annotation2198074
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198074/range2198074/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198074/range2198074/1
type: Range
persistent identity: range2198074
display id: range2198074
version: 1
direction: direction/forward
top level: BBa_K864000
start: 47
end: 115
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2198057
display id: annotation2198057
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198057/range2198057/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198057/range2198057/1
type: Range
persistent identity: range2198057
display id: range2198057
version: 1
direction: direction/forward
top level: BBa_K864000
start: 190
end: 2405
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198036
display id: annotation2198036
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198036/range2198036/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198036/range2198036/1
type: Range
persistent identity: range2198036
display id: range2198036
version: 1
direction: direction/forward
top level: BBa_K864000
start: 790
end: 809
orientation: inline

ampR BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2198012
display id: annotation2198012
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198012/range2198012/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198012/range2198012/1
type: Range
persistent identity: range2198012
display id: range2198012
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 2509
end: 3369
orientation: inline

Mutated A>T to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2198029
display id: annotation2198029
version: 1
top level: BBa_K864000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198029/range2198029/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198029/range2198029/1
type: Range
persistent identity: range2198029
display id: range2198029
version: 1
direction: direction/forward
top level: BBa_K864000
start: 867
end: 867
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198030
display id: annotation2198030
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198030/range2198030/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198030/range2198030/1
type: Range
persistent identity: range2198030
display id: range2198030
version: 1
direction: direction/forward
top level: BBa_K864000
start: 2433
end: 2450
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198009
display id: annotation2198009
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198009/range2198009/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198009/range2198009/1
type: Range
persistent identity: range2198009
display id: range2198009
version: 1
direction: direction/forward
top level: BBa_K864000
start: 881
end: 886
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198006
display id: annotation2198006
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198006/range2198006/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198006/range2198006/1
type: Range
persistent identity: range2198006
display id: range2198006
version: 1
direction: direction/forward
top level: BBa_K864000
start: 903
end: 908
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198037
display id: annotation2198037
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198037/range2198037/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198037/range2198037/1
type: Range
persistent identity: range2198037
display id: range2198037
version: 1
direction: direction/forward
top level: BBa_K864000
start: 767
end: 788
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198039
display id: annotation2198039
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198039/range2198039/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198039/range2198039/1
type: Range
persistent identity: range2198039
display id: range2198039
version: 1
direction: direction/forward
top level: BBa_K864000
start: 820
end: 841
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198031
display id: annotation2198031
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198031/range2198031/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198031/range2198031/1
type: Range
persistent identity: range2198031
display id: range2198031
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 18
end: 38
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2198056
display id: annotation2198056
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198056/range2198056/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198056/range2198056/1
type: Range
persistent identity: range2198056
display id: range2198056
version: 1
direction: direction/forward
top level: BBa_K864000
start: 891
end: 912
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198075
display id: annotation2198075
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198075/range2198075/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198075/range2198075/1
type: Range
persistent identity: range2198075
display id: range2198075
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 2454
end: 2499
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2198034
display id: annotation2198034
version: 1
top level: BBa_K864000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198034/range2198034/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198034/range2198034/1
type: Range
persistent identity: range2198034
display id: range2198034
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3374
end: 3378
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198076
display id: annotation2198076
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198076/range2198076/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198076/range2198076/1
type: Range
persistent identity: range2198076
display id: range2198076
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3515
end: 3592
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2198038
display id: annotation2198038
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198038/range2198038/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198038/range2198038/1
type: Range
persistent identity: range2198038
display id: range2198038
version: 1
direction: direction/forward
top level: BBa_K864000
start: 862
end: 880
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198093
display id: annotation2198093
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198093/range2198093/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198093/range2198093/1
type: Range
persistent identity: range2198093
display id: range2198093
version: 1
direction: direction/forward
top level: BBa_K864000
start: 136
end: 175
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2198011
display id: annotation2198011
version: 1
top level: BBa_K864000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198011/range2198011/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198011/range2198011/1
type: Range
persistent identity: range2198011
display id: range2198011
version: 1
direction: direction/forward
top level: BBa_K864000
start: 939
end: 1889
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198032
display id: annotation2198032
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198032/range2198032/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198032/range2198032/1
type: Range
persistent identity: range2198032
display id: range2198032
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3495
end: 3514
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198010
display id: annotation2198010
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198010/range2198010/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198010/range2198010/1
type: Range
persistent identity: range2198010
display id: range2198010
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 3413
end: 3418
orientation: inline

BBa_J119050
type: ComponentDefinition
persistent identity: BBa J119050
display id: BBa_J119050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4K8
creator: Todd Eckdahl and A. Malcolm Campbell
created: 2012-01-23T12:00:00Z
modified: 2015-08-31T04:08:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Insulated low copy vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. Uses I50042 low copy pSC101 replication origin.
mutable notes: Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.
mutable provenance: de novo DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR primer
sequence annotation: Kanamycin resistance gene
sequence annotation: B0010 terminator
sequence annotation: stuffer
sequence annotation: B0025 terminator
sequence annotation: Prefix
sequence annotation: Suffix
sequence annotation: I50042 low copy pSC101 origin
sequence annotation: VF2 primer
sequence: https://synbiohub.org/public/igem/BBa_J119050_sequence/1

VR primer
type: SequenceAnnotation
persistent identity: annotation2169727
display id: annotation2169727
version: 1
top level: BBa_J119050
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119050/annotation2169727/range2169727/1

https://synbiohub.org/public/igem/BBa_J119050/annotation2169727/range2169727/1
type: Range
persistent identity: range2169727
display id: range2169727
version: 1
direction: direction/forward
top level: BBa_J119050
start: 208
end: 227
orientation: inline

Kanamycin resistance gene
type: SequenceAnnotation
persistent identity: annotation2169731
display id: annotation2169731
version: 1
top level: BBa_J119050
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J119050/annotation2169731/range2169731/1

https://synbiohub.org/public/igem/BBa_J119050/annotation2169731/range2169731/1
type: Range
persistent identity: range2169731
display id: range2169731
version: 1
direction: direction/reverse
top level: BBa_J119050
start: 2571
end: 3386
orientation: inline

B0010 terminator
type: SequenceAnnotation
persistent identity: annotation2169729
display id: annotation2169729
version: 1
top level: BBa_J119050
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J119050/annotation2169729/range2169729/1

https://synbiohub.org/public/igem/BBa_J119050/annotation2169729/range2169729/1
type: Range
persistent identity: range2169729
display id: range2169729
version: 1
direction: direction/forward
top level: BBa_J119050
start: 3561
end: 3638
orientation: inline

stuffer
type: SequenceAnnotation
persistent identity: annotation2169725
display id: annotation2169725
version: 1
top level: BBa_J119050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119050/annotation2169725/range2169725/1

https://synbiohub.org/public/igem/BBa_J119050/annotation2169725/range2169725/1
type: Range
persistent identity: range2169725
display id: range2169725
version: 1
direction: direction/forward
top level: BBa_J119050
start: 3701
end: 3712
orientation: inline

B0025 terminator
type: SequenceAnnotation
persistent identity: annotation2169728
display id: annotation2169728
version: 1
top level: BBa_J119050
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J119050/annotation2169728/range2169728/1

https://synbiohub.org/public/igem/BBa_J119050/annotation2169728/range2169728/1
type: Range
persistent identity: range2169728
display id: range2169728
version: 1
direction: direction/forward
top level: BBa_J119050
start: 22
end: 205
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2169724
display id: annotation2169724
version: 1
top level: BBa_J119050
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119050/annotation2169724/range2169724/1

https://synbiohub.org/public/igem/BBa_J119050/annotation2169724/range2169724/1
type: Range
persistent identity: range2169724
display id: range2169724
version: 1
direction: direction/forward
top level: BBa_J119050
start: 3679
end: 3700
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2169723
display id: annotation2169723
version: 1
top level: BBa_J119050
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119050/annotation2169723/range2169723/1

https://synbiohub.org/public/igem/BBa_J119050/annotation2169723/range2169723/1
type: Range
persistent identity: range2169723
display id: range2169723
version: 1
direction: direction/forward
top level: BBa_J119050
start: 1
end: 21
orientation: inline

I50042 low copy pSC101 origin
type: SequenceAnnotation
persistent identity: annotation2169730
display id: annotation2169730
version: 1
top level: BBa_J119050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119050/annotation2169730/range2169730/1

https://synbiohub.org/public/igem/BBa_J119050/annotation2169730/range2169730/1
type: Range
persistent identity: range2169730
display id: range2169730
version: 1
direction: direction/forward
top level: BBa_J119050
start: 310
end: 2346
orientation: inline

VF2 primer
type: SequenceAnnotation
persistent identity: annotation2169726
display id: annotation2169726
version: 1
top level: BBa_J119050
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119050/annotation2169726/range2169726/1

https://synbiohub.org/public/igem/BBa_J119050/annotation2169726/range2169726/1
type: Range
persistent identity: range2169726
display id: range2169726
version: 1
direction: direction/forward
top level: BBa_J119050
start: 3541
end: 3560
orientation: inline

BBa_K245005
type: ComponentDefinition
persistent identity: BBa K245005
display id: BBa_K245005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BB-NIC-II-HisN
creator: Nika Debeljak
created: 2009-09-23T11:00:00Z
modified: 2015-05-08T01:11:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _337_
m user id: 0
owner id: 4964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BB-NIC-II-HisN originates from BioBrick vector pSB1A2 but contains modified prefix and suffix according to assembly standard 37. T7 promoter, RBS and ATG are included in prefix and T7 terminator and STOP codon in suffix. His tag is also inserted between ATG and multiple cloning site in prefix. There are four new inner restriction sites added in multiple-cloning site (NgoMIV and AgeI in prefix; XmaI and BspEI in suffix) which enable extension of part cloned into BB-NIC-II-HisN with SG on N-terminal or TG on C-terminal; extension of linker and creating a friendly scar during parts fusion.
mutable notes: BioBrick vector pSB1A2 was first cut with EcoRI/PstI. An insert containing modified prefix and suffix (according to assembly standard 37) was ligated in between. Subsequently a ccdB domain was inserted between AgeI and XmaI restriction sites.
mutable provenance: The source is BioBrick vector pSB1A2; modified prefix and suffix sequences are of synthetic origin; ccdB originates from part BBa_P1010.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: SpeI
sequence annotation: XmaI
sequence annotation: PstI
sequence annotation: T7 promoter
sequence annotation: lacO regulatory site
sequence annotation: XbaI
sequence annotation: BspEI
sequence annotation: pBR233 origin
sequence annotation: amp resistance marker
sequence annotation: His tag
sequence annotation: modified suffix
sequence annotation: rbs
sequence annotation: NgoMIV
sequence annotation: START
sequence annotation: EcoRI
sequence annotation: NotI
sequence annotation: AgeI
sequence annotation: T7 terminator
sequence annotation: STOP
sequence annotation: amp promoter
sequence annotation: modified prefix
sequence: https://synbiohub.org/public/igem/BBa_K245005_sequence/1

SpeI
type: SequenceAnnotation
persistent identity: annotation2026902
display id: annotation2026902
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026902/range2026902/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026902/range2026902/1
type: Range
persistent identity: range2026902
display id: range2026902
version: 1
direction: direction/forward
top level: BBa_K245005
start: 13
end: 18
orientation: inline

XmaI
type: SequenceAnnotation
persistent identity: annotation2026900
display id: annotation2026900
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026900/range2026900/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026900/range2026900/1
type: Range
persistent identity: range2026900
display id: range2026900
version: 1
direction: direction/forward
top level: BBa_K245005
start: 1
end: 6
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2026906
display id: annotation2026906
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026906/range2026906/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026906/range2026906/1
type: Range
persistent identity: range2026906
display id: range2026906
version: 1
direction: direction/forward
top level: BBa_K245005
start: 77
end: 82
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2026913
display id: annotation2026913
version: 1
top level: BBa_K245005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026913/range2026913/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026913/range2026913/1
type: Range
persistent identity: range2026913
display id: range2026913
version: 1
direction: direction/forward
top level: BBa_K245005
start: 2133
end: 2151
orientation: inline

lacO regulatory site
type: SequenceAnnotation
persistent identity: annotation2026914
display id: annotation2026914
version: 1
top level: BBa_K245005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026914/range2026914/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026914/range2026914/1
type: Range
persistent identity: range2026914
display id: range2026914
version: 1
direction: direction/forward
top level: BBa_K245005
start: 2151
end: 2178
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2026918
display id: annotation2026918
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026918/range2026918/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026918/range2026918/1
type: Range
persistent identity: range2026918
display id: range2026918
version: 1
direction: direction/forward
top level: BBa_K245005
start: 2244
end: 2249
orientation: inline

BspEI
type: SequenceAnnotation
persistent identity: annotation2026901
display id: annotation2026901
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026901/range2026901/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026901/range2026901/1
type: Range
persistent identity: range2026901
display id: range2026901
version: 1
direction: direction/forward
top level: BBa_K245005
start: 7
end: 12
orientation: inline

pBR233 origin
type: SequenceAnnotation
persistent identity: annotation2026907
display id: annotation2026907
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026907/range2026907/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026907/range2026907/1
type: Range
persistent identity: range2026907
display id: range2026907
version: 1
direction: direction/reverse
top level: BBa_K245005
start: 244
end: 863
orientation: inline

amp resistance marker
type: SequenceAnnotation
persistent identity: annotation2026908
display id: annotation2026908
version: 1
top level: BBa_K245005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026908/range2026908/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026908/range2026908/1
type: Range
persistent identity: range2026908
display id: range2026908
version: 1
direction: direction/reverse
top level: BBa_K245005
start: 1018
end: 1878
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2026917
display id: annotation2026917
version: 1
top level: BBa_K245005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026917/range2026917/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026917/range2026917/1
type: Range
persistent identity: range2026917
display id: range2026917
version: 1
direction: direction/forward
top level: BBa_K245005
start: 2226
end: 2243
orientation: inline

modified suffix
type: SequenceAnnotation
persistent identity: annotation2026899
display id: annotation2026899
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026899/range2026899/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026899/range2026899/1
type: Range
persistent identity: range2026899
display id: range2026899
version: 1
direction: direction/forward
top level: BBa_K245005
start: 1
end: 82
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2026915
display id: annotation2026915
version: 1
top level: BBa_K245005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026915/range2026915/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026915/range2026915/1
type: Range
persistent identity: range2026915
display id: range2026915
version: 1
direction: direction/forward
top level: BBa_K245005
start: 2208
end: 2214
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2026919
display id: annotation2026919
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026919/range2026919/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026919/range2026919/1
type: Range
persistent identity: range2026919
display id: range2026919
version: 1
direction: direction/forward
top level: BBa_K245005
start: 2250
end: 2255
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2026916
display id: annotation2026916
version: 1
top level: BBa_K245005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026916/range2026916/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026916/range2026916/1
type: Range
persistent identity: range2026916
display id: range2026916
version: 1
direction: direction/forward
top level: BBa_K245005
start: 2220
end: 2222
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2026911
display id: annotation2026911
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026911/range2026911/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026911/range2026911/1
type: Range
persistent identity: range2026911
display id: range2026911
version: 1
direction: direction/forward
top level: BBa_K245005
start: 2119
end: 2124
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation2026912
display id: annotation2026912
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026912/range2026912/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026912/range2026912/1
type: Range
persistent identity: range2026912
display id: range2026912
version: 1
direction: direction/forward
top level: BBa_K245005
start: 2125
end: 2132
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2026920
display id: annotation2026920
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026920/range2026920/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026920/range2026920/1
type: Range
persistent identity: range2026920
display id: range2026920
version: 1
direction: direction/forward
top level: BBa_K245005
start: 2256
end: 2261
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2026904
display id: annotation2026904
version: 1
top level: BBa_K245005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026904/range2026904/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026904/range2026904/1
type: Range
persistent identity: range2026904
display id: range2026904
version: 1
direction: direction/forward
top level: BBa_K245005
start: 22
end: 69
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation2026905
display id: annotation2026905
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026905/range2026905/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026905/range2026905/1
type: Range
persistent identity: range2026905
display id: range2026905
version: 1
direction: direction/forward
top level: BBa_K245005
start: 70
end: 77
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2026903
display id: annotation2026903
version: 1
top level: BBa_K245005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026903/range2026903/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026903/range2026903/1
type: Range
persistent identity: range2026903
display id: range2026903
version: 1
direction: direction/forward
top level: BBa_K245005
start: 19
end: 21
orientation: inline

amp promoter
type: SequenceAnnotation
persistent identity: annotation2026909
display id: annotation2026909
version: 1
top level: BBa_K245005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026909/range2026909/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026909/range2026909/1
type: Range
persistent identity: range2026909
display id: range2026909
version: 1
direction: direction/reverse
top level: BBa_K245005
start: 1920
end: 1948
orientation: inline

modified prefix
type: SequenceAnnotation
persistent identity: annotation2026910
display id: annotation2026910
version: 1
top level: BBa_K245005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245005/annotation2026910/range2026910/1

https://synbiohub.org/public/igem/BBa_K245005/annotation2026910/range2026910/1
type: Range
persistent identity: range2026910
display id: range2026910
version: 1
direction: direction/forward
top level: BBa_K245005
start: 2119
end: 2261
orientation: inline

BBa_K572200
type: ComponentDefinition
persistent identity: BBa K572200
display id: BBa_K572200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Light based expression vector- pSB1Pe
creator: Chaitra Prabhakara
created: 2011-10-04T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
group u list: _743_
m user id: 0
owner id: 8355
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB1c3 is changed to pSB1Pe by replacing the antibiotic gene with proteorhodopsin. The plasmid also has a carbon stress promoter- PcstA and double terminator - B0015. The EXSP sites are placed within the promoter and the double terminator region so as to allow cloning of recombinant protein into the plasmid and expressing the same in low carbon conditions.
mutable notes: The reading frame should be considered while designing
mutable provenance: Plasmid Backbone - Psb1c3 Parts - B0015, K118011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K572200_sequence/1

BBa_K1680014
type: ComponentDefinition
persistent identity: BBa K1680014
display id: BBa_K1680014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS313 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:47:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.
mutable notes: contains an ADH Terminator and restrictions sites for the RFC10 standard only in the multiple cloning site, no restrictions sites for RCF10 enzymes in the backbone.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ampR promotor
sequence annotation: CEN/ARS yeast centromere
sequence annotation: ampR
sequence annotation: pUC origin
sequence annotation: T3 promotor
sequence annotation: RFC10 MCS
sequence annotation: f1 ori
sequence annotation: T7 promotor
sequence annotation: HIS3 promotor
sequence annotation: lacZalpha
sequence annotation: lac promotor
sequence annotation: HIS3
sequence: https://synbiohub.org/public/igem/BBa_K1680014_sequence/1

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466130
display id: annotation2466130
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466130/range2466130/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466130/range2466130/1
type: Range
persistent identity: range2466130
display id: range2466130
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 4215
end: 4319
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466131
display id: annotation2466131
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466131/range2466131/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466131/range2466131/1
type: Range
persistent identity: range2466131
display id: range2466131
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 4356
end: 4859
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466129
display id: annotation2466129
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466129/range2466129/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466129/range2466129/1
type: Range
persistent identity: range2466129
display id: range2466129
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 3354
end: 4214
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466128
display id: annotation2466128
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466128/range2466128/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466128/range2466128/1
type: Range
persistent identity: range2466128
display id: range2466128
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2595
end: 3183
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466125
display id: annotation2466125
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466125/range2466125/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466125/range2466125/1
type: Range
persistent identity: range2466125
display id: range2466125
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2153
end: 2172
orientation: inline

RFC10 MCS
type: SequenceAnnotation
persistent identity: annotation2466135
display id: annotation2466135
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466135/range2466135/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466135/range2466135/1
type: Range
persistent identity: range2466135
display id: range2466135
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 2083
end: 2125
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation2466099
display id: annotation2466099
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466099/range2466099/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466099/range2466099/1
type: Range
persistent identity: range2466099
display id: range2466099
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 1423
end: 1878
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466133
display id: annotation2466133
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466133/range2466133/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466133/range2466133/1
type: Range
persistent identity: range2466133
display id: range2466133
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 2046
end: 2065
orientation: inline

HIS3 promotor
type: SequenceAnnotation
persistent identity: annotation2466090
display id: annotation2466090
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466090/range2466090/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466090/range2466090/1
type: Range
persistent identity: range2466090
display id: range2466090
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 315
end: 502
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466100
display id: annotation2466100
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466100/range2466100/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466100/range2466100/1
type: Range
persistent identity: range2466100
display id: range2466100
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 1661
end: 2077
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466127
display id: annotation2466127
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466127/range2466127/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466127/range2466127/1
type: Range
persistent identity: range2466127
display id: range2466127
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2241
end: 2271
orientation: inline

HIS3
type: SequenceAnnotation
persistent identity: annotation2466098
display id: annotation2466098
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466098/range2466098/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466098/range2466098/1
type: Range
persistent identity: range2466098
display id: range2466098
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 503
end: 1162
orientation: inline

BBa_K345669
type: ComponentDefinition
persistent identity: BBa K345669
display id: BBa_K345669
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psb1c3 -> BsiWI
creator: sebastian Ramov
created: 2010-11-05T12:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
group u list: _547_
m user id: 0
owner id: 4399
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a new backbone modified from psb1c3 to facilitate ligation independent cloning. This new backbone allows creation of parts and composites into any desired cloning standard.
mutable notes: The EcoRI and PstI restriction sites are inactivated by mutagenesis so as not to create two sets of these restriction sites. A BsiWI restriction site is created between the XbaI and SpeI restriction sites for ligation independent cloning.
mutable provenance: pSB1C3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: chloramphenicol resistance
sequence annotation: verification reverse(VR) primer binding site
sequence annotation: LIC site (BsiWI)
sequence annotation: verification forward (VF2) primer binding site
sequence annotation: end of terminator
sequence annotation: rep(pMB1)
sequence annotation: T0 terminator
sequence annotation: stem loop
sequence annotation: E.coli his operon terminator
sequence: https://synbiohub.org/public/igem/BBa_K345669_sequence/1

chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2114487
display id: annotation2114487
version: 1
top level: BBa_K345669
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K345669/annotation2114487/range2114487/1

https://synbiohub.org/public/igem/BBa_K345669/annotation2114487/range2114487/1
type: Range
persistent identity: range2114487
display id: range2114487
version: 1
direction: direction/forward
top level: BBa_K345669
start: 1262
end: 1831
orientation: inline

verification reverse(VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation2114484
display id: annotation2114484
version: 1
top level: BBa_K345669
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K345669/annotation2114484/range2114484/1

https://synbiohub.org/public/igem/BBa_K345669/annotation2114484/range2114484/1
type: Range
persistent identity: range2114484
display id: range2114484
version: 1
direction: direction/forward
top level: BBa_K345669
start: 157
end: 176
orientation: inline

LIC site (BsiWI)
type: SequenceAnnotation
persistent identity: annotation2114482
display id: annotation2114482
version: 1
top level: BBa_K345669
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K345669/annotation2114482/range2114482/1

https://synbiohub.org/public/igem/BBa_K345669/annotation2114482/range2114482/1
type: Range
persistent identity: range2114482
display id: range2114482
version: 1
direction: direction/forward
top level: BBa_K345669
start: 2071
end: 2072
orientation: inline

verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation2114488
display id: annotation2114488
version: 1
top level: BBa_K345669
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K345669/annotation2114488/range2114488/1

https://synbiohub.org/public/igem/BBa_K345669/annotation2114488/range2114488/1
type: Range
persistent identity: range2114488
display id: range2114488
version: 1
direction: direction/forward
top level: BBa_K345669
start: 1932
end: 1952
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2114489
display id: annotation2114489
version: 1
top level: BBa_K345669
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K345669/annotation2114489/range2114489/1

https://synbiohub.org/public/igem/BBa_K345669/annotation2114489/range2114489/1
type: Range
persistent identity: range2114489
display id: range2114489
version: 1
direction: direction/forward
top level: BBa_K345669
start: 2009
end: 2012
orientation: inline

rep(pMB1)
type: SequenceAnnotation
persistent identity: annotation2114485
display id: annotation2114485
version: 1
top level: BBa_K345669
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K345669/annotation2114485/range2114485/1

https://synbiohub.org/public/igem/BBa_K345669/annotation2114485/range2114485/1
type: Range
persistent identity: range2114485
display id: range2114485
version: 1
direction: direction/forward
top level: BBa_K345669
start: 261
end: 875
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2114486
display id: annotation2114486
version: 1
top level: BBa_K345669
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K345669/annotation2114486/range2114486/1

https://synbiohub.org/public/igem/BBa_K345669/annotation2114486/range2114486/1
type: Range
persistent identity: range2114486
display id: range2114486
version: 1
direction: direction/forward
top level: BBa_K345669
start: 1032
end: 1147
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation2114490
display id: annotation2114490
version: 1
top level: BBa_K345669
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K345669/annotation2114490/range2114490/1

https://synbiohub.org/public/igem/BBa_K345669/annotation2114490/range2114490/1
type: Range
persistent identity: range2114490
display id: range2114490
version: 1
direction: direction/forward
top level: BBa_K345669
start: 2022
end: 2041
orientation: inline

LIC site (BsiWI)
type: SequenceAnnotation
persistent identity: annotation2114481
display id: annotation2114481
version: 1
top level: BBa_K345669
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K345669/annotation2114481/range2114481/1

https://synbiohub.org/public/igem/BBa_K345669/annotation2114481/range2114481/1
type: Range
persistent identity: range2114481
display id: range2114481
version: 1
direction: direction/forward
top level: BBa_K345669
start: 1
end: 4
orientation: inline

E.coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2114483
display id: annotation2114483
version: 1
top level: BBa_K345669
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K345669/annotation2114483/range2114483/1

https://synbiohub.org/public/igem/BBa_K345669/annotation2114483/range2114483/1
type: Range
persistent identity: range2114483
display id: range2114483
version: 1
direction: direction/forward
top level: BBa_K345669
start: 22
end: 114
orientation: inline

pSBBs4S
type: ComponentDefinition
persistent identity: BBa K823022
display id: BBa_K823022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs4S: Empty backbone for integration into Bacillus subtilis thrC locus
creator: Jara Radeck
created: 2012-08-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Spectinomycin cassette. It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1731.
mutable notes: This plasmid can be used as empty backbone vectors like pSB1C3 but is able to integrate in B.subtilis. The clonging site also includes a rfp for selection.
mutable provenance: B.subtilis vector pDG1731
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: bla
sequence annotation: 'thrC
sequence annotation: thrC'
sequence annotation: ori approx.
sequence annotation: spc
sequence annotation: thrB
sequence annotation: hom
sequence: https://synbiohub.org/public/igem/BBa_K823022_sequence/1

bla
type: SequenceAnnotation
persistent identity: annotation2179611
display id: annotation2179611
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179611/range2179611/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179611/range2179611/1
type: Range
persistent identity: range2179611
display id: range2179611
version: 1
direction: direction/reverse
top level: pSBBs4S
start: 2716
end: 3576
orientation: inline

'thrC
type: SequenceAnnotation
persistent identity: annotation2179608
display id: annotation2179608
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179608/range2179608/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179608/range2179608/1
type: Range
persistent identity: range2179608
display id: range2179608
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1028
end: 1456
orientation: inline

thrC'
type: SequenceAnnotation
persistent identity: annotation2179613
display id: annotation2179613
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179613/range2179613/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179613/range2179613/1
type: Range
persistent identity: range2179613
display id: range2179613
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4033
end: 4542
orientation: inline

ori approx.
type: SequenceAnnotation
persistent identity: annotation2179610
display id: annotation2179610
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179610/range2179610/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179610/range2179610/1
type: Range
persistent identity: range2179610
display id: range2179610
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1942
end: 2556
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2179607
display id: annotation2179607
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179607/range2179607/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179607/range2179607/1
type: Range
persistent identity: range2179607
display id: range2179607
version: 1
direction: direction/forward
top level: pSBBs4S
start: 211
end: 885
orientation: inline

thrB
type: SequenceAnnotation
persistent identity: annotation2179609
display id: annotation2179609
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179609/range2179609/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179609/range2179609/1
type: Range
persistent identity: range2179609
display id: range2179609
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1453
end: 1863
orientation: inline

hom
type: SequenceAnnotation
persistent identity: annotation2179612
display id: annotation2179612
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179612/range2179612/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179612/range2179612/1
type: Range
persistent identity: range2179612
display id: range2179612
version: 1
direction: direction/forward
top level: pSBBs4S
start: 3731
end: 4033
orientation: inline

BBa_K371051
type: ComponentDefinition
persistent identity: BBa K371051
display id: BBa_K371051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C5 (1bp mutation of pSB1C3 to remove SacI)
creator: Ruijun Zhu
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 7269
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is a plasmid bone, which has only a single site mutation compared with pSB1C3. The SacI site is mutated to allow this plasmid compatible to BBF EFC 53 standard.
mutable notes: In order to make our working plasmid compatible to BBF RFC 53, we search the restriction enzymen site in pSB1C3 plasmid. And we found a SacI site in the pSB1C3. The site is between downstream of chloramphenicol and the T0 terminator. We think the mutation does not affect the regular function of pSB1C3 plasmid. So all of our following experiment of fusion protein was done in this new plasmid.
mutable provenance: this part comes from the partsregistry BBa_pSB1C3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Stem loop
sequence annotation: rep (pMB1)
sequence annotation: C->T mutation
sequence annotation: A->G silent mutation
sequence annotation: prefix
sequence annotation: Chloramphenicol resistance
sequence annotation: suffix
sequence annotation: Extra C discovered in sequencing
sequence annotation: C->G silent muation
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: Deletion of AC
sequence annotation: end of terminator
sequence annotation: T0 terminator
sequence annotation: ORI
sequence annotation: E. coli his operon terminator
sequence: https://synbiohub.org/public/igem/BBa_K371051_sequence/1

Stem loop
type: SequenceAnnotation
persistent identity: annotation2113442
display id: annotation2113442
version: 1
top level: BBa_K371051
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113442/range2113442/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113442/range2113442/1
type: Range
persistent identity: range2113442
display id: range2113442
version: 1
direction: direction/forward
top level: BBa_K371051
start: 30
end: 64
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2113445
display id: annotation2113445
version: 1
top level: BBa_K371051
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113445/range2113445/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113445/range2113445/1
type: Range
persistent identity: range2113445
display id: range2113445
version: 1
direction: direction/forward
top level: BBa_K371051
start: 261
end: 875
orientation: inline

C->T mutation
type: SequenceAnnotation
persistent identity: annotation2113452
display id: annotation2113452
version: 1
top level: BBa_K371051
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113452/range2113452/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113452/range2113452/1
type: Range
persistent identity: range2113452
display id: range2113452
version: 1
direction: direction/forward
top level: BBa_K371051
start: 1939
end: 1939
orientation: inline

A->G silent mutation
type: SequenceAnnotation
persistent identity: annotation2113450
display id: annotation2113450
version: 1
top level: BBa_K371051
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113450/range2113450/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113450/range2113450/1
type: Range
persistent identity: range2113450
display id: range2113450
version: 1
direction: direction/forward
top level: BBa_K371051
start: 1237
end: 1237
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2113455
display id: annotation2113455
version: 1
top level: BBa_K371051
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113455/range2113455/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113455/range2113455/1
type: Range
persistent identity: range2113455
display id: range2113455
version: 1
direction: direction/forward
top level: BBa_K371051
start: 2051
end: 2072
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2113449
display id: annotation2113449
version: 1
top level: BBa_K371051
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113449/range2113449/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113449/range2113449/1
type: Range
persistent identity: range2113449
display id: range2113449
version: 1
direction: direction/forward
top level: BBa_K371051
start: 1162
end: 1821
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2113440
display id: annotation2113440
version: 1
top level: BBa_K371051
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113440/range2113440/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113440/range2113440/1
type: Range
persistent identity: range2113440
display id: range2113440
version: 1
direction: direction/forward
top level: BBa_K371051
start: 1
end: 21
orientation: inline

Extra C discovered in sequencing
type: SequenceAnnotation
persistent identity: annotation2113451
display id: annotation2113451
version: 1
top level: BBa_K371051
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113451/range2113451/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113451/range2113451/1
type: Range
persistent identity: range2113451
display id: range2113451
version: 1
direction: direction/forward
top level: BBa_K371051
start: 1919
end: 1919
orientation: inline

C->G silent muation
type: SequenceAnnotation
persistent identity: annotation2113448
display id: annotation2113448
version: 1
top level: BBa_K371051
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113448/range2113448/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113448/range2113448/1
type: Range
persistent identity: range2113448
display id: range2113448
version: 1
direction: direction/forward
top level: BBa_K371051
start: 1149
end: 1149
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation2113444
display id: annotation2113444
version: 1
top level: BBa_K371051
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113444/range2113444/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113444/range2113444/1
type: Range
persistent identity: range2113444
display id: range2113444
version: 1
direction: direction/forward
top level: BBa_K371051
start: 157
end: 176
orientation: inline

Deletion of AC
type: SequenceAnnotation
persistent identity: annotation2113443
display id: annotation2113443
version: 1
top level: BBa_K371051
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113443/range2113443/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113443/range2113443/1
type: Range
persistent identity: range2113443
display id: range2113443
version: 1
direction: direction/forward
top level: BBa_K371051
start: 34
end: 35
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2113453
display id: annotation2113453
version: 1
top level: BBa_K371051
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113453/range2113453/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113453/range2113453/1
type: Range
persistent identity: range2113453
display id: range2113453
version: 1
direction: direction/forward
top level: BBa_K371051
start: 2009
end: 2012
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2113454
display id: annotation2113454
version: 1
top level: BBa_K371051
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113454/range2113454/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113454/range2113454/1
type: Range
persistent identity: range2113454
display id: range2113454
version: 1
direction: direction/forward
top level: BBa_K371051
start: 2022
end: 2041
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2113447
display id: annotation2113447
version: 1
top level: BBa_K371051
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113447/range2113447/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113447/range2113447/1
type: Range
persistent identity: range2113447
display id: range2113447
version: 1
direction: direction/forward
top level: BBa_K371051
start: 1042
end: 1147
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2113446
display id: annotation2113446
version: 1
top level: BBa_K371051
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113446/range2113446/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113446/range2113446/1
type: Range
persistent identity: range2113446
display id: range2113446
version: 1
direction: direction/forward
top level: BBa_K371051
start: 276
end: 276
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2113441
display id: annotation2113441
version: 1
top level: BBa_K371051
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K371051/annotation2113441/range2113441/1

https://synbiohub.org/public/igem/BBa_K371051/annotation2113441/range2113441/1
type: Range
persistent identity: range2113441
display id: range2113441
version: 1
direction: direction/forward
top level: BBa_K371051
start: 22
end: 93
orientation: inline

BBa_K382005
type: ComponentDefinition
persistent identity: BBa K382005
display id: BBa_K382005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE GFP Reporter Series Vector with BioBrick MCS (Kan resistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:18:36Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has constitutive plant promoter and in-frame GFP reporter. Conforms to Silver BIoBrick Assembly Standard 21.
mutable notes: Silver Standard 21
mutable provenance: Modified vector pORE_R2 from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K382005_sequence/1

BBa_K382001
type: ComponentDefinition
persistent identity: BBa K382001
display id: BBa_K382001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE Open Serices Vector with BioBrick MCS (Kan resistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:19:10Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has no promoter or reporter. Conforms to Silver BioBrick Assembly Standard 21.
mutable notes: Silver Assembly Standard 21
mutable provenance: Modified vector pORE_O1 from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K382001_sequence/1

BBa_K1438032
type: ComponentDefinition
persistent identity: BBa K1438032
display id: BBa_K1438032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PJS418
creator: Johann Bauerfeind
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:25Z
discontinued: false
dominant: false
group u list: _1816_
m user id: 0
owner id: 19714
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pJS418
mutable notes: pJS418
mutable provenance: pJS418
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1438032_sequence/1

BBa_K1084201
type: ComponentDefinition
persistent identity: BBa K1084201
display id: BBa_K1084201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: POK
creator: Ami Kawahata
created: 2013-08-26T11:00:00Z
modified: 2015-05-08T01:09:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1394_
m user id: 0
owner id: 11723
owning group id: 9
sample status: No part sequence
status: status/Deleted
bookmark: false
mutable description: coming soon...
mutable notes: coming soon ...
mutable provenance: coming soon...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

pSB1A8YL
type: ComponentDefinition
persistent identity: BBa K2117009
display id: BBa_K2117009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid enabling Yarrowia lipolytica biobrick
creator: Isabella Loft
created: 2016-10-13T11:00:00Z
modified: 2016-10-17T01:27:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2585_
m user id: 31011
owner id: 31018
owning group id: 9
status: status/Planning
bookmark: false
mutable description: will
mutable notes: will
mutable provenance: will
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: C to A
sequence annotation: SB-prep-pSB1A8YL-fw
sequence annotation: VR
sequence annotation: pAmpR
sequence annotation: C to G
sequence annotation: 140
sequence annotation: AmpR (ampicillin selection marker)
sequence annotation: SB-prep-pSB1A8YL-re
sequence annotation: BioBrick prefix
sequence annotation: T to C
sequence annotation: ORI1001 origin of replication
sequence annotation: BioBrick suffix
sequence annotation: pURA3
sequence annotation: pBR322 origin of replication
sequence annotation: CYC1 terminator
sequence annotation: URA3 (uracil selection marker)
sequence annotation: Cen1-1 origin of replication
sequence: https://synbiohub.org/public/igem/BBa_K2117009_sequence/1

C to A
type: SequenceAnnotation
persistent identity: annotation2516564
display id: annotation2516564
version: 1
top level: pSB1A8YL
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516564/range2516564/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516564/range2516564/1
type: Range
persistent identity: range2516564
display id: range2516564
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 1246
end: 1246
orientation: inline

SB-prep-pSB1A8YL-fw
type: SequenceAnnotation
persistent identity: annotation2516559
display id: annotation2516559
version: 1
top level: pSB1A8YL
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516559/range2516559/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516559/range2516559/1
type: Range
persistent identity: range2516559
display id: range2516559
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 14
end: 43
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2516573
display id: annotation2516573
version: 1
top level: pSB1A8YL
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516573/range2516573/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516573/range2516573/1
type: Range
persistent identity: range2516573
display id: range2516573
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 3993
end: 4012
orientation: inline

pAmpR
type: SequenceAnnotation
persistent identity: annotation2516570
display id: annotation2516570
version: 1
top level: pSB1A8YL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516570/range2516570/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516570/range2516570/1
type: Range
persistent identity: range2516570
display id: range2516570
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 2175
end: 2203
orientation: inline

C to G
type: SequenceAnnotation
persistent identity: annotation2516566
display id: annotation2516566
version: 1
top level: pSB1A8YL
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516566/range2516566/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516566/range2516566/1
type: Range
persistent identity: range2516566
display id: range2516566
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 1438
end: 1438
orientation: inline

140
type: SequenceAnnotation
persistent identity: annotation2516561
display id: annotation2516561
version: 1
top level: pSB1A8YL
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516561/range2516561/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516561/range2516561/1
type: Range
persistent identity: range2516561
display id: range2516561
version: 1
direction: direction/reverse
top level: pSB1A8YL
start: 271
end: 290
orientation: inline

AmpR (ampicillin selection marker)
type: SequenceAnnotation
persistent identity: annotation2516571
display id: annotation2516571
version: 1
top level: pSB1A8YL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516571/range2516571/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516571/range2516571/1
type: Range
persistent identity: range2516571
display id: range2516571
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 2245
end: 3105
orientation: inline

SB-prep-pSB1A8YL-re
type: SequenceAnnotation
persistent identity: annotation2516574
display id: annotation2516574
version: 1
top level: pSB1A8YL
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516574/range2516574/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516574/range2516574/1
type: Range
persistent identity: range2516574
display id: range2516574
version: 1
direction: direction/reverse
top level: pSB1A8YL
start: 3997
end: 4020
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2516575
display id: annotation2516575
version: 1
top level: pSB1A8YL
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516575/range2516575/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516575/range2516575/1
type: Range
persistent identity: range2516575
display id: range2516575
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 4014
end: 4034
orientation: inline

T to C
type: SequenceAnnotation
persistent identity: annotation2516565
display id: annotation2516565
version: 1
top level: pSB1A8YL
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516565/range2516565/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516565/range2516565/1
type: Range
persistent identity: range2516565
display id: range2516565
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 1405
end: 1405
orientation: inline

ORI1001 origin of replication
type: SequenceAnnotation
persistent identity: annotation2516569
display id: annotation2516569
version: 1
top level: pSB1A8YL
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516569/range2516569/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516569/range2516569/1
type: Range
persistent identity: range2516569
display id: range2516569
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 1693
end: 1806
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2516558
display id: annotation2516558
version: 1
top level: pSB1A8YL
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516558/range2516558/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516558/range2516558/1
type: Range
persistent identity: range2516558
display id: range2516558
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 1
end: 21
orientation: inline

pURA3
type: SequenceAnnotation
persistent identity: annotation2516567
display id: annotation2516567
version: 1
top level: pSB1A8YL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516567/range2516567/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516567/range2516567/1
type: Range
persistent identity: range2516567
display id: range2516567
version: 1
direction: direction/reverse
top level: pSB1A8YL
start: 1456
end: 1540
orientation: inline

pBR322 origin of replication
type: SequenceAnnotation
persistent identity: annotation2516572
display id: annotation2516572
version: 1
top level: pSB1A8YL
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516572/range2516572/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516572/range2516572/1
type: Range
persistent identity: range2516572
display id: range2516572
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 3260
end: 3879
orientation: inline

CYC1 terminator
type: SequenceAnnotation
persistent identity: annotation2516560
display id: annotation2516560
version: 1
top level: pSB1A8YL
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516560/range2516560/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516560/range2516560/1
type: Range
persistent identity: range2516560
display id: range2516560
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 22
end: 273
orientation: inline

URA3 (uracil selection marker)
type: SequenceAnnotation
persistent identity: annotation2516563
display id: annotation2516563
version: 1
top level: pSB1A8YL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516563/range2516563/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516563/range2516563/1
type: Range
persistent identity: range2516563
display id: range2516563
version: 1
direction: direction/reverse
top level: pSB1A8YL
start: 595
end: 1455
orientation: inline

Cen1-1 origin of replication
type: SequenceAnnotation
persistent identity: annotation2516562
display id: annotation2516562
version: 1
top level: pSB1A8YL
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516562/range2516562/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516562/range2516562/1
type: Range
persistent identity: range2516562
display id: range2516562
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 327
end: 427
orientation: inline

C to G
type: SequenceAnnotation
persistent identity: annotation2516568
display id: annotation2516568
version: 1
top level: pSB1A8YL
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K2117009/annotation2516568/range2516568/1

https://synbiohub.org/public/igem/BBa_K2117009/annotation2516568/range2516568/1
type: Range
persistent identity: range2516568
display id: range2516568
version: 1
direction: direction/forward
top level: pSB1A8YL
start: 1496
end: 1496
orientation: inline

BBa_K516999
type: ComponentDefinition
persistent identity: BBa K516999
display id: BBa_K516999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3 with RBS-RFP-TT between SpeI-PstI
creator: Nicolo' Politi, Susanna Zucca, Tommaso Goggia, Paolo Magni
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: When the SpeI-PstI DNA fragment from BBa_J61002 , containing the mRFP1 gene with a strong RBS and a double terminator, is transferred into pSB1C3 (digested SpeI-PstI) the resulting plasmid is BBa_K516999. See pSB1C3 for detailed feature information.
mutable notes: This is not a standard BioBrick plasmid, as the suffix is not SpeI-NotI-PstI.
mutable provenance: pSB1C3 (backbone) and BBa_J61002 (SpeI-PstI insert).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K516999_sequence/1

BBa_K731710
type: ComponentDefinition
persistent identity: BBa K731710
display id: BBa_K731710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Platform for terminators analysis under the control of tac promoter
creator: Giacomo Giacomelli, Anna Depetris
created: 2012-08-14T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12063
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the twin construct of BBa_K731700, with the same two subsequent fluorescent proteins, with T7 promoter replaced with Ptac. This construct allows to test possible differences in terminator efficiency due to different promoter and thus polymerase. We tested both constructs in the E.Coli strain BL21pLysS, that has both polymerases.
mutable notes: The presence of the promoter cannot be tested with screening digestion, as it has more or less the same T7 promoter`s length. Anyway the screening in amplification strains is much more easy: the right colonies will be intensely red.
mutable provenance: The Ptac promoter has been inserted with a PCR, the sequence of the promoter has been taken from literature, while the original plamid is pET21b
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: A206K VENUS
sequence annotation: lacI
sequence annotation: mCHERRY
sequence annotation: T7 TERMINATOR
sequence annotation: ampicillin resistance gene
sequence annotation: lacO
sequence annotation: Ptac
sequence: https://synbiohub.org/public/igem/BBa_K731710_sequence/1

A206K VENUS
type: SequenceAnnotation
persistent identity: annotation2179555
display id: annotation2179555
version: 1
top level: BBa_K731710
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731710/annotation2179555/range2179555/1

https://synbiohub.org/public/igem/BBa_K731710/annotation2179555/range2179555/1
type: Range
persistent identity: range2179555
display id: range2179555
version: 1
direction: direction/forward
top level: BBa_K731710
start: 36
end: 752
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2179565
display id: annotation2179565
version: 1
top level: BBa_K731710
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731710/annotation2179565/range2179565/1

https://synbiohub.org/public/igem/BBa_K731710/annotation2179565/range2179565/1
type: Range
persistent identity: range2179565
display id: range2179565
version: 1
direction: direction/reverse
top level: BBa_K731710
start: 4561
end: 5653
orientation: inline

mCHERRY
type: SequenceAnnotation
persistent identity: annotation2179568
display id: annotation2179568
version: 1
top level: BBa_K731710
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731710/annotation2179568/range2179568/1

https://synbiohub.org/public/igem/BBa_K731710/annotation2179568/range2179568/1
type: Range
persistent identity: range2179568
display id: range2179568
version: 1
direction: direction/forward
top level: BBa_K731710
start: 6134
end: 6844
orientation: inline

T7 TERMINATOR
type: SequenceAnnotation
persistent identity: annotation2179563
display id: annotation2179563
version: 1
top level: BBa_K731710
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K731710/annotation2179563/range2179563/1

https://synbiohub.org/public/igem/BBa_K731710/annotation2179563/range2179563/1
type: Range
persistent identity: range2179563
display id: range2179563
version: 1
direction: direction/forward
top level: BBa_K731710
start: 843
end: 890
orientation: inline

ampicillin resistance gene
type: SequenceAnnotation
persistent identity: annotation2179564
display id: annotation2179564
version: 1
top level: BBa_K731710
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731710/annotation2179564/range2179564/1

https://synbiohub.org/public/igem/BBa_K731710/annotation2179564/range2179564/1
type: Range
persistent identity: range2179564
display id: range2179564
version: 1
direction: direction/forward
top level: BBa_K731710
start: 1711
end: 2370
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation2179567
display id: annotation2179567
version: 1
top level: BBa_K731710
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K731710/annotation2179567/range2179567/1

https://synbiohub.org/public/igem/BBa_K731710/annotation2179567/range2179567/1
type: Range
persistent identity: range2179567
display id: range2179567
version: 1
direction: direction/forward
top level: BBa_K731710
start: 6065
end: 6087
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2179566
display id: annotation2179566
version: 1
top level: BBa_K731710
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731710/annotation2179566/range2179566/1

https://synbiohub.org/public/igem/BBa_K731710/annotation2179566/range2179566/1
type: Range
persistent identity: range2179566
display id: range2179566
version: 1
direction: direction/forward
top level: BBa_K731710
start: 6033
end: 6061
orientation: inline

pSB4C50
type: ComponentDefinition
persistent identity: BBa K1362096
display id: BBa_K1362096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4C50: Low(?) copy BioBrick cloning/expression backbone carrying Cm resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_P1004
component: BBa_G00001
component: BBa_B0055
component: BBa_B0043
component: BBa_G0004
component: BBa_G0003
component: BBa_G00102
component: BBa_G0002
component: BBa_B0044
component: BBa_B0062
component: BBa_B0042
component: BBa_B0053
component: BBa_K1362463
component: BBa_B0054
component: BBa_I50042
component: BBa_G00100
component: BBa_G00000
sequence annotation: BBa_G00102
sequence annotation: BBa_G0004
sequence annotation: BBa_P1004
sequence annotation: BBa_B0042
sequence annotation: BBa_B0043
sequence annotation: BBa_I50042
sequence annotation: BBa_G00001
sequence annotation: BBa_B0062
sequence annotation: BBa_G0003
sequence annotation: BBa_G0002
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_G00000
sequence annotation: BBa_K1362463
sequence annotation: BBa_B0054
sequence annotation: BBa_B0055
sequence annotation: BBa_G00100
sequence: https://synbiohub.org/public/igem/BBa_K1362096_sequence/1

BBa_P1004
type: Component
persistent identity: component2409903
display id: component2409903
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_G00001
type: Component
persistent identity: component2409852
display id: component2409852
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0055
type: Component
persistent identity: component2409911
display id: component2409911
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0043
type: Component
persistent identity: component2409923
display id: component2409923
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G0004
type: Component
persistent identity: component2409904
display id: component2409904
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G0003
type: Component
persistent identity: component2409872
display id: component2409872
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00102
type: Component
persistent identity: component2409869
display id: component2409869
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G0002
type: Component
persistent identity: component2409901
display id: component2409901
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0044
type: Component
persistent identity: component2409857
display id: component2409857
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409871
display id: component2409871
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0042
type: Component
persistent identity: component2409864
display id: component2409864
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0042
type: Component
persistent identity: component2409918
display id: component2409918
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0053
type: Component
persistent identity: component2409906
display id: component2409906
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_K1362463
type: Component
persistent identity: component2409927
display id: component2409927
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_B0054
type: Component
persistent identity: component2409867
display id: component2409867
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_I50042
type: Component
persistent identity: component2409900
display id: component2409900
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_G00100
type: Component
persistent identity: component2409908
display id: component2409908
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00000
type: Component
persistent identity: component2409932
display id: component2409932
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409869
display id: annotation2409869
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409869/range2409869/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409869/range2409869/1
type: Range
persistent identity: range2409869
display id: range2409869
version: 1
direction: direction/reverse
top level: pSB4C50
start: 148
end: 167
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409904
display id: annotation2409904
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
type: Range
persistent identity: range2409904
display id: range2409904
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3078
end: 3084
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2409903
display id: annotation2409903
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
component: BBa_P1004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
type: Range
persistent identity: range2409903
display id: range2409903
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2301
end: 3069
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409918
display id: annotation2409918
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409918/range2409918/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409918/range2409918/1
type: Range
persistent identity: range2409918
display id: range2409918
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3287
end: 3298
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409923
display id: annotation2409923
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
type: Range
persistent identity: range2409923
display id: range2409923
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3307
end: 3319
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409900
display id: annotation2409900
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409900/range2409900/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409900/range2409900/1
type: Range
persistent identity: range2409900
display id: range2409900
version: 1
direction: direction/reverse
top level: pSB4C50
start: 240
end: 2276
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409852
display id: annotation2409852
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409852/range2409852/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409852/range2409852/1
type: Range
persistent identity: range2409852
display id: range2409852
version: 1
direction: direction/reverse
top level: pSB4C50
start: 1
end: 21
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409871
display id: annotation2409871
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
type: Range
persistent identity: range2409871
display id: range2409871
version: 1
direction: direction/reverse
top level: pSB4C50
start: 176
end: 216
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409872
display id: annotation2409872
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409872/range2409872/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409872/range2409872/1
type: Range
persistent identity: range2409872
display id: range2409872
version: 1
direction: direction/reverse
top level: pSB4C50
start: 225
end: 231
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409901
display id: annotation2409901
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
type: Range
persistent identity: range2409901
display id: range2409901
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2285
end: 2292
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409906
display id: annotation2409906
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
type: Range
persistent identity: range2409906
display id: range2409906
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3093
end: 3164
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409864
display id: annotation2409864
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409864/range2409864/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409864/range2409864/1
type: Range
persistent identity: range2409864
display id: range2409864
version: 1
direction: direction/reverse
top level: pSB4C50
start: 51
end: 62
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409857
display id: annotation2409857
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409857/range2409857/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409857/range2409857/1
type: Range
persistent identity: range2409857
display id: range2409857
version: 1
direction: direction/reverse
top level: pSB4C50
start: 30
end: 42
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409932
display id: annotation2409932
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409932/range2409932/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409932/range2409932/1
type: Range
persistent identity: range2409932
display id: range2409932
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3387
end: 3408
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409927
display id: annotation2409927
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
type: Range
persistent identity: range2409927
display id: range2409927
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3328
end: 3378
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409867
display id: annotation2409867
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409867/range2409867/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409867/range2409867/1
type: Range
persistent identity: range2409867
display id: range2409867
version: 1
direction: direction/reverse
top level: pSB4C50
start: 71
end: 139
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409911
display id: annotation2409911
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409911/range2409911/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409911/range2409911/1
type: Range
persistent identity: range2409911
display id: range2409911
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3201
end: 3278
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409908
display id: annotation2409908
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409908/range2409908/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409908/range2409908/1
type: Range
persistent identity: range2409908
display id: range2409908
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3173
end: 3192
orientation: inline

BBa_K1444027
type: ComponentDefinition
persistent identity: BBa K1444027
display id: BBa_K1444027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pUC57 cloning vector from BioBasic
creator: Dave Curran
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1822_
m user id: 0
owner id: 1972
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Descr
mutable notes: Desgin
mutable provenance: source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ampR
sequence annotation: Cloning site
sequence annotation: LacZ alpha
sequence annotation: ColE1 replication origin
sequence: https://synbiohub.org/public/igem/BBa_K1444027_sequence/1

ampR
type: SequenceAnnotation
persistent identity: annotation2427437
display id: annotation2427437
version: 1
top level: BBa_K1444027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1444027/annotation2427437/range2427437/1

https://synbiohub.org/public/igem/BBa_K1444027/annotation2427437/range2427437/1
type: Range
persistent identity: range2427437
display id: range2427437
version: 1
direction: direction/reverse
top level: BBa_K1444027
start: 1590
end: 2249
orientation: inline

Cloning site
type: SequenceAnnotation
persistent identity: annotation2427435
display id: annotation2427435
version: 1
top level: BBa_K1444027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1444027/annotation2427435/range2427435/1

https://synbiohub.org/public/igem/BBa_K1444027/annotation2427435/range2427435/1
type: Range
persistent identity: range2427435
display id: range2427435
version: 1
direction: direction/forward
top level: BBa_K1444027
start: 396
end: 413
orientation: inline

LacZ alpha
type: SequenceAnnotation
persistent identity: annotation2427434
display id: annotation2427434
version: 1
top level: BBa_K1444027
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1444027/annotation2427434/range2427434/1

https://synbiohub.org/public/igem/BBa_K1444027/annotation2427434/range2427434/1
type: Range
persistent identity: range2427434
display id: range2427434
version: 1
direction: direction/reverse
top level: BBa_K1444027
start: 146
end: 430
orientation: inline

ColE1 replication origin
type: SequenceAnnotation
persistent identity: annotation2427436
display id: annotation2427436
version: 1
top level: BBa_K1444027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1444027/annotation2427436/range2427436/1

https://synbiohub.org/public/igem/BBa_K1444027/annotation2427436/range2427436/1
type: Range
persistent identity: range2427436
display id: range2427436
version: 1
direction: direction/forward
top level: BBa_K1444027
start: 810
end: 1492
orientation: inline

BAC
type: ComponentDefinition
persistent identity: BBa J61031
display id: BBa_J61031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Amplifiable BAC plasmid
creator: John Anderson
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This plasmid is derived originally from pBeloBAC11 with multiple modifications to make it compatible with biobricks and more useable. Without modification, J61031 is a high-copy plasmid in normal laboratory strains of E. coli due to the colE1 origin of replication located within the biobrick part region. It also confers ampicillin resistance, kanamycin resistance, and constitutively-expressed RFP. Upon replacement of the SpeI/XbaI region, the colE1, ampR, and RFP features are removed, leaving behind the F plasmid replicon, the KanR gene, and an R6K origin of replication. At this point, the plasmid will replicate as a medium or high copy plasmid in pir+ or pir116 strains such as Ec100D::pir116. In non-pir strains (most lab strains), the plasmid will replicate at near-single copy levels. The restriction sites EcoRI, SpeI, NotI, and XbaI are all unique in this plasmid. There are several PstI sites, however. pBACr-NESd
mutable notes: N/A
mutable provenance: many many steps
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: finO incD/sopC region; contains 12 43 bp direct repeats that bind SopB protein; required for partitioning of F plasmid to daughter cells after replication as well as repression of sopAB operator;
sequence annotation: pBR322 origin
sequence annotation: kanamycin/neomycin resistance marker (NTP II marker)
sequence annotation: PstI
sequence annotation: SpeI
sequence annotation: GFP reporter
sequence annotation: beta-lactamase ampicillin resistance marker
sequence annotation: c myc tag
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: pMB1 replication origin
sequence annotation: NotI
sequence annotation: sopB
sequence annotation: R6Kg origin
sequence annotation: kanR/neoR promoter
sequence annotation: rrnB T1 terminator
sequence annotation: mRFP
sequence annotation: EcoRI
sequence annotation: BBa_R0040 Ptet promoter
sequence annotation: 6xHis tag
sequence annotation: sopA
sequence annotation: repE
sequence annotation: rrnB terminator
sequence: https://synbiohub.org/public/igem/BBa_J61031_sequence/1

finO incD/sopC region; contains 12 43 bp direct repeats that bind SopB protein; required for partitioning of F plasmid to daughter cells after replication as well as repression of sopAB operator;
type: SequenceAnnotation
persistent identity: annotation1976020
display id: annotation1976020
version: 1
top level: BAC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976020/range1976020/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976020/range1976020/1
type: Range
persistent identity: range1976020
display id: range1976020
version: 1
direction: direction/reverse
top level: BAC
start: 145
end: 618
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1934582
display id: annotation1934582
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1934582/range1934582/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1934582/range1934582/1
type: Range
persistent identity: range1934582
display id: range1934582
version: 1
direction: direction/forward
top level: BAC
start: 7864
end: 8303
orientation: inline

kanamycin/neomycin resistance marker (NTP II marker)
type: SequenceAnnotation
persistent identity: annotation1934584
display id: annotation1934584
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1934584/range1934584/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1934584/range1934584/1
type: Range
persistent identity: range1934584
display id: range1934584
version: 1
direction: direction/forward
top level: BAC
start: 4891
end: 5685
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976016
display id: annotation1976016
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976016/range1976016/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976016/range1976016/1
type: Range
persistent identity: range1976016
display id: range1976016
version: 1
direction: direction/forward
top level: BAC
start: 5068
end: 5073
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1976012
display id: annotation1976012
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976012/range1976012/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976012/range1976012/1
type: Range
persistent identity: range1976012
display id: range1976012
version: 1
direction: direction/forward
top level: BAC
start: 2
end: 7
orientation: inline

GFP reporter
type: SequenceAnnotation
persistent identity: annotation1976009
display id: annotation1976009
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976009/range1976009/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976009/range1976009/1
type: Range
persistent identity: range1976009
display id: range1976009
version: 1
direction: direction/reverse
top level: BAC
start: 8985
end: 9698
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1976008
display id: annotation1976008
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976008/range1976008/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976008/range1976008/1
type: Range
persistent identity: range1976008
display id: range1976008
version: 1
direction: direction/forward
top level: BAC
start: 6669
end: 7529
orientation: inline

c myc tag
type: SequenceAnnotation
persistent identity: annotation1976006
display id: annotation1976006
version: 1
top level: BAC
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976006/range1976006/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976006/range1976006/1
type: Range
persistent identity: range1976006
display id: range1976006
version: 1
direction: direction/reverse
top level: BAC
start: 6484
end: 6513
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1976007
display id: annotation1976007
version: 1
top level: BAC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976007/range1976007/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976007/range1976007/1
type: Range
persistent identity: range1976007
display id: range1976007
version: 1
direction: direction/forward
top level: BAC
start: 6599
end: 6627
orientation: inline

pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation1934583
display id: annotation1934583
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1934583/range1934583/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1934583/range1934583/1
type: Range
persistent identity: range1934583
display id: range1934583
version: 1
direction: direction/reverse
top level: BAC
start: 7691
end: 8303
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1976013
display id: annotation1976013
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976013/range1976013/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976013/range1976013/1
type: Range
persistent identity: range1976013
display id: range1976013
version: 1
direction: direction/forward
top level: BAC
start: 9
end: 16
orientation: inline

sopB
type: SequenceAnnotation
persistent identity: annotation1975998
display id: annotation1975998
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1975998/range1975998/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1975998/range1975998/1
type: Range
persistent identity: range1975998
display id: range1975998
version: 1
direction: direction/reverse
top level: BAC
start: 691
end: 1662
orientation: inline

R6Kg origin
type: SequenceAnnotation
persistent identity: annotation1976002
display id: annotation1976002
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976002/range1976002/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976002/range1976002/1
type: Range
persistent identity: range1976002
display id: range1976002
version: 1
direction: direction/reverse
top level: BAC
start: 5743
end: 6126
orientation: inline

kanR/neoR promoter
type: SequenceAnnotation
persistent identity: annotation1976001
display id: annotation1976001
version: 1
top level: BAC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976001/range1976001/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976001/range1976001/1
type: Range
persistent identity: range1976001
display id: range1976001
version: 1
direction: direction/forward
top level: BAC
start: 4753
end: 4802
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1976003
display id: annotation1976003
version: 1
top level: BAC
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976003/range1976003/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976003/range1976003/1
type: Range
persistent identity: range1976003
display id: range1976003
version: 1
direction: direction/reverse
top level: BAC
start: 6179
end: 6222
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976018
display id: annotation1976018
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976018/range1976018/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976018/range1976018/1
type: Range
persistent identity: range1976018
display id: range1976018
version: 1
direction: direction/forward
top level: BAC
start: 8975
end: 8980
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1976011
display id: annotation1976011
version: 1
top level: BAC
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976011/range1976011/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976011/range1976011/1
type: Range
persistent identity: range1976011
display id: range1976011
version: 1
direction: direction/forward
top level: BAC
start: 10540
end: 10583
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976014
display id: annotation1976014
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976014/range1976014/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976014/range1976014/1
type: Range
persistent identity: range1976014
display id: range1976014
version: 1
direction: direction/forward
top level: BAC
start: 2001
end: 2006
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation1976010
display id: annotation1976010
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976010/range1976010/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976010/range1976010/1
type: Range
persistent identity: range1976010
display id: range1976010
version: 1
direction: direction/forward
top level: BAC
start: 9815
end: 10495
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1976017
display id: annotation1976017
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976017/range1976017/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976017/range1976017/1
type: Range
persistent identity: range1976017
display id: range1976017
version: 1
direction: direction/forward
top level: BAC
start: 6527
end: 6532
orientation: inline

BBa_R0040 Ptet promoter
type: SequenceAnnotation
persistent identity: annotation1976021
display id: annotation1976021
version: 1
top level: BAC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976021/range1976021/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976021/range1976021/1
type: Range
persistent identity: range1976021
display id: range1976021
version: 1
direction: direction/forward
top level: BAC
start: 9735
end: 9788
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976015
display id: annotation1976015
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976015/range1976015/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976015/range1976015/1
type: Range
persistent identity: range1976015
display id: range1976015
version: 1
direction: direction/forward
top level: BAC
start: 3542
end: 3547
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation1976005
display id: annotation1976005
version: 1
top level: BAC
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976005/range1976005/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976005/range1976005/1
type: Range
persistent identity: range1976005
display id: range1976005
version: 1
direction: direction/reverse
top level: BAC
start: 6451
end: 6468
orientation: inline

sopA
type: SequenceAnnotation
persistent identity: annotation1975999
display id: annotation1975999
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1975999/range1975999/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1975999/range1975999/1
type: Range
persistent identity: range1975999
display id: range1975999
version: 1
direction: direction/reverse
top level: BAC
start: 1662
end: 2837
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1976000
display id: annotation1976000
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976000/range1976000/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976000/range1976000/1
type: Range
persistent identity: range1976000
display id: range1976000
version: 1
direction: direction/reverse
top level: BAC
start: 3416
end: 4171
orientation: inline

rrnB terminator
type: SequenceAnnotation
persistent identity: annotation1976004
display id: annotation1976004
version: 1
top level: BAC
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976004/range1976004/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976004/range1976004/1
type: Range
persistent identity: range1976004
display id: range1976004
version: 1
direction: direction/reverse
top level: BAC
start: 6188
end: 6345
orientation: inline

pSBBs2E
type: ComponentDefinition
persistent identity: BBa K823027
display id: BBa_K823027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs2E: Empty backbone for integration into Bacillus subtilis lacA locus
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli. This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection in E.coli.
mutable provenance: This part is an igem compatible version of the Bacillus subtilis vector pAX01
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: erm
sequence annotation: bla
sequence annotation: lacA'
sequence annotation: 'lacA
sequence: https://synbiohub.org/public/igem/BBa_K823027_sequence/1

erm
type: SequenceAnnotation
persistent identity: annotation2180263
display id: annotation2180263
version: 1
top level: pSBBs2E
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180263/range2180263/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180263/range2180263/1
type: Range
persistent identity: range2180263
display id: range2180263
version: 1
direction: direction/forward
top level: pSBBs2E
start: 500
end: 1300
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180265
display id: annotation2180265
version: 1
top level: pSBBs2E
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180265/range2180265/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180265/range2180265/1
type: Range
persistent identity: range2180265
display id: range2180265
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 4603
end: 5400
orientation: inline

lacA'
type: SequenceAnnotation
persistent identity: annotation2180264
display id: annotation2180264
version: 1
top level: pSBBs2E
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180264/range2180264/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180264/range2180264/1
type: Range
persistent identity: range2180264
display id: range2180264
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 1600
end: 2100
orientation: inline

'lacA
type: SequenceAnnotation
persistent identity: annotation2180266
display id: annotation2180266
version: 1
top level: pSBBs2E
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180266/range2180266/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180266/range2180266/1
type: Range
persistent identity: range2180266
display id: range2180266
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 5600
end: 6100
orientation: inline

pSB3K5
type: ComponentDefinition
persistent identity: pSB3K5
display id: pSB3K5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-05-02T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3K5 is a low to medium copy plasmid based with kanamycin resistance. It has a p15A-derived replication origin. pSB3K5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the kanamycin resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00100
component: BBa_G0004
component: BBa_B0055
component: BBa_G0003
component: BBa_B0043
component: BBa_P1003
component: BBa_B0044
component: BBa_B0042
component: BBa_G0002
component: BBa_B0062
component: BBa_G00102
component: BBa_B0054
component: BBa_G00001
component: BBa_I50032
component: BBa_G00000
component: BBa_B0053
sequence annotation: BBa_B0042
sequence annotation: BBa_G00000
sequence annotation: BBa_B0062
sequence annotation: BBa_B0044
sequence annotation: BBa_B0054
sequence annotation: BBa_G00100
sequence annotation: BBa_B0053
sequence annotation: BBa_G00001
sequence annotation: BBa_G0002
sequence annotation: BBa_B0055
sequence annotation: BBa_G0003
sequence annotation: BBa_P1003
sequence annotation: BBa_I50032
sequence annotation: BBa_B0043
sequence annotation: BBa_G0004
sequence annotation: BBa_G00102
sequence: https://synbiohub.org/public/igem/pSB3K5_sequence/1

BBa_G00100
type: Component
persistent identity: component1932107
display id: component1932107
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G0004
type: Component
persistent identity: component1932104
display id: component1932104
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0055
type: Component
persistent identity: component1932110
display id: component1932110
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G0003
type: Component
persistent identity: component1932097
display id: component1932097
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0043
type: Component
persistent identity: component1932122
display id: component1932122
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_P1003
type: Component
persistent identity: component1932103
display id: component1932103
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1003/1

BBa_B0044
type: Component
persistent identity: component1932083
display id: component1932083
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0042
type: Component
persistent identity: component1932090
display id: component1932090
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0002
type: Component
persistent identity: component1932101
display id: component1932101
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0062
type: Component
persistent identity: component1932096
display id: component1932096
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G00102
type: Component
persistent identity: component1932094
display id: component1932094
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component1932117
display id: component1932117
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0054
type: Component
persistent identity: component1932093
display id: component1932093
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00001
type: Component
persistent identity: component1932078
display id: component1932078
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_I50032
type: Component
persistent identity: component1932100
display id: component1932100
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_G00000
type: Component
persistent identity: component1932124
display id: component1932124
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0053
type: Component
persistent identity: component1932106
display id: component1932106
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1932117
display id: annotation1932117
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932117/range1932117/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3K5/annotation1932117/range1932117/1
type: Range
persistent identity: range1932117
display id: range1932117
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2890
end: 2901
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1932124
display id: annotation1932124
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932124/range1932124/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3K5/annotation1932124/range1932124/1
type: Range
persistent identity: range1932124
display id: range1932124
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2915
end: 2936
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1932096
display id: annotation1932096
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932096/range1932096/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3K5/annotation1932096/range1932096/1
type: Range
persistent identity: range1932096
display id: range1932096
version: 1
direction: direction/reverse
top level: pSB3K5
start: 136
end: 176
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1932083
display id: annotation1932083
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932083/range1932083/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3K5/annotation1932083/range1932083/1
type: Range
persistent identity: range1932083
display id: range1932083
version: 1
direction: direction/reverse
top level: pSB3K5
start: 22
end: 34
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1932093
display id: annotation1932093
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932093/range1932093/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3K5/annotation1932093/range1932093/1
type: Range
persistent identity: range1932093
display id: range1932093
version: 1
direction: direction/reverse
top level: pSB3K5
start: 47
end: 115
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1932107
display id: annotation1932107
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932107/range1932107/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3K5/annotation1932107/range1932107/1
type: Range
persistent identity: range1932107
display id: range1932107
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2792
end: 2811
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1932090
display id: annotation1932090
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932090/range1932090/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3K5/annotation1932090/range1932090/1
type: Range
persistent identity: range1932090
display id: range1932090
version: 1
direction: direction/reverse
top level: pSB3K5
start: 35
end: 46
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1932106
display id: annotation1932106
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932106/range1932106/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3K5/annotation1932106/range1932106/1
type: Range
persistent identity: range1932106
display id: range1932106
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2720
end: 2791
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1932078
display id: annotation1932078
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932078/range1932078/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3K5/annotation1932078/range1932078/1
type: Range
persistent identity: range1932078
display id: range1932078
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1
end: 21
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1932101
display id: annotation1932101
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932101/range1932101/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3K5/annotation1932101/range1932101/1
type: Range
persistent identity: range1932101
display id: range1932101
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1738
end: 1745
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1932110
display id: annotation1932110
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932110/range1932110/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3K5/annotation1932110/range1932110/1
type: Range
persistent identity: range1932110
display id: range1932110
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2812
end: 2889
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1932097
display id: annotation1932097
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932097/range1932097/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3K5/annotation1932097/range1932097/1
type: Range
persistent identity: range1932097
display id: range1932097
version: 1
direction: direction/reverse
top level: pSB3K5
start: 177
end: 183
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation1932103
display id: annotation1932103
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932103/range1932103/1
component: BBa_P1003

https://synbiohub.org/public/igem/pSB3K5/annotation1932103/range1932103/1
type: Range
persistent identity: range1932103
display id: range1932103
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1746
end: 2712
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1932100
display id: annotation1932100
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932100/range1932100/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3K5/annotation1932100/range1932100/1
type: Range
persistent identity: range1932100
display id: range1932100
version: 1
direction: direction/reverse
top level: pSB3K5
start: 184
end: 1737
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1932122
display id: annotation1932122
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932122/range1932122/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3K5/annotation1932122/range1932122/1
type: Range
persistent identity: range1932122
display id: range1932122
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2902
end: 2914
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1932104
display id: annotation1932104
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932104/range1932104/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3K5/annotation1932104/range1932104/1
type: Range
persistent identity: range1932104
display id: range1932104
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2713
end: 2719
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1932094
display id: annotation1932094
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932094/range1932094/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3K5/annotation1932094/range1932094/1
type: Range
persistent identity: range1932094
display id: range1932094
version: 1
direction: direction/reverse
top level: pSB3K5
start: 116
end: 135
orientation: inline

BBa_K1480014
type: ComponentDefinition
persistent identity: BBa K1480014
display id: BBa_K1480014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB3C5 + After TALEN (K1480001) Target site
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Backbone vector of pSB3C5 that contains a single modification with a TALEN target site after the origin of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains a single TALEN target site (AF).
mutable notes: Modifications where inserted by PCR on both directions due to TALEN specificity.
mutable provenance: Derived from pSB3C5 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1480014_sequence/1

BBa_K1362092
type: ComponentDefinition
persistent identity: BBa K1362092
display id: BBa_K1362092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C30: High copy BioBrick cloning/expression backbone carrying Cm resitance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Was archived by cloning an oligo containing the promoter into the EcoRI site of pSB1C3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: T0 terminator
sequence annotation: VR
sequence annotation: VF2
sequence annotation: rep(pMB1) (rev)
sequence annotation: terminator stem loop
sequence annotation: ORI
sequence annotation: T7 promotor
sequence annotation: end of terminator
sequence annotation: Chloramphenicol resistance
sequence annotation: prefix
sequence annotation: lacO reg
sequence annotation: suffix
sequence annotation: E. coli his operon terminator
sequence: https://synbiohub.org/public/igem/BBa_K1362092_sequence/1

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2405055
display id: annotation2405055
version: 1
top level: BBa_K1362092
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405055/range2405055/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405055/range2405055/1
type: Range
persistent identity: range2405055
display id: range2405055
version: 1
direction: direction/forward
top level: BBa_K1362092
start: 1042
end: 1147
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2405045
display id: annotation2405045
version: 1
top level: BBa_K1362092
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405045/range2405045/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405045/range2405045/1
type: Range
persistent identity: range2405045
display id: range2405045
version: 1
direction: direction/reverse
top level: BBa_K1362092
start: 157
end: 176
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2405049
display id: annotation2405049
version: 1
top level: BBa_K1362092
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405049/range2405049/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405049/range2405049/1
type: Range
persistent identity: range2405049
display id: range2405049
version: 1
direction: direction/forward
top level: BBa_K1362092
start: 1933
end: 1952
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2405046
display id: annotation2405046
version: 1
top level: BBa_K1362092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405046/range2405046/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405046/range2405046/1
type: Range
persistent identity: range2405046
display id: range2405046
version: 1
direction: direction/reverse
top level: BBa_K1362092
start: 261
end: 875
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2405044
display id: annotation2405044
version: 1
top level: BBa_K1362092
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405044/range2405044/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405044/range2405044/1
type: Range
persistent identity: range2405044
display id: range2405044
version: 1
direction: direction/forward
top level: BBa_K1362092
start: 30
end: 64
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2405047
display id: annotation2405047
version: 1
top level: BBa_K1362092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405047/range2405047/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405047/range2405047/1
type: Range
persistent identity: range2405047
display id: range2405047
version: 1
direction: direction/forward
top level: BBa_K1362092
start: 276
end: 276
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2405052
display id: annotation2405052
version: 1
top level: BBa_K1362092
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405052/range2405052/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405052/range2405052/1
type: Range
persistent identity: range2405052
display id: range2405052
version: 1
direction: direction/forward
top level: BBa_K1362092
start: 2054
end: 2072
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2405050
display id: annotation2405050
version: 1
top level: BBa_K1362092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405050/range2405050/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405050/range2405050/1
type: Range
persistent identity: range2405050
display id: range2405050
version: 1
direction: direction/reverse
top level: BBa_K1362092
start: 2009
end: 2012
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2405048
display id: annotation2405048
version: 1
top level: BBa_K1362092
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405048/range2405048/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405048/range2405048/1
type: Range
persistent identity: range2405048
display id: range2405048
version: 1
direction: direction/reverse
top level: BBa_K1362092
start: 1160
end: 1819
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2405054
display id: annotation2405054
version: 1
top level: BBa_K1362092
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405054/range2405054/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405054/range2405054/1
type: Range
persistent identity: range2405054
display id: range2405054
version: 1
direction: direction/forward
top level: BBa_K1362092
start: 2100
end: 2121
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2405051
display id: annotation2405051
version: 1
top level: BBa_K1362092
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405051/range2405051/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405051/range2405051/1
type: Range
persistent identity: range2405051
display id: range2405051
version: 1
direction: direction/reverse
top level: BBa_K1362092
start: 2022
end: 2041
orientation: inline

lacO reg
type: SequenceAnnotation
persistent identity: annotation2405053
display id: annotation2405053
version: 1
top level: BBa_K1362092
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405053/range2405053/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405053/range2405053/1
type: Range
persistent identity: range2405053
display id: range2405053
version: 1
direction: direction/forward
top level: BBa_K1362092
start: 2072
end: 2099
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2405042
display id: annotation2405042
version: 1
top level: BBa_K1362092
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405042/range2405042/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405042/range2405042/1
type: Range
persistent identity: range2405042
display id: range2405042
version: 1
direction: direction/forward
top level: BBa_K1362092
start: 1
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2405043
display id: annotation2405043
version: 1
top level: BBa_K1362092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362092/annotation2405043/range2405043/1

https://synbiohub.org/public/igem/BBa_K1362092/annotation2405043/range2405043/1
type: Range
persistent identity: range2405043
display id: range2405043
version: 1
direction: direction/forward
top level: BBa_K1362092
start: 22
end: 93
orientation: inline

BBa_K1256000
type: ComponentDefinition
persistent identity: BBa K1256000
display id: BBa_K1256000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEST
creator: Pei-Hong Chen
created: 2014-05-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1614_
m user id: 0
owner id: 8995
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: TEST
mutable notes: TEST
mutable provenance: TEST
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

pSac-Cm_B
type: ComponentDefinition
persistent identity: BBa K1185004
display id: BBa_K1185004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Integration vector for Bacillus subtilis derived from pSac-Cm
creator: Vincent Leonardo
created: 2013-10-03T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1498_
m user id: 0
owner id: 16794
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This plasmid was derived from pSac-Cm by insertion of biobrick compatible restriction sites (prefixes and suffixes), a terminator (BBa_B0015) after the suffixes sequences, and red fluorescent protein sequence (RFP) in between the prefix and suffix in its multiple cloning sites (MCS). New biobricks can be inserted into this vector by replacement of the RFP biobrick, and selection of the white colonies. This backbone has a multi host replication origin and replicates in E. coli. The plasmid is designed to integrate a cloned insert into the B. subtilis chromosome via double recombination between plasmid and chromosomal sacA sequences, and in order to check for integration a simple Phenol Red Sucrose test can be perform, if sacA is no longer functional, they will remain ph7, if they are still able to digest sucrose their pH will be under 5.
mutable notes: We found that that the integration plasmid that groningen send to the registry were unable to integrate in B. subtilis. This is due to the high number of mutations in the sacA integration region.
mutable provenance: Groningen iGEM 2012 team
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 'sacA
sequence annotation: cat
sequence annotation: B0015
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_K1185004_sequence/1

'sacA
type: SequenceAnnotation
persistent identity: annotation2367616
display id: annotation2367616
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367616/range2367616/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367616/range2367616/1
type: Range
persistent identity: range2367616
display id: range2367616
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 3167
end: 3922
orientation: inline

'sacA
type: SequenceAnnotation
persistent identity: annotation2367614
display id: annotation2367614
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367614/range2367614/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367614/range2367614/1
type: Range
persistent identity: range2367614
display id: range2367614
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 167
end: 970
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2367617
display id: annotation2367617
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367617/range2367617/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367617/range2367617/1
type: Range
persistent identity: range2367617
display id: range2367617
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 4231
end: 4894
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2367613
display id: annotation2367613
version: 1
top level: pSac-Cm_B
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367613/range2367613/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367613/range2367613/1
type: Range
persistent identity: range2367613
display id: range2367613
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 23
end: 151
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2367615
display id: annotation2367615
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367615/range2367615/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367615/range2367615/1
type: Range
persistent identity: range2367615
display id: range2367615
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 1306
end: 2176
orientation: inline

BBa_K797016
type: ComponentDefinition
persistent identity: BBa K797016
display id: BBa_K797016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid Backbone including M-13 primer region
creator: Komoto Yusuke
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1053_
m user id: 0
owner id: 13855
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid Backbone that contains M13-fwd
mutable provenance: This plasmid was ssent from a company that assists iGEM.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BBa_K797016
sequence: https://synbiohub.org/public/igem/BBa_K797016_sequence/1

BBa_K797016
type: SequenceAnnotation
persistent identity: annotation2201538
display id: annotation2201538
version: 1
top level: BBa_K797016
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K797016/annotation2201538/range2201538/1

https://synbiohub.org/public/igem/BBa_K797016/annotation2201538/range2201538/1
type: Range
persistent identity: range2201538
display id: range2201538
version: 1
direction: direction/reverse
top level: BBa_K797016
start: 1
end: 2041
orientation: inline

BBa_K1971005
type: ComponentDefinition
persistent identity: BBa K1971005
display id: BBa_K1971005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pet28a backbone with BsaI site
creator: KE GUAN YU
created: 2016-10-19T11:00:00Z
modified: 2016-10-20T02:14:18Z
discontinued: false
dominant: false
group u list: _2438_
m user id: 29894
owner id: 29894
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pet28a backbone with BsaI site
mutable notes: none
mutable provenance: Pet28a plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1971005_sequence/1

BBa_K911010
type: ComponentDefinition
persistent identity: BBa K911010
display id: BBa_K911010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacillus shuttle vector (PJS130)
creator: Thomas Whittaker
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
group u list: _1176_
m user id: 0
owner id: 12683
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Bacillus shuttle vector used for assembling our fluorescent ratiometric construct. Sequence provided here as reference only.
mutable notes: Bacillus shuttle vector. Includes pSPANK and b0010 terminator, both of which are integrated into our final construct.
mutable provenance: Designed and assembled by a member of our supporting lab, PJ steiner.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K911010_sequence/1

pSB1C3-PP
type: ComponentDefinition
persistent identity: BBa K678069
display id: BBa_K678069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3-PP
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Linker 1
sequence annotation: Linker 6
sequence annotation: backbone
sequence: https://synbiohub.org/public/igem/BBa_K678069_sequence/1

Linker 1
type: SequenceAnnotation
persistent identity: annotation2127649
display id: annotation2127649
version: 1
top level: pSB1C3-PP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678069/annotation2127649/range2127649/1

https://synbiohub.org/public/igem/BBa_K678069/annotation2127649/range2127649/1
type: Range
persistent identity: range2127649
display id: range2127649
version: 1
direction: direction/forward
top level: pSB1C3-PP
start: 2079
end: 2086
orientation: inline

Linker 6
type: SequenceAnnotation
persistent identity: annotation2127647
display id: annotation2127647
version: 1
top level: pSB1C3-PP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678069/annotation2127647/range2127647/1

https://synbiohub.org/public/igem/BBa_K678069/annotation2127647/range2127647/1
type: Range
persistent identity: range2127647
display id: range2127647
version: 1
direction: direction/forward
top level: pSB1C3-PP
start: 1
end: 8
orientation: inline

backbone
type: SequenceAnnotation
persistent identity: annotation2127648
display id: annotation2127648
version: 1
top level: pSB1C3-PP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678069/annotation2127648/range2127648/1

https://synbiohub.org/public/igem/BBa_K678069/annotation2127648/range2127648/1
type: Range
persistent identity: range2127648
display id: range2127648
version: 1
direction: direction/forward
top level: pSB1C3-PP
start: 9
end: 2078
orientation: inline

BBa_K1648004
type: ComponentDefinition
persistent identity: BBa K1648004
display id: BBa_K1648004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane
creator: Leung King Pong
created: 2015-07-16T11:00:00Z
modified: 2015-09-23T11:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2065_
m user id: 26823
owner id: 26823
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Consist of six fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome. Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamXY, mamGFDC and mms6 operons''' except the mamC operon is designed for '''homologous recombination'''. Part containing this template ([http://parts.igem.org/Part:BBa_K1648002 K1648002]) together with another part containing mamC ([http://parts.igem.org/Part:BBa_K1648XXX K1648XXX]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''.
mutable notes: In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from '''mamGFDC''', '''mamYXZ-ftsZ''' and '''mms6-mmsF-mms35-mms48''' ('''mamGC''', '''mamXY''' and '''mms6 operons''') except the mamC operon as flanking sequences. Fragments are joined via overlapping PCR.
mutable provenance: Magnetospirillum gryphiswaldense MSR-1 v2, complete genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: polya
sequence annotation: BBa_B0010
sequence annotation: stop
sequence annotation: BBa_J13002
sequence annotation: stem_loop
sequence annotation: BBa_B0015
sequence annotation: mamC
sequence annotation: BBa_B0012
sequence annotation: -10
sequence annotation: TetR 1
sequence annotation: BBa_K1648007
sequence annotation: conserved
sequence annotation: BBa_B0034
sequence annotation: T7 TE
sequence annotation: TetR 2
sequence annotation: BBa_R0040
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K1648004_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation2463399
display id: annotation2463399
version: 1
top level: BBa_K1648004
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463399/range2463399/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463399/range2463399/1
type: Range
persistent identity: range2463399
display id: range2463399
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 579
end: 592
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2463395
display id: annotation2463395
version: 1
top level: BBa_K1648004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463395/range2463395/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463395/range2463395/1
type: Range
persistent identity: range2463395
display id: range2463395
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 464
end: 543
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2463400
display id: annotation2463400
version: 1
top level: BBa_K1648004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463400/range2463400/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463400/range2463400/1
type: Range
persistent identity: range2463400
display id: range2463400
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 585
end: 585
orientation: inline

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2463392
display id: annotation2463392
version: 1
top level: BBa_K1648004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463392/range2463392/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463392/range2463392/1
type: Range
persistent identity: range2463392
display id: range2463392
version: 1
direction: direction/reverse
top level: BBa_K1648004
start: 1
end: 74
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2463396
display id: annotation2463396
version: 1
top level: BBa_K1648004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463396/range2463396/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463396/range2463396/1
type: Range
persistent identity: range2463396
display id: range2463396
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 475
end: 518
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2463401
display id: annotation2463401
version: 1
top level: BBa_K1648004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463401/range2463401/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463401/range2463401/1
type: Range
persistent identity: range2463401
display id: range2463401
version: 1
direction: direction/reverse
top level: BBa_K1648004
start: 464
end: 592
orientation: inline

mamC
type: SequenceAnnotation
persistent identity: annotation2463393
display id: annotation2463393
version: 1
top level: BBa_K1648004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463393/range2463393/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463393/range2463393/1
type: Range
persistent identity: range2463393
display id: range2463393
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 81
end: 455
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2463397
display id: annotation2463397
version: 1
top level: BBa_K1648004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463397/range2463397/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463397/range2463397/1
type: Range
persistent identity: range2463397
display id: range2463397
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 552
end: 592
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2463389
display id: annotation2463389
version: 1
top level: BBa_K1648004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463389/range2463389/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463389/range2463389/1
type: Range
persistent identity: range2463389
display id: range2463389
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 43
end: 48
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2463385
display id: annotation2463385
version: 1
top level: BBa_K1648004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463385/range2463385/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463385/range2463385/1
type: Range
persistent identity: range2463385
display id: range2463385
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 1
end: 19
orientation: inline

BBa_K1648007
type: SequenceAnnotation
persistent identity: annotation2463394
display id: annotation2463394
version: 1
top level: BBa_K1648004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463394/range2463394/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463394/range2463394/1
type: Range
persistent identity: range2463394
display id: range2463394
version: 1
direction: direction/reverse
top level: BBa_K1648004
start: 81
end: 455
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2463391
display id: annotation2463391
version: 1
top level: BBa_K1648004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463391/range2463391/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463391/range2463391/1
type: Range
persistent identity: range2463391
display id: range2463391
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 67
end: 70
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463390
display id: annotation2463390
version: 1
top level: BBa_K1648004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463390/range2463390/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463390/range2463390/1
type: Range
persistent identity: range2463390
display id: range2463390
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 63
end: 74
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2463398
display id: annotation2463398
version: 1
top level: BBa_K1648004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463398/range2463398/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463398/range2463398/1
type: Range
persistent identity: range2463398
display id: range2463398
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 559
end: 578
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2463388
display id: annotation2463388
version: 1
top level: BBa_K1648004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463388/range2463388/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463388/range2463388/1
type: Range
persistent identity: range2463388
display id: range2463388
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 26
end: 44
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2463386
display id: annotation2463386
version: 1
top level: BBa_K1648004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463386/range2463386/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463386/range2463386/1
type: Range
persistent identity: range2463386
display id: range2463386
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 1
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2463387
display id: annotation2463387
version: 1
top level: BBa_K1648004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1648004/annotation2463387/range2463387/1

https://synbiohub.org/public/igem/BBa_K1648004/annotation2463387/range2463387/1
type: Range
persistent identity: range2463387
display id: range2463387
version: 1
direction: direction/forward
top level: BBa_K1648004
start: 20
end: 25
orientation: inline

BBa_K1137005
type: ComponentDefinition
persistent identity: BBa K1137005
display id: BBa_K1137005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB10K1
creator: Matthew Deyell
created: 2013-10-03T11:00:00Z
modified: 2015-05-08T01:09:21Z
discontinued: false
dominant: false
group u list: _1449_
m user id: 0
owner id: 15098
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB10K1 ??? contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with ColA replicon with a copy number of 20 ??? 40 per cell. The cat gene has been replaced with kan cassette conveying kanamycin resistance.
mutable notes: Designed to be functional with pSB9S1, pSB3C7, and pSB6A3
mutable provenance: pSB1C3 is the template with the ori and resistance replaced with those from pCOLA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Terminator
sequence annotation: ColA
sequence annotation: Suffix
sequence annotation: Prefix
sequence annotation: mRFP
sequence annotation: Kan
sequence: https://synbiohub.org/public/igem/BBa_K1137005_sequence/1

Terminator
type: SequenceAnnotation
persistent identity: annotation2367907
display id: annotation2367907
version: 1
top level: BBa_K1137005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137005/annotation2367907/range2367907/1

https://synbiohub.org/public/igem/BBa_K1137005/annotation2367907/range2367907/1
type: Range
persistent identity: range2367907
display id: range2367907
version: 1
direction: direction/forward
top level: BBa_K1137005
start: 1137
end: 1205
orientation: inline

ColA
type: SequenceAnnotation
persistent identity: annotation2367906
display id: annotation2367906
version: 1
top level: BBa_K1137005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1137005/annotation2367906/range2367906/1

https://synbiohub.org/public/igem/BBa_K1137005/annotation2367906/range2367906/1
type: Range
persistent identity: range2367906
display id: range2367906
version: 1
direction: direction/reverse
top level: BBa_K1137005
start: 1278
end: 1913
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2367897
display id: annotation2367897
version: 1
top level: BBa_K1137005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1137005/annotation2367897/range2367897/1

https://synbiohub.org/public/igem/BBa_K1137005/annotation2367897/range2367897/1
type: Range
persistent identity: range2367897
display id: range2367897
version: 1
direction: direction/forward
top level: BBa_K1137005
start: 1092
end: 1111
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2367908
display id: annotation2367908
version: 1
top level: BBa_K1137005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137005/annotation2367908/range2367908/1

https://synbiohub.org/public/igem/BBa_K1137005/annotation2367908/range2367908/1
type: Range
persistent identity: range2367908
display id: range2367908
version: 1
direction: direction/forward
top level: BBa_K1137005
start: 1226
end: 1266
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2367910
display id: annotation2367910
version: 1
top level: BBa_K1137005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137005/annotation2367910/range2367910/1

https://synbiohub.org/public/igem/BBa_K1137005/annotation2367910/range2367910/1
type: Range
persistent identity: range2367910
display id: range2367910
version: 1
direction: direction/forward
top level: BBa_K1137005
start: 3078
end: 3149
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2367895
display id: annotation2367895
version: 1
top level: BBa_K1137005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1137005/annotation2367895/range2367895/1

https://synbiohub.org/public/igem/BBa_K1137005/annotation2367895/range2367895/1
type: Range
persistent identity: range2367895
display id: range2367895
version: 1
direction: direction/forward
top level: BBa_K1137005
start: 1
end: 20
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2367896
display id: annotation2367896
version: 1
top level: BBa_K1137005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1137005/annotation2367896/range2367896/1

https://synbiohub.org/public/igem/BBa_K1137005/annotation2367896/range2367896/1
type: Range
persistent identity: range2367896
display id: range2367896
version: 1
direction: direction/forward
top level: BBa_K1137005
start: 248
end: 925
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2367911
display id: annotation2367911
version: 1
top level: BBa_K1137005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137005/annotation2367911/range2367911/1

https://synbiohub.org/public/igem/BBa_K1137005/annotation2367911/range2367911/1
type: Range
persistent identity: range2367911
display id: range2367911
version: 1
direction: direction/forward
top level: BBa_K1137005
start: 3170
end: 3247
orientation: inline

Kan
type: SequenceAnnotation
persistent identity: annotation2367909
display id: annotation2367909
version: 1
top level: BBa_K1137005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1137005/annotation2367909/range2367909/1

https://synbiohub.org/public/igem/BBa_K1137005/annotation2367909/range2367909/1
type: Range
persistent identity: range2367909
display id: range2367909
version: 1
direction: direction/forward
top level: BBa_K1137005
start: 2023
end: 2838
orientation: inline

BBa_J23019
type: ComponentDefinition
persistent identity: BBa J23019
display id: BBa_J23019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"
creator: John Anderson
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Variant of pBca1021 (J61003) with GFP inserted. Part J23019 is the same as J23018 but in pSB3C6.
mutable notes: N/A
mutable provenance: J23018 pAC997
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rrnB T1 terminator
sequence annotation: BBa_B0034 RBS
sequence annotation: BioBrick suffix
sequence annotation: RBS
sequence annotation: BBa_R0040 (Ptet)
sequence annotation: EcoRI
sequence annotation: chloramphenicol acetyl transferase (CmR resistance marker)
sequence annotation: NotI
sequence annotation: p15A origin
sequence annotation: XbaI
sequence annotation: BBa_B0015 terminator
sequence annotation: Ptet
sequence: https://synbiohub.org/public/igem/BBa_J23019_sequence/1

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975743
display id: annotation1975743
version: 1
top level: BBa_J23019
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975743/range1975743/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975743/range1975743/1
type: Range
persistent identity: range1975743
display id: range1975743
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2077
end: 2120
orientation: inline

BBa_B0034 RBS
type: SequenceAnnotation
persistent identity: annotation1975759
display id: annotation1975759
version: 1
top level: BBa_J23019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975759/range1975759/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975759/range1975759/1
type: Range
persistent identity: range1975759
display id: range1975759
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2265
end: 2276
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1893440
display id: annotation1893440
version: 1
top level: BBa_J23019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1893440/range1893440/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1893440/range1893440/1
type: Range
persistent identity: range1893440
display id: range1893440
version: 1
direction: direction/forward
top level: BBa_J23019
start: 1
end: 21
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975758
display id: annotation1975758
version: 1
top level: BBa_J23019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975758/range1975758/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975758/range1975758/1
type: Range
persistent identity: range1975758
display id: range1975758
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2265
end: 2276
orientation: inline

BBa_R0040 (Ptet)
type: SequenceAnnotation
persistent identity: annotation1893439
display id: annotation1893439
version: 1
top level: BBa_J23019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1893439/range1893439/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1893439/range1893439/1
type: Range
persistent identity: range1893439
display id: range1893439
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2203
end: 2256
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975744
display id: annotation1975744
version: 1
top level: BBa_J23019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975744/range1975744/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975744/range1975744/1
type: Range
persistent identity: range1975744
display id: range1975744
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2044
end: 2049
orientation: inline

chloramphenicol acetyl transferase (CmR resistance marker)
type: SequenceAnnotation
persistent identity: annotation1975742
display id: annotation1975742
version: 1
top level: BBa_J23019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975742/range1975742/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975742/range1975742/1
type: Range
persistent identity: range1975742
display id: range1975742
version: 1
direction: direction/forward
top level: BBa_J23019
start: 1232
end: 1891
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975745
display id: annotation1975745
version: 1
top level: BBa_J23019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975745/range1975745/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975745/range1975745/1
type: Range
persistent identity: range1975745
display id: range1975745
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2050
end: 2057
orientation: inline

p15A origin
type: SequenceAnnotation
persistent identity: annotation1975741
display id: annotation1975741
version: 1
top level: BBa_J23019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975741/range1975741/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975741/range1975741/1
type: Range
persistent identity: range1975741
display id: range1975741
version: 1
direction: direction/reverse
top level: BBa_J23019
start: 22
end: 870
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975746
display id: annotation1975746
version: 1
top level: BBa_J23019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975746/range1975746/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975746/range1975746/1
type: Range
persistent identity: range1975746
display id: range1975746
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2278
end: 2283
orientation: inline

BBa_B0015 terminator
type: SequenceAnnotation
persistent identity: annotation1893438
display id: annotation1893438
version: 1
top level: BBa_J23019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1893438/range1893438/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1893438/range1893438/1
type: Range
persistent identity: range1893438
display id: range1893438
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2066
end: 2194
orientation: inline

Ptet
type: SequenceAnnotation
persistent identity: annotation1975762
display id: annotation1975762
version: 1
top level: BBa_J23019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975762/range1975762/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975762/range1975762/1
type: Range
persistent identity: range1975762
display id: range1975762
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2203
end: 2256
orientation: inline

Cyc1P Acce
type: ComponentDefinition
persistent identity: BBa K106014
display id: BBa_K106014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, Cyc1P, Adh1t)
creator: Sergio Peisajovich
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Acceptor vector for AarI based cloning. Accepts any combination of parts beginning with an A site and ending with a D site. Expression of construct is driven by a Cyc1 promoter, with Adh1 terminator.
mutable notes: The pRS315 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites. Please note, the actual orientation of this vector is reversed.
mutable provenance: A modified pRS315 plasmid--CEN ARS yeast expression vector (LEU2 marker).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: A overhang
sequence annotation: f1 origin
sequence annotation: lacZalpha reporter
sequence annotation: Cyc1 Promoter
sequence annotation: T3 promoter
sequence annotation: LEU2 marker
sequence annotation: Stop codons in all 3 frames
sequence annotation: ampicillin resistance marker
sequence annotation: ampicillin resistance promoter
sequence annotation: T7 promoter
sequence annotation: D overhang
sequence annotation: lac promoter
sequence annotation: Start codon
sequence annotation: pBR322 origin
sequence annotation: Adh1 terminator
sequence annotation: CEN ARS
sequence: https://synbiohub.org/public/igem/BBa_K106014_sequence/1

A overhang
type: SequenceAnnotation
persistent identity: annotation1999685
display id: annotation1999685
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999685/range1999685/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999685/range1999685/1
type: Range
persistent identity: range1999685
display id: range1999685
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6394
end: 6397
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999688
display id: annotation1999688
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999688/range1999688/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999688/range1999688/1
type: Range
persistent identity: range1999688
display id: range1999688
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 427
end: 733
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999687
display id: annotation1999687
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999687/range1999687/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999687/range1999687/1
type: Range
persistent identity: range1999687
display id: range1999687
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 246
end: 405
orientation: inline

Cyc1 Promoter
type: SequenceAnnotation
persistent identity: annotation1986801
display id: annotation1986801
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986801/range1986801/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986801/range1986801/1
type: Range
persistent identity: range1986801
display id: range1986801
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6132
end: 6394
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999692
display id: annotation1999692
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999692/range1999692/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999692/range1999692/1
type: Range
persistent identity: range1999692
display id: range1999692
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6095
end: 6114
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1986804
display id: annotation1986804
version: 1
top level: Cyc1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986804/range1986804/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986804/range1986804/1
type: Range
persistent identity: range1986804
display id: range1986804
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 1570
end: 2676
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999703
display id: annotation1999703
version: 1
top level: Cyc1P Acce
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999703/range1999703/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999703/range1999703/1
type: Range
persistent identity: range1999703
display id: range1999703
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 11
end: 21
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1986800
display id: annotation1986800
version: 1
top level: Cyc1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986800/range1986800/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986800/range1986800/1
type: Range
persistent identity: range1986800
display id: range1986800
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 4053
end: 4911
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999689
display id: annotation1999689
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999689/range1999689/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999689/range1999689/1
type: Range
persistent identity: range1999689
display id: range1999689
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 3983
end: 4011
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999686
display id: annotation1999686
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999686/range1999686/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999686/range1999686/1
type: Range
persistent identity: range1999686
display id: range1999686
version: 1
direction: direction/reverse
top level: Cyc1P Acce
start: 224
end: 242
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1986802
display id: annotation1986802
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986802/range1986802/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986802/range1986802/1
type: Range
persistent identity: range1986802
display id: range1986802
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 1
end: 4
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999691
display id: annotation1999691
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999691/range1999691/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999691/range1999691/1
type: Range
persistent identity: range1999691
display id: range1999691
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 5996
end: 6025
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999704
display id: annotation1999704
version: 1
top level: Cyc1P Acce
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999704/range1999704/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999704/range1999704/1
type: Range
persistent identity: range1999704
display id: range1999704
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6385
end: 6387
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999690
display id: annotation1999690
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999690/range1999690/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999690/range1999690/1
type: Range
persistent identity: range1999690
display id: range1999690
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 5068
end: 5687
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1986803
display id: annotation1986803
version: 1
top level: Cyc1P Acce
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986803/range1986803/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986803/range1986803/1
type: Range
persistent identity: range1986803
display id: range1986803
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 23
end: 216
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1986799
display id: annotation1986799
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986799/range1986799/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986799/range1986799/1
type: Range
persistent identity: range1986799
display id: range1986799
version: 1
direction: direction/reverse
top level: Cyc1P Acce
start: 3403
end: 3921
orientation: inline

BBa_K289009
type: ComponentDefinition
persistent identity: BBa K289009
display id: BBa_K289009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGADT7 is the AD Vector included with MATCHMAKER Two-Hybrid System 3.
creator: Cai Hong
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _393_
m user id: 0
owner id: 5128
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The pGADT7 vector expresses proteins fused to amino acids 768???881 of the GAL4 activation domain (AD). In yeast, fusion proteins are expressed at high levels from the constitutive ADH1 promoter (PADH1); transcription is terminated at the ADH1 transcription termination signal (TADH1). The fusion protein is targeted to the yeast nucleus by the SV40 nuclear localization sequences that have been added to the activation domain sequence (1). pGADT7 also contains the T7 promoter, an HA epitope tag, and a MCS. pGADT7 replicates autonomously in both E. coli and S. cerevisiae from the pUC and 2 m ori, respectively. The vector carries Ampr for selection in E. coli and the LEU2 nutritional marker for selection in yeast.
mutable notes: Propagation in E. coli: ??? Suitable host strains: DH5a, DH10 & other general purpose strains ??? Selectable marker: plasmid confers resistance to ampicillin (100 mg/ml) to E. coli hosts ??? E. coli replication origin: pUC ??? Copy number: ~500 ??? Plasmid incompatibility group: pMB1/Col E1 Propagation in S. cerevisiae: ??? Suitable host strains: Y187(a), Y190(a), SFY526(a), CG1945(a), HF7c(a), or AH109(a) ??? Selectable marker: LEU2 ??? S. cerevisiae origin: 2 m
mutable provenance: Commercial
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K289009_sequence/1

BBa_K864014
type: ComponentDefinition
persistent identity: BBa K864014
display id: BBa_K864014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864014_sequence/1

BBa_J72209
type: ComponentDefinition
persistent identity: BBa J72209
display id: BBa_J72209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kanamycin + ampicillin resistant p15A vector for 2ab assembly
creator: Josh Kittleson
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:08:29Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: kanamycin + ampicillin resistant p15A vector, used for 2ab assembly referred to internally as pBjh1601KA.
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72209_sequence/1

BBa_J72114
type: ComponentDefinition
persistent identity: BBa J72114
display id: BBa_J72114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Complete, arabinose inducible phagemid, CmR, p15a vector
creator: Josh Kittleson
created: 2011-11-09T12:00:00Z
modified: 2015-05-08T01:08:27Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Vector contains all of the cis elements required to be an efficient phagemid. Arabinose induces lysis.
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72114_sequence/1

pSB1A8
type: ComponentDefinition
persistent identity: BBa J119043
display id: BBa_J119043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A8
creator: Todd Eckdahl and A. Malcolm Campbell
created: 2012-01-04T12:00:00Z
modified: 2015-08-31T04:08:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _613_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Insulated vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix.
mutable notes: BsaI site removed from amplicillin resistance gene.
mutable provenance: De novo DNA synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: suffix
sequence annotation: B1010 terminator
sequence annotation: VF2 primer
sequence annotation: B0025 terminator
sequence annotation: mutation A --> G removes BsaI site
sequence annotation: stuffer
sequence annotation: prefix
sequence annotation: Amp Resistance (rev)
sequence annotation: pMB1 replication origin (rev)
sequence annotation: VR primer
sequence: https://synbiohub.org/public/igem/BBa_J119043_sequence/1

suffix
type: SequenceAnnotation
persistent identity: annotation2167640
display id: annotation2167640
version: 1
top level: pSB1A8
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119043/annotation2167640/range2167640/1

https://synbiohub.org/public/igem/BBa_J119043/annotation2167640/range2167640/1
type: Range
persistent identity: range2167640
display id: range2167640
version: 1
direction: direction/forward
top level: pSB1A8
start: 1
end: 21
orientation: inline

B1010 terminator
type: SequenceAnnotation
persistent identity: annotation2167643
display id: annotation2167643
version: 1
top level: pSB1A8
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119043/annotation2167643/range2167643/1

https://synbiohub.org/public/igem/BBa_J119043/annotation2167643/range2167643/1
type: Range
persistent identity: range2167643
display id: range2167643
version: 1
direction: direction/forward
top level: pSB1A8
start: 2167
end: 2206
orientation: inline

VF2 primer
type: SequenceAnnotation
persistent identity: annotation2167685
display id: annotation2167685
version: 1
top level: pSB1A8
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119043/annotation2167685/range2167685/1

https://synbiohub.org/public/igem/BBa_J119043/annotation2167685/range2167685/1
type: Range
persistent identity: range2167685
display id: range2167685
version: 1
direction: direction/forward
top level: pSB1A8
start: 2069
end: 2088
orientation: inline

B0025 terminator
type: SequenceAnnotation
persistent identity: annotation2167642
display id: annotation2167642
version: 1
top level: pSB1A8
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119043/annotation2167642/range2167642/1

https://synbiohub.org/public/igem/BBa_J119043/annotation2167642/range2167642/1
type: Range
persistent identity: range2167642
display id: range2167642
version: 1
direction: direction/forward
top level: pSB1A8
start: 22
end: 205
orientation: inline

mutation A --> G removes BsaI site
type: SequenceAnnotation
persistent identity: annotation2167753
display id: annotation2167753
version: 1
top level: pSB1A8
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J119043/annotation2167753/range2167753/1

https://synbiohub.org/public/igem/BBa_J119043/annotation2167753/range2167753/1
type: Range
persistent identity: range2167753
display id: range2167753
version: 1
direction: direction/forward
top level: pSB1A8
start: 1227
end: 1227
orientation: inline

stuffer
type: SequenceAnnotation
persistent identity: annotation2167688
display id: annotation2167688
version: 1
top level: pSB1A8
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119043/annotation2167688/range2167688/1

https://synbiohub.org/public/igem/BBa_J119043/annotation2167688/range2167688/1
type: Range
persistent identity: range2167688
display id: range2167688
version: 1
direction: direction/forward
top level: pSB1A8
start: 2228
end: 2240
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2167641
display id: annotation2167641
version: 1
top level: pSB1A8
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119043/annotation2167641/range2167641/1

https://synbiohub.org/public/igem/BBa_J119043/annotation2167641/range2167641/1
type: Range
persistent identity: range2167641
display id: range2167641
version: 1
direction: direction/forward
top level: pSB1A8
start: 2207
end: 2228
orientation: inline

Amp Resistance (rev)
type: SequenceAnnotation
persistent identity: annotation2167687
display id: annotation2167687
version: 1
top level: pSB1A8
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J119043/annotation2167687/range2167687/1

https://synbiohub.org/public/igem/BBa_J119043/annotation2167687/range2167687/1
type: Range
persistent identity: range2167687
display id: range2167687
version: 1
direction: direction/reverse
top level: pSB1A8
start: 1085
end: 1946
orientation: inline

pMB1 replication origin (rev)
type: SequenceAnnotation
persistent identity: annotation2167686
display id: annotation2167686
version: 1
top level: pSB1A8
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J119043/annotation2167686/range2167686/1

https://synbiohub.org/public/igem/BBa_J119043/annotation2167686/range2167686/1
type: Range
persistent identity: range2167686
display id: range2167686
version: 1
direction: direction/forward
top level: pSB1A8
start: 310
end: 923
orientation: inline

VR primer
type: SequenceAnnotation
persistent identity: annotation2167684
display id: annotation2167684
version: 1
top level: pSB1A8
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119043/annotation2167684/range2167684/1

https://synbiohub.org/public/igem/BBa_J119043/annotation2167684/range2167684/1
type: Range
persistent identity: range2167684
display id: range2167684
version: 1
direction: direction/reverse
top level: pSB1A8
start: 208
end: 227
orientation: inline

BBa_K812001
type: ComponentDefinition
persistent identity: BBa K812001
display id: BBa_K812001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biobricked pSC2+ plasmid with pElastase promoter for frog use
creator: Cyrille Pauthenier
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:27Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1069_
m user id: 0
owner id: 8998
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Biobricked pSC2+ plasmid with a pElastase promoter for frogs This plasmid is a biobricked version of the standard plasmid pSC2+ with the pElastase promoter. This is a daugter par of K812001. This plasmid is made of a bacterial ampicilin reststant backbone, with a universal CMV promoter. The Biobrick site is surrounded by a a 5'UTR expression enhancer, and a ploy-A tail with a SV40 3' UTR that tnhance the stabilisation of the mRNA and the expression of the protein. This plasmid is ment to be micro-injected in frog eggs or chicken eggs for a transcient expression of the protein at the early ages of development of the embryo. This plasmid does not replicate, so it gets diluted and degraded as the animal grow, leaving no chance for DNA propagation to the breeding. To use this plasmid, you simply have to insert your protein between the EcoRI and PstI site. It is better to add a kozac sequence in front of your gene to enhance its expression. This plasmid contains the standard registry insert J04450 as a negative cloning control.
mutable notes: This is the daughter part of K812000 where the CMV promoter has been replaced by the pElastase promoter cutting using HindIII and SalI unique site
mutable provenance: This plasmid is the biobricked version of pSC2+ where the promoter has been replaced. Check the original part K812001 for more information.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

BBa_K287007
type: ComponentDefinition
persistent identity: BBa K287007
display id: BBa_K287007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid of pBSK II
creator: Carlos Andres Sanchez
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
group u list: _390_
m user id: 0
owner id: 4706
owning group id: 9
sample status: No part sequence
status: status/Unavailable
bookmark: false
mutable description: This is a plasmid of pBSK II. Resistant to ampiciline
mutable notes: None
mutable provenance: This part comes from a Genomic Sequence from the NCBI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

BBa_K268001
type: ComponentDefinition
persistent identity: BBa K268001
display id: BBa_K268001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS316
creator: Nora Yucel
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _379_
m user id: 0
owner id: 2866
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Empty plasmid for gene expression in S. cerevisiae. Ura selection in yeast and ampicillan resistance in E. coli. One of a series of pBluescript-based centromere vectors (ATCC 77142 ATCC 77145, ATCC 77157 to ATCC 77158) differing in the yeast selectable marker gene. The order of the major features in this plasmid is: TRP1 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla - CEN6 - ARSH4. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; BamHI--4.8; PvuII--4.2, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416309 Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH499, Saccharomyces cerevisiae YPH500, Saccharomyces cerevisiae YPH501. Related vectors: pRS313, pRS315, pRS316.
mutable notes: Eliminated PstI, XbaI and SpeI restriction sites.
mutable provenance: From ATCC. Pubmed accession number U03442.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K268001_sequence/1

pSB6A1
type: ComponentDefinition
persistent identity: pSB6A1
display id: pSB6A1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBR322 converted to BioBrick vector (formally BBa_I739201)
creator: Stefan Luzi
created: 2007-10-22T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: sadf
mutable notes: asdf
mutable provenance: sdf
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RNAI transcript promoter -10 sequence
sequence annotation: rep pMB1
sequence annotation: rop
sequence annotation: L-strand Y effector site
sequence annotation: BioBrick prefix
sequence annotation: partial tetracycline resistance CDS (nonfunctional)
sequence annotation: beta-lactamase signal peptide
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: RNAII transcript
sequence annotation: from pSC101
sequence annotation: RNAI transcript
sequence annotation: bla(P3) promoter -35 sequence
sequence annotation: bla(P3) promoter -10 sequence
sequence annotation: bla gene
sequence annotation: gamma-delta insertion sequence
sequence annotation: beta-lactamase mature peptide
sequence annotation: H-strand Y effector site
sequence annotation: beta-lactamase (bla gene)
sequence annotation: BioBrick suffix
sequence annotation: Repeat region 1
sequence annotation: RNAI transcript promoter -35 sequence
sequence annotation: RNAII transcript promoter -35 sequence
sequence: https://synbiohub.org/public/igem/pSB6A1_sequence/1

RNAI transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969649
display id: annotation1969649
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969649/range1969649/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969649/range1969649/1
type: Range
persistent identity: range1969649
display id: range1969649
version: 1
direction: direction/forward
top level: pSB6A1
start: 2604
end: 2609
orientation: inline

rep pMB1
type: SequenceAnnotation
persistent identity: annotation1969645
display id: annotation1969645
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969645/range1969645/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969645/range1969645/1
type: Range
persistent identity: range1969645
display id: range1969645
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2161
end: 2775
orientation: inline

rop
type: SequenceAnnotation
persistent identity: annotation1969641
display id: annotation1969641
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969641/range1969641/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969641/range1969641/1
type: Range
persistent identity: range1969641
display id: range1969641
version: 1
direction: direction/forward
top level: pSB6A1
start: 1557
end: 1748
orientation: inline

L-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1969643
display id: annotation1969643
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969643/range1969643/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969643/range1969643/1
type: Range
persistent identity: range1969643
display id: range1969643
version: 1
direction: direction/reverse
top level: pSB6A1
start: 1993
end: 2056
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969658
display id: annotation1969658
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969658/range1969658/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969658/range1969658/1
type: Range
persistent identity: range1969658
display id: range1969658
version: 1
direction: direction/forward
top level: pSB6A1
start: 4001
end: 4022
orientation: inline

partial tetracycline resistance CDS (nonfunctional)
type: SequenceAnnotation
persistent identity: annotation1969638
display id: annotation1969638
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969638/range1969638/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969638/range1969638/1
type: Range
persistent identity: range1969638
display id: range1969638
version: 1
direction: direction/forward
top level: pSB6A1
start: 23
end: 918
orientation: inline

beta-lactamase signal peptide
type: SequenceAnnotation
persistent identity: annotation1969652
display id: annotation1969652
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969652/range1969652/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969652/range1969652/1
type: Range
persistent identity: range1969652
display id: range1969652
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3727
end: 3795
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969650
display id: annotation1969650
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969650/range1969650/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969650/range1969650/1
type: Range
persistent identity: range1969650
display id: range1969650
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2738
end: 2743
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1969644
display id: annotation1969644
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969644/range1969644/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969644/range1969644/1
type: Range
persistent identity: range1969644
display id: range1969644
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2176
end: 2728
orientation: inline

from pSC101
type: SequenceAnnotation
persistent identity: annotation1969640
display id: annotation1969640
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969640/range1969640/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969640/range1969640/1
type: Range
persistent identity: range1969640
display id: range1969640
version: 1
direction: direction/forward
top level: pSB6A1
start: 1278
end: 1404
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1969646
display id: annotation1969646
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969646/range1969646/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969646/range1969646/1
type: Range
persistent identity: range1969646
display id: range1969646
version: 1
direction: direction/forward
top level: pSB6A1
start: 2618
end: 2725
orientation: inline

bla(P3) promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969656
display id: annotation1969656
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969656/range1969656/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969656/range1969656/1
type: Range
persistent identity: range1969656
display id: range1969656
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3860
end: 3865
orientation: inline

bla(P3) promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969655
display id: annotation1969655
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969655/range1969655/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969655/range1969655/1
type: Range
persistent identity: range1969655
display id: range1969655
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3839
end: 3844
orientation: inline

bla gene
type: SequenceAnnotation
persistent identity: annotation1969654
display id: annotation1969654
version: 1
top level: pSB6A1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969654/range1969654/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969654/range1969654/1
type: Range
persistent identity: range1969654
display id: range1969654
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3803
end: 3807
orientation: inline

gamma-delta insertion sequence
type: SequenceAnnotation
persistent identity: annotation1969639
display id: annotation1969639
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969639/range1969639/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969639/range1969639/1
type: Range
persistent identity: range1969639
display id: range1969639
version: 1
direction: direction/reverse
top level: pSB6A1
start: 1157
end: 1161
orientation: inline

beta-lactamase mature peptide
type: SequenceAnnotation
persistent identity: annotation1969653
display id: annotation1969653
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969653/range1969653/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969653/range1969653/1
type: Range
persistent identity: range1969653
display id: range1969653
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2938
end: 3726
orientation: inline

H-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1969642
display id: annotation1969642
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969642/range1969642/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969642/range1969642/1
type: Range
persistent identity: range1969642
display id: range1969642
version: 1
direction: direction/forward
top level: pSB6A1
start: 1653
end: 1809
orientation: inline

beta-lactamase (bla gene)
type: SequenceAnnotation
persistent identity: annotation1969647
display id: annotation1969647
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969647/range1969647/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969647/range1969647/1
type: Range
persistent identity: range1969647
display id: range1969647
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2935
end: 3795
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969637
display id: annotation1969637
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969637/range1969637/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969637/range1969637/1
type: Range
persistent identity: range1969637
display id: range1969637
version: 1
direction: direction/forward
top level: pSB6A1
start: 1
end: 21
orientation: inline

Repeat region 1
type: SequenceAnnotation
persistent identity: annotation1969657
display id: annotation1969657
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969657/range1969657/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969657/range1969657/1
type: Range
persistent identity: range1969657
display id: range1969657
version: 1
direction: direction/forward
top level: pSB6A1
start: 2790
end: 4003
orientation: inline

RNAI transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969648
display id: annotation1969648
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969648/range1969648/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969648/range1969648/1
type: Range
persistent identity: range1969648
display id: range1969648
version: 1
direction: direction/forward
top level: pSB6A1
start: 2582
end: 2587
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969651
display id: annotation1969651
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969651/range1969651/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969651/range1969651/1
type: Range
persistent identity: range1969651
display id: range1969651
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2759
end: 2764
orientation: inline

BBa_J72110
type: ComponentDefinition
persistent identity: BBa J72110
display id: BBa_J72110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AmpR/CamR p15A vector
creator: Josh Kittleson
created: 2011-11-09T12:00:00Z
modified: 2015-05-08T01:08:27Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: na
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72110_sequence/1

BBa_K797013
type: ComponentDefinition
persistent identity: BBa K797013
display id: BBa_K797013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid Backbone for golden gate assembly
creator: Tomohiro Nobeyama
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1053_
m user id: 0
owner id: 11588
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This backbone is suitable for golden gate assembly. Golden gate assembly is a suitable assembly to combine many parts in order you want. This plasmid backbone have restriction enzyme cutting site of Dpn1 let you build construction by Golden gate assembly in one ligation.
mutable notes: The terminal of this part was modified to use it for Golden gate assembly
mutable provenance: This part was made by PCR from iGEM part BBa_PSB1K3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BsaI Recognition site
sequence annotation: BioBrick suffix
sequence annotation: BsaI Restriction site
sequence annotation: BioBrick prefix
sequence annotation: VF2
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_K797013_sequence/1

BsaI Recognition site
type: SequenceAnnotation
persistent identity: annotation2196005
display id: annotation2196005
version: 1
top level: BBa_K797013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K797013/annotation2196005/range2196005/1

https://synbiohub.org/public/igem/BBa_K797013/annotation2196005/range2196005/1
type: Range
persistent identity: range2196005
display id: range2196005
version: 1
direction: direction/reverse
top level: BBa_K797013
start: 2224
end: 2229
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2196000
display id: annotation2196000
version: 1
top level: BBa_K797013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K797013/annotation2196000/range2196000/1

https://synbiohub.org/public/igem/BBa_K797013/annotation2196000/range2196000/1
type: Range
persistent identity: range2196000
display id: range2196000
version: 1
direction: direction/forward
top level: BBa_K797013
start: 15
end: 35
orientation: inline

BsaI Recognition site
type: SequenceAnnotation
persistent identity: annotation2195998
display id: annotation2195998
version: 1
top level: BBa_K797013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K797013/annotation2195998/range2195998/1

https://synbiohub.org/public/igem/BBa_K797013/annotation2195998/range2195998/1
type: Range
persistent identity: range2195998
display id: range2195998
version: 1
direction: direction/forward
top level: BBa_K797013
start: 4
end: 9
orientation: inline

BsaI Restriction site
type: SequenceAnnotation
persistent identity: annotation2195999
display id: annotation2195999
version: 1
top level: BBa_K797013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K797013/annotation2195999/range2195999/1

https://synbiohub.org/public/igem/BBa_K797013/annotation2195999/range2195999/1
type: Range
persistent identity: range2195999
display id: range2195999
version: 1
direction: direction/forward
top level: BBa_K797013
start: 11
end: 14
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2196003
display id: annotation2196003
version: 1
top level: BBa_K797013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K797013/annotation2196003/range2196003/1

https://synbiohub.org/public/igem/BBa_K797013/annotation2196003/range2196003/1
type: Range
persistent identity: range2196003
display id: range2196003
version: 1
direction: direction/reverse
top level: BBa_K797013
start: 2199
end: 2219
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2196002
display id: annotation2196002
version: 1
top level: BBa_K797013
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K797013/annotation2196002/range2196002/1

https://synbiohub.org/public/igem/BBa_K797013/annotation2196002/range2196002/1
type: Range
persistent identity: range2196002
display id: range2196002
version: 1
direction: direction/reverse
top level: BBa_K797013
start: 2101
end: 2120
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2196001
display id: annotation2196001
version: 1
top level: BBa_K797013
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K797013/annotation2196001/range2196001/1

https://synbiohub.org/public/igem/BBa_K797013/annotation2196001/range2196001/1
type: Range
persistent identity: range2196001
display id: range2196001
version: 1
direction: direction/forward
top level: BBa_K797013
start: 157
end: 176
orientation: inline

BsaI Restriction site
type: SequenceAnnotation
persistent identity: annotation2196004
display id: annotation2196004
version: 1
top level: BBa_K797013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K797013/annotation2196004/range2196004/1

https://synbiohub.org/public/igem/BBa_K797013/annotation2196004/range2196004/1
type: Range
persistent identity: range2196004
display id: range2196004
version: 1
direction: direction/reverse
top level: BBa_K797013
start: 2219
end: 2222
orientation: inline

BBa_K563054
type: ComponentDefinition
persistent identity: BBa K563054
display id: BBa_K563054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Vector pYD, and can transfer into nucles of S.cerevisivae
creator: Guo Jing
created: 2011-10-04T11:00:00Z
modified: 2015-05-08T01:12:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _731_
m user id: 0
owner id: 10236
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pYD is a 5.0kb expression vector designed for expression, secretion, and display of proteins on the extracellular surface of S.cerevisivae cells. Features of this vector allow regulated expression, secretion and detection of expressed proteins.
mutable notes: In our project, to make our mutation of Tor2 easier, we separate complete Tor2 into three parts and mutate them independently. So we reconstruct pYD vector to add certain restriction sites for linking mutant Tor2 and original Tor2
mutable provenance: AGA2, GAL1, TRP5 and CEN come from S.cerevisivae. Amppicillin resistance gene and the pUC origin for selection and replication come from E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Amppicillin
sequence annotation: GAL1
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K563054_sequence/1

Amppicillin
type: SequenceAnnotation
persistent identity: annotation2153209
display id: annotation2153209
version: 1
top level: BBa_K563054
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K563054/annotation2153209/range2153209/1

https://synbiohub.org/public/igem/BBa_K563054/annotation2153209/range2153209/1
type: Range
persistent identity: range2153209
display id: range2153209
version: 1
direction: direction/forward
top level: BBa_K563054
start: 2931
end: 3791
orientation: inline

GAL1
type: SequenceAnnotation
persistent identity: annotation2153207
display id: annotation2153207
version: 1
top level: BBa_K563054
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K563054/annotation2153207/range2153207/1

https://synbiohub.org/public/igem/BBa_K563054/annotation2153207/range2153207/1
type: Range
persistent identity: range2153207
display id: range2153207
version: 1
direction: direction/forward
top level: BBa_K563054
start: 1
end: 451
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2153208
display id: annotation2153208
version: 1
top level: BBa_K563054
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K563054/annotation2153208/range2153208/1

https://synbiohub.org/public/igem/BBa_K563054/annotation2153208/range2153208/1
type: Range
persistent identity: range2153208
display id: range2153208
version: 1
direction: direction/forward
top level: BBa_K563054
start: 475
end: 494
orientation: inline

pSB1A2
type: ComponentDefinition
persistent identity: BBa K300988
display id: BBa_K300988
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A2 without Prefix and Suffix
creator: Susanna Zucca, Federica Sampietro, Paolo Magni
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is identical to pSB1A2, but it does not have the Prefix and Suffix. It can be useful to specify the sequence of new vectors constructed with BioBrick parts, such as BBa_K300001 .
mutable notes: No changes have been made to pSB1A2 sequence.
mutable provenance: See pSB1A2 .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: terminator
sequence annotation: VF2
sequence annotation: ORI
sequence annotation: VR
sequence annotation: pBLA (rev)
sequence annotation: rep (pMB1) (rev)
sequence annotation: APr (rev)
sequence: https://synbiohub.org/public/igem/BBa_K300988_sequence/1

terminator
type: SequenceAnnotation
persistent identity: annotation2081416
display id: annotation2081416
version: 1
top level: pSB1A2
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081416/range2081416/1

https://synbiohub.org/public/igem/BBa_K300988/annotation2081416/range2081416/1
type: Range
persistent identity: range2081416
display id: range2081416
version: 1
direction: direction/forward
top level: pSB1A2
start: 2008
end: 2027
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2081415
display id: annotation2081415
version: 1
top level: pSB1A2
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081415/range2081415/1

https://synbiohub.org/public/igem/BBa_K300988/annotation2081415/range2081415/1
type: Range
persistent identity: range2081415
display id: range2081415
version: 1
direction: direction/forward
top level: pSB1A2
start: 1919
end: 1938
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2081412
display id: annotation2081412
version: 1
top level: pSB1A2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081412/range2081412/1

https://synbiohub.org/public/igem/BBa_K300988/annotation2081412/range2081412/1
type: Range
persistent identity: range2081412
display id: range2081412
version: 1
direction: direction/forward
top level: pSB1A2
start: 177
end: 177
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2081410
display id: annotation2081410
version: 1
top level: pSB1A2
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081410/range2081410/1

https://synbiohub.org/public/igem/BBa_K300988/annotation2081410/range2081410/1
type: Range
persistent identity: range2081410
display id: range2081410
version: 1
direction: direction/reverse
top level: pSB1A2
start: 58
end: 77
orientation: inline

pBLA (rev)
type: SequenceAnnotation
persistent identity: annotation2081414
display id: annotation2081414
version: 1
top level: pSB1A2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081414/range2081414/1

https://synbiohub.org/public/igem/BBa_K300988/annotation2081414/range2081414/1
type: Range
persistent identity: range2081414
display id: range2081414
version: 1
direction: direction/reverse
top level: pSB1A2
start: 1831
end: 1831
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2081411
display id: annotation2081411
version: 1
top level: pSB1A2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081411/range2081411/1

https://synbiohub.org/public/igem/BBa_K300988/annotation2081411/range2081411/1
type: Range
persistent identity: range2081411
display id: range2081411
version: 1
direction: direction/reverse
top level: pSB1A2
start: 162
end: 776
orientation: inline

APr (rev)
type: SequenceAnnotation
persistent identity: annotation2081413
display id: annotation2081413
version: 1
top level: pSB1A2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K300988/annotation2081413/range2081413/1

https://synbiohub.org/public/igem/BBa_K300988/annotation2081413/range2081413/1
type: Range
persistent identity: range2081413
display id: range2081413
version: 1
direction: direction/reverse
top level: pSB1A2
start: 936
end: 1796
orientation: inline

BBa_K106670
type: ComponentDefinition
persistent identity: BBa K106670
display id: BBa_K106670
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor, pRS315, 8X LexAOPs Cyc1P, Adht1
creator: Andrew Horwitz
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is an acceptor vector for AarI cloning with 8X Lex operators directly upstream of a Cyc1 promoter. The vector contains the AarI cloning cassette (AD sites, with Adh1 terminator). It is a pRS315 derived vector, for CEN ARS expression in cerevisiae (LEU2 marker).
mutable notes: LexA operator repeats were cloned into the PspOMI site at the 5' end of the Cyc1 promoter. The inner site was broken, thus the operator-promoter-AarI cassette fragment can be subcloned by PspOMI/SacI or Kpn1/SacI digestion into any other pRS series vector.
mutable provenance: This is a derivative of part BBa_K106014, the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ATG start codon
sequence annotation: Cyc1 Promoter
sequence annotation: T3 promoter
sequence annotation: ampicillin resistance marker
sequence annotation: lac promoter
sequence annotation: pBR322 origin
sequence annotation: T7 promoter
sequence annotation: Adh1 terminator
sequence annotation: LEU2 Marker
sequence annotation: 8X LexA Operators
sequence annotation: f1 origin
sequence annotation: D overhang
sequence annotation: A overhang
sequence annotation: ampicillin resistance promoter
sequence annotation: Stop codons in all 3 frames
sequence annotation: lacZalpha reporter
sequence annotation: CEN ARS
sequence: https://synbiohub.org/public/igem/BBa_K106670_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation1999702
display id: annotation1999702
version: 1
top level: BBa_K106670
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999702/range1999702/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999702/range1999702/1
type: Range
persistent identity: range1999702
display id: range1999702
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6547
end: 6549
orientation: inline

Cyc1 Promoter
type: SequenceAnnotation
persistent identity: annotation1990434
display id: annotation1990434
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990434/range1990434/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990434/range1990434/1
type: Range
persistent identity: range1990434
display id: range1990434
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6300
end: 6556
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999701
display id: annotation1999701
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999701/range1999701/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999701/range1999701/1
type: Range
persistent identity: range1999701
display id: range1999701
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6095
end: 6114
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1990432
display id: annotation1990432
version: 1
top level: BBa_K106670
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990432/range1990432/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990432/range1990432/1
type: Range
persistent identity: range1990432
display id: range1990432
version: 1
direction: direction/forward
top level: BBa_K106670
start: 4053
end: 4911
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999700
display id: annotation1999700
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999700/range1999700/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999700/range1999700/1
type: Range
persistent identity: range1999700
display id: range1999700
version: 1
direction: direction/forward
top level: BBa_K106670
start: 5996
end: 6025
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999699
display id: annotation1999699
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999699/range1999699/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999699/range1999699/1
type: Range
persistent identity: range1999699
display id: range1999699
version: 1
direction: direction/forward
top level: BBa_K106670
start: 5068
end: 5687
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999696
display id: annotation1999696
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999696/range1999696/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999696/range1999696/1
type: Range
persistent identity: range1999696
display id: range1999696
version: 1
direction: direction/reverse
top level: BBa_K106670
start: 224
end: 242
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1990437
display id: annotation1990437
version: 1
top level: BBa_K106670
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990437/range1990437/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990437/range1990437/1
type: Range
persistent identity: range1990437
display id: range1990437
version: 1
direction: direction/forward
top level: BBa_K106670
start: 23
end: 216
orientation: inline

LEU2 Marker
type: SequenceAnnotation
persistent identity: annotation1990438
display id: annotation1990438
version: 1
top level: BBa_K106670
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990438/range1990438/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990438/range1990438/1
type: Range
persistent identity: range1990438
display id: range1990438
version: 1
direction: direction/forward
top level: BBa_K106670
start: 1570
end: 2676
orientation: inline

8X LexA Operators
type: SequenceAnnotation
persistent identity: annotation1990433
display id: annotation1990433
version: 1
top level: BBa_K106670
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990433/range1990433/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990433/range1990433/1
type: Range
persistent identity: range1990433
display id: range1990433
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6132
end: 6299
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999698
display id: annotation1999698
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999698/range1999698/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999698/range1999698/1
type: Range
persistent identity: range1999698
display id: range1999698
version: 1
direction: direction/forward
top level: BBa_K106670
start: 427
end: 733
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999693
display id: annotation1999693
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999693/range1999693/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999693/range1999693/1
type: Range
persistent identity: range1999693
display id: range1999693
version: 1
direction: direction/forward
top level: BBa_K106670
start: 1
end: 4
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999695
display id: annotation1999695
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999695/range1999695/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999695/range1999695/1
type: Range
persistent identity: range1999695
display id: range1999695
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6556
end: 6559
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1990435
display id: annotation1990435
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990435/range1990435/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990435/range1990435/1
type: Range
persistent identity: range1990435
display id: range1990435
version: 1
direction: direction/forward
top level: BBa_K106670
start: 3983
end: 4011
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999694
display id: annotation1999694
version: 1
top level: BBa_K106670
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999694/range1999694/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999694/range1999694/1
type: Range
persistent identity: range1999694
display id: range1999694
version: 1
direction: direction/forward
top level: BBa_K106670
start: 11
end: 21
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999697
display id: annotation1999697
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999697/range1999697/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999697/range1999697/1
type: Range
persistent identity: range1999697
display id: range1999697
version: 1
direction: direction/forward
top level: BBa_K106670
start: 246
end: 405
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1990431
display id: annotation1990431
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990431/range1990431/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990431/range1990431/1
type: Range
persistent identity: range1990431
display id: range1990431
version: 1
direction: direction/reverse
top level: BBa_K106670
start: 3403
end: 3921
orientation: inline

BBa_K260001
type: ComponentDefinition
persistent identity: BBa K260001
display id: BBa_K260001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCC2FosMB
creator: Kaj Bernhardt
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _356_
m user id: 0
owner id: 1072
owning group id: 57
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This fosmid backbone was generated by recombineering-mediated transfer of the HincII fragment from BBa_K260016 (inside backbone BBa_K260010) to pCC2FosM (BBa_K260000). This reaction also transferred the blasticidin resistance gene (BsdR), which is now part of this backbone. pCC2FosMB is available with part BBa_K260016 between prefix and suffix.
mutable notes: The insertion site for BsdR was chosen to be upstream of MECP2 exon 1 but still with more than 10 kb of MECP genomic sequence upstream.
mutable provenance: Based on BBa_K260000, so it also contains human MECP2 genomic sequence, but has an insert taken from BBa_K260010.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: prefix
sequence annotation: SpecR
sequence annotation: suffix
sequence annotation: gb3 promoter
sequence annotation: CmR
sequence annotation: BsdR
sequence: https://synbiohub.org/public/igem/BBa_K260001_sequence/1

prefix
type: SequenceAnnotation
persistent identity: annotation2048665
display id: annotation2048665
version: 1
top level: BBa_K260001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260001/annotation2048665/range2048665/1

https://synbiohub.org/public/igem/BBa_K260001/annotation2048665/range2048665/1
type: Range
persistent identity: range2048665
display id: range2048665
version: 1
direction: direction/forward
top level: BBa_K260001
start: 48151
end: 48172
orientation: inline

SpecR
type: SequenceAnnotation
persistent identity: annotation2048667
display id: annotation2048667
version: 1
top level: BBa_K260001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260001/annotation2048667/range2048667/1

https://synbiohub.org/public/igem/BBa_K260001/annotation2048667/range2048667/1
type: Range
persistent identity: range2048667
display id: range2048667
version: 1
direction: direction/forward
top level: BBa_K260001
start: 18108
end: 18782
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2048643
display id: annotation2048643
version: 1
top level: BBa_K260001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260001/annotation2048643/range2048643/1

https://synbiohub.org/public/igem/BBa_K260001/annotation2048643/range2048643/1
type: Range
persistent identity: range2048643
display id: range2048643
version: 1
direction: direction/forward
top level: BBa_K260001
start: 1
end: 21
orientation: inline

gb3 promoter
type: SequenceAnnotation
persistent identity: annotation2048669
display id: annotation2048669
version: 1
top level: BBa_K260001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K260001/annotation2048669/range2048669/1

https://synbiohub.org/public/igem/BBa_K260001/annotation2048669/range2048669/1
type: Range
persistent identity: range2048669
display id: range2048669
version: 1
direction: direction/forward
top level: BBa_K260001
start: 22
end: 115
orientation: inline

CmR
type: SequenceAnnotation
persistent identity: annotation2048666
display id: annotation2048666
version: 1
top level: BBa_K260001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260001/annotation2048666/range2048666/1

https://synbiohub.org/public/igem/BBa_K260001/annotation2048666/range2048666/1
type: Range
persistent identity: range2048666
display id: range2048666
version: 1
direction: direction/reverse
top level: BBa_K260001
start: 22815
end: 23474
orientation: inline

BsdR
type: SequenceAnnotation
persistent identity: annotation2048668
display id: annotation2048668
version: 1
top level: BBa_K260001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260001/annotation2048668/range2048668/1

https://synbiohub.org/public/igem/BBa_K260001/annotation2048668/range2048668/1
type: Range
persistent identity: range2048668
display id: range2048668
version: 1
direction: direction/forward
top level: BBa_K260001
start: 116
end: 514
orientation: inline

BBa_J100117
type: ComponentDefinition
persistent identity: BBa J100117
display id: BBa_J100117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A2 w/ "A" at -32 upstream from RNAII
creator: Erich Baker
created: 2013-07-23T11:00:00Z
modified: 2015-08-31T04:08:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _578_
m user id: 0
owner id: 10666
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a modified version of plasmid pSB1A2 (http://parts.igem.org/Part:pSB1A2). The position 32 bases upstream from the beginning of the RNAII transcript had been changed from a "C" to an "A". Mutations at this position have been shown to affect plasmid copy number in Castagnoli, 1985 (http://www.ncbi.nlm.nih.gov/pubmed/3895161).
mutable notes: Part was created by ligating pSB1A2 iPCR product to annealed oligos with an "N" at the -32 position.
mutable provenance: pSB1A2 used came from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Prefix
sequence annotation: Suffix
sequence annotation: "A"
sequence annotation: APr (Rev)
sequence annotation: rep (pMB1) (rev)
sequence: https://synbiohub.org/public/igem/BBa_J100117_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation2331066
display id: annotation2331066
version: 1
top level: BBa_J100117
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J100117/annotation2331066/range2331066/1

https://synbiohub.org/public/igem/BBa_J100117/annotation2331066/range2331066/1
type: Range
persistent identity: range2331066
display id: range2331066
version: 1
direction: direction/forward
top level: BBa_J100117
start: 2058
end: 2079
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2331065
display id: annotation2331065
version: 1
top level: BBa_J100117
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J100117/annotation2331065/range2331065/1

https://synbiohub.org/public/igem/BBa_J100117/annotation2331065/range2331065/1
type: Range
persistent identity: range2331065
display id: range2331065
version: 1
direction: direction/forward
top level: BBa_J100117
start: 1
end: 21
orientation: inline

"A"
type: SequenceAnnotation
persistent identity: annotation2331069
display id: annotation2331069
version: 1
top level: BBa_J100117
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J100117/annotation2331069/range2331069/1

https://synbiohub.org/public/igem/BBa_J100117/annotation2331069/range2331069/1
type: Range
persistent identity: range2331069
display id: range2331069
version: 1
direction: direction/forward
top level: BBa_J100117
start: 782
end: 782
orientation: inline

APr (Rev)
type: SequenceAnnotation
persistent identity: annotation2331068
display id: annotation2331068
version: 1
top level: BBa_J100117
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J100117/annotation2331068/range2331068/1

https://synbiohub.org/public/igem/BBa_J100117/annotation2331068/range2331068/1
type: Range
persistent identity: range2331068
display id: range2331068
version: 1
direction: direction/reverse
top level: BBa_J100117
start: 957
end: 1827
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2331067
display id: annotation2331067
version: 1
top level: BBa_J100117
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J100117/annotation2331067/range2331067/1

https://synbiohub.org/public/igem/BBa_J100117/annotation2331067/range2331067/1
type: Range
persistent identity: range2331067
display id: range2331067
version: 1
direction: direction/reverse
top level: BBa_J100117
start: 183
end: 797
orientation: inline

BBa_K319033
type: ComponentDefinition
persistent identity: BBa K319033
display id: BBa_K319033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ESV
creator: Afnan Azizi
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
group u list: _437_
m user id: 0
owner id: 7363
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This plasmid can be used to eliminate an upstream PstI restriction site and a downstream BamHI restriction site in a potential BioBrick part.
mutable notes: N/A
mutable provenance: It was obtained by performing a PCR reaction amplifying the pSB1C3 plasmid and using appropriate overhangs containing an NsiI site to combine with the PstI site and a BglII site to combine with the BamHI site.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K319033_sequence/1

BBa_J179001
type: ComponentDefinition
persistent identity: BBa J179001
display id: BBa_J179001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBS2E: Bacillus subtilis vector, lacA integration, MLS resistance
creator: Jara Radeck
created: 2013-12-20T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli.
mutable notes: Compatible with pBs1C and pBs4S in one strain.
mutable provenance: This backbone is a BioBricked version of the B. subtilis vector pAX01. This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox] Reference: [http://www.ncbi.nlm.nih.gov/pubmed/11274134 H??rtl ''et al.'']
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ori
sequence annotation: lacA back
sequence annotation: bla
sequence annotation: erm
sequence annotation: lacA front
sequence: https://synbiohub.org/public/igem/BBa_J179001_sequence/1

ori
type: SequenceAnnotation
persistent identity: annotation2371763
display id: annotation2371763
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371763/range2371763/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371763/range2371763/1
type: Range
persistent identity: range2371763
display id: range2371763
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 1758
end: 2381
orientation: inline

lacA back
type: SequenceAnnotation
persistent identity: annotation2371761
display id: annotation2371761
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371761/range2371761/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371761/range2371761/1
type: Range
persistent identity: range2371761
display id: range2371761
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 124
end: 531
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371762
display id: annotation2371762
version: 1
top level: BBa_J179001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371762/range2371762/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371762/range2371762/1
type: Range
persistent identity: range2371762
display id: range2371762
version: 1
direction: direction/forward
top level: BBa_J179001
start: 747
end: 1607
orientation: inline

erm
type: SequenceAnnotation
persistent identity: annotation2371765
display id: annotation2371765
version: 1
top level: BBa_J179001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371765/range2371765/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371765/range2371765/1
type: Range
persistent identity: range2371765
display id: range2371765
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 4891
end: 5628
orientation: inline

lacA front
type: SequenceAnnotation
persistent identity: annotation2371764
display id: annotation2371764
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371764/range2371764/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371764/range2371764/1
type: Range
persistent identity: range2371764
display id: range2371764
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 4078
end: 4593
orientation: inline

BBa_K1971011
type: ComponentDefinition
persistent identity: BBa K1971011
display id: BBa_K1971011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psb1c3 backbone with BsaI site
creator: KE GUAN YU
created: 2016-10-19T11:00:00Z
modified: 2016-10-20T02:44:34Z
discontinued: false
dominant: false
group u list: _2438_
m user id: 29894
owner id: 29894
owning group id: 9
status: status/Planning
bookmark: false
mutable description: psb1c3 backbone with BsaI site
mutable notes: None
mutable provenance: psb1c3 circulized plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1971011_sequence/1

BBa_J72208
type: ComponentDefinition
persistent identity: BBa J72208
display id: BBa_J72208
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kanamycin resistant BAC with R6K origin
creator: Josh Kittleson
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:08:29Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Kanamycin resistant BAC with intact R6K origin (BBa format). Can be maintained at high copy in strains expressing pir Referred to internally as pBacO.
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72208_sequence/1

pSB8K15FRT
type: ComponentDefinition
persistent identity: BBa K864022
display id: BBa_K864022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: awd
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864022_sequence/1

BBa_K1314015
type: ComponentDefinition
persistent identity: BBa K1314015
display id: BBa_K1314015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Homologous stable integration for nifH promoter T7 expression system Tookit
creator: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1689_
m user id: 0
owner id: 19949
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Homologous stable integration for nifH promoter T7 expression system Tookit
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Biobrick suffix
sequence annotation: K1314003
sequence annotation: Scar
sequence annotation: Biobrick prefix
sequence annotation: K1314010
sequence: https://synbiohub.org/public/igem/BBa_K1314015_sequence/1

Biobrick suffix
type: SequenceAnnotation
persistent identity: annotation2398190
display id: annotation2398190
version: 1
top level: BBa_K1314015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1314015/annotation2398190/range2398190/1

https://synbiohub.org/public/igem/BBa_K1314015/annotation2398190/range2398190/1
type: Range
persistent identity: range2398190
display id: range2398190
version: 1
direction: direction/forward
top level: BBa_K1314015
start: 1
end: 21
orientation: inline

K1314003
type: SequenceAnnotation
persistent identity: annotation2398191
display id: annotation2398191
version: 1
top level: BBa_K1314015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1314015/annotation2398191/range2398191/1

https://synbiohub.org/public/igem/BBa_K1314015/annotation2398191/range2398191/1
type: Range
persistent identity: range2398191
display id: range2398191
version: 1
direction: direction/forward
top level: BBa_K1314015
start: 22
end: 374
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation2398192
display id: annotation2398192
version: 1
top level: BBa_K1314015
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1314015/annotation2398192/range2398192/1

https://synbiohub.org/public/igem/BBa_K1314015/annotation2398192/range2398192/1
type: Range
persistent identity: range2398192
display id: range2398192
version: 1
direction: direction/forward
top level: BBa_K1314015
start: 375
end: 375
orientation: inline

Biobrick prefix
type: SequenceAnnotation
persistent identity: annotation2398194
display id: annotation2398194
version: 1
top level: BBa_K1314015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1314015/annotation2398194/range2398194/1

https://synbiohub.org/public/igem/BBa_K1314015/annotation2398194/range2398194/1
type: Range
persistent identity: range2398194
display id: range2398194
version: 1
direction: direction/forward
top level: BBa_K1314015
start: 2742
end: 2763
orientation: inline

K1314010
type: SequenceAnnotation
persistent identity: annotation2398193
display id: annotation2398193
version: 1
top level: BBa_K1314015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1314015/annotation2398193/range2398193/1

https://synbiohub.org/public/igem/BBa_K1314015/annotation2398193/range2398193/1
type: Range
persistent identity: range2398193
display id: range2398193
version: 1
direction: direction/forward
top level: BBa_K1314015
start: 2538
end: 2741
orientation: inline

BBa_K560013
type: ComponentDefinition
persistent identity: BBa K560013
display id: BBa_K560013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BsaI Golden Gate Assembly No.2
creator: Zhang Chao
created: 2011-10-25T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
group u list: _728_
m user id: 0
owner id: 8553
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The second vector of the Golden gate series (adapted from psb1c3) This vector expedites golden gate cloning with BBa_K560012, BBa_K560014 and BBa_K560015. To make this set of vector compatible with the standard biobricks, we have maintained PstI and XbaI recognition sites. Flanking these two sites are the cleavage and recognition sites of BsaI, a kind of Type IIS restriction endonuclease. This kind of restriction endonucleases cleaves outside their recognition sites thus creating different sticky ends. If you want to assemble four biobricks at a time, you can put the biobricks in order into corresponding vector by the digestion of PstI and Xbal. Then mix these assembled vectors and a backbone (PFUS4 from TALEN golden gate kit). Under the effect of ligase and Bsal, these biobricks will have sticky ends nose to tails and ligate in order in a single reaction.
mutable notes: No
mutable provenance: Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting>>Nucleic Acids Research, 2011, Vol. 39, No. 12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Prefix
sequence annotation: BsaI
sequence annotation: XbaI
sequence annotation: Suffix
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_K560013_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation2160715
display id: annotation2160715
version: 1
top level: BBa_K560013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K560013/annotation2160715/range2160715/1

https://synbiohub.org/public/igem/BBa_K560013/annotation2160715/range2160715/1
type: Range
persistent identity: range2160715
display id: range2160715
version: 1
direction: direction/forward
top level: BBa_K560013
start: 1
end: 24
orientation: inline

BsaI
type: SequenceAnnotation
persistent identity: annotation2160718
display id: annotation2160718
version: 1
top level: BBa_K560013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560013/annotation2160718/range2160718/1

https://synbiohub.org/public/igem/BBa_K560013/annotation2160718/range2160718/1
type: Range
persistent identity: range2160718
display id: range2160718
version: 1
direction: direction/forward
top level: BBa_K560013
start: 14
end: 19
orientation: inline

BsaI
type: SequenceAnnotation
persistent identity: annotation2160719
display id: annotation2160719
version: 1
top level: BBa_K560013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560013/annotation2160719/range2160719/1

https://synbiohub.org/public/igem/BBa_K560013/annotation2160719/range2160719/1
type: Range
persistent identity: range2160719
display id: range2160719
version: 1
direction: direction/forward
top level: BBa_K560013
start: 2062
end: 2067
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2160720
display id: annotation2160720
version: 1
top level: BBa_K560013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560013/annotation2160720/range2160720/1

https://synbiohub.org/public/igem/BBa_K560013/annotation2160720/range2160720/1
type: Range
persistent identity: range2160720
display id: range2160720
version: 1
direction: direction/forward
top level: BBa_K560013
start: 2073
end: 2078
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2160716
display id: annotation2160716
version: 1
top level: BBa_K560013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K560013/annotation2160716/range2160716/1

https://synbiohub.org/public/igem/BBa_K560013/annotation2160716/range2160716/1
type: Range
persistent identity: range2160716
display id: range2160716
version: 1
direction: direction/forward
top level: BBa_K560013
start: 2058
end: 2080
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2160717
display id: annotation2160717
version: 1
top level: BBa_K560013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560013/annotation2160717/range2160717/1

https://synbiohub.org/public/igem/BBa_K560013/annotation2160717/range2160717/1
type: Range
persistent identity: range2160717
display id: range2160717
version: 1
direction: direction/forward
top level: BBa_K560013
start: 3
end: 8
orientation: inline

BBa_K914012
type: ComponentDefinition
persistent identity: BBa K914012
display id: BBa_K914012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A2 with 1 amber mutation
creator: Jean Cury
created: 2012-10-04T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
group u list: _1179_
m user id: 0
owner id: 13470
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB1A2 have a double mutation in the 80th serine (TCC->TAG) of the APr gene. This plasmid need a tRNA amber suppressor to express this gene. So in case of horizontal gene transfer this gene won't be expressed and then the plasmid will be lost more easily.
mutable notes: 2 oligo mutated on 2 positions were used for this purpose.
mutable provenance: This gene is mutated by the PCR quick change mutagenesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K914012_sequence/1

BBa_K1137004
type: ComponentDefinition
persistent identity: BBa K1137004
display id: BBa_K1137004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB3C7
creator: Matthew Deyell
created: 2013-10-03T11:00:00Z
modified: 2015-05-08T01:09:21Z
discontinued: false
dominant: false
group u list: _1449_
m user id: 0
owner id: 15098
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3C7 ??? contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with P15A replicon with a copy number of 10-12 per cell. The cat gene has been maintained conveying chloramphenicol resistance.
mutable notes: Designed to be co-expressed with pSB9S1, pSB10K1, and/or pSB6A3
mutable provenance: Sequence comes from pSB1C3 with the ori and resistance changed form that of pACYC.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Prefix
sequence annotation: Terminator
sequence annotation: CAT
sequence annotation: stem loop
sequence annotation: mRFP
sequence annotation: p15A oriR
sequence annotation: Suffix
sequence: https://synbiohub.org/public/igem/BBa_K1137004_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation2367788
display id: annotation2367788
version: 1
top level: BBa_K1137004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1137004/annotation2367788/range2367788/1

https://synbiohub.org/public/igem/BBa_K1137004/annotation2367788/range2367788/1
type: Range
persistent identity: range2367788
display id: range2367788
version: 1
direction: direction/forward
top level: BBa_K1137004
start: 1
end: 19
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2367797
display id: annotation2367797
version: 1
top level: BBa_K1137004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137004/annotation2367797/range2367797/1

https://synbiohub.org/public/igem/BBa_K1137004/annotation2367797/range2367797/1
type: Range
persistent identity: range2367797
display id: range2367797
version: 1
direction: direction/forward
top level: BBa_K1137004
start: 1226
end: 1266
orientation: inline

CAT
type: SequenceAnnotation
persistent identity: annotation2367799
display id: annotation2367799
version: 1
top level: BBa_K1137004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1137004/annotation2367799/range2367799/1

https://synbiohub.org/public/igem/BBa_K1137004/annotation2367799/range2367799/1
type: Range
persistent identity: range2367799
display id: range2367799
version: 1
direction: direction/forward
top level: BBa_K1137004
start: 2478
end: 3137
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation2367793
display id: annotation2367793
version: 1
top level: BBa_K1137004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137004/annotation2367793/range2367793/1

https://synbiohub.org/public/igem/BBa_K1137004/annotation2367793/range2367793/1
type: Range
persistent identity: range2367793
display id: range2367793
version: 1
direction: direction/forward
top level: BBa_K1137004
start: 1057
end: 1076
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2367800
display id: annotation2367800
version: 1
top level: BBa_K1137004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137004/annotation2367800/range2367800/1

https://synbiohub.org/public/igem/BBa_K1137004/annotation2367800/range2367800/1
type: Range
persistent identity: range2367800
display id: range2367800
version: 1
direction: direction/forward
top level: BBa_K1137004
start: 3367
end: 3438
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2367795
display id: annotation2367795
version: 1
top level: BBa_K1137004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137004/annotation2367795/range2367795/1

https://synbiohub.org/public/igem/BBa_K1137004/annotation2367795/range2367795/1
type: Range
persistent identity: range2367795
display id: range2367795
version: 1
direction: direction/forward
top level: BBa_K1137004
start: 1137
end: 1205
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2367789
display id: annotation2367789
version: 1
top level: BBa_K1137004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1137004/annotation2367789/range2367789/1

https://synbiohub.org/public/igem/BBa_K1137004/annotation2367789/range2367789/1
type: Range
persistent identity: range2367789
display id: range2367789
version: 1
direction: direction/forward
top level: BBa_K1137004
start: 248
end: 925
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2367801
display id: annotation2367801
version: 1
top level: BBa_K1137004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137004/annotation2367801/range2367801/1

https://synbiohub.org/public/igem/BBa_K1137004/annotation2367801/range2367801/1
type: Range
persistent identity: range2367801
display id: range2367801
version: 1
direction: direction/forward
top level: BBa_K1137004
start: 3459
end: 3536
orientation: inline

p15A oriR
type: SequenceAnnotation
persistent identity: annotation2367798
display id: annotation2367798
version: 1
top level: BBa_K1137004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1137004/annotation2367798/range2367798/1

https://synbiohub.org/public/igem/BBa_K1137004/annotation2367798/range2367798/1
type: Range
persistent identity: range2367798
display id: range2367798
version: 1
direction: direction/forward
top level: BBa_K1137004
start: 1288
end: 2112
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2367794
display id: annotation2367794
version: 1
top level: BBa_K1137004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1137004/annotation2367794/range2367794/1

https://synbiohub.org/public/igem/BBa_K1137004/annotation2367794/range2367794/1
type: Range
persistent identity: range2367794
display id: range2367794
version: 1
direction: direction/forward
top level: BBa_K1137004
start: 1092
end: 1111
orientation: inline

BBa_K797011
type: ComponentDefinition
persistent identity: BBa K797011
display id: BBa_K797011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid include T7 promoter, Histag and R9 peptide sequence
creator: Tomohiro Nobeyama
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1053_
m user id: 0
owner id: 11588
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This plasmid has modified T7 promoter,RBS,His tag and nine Argenine signal peptide(R9 peptide). R9 peptide cause endocytosis of mammalian and plant cells and make them take not only R9 peptide but also Proteins combined to this peptide. you can correct and purify products of this promoter by using anti-His tag antibody.
mutable provenance: This part is synthesized artificially
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: R9
sequence annotation: RBS
sequence annotation: prefix
sequence annotation: T7
sequence annotation: 6His
sequence annotation: suffix
sequence: https://synbiohub.org/public/igem/BBa_K797011_sequence/1

R9
type: SequenceAnnotation
persistent identity: annotation2195803
display id: annotation2195803
version: 1
top level: BBa_K797011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K797011/annotation2195803/range2195803/1

https://synbiohub.org/public/igem/BBa_K797011/annotation2195803/range2195803/1
type: Range
persistent identity: range2195803
display id: range2195803
version: 1
direction: direction/forward
top level: BBa_K797011
start: 93
end: 119
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2195801
display id: annotation2195801
version: 1
top level: BBa_K797011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K797011/annotation2195801/range2195801/1

https://synbiohub.org/public/igem/BBa_K797011/annotation2195801/range2195801/1
type: Range
persistent identity: range2195801
display id: range2195801
version: 1
direction: direction/forward
top level: BBa_K797011
start: 54
end: 65
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2195799
display id: annotation2195799
version: 1
top level: BBa_K797011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K797011/annotation2195799/range2195799/1

https://synbiohub.org/public/igem/BBa_K797011/annotation2195799/range2195799/1
type: Range
persistent identity: range2195799
display id: range2195799
version: 1
direction: direction/forward
top level: BBa_K797011
start: 1
end: 22
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2195800
display id: annotation2195800
version: 1
top level: BBa_K797011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K797011/annotation2195800/range2195800/1

https://synbiohub.org/public/igem/BBa_K797011/annotation2195800/range2195800/1
type: Range
persistent identity: range2195800
display id: range2195800
version: 1
direction: direction/forward
top level: BBa_K797011
start: 23
end: 42
orientation: inline

6His
type: SequenceAnnotation
persistent identity: annotation2195802
display id: annotation2195802
version: 1
top level: BBa_K797011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K797011/annotation2195802/range2195802/1

https://synbiohub.org/public/igem/BBa_K797011/annotation2195802/range2195802/1
type: Range
persistent identity: range2195802
display id: range2195802
version: 1
direction: direction/forward
top level: BBa_K797011
start: 75
end: 92
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2195804
display id: annotation2195804
version: 1
top level: BBa_K797011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K797011/annotation2195804/range2195804/1

https://synbiohub.org/public/igem/BBa_K797011/annotation2195804/range2195804/1
type: Range
persistent identity: range2195804
display id: range2195804
version: 1
direction: direction/forward
top level: BBa_K797011
start: 121
end: 141
orientation: inline

BBa_K1641999
type: ComponentDefinition
persistent identity: BBa K1641999
display id: BBa_K1641999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAUR135 shuttle vector RFC10 optimized
creator: Chunyang Ni
created: 2015-09-10T11:00:00Z
modified: 2015-09-18T12:55:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2058_
m user id: 23335
owner id: 23335
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pAUR135 RFC25 is a modified shuttle vector including DNA sequence to remove the vector region containing selectable marker AUR1-C from transformants of S.cerevisiae by this vector which allows repeatedly transformation. With optimization, K1641999 can be applied on RFC standard.
mutable notes: mutation and MCS modification
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: GAL10 promoter
sequence annotation: ori
sequence annotation: AmpR
sequence annotation: Lac operator
sequence annotation: AurR
sequence: https://synbiohub.org/public/igem/BBa_K1641999_sequence/1

GAL10 promoter
type: SequenceAnnotation
persistent identity: annotation2452346
display id: annotation2452346
version: 1
top level: BBa_K1641999
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1641999/annotation2452346/range2452346/1

https://synbiohub.org/public/igem/BBa_K1641999/annotation2452346/range2452346/1
type: Range
persistent identity: range2452346
display id: range2452346
version: 1
direction: direction/forward
top level: BBa_K1641999
start: 4434
end: 5098
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2452358
display id: annotation2452358
version: 1
top level: BBa_K1641999
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1641999/annotation2452358/range2452358/1

https://synbiohub.org/public/igem/BBa_K1641999/annotation2452358/range2452358/1
type: Range
persistent identity: range2452358
display id: range2452358
version: 1
direction: direction/forward
top level: BBa_K1641999
start: 1513
end: 2100
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2452357
display id: annotation2452357
version: 1
top level: BBa_K1641999
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1641999/annotation2452357/range2452357/1

https://synbiohub.org/public/igem/BBa_K1641999/annotation2452357/range2452357/1
type: Range
persistent identity: range2452357
display id: range2452357
version: 1
direction: direction/forward
top level: BBa_K1641999
start: 481
end: 1342
orientation: inline

Lac operator
type: SequenceAnnotation
persistent identity: annotation2452347
display id: annotation2452347
version: 1
top level: BBa_K1641999
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1641999/annotation2452347/range2452347/1

https://synbiohub.org/public/igem/BBa_K1641999/annotation2452347/range2452347/1
type: Range
persistent identity: range2452347
display id: range2452347
version: 1
direction: direction/forward
top level: BBa_K1641999
start: 2424
end: 2478
orientation: inline

AurR
type: SequenceAnnotation
persistent identity: annotation2452339
display id: annotation2452339
version: 1
top level: BBa_K1641999
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1641999/annotation2452339/range2452339/1

https://synbiohub.org/public/igem/BBa_K1641999/annotation2452339/range2452339/1
type: Range
persistent identity: range2452339
display id: range2452339
version: 1
direction: direction/forward
top level: BBa_K1641999
start: 2822
end: 4027
orientation: inline

pTG262-BB
type: ComponentDefinition
persistent identity: BBa I742123
display id: BBa_I742123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Multi-host vector pTG262 converted to BioBrick vector
creator: Sarah Hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: biobricked version of pTG262 Plasmid has a multi host replication origin and is competant in range of gram positive and gram negative bacteria.
mutable notes: n/a
mutable provenance: We kindly thank Dr Mike Gasson & Dr. Claire Shearman, of the Institute of Food Research in Norwich for generously donating the pTG262 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick prefix
sequence annotation: cat
sequence annotation: BioBrick suffix
sequence annotation: neo
sequence annotation: repA
sequence: https://synbiohub.org/public/igem/BBa_I742123_sequence/1

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1974398
display id: annotation1974398
version: 1
top level: pTG262-BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974398/range1974398/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974398/range1974398/1
type: Range
persistent identity: range1974398
display id: range1974398
version: 1
direction: direction/forward
top level: pTG262-BB
start: 5543
end: 5564
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation1974397
display id: annotation1974397
version: 1
top level: pTG262-BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974397/range1974397/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974397/range1974397/1
type: Range
persistent identity: range1974397
display id: range1974397
version: 1
direction: direction/forward
top level: pTG262-BB
start: 4444
end: 5094
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1974394
display id: annotation1974394
version: 1
top level: pTG262-BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974394/range1974394/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974394/range1974394/1
type: Range
persistent identity: range1974394
display id: range1974394
version: 1
direction: direction/forward
top level: pTG262-BB
start: 1
end: 21
orientation: inline

neo
type: SequenceAnnotation
persistent identity: annotation1974396
display id: annotation1974396
version: 1
top level: pTG262-BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974396/range1974396/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974396/range1974396/1
type: Range
persistent identity: range1974396
display id: range1974396
version: 1
direction: direction/forward
top level: pTG262-BB
start: 3149
end: 3919
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation1974395
display id: annotation1974395
version: 1
top level: pTG262-BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974395/range1974395/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974395/range1974395/1
type: Range
persistent identity: range1974395
display id: range1974395
version: 1
direction: direction/forward
top level: pTG262-BB
start: 1724
end: 2431
orientation: inline

pPICZ&#945
type: ComponentDefinition
persistent identity: BBa K805020
display id: BBa_K805020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pPICZα
creator: Tang Yong
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1062_
m user id: 0
owner id: 11895
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: test
mutable notes: test
mutable provenance: yes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: protein
sequence annotation: start
sequence annotation: plasmid backbone
sequence: https://synbiohub.org/public/igem/BBa_K805020_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2200594
display id: annotation2200594
version: 1
top level: pPICZ&#945
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K805020/annotation2200594/range2200594/1

https://synbiohub.org/public/igem/BBa_K805020/annotation2200594/range2200594/1
type: Range
persistent identity: range2200594
display id: range2200594
version: 1
direction: direction/forward
top level: pPICZ&#945
start: 1247
end: 2446
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2200512
display id: annotation2200512
version: 1
top level: pPICZ&#945
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K805020/annotation2200512/range2200512/1

https://synbiohub.org/public/igem/BBa_K805020/annotation2200512/range2200512/1
type: Range
persistent identity: range2200512
display id: range2200512
version: 1
direction: direction/forward
top level: pPICZ&#945
start: 1214
end: 1246
orientation: inline

plasmid backbone
type: SequenceAnnotation
persistent identity: annotation2200603
display id: annotation2200603
version: 1
top level: pPICZ&#945
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K805020/annotation2200603/range2200603/1

https://synbiohub.org/public/igem/BBa_K805020/annotation2200603/range2200603/1
type: Range
persistent identity: range2200603
display id: range2200603
version: 1
direction: direction/forward
top level: pPICZ&#945
start: 1
end: 1213
orientation: inline

plasmid backbone
type: SequenceAnnotation
persistent identity: annotation2200604
display id: annotation2200604
version: 1
top level: pPICZ&#945
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K805020/annotation2200604/range2200604/1

https://synbiohub.org/public/igem/BBa_K805020/annotation2200604/range2200604/1
type: Range
persistent identity: range2200604
display id: range2200604
version: 1
direction: direction/forward
top level: pPICZ&#945
start: 2447
end: 4777
orientation: inline

pSB1T3
type: ComponentDefinition
persistent identity: pSB1T3
display id: pSB1T3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Austin Che
created: 2008-09-07T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.
mutable notes: The ampicillin resistance marker needed to be eliminated.
mutable provenance: Austin Che constructed this plamid from pSB1AT3-P1010.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Bind Site 5
sequence annotation: BioBrick suffix
sequence annotation: two base pair AC deletion
sequence annotation: Stem loop
sequence annotation: E. coli his operon terminator
sequence annotation: C->T mutation
sequence annotation: Bind Site 1
sequence annotation: Verifiction forward (VF2) primer binding site
sequence annotation: Promoter P2
sequence annotation: ORI
sequence annotation: Promoter P1
sequence annotation: Bind Site 3
sequence annotation: Bind Site 4
sequence annotation: BioBrick prefix
sequence annotation: rep (pMB1)
sequence annotation: end of terminator
sequence annotation: Tetracycline resistance
sequence annotation: Bind Site 2
sequence annotation: Verification reverse (VR) primer binding site
sequence: https://synbiohub.org/public/igem/pSB1T3_sequence/1

Bind Site 5
type: SequenceAnnotation
persistent identity: annotation1975549
display id: annotation1975549
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975549/range1975549/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975549/range1975549/1
type: Range
persistent identity: range1975549
display id: range1975549
version: 1
direction: direction/forward
top level: pSB1T3
start: 1121
end: 1124
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975537
display id: annotation1975537
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975537/range1975537/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975537/range1975537/1
type: Range
persistent identity: range1975537
display id: range1975537
version: 1
direction: direction/forward
top level: pSB1T3
start: 1
end: 21
orientation: inline

two base pair AC deletion
type: SequenceAnnotation
persistent identity: annotation1975536
display id: annotation1975536
version: 1
top level: pSB1T3
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975536/range1975536/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975536/range1975536/1
type: Range
persistent identity: range1975536
display id: range1975536
version: 1
direction: direction/forward
top level: pSB1T3
start: 34
end: 35
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975538
display id: annotation1975538
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975538/range1975538/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975538/range1975538/1
type: Range
persistent identity: range1975538
display id: range1975538
version: 1
direction: direction/forward
top level: pSB1T3
start: 30
end: 64
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1975539
display id: annotation1975539
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975539/range1975539/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975539/range1975539/1
type: Range
persistent identity: range1975539
display id: range1975539
version: 1
direction: direction/forward
top level: pSB1T3
start: 22
end: 93
orientation: inline

C->T mutation
type: SequenceAnnotation
persistent identity: annotation1975551
display id: annotation1975551
version: 1
top level: pSB1T3
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975551/range1975551/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975551/range1975551/1
type: Range
persistent identity: range1975551
display id: range1975551
version: 1
direction: direction/forward
top level: pSB1T3
start: 2175
end: 2175
orientation: inline

Bind Site 1
type: SequenceAnnotation
persistent identity: annotation1975544
display id: annotation1975544
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975544/range1975544/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975544/range1975544/1
type: Range
persistent identity: range1975544
display id: range1975544
version: 1
direction: direction/forward
top level: pSB1T3
start: 1065
end: 1068
orientation: inline

Verifiction forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975552
display id: annotation1975552
version: 1
top level: pSB1T3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975552/range1975552/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975552/range1975552/1
type: Range
persistent identity: range1975552
display id: range1975552
version: 1
direction: direction/forward
top level: pSB1T3
start: 2324
end: 2343
orientation: inline

Promoter P2
type: SequenceAnnotation
persistent identity: annotation1975547
display id: annotation1975547
version: 1
top level: pSB1T3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975547/range1975547/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975547/range1975547/1
type: Range
persistent identity: range1975547
display id: range1975547
version: 1
direction: direction/forward
top level: pSB1T3
start: 1084
end: 1090
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1975541
display id: annotation1975541
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975541/range1975541/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975541/range1975541/1
type: Range
persistent identity: range1975541
display id: range1975541
version: 1
direction: direction/forward
top level: pSB1T3
start: 276
end: 276
orientation: inline

Promoter P1
type: SequenceAnnotation
persistent identity: annotation1975543
display id: annotation1975543
version: 1
top level: pSB1T3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975543/range1975543/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975543/range1975543/1
type: Range
persistent identity: range1975543
display id: range1975543
version: 1
direction: direction/reverse
top level: pSB1T3
start: 1068
end: 1074
orientation: inline

Bind Site 3
type: SequenceAnnotation
persistent identity: annotation1975546
display id: annotation1975546
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975546/range1975546/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975546/range1975546/1
type: Range
persistent identity: range1975546
display id: range1975546
version: 1
direction: direction/forward
top level: pSB1T3
start: 1094
end: 1097
orientation: inline

Bind Site 4
type: SequenceAnnotation
persistent identity: annotation1975548
display id: annotation1975548
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975548/range1975548/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975548/range1975548/1
type: Range
persistent identity: range1975548
display id: range1975548
version: 1
direction: direction/forward
top level: pSB1T3
start: 1108
end: 1111
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1975555
display id: annotation1975555
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975555/range1975555/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975555/range1975555/1
type: Range
persistent identity: range1975555
display id: range1975555
version: 1
direction: direction/forward
top level: pSB1T3
start: 2442
end: 2463
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975554
display id: annotation1975554
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975554/range1975554/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975554/range1975554/1
type: Range
persistent identity: range1975554
display id: range1975554
version: 1
direction: direction/reverse
top level: pSB1T3
start: 2413
end: 2432
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1975542
display id: annotation1975542
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975542/range1975542/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975542/range1975542/1
type: Range
persistent identity: range1975542
display id: range1975542
version: 1
direction: direction/reverse
top level: pSB1T3
start: 261
end: 875
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1975553
display id: annotation1975553
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975553/range1975553/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975553/range1975553/1
type: Range
persistent identity: range1975553
display id: range1975553
version: 1
direction: direction/reverse
top level: pSB1T3
start: 2400
end: 2403
orientation: inline

Tetracycline resistance
type: SequenceAnnotation
persistent identity: annotation1975550
display id: annotation1975550
version: 1
top level: pSB1T3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975550/range1975550/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975550/range1975550/1
type: Range
persistent identity: range1975550
display id: range1975550
version: 1
direction: direction/forward
top level: pSB1T3
start: 1127
end: 2317
orientation: inline

Bind Site 2
type: SequenceAnnotation
persistent identity: annotation1975545
display id: annotation1975545
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975545/range1975545/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975545/range1975545/1
type: Range
persistent identity: range1975545
display id: range1975545
version: 1
direction: direction/forward
top level: pSB1T3
start: 1080
end: 1083
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975540
display id: annotation1975540
version: 1
top level: pSB1T3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975540/range1975540/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975540/range1975540/1
type: Range
persistent identity: range1975540
display id: range1975540
version: 1
direction: direction/reverse
top level: pSB1T3
start: 157
end: 176
orientation: inline

BBa_K1639013
type: ComponentDefinition
persistent identity: BBa K1639013
display id: BBa_K1639013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pColA-KanR-dTer
creator: Mustafa Yılmaz
created: 2015-09-14T11:00:00Z
modified: 2015-09-21T06:36:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2056_
m user id: 24789
owner id: 20835
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: A
mutable notes: C
mutable provenance: B
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: suffix
sequence annotation: KanR
sequence annotation: double terminator (B0010-B0012)
sequence annotation: prefix
sequence annotation: Amp promoter
sequence annotation: ColA ori
sequence: https://synbiohub.org/public/igem/BBa_K1639013_sequence/1

suffix
type: SequenceAnnotation
persistent identity: annotation2476438
display id: annotation2476438
version: 1
top level: BBa_K1639013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476438/range2476438/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476438/range2476438/1
type: Range
persistent identity: range2476438
display id: range2476438
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 1925
end: 1944
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2476436
display id: annotation2476436
version: 1
top level: BBa_K1639013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476436/range2476436/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476436/range2476436/1
type: Range
persistent identity: range2476436
display id: range2476436
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 862
end: 1677
orientation: inline

double terminator (B0010-B0012)
type: SequenceAnnotation
persistent identity: annotation2476437
display id: annotation2476437
version: 1
top level: BBa_K1639013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476437/range2476437/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476437/range2476437/1
type: Range
persistent identity: range2476437
display id: range2476437
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 1711
end: 1839
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2476433
display id: annotation2476433
version: 1
top level: BBa_K1639013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476433/range2476433/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476433/range2476433/1
type: Range
persistent identity: range2476433
display id: range2476433
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 1
end: 21
orientation: inline

Amp promoter
type: SequenceAnnotation
persistent identity: annotation2476435
display id: annotation2476435
version: 1
top level: BBa_K1639013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476435/range2476435/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476435/range2476435/1
type: Range
persistent identity: range2476435
display id: range2476435
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 770
end: 861
orientation: inline

ColA ori
type: SequenceAnnotation
persistent identity: annotation2476434
display id: annotation2476434
version: 1
top level: BBa_K1639013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476434/range2476434/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476434/range2476434/1
type: Range
persistent identity: range2476434
display id: range2476434
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 117
end: 752
orientation: inline

BBa_K249005
type: ComponentDefinition
persistent identity: BBa K249005
display id: BBa_K249005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C-terminal Arginine Fusion Vector
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pSB1A3 with an C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon)
mutable notes: Standard
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K249005_sequence/1

pSBBs1C
type: ComponentDefinition
persistent identity: BBa K823023
display id: BBa_K823023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs1C: Empty backbone for integration into Bacillus subtilis amyE locus
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the amyE locus and can be selected with Chloramphenicol (cat gene). It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1662. This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR.
mutable provenance: This backbone is an igem compatible version of the Bacillus vector pDG1662
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: amyE
sequence annotation: cat
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_K823023_sequence/1

amyE
type: SequenceAnnotation
persistent identity: annotation2180237
display id: annotation2180237
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180237/range2180237/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180237/range2180237/1
type: Range
persistent identity: range2180237
display id: range2180237
version: 1
direction: direction/forward
top level: pSBBs1C
start: 1379
end: 2407
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2180239
display id: annotation2180239
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180239/range2180239/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180239/range2180239/1
type: Range
persistent identity: range2180239
display id: range2180239
version: 1
direction: direction/forward
top level: pSBBs1C
start: 5546
end: 6066
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2180236
display id: annotation2180236
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180236/range2180236/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180236/range2180236/1
type: Range
persistent identity: range2180236
display id: range2180236
version: 1
direction: direction/forward
top level: pSBBs1C
start: 358
end: 1008
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180238
display id: annotation2180238
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180238/range2180238/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180238/range2180238/1
type: Range
persistent identity: range2180238
display id: range2180238
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 4361
end: 5221
orientation: inline

BBa_K1480013
type: ComponentDefinition
persistent identity: BBa K1480013
display id: BBa_K1480013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB3C5 + MID TALEN (K1480001) Target site
creator: Roberto Alejandro Valderrama Alfaro
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1860_
m user id: 0
owner id: 19018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Backbone vector of pSB3C5 that contains a modification with a TALEN target site on the center of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains a single TALEN target site (MID).
mutable notes: Modifications where inserted by PCR on both directions due to TALEN specificity.
mutable provenance: Derived from pSB3C5 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1480013_sequence/1

BBa_K1470006
type: ComponentDefinition
persistent identity: BBa K1470006
display id: BBa_K1470006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMIG
creator: Dennis Zimmer
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:39Z
discontinued: false
dominant: false
group u list: _1849_
m user id: 0
owner id: 20942
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Template pMIG
mutable notes: standardization
mutable provenance: Toolbox Institute of Biology II / BIOSS Center for Biological Signalling Studies Albert-Ludwigs-Universit??t Freiburg Germany
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1470006_sequence/1

BBa_K1795400
type: ComponentDefinition
persistent identity: BBa K1795400
display id: BBa_K1795400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Truncated pSB1C3 with no Prefix or Suffix
creator: Joseph L Maniaci
created: 2015-09-17T11:00:00Z
modified: 2015-09-17T11:04:43Z
discontinued: false
dominant: false
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a truncated version of pSB1C3 which does not contain a prefix or suffix. It is made to be used as a construction intermediate for the creation of new plasmid backbones which want to insert additional regions at either end of the backbone before replacing the prefix and suffix at either end of the backbone. It is used in K1795023.
mutable notes: We removed the prefix and suffix from pSB1C3 so we could add additional regions on either end of the backbone before replacing the prefix and suffix.
mutable provenance: iGEM Parts Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1795400_sequence/1

BBa_J119046
type: ComponentDefinition
persistent identity: BBa J119046
display id: BBa_J119046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1K8
creator: A. Malcolm Campbell and Todd Eckdahl
created: 2012-01-23T12:00:00Z
modified: 2015-08-31T04:08:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Insulated vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix.
mutable notes: Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.
mutable provenance: de nove DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR primer
sequence annotation: B0010 terminator
sequence annotation: pMB1 replication origin
sequence annotation: Suffix
sequence annotation: B0025 terminator
sequence annotation: Prefix
sequence annotation: stuffer
sequence annotation: VF2 primer
sequence annotation: Kanamycin resistance gene
sequence: https://synbiohub.org/public/igem/BBa_J119046_sequence/1

VR primer
type: SequenceAnnotation
persistent identity: annotation2169689
display id: annotation2169689
version: 1
top level: BBa_J119046
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119046/annotation2169689/range2169689/1

https://synbiohub.org/public/igem/BBa_J119046/annotation2169689/range2169689/1
type: Range
persistent identity: range2169689
display id: range2169689
version: 1
direction: direction/reverse
top level: BBa_J119046
start: 208
end: 227
orientation: inline

B0010 terminator
type: SequenceAnnotation
persistent identity: annotation2169692
display id: annotation2169692
version: 1
top level: BBa_J119046
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J119046/annotation2169692/range2169692/1

https://synbiohub.org/public/igem/BBa_J119046/annotation2169692/range2169692/1
type: Range
persistent identity: range2169692
display id: range2169692
version: 1
direction: direction/forward
top level: BBa_J119046
start: 2138
end: 2215
orientation: inline

pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation2169694
display id: annotation2169694
version: 1
top level: BBa_J119046
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119046/annotation2169694/range2169694/1

https://synbiohub.org/public/igem/BBa_J119046/annotation2169694/range2169694/1
type: Range
persistent identity: range2169694
display id: range2169694
version: 1
direction: direction/forward
top level: BBa_J119046
start: 310
end: 923
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2169687
display id: annotation2169687
version: 1
top level: BBa_J119046
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119046/annotation2169687/range2169687/1

https://synbiohub.org/public/igem/BBa_J119046/annotation2169687/range2169687/1
type: Range
persistent identity: range2169687
display id: range2169687
version: 1
direction: direction/forward
top level: BBa_J119046
start: 1
end: 21
orientation: inline

B0025 terminator
type: SequenceAnnotation
persistent identity: annotation2169691
display id: annotation2169691
version: 1
top level: BBa_J119046
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J119046/annotation2169691/range2169691/1

https://synbiohub.org/public/igem/BBa_J119046/annotation2169691/range2169691/1
type: Range
persistent identity: range2169691
display id: range2169691
version: 1
direction: direction/reverse
top level: BBa_J119046
start: 22
end: 205
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2169688
display id: annotation2169688
version: 1
top level: BBa_J119046
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119046/annotation2169688/range2169688/1

https://synbiohub.org/public/igem/BBa_J119046/annotation2169688/range2169688/1
type: Range
persistent identity: range2169688
display id: range2169688
version: 1
direction: direction/forward
top level: BBa_J119046
start: 2256
end: 2277
orientation: inline

stuffer
type: SequenceAnnotation
persistent identity: annotation2169693
display id: annotation2169693
version: 1
top level: BBa_J119046
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119046/annotation2169693/range2169693/1

https://synbiohub.org/public/igem/BBa_J119046/annotation2169693/range2169693/1
type: Range
persistent identity: range2169693
display id: range2169693
version: 1
direction: direction/forward
top level: BBa_J119046
start: 2278
end: 2289
orientation: inline

VF2 primer
type: SequenceAnnotation
persistent identity: annotation2169690
display id: annotation2169690
version: 1
top level: BBa_J119046
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119046/annotation2169690/range2169690/1

https://synbiohub.org/public/igem/BBa_J119046/annotation2169690/range2169690/1
type: Range
persistent identity: range2169690
display id: range2169690
version: 1
direction: direction/forward
top level: BBa_J119046
start: 2118
end: 2137
orientation: inline

Kanamycin resistance gene
type: SequenceAnnotation
persistent identity: annotation2169695
display id: annotation2169695
version: 1
top level: BBa_J119046
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J119046/annotation2169695/range2169695/1

https://synbiohub.org/public/igem/BBa_J119046/annotation2169695/range2169695/1
type: Range
persistent identity: range2169695
display id: range2169695
version: 1
direction: direction/forward
top level: BBa_J119046
start: 1148
end: 1963
orientation: inline

BBa_J176124
type: ComponentDefinition
persistent identity: BBa J176124
display id: BBa_J176124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcDNA3.1+ zeo
creator: Karmella Haynes, Andrew Younger
created: 2011-10-21T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pcDNA3.1+ zeo/ ΔpCMV, based on pcDNA3.1+; no promoter; amp, zeo
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: SV40
sequence annotation: EM7
sequence annotation: Ampicillin resistance
sequence annotation: bla
sequence annotation: Zeocin resistance
sequence annotation: CMV promoter remnant
sequence annotation: DD122B
sequence annotation: BGH
sequence annotation: f1 origin
sequence annotation: pUC origin
sequence annotation: SpeI EcoRI PstI NotI XbaI
sequence: https://synbiohub.org/public/igem/BBa_J176124_sequence/1

SV40
type: SequenceAnnotation
persistent identity: annotation2167188
display id: annotation2167188
version: 1
top level: BBa_J176124
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167188/range2167188/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167188/range2167188/1
type: Range
persistent identity: range2167188
display id: range2167188
version: 1
direction: direction/forward
top level: BBa_J176124
start: 2270
end: 2400
orientation: inline

EM7
type: SequenceAnnotation
persistent identity: annotation2167185
display id: annotation2167185
version: 1
top level: BBa_J176124
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167185/range2167185/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167185/range2167185/1
type: Range
persistent identity: range2167185
display id: range2167185
version: 1
direction: direction/forward
top level: BBa_J176124
start: 1548
end: 1614
orientation: inline

Ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation2167190
display id: annotation2167190
version: 1
top level: BBa_J176124
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167190/range2167190/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167190/range2167190/1
type: Range
persistent identity: range2167190
display id: range2167190
version: 1
direction: direction/reverse
top level: BBa_J176124
start: 3598
end: 4458
orientation: inline

SV40
type: SequenceAnnotation
persistent identity: annotation2167184
display id: annotation2167184
version: 1
top level: BBa_J176124
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167184/range2167184/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167184/range2167184/1
type: Range
persistent identity: range2167184
display id: range2167184
version: 1
direction: direction/forward
top level: BBa_J176124
start: 1045
end: 1388
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2167191
display id: annotation2167191
version: 1
top level: BBa_J176124
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167191/range2167191/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167191/range2167191/1
type: Range
persistent identity: range2167191
display id: range2167191
version: 1
direction: direction/reverse
top level: BBa_J176124
start: 4459
end: 4557
orientation: inline

Zeocin resistance
type: SequenceAnnotation
persistent identity: annotation2167186
display id: annotation2167186
version: 1
top level: BBa_J176124
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167186/range2167186/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167186/range2167186/1
type: Range
persistent identity: range2167186
display id: range2167186
version: 1
direction: direction/forward
top level: BBa_J176124
start: 1615
end: 1989
orientation: inline

CMV promoter remnant
type: SequenceAnnotation
persistent identity: annotation2167179
display id: annotation2167179
version: 1
top level: BBa_J176124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167179/range2167179/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167179/range2167179/1
type: Range
persistent identity: range2167179
display id: range2167179
version: 1
direction: direction/forward
top level: BBa_J176124
start: 232
end: 249
orientation: inline

SV40
type: SequenceAnnotation
persistent identity: annotation2167187
display id: annotation2167187
version: 1
top level: BBa_J176124
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167187/range2167187/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167187/range2167187/1
type: Range
persistent identity: range2167187
display id: range2167187
version: 1
direction: direction/forward
top level: BBa_J176124
start: 2003
end: 2084
orientation: inline

DD122B
type: SequenceAnnotation
persistent identity: annotation2167205
display id: annotation2167205
version: 1
top level: BBa_J176124
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167205/range2167205/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167205/range2167205/1
type: Range
persistent identity: range2167205
display id: range2167205
version: 1
direction: direction/forward
top level: BBa_J176124
start: 186
end: 203
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2167181
display id: annotation2167181
version: 1
top level: BBa_J176124
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167181/range2167181/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167181/range2167181/1
type: Range
persistent identity: range2167181
display id: range2167181
version: 1
direction: direction/reverse
top level: BBa_J176124
start: 336
end: 353
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2167182
display id: annotation2167182
version: 1
top level: BBa_J176124
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167182/range2167182/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167182/range2167182/1
type: Range
persistent identity: range2167182
display id: range2167182
version: 1
direction: direction/forward
top level: BBa_J176124
start: 342
end: 566
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2167183
display id: annotation2167183
version: 1
top level: BBa_J176124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167183/range2167183/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167183/range2167183/1
type: Range
persistent identity: range2167183
display id: range2167183
version: 1
direction: direction/forward
top level: BBa_J176124
start: 612
end: 1040
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2167189
display id: annotation2167189
version: 1
top level: BBa_J176124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167189/range2167189/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167189/range2167189/1
type: Range
persistent identity: range2167189
display id: range2167189
version: 1
direction: direction/reverse
top level: BBa_J176124
start: 2783
end: 3453
orientation: inline

SpeI EcoRI PstI NotI XbaI
type: SequenceAnnotation
persistent identity: annotation2167180
display id: annotation2167180
version: 1
top level: BBa_J176124
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J176124/annotation2167180/range2167180/1

https://synbiohub.org/public/igem/BBa_J176124/annotation2167180/range2167180/1
type: Range
persistent identity: range2167180
display id: range2167180
version: 1
direction: direction/forward
top level: BBa_J176124
start: 250
end: 324
orientation: inline

BBa_K187000
type: ComponentDefinition
persistent identity: BBa K187000
display id: BBa_K187000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAB Universal Plasmid for BioBytes Gene Assembly System
creator: Team BioBytes, University of Alberta
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _286_
m user id: 0
owner id: 4048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The boundary sequences required for sticky end production for the BioBytes gene assembly system are acquired by inserting gene sequences destined to become bytes into either of the host plasmids pAB or pBA. Both are derivatives of pUC19 with the following key modifications: 1. Replacement of the pUC19 MCS with the MCS shown in figure 1. 2. Elimination of the Z alpha fragment 3. Elimination of the restriction sites Bts1 and BspQ1 Functional features include: 1. The ability to adapt biobricks to the Byte format using the Xba1 and Pst1 sites contained in the MCS. 2. The ability to adapt the in the ASKA collection (Kitagawa 2005) to the Byte format (4000+ E. coli K12 ORFs). ORFs are cloned into an expression vector, accompanied by a 6xHis tag at the N-terminus and a GFP fusion on the C-terminus. ASKA parts can be freed by digestion with SfiI, leaving noncomplementary 3 bp 3' sticky ends, which can be cloned directionally into the MCS after digestion with BstAPI. Since the ASKA parts are freed from the interior of the CDS, the MCS provides a start codon 10 bp downstream of the RBS, and a stop codon (TGA) downstream of the insertion. ORFs cloned from ASKA plasmids replace the N-terminal methionine of the predicted ORF with Met-Ala-Leu-Arg-Ala, and append Cys-Leu at the C-terminus. 3. Other potentially useful restriction sites have been included in the MCS as well as an RBS consensus positioned 8 bp upstream of the ATG used for ORF???s derived from 1. And 2 4. The ability to select for insert-containing constructs through the linearization of parental contaminants by cleavage with the rare- cutters SwaI and PmeI contained in the MCS. 5. Two routes for the production of 12 base overhangs by PCR using universal primers: A) PCR followed by nicking using the engineered enzymes Nb.BbvCI and Nb.BtsI. B) PCR followed by removal of a primer-encoded uracil residue using BioLab???s UserTM system. Primer positions and sequences are indicated for both.
mutable notes: Using these plasmids as well as universal primer sequences for the PCR extraction of bricks from the plasmids anyone will be able to use this system for their own rapid assembly. The design ensures that the existing catalog of BioBrick parts is compatible with the system, and can be easily cloned into the universal plasmids for brick generation. We believe that this new system is ready for both use and formalization, and we have prepared a BBF RFC detailing the use of BioBytes.
mutable provenance: Derivative of pUC19
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RBS
sequence annotation: XbaI
sequence annotation: amp resistance
sequence annotation: oriT
sequence annotation: B overhang
sequence annotation: A overhang
sequence annotation: AB cassette
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_K187000_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2061895
display id: annotation2061895
version: 1
top level: BBa_K187000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K187000/annotation2061895/range2061895/1

https://synbiohub.org/public/igem/BBa_K187000/annotation2061895/range2061895/1
type: Range
persistent identity: range2061895
display id: range2061895
version: 1
direction: direction/reverse
top level: BBa_K187000
start: 785
end: 792
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2042848
display id: annotation2042848
version: 1
top level: BBa_K187000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187000/annotation2042848/range2042848/1

https://synbiohub.org/public/igem/BBa_K187000/annotation2042848/range2042848/1
type: Range
persistent identity: range2042848
display id: range2042848
version: 1
direction: direction/forward
top level: BBa_K187000
start: 793
end: 798
orientation: inline

amp resistance
type: SequenceAnnotation
persistent identity: annotation2042843
display id: annotation2042843
version: 1
top level: BBa_K187000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187000/annotation2042843/range2042843/1

https://synbiohub.org/public/igem/BBa_K187000/annotation2042843/range2042843/1
type: Range
persistent identity: range2042843
display id: range2042843
version: 1
direction: direction/forward
top level: BBa_K187000
start: 1013
end: 1873
orientation: inline

oriT
type: SequenceAnnotation
persistent identity: annotation2042847
display id: annotation2042847
version: 1
top level: BBa_K187000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187000/annotation2042847/range2042847/1

https://synbiohub.org/public/igem/BBa_K187000/annotation2042847/range2042847/1
type: Range
persistent identity: range2042847
display id: range2042847
version: 1
direction: direction/reverse
top level: BBa_K187000
start: 29
end: 649
orientation: inline

B overhang
type: SequenceAnnotation
persistent identity: annotation2042845
display id: annotation2042845
version: 1
top level: BBa_K187000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K187000/annotation2042845/range2042845/1

https://synbiohub.org/public/igem/BBa_K187000/annotation2042845/range2042845/1
type: Range
persistent identity: range2042845
display id: range2042845
version: 1
direction: direction/reverse
top level: BBa_K187000
start: 846
end: 857
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation2042846
display id: annotation2042846
version: 1
top level: BBa_K187000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K187000/annotation2042846/range2042846/1

https://synbiohub.org/public/igem/BBa_K187000/annotation2042846/range2042846/1
type: Range
persistent identity: range2042846
display id: range2042846
version: 1
direction: direction/forward
top level: BBa_K187000
start: 772
end: 783
orientation: inline

AB cassette
type: SequenceAnnotation
persistent identity: annotation2042844
display id: annotation2042844
version: 1
top level: BBa_K187000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187000/annotation2042844/range2042844/1

https://synbiohub.org/public/igem/BBa_K187000/annotation2042844/range2042844/1
type: Range
persistent identity: range2042844
display id: range2042844
version: 1
direction: direction/forward
top level: BBa_K187000
start: 751
end: 882
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2042849
display id: annotation2042849
version: 1
top level: BBa_K187000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187000/annotation2042849/range2042849/1

https://synbiohub.org/public/igem/BBa_K187000/annotation2042849/range2042849/1
type: Range
persistent identity: range2042849
display id: range2042849
version: 1
direction: direction/forward
top level: BBa_K187000
start: 838
end: 843
orientation: inline

pSB3K1
type: ComponentDefinition
persistent identity: pSB3K1
display id: pSB3K1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pACYC177 converted into BioBrick vector (formerly BBa_I739202)
creator: Stefan Luzi
created: 2007-10-22T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: partial Tn903 repressor fragment
sequence annotation: BioBrick suffix
sequence annotation: p15A replication origin
sequence annotation: Tn903 inverted repeat
sequence annotation: Tn3 inverted repeat
sequence annotation: partial ampicillin resistance marker (nonfunctional)
sequence annotation: kanamycin resistance marker
sequence annotation: BioBrick prefix
sequence: https://synbiohub.org/public/igem/pSB3K1_sequence/1

partial Tn903 repressor fragment
type: SequenceAnnotation
persistent identity: annotation1969628
display id: annotation1969628
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969628/range1969628/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969628/range1969628/1
type: Range
persistent identity: range1969628
display id: range1969628
version: 1
direction: direction/forward
top level: pSB3K1
start: 2742
end: 3036
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969629
display id: annotation1969629
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969629/range1969629/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969629/range1969629/1
type: Range
persistent identity: range1969629
display id: range1969629
version: 1
direction: direction/forward
top level: pSB3K1
start: 1
end: 21
orientation: inline

p15A replication origin
type: SequenceAnnotation
persistent identity: annotation1969622
display id: annotation1969622
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969622/range1969622/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969622/range1969622/1
type: Range
persistent identity: range1969622
display id: range1969622
version: 1
direction: direction/forward
top level: pSB3K1
start: 483
end: 1312
orientation: inline

Tn903 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969627
display id: annotation1969627
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969627/range1969627/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969627/range1969627/1
type: Range
persistent identity: range1969627
display id: range1969627
version: 1
direction: direction/forward
top level: pSB3K1
start: 2515
end: 2742
orientation: inline

Tn3 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969625
display id: annotation1969625
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969625/range1969625/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969625/range1969625/1
type: Range
persistent identity: range1969625
display id: range1969625
version: 1
direction: direction/forward
top level: pSB3K1
start: 445
end: 482
orientation: inline

partial ampicillin resistance marker (nonfunctional)
type: SequenceAnnotation
persistent identity: annotation1969624
display id: annotation1969624
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969624/range1969624/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969624/range1969624/1
type: Range
persistent identity: range1969624
display id: range1969624
version: 1
direction: direction/forward
top level: pSB3K1
start: 22
end: 332
orientation: inline

kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation1969623
display id: annotation1969623
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969623/range1969623/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969623/range1969623/1
type: Range
persistent identity: range1969623
display id: range1969623
version: 1
direction: direction/forward
top level: pSB3K1
start: 1637
end: 2453
orientation: inline

Tn903 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969626
display id: annotation1969626
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969626/range1969626/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969626/range1969626/1
type: Range
persistent identity: range1969626
display id: range1969626
version: 1
direction: direction/forward
top level: pSB3K1
start: 1307
end: 1534
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969630
display id: annotation1969630
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969630/range1969630/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969630/range1969630/1
type: Range
persistent identity: range1969630
display id: range1969630
version: 1
direction: direction/forward
top level: pSB3K1
start: 3043
end: 3064
orientation: inline

BBa_K1223009
type: ComponentDefinition
persistent identity: BBa K1223009
display id: BBa_K1223009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pUC57 backbone (REVERSED)
creator: Orr Schlesinger
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:09:43Z
discontinued: false
dominant: false
group u list: _1537_
m user id: 0
owner id: 18143
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pUC57 backbone - digested between BamHI and XbaI. (reverse-complement sequence)
mutable notes: cloning of P.A.S.E. 1&2 cassettes.
mutable provenance: genebank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: b-lactamase CDS (ampR)
sequence annotation: Rep. origin
sequence: https://synbiohub.org/public/igem/BBa_K1223009_sequence/1

b-lactamase CDS (ampR)
type: SequenceAnnotation
persistent identity: annotation2336786
display id: annotation2336786
version: 1
top level: BBa_K1223009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223009/annotation2336786/range2336786/1

https://synbiohub.org/public/igem/BBa_K1223009/annotation2336786/range2336786/1
type: Range
persistent identity: range2336786
display id: range2336786
version: 1
direction: direction/forward
top level: BBa_K1223009
start: 626
end: 1486
orientation: inline

Rep. origin
type: SequenceAnnotation
persistent identity: annotation2336789
display id: annotation2336789
version: 1
top level: BBa_K1223009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223009/annotation2336789/range2336789/1

https://synbiohub.org/public/igem/BBa_K1223009/annotation2336789/range2336789/1
type: Range
persistent identity: range2336789
display id: range2336789
version: 1
direction: direction/forward
top level: BBa_K1223009
start: 2246
end: 2247
orientation: inline

BBa_K1444028
type: ComponentDefinition
persistent identity: BBa K1444028
display id: BBa_K1444028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pET28b+ cloning vector from BioBasic
creator: Dave Curran
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
group u list: _1822_
m user id: 0
owner id: 1972
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Desc
mutable notes: design
mutable provenance: source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: M13 replication origin
sequence annotation: Cloning site
sequence annotation: ColE1 replication origin
sequence annotation: F1 replication origin
sequence annotation: kanR
sequence: https://synbiohub.org/public/igem/BBa_K1444028_sequence/1

M13 replication origin
type: SequenceAnnotation
persistent identity: annotation2427447
display id: annotation2427447
version: 1
top level: BBa_K1444028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1444028/annotation2427447/range2427447/1

https://synbiohub.org/public/igem/BBa_K1444028/annotation2427447/range2427447/1
type: Range
persistent identity: range2427447
display id: range2427447
version: 1
direction: direction/reverse
top level: BBa_K1444028
start: 4902
end: 5357
orientation: inline

Cloning site
type: SequenceAnnotation
persistent identity: annotation2427443
display id: annotation2427443
version: 1
top level: BBa_K1444028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1444028/annotation2427443/range2427443/1

https://synbiohub.org/public/igem/BBa_K1444028/annotation2427443/range2427443/1
type: Range
persistent identity: range2427443
display id: range2427443
version: 1
direction: direction/forward
top level: BBa_K1444028
start: 192
end: 198
orientation: inline

ColE1 replication origin
type: SequenceAnnotation
persistent identity: annotation2427444
display id: annotation2427444
version: 1
top level: BBa_K1444028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1444028/annotation2427444/range2427444/1

https://synbiohub.org/public/igem/BBa_K1444028/annotation2427444/range2427444/1
type: Range
persistent identity: range2427444
display id: range2427444
version: 1
direction: direction/reverse
top level: BBa_K1444028
start: 3266
end: 3948
orientation: inline

F1 replication origin
type: SequenceAnnotation
persistent identity: annotation2427448
display id: annotation2427448
version: 1
top level: BBa_K1444028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1444028/annotation2427448/range2427448/1

https://synbiohub.org/public/igem/BBa_K1444028/annotation2427448/range2427448/1
type: Range
persistent identity: range2427448
display id: range2427448
version: 1
direction: direction/forward
top level: BBa_K1444028
start: 5034
end: 5340
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation2427445
display id: annotation2427445
version: 1
top level: BBa_K1444028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1444028/annotation2427445/range2427445/1

https://synbiohub.org/public/igem/BBa_K1444028/annotation2427445/range2427445/1
type: Range
persistent identity: range2427445
display id: range2427445
version: 1
direction: direction/forward
top level: BBa_K1444028
start: 3994
end: 4806
orientation: inline

BBa_K864005
type: ComponentDefinition
persistent identity: BBa K864005
display id: BBa_K864005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. The resistance cassette is flanked by FRT sites (BBa_J61020) which allows for subsequent removal of the resistance cassette from the chromosome by the FLP recombinase. The pSB8x15 series are especially suitable for carrying the FLP recombinase, since they can be removed by incubation at 42??C.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Lux terminator
sequence annotation: Lambda Red forward primer
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: rrnC terminator
sequence annotation: VR
sequence annotation: Lambda Red reverse primer
sequence annotation: TonB terminator
sequence: https://synbiohub.org/public/igem/BBa_K864005_sequence/1

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2200051
display id: annotation2200051
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200051/range2200051/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200051/range2200051/1
type: Range
persistent identity: range2200051
display id: range2200051
version: 1
direction: direction/forward
top level: BBa_K864005
start: 2454
end: 2499
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2200050
display id: annotation2200050
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200050/range2200050/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200050/range2200050/1
type: Range
persistent identity: range2200050
display id: range2200050
version: 1
direction: direction/forward
top level: BBa_K864005
start: 2433
end: 2450
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2200048
display id: annotation2200048
version: 1
top level: BBa_K864005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200048/range2200048/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200048/range2200048/1
type: Range
persistent identity: range2200048
display id: range2200048
version: 1
direction: direction/forward
top level: BBa_K864005
start: 190
end: 2405
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2200047
display id: annotation2200047
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200047/range2200047/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200047/range2200047/1
type: Range
persistent identity: range2200047
display id: range2200047
version: 1
direction: direction/forward
top level: BBa_K864005
start: 136
end: 175
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2200046
display id: annotation2200046
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200046/range2200046/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200046/range2200046/1
type: Range
persistent identity: range2200046
display id: range2200046
version: 1
direction: direction/reverse
top level: BBa_K864005
start: 116
end: 135
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2200041
display id: annotation2200041
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200041/range2200041/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200041/range2200041/1
type: Range
persistent identity: range2200041
display id: range2200041
version: 1
direction: direction/reverse
top level: BBa_K864005
start: 18
end: 38
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2200045
display id: annotation2200045
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200045/range2200045/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200045/range2200045/1
type: Range
persistent identity: range2200045
display id: range2200045
version: 1
direction: direction/forward
top level: BBa_K864005
start: 47
end: 115
orientation: inline

pSB8S15
type: ComponentDefinition
persistent identity: BBa K864019
display id: BBa_K864019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8S15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864019_sequence/1

BBa_K390100
type: ComponentDefinition
persistent identity: BBa K390100
display id: BBa_K390100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A3_IntK - General Integration Plasmid with KmR
creator: Cody Tramp
created: 2010-08-02T11:00:00Z
modified: 2015-05-08T01:12:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _518_
m user id: 0
owner id: 6788
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: (coming soon)
mutable notes: (coming soon)
mutable provenance: (coming soon)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

BBa_K2151666
type: ComponentDefinition
persistent identity: BBa K2151666
display id: BBa_K2151666
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMG36ET
creator: Matej Vucak, Katy Baker, James Provan
created: 2016-08-29T11:00:00Z
modified: 2016-10-09T06:24:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2621_
m user id: 32487
owner id: 27285
owning group id: 9
status: status/Planning
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K2151666_sequence/1

BBa_K1445002
type: ComponentDefinition
persistent identity: BBa K1445002
display id: BBa_K1445002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBricked M13 phagemid vector
creator: Josephina Hendrix
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1823_
m user id: 0
owner id: 22016
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This biobrick backbone has the high copy number pUC19 origin of replication and an Ampicillin resistance marker. Part also contains the M13 origin of replication which allows the phagemid to be taken up by the M13 phage. Vector does not contain any expressible phage genes; therefore, a Helper Phagemid is required for phage production. Vector contains the following: iGEM VF2 and VR sequencing sites T7 promoters that flank the MCS Translational stop sequences (BBa_B0042) that flank the MCS Transcriptional terminator BBa_B0055 upstream of the MCS Transcriptional terminator BBa_B0054 downstream of the MCS
mutable notes: Plasmid was designed to have the iGEM VF2 and VR sequencing sites along with translational and transcriptional terminators flanking the MCS.
mutable provenance: Limtus28i phagemid vector from NEB. Areas flanking MCS were cloned from pSB3C5 backbone.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1445002_sequence/1

BBa_K305011
type: ComponentDefinition
persistent identity: BBa K305011
display id: BBa_K305011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Subtilin inducible expression plasmid for Bacillus subtilis
creator: Maarten van den Nieuwenhof, Ramon Sieber, Arend Jan Suk
created: 2010-10-18T11:00:00Z
modified: 2015-05-08T01:11:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _426_
m user id: 0
owner id: 5922
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Discription will follow...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: repC
sequence annotation: spaS
sequence annotation: Terminator
sequence annotation: BioBrick prefix
sequence annotation: BioBrick suffix
sequence annotation: repA
sequence annotation: BamHI site for circularization
sequence annotation: Brick sequencing primer (3BR) binding site
sequence annotation: Chloramphenicol resistance
sequence annotation: Brick sequencing primer (3CF) binding site
sequence: https://synbiohub.org/public/igem/BBa_K305011_sequence/1

repC
type: SequenceAnnotation
persistent identity: annotation2092362
display id: annotation2092362
version: 1
top level: BBa_K305011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K305011/annotation2092362/range2092362/1

https://synbiohub.org/public/igem/BBa_K305011/annotation2092362/range2092362/1
type: Range
persistent identity: range2092362
display id: range2092362
version: 1
direction: direction/forward
top level: BBa_K305011
start: 681
end: 890
orientation: inline

spaS
type: SequenceAnnotation
persistent identity: annotation2092363
display id: annotation2092363
version: 1
top level: BBa_K305011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K305011/annotation2092363/range2092363/1

https://synbiohub.org/public/igem/BBa_K305011/annotation2092363/range2092363/1
type: Range
persistent identity: range2092363
display id: range2092363
version: 1
direction: direction/forward
top level: BBa_K305011
start: 3061
end: 3138
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2092365
display id: annotation2092365
version: 1
top level: BBa_K305011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K305011/annotation2092365/range2092365/1

https://synbiohub.org/public/igem/BBa_K305011/annotation2092365/range2092365/1
type: Range
persistent identity: range2092365
display id: range2092365
version: 1
direction: direction/forward
top level: BBa_K305011
start: 103
end: 155
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2092359
display id: annotation2092359
version: 1
top level: BBa_K305011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K305011/annotation2092359/range2092359/1

https://synbiohub.org/public/igem/BBa_K305011/annotation2092359/range2092359/1
type: Range
persistent identity: range2092359
display id: range2092359
version: 1
direction: direction/forward
top level: BBa_K305011
start: 3141
end: 3162
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2092344
display id: annotation2092344
version: 1
top level: BBa_K305011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K305011/annotation2092344/range2092344/1

https://synbiohub.org/public/igem/BBa_K305011/annotation2092344/range2092344/1
type: Range
persistent identity: range2092344
display id: range2092344
version: 1
direction: direction/forward
top level: BBa_K305011
start: 1
end: 21
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2092360
display id: annotation2092360
version: 1
top level: BBa_K305011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K305011/annotation2092360/range2092360/1

https://synbiohub.org/public/igem/BBa_K305011/annotation2092360/range2092360/1
type: Range
persistent identity: range2092360
display id: range2092360
version: 1
direction: direction/forward
top level: BBa_K305011
start: 1158
end: 1856
orientation: inline

BamHI site for circularization
type: SequenceAnnotation
persistent identity: annotation2098404
display id: annotation2098404
version: 1
top level: BBa_K305011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K305011/annotation2098404/range2098404/1

https://synbiohub.org/public/igem/BBa_K305011/annotation2098404/range2098404/1
type: Range
persistent identity: range2098404
display id: range2098404
version: 1
direction: direction/forward
top level: BBa_K305011
start: 3163
end: 3168
orientation: inline

Brick sequencing primer (3BR) binding site
type: SequenceAnnotation
persistent identity: annotation2092372
display id: annotation2092372
version: 1
top level: BBa_K305011
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K305011/annotation2092372/range2092372/1

https://synbiohub.org/public/igem/BBa_K305011/annotation2092372/range2092372/1
type: Range
persistent identity: range2092372
display id: range2092372
version: 1
direction: direction/reverse
top level: BBa_K305011
start: 60
end: 78
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2092361
display id: annotation2092361
version: 1
top level: BBa_K305011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K305011/annotation2092361/range2092361/1

https://synbiohub.org/public/igem/BBa_K305011/annotation2092361/range2092361/1
type: Range
persistent identity: range2092361
display id: range2092361
version: 1
direction: direction/forward
top level: BBa_K305011
start: 2315
end: 2965
orientation: inline

Brick sequencing primer (3CF) binding site
type: SequenceAnnotation
persistent identity: annotation2092371
display id: annotation2092371
version: 1
top level: BBa_K305011
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K305011/annotation2092371/range2092371/1

https://synbiohub.org/public/igem/BBa_K305011/annotation2092371/range2092371/1
type: Range
persistent identity: range2092371
display id: range2092371
version: 1
direction: direction/forward
top level: BBa_K305011
start: 2925
end: 2944
orientation: inline

BBa_I732952
type: ComponentDefinition
persistent identity: BBa I732952
display id: BBa_I732952
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptemplate1 + RBS.ARL2A0103
creator: Zhan Jian
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: {| border="1" |- |align="center"|'''Genotype''' |align="center"|'''Character''' |align="center"|'''Name''' |- |Top10/pT-TERM |So not express any repressors |align="center"|CR00 |- |Top10/pT-ARL4A0604 |Constitutively express ARL4A0604 |align="center"|CR01 |- |Top10/pT-ARL2A0203 |Constitutively express ARL2A0203 |align="center"|CR10 |- |Top10/pTet-ARL4A0604-ARL2A203 |Constitutively express ARL4A0604 and ARL2A0203 |align="center"|CR11 |}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: End of terminator
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: E. coli his operon terminator
sequence annotation: Stem loop
sequence annotation: XbaI
sequence annotation: Deletion of AC
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence annotation: BioBrick suffix
sequence annotation: Ptemplate1?
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: EcoRI
sequence annotation: pBR322 origin
sequence annotation: beta-lactamase (ampR) promoter
sequence: https://synbiohub.org/public/igem/BBa_I732952_sequence/1

End of terminator
type: SequenceAnnotation
persistent identity: annotation1975793
display id: annotation1975793
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975793/range1975793/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975793/range1975793/1
type: Range
persistent identity: range1975793
display id: range1975793
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 2094
end: 2097
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975797
display id: annotation1975797
version: 1
top level: BBa_I732952
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975797/range1975797/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975797/range1975797/1
type: Range
persistent identity: range1975797
display id: range1975797
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 157
end: 176
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1975794
display id: annotation1975794
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975794/range1975794/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975794/range1975794/1
type: Range
persistent identity: range1975794
display id: range1975794
version: 1
direction: direction/forward
top level: BBa_I732952
start: 22
end: 93
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975792
display id: annotation1975792
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975792/range1975792/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975792/range1975792/1
type: Range
persistent identity: range1975792
display id: range1975792
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 2107
end: 2126
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975790
display id: annotation1975790
version: 1
top level: BBa_I732952
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975790/range1975790/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975790/range1975790/1
type: Range
persistent identity: range1975790
display id: range1975790
version: 1
direction: direction/forward
top level: BBa_I732952
start: 2301
end: 2306
orientation: inline

Deletion of AC
type: SequenceAnnotation
persistent identity: annotation1975796
display id: annotation1975796
version: 1
top level: BBa_I732952
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975796/range1975796/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975796/range1975796/1
type: Range
persistent identity: range1975796
display id: range1975796
version: 1
direction: direction/forward
top level: BBa_I732952
start: 34
end: 35
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1975786
display id: annotation1975786
version: 1
top level: BBa_I732952
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975786/range1975786/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975786/range1975786/1
type: Range
persistent identity: range1975786
display id: range1975786
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 1035
end: 1895
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975789
display id: annotation1975789
version: 1
top level: BBa_I732952
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975789/range1975789/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975789/range1975789/1
type: Range
persistent identity: range1975789
display id: range1975789
version: 1
direction: direction/forward
top level: BBa_I732952
start: 1
end: 21
orientation: inline

Ptemplate1?
type: SequenceAnnotation
persistent identity: annotation1975798
display id: annotation1975798
version: 1
top level: BBa_I732952
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975798/range1975798/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975798/range1975798/1
type: Range
persistent identity: range1975798
display id: range1975798
version: 1
direction: direction/forward
top level: BBa_I732952
start: 2142
end: 2300
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975791
display id: annotation1975791
version: 1
top level: BBa_I732952
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975791/range1975791/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975791/range1975791/1
type: Range
persistent identity: range1975791
display id: range1975791
version: 1
direction: direction/forward
top level: BBa_I732952
start: 2018
end: 2037
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975788
display id: annotation1975788
version: 1
top level: BBa_I732952
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975788/range1975788/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975788/range1975788/1
type: Range
persistent identity: range1975788
display id: range1975788
version: 1
direction: direction/forward
top level: BBa_I732952
start: 2136
end: 2141
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975785
display id: annotation1975785
version: 1
top level: BBa_I732952
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975785/range1975785/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975785/range1975785/1
type: Range
persistent identity: range1975785
display id: range1975785
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 261
end: 880
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975795
display id: annotation1975795
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975795/range1975795/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975795/range1975795/1
type: Range
persistent identity: range1975795
display id: range1975795
version: 1
direction: direction/forward
top level: BBa_I732952
start: 30
end: 64
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1975787
display id: annotation1975787
version: 1
top level: BBa_I732952
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975787/range1975787/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975787/range1975787/1
type: Range
persistent identity: range1975787
display id: range1975787
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 1937
end: 1965
orientation: inline

BBa_K625005
type: ComponentDefinition
persistent identity: BBa K625005
display id: BBa_K625005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB6A5
creator: Michael Eichenberger
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
group u list: _797_
m user id: 0
owner id: 10153
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: low to medium copy standard biobrick plasmid backbone
mutable notes: Find out minimal pBR322 origin
mutable provenance: psb4A5, but ORI exchanged with ORI of pSEVA 171
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00000
component: BBa_B0053
component: BBa_B0042
component: BBa_B0054
component: BBa_G0002
component: BBa_B0044
component: BBa_B0062
component: BBa_B0043
component: BBa_K625004
component: BBa_G00001
component: BBa_G0004
component: BBa_G0003
component: BBa_P1002
component: BBa_G00100
component: BBa_G00102
component: BBa_B0055
sequence annotation: BBa_G0002
sequence annotation: BBa_B0055
sequence annotation: BBa_B0044
sequence annotation: BBa_G00001
sequence annotation: BBa_G0003
sequence annotation: BBa_G0004
sequence annotation: BBa_G00100
sequence annotation: BBa_B0062
sequence annotation: BBa_K625004
sequence annotation: BBa_B0042
sequence annotation: BBa_B0043
sequence annotation: BBa_G00000
sequence annotation: BBa_B0053
sequence annotation: BBa_G00102
sequence annotation: BBa_B0054
sequence annotation: BBa_P1002
sequence: https://synbiohub.org/public/igem/BBa_K625005_sequence/1

BBa_G00000
type: Component
persistent identity: component2141885
display id: component2141885
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0053
type: Component
persistent identity: component2141863
display id: component2141863
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0042
type: Component
persistent identity: component2141875
display id: component2141875
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0054
type: Component
persistent identity: component2141839
display id: component2141839
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G0002
type: Component
persistent identity: component2141855
display id: component2141855
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0044
type: Component
persistent identity: component2141829
display id: component2141829
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2141843
display id: component2141843
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0043
type: Component
persistent identity: component2141880
display id: component2141880
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_K625004
type: Component
persistent identity: component2141854
display id: component2141854
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_K625004/1

BBa_G00001
type: Component
persistent identity: component2141824
display id: component2141824
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G0004
type: Component
persistent identity: component2141861
display id: component2141861
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G0003
type: Component
persistent identity: component2141844
display id: component2141844
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_P1002
type: Component
persistent identity: component2141860
display id: component2141860
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_G00100
type: Component
persistent identity: component2141865
display id: component2141865
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00102
type: Component
persistent identity: component2141841
display id: component2141841
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component2141836
display id: component2141836
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0055
type: Component
persistent identity: component2141868
display id: component2141868
version: 1
top level: BBa_K625005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2141855
display id: annotation2141855
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141855/range2141855/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K625005/annotation2141855/range2141855/1
type: Range
persistent identity: range2141855
display id: range2141855
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 1569
end: 1576
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2141868
display id: annotation2141868
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141868/range2141868/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K625005/annotation2141868/range2141868/1
type: Range
persistent identity: range2141868
display id: range2141868
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 2619
end: 2696
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2141829
display id: annotation2141829
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141829/range2141829/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K625005/annotation2141829/range2141829/1
type: Range
persistent identity: range2141829
display id: range2141829
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 22
end: 34
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2141824
display id: annotation2141824
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141824/range2141824/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K625005/annotation2141824/range2141824/1
type: Range
persistent identity: range2141824
display id: range2141824
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 1
end: 21
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2141844
display id: annotation2141844
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141844/range2141844/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K625005/annotation2141844/range2141844/1
type: Range
persistent identity: range2141844
display id: range2141844
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 177
end: 183
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2141861
display id: annotation2141861
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141861/range2141861/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K625005/annotation2141861/range2141861/1
type: Range
persistent identity: range2141861
display id: range2141861
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 2520
end: 2526
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2141865
display id: annotation2141865
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141865/range2141865/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K625005/annotation2141865/range2141865/1
type: Range
persistent identity: range2141865
display id: range2141865
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 2599
end: 2618
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2141843
display id: annotation2141843
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141843/range2141843/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K625005/annotation2141843/range2141843/1
type: Range
persistent identity: range2141843
display id: range2141843
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 136
end: 176
orientation: inline

BBa_K625004
type: SequenceAnnotation
persistent identity: annotation2141854
display id: annotation2141854
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141854/range2141854/1
component: BBa_K625004

https://synbiohub.org/public/igem/BBa_K625005/annotation2141854/range2141854/1
type: Range
persistent identity: range2141854
display id: range2141854
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 184
end: 1568
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2141875
display id: annotation2141875
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141875/range2141875/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K625005/annotation2141875/range2141875/1
type: Range
persistent identity: range2141875
display id: range2141875
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 2697
end: 2708
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2141880
display id: annotation2141880
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141880/range2141880/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K625005/annotation2141880/range2141880/1
type: Range
persistent identity: range2141880
display id: range2141880
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 2709
end: 2721
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2141885
display id: annotation2141885
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141885/range2141885/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K625005/annotation2141885/range2141885/1
type: Range
persistent identity: range2141885
display id: range2141885
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 2722
end: 2743
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2141863
display id: annotation2141863
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141863/range2141863/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K625005/annotation2141863/range2141863/1
type: Range
persistent identity: range2141863
display id: range2141863
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 2527
end: 2598
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2141841
display id: annotation2141841
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141841/range2141841/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K625005/annotation2141841/range2141841/1
type: Range
persistent identity: range2141841
display id: range2141841
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 116
end: 135
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2141839
display id: annotation2141839
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141839/range2141839/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K625005/annotation2141839/range2141839/1
type: Range
persistent identity: range2141839
display id: range2141839
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 47
end: 115
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2141860
display id: annotation2141860
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141860/range2141860/1
component: BBa_P1002

https://synbiohub.org/public/igem/BBa_K625005/annotation2141860/range2141860/1
type: Range
persistent identity: range2141860
display id: range2141860
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 1577
end: 2519
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2141836
display id: annotation2141836
version: 1
top level: BBa_K625005
location: https://synbiohub.org/public/igem/BBa_K625005/annotation2141836/range2141836/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K625005/annotation2141836/range2141836/1
type: Range
persistent identity: range2141836
display id: range2141836
version: 1
direction: direction/reverse
top level: BBa_K625005
start: 35
end: 46
orientation: inline

BBa_J179000
type: ComponentDefinition
persistent identity: BBa J179000
display id: BBa_J179000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBS1C: Bacillus subtilis vector, amyE integration, CAM-resistance
creator: Jara Radeck
created: 2013-12-03T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This vector integrates into the amyE-site of Bacillus subtilis via double recombination. The two recombination sites flank a standard BioBrick multiple cloning site (with RFP-cassette), as well as a chloramphenicol resistance cassette (5 ??g/ml). The backbone does not contain the restriction sites EcoRI, XbaI, SpeI, PstI, NgoMIV nor AgeI. The vector is replicative in Escherichia coli and provides resistance to ampicillin (100 ??g/ml). Before transformation into B. subtilis, this vector should be linearized with ScaI.
mutable notes: This is one of three empty vectors with compatible integration loci and resistance cassettes to allow combination of up to three constructs of choice in one single strain.
mutable provenance: pDG1731
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ori
sequence annotation: amyE-front
sequence annotation: cat
sequence annotation: bla
sequence annotation: amyE-back
sequence: https://synbiohub.org/public/igem/BBa_J179000_sequence/1

ori
type: SequenceAnnotation
persistent identity: annotation2371577
display id: annotation2371577
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371577/range2371577/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371577/range2371577/1
type: Range
persistent identity: range2371577
display id: range2371577
version: 1
direction: direction/forward
top level: BBa_J179000
start: 3587
end: 4201
orientation: inline

amyE-front
type: SequenceAnnotation
persistent identity: annotation2371576
display id: annotation2371576
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371576/range2371576/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371576/range2371576/1
type: Range
persistent identity: range2371576
display id: range2371576
version: 1
direction: direction/forward
top level: BBa_J179000
start: 5546
end: 6066
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2371573
display id: annotation2371573
version: 1
top level: BBa_J179000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371573/range2371573/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371573/range2371573/1
type: Range
persistent identity: range2371573
display id: range2371573
version: 1
direction: direction/forward
top level: BBa_J179000
start: 358
end: 1008
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371575
display id: annotation2371575
version: 1
top level: BBa_J179000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371575/range2371575/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371575/range2371575/1
type: Range
persistent identity: range2371575
display id: range2371575
version: 1
direction: direction/reverse
top level: BBa_J179000
start: 4361
end: 5221
orientation: inline

amyE-back
type: SequenceAnnotation
persistent identity: annotation2371574
display id: annotation2371574
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371574/range2371574/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371574/range2371574/1
type: Range
persistent identity: range2371574
display id: range2371574
version: 1
direction: direction/forward
top level: BBa_J179000
start: 1379
end: 2407
orientation: inline

BBa_J23006
type: ComponentDefinition
persistent identity: BBa J23006
display id: BBa_J23006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Part-making plasmid with Ptet-XbaI-[biobrick]-SpeI-PstI
creator: John Anderson
created: 2006-06-30T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Plasmid for constructing biobrick parts that provides a minimal set of functions to observe activity.
mutable notes: N/A
mutable provenance: pSB1A2-I13521 See KAD062806-Construction of J23006 for details
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BBa_B0015 terminator
sequence annotation: beta-lactamase promoter
sequence annotation: EcoRI
sequence annotation: pBR322 origin
sequence annotation: rrnB T1 terminator
sequence annotation: BioBrick suffix
sequence annotation: R0040 (pTet promoter)
sequence annotation: ColE1 origin
sequence annotation: XbaI
sequence annotation: NotI
sequence annotation: beta-lactamase ampicillin resistance marker
sequence: https://synbiohub.org/public/igem/BBa_J23006_sequence/1

BBa_B0015 terminator
type: SequenceAnnotation
persistent identity: annotation1975722
display id: annotation1975722
version: 1
top level: BBa_J23006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975722/range1975722/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975722/range1975722/1
type: Range
persistent identity: range1975722
display id: range1975722
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2158
end: 2286
orientation: inline

beta-lactamase promoter
type: SequenceAnnotation
persistent identity: annotation1975725
display id: annotation1975725
version: 1
top level: BBa_J23006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975725/range1975725/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975725/range1975725/1
type: Range
persistent identity: range1975725
display id: range1975725
version: 1
direction: direction/reverse
top level: BBa_J23006
start: 1937
end: 1965
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975728
display id: annotation1975728
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975728/range1975728/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975728/range1975728/1
type: Range
persistent identity: range1975728
display id: range1975728
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2136
end: 2141
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975726
display id: annotation1975726
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975726/range1975726/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975726/range1975726/1
type: Range
persistent identity: range1975726
display id: range1975726
version: 1
direction: direction/forward
top level: BBa_J23006
start: 261
end: 880
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975724
display id: annotation1975724
version: 1
top level: BBa_J23006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975724/range1975724/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975724/range1975724/1
type: Range
persistent identity: range1975724
display id: range1975724
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2169
end: 2212
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975727
display id: annotation1975727
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975727/range1975727/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975727/range1975727/1
type: Range
persistent identity: range1975727
display id: range1975727
version: 1
direction: direction/forward
top level: BBa_J23006
start: 1
end: 21
orientation: inline

R0040 (pTet promoter)
type: SequenceAnnotation
persistent identity: annotation1975720
display id: annotation1975720
version: 1
top level: BBa_J23006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975720/range1975720/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975720/range1975720/1
type: Range
persistent identity: range1975720
display id: range1975720
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2295
end: 2348
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1975721
display id: annotation1975721
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975721/range1975721/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975721/range1975721/1
type: Range
persistent identity: range1975721
display id: range1975721
version: 1
direction: direction/reverse
top level: BBa_J23006
start: 258
end: 940
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975730
display id: annotation1975730
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975730/range1975730/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975730/range1975730/1
type: Range
persistent identity: range1975730
display id: range1975730
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2350
end: 2355
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975729
display id: annotation1975729
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975729/range1975729/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975729/range1975729/1
type: Range
persistent identity: range1975729
display id: range1975729
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2142
end: 2149
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1975723
display id: annotation1975723
version: 1
top level: BBa_J23006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975723/range1975723/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975723/range1975723/1
type: Range
persistent identity: range1975723
display id: range1975723
version: 1
direction: direction/reverse
top level: BBa_J23006
start: 1035
end: 1895
orientation: inline

BBa_M36097
type: ComponentDefinition
persistent identity: BBa M36097
display id: BBa_M36097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mammalian sensor test rig
creator: Joseph Shih
created: 2013-04-22T11:00:00Z
modified: 2015-05-08T01:14:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 10436
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Mammalian test vector for sensors. Has a Comet protein coding sequence which produces a GFP-like protein. Comes with high copy number origin and ampicillin resistance; can replace with low copy number origin and kanamycin resistance if ordering from DNA 2.0.
mutable notes: Comet is a protein developed by DNA2.0
mutable provenance: All of the parts were designed by BIOE44 instructors in conjunction with DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: SV40 polyA
sequence annotation: student sensors go here
sequence annotation: pUCori
sequence annotation: kozak
sequence annotation: SV40 promoter
sequence annotation: rpo_bla transcription terminator
sequence annotation: BGH polyA
sequence annotation: AmpR
sequence annotation: puromycinR
sequence annotation: Pamp
sequence annotation: Comet
sequence: https://synbiohub.org/public/igem/BBa_M36097_sequence/1

SV40 polyA
type: SequenceAnnotation
persistent identity: annotation2215722
display id: annotation2215722
version: 1
top level: BBa_M36097
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_M36097/annotation2215722/range2215722/1

https://synbiohub.org/public/igem/BBa_M36097/annotation2215722/range2215722/1
type: Range
persistent identity: range2215722
display id: range2215722
version: 1
direction: direction/forward
top level: BBa_M36097
start: 2732
end: 2862
orientation: inline

student sensors go here
type: SequenceAnnotation
persistent identity: annotation2215716
display id: annotation2215716
version: 1
top level: BBa_M36097
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36097/annotation2215716/range2215716/1

https://synbiohub.org/public/igem/BBa_M36097/annotation2215716/range2215716/1
type: Range
persistent identity: range2215716
display id: range2215716
version: 1
direction: direction/forward
top level: BBa_M36097
start: 507
end: 576
orientation: inline

pUCori
type: SequenceAnnotation
persistent identity: annotation2215725
display id: annotation2215725
version: 1
top level: BBa_M36097
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36097/annotation2215725/range2215725/1

https://synbiohub.org/public/igem/BBa_M36097/annotation2215725/range2215725/1
type: Range
persistent identity: range2215725
display id: range2215725
version: 1
direction: direction/forward
top level: BBa_M36097
start: 3947
end: 4751
orientation: inline

kozak
type: SequenceAnnotation
persistent identity: annotation2215717
display id: annotation2215717
version: 1
top level: BBa_M36097
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_M36097/annotation2215717/range2215717/1

https://synbiohub.org/public/igem/BBa_M36097/annotation2215717/range2215717/1
type: Range
persistent identity: range2215717
display id: range2215717
version: 1
direction: direction/forward
top level: BBa_M36097
start: 587
end: 593
orientation: inline

SV40 promoter
type: SequenceAnnotation
persistent identity: annotation2215720
display id: annotation2215720
version: 1
top level: BBa_M36097
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_M36097/annotation2215720/range2215720/1

https://synbiohub.org/public/igem/BBa_M36097/annotation2215720/range2215720/1
type: Range
persistent identity: range2215720
display id: range2215720
version: 1
direction: direction/forward
top level: BBa_M36097
start: 1551
end: 1894
orientation: inline

rpo_bla transcription terminator
type: SequenceAnnotation
persistent identity: annotation2215715
display id: annotation2215715
version: 1
top level: BBa_M36097
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36097/annotation2215715/range2215715/1

https://synbiohub.org/public/igem/BBa_M36097/annotation2215715/range2215715/1
type: Range
persistent identity: range2215715
display id: range2215715
version: 1
direction: direction/forward
top level: BBa_M36097
start: 1
end: 425
orientation: inline

BGH polyA
type: SequenceAnnotation
persistent identity: annotation2215719
display id: annotation2215719
version: 1
top level: BBa_M36097
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_M36097/annotation2215719/range2215719/1

https://synbiohub.org/public/igem/BBa_M36097/annotation2215719/range2215719/1
type: Range
persistent identity: range2215719
display id: range2215719
version: 1
direction: direction/forward
top level: BBa_M36097
start: 1314
end: 1439
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2215723
display id: annotation2215723
version: 1
top level: BBa_M36097
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M36097/annotation2215723/range2215723/1

https://synbiohub.org/public/igem/BBa_M36097/annotation2215723/range2215723/1
type: Range
persistent identity: range2215723
display id: range2215723
version: 1
direction: direction/reverse
top level: BBa_M36097
start: 2908
end: 3768
orientation: inline

puromycinR
type: SequenceAnnotation
persistent identity: annotation2215721
display id: annotation2215721
version: 1
top level: BBa_M36097
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M36097/annotation2215721/range2215721/1

https://synbiohub.org/public/igem/BBa_M36097/annotation2215721/range2215721/1
type: Range
persistent identity: range2215721
display id: range2215721
version: 1
direction: direction/forward
top level: BBa_M36097
start: 2005
end: 2604
orientation: inline

Pamp
type: SequenceAnnotation
persistent identity: annotation2215724
display id: annotation2215724
version: 1
top level: BBa_M36097
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_M36097/annotation2215724/range2215724/1

https://synbiohub.org/public/igem/BBa_M36097/annotation2215724/range2215724/1
type: Range
persistent identity: range2215724
display id: range2215724
version: 1
direction: direction/reverse
top level: BBa_M36097
start: 3769
end: 3946
orientation: inline

Comet
type: SequenceAnnotation
persistent identity: annotation2215718
display id: annotation2215718
version: 1
top level: BBa_M36097
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M36097/annotation2215718/range2215718/1

https://synbiohub.org/public/igem/BBa_M36097/annotation2215718/range2215718/1
type: Range
persistent identity: range2215718
display id: range2215718
version: 1
direction: direction/forward
top level: BBa_M36097
start: 594
end: 1307
orientation: inline

BBa_K319043
type: ComponentDefinition
persistent identity: BBa K319043
display id: BBa_K319043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADE4 targeting vector
creator: Afnan Azizi
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _437_
m user id: 0
owner id: 7363
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a targeting vector for homologous recombination into the ADE4 locus of S. cerevisiae. This vector can be used in cloning free cloning (ref. 2010 uOttawa iGEM wiki). Yeast strains with an ADE1 or ADE2 deletion or mutation have red colonies; by integrating the constructs using our protocol the ADE4 gene is knocked out and the colonies express a white phenotype.
mutable notes: N/A
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Cm resistance
sequence annotation: Forward landing pad
sequence annotation: EcoRI
sequence annotation: PstI site
sequence annotation: PstI/NsiI mixed site
sequence annotation: ADE4 terminator
sequence annotation: EcoRI/MfeI mixed site
sequence annotation: G5C5G5 filler site
sequence annotation: Reverse landing pad
sequence annotation: ATM1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K319043_sequence/1

Cm resistance
type: SequenceAnnotation
persistent identity: annotation2109321
display id: annotation2109321
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109321/range2109321/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109321/range2109321/1
type: Range
persistent identity: range2109321
display id: range2109321
version: 1
direction: direction/reverse
top level: BBa_K319043
start: 1620
end: 2279
orientation: inline

Forward landing pad
type: SequenceAnnotation
persistent identity: annotation2109296
display id: annotation2109296
version: 1
top level: BBa_K319043
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109296/range2109296/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109296/range2109296/1
type: Range
persistent identity: range2109296
display id: range2109296
version: 1
direction: direction/forward
top level: BBa_K319043
start: 207
end: 226
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2109293
display id: annotation2109293
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109293/range2109293/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109293/range2109293/1
type: Range
persistent identity: range2109293
display id: range2109293
version: 1
direction: direction/forward
top level: BBa_K319043
start: 227
end: 232
orientation: inline

PstI site
type: SequenceAnnotation
persistent identity: annotation2109295
display id: annotation2109295
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109295/range2109295/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109295/range2109295/1
type: Range
persistent identity: range2109295
display id: range2109295
version: 1
direction: direction/forward
top level: BBa_K319043
start: 248
end: 253
orientation: inline

PstI/NsiI mixed site
type: SequenceAnnotation
persistent identity: annotation2109301
display id: annotation2109301
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109301/range2109301/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109301/range2109301/1
type: Range
persistent identity: range2109301
display id: range2109301
version: 1
direction: direction/forward
top level: BBa_K319043
start: 474
end: 479
orientation: inline

ADE4 terminator
type: SequenceAnnotation
persistent identity: annotation2109298
display id: annotation2109298
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109298/range2109298/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109298/range2109298/1
type: Range
persistent identity: range2109298
display id: range2109298
version: 1
direction: direction/forward
top level: BBa_K319043
start: 274
end: 473
orientation: inline

EcoRI/MfeI mixed site
type: SequenceAnnotation
persistent identity: annotation2109290
display id: annotation2109290
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109290/range2109290/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109290/range2109290/1
type: Range
persistent identity: range2109290
display id: range2109290
version: 1
direction: direction/forward
top level: BBa_K319043
start: 1
end: 6
orientation: inline

G5C5G5 filler site
type: SequenceAnnotation
persistent identity: annotation2109294
display id: annotation2109294
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109294/range2109294/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109294/range2109294/1
type: Range
persistent identity: range2109294
display id: range2109294
version: 1
direction: direction/forward
top level: BBa_K319043
start: 233
end: 247
orientation: inline

Reverse landing pad
type: SequenceAnnotation
persistent identity: annotation2109297
display id: annotation2109297
version: 1
top level: BBa_K319043
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109297/range2109297/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109297/range2109297/1
type: Range
persistent identity: range2109297
display id: range2109297
version: 1
direction: direction/reverse
top level: BBa_K319043
start: 254
end: 273
orientation: inline

ATM1 terminator
type: SequenceAnnotation
persistent identity: annotation2109292
display id: annotation2109292
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109292/range2109292/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109292/range2109292/1
type: Range
persistent identity: range2109292
display id: range2109292
version: 1
direction: direction/forward
top level: BBa_K319043
start: 7
end: 206
orientation: inline

BBa_J179002
type: ComponentDefinition
persistent identity: BBa J179002
display id: BBa_J179002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBs4S: Bacillus subtilis vector, thrC integration, spec resistance
creator: Jara Radeck
created: 2013-12-20T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Spectinomycin cassette. It has a ampicillin resistance for cloning in E.coli. This backbone is a BioBricked version of the B. subtilis vector pDG1731.
mutable notes: This vector is compatible with pBS1C and pBS2E in one strain.
mutable provenance: This BioBrick is part of the LMU 2012 igem project BacillusBioBrickBox For handling of B. subtilis vectors, please see here. The transformation into B. subtilis is explained here.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: hom, trC front
sequence annotation: spc
sequence annotation: bla
sequence annotation: thrC,thrB back
sequence annotation: ori
sequence: https://synbiohub.org/public/igem/BBa_J179002_sequence/1

hom, trC front
type: SequenceAnnotation
persistent identity: annotation2371770
display id: annotation2371770
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371770/range2371770/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371770/range2371770/1
type: Range
persistent identity: range2371770
display id: range2371770
version: 1
direction: direction/forward
top level: BBa_J179002
start: 3731
end: 4542
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2371766
display id: annotation2371766
version: 1
top level: BBa_J179002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371766/range2371766/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371766/range2371766/1
type: Range
persistent identity: range2371766
display id: range2371766
version: 1
direction: direction/forward
top level: BBa_J179002
start: 211
end: 885
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371769
display id: annotation2371769
version: 1
top level: BBa_J179002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371769/range2371769/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371769/range2371769/1
type: Range
persistent identity: range2371769
display id: range2371769
version: 1
direction: direction/reverse
top level: BBa_J179002
start: 2716
end: 3576
orientation: inline

thrC,thrB back
type: SequenceAnnotation
persistent identity: annotation2371767
display id: annotation2371767
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371767/range2371767/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371767/range2371767/1
type: Range
persistent identity: range2371767
display id: range2371767
version: 1
direction: direction/forward
top level: BBa_J179002
start: 1028
end: 1863
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2371768
display id: annotation2371768
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371768/range2371768/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371768/range2371768/1
type: Range
persistent identity: range2371768
display id: range2371768
version: 1
direction: direction/forward
top level: BBa_J179002
start: 1942
end: 2556
orientation: inline

BBa_J61002
type: ComponentDefinition
persistent identity: BBa J61002
display id: BBa_J61002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EX-Ptet-S-rbsRFP-P "RFP reporter"
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid for construction of promoter basic parts. Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly. Part J61002 is the tet promoter variant of the plasmid.
mutable notes: N/A
mutable provenance: PCR ca1020F/R on pSB1A2-I13521 (900bp, SpeI/PstI) Sub into pSB1A2-I13522 (SpeI/PstI) Product is pBca1020, will be an iGEM number ultimately ---- ca1020F Forward SpeI oligo for X-rbsRFP-P plasmid gcactACTAGTgaaagaggagaaatactagatggc ca1020R Reverse PstI oligo for X-rbsRFP-P plasmid ccggactgcagcggccgcttctagtatataaacg
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: PstI
sequence annotation: beta-lactamase (ampR, bla) promoter
sequence annotation: NotI
sequence annotation: mRFP
sequence annotation: ColE1 origin
sequence annotation: rrnB terminator
sequence annotation: pBR322 origin
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: BBa_B0015
sequence annotation: RBS
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: Ambiguity; should be a T
sequence annotation: SpeI
sequence annotation: BBa_B0034 RBS
sequence annotation: BioBrick prefix
sequence annotation: beta-lactamase ampicillin resistance marker
sequence: https://synbiohub.org/public/igem/BBa_J61002_sequence/1

PstI
type: SequenceAnnotation
persistent identity: annotation1975717
display id: annotation1975717
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975717/range1975717/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975717/range1975717/1
type: Range
persistent identity: range1975717
display id: range1975717
version: 1
direction: direction/forward
top level: BBa_J61002
start: 885
end: 890
orientation: inline

beta-lactamase (ampR, bla) promoter
type: SequenceAnnotation
persistent identity: annotation1892272
display id: annotation1892272
version: 1
top level: BBa_J61002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892272/range1892272/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892272/range1892272/1
type: Range
persistent identity: range1892272
display id: range1892272
version: 1
direction: direction/reverse
top level: BBa_J61002
start: 2728
end: 2756
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975718
display id: annotation1975718
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975718/range1975718/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975718/range1975718/1
type: Range
persistent identity: range1975718
display id: range1975718
version: 1
direction: direction/forward
top level: BBa_J61002
start: 878
end: 885
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation1892273
display id: annotation1892273
version: 1
top level: BBa_J61002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892273/range1892273/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892273/range1892273/1
type: Range
persistent identity: range1892273
display id: range1892273
version: 1
direction: direction/forward
top level: BBa_J61002
start: 27
end: 707
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1892275
display id: annotation1892275
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892275/range1892275/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892275/range1892275/1
type: Range
persistent identity: range1892275
display id: range1892275
version: 1
direction: direction/reverse
top level: BBa_J61002
start: 1049
end: 1731
orientation: inline

rrnB terminator
type: SequenceAnnotation
persistent identity: annotation1975713
display id: annotation1975713
version: 1
top level: BBa_J61002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975713/range1975713/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975713/range1975713/1
type: Range
persistent identity: range1975713
display id: range1975713
version: 1
direction: direction/forward
top level: BBa_J61002
start: 752
end: 795
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975714
display id: annotation1975714
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975714/range1975714/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975714/range1975714/1
type: Range
persistent identity: range1975714
display id: range1975714
version: 1
direction: direction/reverse
top level: BBa_J61002
start: 1052
end: 1671
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975768
display id: annotation1975768
version: 1
top level: BBa_J61002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975768/range1975768/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975768/range1975768/1
type: Range
persistent identity: range1975768
display id: range1975768
version: 1
direction: direction/reverse
top level: BBa_J61002
start: 948
end: 967
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation1892274
display id: annotation1892274
version: 1
top level: BBa_J61002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892274/range1892274/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892274/range1892274/1
type: Range
persistent identity: range1892274
display id: range1892274
version: 1
direction: direction/forward
top level: BBa_J61002
start: 741
end: 869
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975766
display id: annotation1975766
version: 1
top level: BBa_J61002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975766/range1975766/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975766/range1975766/1
type: Range
persistent identity: range1975766
display id: range1975766
version: 1
direction: direction/forward
top level: BBa_J61002
start: 9
end: 20
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975769
display id: annotation1975769
version: 1
top level: BBa_J61002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975769/range1975769/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975769/range1975769/1
type: Range
persistent identity: range1975769
display id: range1975769
version: 1
direction: direction/forward
top level: BBa_J61002
start: 2809
end: 2828
orientation: inline

Ambiguity; should be a T
type: SequenceAnnotation
persistent identity: annotation1975921
display id: annotation1975921
version: 1
top level: BBa_J61002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975921/range1975921/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975921/range1975921/1
type: Range
persistent identity: range1975921
display id: range1975921
version: 1
direction: direction/forward
top level: BBa_J61002
start: 2941
end: 2941
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1975716
display id: annotation1975716
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975716/range1975716/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975716/range1975716/1
type: Range
persistent identity: range1975716
display id: range1975716
version: 1
direction: direction/forward
top level: BBa_J61002
start: 2
end: 7
orientation: inline

BBa_B0034 RBS
type: SequenceAnnotation
persistent identity: annotation1975767
display id: annotation1975767
version: 1
top level: BBa_J61002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975767/range1975767/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975767/range1975767/1
type: Range
persistent identity: range1975767
display id: range1975767
version: 1
direction: direction/forward
top level: BBa_J61002
start: 9
end: 20
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1975715
display id: annotation1975715
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975715/range1975715/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975715/range1975715/1
type: Range
persistent identity: range1975715
display id: range1975715
version: 1
direction: direction/forward
top level: BBa_J61002
start: 2927
end: 2948
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1892276
display id: annotation1892276
version: 1
top level: BBa_J61002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892276/range1892276/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892276/range1892276/1
type: Range
persistent identity: range1892276
display id: range1892276
version: 1
direction: direction/reverse
top level: BBa_J61002
start: 1826
end: 2686
orientation: inline

BBa_K864003
type: ComponentDefinition
persistent identity: BBa K864003
display id: BBa_K864003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VF2
sequence annotation: Lambda Red forward primer
sequence annotation: T3 terminator
sequence annotation: P22 late terminator
sequence annotation: Promoter
sequence annotation: Lux terminator
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: Lambda Red reverse primer
sequence annotation: TonB terminator
sequence annotation: spcR
sequence annotation: VR
sequence annotation: rnC terminator
sequence: https://synbiohub.org/public/igem/BBa_K864003_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation2201244
display id: annotation2201244
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201244/range2201244/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201244/range2201244/1
type: Range
persistent identity: range2201244
display id: range2201244
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3649
end: 3668
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2201239
display id: annotation2201239
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201239/range2201239/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201239/range2201239/1
type: Range
persistent identity: range2201239
display id: range2201239
version: 1
direction: direction/forward
top level: BBa_K864003
start: 2433
end: 2450
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2201245
display id: annotation2201245
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201245/range2201245/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201245/range2201245/1
type: Range
persistent identity: range2201245
display id: range2201245
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3669
end: 3746
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2201243
display id: annotation2201243
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201243/range2201243/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201243/range2201243/1
type: Range
persistent identity: range2201243
display id: range2201243
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3608
end: 3648
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2201242
display id: annotation2201242
version: 1
top level: BBa_K864003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201242/range2201242/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201242/range2201242/1
type: Range
persistent identity: range2201242
display id: range2201242
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 3575
end: 3601
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2201240
display id: annotation2201240
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201240/range2201240/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201240/range2201240/1
type: Range
persistent identity: range2201240
display id: range2201240
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 2454
end: 2499
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2201238
display id: annotation2201238
version: 1
top level: BBa_K864003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201238/range2201238/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201238/range2201238/1
type: Range
persistent identity: range2201238
display id: range2201238
version: 1
direction: direction/forward
top level: BBa_K864003
start: 190
end: 2405
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2201234
display id: annotation2201234
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201234/range2201234/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201234/range2201234/1
type: Range
persistent identity: range2201234
display id: range2201234
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 18
end: 38
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2201235
display id: annotation2201235
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201235/range2201235/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201235/range2201235/1
type: Range
persistent identity: range2201235
display id: range2201235
version: 1
direction: direction/forward
top level: BBa_K864003
start: 47
end: 115
orientation: inline

spcR
type: SequenceAnnotation
persistent identity: annotation2201241
display id: annotation2201241
version: 1
top level: BBa_K864003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201241/range2201241/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201241/range2201241/1
type: Range
persistent identity: range2201241
display id: range2201241
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 2510
end: 3508
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2201236
display id: annotation2201236
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201236/range2201236/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201236/range2201236/1
type: Range
persistent identity: range2201236
display id: range2201236
version: 1
direction: direction/forward
top level: BBa_K864003
start: 116
end: 135
orientation: inline

rnC terminator
type: SequenceAnnotation
persistent identity: annotation2201237
display id: annotation2201237
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201237/range2201237/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201237/range2201237/1
type: Range
persistent identity: range2201237
display id: range2201237
version: 1
direction: direction/forward
top level: BBa_K864003
start: 136
end: 175
orientation: inline

BBa_M36099
type: ComponentDefinition
persistent identity: BBa M36099
display id: BBa_M36099
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. coli sensor test rig
creator: Joseph Shih
created: 2013-04-22T11:00:00Z
modified: 2015-05-08T01:14:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 10436
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: E. coli test vector for sensors. Has a Gemini protein coding sequence which produces a LacZalpha-GFP fusion protein. Comes with high copy number origin and ampicillin resistance; can replace with low copy number origin and kanamycin resistance if ordering from DNA 2.0.
mutable notes: None
mutable provenance: Gemini coding sequence sourced from Dr. Drew Endy and Martin et al., PlosOne 2009. The rest of the vector is sourced from DNA2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Pamp
sequence annotation: Comet GFP
sequence annotation: rrnB1T1T2 transcripton terminator
sequence annotation: student sensors go here
sequence annotation: pUCori
sequence annotation: RBS
sequence annotation: cloning cassette 1
sequence annotation: cloning cassette 2
sequence annotation: ampR
sequence annotation: T7 terminator
sequence annotation: rpo_bla transcription terminator
sequence: https://synbiohub.org/public/igem/BBa_M36099_sequence/1

Pamp
type: SequenceAnnotation
persistent identity: annotation2215547
display id: annotation2215547
version: 1
top level: BBa_M36099
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_M36099/annotation2215547/range2215547/1

https://synbiohub.org/public/igem/BBa_M36099/annotation2215547/range2215547/1
type: Range
persistent identity: range2215547
display id: range2215547
version: 1
direction: direction/forward
top level: BBa_M36099
start: 3461
end: 3638
orientation: inline

Comet GFP
type: SequenceAnnotation
persistent identity: annotation2215542
display id: annotation2215542
version: 1
top level: BBa_M36099
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M36099/annotation2215542/range2215542/1

https://synbiohub.org/public/igem/BBa_M36099/annotation2215542/range2215542/1
type: Range
persistent identity: range2215542
display id: range2215542
version: 1
direction: direction/forward
top level: BBa_M36099
start: 2449
end: 3162
orientation: inline

rrnB1T1T2 transcripton terminator
type: SequenceAnnotation
persistent identity: annotation2215544
display id: annotation2215544
version: 1
top level: BBa_M36099
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36099/annotation2215544/range2215544/1

https://synbiohub.org/public/igem/BBa_M36099/annotation2215544/range2215544/1
type: Range
persistent identity: range2215544
display id: range2215544
version: 1
direction: direction/forward
top level: BBa_M36099
start: 3270
end: 3460
orientation: inline

student sensors go here
type: SequenceAnnotation
persistent identity: annotation2215540
display id: annotation2215540
version: 1
top level: BBa_M36099
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36099/annotation2215540/range2215540/1

https://synbiohub.org/public/igem/BBa_M36099/annotation2215540/range2215540/1
type: Range
persistent identity: range2215540
display id: range2215540
version: 1
direction: direction/forward
top level: BBa_M36099
start: 2373
end: 2434
orientation: inline

pUCori
type: SequenceAnnotation
persistent identity: annotation2215538
display id: annotation2215538
version: 1
top level: BBa_M36099
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36099/annotation2215538/range2215538/1

https://synbiohub.org/public/igem/BBa_M36099/annotation2215538/range2215538/1
type: Range
persistent identity: range2215538
display id: range2215538
version: 1
direction: direction/forward
top level: BBa_M36099
start: 900
end: 1704
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2215541
display id: annotation2215541
version: 1
top level: BBa_M36099
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_M36099/annotation2215541/range2215541/1

https://synbiohub.org/public/igem/BBa_M36099/annotation2215541/range2215541/1
type: Range
persistent identity: range2215541
display id: range2215541
version: 1
direction: direction/forward
top level: BBa_M36099
start: 2435
end: 2448
orientation: inline

cloning cassette 1
type: SequenceAnnotation
persistent identity: annotation2215545
display id: annotation2215545
version: 1
top level: BBa_M36099
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36099/annotation2215545/range2215545/1

https://synbiohub.org/public/igem/BBa_M36099/annotation2215545/range2215545/1
type: Range
persistent identity: range2215545
display id: range2215545
version: 1
direction: direction/forward
top level: BBa_M36099
start: 2314
end: 2372
orientation: inline

cloning cassette 2
type: SequenceAnnotation
persistent identity: annotation2215546
display id: annotation2215546
version: 1
top level: BBa_M36099
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36099/annotation2215546/range2215546/1

https://synbiohub.org/public/igem/BBa_M36099/annotation2215546/range2215546/1
type: Range
persistent identity: range2215546
display id: range2215546
version: 1
direction: direction/forward
top level: BBa_M36099
start: 3163
end: 3221
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2215537
display id: annotation2215537
version: 1
top level: BBa_M36099
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M36099/annotation2215537/range2215537/1

https://synbiohub.org/public/igem/BBa_M36099/annotation2215537/range2215537/1
type: Range
persistent identity: range2215537
display id: range2215537
version: 1
direction: direction/forward
top level: BBa_M36099
start: 1
end: 861
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2215543
display id: annotation2215543
version: 1
top level: BBa_M36099
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_M36099/annotation2215543/range2215543/1

https://synbiohub.org/public/igem/BBa_M36099/annotation2215543/range2215543/1
type: Range
persistent identity: range2215543
display id: range2215543
version: 1
direction: direction/forward
top level: BBa_M36099
start: 3222
end: 3269
orientation: inline

rpo_bla transcription terminator
type: SequenceAnnotation
persistent identity: annotation2215539
display id: annotation2215539
version: 1
top level: BBa_M36099
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36099/annotation2215539/range2215539/1

https://synbiohub.org/public/igem/BBa_M36099/annotation2215539/range2215539/1
type: Range
persistent identity: range2215539
display id: range2215539
version: 1
direction: direction/forward
top level: BBa_M36099
start: 1705
end: 2313
orientation: inline

BBa_K1795023
type: ComponentDefinition
persistent identity: BBa K1795023
display id: BBa_K1795023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GalK Integrator Cassette
creator: Andrew Halleran, Joseph Maniaci, Caroline Golino, Taylor Jacobs, John Marken, Elli Cryan, Panya Vij, Michael LeFew
created: 2015-09-17T11:00:00Z
modified: 2015-09-17T11:34:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable description: We created a modified backbone that will allow users to quickly and easily create parts that can be integrated onto the E. coli genome. This backbone is designed as follows (where H1 refers to the first region homologous to the E. coli genome, and H2 refers to the second homologous region) In order to insert a part onto the genome, the user simply needs to put a part onto this backbone (between the prefix and suffix), and PCR up the region between (and including) H1 and H2. Users can put their parts onto this backbone with their preferred method of assembly. This part exhibits resistance to chloramphenicol and kanamycin. The parts integrated with this cassette will contain kanamycin resistance, and will be integrated in the galK region of the E. coli genome. Homologous regions modeled after those in Elowitz et al 2002.
mutable provenance: iGEM Parts Registry. Homologous regions were modeled after those used in Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002). Stochastic gene expression in a single cell. Science, 297(5584), 1183-1186.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_K1795203
component: BBa_K1795400
component: BBa_K1795024
component: BBa_K1795201
component: BBa_K1795200
component: BBa_K1795202
sequence annotation: BBa_K1795201
sequence annotation: BBa_K1795024
sequence annotation: BBa_K1795400
sequence annotation: BBa_K1795200
sequence annotation: BBa_K1795203
sequence annotation: BBa_K1795202
sequence: https://synbiohub.org/public/igem/BBa_K1795023_sequence/1

BBa_K1795203
type: Component
persistent identity: component2469225
display id: component2469225
version: 1
top level: BBa_K1795023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795203/1

BBa_K1795400
type: Component
persistent identity: component2469203
display id: component2469203
version: 1
top level: BBa_K1795023
access: public
definition: BBa_K1795400

BBa_K1795024
type: Component
persistent identity: component2469224
display id: component2469224
version: 1
top level: BBa_K1795023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795024/1

BBa_K1795201
type: Component
persistent identity: component2469206
display id: component2469206
version: 1
top level: BBa_K1795023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795201/1

BBa_K1795200
type: Component
persistent identity: component2469204
display id: component2469204
version: 1
top level: BBa_K1795023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795200/1

BBa_K1795202
type: Component
persistent identity: component2469205
display id: component2469205
version: 1
top level: BBa_K1795023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1795202/1

BBa_K1795201
type: SequenceAnnotation
persistent identity: annotation2469206
display id: annotation2469206
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469206/range2469206/1
component: BBa_K1795201

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469206/range2469206/1
type: Range
persistent identity: range2469206
display id: range2469206
version: 1
direction: direction/reverse
top level: BBa_K1795023
start: 2100
end: 2120
orientation: inline

BBa_K1795024
type: SequenceAnnotation
persistent identity: annotation2469224
display id: annotation2469224
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469224/range2469224/1
component: BBa_K1795024

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469224/range2469224/1
type: Range
persistent identity: range2469224
display id: range2469224
version: 1
direction: direction/reverse
top level: BBa_K1795023
start: 2121
end: 3299
orientation: inline

BBa_K1795400
type: SequenceAnnotation
persistent identity: annotation2469203
display id: annotation2469203
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469203/range2469203/1
component: BBa_K1795400

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469203/range2469203/1
type: Range
persistent identity: range2469203
display id: range2469203
version: 1
direction: direction/reverse
top level: BBa_K1795023
start: 1
end: 2027
orientation: inline

BBa_K1795200
type: SequenceAnnotation
persistent identity: annotation2469204
display id: annotation2469204
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469204/range2469204/1
component: BBa_K1795200

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469204/range2469204/1
type: Range
persistent identity: range2469204
display id: range2469204
version: 1
direction: direction/reverse
top level: BBa_K1795023
start: 2028
end: 2077
orientation: inline

BBa_K1795203
type: SequenceAnnotation
persistent identity: annotation2469225
display id: annotation2469225
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469225/range2469225/1
component: BBa_K1795203

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469225/range2469225/1
type: Range
persistent identity: range2469225
display id: range2469225
version: 1
direction: direction/reverse
top level: BBa_K1795023
start: 3300
end: 3349
orientation: inline

BBa_K1795202
type: SequenceAnnotation
persistent identity: annotation2469205
display id: annotation2469205
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469205/range2469205/1
component: BBa_K1795202

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469205/range2469205/1
type: Range
persistent identity: range2469205
display id: range2469205
version: 1
direction: direction/reverse
top level: BBa_K1795023
start: 2078
end: 2099
orientation: inline

BBa_K245008
type: ComponentDefinition
persistent identity: BBa K245008
display id: BBa_K245008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BB-NIC-III-HisN
creator: Nika Debeljak
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:11:38Z
discontinued: false
dominant: false
group u list: _337_
m user id: 0
owner id: 4964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This plasmid backbone is the same as BBa_K245004 except that the restriction sites AgeI and XmaI are exchanged (which results in NgoMIV and XmaI restriction sites in prefix AgeI and BspEI in suffix). This detail enables extension of part cloned into vector with SG on C-terminal or/and PG on N-terminal.
mutable notes: ccdB was multiplied from BB-NIC-II-HisN (BBa_K245004) by PCR using primers which contained restriction sites in different combinations than those found in BB-NIC-II-HisN next to ccdB. Fragment was then ligated in BB-NIC-II-HisN cut with NgoMIV and SpeI.
mutable provenance: The basis for BB-NIC-III-HisN is vector pSB1A2. Modified prefix and suffix are of synthetic origin. ccdB originates from part BBa_K245004.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: PstI
sequence annotation: NgoMIV
sequence annotation: BspEI
sequence annotation: START
sequence annotation: pBR233 origin
sequence annotation: NotI
sequence annotation: XbaI
sequence annotation: modified prefix
sequence annotation: AgeI
sequence annotation: SpeI
sequence annotation: XmaI
sequence annotation: T7 terminator
sequence annotation: EcoRI
sequence annotation: amp resistance marker
sequence annotation: amp promoter
sequence annotation: rbs
sequence annotation: lacO regulatory site
sequence annotation: His tag
sequence annotation: modified suffix
sequence annotation: STOP
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K245008_sequence/1

PstI
type: SequenceAnnotation
persistent identity: annotation2027200
display id: annotation2027200
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027200/range2027200/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027200/range2027200/1
type: Range
persistent identity: range2027200
display id: range2027200
version: 1
direction: direction/forward
top level: BBa_K245008
start: 77
end: 82
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2027213
display id: annotation2027213
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027213/range2027213/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027213/range2027213/1
type: Range
persistent identity: range2027213
display id: range2027213
version: 1
direction: direction/forward
top level: BBa_K245008
start: 2250
end: 2255
orientation: inline

BspEI
type: SequenceAnnotation
persistent identity: annotation2027195
display id: annotation2027195
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027195/range2027195/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027195/range2027195/1
type: Range
persistent identity: range2027195
display id: range2027195
version: 1
direction: direction/forward
top level: BBa_K245008
start: 7
end: 12
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2027210
display id: annotation2027210
version: 1
top level: BBa_K245008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027210/range2027210/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027210/range2027210/1
type: Range
persistent identity: range2027210
display id: range2027210
version: 1
direction: direction/forward
top level: BBa_K245008
start: 2220
end: 2222
orientation: inline

pBR233 origin
type: SequenceAnnotation
persistent identity: annotation2027201
display id: annotation2027201
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027201/range2027201/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027201/range2027201/1
type: Range
persistent identity: range2027201
display id: range2027201
version: 1
direction: direction/reverse
top level: BBa_K245008
start: 244
end: 863
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation2027199
display id: annotation2027199
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027199/range2027199/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027199/range2027199/1
type: Range
persistent identity: range2027199
display id: range2027199
version: 1
direction: direction/forward
top level: BBa_K245008
start: 70
end: 77
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2027212
display id: annotation2027212
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027212/range2027212/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027212/range2027212/1
type: Range
persistent identity: range2027212
display id: range2027212
version: 1
direction: direction/forward
top level: BBa_K245008
start: 2244
end: 2249
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation2027206
display id: annotation2027206
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027206/range2027206/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027206/range2027206/1
type: Range
persistent identity: range2027206
display id: range2027206
version: 1
direction: direction/forward
top level: BBa_K245008
start: 2125
end: 2132
orientation: inline

modified prefix
type: SequenceAnnotation
persistent identity: annotation2027205
display id: annotation2027205
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027205/range2027205/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027205/range2027205/1
type: Range
persistent identity: range2027205
display id: range2027205
version: 1
direction: direction/forward
top level: BBa_K245008
start: 2119
end: 2261
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2027193
display id: annotation2027193
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027193/range2027193/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027193/range2027193/1
type: Range
persistent identity: range2027193
display id: range2027193
version: 1
direction: direction/forward
top level: BBa_K245008
start: 1
end: 6
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2027196
display id: annotation2027196
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027196/range2027196/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027196/range2027196/1
type: Range
persistent identity: range2027196
display id: range2027196
version: 1
direction: direction/forward
top level: BBa_K245008
start: 13
end: 18
orientation: inline

XmaI
type: SequenceAnnotation
persistent identity: annotation2027214
display id: annotation2027214
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027214/range2027214/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027214/range2027214/1
type: Range
persistent identity: range2027214
display id: range2027214
version: 1
direction: direction/forward
top level: BBa_K245008
start: 2256
end: 2261
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2027198
display id: annotation2027198
version: 1
top level: BBa_K245008
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027198/range2027198/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027198/range2027198/1
type: Range
persistent identity: range2027198
display id: range2027198
version: 1
direction: direction/forward
top level: BBa_K245008
start: 22
end: 69
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2027204
display id: annotation2027204
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027204/range2027204/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027204/range2027204/1
type: Range
persistent identity: range2027204
display id: range2027204
version: 1
direction: direction/forward
top level: BBa_K245008
start: 2119
end: 2124
orientation: inline

amp resistance marker
type: SequenceAnnotation
persistent identity: annotation2027202
display id: annotation2027202
version: 1
top level: BBa_K245008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027202/range2027202/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027202/range2027202/1
type: Range
persistent identity: range2027202
display id: range2027202
version: 1
direction: direction/reverse
top level: BBa_K245008
start: 1018
end: 1878
orientation: inline

amp promoter
type: SequenceAnnotation
persistent identity: annotation2027203
display id: annotation2027203
version: 1
top level: BBa_K245008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027203/range2027203/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027203/range2027203/1
type: Range
persistent identity: range2027203
display id: range2027203
version: 1
direction: direction/reverse
top level: BBa_K245008
start: 1920
end: 1948
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2027209
display id: annotation2027209
version: 1
top level: BBa_K245008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027209/range2027209/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027209/range2027209/1
type: Range
persistent identity: range2027209
display id: range2027209
version: 1
direction: direction/forward
top level: BBa_K245008
start: 2208
end: 2214
orientation: inline

lacO regulatory site
type: SequenceAnnotation
persistent identity: annotation2027208
display id: annotation2027208
version: 1
top level: BBa_K245008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027208/range2027208/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027208/range2027208/1
type: Range
persistent identity: range2027208
display id: range2027208
version: 1
direction: direction/forward
top level: BBa_K245008
start: 2151
end: 2178
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2027211
display id: annotation2027211
version: 1
top level: BBa_K245008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027211/range2027211/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027211/range2027211/1
type: Range
persistent identity: range2027211
display id: range2027211
version: 1
direction: direction/forward
top level: BBa_K245008
start: 2226
end: 2243
orientation: inline

modified suffix
type: SequenceAnnotation
persistent identity: annotation2027194
display id: annotation2027194
version: 1
top level: BBa_K245008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027194/range2027194/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027194/range2027194/1
type: Range
persistent identity: range2027194
display id: range2027194
version: 1
direction: direction/forward
top level: BBa_K245008
start: 1
end: 82
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2027197
display id: annotation2027197
version: 1
top level: BBa_K245008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027197/range2027197/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027197/range2027197/1
type: Range
persistent identity: range2027197
display id: range2027197
version: 1
direction: direction/forward
top level: BBa_K245008
start: 19
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2027207
display id: annotation2027207
version: 1
top level: BBa_K245008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K245008/annotation2027207/range2027207/1

https://synbiohub.org/public/igem/BBa_K245008/annotation2027207/range2027207/1
type: Range
persistent identity: range2027207
display id: range2027207
version: 1
direction: direction/forward
top level: BBa_K245008
start: 2133
end: 2151
orientation: inline

BBa_K209432
type: ComponentDefinition
persistent identity: BBa K209432
display id: BBa_K209432
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCEP4 with AarI A-D sites in MCS
creator: Katja Kolar
created: 2009-10-01T11:00:00Z
modified: 2015-05-08T01:11:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _312_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K209432_sequence/1

BBa_K245006
type: ComponentDefinition
persistent identity: BBa K245006
display id: BBa_K245006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BB-NIC-II-NC
creator: Nika Debeljak
created: 2009-09-24T11:00:00Z
modified: 2015-05-08T01:11:38Z
discontinued: false
dominant: false
group u list: _337_
m user id: 0
owner id: 4964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This plasmid backbone is the same as BBa_K245004 except that it doesn't contain His tag at all.
mutable notes: First, vector BB-NIC-II-HisN was cut with EcoRI and XbaI and insert with appropriate part of prefix modified according to standard 37 without His tag was ligated in between.
mutable provenance: The basis for BB-NIC-II-NC is vector pSB1A2. Modified prefix and suffix are of synthetic origin. ccdB originates from part BBa_P1010.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: XbaI
sequence annotation: STOP
sequence annotation: XmaI
sequence annotation: modified prefix
sequence annotation: rbs
sequence annotation: T7 promoter
sequence annotation: lacO regulatory site
sequence annotation: modified suffix
sequence annotation: amp promoter
sequence annotation: amp resistance marker
sequence annotation: T7 terminator
sequence annotation: NotI
sequence annotation: NgoMIV
sequence annotation: pBR233 origin
sequence annotation: START
sequence annotation: BspEI
sequence annotation: PstI
sequence annotation: AgeI
sequence annotation: EcoRI
sequence annotation: SpeI
sequence: https://synbiohub.org/public/igem/BBa_K245006_sequence/1

XbaI
type: SequenceAnnotation
persistent identity: annotation2027041
display id: annotation2027041
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027041/range2027041/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027041/range2027041/1
type: Range
persistent identity: range2027041
display id: range2027041
version: 1
direction: direction/forward
top level: BBa_K245006
start: 2223
end: 2228
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2027028
display id: annotation2027028
version: 1
top level: BBa_K245006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027028/range2027028/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027028/range2027028/1
type: Range
persistent identity: range2027028
display id: range2027028
version: 1
direction: direction/forward
top level: BBa_K245006
start: 19
end: 21
orientation: inline

XmaI
type: SequenceAnnotation
persistent identity: annotation2027024
display id: annotation2027024
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027024/range2027024/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027024/range2027024/1
type: Range
persistent identity: range2027024
display id: range2027024
version: 1
direction: direction/forward
top level: BBa_K245006
start: 1
end: 6
orientation: inline

modified prefix
type: SequenceAnnotation
persistent identity: annotation2027044
display id: annotation2027044
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027044/range2027044/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027044/range2027044/1
type: Range
persistent identity: range2027044
display id: range2027044
version: 1
direction: direction/forward
top level: BBa_K245006
start: 2119
end: 2240
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2027039
display id: annotation2027039
version: 1
top level: BBa_K245006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027039/range2027039/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027039/range2027039/1
type: Range
persistent identity: range2027039
display id: range2027039
version: 1
direction: direction/forward
top level: BBa_K245006
start: 2208
end: 2214
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2027037
display id: annotation2027037
version: 1
top level: BBa_K245006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027037/range2027037/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027037/range2027037/1
type: Range
persistent identity: range2027037
display id: range2027037
version: 1
direction: direction/forward
top level: BBa_K245006
start: 2133
end: 2151
orientation: inline

lacO regulatory site
type: SequenceAnnotation
persistent identity: annotation2027038
display id: annotation2027038
version: 1
top level: BBa_K245006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027038/range2027038/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027038/range2027038/1
type: Range
persistent identity: range2027038
display id: range2027038
version: 1
direction: direction/forward
top level: BBa_K245006
start: 2151
end: 2178
orientation: inline

modified suffix
type: SequenceAnnotation
persistent identity: annotation2027025
display id: annotation2027025
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027025/range2027025/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027025/range2027025/1
type: Range
persistent identity: range2027025
display id: range2027025
version: 1
direction: direction/forward
top level: BBa_K245006
start: 1
end: 82
orientation: inline

amp promoter
type: SequenceAnnotation
persistent identity: annotation2027034
display id: annotation2027034
version: 1
top level: BBa_K245006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027034/range2027034/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027034/range2027034/1
type: Range
persistent identity: range2027034
display id: range2027034
version: 1
direction: direction/reverse
top level: BBa_K245006
start: 1920
end: 1948
orientation: inline

amp resistance marker
type: SequenceAnnotation
persistent identity: annotation2027033
display id: annotation2027033
version: 1
top level: BBa_K245006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027033/range2027033/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027033/range2027033/1
type: Range
persistent identity: range2027033
display id: range2027033
version: 1
direction: direction/reverse
top level: BBa_K245006
start: 1018
end: 1878
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2027029
display id: annotation2027029
version: 1
top level: BBa_K245006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027029/range2027029/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027029/range2027029/1
type: Range
persistent identity: range2027029
display id: range2027029
version: 1
direction: direction/forward
top level: BBa_K245006
start: 22
end: 69
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation2027036
display id: annotation2027036
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027036/range2027036/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027036/range2027036/1
type: Range
persistent identity: range2027036
display id: range2027036
version: 1
direction: direction/forward
top level: BBa_K245006
start: 2125
end: 2132
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2027042
display id: annotation2027042
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027042/range2027042/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027042/range2027042/1
type: Range
persistent identity: range2027042
display id: range2027042
version: 1
direction: direction/forward
top level: BBa_K245006
start: 2229
end: 2234
orientation: inline

pBR233 origin
type: SequenceAnnotation
persistent identity: annotation2027032
display id: annotation2027032
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027032/range2027032/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027032/range2027032/1
type: Range
persistent identity: range2027032
display id: range2027032
version: 1
direction: direction/reverse
top level: BBa_K245006
start: 244
end: 863
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2027040
display id: annotation2027040
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027040/range2027040/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027040/range2027040/1
type: Range
persistent identity: range2027040
display id: range2027040
version: 1
direction: direction/forward
top level: BBa_K245006
start: 2220
end: 2222
orientation: inline

BspEI
type: SequenceAnnotation
persistent identity: annotation2027026
display id: annotation2027026
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027026/range2027026/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027026/range2027026/1
type: Range
persistent identity: range2027026
display id: range2027026
version: 1
direction: direction/forward
top level: BBa_K245006
start: 7
end: 12
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2027031
display id: annotation2027031
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027031/range2027031/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027031/range2027031/1
type: Range
persistent identity: range2027031
display id: range2027031
version: 1
direction: direction/forward
top level: BBa_K245006
start: 77
end: 82
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2027043
display id: annotation2027043
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027043/range2027043/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027043/range2027043/1
type: Range
persistent identity: range2027043
display id: range2027043
version: 1
direction: direction/forward
top level: BBa_K245006
start: 2235
end: 2240
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2027035
display id: annotation2027035
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027035/range2027035/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027035/range2027035/1
type: Range
persistent identity: range2027035
display id: range2027035
version: 1
direction: direction/forward
top level: BBa_K245006
start: 2119
end: 2124
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2027027
display id: annotation2027027
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027027/range2027027/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027027/range2027027/1
type: Range
persistent identity: range2027027
display id: range2027027
version: 1
direction: direction/forward
top level: BBa_K245006
start: 13
end: 18
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation2027030
display id: annotation2027030
version: 1
top level: BBa_K245006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245006/annotation2027030/range2027030/1

https://synbiohub.org/public/igem/BBa_K245006/annotation2027030/range2027030/1
type: Range
persistent identity: range2027030
display id: range2027030
version: 1
direction: direction/forward
top level: BBa_K245006
start: 70
end: 77
orientation: inline

BBa_J72147
type: ComponentDefinition
persistent identity: BBa J72147
display id: BBa_J72147
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBjk2741
creator: Jin Huh
created: 2012-04-04T11:00:00Z
modified: 2015-05-08T01:08:28Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 3025
owning group id: 171
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: in ColE2 BseRI/AcuI Spec entry vector
mutable notes: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

BBa_K555009
type: ComponentDefinition
persistent identity: BBa K555009
display id: BBa_K555009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pYES2 - galactose-inducible expression vector for yeast
creator: Meghan Savage
created: 2011-10-04T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _723_
m user id: 0
owner id: 9577
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pYES2 yeast vector
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K555009_sequence/1

BBa_J119301
type: ComponentDefinition
persistent identity: BBa J119301
display id: BBa_J119301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scaffold Sequence for J-GGA
creator: Todd Eckdahl, Sachith Polpitiya Arachchige
created: 2013-06-10T11:00:00Z
modified: 2015-08-31T04:08:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The scaffold consist of four junctions, which allow to insert parts(promoters.RBS,Genes)between junctions A and Junction B,Junction B and Junction C or the Junction C and Junction D.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Junction C
sequence annotation: Junction D
sequence annotation: Junction B
sequence annotation: Junction A
sequence: https://synbiohub.org/public/igem/BBa_J119301_sequence/1

Junction C
type: SequenceAnnotation
persistent identity: annotation2329392
display id: annotation2329392
version: 1
top level: BBa_J119301
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J119301/annotation2329392/range2329392/1

https://synbiohub.org/public/igem/BBa_J119301/annotation2329392/range2329392/1
type: Range
persistent identity: range2329392
display id: range2329392
version: 1
direction: direction/forward
top level: BBa_J119301
start: 45
end: 61
orientation: inline

Junction D
type: SequenceAnnotation
persistent identity: annotation2329393
display id: annotation2329393
version: 1
top level: BBa_J119301
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J119301/annotation2329393/range2329393/1

https://synbiohub.org/public/igem/BBa_J119301/annotation2329393/range2329393/1
type: Range
persistent identity: range2329393
display id: range2329393
version: 1
direction: direction/forward
top level: BBa_J119301
start: 66
end: 84
orientation: inline

Junction B
type: SequenceAnnotation
persistent identity: annotation2329391
display id: annotation2329391
version: 1
top level: BBa_J119301
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J119301/annotation2329391/range2329391/1

https://synbiohub.org/public/igem/BBa_J119301/annotation2329391/range2329391/1
type: Range
persistent identity: range2329391
display id: range2329391
version: 1
direction: direction/forward
top level: BBa_J119301
start: 22
end: 39
orientation: inline

Junction A
type: SequenceAnnotation
persistent identity: annotation2329390
display id: annotation2329390
version: 1
top level: BBa_J119301
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J119301/annotation2329390/range2329390/1

https://synbiohub.org/public/igem/BBa_J119301/annotation2329390/range2329390/1
type: Range
persistent identity: range2329390
display id: range2329390
version: 1
direction: direction/forward
top level: BBa_J119301
start: 1
end: 18
orientation: inline

pSB1AT3
type: ComponentDefinition
persistent identity: pSB1AT3
display id: pSB1AT3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Tom Knight
created: 2005-06-06T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 42
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
mutable provenance: pSB1A3 with added Tet resistance
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: end of terminator
sequence annotation: APr (rev)
sequence annotation: deletion of two bases
sequence annotation: Tet-r
sequence annotation: terminator stem loop
sequence annotation: ORI
sequence annotation: E. coli his operon terminator
sequence annotation: Suffix
sequence annotation: rep (pMB1) (rev)
sequence annotation: VF2
sequence annotation: Prefix
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/pSB1AT3_sequence/1

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824010
display id: annotation1824010
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1824010/range1824010/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1824010/range1824010/1
type: Range
persistent identity: range1824010
display id: range1824010
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 3383
end: 3386
orientation: inline

APr (rev)
type: SequenceAnnotation
persistent identity: annotation1780314
display id: annotation1780314
version: 1
top level: pSB1AT3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780314/range1780314/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780314/range1780314/1
type: Range
persistent identity: range1780314
display id: range1780314
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 2324
end: 3184
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823801
display id: annotation1823801
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1823801/range1823801/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1823801/range1823801/1
type: Range
persistent identity: range1823801
display id: range1823801
version: 1
direction: direction/forward
top level: pSB1AT3
start: 34
end: 35
orientation: inline

Tet-r
type: SequenceAnnotation
persistent identity: annotation1780313
display id: annotation1780313
version: 1
top level: pSB1AT3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780313/range1780313/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780313/range1780313/1
type: Range
persistent identity: range1780313
display id: range1780313
version: 1
direction: direction/forward
top level: pSB1AT3
start: 1127
end: 2317
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780328
display id: annotation1780328
version: 1
top level: pSB1AT3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780328/range1780328/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780328/range1780328/1
type: Range
persistent identity: range1780328
display id: range1780328
version: 1
direction: direction/forward
top level: pSB1AT3
start: 30
end: 64
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1780315
display id: annotation1780315
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780315/range1780315/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780315/range1780315/1
type: Range
persistent identity: range1780315
display id: range1780315
version: 1
direction: direction/forward
top level: pSB1AT3
start: 276
end: 276
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824009
display id: annotation1824009
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1824009/range1824009/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1824009/range1824009/1
type: Range
persistent identity: range1824009
display id: range1824009
version: 1
direction: direction/forward
top level: pSB1AT3
start: 22
end: 93
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1780046
display id: annotation1780046
version: 1
top level: pSB1AT3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780046/range1780046/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780046/range1780046/1
type: Range
persistent identity: range1780046
display id: range1780046
version: 1
direction: direction/forward
top level: pSB1AT3
start: 1
end: 21
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780318
display id: annotation1780318
version: 1
top level: pSB1AT3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780318/range1780318/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780318/range1780318/1
type: Range
persistent identity: range1780318
display id: range1780318
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 3396
end: 3415
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1780066
display id: annotation1780066
version: 1
top level: pSB1AT3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780066/range1780066/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780066/range1780066/1
type: Range
persistent identity: range1780066
display id: range1780066
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 261
end: 875
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1780317
display id: annotation1780317
version: 1
top level: pSB1AT3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780317/range1780317/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780317/range1780317/1
type: Range
persistent identity: range1780317
display id: range1780317
version: 1
direction: direction/forward
top level: pSB1AT3
start: 3307
end: 3326
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1780064
display id: annotation1780064
version: 1
top level: pSB1AT3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780064/range1780064/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780064/range1780064/1
type: Range
persistent identity: range1780064
display id: range1780064
version: 1
direction: direction/forward
top level: pSB1AT3
start: 3425
end: 3446
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1780316
display id: annotation1780316
version: 1
top level: pSB1AT3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780316/range1780316/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780316/range1780316/1
type: Range
persistent identity: range1780316
display id: range1780316
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 157
end: 176
orientation: inline

pMH
type: ComponentDefinition
persistent identity: BBa K1830000
display id: BBa_K1830000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pET15BB
creator: Mathieu Husser
created: 2015-08-05T11:00:00Z
modified: 2015-10-09T10:24:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2256_
m user id: 27439
owner id: 27439
owning group id: 9
status: status/Planning
bookmark: true
mutable description: IPTG inducible E. Coli Expression vector with RCF25 compatible MCS
mutable provenance: MH
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Thrombin
sequence annotation: T7
sequence annotation: pSB1C3 backbone
sequence annotation: LacI gene
sequence annotation: TAG
sequence annotation: NgoMIV
sequence annotation: CAT gene
sequence annotation: SpeI
sequence annotation: NdeI
sequence annotation: BamHI
sequence annotation: 6X His tag
sequence annotation: ATG
sequence annotation: LacO
sequence: https://synbiohub.org/public/igem/BBa_K1830000_sequence/1

Thrombin
type: SequenceAnnotation
persistent identity: annotation2475198
display id: annotation2475198
version: 1
top level: pMH
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475198/range2475198/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475198/range2475198/1
type: Range
persistent identity: range2475198
display id: range2475198
version: 1
direction: direction/forward
top level: pMH
start: 127
end: 144
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2475204
display id: annotation2475204
version: 1
top level: pMH
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475204/range2475204/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475204/range2475204/1
type: Range
persistent identity: range2475204
display id: range2475204
version: 1
direction: direction/forward
top level: pMH
start: 241
end: 293
orientation: inline

pSB1C3 backbone
type: SequenceAnnotation
persistent identity: annotation2475207
display id: annotation2475207
version: 1
top level: pMH
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475207/range2475207/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475207/range2475207/1
type: Range
persistent identity: range2475207
display id: range2475207
version: 1
direction: direction/forward
top level: pMH
start: 291
end: 2033
orientation: inline

LacI gene
type: SequenceAnnotation
persistent identity: annotation2475206
display id: annotation2475206
version: 1
top level: pMH
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475206/range2475206/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475206/range2475206/1
type: Range
persistent identity: range2475206
display id: range2475206
version: 1
direction: direction/reverse
top level: pMH
start: 2327
end: 3529
orientation: inline

TAG
type: SequenceAnnotation
persistent identity: annotation2475209
display id: annotation2475209
version: 1
top level: pMH
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475209/range2475209/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475209/range2475209/1
type: Range
persistent identity: range2475209
display id: range2475209
version: 1
direction: direction/forward
top level: pMH
start: 241
end: 243
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2475200
display id: annotation2475200
version: 1
top level: pMH
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475200/range2475200/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475200/range2475200/1
type: Range
persistent identity: range2475200
display id: range2475200
version: 1
direction: direction/forward
top level: pMH
start: 151
end: 156
orientation: inline

CAT gene
type: SequenceAnnotation
persistent identity: annotation2475208
display id: annotation2475208
version: 1
top level: pMH
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475208/range2475208/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475208/range2475208/1
type: Range
persistent identity: range2475208
display id: range2475208
version: 1
direction: direction/forward
top level: pMH
start: 1435
end: 2092
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2475202
display id: annotation2475202
version: 1
top level: pMH
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475202/range2475202/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475202/range2475202/1
type: Range
persistent identity: range2475202
display id: range2475202
version: 1
direction: direction/forward
top level: pMH
start: 213
end: 218
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation2475210
display id: annotation2475210
version: 1
top level: pMH
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475210/range2475210/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475210/range2475210/1
type: Range
persistent identity: range2475210
display id: range2475210
version: 1
direction: direction/forward
top level: pMH
start: 145
end: 150
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2475211
display id: annotation2475211
version: 1
top level: pMH
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475211/range2475211/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475211/range2475211/1
type: Range
persistent identity: range2475211
display id: range2475211
version: 1
direction: direction/forward
top level: pMH
start: 162
end: 167
orientation: inline

6X His tag
type: SequenceAnnotation
persistent identity: annotation2475197
display id: annotation2475197
version: 1
top level: pMH
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475197/range2475197/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475197/range2475197/1
type: Range
persistent identity: range2475197
display id: range2475197
version: 1
direction: direction/forward
top level: pMH
start: 100
end: 117
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2475195
display id: annotation2475195
version: 1
top level: pMH
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475195/range2475195/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475195/range2475195/1
type: Range
persistent identity: range2475195
display id: range2475195
version: 1
direction: direction/forward
top level: pMH
start: 88
end: 90
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2475192
display id: annotation2475192
version: 1
top level: pMH
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475192/range2475192/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475192/range2475192/1
type: Range
persistent identity: range2475192
display id: range2475192
version: 1
direction: direction/forward
top level: pMH
start: 1
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2475194
display id: annotation2475194
version: 1
top level: pMH
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1830000/annotation2475194/range2475194/1

https://synbiohub.org/public/igem/BBa_K1830000/annotation2475194/range2475194/1
type: Range
persistent identity: range2475194
display id: range2475194
version: 1
direction: direction/forward
top level: pMH
start: 20
end: 42
orientation: inline

BBa_K245007
type: ComponentDefinition
persistent identity: BBa K245007
display id: BBa_K245007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BB-NIC-II-HisC
creator: Nika Debeljak
created: 2009-09-24T11:00:00Z
modified: 2015-05-08T01:11:38Z
discontinued: false
dominant: false
group u list: _337_
m user id: 0
owner id: 4964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This plasmid backbone is the same as BBa_K245004 except that it contains His tag inserted between multiple-cloning site and stop codon in suffix instead of between start codon and multiple-cloning site in prefix as in case of BBa_K245004.
mutable notes: Using overlapping primers and PCR ligation a fragment containing modified prefix, ccdB domain and modified suffix with His tag inserted between multiple-cloning site and stop codon was first obtained. The fragment was then ligated in pSB1A2 vector cut with EcoRI and PstI
mutable provenance: The basis for BB-NIC-II-HisC is vector pSB1A2. Modified prefix and suffix are of synthetic origin. ccdB originates from part BBa_P1010.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: amp resistance marker
sequence annotation: T7 promoter
sequence annotation: AgeI
sequence annotation: pBR233 origin
sequence annotation: NgoMIV
sequence annotation: PstI
sequence annotation: XmaI
sequence annotation: BspEI
sequence annotation: EcoRI
sequence annotation: XbaI
sequence annotation: SpeI
sequence annotation: START
sequence annotation: His tag
sequence annotation: amp promoter
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K245007_sequence/1

amp resistance marker
type: SequenceAnnotation
persistent identity: annotation2027077
display id: annotation2027077
version: 1
top level: BBa_K245007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027077/range2027077/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027077/range2027077/1
type: Range
persistent identity: range2027077
display id: range2027077
version: 1
direction: direction/reverse
top level: BBa_K245007
start: 981
end: 1841
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2027187
display id: annotation2027187
version: 1
top level: BBa_K245007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027187/range2027187/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027187/range2027187/1
type: Range
persistent identity: range2027187
display id: range2027187
version: 1
direction: direction/forward
top level: BBa_K245007
start: 2088
end: 2106
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2027188
display id: annotation2027188
version: 1
top level: BBa_K245007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027188/range2027188/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027188/range2027188/1
type: Range
persistent identity: range2027188
display id: range2027188
version: 1
direction: direction/forward
top level: BBa_K245007
start: 2162
end: 2167
orientation: inline

pBR233 origin
type: SequenceAnnotation
persistent identity: annotation2027076
display id: annotation2027076
version: 1
top level: BBa_K245007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027076/range2027076/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027076/range2027076/1
type: Range
persistent identity: range2027076
display id: range2027076
version: 1
direction: direction/reverse
top level: BBa_K245007
start: 207
end: 826
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2027189
display id: annotation2027189
version: 1
top level: BBa_K245007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027189/range2027189/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027189/range2027189/1
type: Range
persistent identity: range2027189
display id: range2027189
version: 1
direction: direction/forward
top level: BBa_K245007
start: 2156
end: 2161
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2027050
display id: annotation2027050
version: 1
top level: BBa_K245007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027050/range2027050/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027050/range2027050/1
type: Range
persistent identity: range2027050
display id: range2027050
version: 1
direction: direction/forward
top level: BBa_K245007
start: 40
end: 45
orientation: inline

XmaI
type: SequenceAnnotation
persistent identity: annotation2027045
display id: annotation2027045
version: 1
top level: BBa_K245007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027045/range2027045/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027045/range2027045/1
type: Range
persistent identity: range2027045
display id: range2027045
version: 1
direction: direction/forward
top level: BBa_K245007
start: 1
end: 6
orientation: inline

BspEI
type: SequenceAnnotation
persistent identity: annotation2027046
display id: annotation2027046
version: 1
top level: BBa_K245007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027046/range2027046/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027046/range2027046/1
type: Range
persistent identity: range2027046
display id: range2027046
version: 1
direction: direction/forward
top level: BBa_K245007
start: 7
end: 12
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2027191
display id: annotation2027191
version: 1
top level: BBa_K245007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027191/range2027191/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027191/range2027191/1
type: Range
persistent identity: range2027191
display id: range2027191
version: 1
direction: direction/forward
top level: BBa_K245007
start: 2082
end: 2087
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2027190
display id: annotation2027190
version: 1
top level: BBa_K245007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027190/range2027190/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027190/range2027190/1
type: Range
persistent identity: range2027190
display id: range2027190
version: 1
direction: direction/forward
top level: BBa_K245007
start: 2150
end: 2155
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2027047
display id: annotation2027047
version: 1
top level: BBa_K245007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027047/range2027047/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027047/range2027047/1
type: Range
persistent identity: range2027047
display id: range2027047
version: 1
direction: direction/forward
top level: BBa_K245007
start: 13
end: 18
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2027192
display id: annotation2027192
version: 1
top level: BBa_K245007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027192/range2027192/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027192/range2027192/1
type: Range
persistent identity: range2027192
display id: range2027192
version: 1
direction: direction/forward
top level: BBa_K245007
start: 2147
end: 2149
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2027048
display id: annotation2027048
version: 1
top level: BBa_K245007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027048/range2027048/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027048/range2027048/1
type: Range
persistent identity: range2027048
display id: range2027048
version: 1
direction: direction/forward
top level: BBa_K245007
start: 19
end: 36
orientation: inline

amp promoter
type: SequenceAnnotation
persistent identity: annotation2027186
display id: annotation2027186
version: 1
top level: BBa_K245007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027186/range2027186/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027186/range2027186/1
type: Range
persistent identity: range2027186
display id: range2027186
version: 1
direction: direction/reverse
top level: BBa_K245007
start: 1883
end: 1911
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2027049
display id: annotation2027049
version: 1
top level: BBa_K245007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K245007/annotation2027049/range2027049/1

https://synbiohub.org/public/igem/BBa_K245007/annotation2027049/range2027049/1
type: Range
persistent identity: range2027049
display id: range2027049
version: 1
direction: direction/forward
top level: BBa_K245007
start: 37
end: 39
orientation: inline

BBa_K260009
type: ComponentDefinition
persistent identity: BBa K260009
display id: BBa_K260009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMA-RQ-@TetFlp
creator: Kaj Bernhardt
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _356_
m user id: 0
owner id: 1072
owning group id: 57
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This plasmid backbone is based on pMA-RQ from Geneart. It has BioBrick prefix and suffix flanked on both sides by 70 bp of homology to pTetFlp (BBa_K260002) for tranfer of the contained BioBrick part by recombineering, for which the BioBrick needs to be excised with PvuII from the backbone. This plasmid backbone is available with BioBrick P_F3_zeoR_F3_RFP (BBa_K260017). The product of the above-described recombineering reaction BBa_K260002-BBa_K260017 is also available.
mutable notes: The 2x 70 bp homologies were designed to allow "pseudo-cloning" of a BioBrick from the present backbone '''pMA-RQ-@TetFlp''' to pTetFlp BBa_K260002 via recombineering using the PvuII fragment containing the BioBrick, where BioBrick prefix and suffix are kept untouched.
mutable provenance: Geneart plasmid pMA-RQ, with 2x 70 bp from pTetFlp BBa_K260002.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: PvuII site
sequence annotation: BBa_K260002 5' homology
sequence annotation: AmpR
sequence annotation: BBa_K260002 3' homology
sequence annotation: ColE1 origin
sequence annotation: suffix
sequence annotation: BBa_B1006 (terminator)
sequence annotation: prefix
sequence: https://synbiohub.org/public/igem/BBa_K260009_sequence/1

PvuII site
type: SequenceAnnotation
persistent identity: annotation2049652
display id: annotation2049652
version: 1
top level: BBa_K260009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K260009/annotation2049652/range2049652/1

https://synbiohub.org/public/igem/BBa_K260009/annotation2049652/range2049652/1
type: Range
persistent identity: range2049652
display id: range2049652
version: 1
direction: direction/forward
top level: BBa_K260009
start: 2496
end: 2501
orientation: inline

BBa_K260002 5' homology
type: SequenceAnnotation
persistent identity: annotation2049591
display id: annotation2049591
version: 1
top level: BBa_K260009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260009/annotation2049591/range2049591/1

https://synbiohub.org/public/igem/BBa_K260009/annotation2049591/range2049591/1
type: Range
persistent identity: range2049591
display id: range2049591
version: 1
direction: direction/forward
top level: BBa_K260009
start: 67
end: 136
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2049594
display id: annotation2049594
version: 1
top level: BBa_K260009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260009/annotation2049594/range2049594/1

https://synbiohub.org/public/igem/BBa_K260009/annotation2049594/range2049594/1
type: Range
persistent identity: range2049594
display id: range2049594
version: 1
direction: direction/reverse
top level: BBa_K260009
start: 1132
end: 1992
orientation: inline

BBa_K260002 3' homology
type: SequenceAnnotation
persistent identity: annotation2049592
display id: annotation2049592
version: 1
top level: BBa_K260009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260009/annotation2049592/range2049592/1

https://synbiohub.org/public/igem/BBa_K260009/annotation2049592/range2049592/1
type: Range
persistent identity: range2049592
display id: range2049592
version: 1
direction: direction/forward
top level: BBa_K260009
start: 2502
end: 2571
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation2049593
display id: annotation2049593
version: 1
top level: BBa_K260009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260009/annotation2049593/range2049593/1

https://synbiohub.org/public/igem/BBa_K260009/annotation2049593/range2049593/1
type: Range
persistent identity: range2049593
display id: range2049593
version: 1
direction: direction/reverse
top level: BBa_K260009
start: 317
end: 984
orientation: inline

PvuII site
type: SequenceAnnotation
persistent identity: annotation2049595
display id: annotation2049595
version: 1
top level: BBa_K260009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K260009/annotation2049595/range2049595/1

https://synbiohub.org/public/igem/BBa_K260009/annotation2049595/range2049595/1
type: Range
persistent identity: range2049595
display id: range2049595
version: 1
direction: direction/forward
top level: BBa_K260009
start: 137
end: 142
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2049534
display id: annotation2049534
version: 1
top level: BBa_K260009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260009/annotation2049534/range2049534/1

https://synbiohub.org/public/igem/BBa_K260009/annotation2049534/range2049534/1
type: Range
persistent identity: range2049534
display id: range2049534
version: 1
direction: direction/forward
top level: BBa_K260009
start: 1
end: 21
orientation: inline

BBa_B1006 (terminator)
type: SequenceAnnotation
persistent identity: annotation2049496
display id: annotation2049496
version: 1
top level: BBa_K260009
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K260009/annotation2049496/range2049496/1

https://synbiohub.org/public/igem/BBa_K260009/annotation2049496/range2049496/1
type: Range
persistent identity: range2049496
display id: range2049496
version: 1
direction: direction/forward
top level: BBa_K260009
start: 22
end: 60
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2049681
display id: annotation2049681
version: 1
top level: BBa_K260009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260009/annotation2049681/range2049681/1

https://synbiohub.org/public/igem/BBa_K260009/annotation2049681/range2049681/1
type: Range
persistent identity: range2049681
display id: range2049681
version: 1
direction: direction/forward
top level: BBa_K260009
start: 2578
end: 2599
orientation: inline

BBa_K1480008
type: ComponentDefinition
persistent identity: BBa K1480008
display id: BBa_K1480008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3 + MID, AF and BF TALEN (K1480001) Target sites
creator: Roberto Alejandro Valderrama Alfaro
created: 2014-10-15T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1860_
m user id: 0
owner id: 19018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Backbone vector of pSB1C3 tha contains a modification with a TALEN target site on the center, before and after the origin of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains three TALEN target sites (MID+AF+BF)
mutable notes: Modifications were inserted by PCR reactions in both strands.
mutable provenance: Derived from plasmid pSB1C3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1480008_sequence/1

pSB4T5
type: ComponentDefinition
persistent identity: pSB4T5
display id: pSB4T5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4T3 is a low copy plasmid with tetracycline resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is constructed from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design for more information.
mutable provenance: BBa_I51001 vector scaffold, BBa_I50040 replication origin and BBa_P1005 resistance marker.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00000
component: BBa_B0053
component: BBa_B0043
component: BBa_B0042
component: BBa_G00001
component: BBa_B0055
component: BBa_B0062
component: BBa_B0044
component: BBa_G00100
component: BBa_G00102
component: BBa_G0003
component: BBa_B0054
component: BBa_G0002
component: BBa_P1005
component: BBa_I50042
component: BBa_G0004
sequence annotation: BBa_G00000
sequence annotation: BBa_G00001
sequence annotation: BBa_B0042
sequence annotation: BBa_B0062
sequence annotation: BBa_P1005
sequence annotation: BBa_G00102
sequence annotation: BBa_I50042
sequence annotation: BBa_B0043
sequence annotation: BBa_G0002
sequence annotation: BBa_G00100
sequence annotation: BBa_G0004
sequence annotation: BBa_B0053
sequence annotation: BBa_B0055
sequence annotation: BBa_G0003
sequence annotation: BBa_B0054
sequence annotation: BBa_B0044
sequence: https://synbiohub.org/public/igem/pSB4T5_sequence/1

BBa_G00000
type: Component
persistent identity: component2236478
display id: component2236478
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0053
type: Component
persistent identity: component2236456
display id: component2236456
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0043
type: Component
persistent identity: component2236473
display id: component2236473
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component2236468
display id: component2236468
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00001
type: Component
persistent identity: component2236401
display id: component2236401
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0055
type: Component
persistent identity: component2236461
display id: component2236461
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0062
type: Component
persistent identity: component2236420
display id: component2236420
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0044
type: Component
persistent identity: component2236406
display id: component2236406
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00100
type: Component
persistent identity: component2236458
display id: component2236458
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00102
type: Component
persistent identity: component2236418
display id: component2236418
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G0003
type: Component
persistent identity: component2236421
display id: component2236421
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0054
type: Component
persistent identity: component2236416
display id: component2236416
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G0002
type: Component
persistent identity: component2236450
display id: component2236450
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_P1005
type: Component
persistent identity: component2236453
display id: component2236453
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1005/1

BBa_I50042
type: Component
persistent identity: component2236449
display id: component2236449
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0042
type: Component
persistent identity: component2236413
display id: component2236413
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0004
type: Component
persistent identity: component2236454
display id: component2236454
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236478
display id: annotation2236478
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236478/range2236478/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4T5/annotation2236478/range2236478/1
type: Range
persistent identity: range2236478
display id: range2236478
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3714
end: 3735
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236401
display id: annotation2236401
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236401/range2236401/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4T5/annotation2236401/range2236401/1
type: Range
persistent identity: range2236401
display id: range2236401
version: 1
direction: direction/reverse
top level: pSB4T5
start: 1
end: 21
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236468
display id: annotation2236468
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236468/range2236468/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4T5/annotation2236468/range2236468/1
type: Range
persistent identity: range2236468
display id: range2236468
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3689
end: 3700
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236420
display id: annotation2236420
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236420/range2236420/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4T5/annotation2236420/range2236420/1
type: Range
persistent identity: range2236420
display id: range2236420
version: 1
direction: direction/reverse
top level: pSB4T5
start: 136
end: 176
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236413
display id: annotation2236413
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236413/range2236413/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4T5/annotation2236413/range2236413/1
type: Range
persistent identity: range2236413
display id: range2236413
version: 1
direction: direction/reverse
top level: pSB4T5
start: 35
end: 46
orientation: inline

BBa_P1005
type: SequenceAnnotation
persistent identity: annotation2236453
display id: annotation2236453
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236453/range2236453/1
component: BBa_P1005

https://synbiohub.org/public/igem/pSB4T5/annotation2236453/range2236453/1
type: Range
persistent identity: range2236453
display id: range2236453
version: 1
direction: direction/reverse
top level: pSB4T5
start: 2229
end: 3511
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236418
display id: annotation2236418
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236418/range2236418/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4T5/annotation2236418/range2236418/1
type: Range
persistent identity: range2236418
display id: range2236418
version: 1
direction: direction/reverse
top level: pSB4T5
start: 116
end: 135
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236449
display id: annotation2236449
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236449/range2236449/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4T5/annotation2236449/range2236449/1
type: Range
persistent identity: range2236449
display id: range2236449
version: 1
direction: direction/reverse
top level: pSB4T5
start: 184
end: 2220
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236473
display id: annotation2236473
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236473/range2236473/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4T5/annotation2236473/range2236473/1
type: Range
persistent identity: range2236473
display id: range2236473
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3701
end: 3713
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236450
display id: annotation2236450
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236450/range2236450/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4T5/annotation2236450/range2236450/1
type: Range
persistent identity: range2236450
display id: range2236450
version: 1
direction: direction/reverse
top level: pSB4T5
start: 2221
end: 2228
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236458
display id: annotation2236458
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236458/range2236458/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4T5/annotation2236458/range2236458/1
type: Range
persistent identity: range2236458
display id: range2236458
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3591
end: 3610
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236454
display id: annotation2236454
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236454/range2236454/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4T5/annotation2236454/range2236454/1
type: Range
persistent identity: range2236454
display id: range2236454
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3512
end: 3518
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236456
display id: annotation2236456
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236456/range2236456/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4T5/annotation2236456/range2236456/1
type: Range
persistent identity: range2236456
display id: range2236456
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3519
end: 3590
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236461
display id: annotation2236461
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236461/range2236461/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4T5/annotation2236461/range2236461/1
type: Range
persistent identity: range2236461
display id: range2236461
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3611
end: 3688
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236421
display id: annotation2236421
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236421/range2236421/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4T5/annotation2236421/range2236421/1
type: Range
persistent identity: range2236421
display id: range2236421
version: 1
direction: direction/reverse
top level: pSB4T5
start: 177
end: 183
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236416
display id: annotation2236416
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236416/range2236416/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4T5/annotation2236416/range2236416/1
type: Range
persistent identity: range2236416
display id: range2236416
version: 1
direction: direction/reverse
top level: pSB4T5
start: 47
end: 115
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236406
display id: annotation2236406
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236406/range2236406/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4T5/annotation2236406/range2236406/1
type: Range
persistent identity: range2236406
display id: range2236406
version: 1
direction: direction/reverse
top level: pSB4T5
start: 22
end: 34
orientation: inline

BBa_K864002
type: ComponentDefinition
persistent identity: BBa K864002
display id: BBa_K864002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: Lambda Red forward primer
sequence annotation: VF2
sequence annotation: Lux terminator
sequence annotation: KanR BBa_P1003
sequence annotation: Lambda Red reverse primer
sequence annotation: P22 late terminator
sequence annotation: TonB terminator
sequence annotation: VR
sequence annotation: T3 terminator
sequence annotation: rrnC terminator
sequence: https://synbiohub.org/public/igem/BBa_K864002_sequence/1

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2198835
display id: annotation2198835
version: 1
top level: BBa_K864002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198835/range2198835/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198835/range2198835/1
type: Range
persistent identity: range2198835
display id: range2198835
version: 1
direction: direction/forward
top level: BBa_K864002
start: 190
end: 2405
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198836
display id: annotation2198836
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198836/range2198836/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198836/range2198836/1
type: Range
persistent identity: range2198836
display id: range2198836
version: 1
direction: direction/forward
top level: BBa_K864002
start: 2433
end: 2450
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198840
display id: annotation2198840
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198840/range2198840/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198840/range2198840/1
type: Range
persistent identity: range2198840
display id: range2198840
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3519
end: 3538
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198837
display id: annotation2198837
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198837/range2198837/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198837/range2198837/1
type: Range
persistent identity: range2198837
display id: range2198837
version: 1
direction: direction/reverse
top level: BBa_K864002
start: 2454
end: 2499
orientation: inline

KanR BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2198838
display id: annotation2198838
version: 1
top level: BBa_K864002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198838/range2198838/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198838/range2198838/1
type: Range
persistent identity: range2198838
display id: range2198838
version: 1
direction: direction/forward
top level: BBa_K864002
start: 2506
end: 3224
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198831
display id: annotation2198831
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198831/range2198831/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198831/range2198831/1
type: Range
persistent identity: range2198831
display id: range2198831
version: 1
direction: direction/reverse
top level: BBa_K864002
start: 18
end: 38
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198839
display id: annotation2198839
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198839/range2198839/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198839/range2198839/1
type: Range
persistent identity: range2198839
display id: range2198839
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3478
end: 3518
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2198832
display id: annotation2198832
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198832/range2198832/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198832/range2198832/1
type: Range
persistent identity: range2198832
display id: range2198832
version: 1
direction: direction/forward
top level: BBa_K864002
start: 47
end: 115
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198833
display id: annotation2198833
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198833/range2198833/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198833/range2198833/1
type: Range
persistent identity: range2198833
display id: range2198833
version: 1
direction: direction/forward
top level: BBa_K864002
start: 116
end: 135
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198841
display id: annotation2198841
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198841/range2198841/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198841/range2198841/1
type: Range
persistent identity: range2198841
display id: range2198841
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3539
end: 3616
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198834
display id: annotation2198834
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198834/range2198834/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198834/range2198834/1
type: Range
persistent identity: range2198834
display id: range2198834
version: 1
direction: direction/forward
top level: BBa_K864002
start: 136
end: 175
orientation: inline

BBa_K1480015
type: ComponentDefinition
persistent identity: BBa K1480015
display id: BBa_K1480015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB3C5 + Before TALEN (K1480001) Target site
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Backbone vector of pSB3C5 that contains a single modification with a TALEN target site after the origin of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains a single TALEN target site (AF).
mutable notes: Modifications where inserted by PCR on both directions due to TALEN specificity.
mutable provenance: Derived from pSB3C5 plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1480015_sequence/1

BBa_K1678008
type: ComponentDefinition
persistent identity: BBa K1678008
display id: BBa_K1678008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A biobrick shuttle plasmid working between Lactobacillus plantarum, and E.coli with erythromycin res
creator: Prateek Garg
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T05:53:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2096_
m user id: 27494
owner id: 27494
owning group id: 9
status: status/Planning
bookmark: false
mutable description: It's a biobrick plasmid with biobrick assembly restriction sites, a fused origin of replication between gram positive and negative cells, and an erythromycin resistance.
mutable notes: A part of pSIP409 was extracted with tails and re-circularized.
mutable provenance: It's been made from the plasmid pSIP409
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1678008_sequence/1

Silver vec
type: ComponentDefinition
persistent identity: BBa J63009
display id: BBa_J63009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy protein fusion vector (Silver lab standard)
creator: Caroline Ajo-Franklin
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Silver Lab alias V0002. This is a modified biobrick vector (fusion brick vector) that contains the specified EcoRI, SpeI, XbaI, and PstI sites. The only difference is that the part sequence immediately follows the XbaI site and immediately precedes the SpeI site. This change allows in-frame fusions of protein coding regions to be made using standard biobricks assembly methods.
mutable notes: If the part starts with the nucleotides tc, then that part-containing plasmid must be grow in a DamI- strain to avoid methylation (and inhibition of digestion) at the XbaI site.
mutable provenance: based on pUC19 plasmid
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rep pMB1 replication origin
sequence annotation: bla(P3) promoter -35 sequence
sequence annotation: bla gene
sequence annotation: modified BioBrick prefix
sequence annotation: RNAI transcript
sequence annotation: RNAII transcript
sequence annotation: modified BioBrick suffix
sequence annotation: beta-lactamase (bla gene)
sequence annotation: bla(P3) promoter -10 sequence
sequence: https://synbiohub.org/public/igem/BBa_J63009_sequence/1

rep pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation1969661
display id: annotation1969661
version: 1
top level: Silver vec
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969661/range1969661/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969661/range1969661/1
type: Range
persistent identity: range1969661
display id: range1969661
version: 1
direction: direction/reverse
top level: Silver vec
start: 182
end: 796
orientation: inline

bla(P3) promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969666
display id: annotation1969666
version: 1
top level: Silver vec
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969666/range1969666/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969666/range1969666/1
type: Range
persistent identity: range1969666
display id: range1969666
version: 1
direction: direction/reverse
top level: Silver vec
start: 1881
end: 1886
orientation: inline

bla gene
type: SequenceAnnotation
persistent identity: annotation1969664
display id: annotation1969664
version: 1
top level: Silver vec
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969664/range1969664/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969664/range1969664/1
type: Range
persistent identity: range1969664
display id: range1969664
version: 1
direction: direction/reverse
top level: Silver vec
start: 1824
end: 1828
orientation: inline

modified BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969667
display id: annotation1969667
version: 1
top level: Silver vec
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969667/range1969667/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969667/range1969667/1
type: Range
persistent identity: range1969667
display id: range1969667
version: 1
direction: direction/forward
top level: Silver vec
start: 2057
end: 2077
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1969662
display id: annotation1969662
version: 1
top level: Silver vec
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969662/range1969662/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969662/range1969662/1
type: Range
persistent identity: range1969662
display id: range1969662
version: 1
direction: direction/forward
top level: Silver vec
start: 639
end: 746
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1969660
display id: annotation1969660
version: 1
top level: Silver vec
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969660/range1969660/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969660/range1969660/1
type: Range
persistent identity: range1969660
display id: range1969660
version: 1
direction: direction/reverse
top level: Silver vec
start: 197
end: 749
orientation: inline

modified BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969659
display id: annotation1969659
version: 1
top level: Silver vec
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969659/range1969659/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969659/range1969659/1
type: Range
persistent identity: range1969659
display id: range1969659
version: 1
direction: direction/forward
top level: Silver vec
start: 1
end: 20
orientation: inline

beta-lactamase (bla gene)
type: SequenceAnnotation
persistent identity: annotation1969663
display id: annotation1969663
version: 1
top level: Silver vec
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969663/range1969663/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969663/range1969663/1
type: Range
persistent identity: range1969663
display id: range1969663
version: 1
direction: direction/reverse
top level: Silver vec
start: 956
end: 1816
orientation: inline

bla(P3) promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969665
display id: annotation1969665
version: 1
top level: Silver vec
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969665/range1969665/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969665/range1969665/1
type: Range
persistent identity: range1969665
display id: range1969665
version: 1
direction: direction/reverse
top level: Silver vec
start: 1860
end: 1865
orientation: inline

BBa_K394001
type: ComponentDefinition
persistent identity: BBa K394001
display id: BBa_K394001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid for Chromosomal Integration in Yeast at His3
creator: Brian Landry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part is designed to allow simple chromosomal integration of BioBrick plasmids. It is based off of the bacterial pSB1AT3 backbone allowing for normal DNA manipulation to occur in bacteria. However the BioBrick cut sites are flanked by homologous regions to the His3 locus in S. cerevisiae. To transform S. cerevisiae the plasmid is cut with BbsI creating two non-sticky ends. The linear fragment then contains His3 homologous sequences at its ends that can be used to chromosomally integrate the BioBrick plasmid into the yeast genome. Transformants can then be selected for using His- medium.
mutable notes: None
mutable provenance: This part was derived from plasmid pSB1AT3. Two successive rounds of PCR inserted a BbsI cut site and 40 bp homologous region in before the prefix and after the suffix. The following Primers were used: Forward 1 GCACAGAACAATAACCTGCTGGAAACGAAGATAAATCgaagacGATTACTTCGCGTTATGCAGGC Reverse 1 GCATCTTCTCAAATATGCTTCCCAGCCTGCTTATCcttctgAAATTCTGCCTCGTGATACGCC Forward 2 tactagtagcggccgctgcagTCTTAACCCAACTGCACAGAACAATAACCTGCTGGAAACG Reverse 2 ctctagaagcggccgcgaattcTTAGTATTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: AmpR (rev)
sequence annotation: His3 homology
sequence annotation: BioBrick Prefix
sequence annotation: rep (pMB1) (rev)
sequence annotation: BbsI Cut Site
sequence annotation: VF2
sequence annotation: Tet-R
sequence annotation: BioBrick Suffix
sequence annotation: ORI
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_K394001_sequence/1

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation2102638
display id: annotation2102638
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102638/range2102638/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102638/range2102638/1
type: Range
persistent identity: range2102638
display id: range2102638
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 2324
end: 3184
orientation: inline

His3 homology
type: SequenceAnnotation
persistent identity: annotation2102389
display id: annotation2102389
version: 1
top level: BBa_K394001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102389/range2102389/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102389/range2102389/1
type: Range
persistent identity: range2102389
display id: range2102389
version: 1
direction: direction/forward
top level: BBa_K394001
start: 22
end: 69
orientation: inline

BioBrick Prefix
type: SequenceAnnotation
persistent identity: annotation2102649
display id: annotation2102649
version: 1
top level: BBa_K394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102649/range2102649/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102649/range2102649/1
type: Range
persistent identity: range2102649
display id: range2102649
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3435
end: 3457
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2102598
display id: annotation2102598
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102598/range2102598/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102598/range2102598/1
type: Range
persistent identity: range2102598
display id: range2102598
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 261
end: 875
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104026
display id: annotation2104026
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2104026/range2104026/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2104026/range2104026/1
type: Range
persistent identity: range2104026
display id: range2104026
version: 1
direction: direction/forward
top level: BBa_K394001
start: 71
end: 76
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2102640
display id: annotation2102640
version: 1
top level: BBa_K394001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102640/range2102640/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102640/range2102640/1
type: Range
persistent identity: range2102640
display id: range2102640
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3307
end: 3326
orientation: inline

Tet-R
type: SequenceAnnotation
persistent identity: annotation2102637
display id: annotation2102637
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102637/range2102637/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102637/range2102637/1
type: Range
persistent identity: range2102637
display id: range2102637
version: 1
direction: direction/forward
top level: BBa_K394001
start: 1127
end: 2317
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104028
display id: annotation2104028
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2104028/range2104028/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2104028/range2104028/1
type: Range
persistent identity: range2104028
display id: range2104028
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 3379
end: 3385
orientation: inline

BioBrick Suffix
type: SequenceAnnotation
persistent identity: annotation2090942
display id: annotation2090942
version: 1
top level: BBa_K394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2090942/range2090942/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2090942/range2090942/1
type: Range
persistent identity: range2090942
display id: range2090942
version: 1
direction: direction/forward
top level: BBa_K394001
start: 1
end: 21
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2102635
display id: annotation2102635
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102635/range2102635/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102635/range2102635/1
type: Range
persistent identity: range2102635
display id: range2102635
version: 1
direction: direction/forward
top level: BBa_K394001
start: 276
end: 276
orientation: inline

His3 homology
type: SequenceAnnotation
persistent identity: annotation2102645
display id: annotation2102645
version: 1
top level: BBa_K394001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102645/range2102645/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102645/range2102645/1
type: Range
persistent identity: range2102645
display id: range2102645
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3386
end: 3435
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2102390
display id: annotation2102390
version: 1
top level: BBa_K394001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102390/range2102390/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102390/range2102390/1
type: Range
persistent identity: range2102390
display id: range2102390
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 157
end: 176
orientation: inline

BBa_K415049
type: ComponentDefinition
persistent identity: BBa K415049
display id: BBa_K415049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cm Backbone with pBR322 Derived Origin
creator: Shawn Pan
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
group u list: _525_
m user id: 0
owner id: 6742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a biobrick backbone with chloramphenicol resistance and pBR322 derived origin. The copy number is expected to be around 20. The characterization of this part is currently happening, and more data will be uploaded soon. Note: The backbone contains an extra VR sequencing site from the origin K415039. This site will be removed in the final version pSB6C5. Also, cells containing this plasmid grow slower than usual.
mutable notes: The extra reverse sequencing site needs to be removed in the final version (pSB6C5). The resistance P1004 was placed before the origin operon K415039 to provide a promoter to the copy-number regulating protein ROP.
mutable provenance: This backbone was constructed with the standard biobrick plasmid construction method. The origin is K415039 and the chloramphenicol resistance is from P1004. After several failures in getting the correct colonies with the ccdB containing base vector I51020, the appropriate base vector region was obtained by PCR of an existing version 5 backbone containing RFP (pSB3T5). The RFP version of the base vector allowed for easy screening for correct colonies.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RBS
sequence annotation: pMB1 Origin
sequence annotation: Extra VR site
sequence annotation: Biobrick Prefix
sequence annotation: VR Site
sequence annotation: ROP
sequence annotation: P1004 (Cm resistance)
sequence annotation: Biobrick Suffix
sequence annotation: VF2 Site
sequence: https://synbiohub.org/public/igem/BBa_K415049_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2103815
display id: annotation2103815
version: 1
top level: BBa_K415049
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K415049/annotation2103815/range2103815/1

https://synbiohub.org/public/igem/BBa_K415049/annotation2103815/range2103815/1
type: Range
persistent identity: range2103815
display id: range2103815
version: 1
direction: direction/forward
top level: BBa_K415049
start: 969
end: 974
orientation: inline

pMB1 Origin
type: SequenceAnnotation
persistent identity: annotation2103810
display id: annotation2103810
version: 1
top level: BBa_K415049
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K415049/annotation2103810/range2103810/1

https://synbiohub.org/public/igem/BBa_K415049/annotation2103810/range2103810/1
type: Range
persistent identity: range2103810
display id: range2103810
version: 1
direction: direction/forward
top level: BBa_K415049
start: 1583
end: 2197
orientation: inline

Extra VR site
type: SequenceAnnotation
persistent identity: annotation2103801
display id: annotation2103801
version: 1
top level: BBa_K415049
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K415049/annotation2103801/range2103801/1

https://synbiohub.org/public/igem/BBa_K415049/annotation2103801/range2103801/1
type: Range
persistent identity: range2103801
display id: range2103801
version: 1
direction: direction/reverse
top level: BBa_K415049
start: 1479
end: 1498
orientation: inline

Biobrick Prefix
type: SequenceAnnotation
persistent identity: annotation2103795
display id: annotation2103795
version: 1
top level: BBa_K415049
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K415049/annotation2103795/range2103795/1

https://synbiohub.org/public/igem/BBa_K415049/annotation2103795/range2103795/1
type: Range
persistent identity: range2103795
display id: range2103795
version: 1
direction: direction/forward
top level: BBa_K415049
start: 2405
end: 2426
orientation: inline

VR Site
type: SequenceAnnotation
persistent identity: annotation2103804
display id: annotation2103804
version: 1
top level: BBa_K415049
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K415049/annotation2103804/range2103804/1

https://synbiohub.org/public/igem/BBa_K415049/annotation2103804/range2103804/1
type: Range
persistent identity: range2103804
display id: range2103804
version: 1
direction: direction/reverse
top level: BBa_K415049
start: 116
end: 135
orientation: inline

ROP
type: SequenceAnnotation
persistent identity: annotation2103813
display id: annotation2103813
version: 1
top level: BBa_K415049
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K415049/annotation2103813/range2103813/1

https://synbiohub.org/public/igem/BBa_K415049/annotation2103813/range2103813/1
type: Range
persistent identity: range2103813
display id: range2103813
version: 1
direction: direction/forward
top level: BBa_K415049
start: 979
end: 1172
orientation: inline

P1004 (Cm resistance)
type: SequenceAnnotation
persistent identity: annotation2103809
display id: annotation2103809
version: 1
top level: BBa_K415049
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K415049/annotation2103809/range2103809/1

https://synbiohub.org/public/igem/BBa_K415049/annotation2103809/range2103809/1
type: Range
persistent identity: range2103809
display id: range2103809
version: 1
direction: direction/forward
top level: BBa_K415049
start: 184
end: 952
orientation: inline

Biobrick Suffix
type: SequenceAnnotation
persistent identity: annotation2103799
display id: annotation2103799
version: 1
top level: BBa_K415049
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K415049/annotation2103799/range2103799/1

https://synbiohub.org/public/igem/BBa_K415049/annotation2103799/range2103799/1
type: Range
persistent identity: range2103799
display id: range2103799
version: 1
direction: direction/forward
top level: BBa_K415049
start: 1
end: 21
orientation: inline

VF2 Site
type: SequenceAnnotation
persistent identity: annotation2103807
display id: annotation2103807
version: 1
top level: BBa_K415049
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K415049/annotation2103807/range2103807/1

https://synbiohub.org/public/igem/BBa_K415049/annotation2103807/range2103807/1
type: Range
persistent identity: range2103807
display id: range2103807
version: 1
direction: direction/forward
top level: BBa_K415049
start: 2281
end: 2301
orientation: inline

BBa_K563053
type: ComponentDefinition
persistent identity: BBa K563053
display id: BBa_K563053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
creator: Guo Jing
created: 2011-10-04T11:00:00Z
modified: 2015-05-08T01:12:42Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _731_
m user id: 0
owner id: 10236
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pYES2 is a 5.9 kb vector for inducible expression of recombinant proteins in S.cerevisivae. Feature of the vector allow easy cloning of your gene of interest and selection of transformants by uracil prototrophy. The vector contains the follow elements:
mutable notes: We use this vector for expressing mutant Tor2. In order to operate easily, we separate Tor2 into three parts. So we reconstruct the pYE by adding specific restriction sites for linking.
mutable provenance: we bought it from invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: CYC1 transcription terminator
sequence annotation: GAL1
sequence annotation: pUC origin
sequence annotation: f1 origin
sequence annotation: URA3 gene
sequence annotation: Amplicillin
sequence: https://synbiohub.org/public/igem/BBa_K563053_sequence/1

CYC1 transcription terminator
type: SequenceAnnotation
persistent identity: annotation2153264
display id: annotation2153264
version: 1
top level: BBa_K563053
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K563053/annotation2153264/range2153264/1

https://synbiohub.org/public/igem/BBa_K563053/annotation2153264/range2153264/1
type: Range
persistent identity: range2153264
display id: range2153264
version: 1
direction: direction/forward
top level: BBa_K563053
start: 608
end: 856
orientation: inline

GAL1
type: SequenceAnnotation
persistent identity: annotation2153262
display id: annotation2153262
version: 1
top level: BBa_K563053
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K563053/annotation2153262/range2153262/1

https://synbiohub.org/public/igem/BBa_K563053/annotation2153262/range2153262/1
type: Range
persistent identity: range2153262
display id: range2153262
version: 1
direction: direction/forward
top level: BBa_K563053
start: 1
end: 451
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2153329
display id: annotation2153329
version: 1
top level: BBa_K563053
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K563053/annotation2153329/range2153329/1

https://synbiohub.org/public/igem/BBa_K563053/annotation2153329/range2153329/1
type: Range
persistent identity: range2153329
display id: range2153329
version: 1
direction: direction/forward
top level: BBa_K563053
start: 1038
end: 1711
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2153331
display id: annotation2153331
version: 1
top level: BBa_K563053
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K563053/annotation2153331/range2153331/1

https://synbiohub.org/public/igem/BBa_K563053/annotation2153331/range2153331/1
type: Range
persistent identity: range2153331
display id: range2153331
version: 1
direction: direction/forward
top level: BBa_K563053
start: 5384
end: 5839
orientation: inline

URA3 gene
type: SequenceAnnotation
persistent identity: annotation2153279
display id: annotation2153279
version: 1
top level: BBa_K563053
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K563053/annotation2153279/range2153279/1

https://synbiohub.org/public/igem/BBa_K563053/annotation2153279/range2153279/1
type: Range
persistent identity: range2153279
display id: range2153279
version: 1
direction: direction/forward
top level: BBa_K563053
start: 2734
end: 3841
orientation: inline

Amplicillin
type: SequenceAnnotation
persistent identity: annotation2153278
display id: annotation2153278
version: 1
top level: BBa_K563053
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K563053/annotation2153278/range2153278/1

https://synbiohub.org/public/igem/BBa_K563053/annotation2153278/range2153278/1
type: Range
persistent identity: range2153278
display id: range2153278
version: 1
direction: direction/forward
top level: BBa_K563053
start: 1856
end: 2716
orientation: inline

BBa_K1138000
type: ComponentDefinition
persistent identity: BBa K1138000
display id: BBa_K1138000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAW42-gs (ugaiGEM2)
creator: Rachit Jain
created: 2013-09-05T11:00:00Z
modified: 2015-05-08T01:09:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1450_
m user id: 0
owner id: 12767
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The plasmid pAW42-gs(ugaiGEM2)consists of the gs gene inserted into the backbone of pAW42-vector. The gs gene encodes for geraniol synthase enzyme. This vector has puramycin marker for selection in Methanogens and ampicilin marker for selection in E. coli.
mutable notes: Codon optimized for expression in Methanococcus.
mutable provenance: The gs gene was obtained from Ocimum basilicum.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: puramycin
sequence annotation: promoter
sequence annotation: ampicillin
sequence annotation: gs gene sequence
sequence: https://synbiohub.org/public/igem/BBa_K1138000_sequence/1

puramycin
type: SequenceAnnotation
persistent identity: annotation2335209
display id: annotation2335209
version: 1
top level: BBa_K1138000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138000/annotation2335209/range2335209/1

https://synbiohub.org/public/igem/BBa_K1138000/annotation2335209/range2335209/1
type: Range
persistent identity: range2335209
display id: range2335209
version: 1
direction: direction/forward
top level: BBa_K1138000
start: 2681
end: 3280
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2335211
display id: annotation2335211
version: 1
top level: BBa_K1138000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1138000/annotation2335211/range2335211/1

https://synbiohub.org/public/igem/BBa_K1138000/annotation2335211/range2335211/1
type: Range
persistent identity: range2335211
display id: range2335211
version: 1
direction: direction/forward
top level: BBa_K1138000
start: 1
end: 160
orientation: inline

ampicillin
type: SequenceAnnotation
persistent identity: annotation2335210
display id: annotation2335210
version: 1
top level: BBa_K1138000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138000/annotation2335210/range2335210/1

https://synbiohub.org/public/igem/BBa_K1138000/annotation2335210/range2335210/1
type: Range
persistent identity: range2335210
display id: range2335210
version: 1
direction: direction/forward
top level: BBa_K1138000
start: 4063
end: 4923
orientation: inline

gs gene sequence
type: SequenceAnnotation
persistent identity: annotation2335208
display id: annotation2335208
version: 1
top level: BBa_K1138000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1138000/annotation2335208/range2335208/1

https://synbiohub.org/public/igem/BBa_K1138000/annotation2335208/range2335208/1
type: Range
persistent identity: range2335208
display id: range2335208
version: 1
direction: direction/forward
top level: BBa_K1138000
start: 166
end: 1870
orientation: inline

BBa_K1110001
type: ComponentDefinition
persistent identity: BBa K1110001
display id: BBa_K1110001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAPzalphaC+standardizing linker
creator: Lydia Studdard
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1421_
m user id: 0
owner id: 16826
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The plasmid pGAPzalphaC was standardized according to iGEM standards using a linker piece
mutable notes: NOne
mutable provenance: pGAPzalphC, Saccharomyces cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1110001_sequence/1

pSB1C3
type: ComponentDefinition
persistent identity: pSB1C3
display id: pSB1C3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Austin Che
created: 2008-09-07T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.
mutable notes: Eliminated ampicillin resistance.
mutable provenance: This part was derived from pSB1AC3-P1010.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Chloramphenicol resistance
sequence annotation: T0 terminator
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: ORI
sequence annotation: BioBrick prefix
sequence annotation: Stem loop
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: end of terminator
sequence annotation: E. coli his operon terminator
sequence annotation: rep (pMB1)
sequence annotation: BioBrick suffix
sequence: https://synbiohub.org/public/igem/pSB1C3_sequence/1

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2119045
display id: annotation2119045
version: 1
top level: pSB1C3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119045/range2119045/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119045/range2119045/1
type: Range
persistent identity: range2119045
display id: range2119045
version: 1
direction: direction/reverse
top level: pSB1C3
start: 1160
end: 1819
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2119044
display id: annotation2119044
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119044/range2119044/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119044/range2119044/1
type: Range
persistent identity: range2119044
display id: range2119044
version: 1
direction: direction/forward
top level: pSB1C3
start: 1042
end: 1147
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation2119041
display id: annotation2119041
version: 1
top level: pSB1C3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119041/range2119041/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119041/range2119041/1
type: Range
persistent identity: range2119041
display id: range2119041
version: 1
direction: direction/reverse
top level: pSB1C3
start: 157
end: 176
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2119043
display id: annotation2119043
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119043/range2119043/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119043/range2119043/1
type: Range
persistent identity: range2119043
display id: range2119043
version: 1
direction: direction/forward
top level: pSB1C3
start: 276
end: 276
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2119049
display id: annotation2119049
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119049/range2119049/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119049/range2119049/1
type: Range
persistent identity: range2119049
display id: range2119049
version: 1
direction: direction/forward
top level: pSB1C3
start: 2051
end: 2072
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2119048
display id: annotation2119048
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119048/range2119048/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119048/range2119048/1
type: Range
persistent identity: range2119048
display id: range2119048
version: 1
direction: direction/reverse
top level: pSB1C3
start: 2022
end: 2041
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation2119046
display id: annotation2119046
version: 1
top level: pSB1C3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119046/range2119046/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119046/range2119046/1
type: Range
persistent identity: range2119046
display id: range2119046
version: 1
direction: direction/forward
top level: pSB1C3
start: 1933
end: 1952
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2119047
display id: annotation2119047
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119047/range2119047/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119047/range2119047/1
type: Range
persistent identity: range2119047
display id: range2119047
version: 1
direction: direction/reverse
top level: pSB1C3
start: 2009
end: 2012
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2119039
display id: annotation2119039
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119039/range2119039/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119039/range2119039/1
type: Range
persistent identity: range2119039
display id: range2119039
version: 1
direction: direction/forward
top level: pSB1C3
start: 22
end: 93
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2119042
display id: annotation2119042
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119042/range2119042/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119042/range2119042/1
type: Range
persistent identity: range2119042
display id: range2119042
version: 1
direction: direction/reverse
top level: pSB1C3
start: 261
end: 875
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2119038
display id: annotation2119038
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119038/range2119038/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119038/range2119038/1
type: Range
persistent identity: range2119038
display id: range2119038
version: 1
direction: direction/forward
top level: pSB1C3
start: 1
end: 21
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2119040
display id: annotation2119040
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119040/range2119040/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119040/range2119040/1
type: Range
persistent identity: range2119040
display id: range2119040
version: 1
direction: direction/forward
top level: pSB1C3
start: 30
end: 64
orientation: inline

BBa_K2100082
type: ComponentDefinition
persistent identity: BBa K2100082
display id: BBa_K2100082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: miRNA Sensor Backbone
creator: Archis R. Bhandarkar
created: 2016-10-20T11:00:00Z
modified: 2016-10-21T10:40:20Z
discontinued: false
dominant: false
group u list: _2568_
m user id: 32435
owner id: 32435
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the common plasmid backbone for all the miRNA sensors used in our project. They contain both ampicillin and kanamycin resistance marker.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K2100082_sequence/1

pSB1AC3
type: ComponentDefinition
persistent identity: pSB1AC3
display id: pSB1AC3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Tom Knight
created: 2005-06-06T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _42_
m user id: 0
owner id: 6
owning group id: 42
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.
mutable provenance: pSB1A3 with added Cm resistance
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Prefix
sequence annotation: rep (pMB1) (rev)
sequence annotation: ORI
sequence annotation: deletion of two bases
sequence annotation: terminator stem loop
sequence annotation: end of terminator
sequence annotation: APr (rev)
sequence annotation: E. coli his operon terminator
sequence annotation: VR
sequence annotation: Cm R (rev)
sequence annotation: Silent g->a mutation found during sequencing
sequence annotation: Suffix
sequence annotation: VF2
sequence annotation: T0 terminator
sequence: https://synbiohub.org/public/igem/pSB1AC3_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation1779618
display id: annotation1779618
version: 1
top level: pSB1AC3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779618/range1779618/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779618/range1779618/1
type: Range
persistent identity: range1779618
display id: range1779618
version: 1
direction: direction/forward
top level: pSB1AC3
start: 3034
end: 3055
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1779628
display id: annotation1779628
version: 1
top level: pSB1AC3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779628/range1779628/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779628/range1779628/1
type: Range
persistent identity: range1779628
display id: range1779628
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 261
end: 875
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1779653
display id: annotation1779653
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779653/range1779653/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779653/range1779653/1
type: Range
persistent identity: range1779653
display id: range1779653
version: 1
direction: direction/forward
top level: pSB1AC3
start: 276
end: 276
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823815
display id: annotation1823815
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1823815/range1823815/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1823815/range1823815/1
type: Range
persistent identity: range1823815
display id: range1823815
version: 1
direction: direction/forward
top level: pSB1AC3
start: 34
end: 35
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1779720
display id: annotation1779720
version: 1
top level: pSB1AC3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779720/range1779720/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779720/range1779720/1
type: Range
persistent identity: range1779720
display id: range1779720
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 3005
end: 3024
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1823949
display id: annotation1823949
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1823949/range1823949/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1823949/range1823949/1
type: Range
persistent identity: range1823949
display id: range1823949
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 2992
end: 2995
orientation: inline

APr (rev)
type: SequenceAnnotation
persistent identity: annotation1779661
display id: annotation1779661
version: 1
top level: pSB1AC3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779661/range1779661/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779661/range1779661/1
type: Range
persistent identity: range1779661
display id: range1779661
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 1933
end: 2793
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1779665
display id: annotation1779665
version: 1
top level: pSB1AC3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779665/range1779665/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779665/range1779665/1
type: Range
persistent identity: range1779665
display id: range1779665
version: 1
direction: direction/forward
top level: pSB1AC3
start: 30
end: 64
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1823948
display id: annotation1823948
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1823948/range1823948/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1823948/range1823948/1
type: Range
persistent identity: range1823948
display id: range1823948
version: 1
direction: direction/forward
top level: pSB1AC3
start: 22
end: 93
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1779731
display id: annotation1779731
version: 1
top level: pSB1AC3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779731/range1779731/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779731/range1779731/1
type: Range
persistent identity: range1779731
display id: range1779731
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 157
end: 176
orientation: inline

Cm R (rev)
type: SequenceAnnotation
persistent identity: annotation1779657
display id: annotation1779657
version: 1
top level: pSB1AC3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779657/range1779657/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779657/range1779657/1
type: Range
persistent identity: range1779657
display id: range1779657
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 1163
end: 1821
orientation: inline

Silent g->a mutation found during sequencing
type: SequenceAnnotation
persistent identity: annotation1782173
display id: annotation1782173
version: 1
top level: pSB1AC3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1782173/range1782173/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1782173/range1782173/1
type: Range
persistent identity: range1782173
display id: range1782173
version: 1
direction: direction/forward
top level: pSB1AC3
start: 1237
end: 1237
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1779617
display id: annotation1779617
version: 1
top level: pSB1AC3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779617/range1779617/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779617/range1779617/1
type: Range
persistent identity: range1779617
display id: range1779617
version: 1
direction: direction/forward
top level: pSB1AC3
start: 1
end: 21
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1779730
display id: annotation1779730
version: 1
top level: pSB1AC3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779730/range1779730/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779730/range1779730/1
type: Range
persistent identity: range1779730
display id: range1779730
version: 1
direction: direction/forward
top level: pSB1AC3
start: 2916
end: 2935
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation1782172
display id: annotation1782172
version: 1
top level: pSB1AC3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1782172/range1782172/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1782172/range1782172/1
type: Range
persistent identity: range1782172
display id: range1782172
version: 1
direction: direction/forward
top level: pSB1AC3
start: 1042
end: 1147
orientation: inline

BBa_K797007
type: ComponentDefinition
persistent identity: BBa K797007
display id: BBa_K797007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter with 6 His coding region
creator: Tomohiro Nobeyama
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1053_
m user id: 0
owner id: 11588
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This improved T7 promoter has not only promoter region but also Histigine Tag sequence. This promoter let you correct and purify proteins by using His Tag antibody
mutable notes: This promoter has RBS region.
mutable provenance: This parts is synthesized artificially.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

BBa_K1480018
type: ComponentDefinition
persistent identity: BBa K1480018
display id: BBa_K1480018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB3C5 + AF and BF TALEN (K1480001) Target sites
creator: Roberto Alejandro Valderrama Alfaro
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1860_
m user id: 0
owner id: 19018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Backbone vector of pSB3C5 that contains two modifications with a TALEN target site after and before the origin of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains two TALEN target sites (AF+BF)
mutable notes: Modifications where inserted by PCR on both directions due to TALEN specificity.
mutable provenance: Derived from pSB3C5 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1480018_sequence/1

BBa_J72206
type: ComponentDefinition
persistent identity: BBa J72206
display id: BBa_J72206
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ampicillin resistant split R6K vector
creator: Josh Kittleson
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:08:29Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ampicillin resistant split R6K vector. Requires expression of pir to replicate. Referred to internally as pBjk2993.
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72206_sequence/1

BBa_J72202
type: ComponentDefinition
persistent identity: BBa J72202
display id: BBa_J72202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ampicillin resistant p15A vector
creator: Josh Kittleson
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:08:28Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ampicillin resistant p15A vector referred to internally as pBjo1601A
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72202_sequence/1

BBa_K1362093
type: ComponentDefinition
persistent identity: BBa K1362093
display id: BBa_K1362093
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1K30: High copy BioBrick cloning/expression backbone carrying Kan resitance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ORI
sequence annotation: end of terminator
sequence annotation: VR
sequence annotation: T7 promotor
sequence annotation: E. coli his operon terminator
sequence annotation: suffix
sequence annotation: rep(pMB1) (rev)
sequence annotation: terminator stem loop
sequence annotation: BBa_K1362092
sequence annotation: prefix
sequence annotation: lacO reg
sequence annotation: VF2
sequence annotation: repeat region
sequence annotation: Kanamycin resistance
sequence: https://synbiohub.org/public/igem/BBa_K1362093_sequence/1

ORI
type: SequenceAnnotation
persistent identity: annotation2405076
display id: annotation2405076
version: 1
top level: BBa_K1362093
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405076/range2405076/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405076/range2405076/1
type: Range
persistent identity: range2405076
display id: range2405076
version: 1
direction: direction/forward
top level: BBa_K1362093
start: 276
end: 276
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2405080
display id: annotation2405080
version: 1
top level: BBa_K1362093
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405080/range2405080/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405080/range2405080/1
type: Range
persistent identity: range2405080
display id: range2405080
version: 1
direction: direction/reverse
top level: BBa_K1362093
start: 2143
end: 2146
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2405074
display id: annotation2405074
version: 1
top level: BBa_K1362093
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405074/range2405074/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405074/range2405074/1
type: Range
persistent identity: range2405074
display id: range2405074
version: 1
direction: direction/reverse
top level: BBa_K1362093
start: 157
end: 176
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2405082
display id: annotation2405082
version: 1
top level: BBa_K1362093
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405082/range2405082/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405082/range2405082/1
type: Range
persistent identity: range2405082
display id: range2405082
version: 1
direction: direction/forward
top level: BBa_K1362093
start: 2188
end: 2206
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2405072
display id: annotation2405072
version: 1
top level: BBa_K1362093
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405072/range2405072/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405072/range2405072/1
type: Range
persistent identity: range2405072
display id: range2405072
version: 1
direction: direction/forward
top level: BBa_K1362093
start: 22
end: 93
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2405071
display id: annotation2405071
version: 1
top level: BBa_K1362093
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405071/range2405071/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405071/range2405071/1
type: Range
persistent identity: range2405071
display id: range2405071
version: 1
direction: direction/forward
top level: BBa_K1362093
start: 1
end: 21
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2405075
display id: annotation2405075
version: 1
top level: BBa_K1362093
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405075/range2405075/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405075/range2405075/1
type: Range
persistent identity: range2405075
display id: range2405075
version: 1
direction: direction/reverse
top level: BBa_K1362093
start: 261
end: 875
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2405073
display id: annotation2405073
version: 1
top level: BBa_K1362093
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405073/range2405073/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405073/range2405073/1
type: Range
persistent identity: range2405073
display id: range2405073
version: 1
direction: direction/forward
top level: BBa_K1362093
start: 30
end: 64
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2405081
display id: annotation2405081
version: 1
top level: BBa_K1362093
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405081/range2405081/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405081/range2405081/1
type: Range
persistent identity: range2405081
display id: range2405081
version: 1
direction: direction/reverse
top level: BBa_K1362093
start: 2156
end: 2175
orientation: inline

BBa_K1362092
type: SequenceAnnotation
persistent identity: annotation2405085
display id: annotation2405085
version: 1
top level: BBa_K1362093
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405085/range2405085/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405085/range2405085/1
type: Range
persistent identity: range2405085
display id: range2405085
version: 1
direction: direction/reverse
top level: BBa_K1362093
start: 1
end: 2121
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2405084
display id: annotation2405084
version: 1
top level: BBa_K1362093
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405084/range2405084/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405084/range2405084/1
type: Range
persistent identity: range2405084
display id: range2405084
version: 1
direction: direction/forward
top level: BBa_K1362093
start: 2234
end: 2255
orientation: inline

lacO reg
type: SequenceAnnotation
persistent identity: annotation2405083
display id: annotation2405083
version: 1
top level: BBa_K1362093
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405083/range2405083/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405083/range2405083/1
type: Range
persistent identity: range2405083
display id: range2405083
version: 1
direction: direction/forward
top level: BBa_K1362093
start: 2206
end: 2233
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2405079
display id: annotation2405079
version: 1
top level: BBa_K1362093
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405079/range2405079/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405079/range2405079/1
type: Range
persistent identity: range2405079
display id: range2405079
version: 1
direction: direction/forward
top level: BBa_K1362093
start: 2067
end: 2086
orientation: inline

repeat region
type: SequenceAnnotation
persistent identity: annotation2405077
display id: annotation2405077
version: 1
top level: BBa_K1362093
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405077/range2405077/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405077/range2405077/1
type: Range
persistent identity: range2405077
display id: range2405077
version: 1
direction: direction/reverse
top level: BBa_K1362093
start: 2017
end: 2060
orientation: inline

Kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation2405078
display id: annotation2405078
version: 1
top level: BBa_K1362093
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1362093/annotation2405078/range2405078/1

https://synbiohub.org/public/igem/BBa_K1362093/annotation2405078/range2405078/1
type: Range
persistent identity: range2405078
display id: range2405078
version: 1
direction: direction/reverse
top level: BBa_K1362093
start: 1097
end: 1912
orientation: inline

BBa_K1199009
type: ComponentDefinition
persistent identity: BBa K1199009
display id: BBa_K1199009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified pBAD backbone
creator: Yanpu Chen
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:09:39Z
discontinued: false
dominant: false
group u list: _1513_
m user id: 0
owner id: 16920
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: We used the backbone constructed our plasmid in
mutable notes: we modified it mutiple cloning site.
mutable provenance: ef
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1199009_sequence/1

BBa_K1138002
type: ComponentDefinition
persistent identity: BBa K1138002
display id: BBa_K1138002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAW50-linker-mcherry(+RBS) (ugaiGEM12)
creator: Rachit Jain
created: 2013-09-05T11:00:00Z
modified: 2015-05-08T01:09:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1450_
m user id: 0
owner id: 12767
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is similar to part BBa_K1138001 with the difference that an RBS sequence is inserted between the XbaI and NsiI restriction sites. This part can serve as a control while doing experiments utilizing part BBa_K1138001. This plasmid consists of the mcherry gene (encoding red fluorescence protein) inserted in the backbone of pAW50 vector. EcoRI-NotI-XbaI-RBS-NsiI restriction sites have been introduced followed by a linker sequence followed by the mcherry sequence. The use of these sites will allow users to introduce any desired gene of their choice to be fused with the linker which is already fused with the mcherry sequence. This way any protein can be qualitatively studied in Methanogens via red fluorescence. Note:- the users will have to introduce the RBS sequence and start codon, delete the terminator sequence of their gene while cloning.
mutable notes: Primers were designed in a way to introduce EcoRI-NotI-XbaI-RBS- NsiI sites before the linker.
mutable provenance: mcherry was obtained from Discosoma sp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: EcoRI
sequence annotation: promoter
sequence annotation: linker sequence
sequence annotation: mcherry
sequence annotation: NsiI
sequence annotation: RBS
sequence annotation: ampicillin
sequence annotation: puramycin
sequence annotation: NotI
sequence annotation: XbaI
sequence: https://synbiohub.org/public/igem/BBa_K1138002_sequence/1

EcoRI
type: SequenceAnnotation
persistent identity: annotation2335223
display id: annotation2335223
version: 1
top level: BBa_K1138002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138002/annotation2335223/range2335223/1

https://synbiohub.org/public/igem/BBa_K1138002/annotation2335223/range2335223/1
type: Range
persistent identity: range2335223
display id: range2335223
version: 1
direction: direction/forward
top level: BBa_K1138002
start: 205
end: 210
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2335221
display id: annotation2335221
version: 1
top level: BBa_K1138002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1138002/annotation2335221/range2335221/1

https://synbiohub.org/public/igem/BBa_K1138002/annotation2335221/range2335221/1
type: Range
persistent identity: range2335221
display id: range2335221
version: 1
direction: direction/forward
top level: BBa_K1138002
start: 18
end: 204
orientation: inline

linker sequence
type: SequenceAnnotation
persistent identity: annotation2335228
display id: annotation2335228
version: 1
top level: BBa_K1138002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138002/annotation2335228/range2335228/1

https://synbiohub.org/public/igem/BBa_K1138002/annotation2335228/range2335228/1
type: Range
persistent identity: range2335228
display id: range2335228
version: 1
direction: direction/forward
top level: BBa_K1138002
start: 239
end: 266
orientation: inline

mcherry
type: SequenceAnnotation
persistent identity: annotation2335229
display id: annotation2335229
version: 1
top level: BBa_K1138002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1138002/annotation2335229/range2335229/1

https://synbiohub.org/public/igem/BBa_K1138002/annotation2335229/range2335229/1
type: Range
persistent identity: range2335229
display id: range2335229
version: 1
direction: direction/forward
top level: BBa_K1138002
start: 267
end: 974
orientation: inline

NsiI
type: SequenceAnnotation
persistent identity: annotation2335227
display id: annotation2335227
version: 1
top level: BBa_K1138002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138002/annotation2335227/range2335227/1

https://synbiohub.org/public/igem/BBa_K1138002/annotation2335227/range2335227/1
type: Range
persistent identity: range2335227
display id: range2335227
version: 1
direction: direction/forward
top level: BBa_K1138002
start: 233
end: 238
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2335226
display id: annotation2335226
version: 1
top level: BBa_K1138002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1138002/annotation2335226/range2335226/1

https://synbiohub.org/public/igem/BBa_K1138002/annotation2335226/range2335226/1
type: Range
persistent identity: range2335226
display id: range2335226
version: 1
direction: direction/forward
top level: BBa_K1138002
start: 226
end: 231
orientation: inline

ampicillin
type: SequenceAnnotation
persistent identity: annotation2335231
display id: annotation2335231
version: 1
top level: BBa_K1138002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138002/annotation2335231/range2335231/1

https://synbiohub.org/public/igem/BBa_K1138002/annotation2335231/range2335231/1
type: Range
persistent identity: range2335231
display id: range2335231
version: 1
direction: direction/forward
top level: BBa_K1138002
start: 2436
end: 3296
orientation: inline

puramycin
type: SequenceAnnotation
persistent identity: annotation2335230
display id: annotation2335230
version: 1
top level: BBa_K1138002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138002/annotation2335230/range2335230/1

https://synbiohub.org/public/igem/BBa_K1138002/annotation2335230/range2335230/1
type: Range
persistent identity: range2335230
display id: range2335230
version: 1
direction: direction/forward
top level: BBa_K1138002
start: 1054
end: 1653
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation2335224
display id: annotation2335224
version: 1
top level: BBa_K1138002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138002/annotation2335224/range2335224/1

https://synbiohub.org/public/igem/BBa_K1138002/annotation2335224/range2335224/1
type: Range
persistent identity: range2335224
display id: range2335224
version: 1
direction: direction/forward
top level: BBa_K1138002
start: 211
end: 218
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2335225
display id: annotation2335225
version: 1
top level: BBa_K1138002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1138002/annotation2335225/range2335225/1

https://synbiohub.org/public/igem/BBa_K1138002/annotation2335225/range2335225/1
type: Range
persistent identity: range2335225
display id: range2335225
version: 1
direction: direction/forward
top level: BBa_K1138002
start: 220
end: 225
orientation: inline

pSB1C3 GB
type: ComponentDefinition
persistent identity: BBa K812050
display id: BBa_K812050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Golden brick version of pSB1C3 plasmid for Golden Gate and Golden Brick cloning
creator: Cyrille Pauthenier
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1069_
m user id: 0
owner id: 8998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is a modified version of pSB1C3 from the registry with the J4450 negative control. The biobrick sites are modified to incorporate a BsaI restriction site that enable to the use of golden gate cloning and golden brick cloning to assemble full contructs in a single shot of digestion/ligation. The complete protocol of the goldengate can be find in the original paper: Engler C, Gruetzner R, Kandzia R, Marillonnet S (2009) Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes. PLoS ONE 4(5): e5553. doi:10.1371/journal.pone.0005553 The Golden Brick assembly is an invention of the iGEM Evry 2012 Team. A complete description of the procedure can be find on our website: http://2012.igem.org/Team:Evry/GB
mutable notes: The negative control J4450 has been amplified with new goldenbrick extensions using PCR and re-inserted in pSB1C3 using EcoRI and PstI cloning.
mutable provenance: This part is a derivation of the pSB1C3 plasmid from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BbsI
sequence annotation: Terminator
sequence annotation: SpeI
sequence annotation: VR
sequence annotation: OH1
sequence annotation: VF2
sequence annotation: T0 terminator
sequence annotation: BsaI
sequence annotation: Golden Brick Back
sequence annotation: E. coli His Operon terminator
sequence annotation: XbaI
sequence annotation: EcoRI
sequence annotation: Cloramphenicol resistance CDS
sequence annotation: Bbsi
sequence annotation: OH5
sequence annotation: PstI
sequence annotation: pSB1C3 backbone
sequence annotation: pMB1 replication origin
sequence: https://synbiohub.org/public/igem/BBa_K812050_sequence/1

BbsI
type: SequenceAnnotation
persistent identity: annotation2187755
display id: annotation2187755
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187755/range2187755/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187755/range2187755/1
type: Range
persistent identity: range2187755
display id: range2187755
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 17
end: 22
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2187774
display id: annotation2187774
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187774/range2187774/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187774/range2187774/1
type: Range
persistent identity: range2187774
display id: range2187774
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 2030
end: 2049
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2187752
display id: annotation2187752
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187752/range2187752/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187752/range2187752/1
type: Range
persistent identity: range2187752
display id: range2187752
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 1
end: 6
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2187763
display id: annotation2187763
version: 1
top level: pSB1C3 GB
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187763/range2187763/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187763/range2187763/1
type: Range
persistent identity: range2187763
display id: range2187763
version: 1
direction: direction/reverse
top level: pSB1C3 GB
start: 165
end: 184
orientation: inline

OH1
type: SequenceAnnotation
persistent identity: annotation2187962
display id: annotation2187962
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187962/range2187962/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187962/range2187962/1
type: Range
persistent identity: range2187962
display id: range2187962
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 2068
end: 2071
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2187768
display id: annotation2187768
version: 1
top level: pSB1C3 GB
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187768/range2187768/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187768/range2187768/1
type: Range
persistent identity: range2187768
display id: range2187768
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 1941
end: 1960
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2187766
display id: annotation2187766
version: 1
top level: pSB1C3 GB
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187766/range2187766/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187766/range2187766/1
type: Range
persistent identity: range2187766
display id: range2187766
version: 1
direction: direction/reverse
top level: pSB1C3 GB
start: 1050
end: 1155
orientation: inline

BsaI
type: SequenceAnnotation
persistent identity: annotation2187963
display id: annotation2187963
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187963/range2187963/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187963/range2187963/1
type: Range
persistent identity: range2187963
display id: range2187963
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 2073
end: 2078
orientation: inline

Golden Brick Back
type: SequenceAnnotation
persistent identity: annotation2187749
display id: annotation2187749
version: 1
top level: pSB1C3 GB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187749/range2187749/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187749/range2187749/1
type: Range
persistent identity: range2187749
display id: range2187749
version: 1
direction: direction/reverse
top level: pSB1C3 GB
start: 1
end: 27
orientation: inline

E. coli His Operon terminator
type: SequenceAnnotation
persistent identity: annotation2187762
display id: annotation2187762
version: 1
top level: pSB1C3 GB
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187762/range2187762/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187762/range2187762/1
type: Range
persistent identity: range2187762
display id: range2187762
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 29
end: 101
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2188002
display id: annotation2188002
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2188002/range2188002/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2188002/range2188002/1
type: Range
persistent identity: range2188002
display id: range2188002
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 2079
end: 2084
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2187897
display id: annotation2187897
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187897/range2187897/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187897/range2187897/1
type: Range
persistent identity: range2187897
display id: range2187897
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 2058
end: 2062
orientation: inline

Cloramphenicol resistance CDS
type: SequenceAnnotation
persistent identity: annotation2187767
display id: annotation2187767
version: 1
top level: pSB1C3 GB
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187767/range2187767/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187767/range2187767/1
type: Range
persistent identity: range2187767
display id: range2187767
version: 1
direction: direction/reverse
top level: pSB1C3 GB
start: 1168
end: 1827
orientation: inline

Bbsi
type: SequenceAnnotation
persistent identity: annotation2187930
display id: annotation2187930
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187930/range2187930/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187930/range2187930/1
type: Range
persistent identity: range2187930
display id: range2187930
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 2063
end: 2068
orientation: inline

OH5
type: SequenceAnnotation
persistent identity: annotation2187757
display id: annotation2187757
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187757/range2187757/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187757/range2187757/1
type: Range
persistent identity: range2187757
display id: range2187757
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 14
end: 17
orientation: inline

Golden Brick Back
type: SequenceAnnotation
persistent identity: annotation2187792
display id: annotation2187792
version: 1
top level: pSB1C3 GB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187792/range2187792/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187792/range2187792/1
type: Range
persistent identity: range2187792
display id: range2187792
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 2058
end: 2084
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2187754
display id: annotation2187754
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187754/range2187754/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187754/range2187754/1
type: Range
persistent identity: range2187754
display id: range2187754
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 23
end: 27
orientation: inline

BsaI
type: SequenceAnnotation
persistent identity: annotation2187753
display id: annotation2187753
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187753/range2187753/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187753/range2187753/1
type: Range
persistent identity: range2187753
display id: range2187753
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 7
end: 12
orientation: inline

pSB1C3 backbone
type: SequenceAnnotation
persistent identity: annotation2187758
display id: annotation2187758
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187758/range2187758/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187758/range2187758/1
type: Range
persistent identity: range2187758
display id: range2187758
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 28
end: 2057
orientation: inline

pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation2187764
display id: annotation2187764
version: 1
top level: pSB1C3 GB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K812050/annotation2187764/range2187764/1

https://synbiohub.org/public/igem/BBa_K812050/annotation2187764/range2187764/1
type: Range
persistent identity: range2187764
display id: range2187764
version: 1
direction: direction/forward
top level: pSB1C3 GB
start: 269
end: 883
orientation: inline

BBa_K823055
type: ComponentDefinition
persistent identity: BBa K823055
display id: BBa_K823055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3F-Vector pSB1C3 with RFP-cassette in Freiburg Standard
creator: Jara Radeck
created: 2013-10-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To allow the possibility to save some nucleotides of primer-overhangs, you can use this vector. The two AgeI-restriction sites from the original RFP-cassette have been removed. The cassette is flanked by the modified Freiburg standard (http://2012.igem.org/wiki/images/b/b9/LMU-Munich_2012_Our_Freiburg_standard_FusionPrefix.pdf) that includes an optimal ribosome binding site between XbaI and NgoMIV restriction sites.
mutable notes: Two AgeI-restriction sites have been removed by two subsequent quikchange site-directed mutagnesis reactions. The RFP-cassette was PCR-amplified with the overhangs: prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
mutable provenance: pSB1C3; the RFP-part is originally from Discosoma sp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ori (pMB1)
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K823055_sequence/1

ori (pMB1)
type: SequenceAnnotation
persistent identity: annotation2371772
display id: annotation2371772
version: 1
top level: BBa_K823055
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823055/annotation2371772/range2371772/1

https://synbiohub.org/public/igem/BBa_K823055/annotation2371772/range2371772/1
type: Range
persistent identity: range2371772
display id: range2371772
version: 1
direction: direction/forward
top level: BBa_K823055
start: 260
end: 874
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2371771
display id: annotation2371771
version: 1
top level: BBa_K823055
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823055/annotation2371771/range2371771/1

https://synbiohub.org/public/igem/BBa_K823055/annotation2371771/range2371771/1
type: Range
persistent identity: range2371771
display id: range2371771
version: 1
direction: direction/reverse
top level: BBa_K823055
start: 1169
end: 1828
orientation: inline

BBa_K590012
type: ComponentDefinition
persistent identity: BBa K590012
display id: BBa_K590012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGA4C5, Gibson assembly plasmid (bglBrick) with pLac-GFP insert
creator: Michael Brasino, Alicia Wong, Rashmi Ravichandran
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Backbone that is Gibson reaction friendly (Bgl Brick)
mutable notes: sequence confirmed
mutable provenance: Made from Gibson reaction
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BgIBrK Suffix, XhoI
sequence annotation: terminator stem loop
sequence annotation: Vr
sequence annotation: 3K3 Pre-Prefix
sequence annotation: pGA Prefix
sequence annotation: pGA Suffix
sequence: https://synbiohub.org/public/igem/BBa_K590012_sequence/1

BgIBrK Suffix, XhoI
type: SequenceAnnotation
persistent identity: annotation2145540
display id: annotation2145540
version: 1
top level: BBa_K590012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K590012/annotation2145540/range2145540/1

https://synbiohub.org/public/igem/BBa_K590012/annotation2145540/range2145540/1
type: Range
persistent identity: range2145540
display id: range2145540
version: 1
direction: direction/forward
top level: BBa_K590012
start: 16
end: 21
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2145541
display id: annotation2145541
version: 1
top level: BBa_K590012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K590012/annotation2145541/range2145541/1

https://synbiohub.org/public/igem/BBa_K590012/annotation2145541/range2145541/1
type: Range
persistent identity: range2145541
display id: range2145541
version: 1
direction: direction/forward
top level: BBa_K590012
start: 56
end: 88
orientation: inline

Vr
type: SequenceAnnotation
persistent identity: annotation2145542
display id: annotation2145542
version: 1
top level: BBa_K590012
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K590012/annotation2145542/range2145542/1

https://synbiohub.org/public/igem/BBa_K590012/annotation2145542/range2145542/1
type: Range
persistent identity: range2145542
display id: range2145542
version: 1
direction: direction/reverse
top level: BBa_K590012
start: 116
end: 135
orientation: inline

3K3 Pre-Prefix
type: SequenceAnnotation
persistent identity: annotation2145543
display id: annotation2145543
version: 1
top level: BBa_K590012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K590012/annotation2145543/range2145543/1

https://synbiohub.org/public/igem/BBa_K590012/annotation2145543/range2145543/1
type: Range
persistent identity: range2145543
display id: range2145543
version: 1
direction: direction/reverse
top level: BBa_K590012
start: 143
end: 178
orientation: inline

pGA Prefix
type: SequenceAnnotation
persistent identity: annotation2145544
display id: annotation2145544
version: 1
top level: BBa_K590012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K590012/annotation2145544/range2145544/1

https://synbiohub.org/public/igem/BBa_K590012/annotation2145544/range2145544/1
type: Range
persistent identity: range2145544
display id: range2145544
version: 1
direction: direction/reverse
top level: BBa_K590012
start: 3200
end: 3220
orientation: inline

pGA Suffix
type: SequenceAnnotation
persistent identity: annotation2145539
display id: annotation2145539
version: 1
top level: BBa_K590012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K590012/annotation2145539/range2145539/1

https://synbiohub.org/public/igem/BBa_K590012/annotation2145539/range2145539/1
type: Range
persistent identity: range2145539
display id: range2145539
version: 1
direction: direction/forward
top level: BBa_K590012
start: 1
end: 15
orientation: inline

pSB1A7
type: ComponentDefinition
persistent identity: pSB1A7
display id: pSB1A7
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transcriptionally insulated high copy BioBrick plasmid
creator: Karmella Haynes
created: 2006-10-18T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pSB1A4 was constructed by adding double terminators ([BBa_B0015]) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. This insulator blocks read-through transcription into BioBrick parts/ devices.
mutable notes: The double terminators were assembled from small smaller overlapping ssDNA oligos. The outer-most EcoRI and PstI sites were mutated while the inner-most BioBrick cut sites were restored.
mutable provenance: Based upon pSB1A3; Includes two copies of [BBa_B0015] assembled from DNA oligos
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR
sequence annotation: c > g
sequence annotation: prefix
sequence annotation: E. coli his operon terminator
sequence annotation: VF2
sequence annotation: stem_loop
sequence annotation: ampR (rev)
sequence annotation: mutated EcoRI
sequence annotation: mutated PstI
sequence annotation: end of terminator
sequence annotation: rep(pMB1) (rev)
sequence annotation: B0015 (rev)
sequence annotation: B0015
sequence annotation: suffix
sequence: https://synbiohub.org/public/igem/pSB1A7_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1903651
display id: annotation1903651
version: 1
top level: pSB1A7
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903651/range1903651/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903651/range1903651/1
type: Range
persistent identity: range1903651
display id: range1903651
version: 1
direction: direction/reverse
top level: pSB1A7
start: 294
end: 313
orientation: inline

c > g
type: SequenceAnnotation
persistent identity: annotation1903647
display id: annotation1903647
version: 1
top level: pSB1A7
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903647/range1903647/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903647/range1903647/1
type: Range
persistent identity: range1903647
display id: range1903647
version: 1
direction: direction/forward
top level: pSB1A7
start: 153
end: 153
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1903661
display id: annotation1903661
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903661/range1903661/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903661/range1903661/1
type: Range
persistent identity: range1903661
display id: range1903661
version: 1
direction: direction/forward
top level: pSB1A7
start: 2411
end: 2431
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1903649
display id: annotation1903649
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903649/range1903649/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903649/range1903649/1
type: Range
persistent identity: range1903649
display id: range1903649
version: 1
direction: direction/forward
top level: pSB1A7
start: 159
end: 229
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1903643
display id: annotation1903643
version: 1
top level: pSB1A7
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903643/range1903643/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903643/range1903643/1
type: Range
persistent identity: range1903643
display id: range1903643
version: 1
direction: direction/forward
top level: pSB1A7
start: 2155
end: 2174
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903645
display id: annotation1903645
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903645/range1903645/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903645/range1903645/1
type: Range
persistent identity: range1903645
display id: range1903645
version: 1
direction: direction/reverse
top level: pSB1A7
start: 38
end: 57
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903650
display id: annotation1903650
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903650/range1903650/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903650/range1903650/1
type: Range
persistent identity: range1903650
display id: range1903650
version: 1
direction: direction/forward
top level: pSB1A7
start: 167
end: 201
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation1903653
display id: annotation1903653
version: 1
top level: pSB1A7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903653/range1903653/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903653/range1903653/1
type: Range
persistent identity: range1903653
display id: range1903653
version: 1
direction: direction/reverse
top level: pSB1A7
start: 1172
end: 2032
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903646
display id: annotation1903646
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903646/range1903646/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903646/range1903646/1
type: Range
persistent identity: range1903646
display id: range1903646
version: 1
direction: direction/reverse
top level: pSB1A7
start: 98
end: 141
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903659
display id: annotation1903659
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903659/range1903659/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903659/range1903659/1
type: Range
persistent identity: range1903659
display id: range1903659
version: 1
direction: direction/forward
top level: pSB1A7
start: 2290
end: 2333
orientation: inline

mutated EcoRI
type: SequenceAnnotation
persistent identity: annotation1903656
display id: annotation1903656
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903656/range1903656/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903656/range1903656/1
type: Range
persistent identity: range1903656
display id: range1903656
version: 1
direction: direction/forward
top level: pSB1A7
start: 2273
end: 2278
orientation: inline

mutated PstI
type: SequenceAnnotation
persistent identity: annotation1903648
display id: annotation1903648
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903648/range1903648/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903648/range1903648/1
type: Range
persistent identity: range1903648
display id: range1903648
version: 1
direction: direction/forward
top level: pSB1A7
start: 153
end: 158
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903655
display id: annotation1903655
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903655/range1903655/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903655/range1903655/1
type: Range
persistent identity: range1903655
display id: range1903655
version: 1
direction: direction/forward
top level: pSB1A7
start: 2244
end: 2263
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1903654
display id: annotation1903654
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903654/range1903654/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903654/range1903654/1
type: Range
persistent identity: range1903654
display id: range1903654
version: 1
direction: direction/forward
top level: pSB1A7
start: 2231
end: 2234
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1903652
display id: annotation1903652
version: 1
top level: pSB1A7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903652/range1903652/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903652/range1903652/1
type: Range
persistent identity: range1903652
display id: range1903652
version: 1
direction: direction/reverse
top level: pSB1A7
start: 398
end: 1012
orientation: inline

c > g
type: SequenceAnnotation
persistent identity: annotation1903657
display id: annotation1903657
version: 1
top level: pSB1A7
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903657/range1903657/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903657/range1903657/1
type: Range
persistent identity: range1903657
display id: range1903657
version: 1
direction: direction/forward
top level: pSB1A7
start: 2278
end: 2278
orientation: inline

B0015 (rev)
type: SequenceAnnotation
persistent identity: annotation1903644
display id: annotation1903644
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903644/range1903644/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903644/range1903644/1
type: Range
persistent identity: range1903644
display id: range1903644
version: 1
direction: direction/reverse
top level: pSB1A7
start: 24
end: 152
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation1903658
display id: annotation1903658
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903658/range1903658/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903658/range1903658/1
type: Range
persistent identity: range1903658
display id: range1903658
version: 1
direction: direction/forward
top level: pSB1A7
start: 2279
end: 2407
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1903642
display id: annotation1903642
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903642/range1903642/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903642/range1903642/1
type: Range
persistent identity: range1903642
display id: range1903642
version: 1
direction: direction/forward
top level: pSB1A7
start: 1
end: 20
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903660
display id: annotation1903660
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903660/range1903660/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903660/range1903660/1
type: Range
persistent identity: range1903660
display id: range1903660
version: 1
direction: direction/forward
top level: pSB1A7
start: 2374
end: 2393
orientation: inline

BBa_K1365301
type: ComponentDefinition
persistent identity: BBa K1365301
display id: BBa_K1365301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pIL253-mRFP Biobrick Vector for L.lactis
creator: Lisa Hielkema
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:07Z
discontinued: false
dominant: false
group u list: _1741_
m user id: 0
owner id: 20097
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This plasmid can be used to transform biobricks in L. lactis. Moreover it shuttles between L. lactis and B. subtilis. It contains mRFP Biobrick Prefix and Sufix. And it has an erythromycin resistance.
mutable notes: Cut out the multiple cloning sites of pIL253 also removed illegal restriction sites of EcoRI and XbaI
mutable provenance: Yes, Derived from pIL253. Provided by molGen at the university of Groningen.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: CAP binding site
sequence annotation: J04450 (mRFP Construct)
sequence annotation: G00100 (VF2)
sequence annotation: prefix
sequence annotation: suffix
sequence annotation: -10
sequence annotation: start
sequence annotation: ORF3
sequence annotation: E1010 (mRFP)
sequence annotation: B0010
sequence annotation: B0012
sequence annotation: Erythromycin resistance
sequence annotation: Origin of Replication
sequence annotation: B0034 (RBS)
sequence annotation: LacI binding site
sequence annotation: stop
sequence annotation: end of LacI coding region (inactive)
sequence annotation: B0015
sequence annotation: -35
sequence annotation: R0010 (LacI reg. promoter)
sequence annotation: G00101 (VR)
sequence: https://synbiohub.org/public/igem/BBa_K1365301_sequence/1

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2420363
display id: annotation2420363
version: 1
top level: BBa_K1365301
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420363/range2420363/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420363/range2420363/1
type: Range
persistent identity: range2420363
display id: range2420363
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 234
end: 271
orientation: inline

J04450 (mRFP Construct)
type: SequenceAnnotation
persistent identity: annotation2420329
display id: annotation2420329
version: 1
top level: BBa_K1365301
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420329/range2420329/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420329/range2420329/1
type: Range
persistent identity: range2420329
display id: range2420329
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 146
end: 1214
orientation: inline

G00100 (VF2)
type: SequenceAnnotation
persistent identity: annotation2420328
display id: annotation2420328
version: 1
top level: BBa_K1365301
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420328/range2420328/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420328/range2420328/1
type: Range
persistent identity: range2420328
display id: range2420328
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 6
end: 25
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2420789
display id: annotation2420789
version: 1
top level: BBa_K1365301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420789/range2420789/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420789/range2420789/1
type: Range
persistent identity: range2420789
display id: range2420789
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 124
end: 145
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2420790
display id: annotation2420790
version: 1
top level: BBa_K1365301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420790/range2420790/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420790/range2420790/1
type: Range
persistent identity: range2420790
display id: range2420790
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 1215
end: 1235
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2420370
display id: annotation2420370
version: 1
top level: BBa_K1365301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420370/range2420370/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420370/range2420370/1
type: Range
persistent identity: range2420370
display id: range2420370
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 306
end: 311
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2420791
display id: annotation2420791
version: 1
top level: BBa_K1365301
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420791/range2420791/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420791/range2420791/1
type: Range
persistent identity: range2420791
display id: range2420791
version: 1
direction: direction/reverse
top level: BBa_K1365301
start: 5696
end: 5696
orientation: inline

ORF3
type: SequenceAnnotation
persistent identity: annotation2420331
display id: annotation2420331
version: 1
top level: BBa_K1365301
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420331/range2420331/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420331/range2420331/1
type: Range
persistent identity: range2420331
display id: range2420331
version: 1
direction: direction/reverse
top level: BBa_K1365301
start: 4368
end: 4838
orientation: inline

E1010 (mRFP)
type: SequenceAnnotation
persistent identity: annotation2420378
display id: annotation2420378
version: 1
top level: BBa_K1365301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420378/range2420378/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420378/range2420378/1
type: Range
persistent identity: range2420378
display id: range2420378
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 372
end: 1046
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation2420436
display id: annotation2420436
version: 1
top level: BBa_K1365301
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420436/range2420436/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420436/range2420436/1
type: Range
persistent identity: range2420436
display id: range2420436
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 1087
end: 1143
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation2420437
display id: annotation2420437
version: 1
top level: BBa_K1365301
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420437/range2420437/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420437/range2420437/1
type: Range
persistent identity: range2420437
display id: range2420437
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 1181
end: 1200
orientation: inline

Erythromycin resistance
type: SequenceAnnotation
persistent identity: annotation2420332
display id: annotation2420332
version: 1
top level: BBa_K1365301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420332/range2420332/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420332/range2420332/1
type: Range
persistent identity: range2420332
display id: range2420332
version: 1
direction: direction/reverse
top level: BBa_K1365301
start: 4959
end: 5696
orientation: inline

Origin of Replication
type: SequenceAnnotation
persistent identity: annotation2420330
display id: annotation2420330
version: 1
top level: BBa_K1365301
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420330/range2420330/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420330/range2420330/1
type: Range
persistent identity: range2420330
display id: range2420330
version: 1
direction: direction/reverse
top level: BBa_K1365301
start: 1885
end: 3375
orientation: inline

B0034 (RBS)
type: SequenceAnnotation
persistent identity: annotation2420376
display id: annotation2420376
version: 1
top level: BBa_K1365301
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420376/range2420376/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420376/range2420376/1
type: Range
persistent identity: range2420376
display id: range2420376
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 354
end: 365
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2420374
display id: annotation2420374
version: 1
top level: BBa_K1365301
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420374/range2420374/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420374/range2420374/1
type: Range
persistent identity: range2420374
display id: range2420374
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 311
end: 345
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2420380
display id: annotation2420380
version: 1
top level: BBa_K1365301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420380/range2420380/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420380/range2420380/1
type: Range
persistent identity: range2420380
display id: range2420380
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 1047
end: 1047
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2420361
display id: annotation2420361
version: 1
top level: BBa_K1365301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420361/range2420361/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420361/range2420361/1
type: Range
persistent identity: range2420361
display id: range2420361
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 146
end: 233
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2420377
display id: annotation2420377
version: 1
top level: BBa_K1365301
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420377/range2420377/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420377/range2420377/1
type: Range
persistent identity: range2420377
display id: range2420377
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 372
end: 372
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2420375
display id: annotation2420375
version: 1
top level: BBa_K1365301
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420375/range2420375/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420375/range2420375/1
type: Range
persistent identity: range2420375
display id: range2420375
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 318
end: 318
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2420379
display id: annotation2420379
version: 1
top level: BBa_K1365301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420379/range2420379/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420379/range2420379/1
type: Range
persistent identity: range2420379
display id: range2420379
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 1050
end: 1050
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2420435
display id: annotation2420435
version: 1
top level: BBa_K1365301
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420435/range2420435/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420435/range2420435/1
type: Range
persistent identity: range2420435
display id: range2420435
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 1086
end: 1214
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2420366
display id: annotation2420366
version: 1
top level: BBa_K1365301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420366/range2420366/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420366/range2420366/1
type: Range
persistent identity: range2420366
display id: range2420366
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 282
end: 287
orientation: inline

R0010 (LacI reg. promoter)
type: SequenceAnnotation
persistent identity: annotation2420354
display id: annotation2420354
version: 1
top level: BBa_K1365301
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420354/range2420354/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420354/range2420354/1
type: Range
persistent identity: range2420354
display id: range2420354
version: 1
direction: direction/forward
top level: BBa_K1365301
start: 146
end: 345
orientation: inline

G00101 (VR)
type: SequenceAnnotation
persistent identity: annotation2420327
display id: annotation2420327
version: 1
top level: BBa_K1365301
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1365301/annotation2420327/range2420327/1

https://synbiohub.org/public/igem/BBa_K1365301/annotation2420327/range2420327/1
type: Range
persistent identity: range2420327
display id: range2420327
version: 1
direction: direction/reverse
top level: BBa_K1365301
start: 1369
end: 1388
orientation: inline

BBa_K1677200
type: ComponentDefinition
persistent identity: BBa K1677200
display id: BBa_K1677200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbA2-PHLS
creator: Mackenzie Labine-Romain
created: 2015-08-19T11:00:00Z
modified: 2015-09-14T09:34:32Z
discontinued: true
dominant: false
experience: experience/None
group u list: _2095_
m user id: 25019
owner id: 25019
owning group id: 9
status: status/Deleted
bookmark: false
mutable description: This integrative plasmid facilitates transformation of Synechocystis PCC6803 and similar species. It inserts gene of choice into the Synechocystis β-phellandrene synthase gene by homologous recombination.
mutable notes: Primers containing biobrick prefix and suffix as well and other described parts were used to make this backbone RFC10 compatible.
mutable provenance: Plasmid was purchased from open source plasmid repository Addgene.com. It was modified to be biobrick compatible. Formighieri, C., & Melis, A. (2014). Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta, 240(2), 309-324.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

pSB1AK3
type: ComponentDefinition
persistent identity: pSB1AK3
display id: pSB1AK3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Registry
created: 2004-07-27T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_48_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR
sequence annotation: E. coli his terminator
sequence annotation: deletion of two bases
sequence annotation: kanR (rev)
sequence annotation: terminator stem loop
sequence annotation: ORI
sequence annotation: Suffix
sequence annotation: VF2
sequence annotation: rep (pMB1) (rev)
sequence annotation: Prefix
sequence annotation: ampR (rev)
sequence annotation: end of terminator
sequence: https://synbiohub.org/public/igem/pSB1AK3_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1780436
display id: annotation1780436
version: 1
top level: pSB1AK3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780436/range1780436/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780436/range1780436/1
type: Range
persistent identity: range1780436
display id: range1780436
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 157
end: 176
orientation: inline

E. coli his terminator
type: SequenceAnnotation
persistent identity: annotation1823992
display id: annotation1823992
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1823992/range1823992/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1823992/range1823992/1
type: Range
persistent identity: range1823992
display id: range1823992
version: 1
direction: direction/forward
top level: pSB1AK3
start: 22
end: 93
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823805
display id: annotation1823805
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1823805/range1823805/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1823805/range1823805/1
type: Range
persistent identity: range1823805
display id: range1823805
version: 1
direction: direction/forward
top level: pSB1AK3
start: 34
end: 35
orientation: inline

kanR (rev)
type: SequenceAnnotation
persistent identity: annotation1780430
display id: annotation1780430
version: 1
top level: pSB1AK3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780430/range1780430/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780430/range1780430/1
type: Range
persistent identity: range1780430
display id: range1780430
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 1097
end: 1912
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780434
display id: annotation1780434
version: 1
top level: pSB1AK3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780434/range1780434/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780434/range1780434/1
type: Range
persistent identity: range1780434
display id: range1780434
version: 1
direction: direction/forward
top level: pSB1AK3
start: 30
end: 64
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1780432
display id: annotation1780432
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780432/range1780432/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780432/range1780432/1
type: Range
persistent identity: range1780432
display id: range1780432
version: 1
direction: direction/forward
top level: pSB1AK3
start: 276
end: 276
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1780400
display id: annotation1780400
version: 1
top level: pSB1AK3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780400/range1780400/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780400/range1780400/1
type: Range
persistent identity: range1780400
display id: range1780400
version: 1
direction: direction/forward
top level: pSB1AK3
start: 1
end: 21
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1780435
display id: annotation1780435
version: 1
top level: pSB1AK3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780435/range1780435/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780435/range1780435/1
type: Range
persistent identity: range1780435
display id: range1780435
version: 1
direction: direction/forward
top level: pSB1AK3
start: 3050
end: 3069
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1780429
display id: annotation1780429
version: 1
top level: pSB1AK3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780429/range1780429/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780429/range1780429/1
type: Range
persistent identity: range1780429
display id: range1780429
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 261
end: 875
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1780401
display id: annotation1780401
version: 1
top level: pSB1AK3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780401/range1780401/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780401/range1780401/1
type: Range
persistent identity: range1780401
display id: range1780401
version: 1
direction: direction/forward
top level: pSB1AK3
start: 3168
end: 3189
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation1780431
display id: annotation1780431
version: 1
top level: pSB1AK3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780431/range1780431/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780431/range1780431/1
type: Range
persistent identity: range1780431
display id: range1780431
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 2067
end: 2927
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1823993
display id: annotation1823993
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1823993/range1823993/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1823993/range1823993/1
type: Range
persistent identity: range1823993
display id: range1823993
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 3126
end: 3129
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780433
display id: annotation1780433
version: 1
top level: pSB1AK3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780433/range1780433/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780433/range1780433/1
type: Range
persistent identity: range1780433
display id: range1780433
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 3139
end: 3158
orientation: inline

BBa_J73998
type: ComponentDefinition
persistent identity: BBa J73998
display id: BBa_J73998
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plasmid vector for Rhodobacter sphaeroides
creator: Junling Huo
created: 2009-12-09T12:00:00Z
modified: 2015-05-08T01:08:29Z
discontinued: false
dominant: true
group u list: _303_
m user id: 0
owner id: 3154
owning group id: 303
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This vector is designed for Rhodobacter sphaeroides. You can transform it into Rhodoabcter spaherodies through conjugation.
mutable notes: To make it more flexible and easier to control, lacZ promoter and reporter have been removed.
mutable provenance: This vector backbone is based on pMG170/pMG171. lac promoter and reporter have been removed from the original vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J73998_sequence/1

BBa_K1480010
type: ComponentDefinition
persistent identity: BBa K1480010
display id: BBa_K1480010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3 + AF TALEN (K1480001) Target site
creator: Roberto Alejandro Valderrama Alfaro
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1860_
m user id: 0
owner id: 19018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Backbone vector of pSB1C3 that contains a modification with a TALEN target site after the origin of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains a single TALEN target site (AF).
mutable notes: Modifications where inserted by PCR on both directions due to TALEN specificity.
mutable provenance: Derived from pSB1C3 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1480010_sequence/1

BBa_K590011
type: ComponentDefinition
persistent identity: BBa K590011
display id: BBa_K590011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGA1C3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert
creator: Michael Brasino, Alicia Wong, Rashmi Ravichandran
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Backbone that is Gibson reaction friendly (Bgl Brick)
mutable notes: sequence confirmed
mutable provenance: Made from Gibson reaction
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pGA Prefix
sequence annotation: pGA Suffix
sequence annotation: pSB3K3 Post-Suffix
sequence annotation: Vr
sequence annotation: XbaI/SpeI Scar
sequence annotation: 3K3 Pre-prefix
sequence annotation: Vf2
sequence: https://synbiohub.org/public/igem/BBa_K590011_sequence/1

pGA Prefix
type: SequenceAnnotation
persistent identity: annotation2145313
display id: annotation2145313
version: 1
top level: BBa_K590011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K590011/annotation2145313/range2145313/1

https://synbiohub.org/public/igem/BBa_K590011/annotation2145313/range2145313/1
type: Range
persistent identity: range2145313
display id: range2145313
version: 1
direction: direction/reverse
top level: BBa_K590011
start: 2051
end: 2071
orientation: inline

pGA Suffix
type: SequenceAnnotation
persistent identity: annotation2145254
display id: annotation2145254
version: 1
top level: BBa_K590011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K590011/annotation2145254/range2145254/1

https://synbiohub.org/public/igem/BBa_K590011/annotation2145254/range2145254/1
type: Range
persistent identity: range2145254
display id: range2145254
version: 1
direction: direction/forward
top level: BBa_K590011
start: 1
end: 21
orientation: inline

pSB3K3 Post-Suffix
type: SequenceAnnotation
persistent identity: annotation2145309
display id: annotation2145309
version: 1
top level: BBa_K590011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K590011/annotation2145309/range2145309/1

https://synbiohub.org/public/igem/BBa_K590011/annotation2145309/range2145309/1
type: Range
persistent identity: range2145309
display id: range2145309
version: 1
direction: direction/forward
top level: BBa_K590011
start: 22
end: 57
orientation: inline

Vr
type: SequenceAnnotation
persistent identity: annotation2145308
display id: annotation2145308
version: 1
top level: BBa_K590011
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K590011/annotation2145308/range2145308/1

https://synbiohub.org/public/igem/BBa_K590011/annotation2145308/range2145308/1
type: Range
persistent identity: range2145308
display id: range2145308
version: 1
direction: direction/reverse
top level: BBa_K590011
start: 157
end: 176
orientation: inline

XbaI/SpeI Scar
type: SequenceAnnotation
persistent identity: annotation2145310
display id: annotation2145310
version: 1
top level: BBa_K590011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K590011/annotation2145310/range2145310/1

https://synbiohub.org/public/igem/BBa_K590011/annotation2145310/range2145310/1
type: Range
persistent identity: range2145310
display id: range2145310
version: 1
direction: direction/forward
top level: BBa_K590011
start: 709
end: 715
orientation: inline

3K3 Pre-prefix
type: SequenceAnnotation
persistent identity: annotation2145312
display id: annotation2145312
version: 1
top level: BBa_K590011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K590011/annotation2145312/range2145312/1

https://synbiohub.org/public/igem/BBa_K590011/annotation2145312/range2145312/1
type: Range
persistent identity: range2145312
display id: range2145312
version: 1
direction: direction/reverse
top level: BBa_K590011
start: 2016
end: 2050
orientation: inline

Vf2
type: SequenceAnnotation
persistent identity: annotation2145311
display id: annotation2145311
version: 1
top level: BBa_K590011
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K590011/annotation2145311/range2145311/1

https://synbiohub.org/public/igem/BBa_K590011/annotation2145311/range2145311/1
type: Range
persistent identity: range2145311
display id: range2145311
version: 1
direction: direction/forward
top level: BBa_K590011
start: 1932
end: 1952
orientation: inline

BBa_J119045
type: ComponentDefinition
persistent identity: BBa J119045
display id: BBa_J119045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C8
creator: Todd Eckdahl and A. Malcolm Campbell
created: 2012-01-19T12:00:00Z
modified: 2015-08-31T04:08:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Insulated vector based on pSB1C3. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix.
mutable notes: Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.
mutable provenance: de novo DNA synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Chloramphenicol resistance
sequence annotation: stuffer
sequence annotation: B0025 terminator
sequence annotation: B0010 terminator
sequence annotation: pMB1 replication origin
sequence annotation: VR primer
sequence annotation: Suffix
sequence annotation: VF2
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_J119045_sequence/1

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2169682
display id: annotation2169682
version: 1
top level: BBa_J119045
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J119045/annotation2169682/range2169682/1

https://synbiohub.org/public/igem/BBa_J119045/annotation2169682/range2169682/1
type: Range
persistent identity: range2169682
display id: range2169682
version: 1
direction: direction/reverse
top level: BBa_J119045
start: 1213
end: 1872
orientation: inline

stuffer
type: SequenceAnnotation
persistent identity: annotation2169686
display id: annotation2169686
version: 1
top level: BBa_J119045
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119045/annotation2169686/range2169686/1

https://synbiohub.org/public/igem/BBa_J119045/annotation2169686/range2169686/1
type: Range
persistent identity: range2169686
display id: range2169686
version: 1
direction: direction/forward
top level: BBa_J119045
start: 2155
end: 2167
orientation: inline

B0025 terminator
type: SequenceAnnotation
persistent identity: annotation2169684
display id: annotation2169684
version: 1
top level: BBa_J119045
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119045/annotation2169684/range2169684/1

https://synbiohub.org/public/igem/BBa_J119045/annotation2169684/range2169684/1
type: Range
persistent identity: range2169684
display id: range2169684
version: 1
direction: direction/reverse
top level: BBa_J119045
start: 22
end: 205
orientation: inline

B0010 terminator
type: SequenceAnnotation
persistent identity: annotation2169685
display id: annotation2169685
version: 1
top level: BBa_J119045
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119045/annotation2169685/range2169685/1

https://synbiohub.org/public/igem/BBa_J119045/annotation2169685/range2169685/1
type: Range
persistent identity: range2169685
display id: range2169685
version: 1
direction: direction/forward
top level: BBa_J119045
start: 2094
end: 2133
orientation: inline

pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation2169683
display id: annotation2169683
version: 1
top level: BBa_J119045
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119045/annotation2169683/range2169683/1

https://synbiohub.org/public/igem/BBa_J119045/annotation2169683/range2169683/1
type: Range
persistent identity: range2169683
display id: range2169683
version: 1
direction: direction/reverse
top level: BBa_J119045
start: 313
end: 927
orientation: inline

VR primer
type: SequenceAnnotation
persistent identity: annotation2169680
display id: annotation2169680
version: 1
top level: BBa_J119045
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119045/annotation2169680/range2169680/1

https://synbiohub.org/public/igem/BBa_J119045/annotation2169680/range2169680/1
type: Range
persistent identity: range2169680
display id: range2169680
version: 1
direction: direction/reverse
top level: BBa_J119045
start: 208
end: 227
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2169679
display id: annotation2169679
version: 1
top level: BBa_J119045
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119045/annotation2169679/range2169679/1

https://synbiohub.org/public/igem/BBa_J119045/annotation2169679/range2169679/1
type: Range
persistent identity: range2169679
display id: range2169679
version: 1
direction: direction/forward
top level: BBa_J119045
start: 1
end: 21
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2169681
display id: annotation2169681
version: 1
top level: BBa_J119045
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119045/annotation2169681/range2169681/1

https://synbiohub.org/public/igem/BBa_J119045/annotation2169681/range2169681/1
type: Range
persistent identity: range2169681
display id: range2169681
version: 1
direction: direction/forward
top level: BBa_J119045
start: 1984
end: 2003
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2169678
display id: annotation2169678
version: 1
top level: BBa_J119045
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119045/annotation2169678/range2169678/1

https://synbiohub.org/public/igem/BBa_J119045/annotation2169678/range2169678/1
type: Range
persistent identity: range2169678
display id: range2169678
version: 1
direction: direction/forward
top level: BBa_J119045
start: 2134
end: 2155
orientation: inline

BBa_K1680015
type: ComponentDefinition
persistent identity: BBa K1680015
display id: BBa_K1680015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS315 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:36:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bakteria and URA3 gene for yeast selection.
mutable notes: contains an RFC10 compatible multiple cloning site, no restrictions sites for RFC10 in the Backbone. ADH Terminator after MCS.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ampR
sequence annotation: lacZalpha
sequence annotation: T3 promotor
sequence annotation: LEU2
sequence annotation: f1 origin
sequence annotation: lac promotor
sequence annotation: ampR promotor
sequence annotation: LEU2 promotor
sequence annotation: T7 promotor
sequence annotation: pUC origin
sequence annotation: RFC10
sequence annotation: CEN/ARS yeast centromere
sequence: https://synbiohub.org/public/igem/BBa_K1680015_sequence/1

ampR
type: SequenceAnnotation
persistent identity: annotation2466201
display id: annotation2466201
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466201/range2466201/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466201/range2466201/1
type: Range
persistent identity: range2466201
display id: range2466201
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 4405
end: 5265
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466196
display id: annotation2466196
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466196/range2466196/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466196/range2466196/1
type: Range
persistent identity: range2466196
display id: range2466196
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3186
end: 3248
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466197
display id: annotation2466197
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466197/range2466197/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466197/range2466197/1
type: Range
persistent identity: range2466197
display id: range2466197
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3205
end: 3223
orientation: inline

LEU2
type: SequenceAnnotation
persistent identity: annotation2466188
display id: annotation2466188
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466188/range2466188/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466188/range2466188/1
type: Range
persistent identity: range2466188
display id: range2466188
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 663
end: 1757
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2466190
display id: annotation2466190
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466190/range2466190/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466190/range2466190/1
type: Range
persistent identity: range2466190
display id: range2466190
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 2474
end: 2929
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466199
display id: annotation2466199
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466199/range2466199/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466199/range2466199/1
type: Range
persistent identity: range2466199
display id: range2466199
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3292
end: 3322
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466191
display id: annotation2466191
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466191/range2466191/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466191/range2466191/1
type: Range
persistent identity: range2466191
display id: range2466191
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 2712
end: 3128
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466202
display id: annotation2466202
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466202/range2466202/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466202/range2466202/1
type: Range
persistent identity: range2466202
display id: range2466202
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 5266
end: 5370
orientation: inline

LEU2 promotor
type: SequenceAnnotation
persistent identity: annotation2466189
display id: annotation2466189
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466189/range2466189/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466189/range2466189/1
type: Range
persistent identity: range2466189
display id: range2466189
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 1770
end: 2174
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466192
display id: annotation2466192
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466192/range2466192/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466192/range2466192/1
type: Range
persistent identity: range2466192
display id: range2466192
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 3097
end: 3115
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466200
display id: annotation2466200
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466200/range2466200/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466200/range2466200/1
type: Range
persistent identity: range2466200
display id: range2466200
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3646
end: 4234
orientation: inline

RFC10
type: SequenceAnnotation
persistent identity: annotation2466193
display id: annotation2466193
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466193/range2466193/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466193/range2466193/1
type: Range
persistent identity: range2466193
display id: range2466193
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 3134
end: 3176
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466204
display id: annotation2466204
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466204/range2466204/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466204/range2466204/1
type: Range
persistent identity: range2466204
display id: range2466204
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 5407
end: 5910
orientation: inline

BBa_J176128
type: ComponentDefinition
persistent identity: BBa J176128
display id: BBa_J176128
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcDNA3.1+ neo
creator: Brady Laughlin
created: 2011-10-25T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pcDNA3.1+ neo/ ΔpCMV, based on pcDNA3.1+; no promoter; amp, neo
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pUC origin
sequence annotation: bla
sequence annotation: f1 origin
sequence annotation: SpeI EcoRI NotI XbaI
sequence annotation: Ampicillin resistance
sequence annotation: SV40
sequence annotation: Neomycin resistance
sequence annotation: CMV promoter remnant
sequence annotation: BGH
sequence annotation: DD122B
sequence: https://synbiohub.org/public/igem/BBa_J176128_sequence/1

pUC origin
type: SequenceAnnotation
persistent identity: annotation2167200
display id: annotation2167200
version: 1
top level: BBa_J176128
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167200/range2167200/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167200/range2167200/1
type: Range
persistent identity: range2167200
display id: range2167200
version: 1
direction: direction/reverse
top level: BBa_J176128
start: 2931
end: 3601
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2167202
display id: annotation2167202
version: 1
top level: BBa_J176128
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167202/range2167202/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167202/range2167202/1
type: Range
persistent identity: range2167202
display id: range2167202
version: 1
direction: direction/reverse
top level: BBa_J176128
start: 4607
end: 4705
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2167196
display id: annotation2167196
version: 1
top level: BBa_J176128
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167196/range2167196/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167196/range2167196/1
type: Range
persistent identity: range2167196
display id: range2167196
version: 1
direction: direction/forward
top level: BBa_J176128
start: 612
end: 1040
orientation: inline

SpeI EcoRI NotI XbaI
type: SequenceAnnotation
persistent identity: annotation2167193
display id: annotation2167193
version: 1
top level: BBa_J176128
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167193/range2167193/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167193/range2167193/1
type: Range
persistent identity: range2167193
display id: range2167193
version: 1
direction: direction/forward
top level: BBa_J176128
start: 250
end: 324
orientation: inline

Ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation2167201
display id: annotation2167201
version: 1
top level: BBa_J176128
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167201/range2167201/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167201/range2167201/1
type: Range
persistent identity: range2167201
display id: range2167201
version: 1
direction: direction/reverse
top level: BBa_J176128
start: 3746
end: 4606
orientation: inline

SV40
type: SequenceAnnotation
persistent identity: annotation2167199
display id: annotation2167199
version: 1
top level: BBa_J176128
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167199/range2167199/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167199/range2167199/1
type: Range
persistent identity: range2167199
display id: range2167199
version: 1
direction: direction/forward
top level: BBa_J176128
start: 2418
end: 2548
orientation: inline

Neomycin resistance
type: SequenceAnnotation
persistent identity: annotation2167198
display id: annotation2167198
version: 1
top level: BBa_J176128
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167198/range2167198/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167198/range2167198/1
type: Range
persistent identity: range2167198
display id: range2167198
version: 1
direction: direction/forward
top level: BBa_J176128
start: 1450
end: 2244
orientation: inline

CMV promoter remnant
type: SequenceAnnotation
persistent identity: annotation2167192
display id: annotation2167192
version: 1
top level: BBa_J176128
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167192/range2167192/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167192/range2167192/1
type: Range
persistent identity: range2167192
display id: range2167192
version: 1
direction: direction/forward
top level: BBa_J176128
start: 232
end: 249
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2167195
display id: annotation2167195
version: 1
top level: BBa_J176128
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167195/range2167195/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167195/range2167195/1
type: Range
persistent identity: range2167195
display id: range2167195
version: 1
direction: direction/forward
top level: BBa_J176128
start: 342
end: 566
orientation: inline

DD122B
type: SequenceAnnotation
persistent identity: annotation2167203
display id: annotation2167203
version: 1
top level: BBa_J176128
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167203/range2167203/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167203/range2167203/1
type: Range
persistent identity: range2167203
display id: range2167203
version: 1
direction: direction/forward
top level: BBa_J176128
start: 186
end: 203
orientation: inline

SV40
type: SequenceAnnotation
persistent identity: annotation2167197
display id: annotation2167197
version: 1
top level: BBa_J176128
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167197/range2167197/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167197/range2167197/1
type: Range
persistent identity: range2167197
display id: range2167197
version: 1
direction: direction/forward
top level: BBa_J176128
start: 1045
end: 1388
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2167194
display id: annotation2167194
version: 1
top level: BBa_J176128
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176128/annotation2167194/range2167194/1

https://synbiohub.org/public/igem/BBa_J176128/annotation2167194/range2167194/1
type: Range
persistent identity: range2167194
display id: range2167194
version: 1
direction: direction/reverse
top level: BBa_J176128
start: 336
end: 353
orientation: inline

BBa_K514004
type: ComponentDefinition
persistent identity: BBa K514004
display id: BBa_K514004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GeneArt pMK-RQ (Kan)
creator: Elena Moreno Mart??nez
created: 2011-09-24T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _679_
m user id: 0
owner id: 8366
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A plasmid backbone in which GeneArt often sends the constructions they're asked for, with kanamicine resistance, and restriction sites for SfiI, NarI, DraII, NcoI, Eco57I, and Col E1 origin.
mutable notes: None
mutable provenance: GeneArt, DNA synthesis company
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K514004_sequence/1

pSB8S15FRT
type: ComponentDefinition
persistent identity: BBa K864023
display id: BBa_K864023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8S15(FRT) is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864023_sequence/1

BBa_K1110000
type: ComponentDefinition
persistent identity: BBa K1110000
display id: BBa_K1110000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAPzalphaA+RFP
creator: Lydia Studdard
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1421_
m user id: 0
owner id: 16826
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plasmid pGAPzalpha A standardized according to iGEM standards, with RFP sequence.
mutable notes: Designed to conform to iGEM standards
mutable provenance: pGAPzalpha A, Saccharomyces cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: suffix
sequence annotation: α factor secretion signal
sequence annotation: GAP Promotor
sequence annotation: prefix
sequence: https://synbiohub.org/public/igem/BBa_K1110000_sequence/1

suffix
type: SequenceAnnotation
persistent identity: annotation2352626
display id: annotation2352626
version: 1
top level: BBa_K1110000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1110000/annotation2352626/range2352626/1

https://synbiohub.org/public/igem/BBa_K1110000/annotation2352626/range2352626/1
type: Range
persistent identity: range2352626
display id: range2352626
version: 1
direction: direction/forward
top level: BBa_K1110000
start: 1
end: 21
orientation: inline

α factor secretion signal
type: SequenceAnnotation
persistent identity: annotation2352934
display id: annotation2352934
version: 1
top level: BBa_K1110000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1110000/annotation2352934/range2352934/1

https://synbiohub.org/public/igem/BBa_K1110000/annotation2352934/range2352934/1
type: Range
persistent identity: range2352934
display id: range2352934
version: 1
direction: direction/forward
top level: BBa_K1110000
start: 2846
end: 3112
orientation: inline

GAP Promotor
type: SequenceAnnotation
persistent identity: annotation2352905
display id: annotation2352905
version: 1
top level: BBa_K1110000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1110000/annotation2352905/range2352905/1

https://synbiohub.org/public/igem/BBa_K1110000/annotation2352905/range2352905/1
type: Range
persistent identity: range2352905
display id: range2352905
version: 1
direction: direction/forward
top level: BBa_K1110000
start: 2360
end: 2837
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2352627
display id: annotation2352627
version: 1
top level: BBa_K1110000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1110000/annotation2352627/range2352627/1

https://synbiohub.org/public/igem/BBa_K1110000/annotation2352627/range2352627/1
type: Range
persistent identity: range2352627
display id: range2352627
version: 1
direction: direction/forward
top level: BBa_K1110000
start: 3113
end: 3134
orientation: inline

pSac-Cm_BB
type: ComponentDefinition
persistent identity: BBa K818000
display id: BBa_K818000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Integration vector for Bacillus subtilis derived from pSac-Cm
creator: Emeraldo Jo
created: 2012-08-21T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1076_
m user id: 0
owner id: 11906
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The plasmid is designed to integrate a cloned insert into the B. subtilis 168 chromosome at the sacA locus. The user inserts the fragment inside the MCS whi
mutable notes: Biobrick ready
mutable provenance: MolGen Group (University of Groningen)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick prefix
sequence annotation: sacA'
sequence annotation: BioBrick suffix
sequence annotation: bla
sequence annotation: 'sacA
sequence annotation: BBa_B0015
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K818000_sequence/1

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2203458
display id: annotation2203458
version: 1
top level: pSac-Cm_BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203458/range2203458/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203458/range2203458/1
type: Range
persistent identity: range2203458
display id: range2203458
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 5207
end: 5223
orientation: inline

sacA'
type: SequenceAnnotation
persistent identity: annotation2203456
display id: annotation2203456
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203456/range2203456/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203456/range2203456/1
type: Range
persistent identity: range2203456
display id: range2203456
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 3187
end: 3930
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2203452
display id: annotation2203452
version: 1
top level: pSac-Cm_BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203452/range2203452/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203452/range2203452/1
type: Range
persistent identity: range2203452
display id: range2203452
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 1
end: 17
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2203455
display id: annotation2203455
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203455/range2203455/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203455/range2203455/1
type: Range
persistent identity: range2203455
display id: range2203455
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 1324
end: 2196
orientation: inline

'sacA
type: SequenceAnnotation
persistent identity: annotation2203454
display id: annotation2203454
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203454/range2203454/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203454/range2203454/1
type: Range
persistent identity: range2203454
display id: range2203454
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 181
end: 990
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2203453
display id: annotation2203453
version: 1
top level: pSac-Cm_BB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203453/range2203453/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203453/range2203453/1
type: Range
persistent identity: range2203453
display id: range2203453
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 18
end: 170
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2203457
display id: annotation2203457
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203457/range2203457/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203457/range2203457/1
type: Range
persistent identity: range2203457
display id: range2203457
version: 1
direction: direction/reverse
top level: pSac-Cm_BB
start: 4249
end: 4911
orientation: inline

BBa_K1441001
type: ComponentDefinition
persistent identity: BBa K1441001
display id: BBa_K1441001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PGAPz alpha C + Linker
creator: Reza Alavi
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1819_
m user id: 0
owner id: 18396
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This plasmid is PGAPzα C + modified Multiple Cloning Site(MCS) (Linker) fixed according to iGEM standards. We replaced old multiple cloning cite with new modified multiple cloning site, which has new cutting sites according to iGEM standard. The original MCS has ECORI, KpnI, NotI and XbaI restriction enzyme binding sites compared to the new MCS we designed in which there are ECORI, NotI, XbaI, SpeI and PstI restriction enzyme cutting sites. PGAPzα C + Linker has α-secretion factor before MCS which allows yeast cells and some Eukaryotic cells to secrete the protein into the outside of the cell membrane. Also after MCS there are MYC epitope and 6x histag that are used to tag the protein so it can be easily identified by westernblot technique.
mutable notes: we had to design the new MCS the way that would not create stop codon while translating out protein.
mutable provenance: no
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: TTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTC
sequence annotation: GAACAAAAACTCATCTCAGAAGAGGATCTG
sequence annotation: ATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAA
sequence annotation: CATCATCATCATCATCAT
sequence: https://synbiohub.org/public/igem/BBa_K1441001_sequence/1

TTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTC
type: SequenceAnnotation
persistent identity: annotation2428810
display id: annotation2428810
version: 1
top level: BBa_K1441001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1441001/annotation2428810/range2428810/1

https://synbiohub.org/public/igem/BBa_K1441001/annotation2428810/range2428810/1
type: Range
persistent identity: range2428810
display id: range2428810
version: 1
direction: direction/forward
top level: BBa_K1441001
start: 7
end: 483
orientation: inline

GAACAAAAACTCATCTCAGAAGAGGATCTG
type: SequenceAnnotation
persistent identity: annotation2428812
display id: annotation2428812
version: 1
top level: BBa_K1441001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1441001/annotation2428812/range2428812/1

https://synbiohub.org/public/igem/BBa_K1441001/annotation2428812/range2428812/1
type: Range
persistent identity: range2428812
display id: range2428812
version: 1
direction: direction/forward
top level: BBa_K1441001
start: 842
end: 871
orientation: inline

ATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAA
type: SequenceAnnotation
persistent identity: annotation2428811
display id: annotation2428811
version: 1
top level: BBa_K1441001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1441001/annotation2428811/range2428811/1

https://synbiohub.org/public/igem/BBa_K1441001/annotation2428811/range2428811/1
type: Range
persistent identity: range2428811
display id: range2428811
version: 1
direction: direction/forward
top level: BBa_K1441001
start: 493
end: 759
orientation: inline

CATCATCATCATCATCAT
type: SequenceAnnotation
persistent identity: annotation2428813
display id: annotation2428813
version: 1
top level: BBa_K1441001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1441001/annotation2428813/range2428813/1

https://synbiohub.org/public/igem/BBa_K1441001/annotation2428813/range2428813/1
type: Range
persistent identity: range2428813
display id: range2428813
version: 1
direction: direction/forward
top level: BBa_K1441001
start: 887
end: 904
orientation: inline

BBa_J23018
type: ComponentDefinition
persistent identity: BBa J23018
display id: BBa_J23018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"
creator: John Anderson
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Variant of pBca1021 (J61003) with GFP inserted. In practice, it is a E0040 basic part just in a different pSB1A* plasmid.
mutable notes: N/A
mutable provenance: J61003 and E0040
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence annotation: pBR322 origin
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: BBa_R0040 (Ptet)
sequence annotation: BioBrick suffix
sequence annotation: RBS
sequence annotation: EcoRI
sequence annotation: XbaI
sequence annotation: BBa_B0015 terminator
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: NotI
sequence annotation: rrnB T1 terminator
sequence annotation: BBa_B0034
sequence annotation: pMB1 origin
sequence annotation: Ptet
sequence: https://synbiohub.org/public/igem/BBa_J23018_sequence/1

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1975734
display id: annotation1975734
version: 1
top level: BBa_J23018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975734/range1975734/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975734/range1975734/1
type: Range
persistent identity: range1975734
display id: range1975734
version: 1
direction: direction/reverse
top level: BBa_J23018
start: 957
end: 1817
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1893437
display id: annotation1893437
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1893437/range1893437/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1893437/range1893437/1
type: Range
persistent identity: range1893437
display id: range1893437
version: 1
direction: direction/reverse
top level: BBa_J23018
start: 183
end: 802
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975760
display id: annotation1975760
version: 1
top level: BBa_J23018
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975760/range1975760/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975760/range1975760/1
type: Range
persistent identity: range1975760
display id: range1975760
version: 1
direction: direction/forward
top level: BBa_J23018
start: 1940
end: 1959
orientation: inline

BBa_R0040 (Ptet)
type: SequenceAnnotation
persistent identity: annotation1893436
display id: annotation1893436
version: 1
top level: BBa_J23018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1893436/range1893436/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1893436/range1893436/1
type: Range
persistent identity: range1893436
display id: range1893436
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2217
end: 2270
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975737
display id: annotation1975737
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975737/range1975737/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975737/range1975737/1
type: Range
persistent identity: range1975737
display id: range1975737
version: 1
direction: direction/forward
top level: BBa_J23018
start: 1
end: 21
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975764
display id: annotation1975764
version: 1
top level: BBa_J23018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975764/range1975764/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975764/range1975764/1
type: Range
persistent identity: range1975764
display id: range1975764
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2279
end: 2290
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975738
display id: annotation1975738
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975738/range1975738/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975738/range1975738/1
type: Range
persistent identity: range1975738
display id: range1975738
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2058
end: 2063
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975740
display id: annotation1975740
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975740/range1975740/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975740/range1975740/1
type: Range
persistent identity: range1975740
display id: range1975740
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2292
end: 2297
orientation: inline

BBa_B0015 terminator
type: SequenceAnnotation
persistent identity: annotation1893435
display id: annotation1893435
version: 1
top level: BBa_J23018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1893435/range1893435/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1893435/range1893435/1
type: Range
persistent identity: range1893435
display id: range1893435
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2080
end: 2208
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975761
display id: annotation1975761
version: 1
top level: BBa_J23018
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975761/range1975761/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975761/range1975761/1
type: Range
persistent identity: range1975761
display id: range1975761
version: 1
direction: direction/reverse
top level: BBa_J23018
start: 79
end: 98
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1975735
display id: annotation1975735
version: 1
top level: BBa_J23018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975735/range1975735/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975735/range1975735/1
type: Range
persistent identity: range1975735
display id: range1975735
version: 1
direction: direction/reverse
top level: BBa_J23018
start: 1859
end: 1887
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975739
display id: annotation1975739
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975739/range1975739/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975739/range1975739/1
type: Range
persistent identity: range1975739
display id: range1975739
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2064
end: 2071
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975736
display id: annotation1975736
version: 1
top level: BBa_J23018
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975736/range1975736/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975736/range1975736/1
type: Range
persistent identity: range1975736
display id: range1975736
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2091
end: 2134
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1975763
display id: annotation1975763
version: 1
top level: BBa_J23018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975763/range1975763/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975763/range1975763/1
type: Range
persistent identity: range1975763
display id: range1975763
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2279
end: 2290
orientation: inline

pMB1 origin
type: SequenceAnnotation
persistent identity: annotation1934517
display id: annotation1934517
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1934517/range1934517/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1934517/range1934517/1
type: Range
persistent identity: range1934517
display id: range1934517
version: 1
direction: direction/reverse
top level: BBa_J23018
start: 180
end: 895
orientation: inline

Ptet
type: SequenceAnnotation
persistent identity: annotation1975765
display id: annotation1975765
version: 1
top level: BBa_J23018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975765/range1975765/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975765/range1975765/1
type: Range
persistent identity: range1975765
display id: range1975765
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2217
end: 2270
orientation: inline

BBa_K1033204
type: ComponentDefinition
persistent identity: BBa K1033204
display id: BBa_K1033204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance
creator: Viktor Blomkvist
created: 2013-08-22T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1340_
m user id: 0
owner id: 17571
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: E. coli Lactobacilli shuttle vector pSBLb4C15 is a shuttle vector containing the ori pSH71 originating from the plasmid pJP059 which was taken from Lactococcus lactis. It has a red insert.
mutable notes: Had to make sure it was compatible with biobrick standard 10
mutable provenance: Ori in pEL4C15 replaced with ori from pJP059. Chloramphenicol resistance replaced with erythromycin resistance.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: mrfp1
sequence annotation: BBa_K1033200
sequence annotation: BBa_E1010
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_K1033204_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation2332094
display id: annotation2332094
version: 1
top level: BBa_K1033204
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033204/annotation2332094/range2332094/1

https://synbiohub.org/public/igem/BBa_K1033204/annotation2332094/range2332094/1
type: Range
persistent identity: range2332094
display id: range2332094
version: 1
direction: direction/forward
top level: BBa_K1033204
start: 1
end: 675
orientation: inline

BBa_K1033200
type: SequenceAnnotation
persistent identity: annotation2332097
display id: annotation2332097
version: 1
top level: BBa_K1033204
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1033204/annotation2332097/range2332097/1

https://synbiohub.org/public/igem/BBa_K1033204/annotation2332097/range2332097/1
type: Range
persistent identity: range2332097
display id: range2332097
version: 1
direction: direction/reverse
top level: BBa_K1033204
start: 1
end: 706
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2332095
display id: annotation2332095
version: 1
top level: BBa_K1033204
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1033204/annotation2332095/range2332095/1

https://synbiohub.org/public/igem/BBa_K1033204/annotation2332095/range2332095/1
type: Range
persistent identity: range2332095
display id: range2332095
version: 1
direction: direction/reverse
top level: BBa_K1033204
start: 1
end: 706
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2332096
display id: annotation2332096
version: 1
top level: BBa_K1033204
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1033204/annotation2332096/range2332096/1

https://synbiohub.org/public/igem/BBa_K1033204/annotation2332096/range2332096/1
type: Range
persistent identity: range2332096
display id: range2332096
version: 1
direction: direction/forward
top level: BBa_K1033204
start: 682
end: 706
orientation: inline

BBa_K187001
type: ComponentDefinition
persistent identity: BBa K187001
display id: BBa_K187001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBA Universal Plasmid for BioBytes Gene Assembly System
creator: Team BioBytes, University of Alberta
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _286_
m user id: 0
owner id: 4048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The boundary sequences required for sticky end production for the BioBytes gene assembly system are acquired by inserting gene sequences destined to become bytes into either of the host plasmids pAB or pBA. Both are derivatives of pUC19 with the following key modifications: 1. Replacement of the pUC19 MCS with the MCS shown in figure 1. 2. Elimination of the Z alpha fragment 3. Elimination of the restriction sites Bts1 and BspQ1 Functional features include: 1. The ability to adapt biobricks to the Byte format using the Xba1 and Pst1 sites contained in the MCS. 2. The ability to adapt the in the ASKA collection (Kitagawa 2005) to the Byte format (4000+ E. coli K12 ORFs). ORFs are cloned into an expression vector, accompanied by a 6xHis tag at the N-terminus and a GFP fusion on the C-terminus. ASKA parts can be freed by digestion with SfiI, leaving noncomplementary 3 bp 3' sticky ends, which can be cloned directionally into the MCS after digestion with BstAPI. Since the ASKA parts are freed from the interior of the CDS, the MCS provides a start codon 10 bp downstream of the RBS, and a stop codon (TGA) downstream of the insertion. ORFs cloned from ASKA plasmids replace the N-terminal methionine of the predicted ORF with Met-Ala-Leu-Arg-Ala, and append Cys-Leu at the C-terminus. 3. Other potentially useful restriction sites have been included in the MCS as well as an RBS consensus positioned 8 bp upstream of the ATG used for ORF???s derived from 1. And 2 4. The ability to select for insert-containing constructs through the linearization of parental contaminants by cleavage with the rare- cutters SwaI and PmeI contained in the MCS. 5. Two routes for the production of 12 base overhangs by PCR using universal primers: A) PCR followed by nicking using the engineered enzymes Nb.BbvCI and Nb.BtsI. B) PCR followed by removal of a primer-encoded uracil residue using BioLab???s UserTM system. Primer positions and sequences are indicated for both.
mutable notes: Using these plasmids as well as universal primer sequences for the PCR extraction of bricks from the plasmids anyone will be able to use this system for their own rapid assembly. The design ensures that the existing catalog of BioBrick parts is compatible with the system, and can be easily cloned into the universal plasmids for brick generation. We believe that this new system is ready for both use and formalization, and we have prepared a BBF RFC detailing the use of the BioBytes system
mutable provenance: Derivative of pUC19
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: B overhang
sequence annotation: A overhang
sequence annotation: amp
sequence annotation: EcoR1
sequence annotation: BA cassette
sequence annotation: RBS
sequence annotation: PstI
sequence annotation: XbaI
sequence annotation: oriT
sequence: https://synbiohub.org/public/igem/BBa_K187001_sequence/1

B overhang
type: SequenceAnnotation
persistent identity: annotation2042805
display id: annotation2042805
version: 1
top level: BBa_K187001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K187001/annotation2042805/range2042805/1

https://synbiohub.org/public/igem/BBa_K187001/annotation2042805/range2042805/1
type: Range
persistent identity: range2042805
display id: range2042805
version: 1
direction: direction/forward
top level: BBa_K187001
start: 771
end: 782
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation2042806
display id: annotation2042806
version: 1
top level: BBa_K187001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K187001/annotation2042806/range2042806/1

https://synbiohub.org/public/igem/BBa_K187001/annotation2042806/range2042806/1
type: Range
persistent identity: range2042806
display id: range2042806
version: 1
direction: direction/reverse
top level: BBa_K187001
start: 845
end: 856
orientation: inline

amp
type: SequenceAnnotation
persistent identity: annotation2042803
display id: annotation2042803
version: 1
top level: BBa_K187001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187001/annotation2042803/range2042803/1

https://synbiohub.org/public/igem/BBa_K187001/annotation2042803/range2042803/1
type: Range
persistent identity: range2042803
display id: range2042803
version: 1
direction: direction/forward
top level: BBa_K187001
start: 1012
end: 1872
orientation: inline

EcoR1
type: SequenceAnnotation
persistent identity: annotation2042800
display id: annotation2042800
version: 1
top level: BBa_K187001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187001/annotation2042800/range2042800/1

https://synbiohub.org/public/igem/BBa_K187001/annotation2042800/range2042800/1
type: Range
persistent identity: range2042800
display id: range2042800
version: 1
direction: direction/forward
top level: BBa_K187001
start: 750
end: 755
orientation: inline

BA cassette
type: SequenceAnnotation
persistent identity: annotation2042804
display id: annotation2042804
version: 1
top level: BBa_K187001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187001/annotation2042804/range2042804/1

https://synbiohub.org/public/igem/BBa_K187001/annotation2042804/range2042804/1
type: Range
persistent identity: range2042804
display id: range2042804
version: 1
direction: direction/forward
top level: BBa_K187001
start: 750
end: 881
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2061896
display id: annotation2061896
version: 1
top level: BBa_K187001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K187001/annotation2061896/range2061896/1

https://synbiohub.org/public/igem/BBa_K187001/annotation2061896/range2061896/1
type: Range
persistent identity: range2061896
display id: range2061896
version: 1
direction: direction/reverse
top level: BBa_K187001
start: 785
end: 791
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2042802
display id: annotation2042802
version: 1
top level: BBa_K187001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187001/annotation2042802/range2042802/1

https://synbiohub.org/public/igem/BBa_K187001/annotation2042802/range2042802/1
type: Range
persistent identity: range2042802
display id: range2042802
version: 1
direction: direction/forward
top level: BBa_K187001
start: 837
end: 842
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2042801
display id: annotation2042801
version: 1
top level: BBa_K187001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187001/annotation2042801/range2042801/1

https://synbiohub.org/public/igem/BBa_K187001/annotation2042801/range2042801/1
type: Range
persistent identity: range2042801
display id: range2042801
version: 1
direction: direction/forward
top level: BBa_K187001
start: 792
end: 797
orientation: inline

oriT
type: SequenceAnnotation
persistent identity: annotation2042807
display id: annotation2042807
version: 1
top level: BBa_K187001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187001/annotation2042807/range2042807/1

https://synbiohub.org/public/igem/BBa_K187001/annotation2042807/range2042807/1
type: Range
persistent identity: range2042807
display id: range2042807
version: 1
direction: direction/reverse
top level: BBa_K187001
start: 28
end: 648
orientation: inline

BBa_M36092
type: ComponentDefinition
persistent identity: BBa M36092
display id: BBa_M36092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S. cerevisiae actuator test rig
creator: Joseph Shih
created: 2013-05-07T11:00:00Z
modified: 2015-05-08T01:14:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 10436
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: S. cerevisae test vector for actuators. Has a Gal1 promoter which is galactose inducible and glucose repressible. Comes with high copy number origin, kanamycin resistance, and leucine yeast selection; can replace with low copy number origin if ordering from DNA 2.0.
mutable notes: Has S. cerevisiae specific LEU selection and 2 um origin of replication allowing for 50-100 copies of the plasmid in yeast cells.
mutable provenance: Vector is based off of pJ1201-03c plasmid developed by DNA2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Gal1 promoter
sequence annotation: Leu
sequence annotation: Pamp
sequence annotation: KanR
sequence annotation: student actuators go here
sequence annotation: Pleu
sequence annotation: 2um ori
sequence annotation: pUCori
sequence annotation: Leu polyA
sequence: https://synbiohub.org/public/igem/BBa_M36092_sequence/1

Gal1 promoter
type: SequenceAnnotation
persistent identity: annotation2217235
display id: annotation2217235
version: 1
top level: BBa_M36092
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_M36092/annotation2217235/range2217235/1

https://synbiohub.org/public/igem/BBa_M36092/annotation2217235/range2217235/1
type: Range
persistent identity: range2217235
display id: range2217235
version: 1
direction: direction/forward
top level: BBa_M36092
start: 1757
end: 2171
orientation: inline

Leu
type: SequenceAnnotation
persistent identity: annotation2217238
display id: annotation2217238
version: 1
top level: BBa_M36092
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M36092/annotation2217238/range2217238/1

https://synbiohub.org/public/igem/BBa_M36092/annotation2217238/range2217238/1
type: Range
persistent identity: range2217238
display id: range2217238
version: 1
direction: direction/forward
top level: BBa_M36092
start: 3399
end: 4493
orientation: inline

Pamp
type: SequenceAnnotation
persistent identity: annotation2217232
display id: annotation2217232
version: 1
top level: BBa_M36092
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_M36092/annotation2217232/range2217232/1

https://synbiohub.org/public/igem/BBa_M36092/annotation2217232/range2217232/1
type: Range
persistent identity: range2217232
display id: range2217232
version: 1
direction: direction/forward
top level: BBa_M36092
start: 1
end: 128
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2217233
display id: annotation2217233
version: 1
top level: BBa_M36092
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M36092/annotation2217233/range2217233/1

https://synbiohub.org/public/igem/BBa_M36092/annotation2217233/range2217233/1
type: Range
persistent identity: range2217233
display id: range2217233
version: 1
direction: direction/forward
top level: BBa_M36092
start: 129
end: 938
orientation: inline

student actuators go here
type: SequenceAnnotation
persistent identity: annotation2217236
display id: annotation2217236
version: 1
top level: BBa_M36092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36092/annotation2217236/range2217236/1

https://synbiohub.org/public/igem/BBa_M36092/annotation2217236/range2217236/1
type: Range
persistent identity: range2217236
display id: range2217236
version: 1
direction: direction/forward
top level: BBa_M36092
start: 2173
end: 2883
orientation: inline

Pleu
type: SequenceAnnotation
persistent identity: annotation2217237
display id: annotation2217237
version: 1
top level: BBa_M36092
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_M36092/annotation2217237/range2217237/1

https://synbiohub.org/public/igem/BBa_M36092/annotation2217237/range2217237/1
type: Range
persistent identity: range2217237
display id: range2217237
version: 1
direction: direction/forward
top level: BBa_M36092
start: 2899
end: 3398
orientation: inline

2um ori
type: SequenceAnnotation
persistent identity: annotation2217240
display id: annotation2217240
version: 1
top level: BBa_M36092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36092/annotation2217240/range2217240/1

https://synbiohub.org/public/igem/BBa_M36092/annotation2217240/range2217240/1
type: Range
persistent identity: range2217240
display id: range2217240
version: 1
direction: direction/forward
top level: BBa_M36092
start: 4598
end: 6043
orientation: inline

pUCori
type: SequenceAnnotation
persistent identity: annotation2217234
display id: annotation2217234
version: 1
top level: BBa_M36092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36092/annotation2217234/range2217234/1

https://synbiohub.org/public/igem/BBa_M36092/annotation2217234/range2217234/1
type: Range
persistent identity: range2217234
display id: range2217234
version: 1
direction: direction/forward
top level: BBa_M36092
start: 939
end: 1742
orientation: inline

Leu polyA
type: SequenceAnnotation
persistent identity: annotation2217239
display id: annotation2217239
version: 1
top level: BBa_M36092
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_M36092/annotation2217239/range2217239/1

https://synbiohub.org/public/igem/BBa_M36092/annotation2217239/range2217239/1
type: Range
persistent identity: range2217239
display id: range2217239
version: 1
direction: direction/forward
top level: BBa_M36092
start: 4494
end: 4593
orientation: inline

BBa_K1441002
type: ComponentDefinition
persistent identity: BBa K1441002
display id: BBa_K1441002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PGAPz alpha A + Linker
creator: Julia Dave
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1819_
m user id: 0
owner id: 23093
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This plasmid is PGAPzα C + modified Multiple Cloning Site(MCS) (Linker) fixed according to iGEM standards. We replaced old multiple cloning cite with new modified multiple cloning site, which has new cutting sites according to iGEM standard. The original MCS has ECORI, KpnI, NotI and XbaI restriction enzyme binding sites compared to the new MCS we designed in which there are ECORI, NotI, XbaI, SpeI and PstI restriction enzyme cutting sites. PGAPzα C + Linker has α-secretion factor before MCS which allows yeast cells and some Eukaryotic cells to secrete the protein into the outside of the cell membrane. Also after MCS there are MYC epitope and 6x histag that are used to tag the protein so it can be easily identified by westernblot technique.
mutable notes: We replaced old multiple cloning cite with new modified multiple cloning site, which has new cutting sites according to iGEM standard.
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: CATCATCATCATCATCAT
sequence annotation: ATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAA
sequence annotation: GAACAAAAACTCATCTCAGAAGAGGATCTG
sequence annotation: TTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTC
sequence annotation: GATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTTCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTT
sequence: https://synbiohub.org/public/igem/BBa_K1441002_sequence/1

CATCATCATCATCATCAT
type: SequenceAnnotation
persistent identity: annotation2428808
display id: annotation2428808
version: 1
top level: BBa_K1441002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1441002/annotation2428808/range2428808/1

https://synbiohub.org/public/igem/BBa_K1441002/annotation2428808/range2428808/1
type: Range
persistent identity: range2428808
display id: range2428808
version: 1
direction: direction/forward
top level: BBa_K1441002
start: 882
end: 899
orientation: inline

ATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAA
type: SequenceAnnotation
persistent identity: annotation2428806
display id: annotation2428806
version: 1
top level: BBa_K1441002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1441002/annotation2428806/range2428806/1

https://synbiohub.org/public/igem/BBa_K1441002/annotation2428806/range2428806/1
type: Range
persistent identity: range2428806
display id: range2428806
version: 1
direction: direction/forward
top level: BBa_K1441002
start: 493
end: 759
orientation: inline

GAACAAAAACTCATCTCAGAAGAGGATCTG
type: SequenceAnnotation
persistent identity: annotation2428807
display id: annotation2428807
version: 1
top level: BBa_K1441002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1441002/annotation2428807/range2428807/1

https://synbiohub.org/public/igem/BBa_K1441002/annotation2428807/range2428807/1
type: Range
persistent identity: range2428807
display id: range2428807
version: 1
direction: direction/forward
top level: BBa_K1441002
start: 837
end: 866
orientation: inline

TTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTC
type: SequenceAnnotation
persistent identity: annotation2428805
display id: annotation2428805
version: 1
top level: BBa_K1441002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1441002/annotation2428805/range2428805/1

https://synbiohub.org/public/igem/BBa_K1441002/annotation2428805/range2428805/1
type: Range
persistent identity: range2428805
display id: range2428805
version: 1
direction: direction/forward
top level: BBa_K1441002
start: 7
end: 483
orientation: inline

GATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTTCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTT
type: SequenceAnnotation
persistent identity: annotation2428809
display id: annotation2428809
version: 1
top level: BBa_K1441002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1441002/annotation2428809/range2428809/1

https://synbiohub.org/public/igem/BBa_K1441002/annotation2428809/range2428809/1
type: Range
persistent identity: range2428809
display id: range2428809
version: 1
direction: direction/forward
top level: BBa_K1441002
start: 1239
end: 1650
orientation: inline

BBa_J72153
type: ComponentDefinition
persistent identity: BBa J72153
display id: BBa_J72153
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ampicillin + kanamycin resistant p15A vector (2ab vector)
creator: Josh Kittleson
created: 2012-08-11T11:00:00Z
modified: 2015-05-08T01:08:28Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part is referred to internally as pBjh1601AK.
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72153_sequence/1

pSB4A1
type: ComponentDefinition
persistent identity: pSB4A1
display id: pSB4A1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4A1 (Replaced by pSB4A5)
creator: Reshma Shetty
created: 2005-07-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_1_
m user id: 0
owner id: 25
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Suffix
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/pSB4A1_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation1782680
display id: annotation1782680
version: 1
top level: pSB4A1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/pSB4A1/annotation1782680/range1782680/1

https://synbiohub.org/public/igem/pSB4A1/annotation1782680/range1782680/1
type: Range
persistent identity: range1782680
display id: range1782680
version: 1
direction: direction/forward
top level: pSB4A1
start: 1
end: 21
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1782686
display id: annotation1782686
version: 1
top level: pSB4A1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/pSB4A1/annotation1782686/range1782686/1

https://synbiohub.org/public/igem/pSB4A1/annotation1782686/range1782686/1
type: Range
persistent identity: range1782686
display id: range1782686
version: 1
direction: direction/forward
top level: pSB4A1
start: 3033
end: 3054
orientation: inline

BBa_K801004
type: ComponentDefinition
persistent identity: BBa K801004
display id: BBa_K801004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM104 yeast shuttle vector with GAL1 promoter
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is based on the plasmid pTUM100 (BBa_K801000). The galactose-inducible GAL1 yeast promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
mutable notes: All forbidden restriction sites have been removed, making this plasmid RFC25-compatible.
mutable provenance: Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 2 ?? origin
sequence annotation: pUC1 origin
sequence annotation: CYC1 transcription terminator
sequence annotation: Ampicillin
sequence annotation: T7 promoter/priming site
sequence annotation: URA3
sequence annotation: f1 origin
sequence annotation: PGAL1
sequence: https://synbiohub.org/public/igem/BBa_K801004_sequence/1

2 ?? origin
type: SequenceAnnotation
persistent identity: annotation2213074
display id: annotation2213074
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213074/range2213074/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213074/range2213074/1
type: Range
persistent identity: range2213074
display id: range2213074
version: 1
direction: direction/forward
top level: BBa_K801004
start: 3281
end: 4752
orientation: inline

pUC1 origin
type: SequenceAnnotation
persistent identity: annotation2213071
display id: annotation2213071
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213071/range2213071/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213071/range2213071/1
type: Range
persistent identity: range2213071
display id: range2213071
version: 1
direction: direction/forward
top level: BBa_K801004
start: 474
end: 1147
orientation: inline

CYC1 transcription terminator
type: SequenceAnnotation
persistent identity: annotation2213070
display id: annotation2213070
version: 1
top level: BBa_K801004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213070/range2213070/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213070/range2213070/1
type: Range
persistent identity: range2213070
display id: range2213070
version: 1
direction: direction/forward
top level: BBa_K801004
start: 44
end: 292
orientation: inline

Ampicillin
type: SequenceAnnotation
persistent identity: annotation2213072
display id: annotation2213072
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213072/range2213072/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213072/range2213072/1
type: Range
persistent identity: range2213072
display id: range2213072
version: 1
direction: direction/forward
top level: BBa_K801004
start: 1292
end: 2152
orientation: inline

T7 promoter/priming site
type: SequenceAnnotation
persistent identity: annotation2213077
display id: annotation2213077
version: 1
top level: BBa_K801004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213077/range2213077/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213077/range2213077/1
type: Range
persistent identity: range2213077
display id: range2213077
version: 1
direction: direction/forward
top level: BBa_K801004
start: 5767
end: 5786
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2213073
display id: annotation2213073
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213073/range2213073/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213073/range2213073/1
type: Range
persistent identity: range2213073
display id: range2213073
version: 1
direction: direction/forward
top level: BBa_K801004
start: 2170
end: 3277
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2213075
display id: annotation2213075
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213075/range2213075/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213075/range2213075/1
type: Range
persistent identity: range2213075
display id: range2213075
version: 1
direction: direction/forward
top level: BBa_K801004
start: 4820
end: 5275
orientation: inline

PGAL1
type: SequenceAnnotation
persistent identity: annotation2213076
display id: annotation2213076
version: 1
top level: BBa_K801004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213076/range2213076/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213076/range2213076/1
type: Range
persistent identity: range2213076
display id: range2213076
version: 1
direction: direction/forward
top level: BBa_K801004
start: 5293
end: 5743
orientation: inline

BBa_J153000
type: ComponentDefinition
persistent identity: BBa J153000
display id: BBa_J153000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Broad-host-range shuttle vector pPMQAK1
creator: Daniel Camsund
created: 2012-02-02T12:00:00Z
modified: 2015-08-31T04:08:32Z
discontinued: false
dominant: false
group u list: _643_
m user id: 0
owner id: 1292
owning group id: 265
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The broad-host-range shuttle vector pPMQAK1 is a BioBrick-compatible plasmid that provides ampicillin and kanamycin/neomycin resistance (Genbank GU933126). As pPMQAK1 contains part of the pSB1AK3 plasmid it contains the same flanking terminators, and it is possible to use the VF2 (BBa_G00100) and VR (BBa_G00101) primers for colony PCR and sequencing. It was originally developed to allow for the use of BioBrick constructs in cyanobacteria (Huang etal 2010). But because of its RSF1010-derived broad-host-range replicon, it is expected to replicate successfully in most gram negative bacteria (Meyer 2009). Plasmids with a RSF1010-derived replicon, which belong to the IncQ group, have been found to have a copy number of 10 in Escherichia coli (Frey and Bagdasarian 1989), and from 10 (Marraccini etal 1993) to 30 (Ng etal 2000) per cell in the cyanobacterium Synechocystis sp. PCC 6803. Practical notes pPMQAK1 can be transformed into competent E. coli as preferred, but appears to give a lower number of transformants as compared to other cloning vectors (such as the pSB1***-series). The relatively low copy number also means that plasmid prep yields will be low. Further, it is often difficult to completely digest all plasmid, as judged by agarose gel electrophoresis. Conjugation (triparental mating) can be used to transfer pPMQAK1 into cyanobacteria as it carries the required mobilization genes and an oriT (origin of transfer, or bom-site) that enable conjugative transfer with the help of an additional conjugal plasmid. More info about engineering cyanobacteria, conjugation and detailed protocols can be found in the dedicated Methods in Enzymology chapter (Heidorn etal 2011). References Frey,J. and Bagdasarian,M. (1989) The molecular biology of IncQ plasmids. In Thomas,C.M. (ed.), Promiscuous Plasmids of Gram-negative Bacteria. Academic Press, London, pp. 79???94. Heidorn, T., Camsund, D., Huang, H.H., Lindberg, P., Oliveira, P., Stensjo, K. and Lindblad, P. Synthetic biology …
mutable notes: As cloning by plasmid preparation and subsequent digestion/ligation was problematic due to low copy number and difficulties in digesting the RSF1010-derived part from pAWG1.1, pPMQAK1 was made using PCR and subsequent digestion/ligation.
mutable provenance: pPMQAK1 was constructed by ligating a PCR product from the pAWG1.1 plasmid, corresponding to the RSF1010 replicon, with a PCR product from the pSB1AK3 plasmid, corresponding to the BioBrick cloning site with flanking functional sequences plus the ampicillin and kanamycin resistance cassettes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Suffix
sequence annotation: Prefix
sequence annotation: Terminator
sequence annotation: VF2 (BBa_G00100)
sequence annotation: repC
sequence annotation: mobB
sequence annotation: repA
sequence annotation: ampR
sequence annotation: mobA
sequence annotation: repressor protein F (predicted)
sequence annotation: mobC
sequence annotation: VR (BBa_G00101)
sequence annotation: unknown protein E (predicted)
sequence annotation: kanR
sequence annotation: oriV
sequence annotation: repB
sequence: https://synbiohub.org/public/igem/BBa_J153000_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2170031
display id: annotation2170031
version: 1
top level: BBa_J153000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170031/range2170031/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170031/range2170031/1
type: Range
persistent identity: range2170031
display id: range2170031
version: 1
direction: direction/forward
top level: BBa_J153000
start: 1
end: 21
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2170047
display id: annotation2170047
version: 1
top level: BBa_J153000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170047/range2170047/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170047/range2170047/1
type: Range
persistent identity: range2170047
display id: range2170047
version: 1
direction: direction/forward
top level: BBa_J153000
start: 7676
end: 7697
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2170046
display id: annotation2170046
version: 1
top level: BBa_J153000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170046/range2170046/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170046/range2170046/1
type: Range
persistent identity: range2170046
display id: range2170046
version: 1
direction: direction/reverse
top level: BBa_J153000
start: 7647
end: 7666
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2170032
display id: annotation2170032
version: 1
top level: BBa_J153000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170032/range2170032/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170032/range2170032/1
type: Range
persistent identity: range2170032
display id: range2170032
version: 1
direction: direction/forward
top level: BBa_J153000
start: 30
end: 62
orientation: inline

VF2 (BBa_G00100)
type: SequenceAnnotation
persistent identity: annotation2170045
display id: annotation2170045
version: 1
top level: BBa_J153000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170045/range2170045/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170045/range2170045/1
type: Range
persistent identity: range2170045
display id: range2170045
version: 1
direction: direction/forward
top level: BBa_J153000
start: 7557
end: 7577
orientation: inline

repC
type: SequenceAnnotation
persistent identity: annotation2170042
display id: annotation2170042
version: 1
top level: BBa_J153000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170042/range2170042/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170042/range2170042/1
type: Range
persistent identity: range2170042
display id: range2170042
version: 1
direction: direction/forward
top level: BBa_J153000
start: 4631
end: 5482
orientation: inline

mobB
type: SequenceAnnotation
persistent identity: annotation2170037
display id: annotation2170037
version: 1
top level: BBa_J153000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170037/range2170037/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170037/range2170037/1
type: Range
persistent identity: range2170037
display id: range2170037
version: 1
direction: direction/forward
top level: BBa_J153000
start: 1913
end: 2326
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2170041
display id: annotation2170041
version: 1
top level: BBa_J153000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170041/range2170041/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170041/range2170041/1
type: Range
persistent identity: range2170041
display id: range2170041
version: 1
direction: direction/forward
top level: BBa_J153000
start: 3805
end: 4644
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2170044
display id: annotation2170044
version: 1
top level: BBa_J153000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170044/range2170044/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170044/range2170044/1
type: Range
persistent identity: range2170044
display id: range2170044
version: 1
direction: direction/reverse
top level: BBa_J153000
start: 6575
end: 7435
orientation: inline

mobA
type: SequenceAnnotation
persistent identity: annotation2170036
display id: annotation2170036
version: 1
top level: BBa_J153000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170036/range2170036/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170036/range2170036/1
type: Range
persistent identity: range2170036
display id: range2170036
version: 1
direction: direction/forward
top level: BBa_J153000
start: 1165
end: 3294
orientation: inline

repressor protein F (predicted)
type: SequenceAnnotation
persistent identity: annotation2170040
display id: annotation2170040
version: 1
top level: BBa_J153000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170040/range2170040/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170040/range2170040/1
type: Range
persistent identity: range2170040
display id: range2170040
version: 1
direction: direction/forward
top level: BBa_J153000
start: 3569
end: 3775
orientation: inline

mobC
type: SequenceAnnotation
persistent identity: annotation2170035
display id: annotation2170035
version: 1
top level: BBa_J153000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170035/range2170035/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170035/range2170035/1
type: Range
persistent identity: range2170035
display id: range2170035
version: 1
direction: direction/reverse
top level: BBa_J153000
start: 682
end: 966
orientation: inline

VR (BBa_G00101)
type: SequenceAnnotation
persistent identity: annotation2170033
display id: annotation2170033
version: 1
top level: BBa_J153000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170033/range2170033/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170033/range2170033/1
type: Range
persistent identity: range2170033
display id: range2170033
version: 1
direction: direction/reverse
top level: BBa_J153000
start: 155
end: 174
orientation: inline

unknown protein E (predicted)
type: SequenceAnnotation
persistent identity: annotation2170039
display id: annotation2170039
version: 1
top level: BBa_J153000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170039/range2170039/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170039/range2170039/1
type: Range
persistent identity: range2170039
display id: range2170039
version: 1
direction: direction/forward
top level: BBa_J153000
start: 3355
end: 3567
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation2170043
display id: annotation2170043
version: 1
top level: BBa_J153000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170043/range2170043/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170043/range2170043/1
type: Range
persistent identity: range2170043
display id: range2170043
version: 1
direction: direction/reverse
top level: BBa_J153000
start: 5605
end: 6420
orientation: inline

oriV
type: SequenceAnnotation
persistent identity: annotation2170034
display id: annotation2170034
version: 1
top level: BBa_J153000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170034/range2170034/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170034/range2170034/1
type: Range
persistent identity: range2170034
display id: range2170034
version: 1
direction: direction/forward
top level: BBa_J153000
start: 265
end: 655
orientation: inline

repB
type: SequenceAnnotation
persistent identity: annotation2170038
display id: annotation2170038
version: 1
top level: BBa_J153000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J153000/annotation2170038/range2170038/1

https://synbiohub.org/public/igem/BBa_J153000/annotation2170038/range2170038/1
type: Range
persistent identity: range2170038
display id: range2170038
version: 1
direction: direction/forward
top level: BBa_J153000
start: 2323
end: 3294
orientation: inline

BBa_K494001
type: ComponentDefinition
persistent identity: BBa K494001
display id: BBa_K494001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Superior Screening Plasmid
creator: Haissi Cui, Tilman Flock, Sebastian Gude, Christoph Hartlm??ller, Florian Praetorius, Jan Sch????rmann, Tobi Wauer, Philipp Wortmann
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _573_
m user id: 0
owner id: 6997
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We designed a new screening systems based on the non-functional pSB1A10 plasmid. We improved its features for ??????in vivo?????? characterization of PoPS-based devices using fluorescent reporters . The plasmid still contains the Pbad arabinose-inducible induction system as a tunable input and eGFP as an internal standard for induction. However, we altered the reporter protein to mCherry. Furthermore we adjusted the BioBrick cloning site to allow cloning of additional parts independent from the Input/Output measurement. This screening plasmid is designed to be used with a second Arabinose inducible promoter BBa_I13453 which is not included in this part. The improved screening system is optimized for the evaluation of PoPS-based devices in fluorescence measurements. RFP which was known to contain an RNase restriction site was replaced by mCherry which combines good expression yield, short maturation times and an acceptable and well-characterized quantum yield. A unique challenge for the characterization of our switches is the expression of a corresponding signal independent of the input/output measurement. Thus, we moved the BioBrick cloning site resulting in the fluorescent reporter being inside the cloning site and giving the possibility to clone independent parts behind the reporter protein. To fully function our screening plasmid need the arabinose inducible promoter BBa_I13453 in front of the PoPS-based device to screen. Using a second arabinose inducible promoter, we were able to keep eGFP as an internal standard for the tunable input via the Pbad arabinose-inducible induction system. The two identical promoters ensure the same rate of induction for eGFP and the tested PoPS-based device. Thus, obtaining comparable screening results is easy. Unfortunately, this design implicates a minor disadvantage. Two cloning steps are needed to gain an functional construct for testing any PoPS-based device.
mutable notes: PoPS-based devices can be cloned into plasmid in front of the second reporter gene, whereas another independent sequence, like a signal, can be inserted behind the reporter genes.
mutable provenance: Derived from pSB1A10
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Suffix
sequence annotation: eGFP E0040
sequence annotation: Prefix
sequence annotation: pBAD I10500
sequence: https://synbiohub.org/public/igem/BBa_K494001_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2112248
display id: annotation2112248
version: 1
top level: BBa_K494001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K494001/annotation2112248/range2112248/1

https://synbiohub.org/public/igem/BBa_K494001/annotation2112248/range2112248/1
type: Range
persistent identity: range2112248
display id: range2112248
version: 1
direction: direction/forward
top level: BBa_K494001
start: 1
end: 22
orientation: inline

eGFP E0040
type: SequenceAnnotation
persistent identity: annotation2112250
display id: annotation2112250
version: 1
top level: BBa_K494001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K494001/annotation2112250/range2112250/1

https://synbiohub.org/public/igem/BBa_K494001/annotation2112250/range2112250/1
type: Range
persistent identity: range2112250
display id: range2112250
version: 1
direction: direction/forward
top level: BBa_K494001
start: 3728
end: 4448
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2112251
display id: annotation2112251
version: 1
top level: BBa_K494001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K494001/annotation2112251/range2112251/1

https://synbiohub.org/public/igem/BBa_K494001/annotation2112251/range2112251/1
type: Range
persistent identity: range2112251
display id: range2112251
version: 1
direction: direction/forward
top level: BBa_K494001
start: 4501
end: 4516
orientation: inline

pBAD I10500
type: SequenceAnnotation
persistent identity: annotation2112249
display id: annotation2112249
version: 1
top level: BBa_K494001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K494001/annotation2112249/range2112249/1

https://synbiohub.org/public/igem/BBa_K494001/annotation2112249/range2112249/1
type: Range
persistent identity: range2112249
display id: range2112249
version: 1
direction: direction/forward
top level: BBa_K494001
start: 2492
end: 3702
orientation: inline

BBa_J176126
type: ComponentDefinition
persistent identity: BBa J176126
display id: BBa_J176126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: V0201
creator: Brady Laughlin
created: 2011-10-22T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: VP64-NLS-pcDNA3.1+, pcDNA3.1+ with Eco/Spe NLS BioBrick inserted into Eco/Xba; amp, neo
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: bglobinsulator
sequence annotation: bla (ampicilin resistance)
sequence annotation: 3t3 forward primer 2.0 hopefully better
sequence annotation: SV40 early polyadelylation signal
sequence annotation: Hygromycin resistance gene
sequence annotation: BGH polyadenylation signal
sequence annotation: bla promoter
sequence annotation: possible biobrickable terminator with polyA signal terminator
sequence annotation: SpeI - XbaI ligation
sequence annotation: 3t3 forward primer bind
sequence annotation: Digestion product of ApaI and HindIII building bloc variation
sequence annotation: micro MCS site for biobrick inclusion
sequence annotation: FRT site
sequence annotation: pUC origin
sequence annotation: V5 epitope tagging
sequence annotation: stop codon?
sequence annotation: digestion overhangs
sequence annotation: BGH reverse priming site
sequence: https://synbiohub.org/public/igem/BBa_J176126_sequence/1

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160395
display id: annotation2160395
version: 1
top level: BBa_J176126
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160395/range2160395/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160395/range2160395/1
type: Range
persistent identity: range2160395
display id: range2160395
version: 1
direction: direction/forward
top level: BBa_J176126
start: 65
end: 404
orientation: inline

bla (ampicilin resistance)
type: SequenceAnnotation
persistent identity: annotation2160381
display id: annotation2160381
version: 1
top level: BBa_J176126
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160381/range2160381/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160381/range2160381/1
type: Range
persistent identity: range2160381
display id: range2160381
version: 1
direction: direction/reverse
top level: BBa_J176126
start: 4196
end: 5056
orientation: inline

3t3 forward primer 2.0 hopefully better
type: SequenceAnnotation
persistent identity: annotation2160391
display id: annotation2160391
version: 1
top level: BBa_J176126
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160391/range2160391/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160391/range2160391/1
type: Range
persistent identity: range2160391
display id: range2160391
version: 1
direction: direction/forward
top level: BBa_J176126
start: 920
end: 943
orientation: inline

SV40 early polyadelylation signal
type: SequenceAnnotation
persistent identity: annotation2160379
display id: annotation2160379
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160379/range2160379/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160379/range2160379/1
type: Range
persistent identity: range2160379
display id: range2160379
version: 1
direction: direction/forward
top level: BBa_J176126
start: 2865
end: 2995
orientation: inline

Hygromycin resistance gene
type: SequenceAnnotation
persistent identity: annotation2160378
display id: annotation2160378
version: 1
top level: BBa_J176126
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160378/range2160378/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160378/range2160378/1
type: Range
persistent identity: range2160378
display id: range2160378
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1713
end: 2705
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160411
display id: annotation2160411
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160411/range2160411/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160411/range2160411/1
type: Range
persistent identity: range2160411
display id: range2160411
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1
end: 5157
orientation: inline

BGH polyadenylation signal
type: SequenceAnnotation
persistent identity: annotation2160376
display id: annotation2160376
version: 1
top level: BBa_J176126
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160376/range2160376/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160376/range2160376/1
type: Range
persistent identity: range2160376
display id: range2160376
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1151
end: 1375
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160400
display id: annotation2160400
version: 1
top level: BBa_J176126
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160400/range2160400/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160400/range2160400/1
type: Range
persistent identity: range2160400
display id: range2160400
version: 1
direction: direction/forward
top level: BBa_J176126
start: 411
end: 425
orientation: inline

bla promoter
type: SequenceAnnotation
persistent identity: annotation2160382
display id: annotation2160382
version: 1
top level: BBa_J176126
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160382/range2160382/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160382/range2160382/1
type: Range
persistent identity: range2160382
display id: range2160382
version: 1
direction: direction/reverse
top level: BBa_J176126
start: 5057
end: 5155
orientation: inline

possible biobrickable terminator with polyA signal terminator
type: SequenceAnnotation
persistent identity: annotation2160392
display id: annotation2160392
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160392/range2160392/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160392/range2160392/1
type: Range
persistent identity: range2160392
display id: range2160392
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1117
end: 1375
orientation: inline

possible biobrickable terminator with polyA signal terminator
type: SequenceAnnotation
persistent identity: annotation2160393
display id: annotation2160393
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160393/range2160393/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160393/range2160393/1
type: Range
persistent identity: range2160393
display id: range2160393
version: 1
direction: direction/forward
top level: BBa_J176126
start: 763
end: 767
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160396
display id: annotation2160396
version: 1
top level: BBa_J176126
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160396/range2160396/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160396/range2160396/1
type: Range
persistent identity: range2160396
display id: range2160396
version: 1
direction: direction/reverse
top level: BBa_J176126
start: 321
end: 340
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160394
display id: annotation2160394
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160394/range2160394/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160394/range2160394/1
type: Range
persistent identity: range2160394
display id: range2160394
version: 1
direction: direction/forward
top level: BBa_J176126
start: 331
end: 331
orientation: inline

SpeI - XbaI ligation
type: SequenceAnnotation
persistent identity: annotation2160388
display id: annotation2160388
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160388/range2160388/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160388/range2160388/1
type: Range
persistent identity: range2160388
display id: range2160388
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1045
end: 1047
orientation: inline

3t3 forward primer bind
type: SequenceAnnotation
persistent identity: annotation2160390
display id: annotation2160390
version: 1
top level: BBa_J176126
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160390/range2160390/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160390/range2160390/1
type: Range
persistent identity: range2160390
display id: range2160390
version: 1
direction: direction/forward
top level: BBa_J176126
start: 906
end: 930
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160399
display id: annotation2160399
version: 1
top level: BBa_J176126
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160399/range2160399/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160399/range2160399/1
type: Range
persistent identity: range2160399
display id: range2160399
version: 1
direction: direction/forward
top level: BBa_J176126
start: 411
end: 750
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160401
display id: annotation2160401
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160401/range2160401/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160401/range2160401/1
type: Range
persistent identity: range2160401
display id: range2160401
version: 1
direction: direction/forward
top level: BBa_J176126
start: 405
end: 410
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160402
display id: annotation2160402
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160402/range2160402/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160402/range2160402/1
type: Range
persistent identity: range2160402
display id: range2160402
version: 1
direction: direction/forward
top level: BBa_J176126
start: 677
end: 677
orientation: inline

Digestion product of ApaI and HindIII building bloc variation
type: SequenceAnnotation
persistent identity: annotation2160383
display id: annotation2160383
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160383/range2160383/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160383/range2160383/1
type: Range
persistent identity: range2160383
display id: range2160383
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1020
end: 5157
orientation: inline

micro MCS site for biobrick inclusion
type: SequenceAnnotation
persistent identity: annotation2160387
display id: annotation2160387
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160387/range2160387/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160387/range2160387/1
type: Range
persistent identity: range2160387
display id: range2160387
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1020
end: 1050
orientation: inline

SpeI - XbaI ligation
type: SequenceAnnotation
persistent identity: annotation2160389
display id: annotation2160389
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160389/range2160389/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160389/range2160389/1
type: Range
persistent identity: range2160389
display id: range2160389
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1048
end: 1048
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160405
display id: annotation2160405
version: 1
top level: BBa_J176126
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160405/range2160405/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160405/range2160405/1
type: Range
persistent identity: range2160405
display id: range2160405
version: 1
direction: direction/reverse
top level: BBa_J176126
start: 736
end: 750
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160409
display id: annotation2160409
version: 1
top level: BBa_J176126
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160409/range2160409/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160409/range2160409/1
type: Range
persistent identity: range2160409
display id: range2160409
version: 1
direction: direction/forward
top level: BBa_J176126
start: 60
end: 74
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160398
display id: annotation2160398
version: 1
top level: BBa_J176126
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160398/range2160398/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160398/range2160398/1
type: Range
persistent identity: range2160398
display id: range2160398
version: 1
direction: direction/reverse
top level: BBa_J176126
start: 390
end: 404
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160407
display id: annotation2160407
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160407/range2160407/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160407/range2160407/1
type: Range
persistent identity: range2160407
display id: range2160407
version: 1
direction: direction/forward
top level: BBa_J176126
start: 47
end: 51
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2160377
display id: annotation2160377
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160377/range2160377/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160377/range2160377/1
type: Range
persistent identity: range2160377
display id: range2160377
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1658
end: 1695
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2160380
display id: annotation2160380
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160380/range2160380/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160380/range2160380/1
type: Range
persistent identity: range2160380
display id: range2160380
version: 1
direction: direction/forward
top level: BBa_J176126
start: 3378
end: 4051
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160397
display id: annotation2160397
version: 1
top level: BBa_J176126
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160397/range2160397/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160397/range2160397/1
type: Range
persistent identity: range2160397
display id: range2160397
version: 1
direction: direction/forward
top level: BBa_J176126
start: 321
end: 340
orientation: inline

V5 epitope tagging
type: SequenceAnnotation
persistent identity: annotation2160374
display id: annotation2160374
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160374/range2160374/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160374/range2160374/1
type: Range
persistent identity: range2160374
display id: range2160374
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1066
end: 1107
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160404
display id: annotation2160404
version: 1
top level: BBa_J176126
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160404/range2160404/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160404/range2160404/1
type: Range
persistent identity: range2160404
display id: range2160404
version: 1
direction: direction/reverse
top level: BBa_J176126
start: 667
end: 686
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160403
display id: annotation2160403
version: 1
top level: BBa_J176126
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160403/range2160403/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160403/range2160403/1
type: Range
persistent identity: range2160403
display id: range2160403
version: 1
direction: direction/forward
top level: BBa_J176126
start: 667
end: 686
orientation: inline

Digestion product of ApaI and HindIII building bloc variation
type: SequenceAnnotation
persistent identity: annotation2160384
display id: annotation2160384
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160384/range2160384/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160384/range2160384/1
type: Range
persistent identity: range2160384
display id: range2160384
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1
end: 4
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160406
display id: annotation2160406
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160406/range2160406/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160406/range2160406/1
type: Range
persistent identity: range2160406
display id: range2160406
version: 1
direction: direction/forward
top level: BBa_J176126
start: 52
end: 54
orientation: inline

stop codon?
type: SequenceAnnotation
persistent identity: annotation2160386
display id: annotation2160386
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160386/range2160386/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160386/range2160386/1
type: Range
persistent identity: range2160386
display id: range2160386
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1129
end: 1131
orientation: inline

digestion overhangs
type: SequenceAnnotation
persistent identity: annotation2160385
display id: annotation2160385
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160385/range2160385/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160385/range2160385/1
type: Range
persistent identity: range2160385
display id: range2160385
version: 1
direction: direction/forward
top level: BBa_J176126
start: 1051
end: 1054
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160410
display id: annotation2160410
version: 1
top level: BBa_J176126
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160410/range2160410/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160410/range2160410/1
type: Range
persistent identity: range2160410
display id: range2160410
version: 1
direction: direction/forward
top level: BBa_J176126
start: 64
end: 64
orientation: inline

bglobinsulator
type: SequenceAnnotation
persistent identity: annotation2160408
display id: annotation2160408
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160408/range2160408/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160408/range2160408/1
type: Range
persistent identity: range2160408
display id: range2160408
version: 1
direction: direction/forward
top level: BBa_J176126
start: 760
end: 762
orientation: inline

BGH reverse priming site
type: SequenceAnnotation
persistent identity: annotation2160375
display id: annotation2160375
version: 1
top level: BBa_J176126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176126/annotation2160375/range2160375/1

https://synbiohub.org/public/igem/BBa_J176126/annotation2160375/range2160375/1
type: Range
persistent identity: range2160375
display id: range2160375
version: 1
direction: direction/reverse
top level: BBa_J176126
start: 1145
end: 1162
orientation: inline

BBa_K260004
type: ComponentDefinition
persistent identity: BBa K260004
display id: BBa_K260004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMA-@CC2FosMB-00500bp
creator: Kaj Bernhardt
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _356_
m user id: 0
owner id: 1072
owning group id: 57
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This plasmid backbone is based on pMA from Geneart. It has BioBrick prefix and suffix flanked on both sides by 50 bp of homology arms for insertion of the contained BioBrick into the 00500 locus of pCC2FosMB (BBa_K260001) by recombineering. The pMA-@CC2FosMB-00500bp backbone is available with BBa_K260015, which is composed of promoter-FRT-dhfr. If the PvuII fragment from BBa_K260004-BBa_K260015 is transferred by recombineering to the the 00500 locus of BBa_K260001-BBa_K260016, the intervening sequence between the FRT sites of BBa_K260015 and BBa_K260016 on the BBa_K260001 backbone will be 500 bp exactly. These plasmid backbones are similar and all allow recombineering-mediated transfer of the contained BioBrick (typically BBa_K260015) to the pCC2FosMB backbone BBa_K2600001. They differ in the homology arms, which determines the locus of insertion into pCC2FosMB. The bp numbers denote final inter-FRT distance as described above: BBa_K260004: pMA-CC2FosMB-00500bp BBa_K260005: pMA-CC2FosMB-01000bp BBa_K260006: pMA-CC2FosMB-02000bp BBa_K260007: pMA-CC2FosMB-05000bp BBa_K260008: pMA-RQ-CC2FosMB-10000bp
mutable notes: The 50 bp homologies were designed to delete 100 bp of BBa_K260001-BBa_K260016 and put the FRT site of BBa_K260015 (if used with the present backbone BBa_K260004) at a defined distance from the FRT site of BBa_K260016.
mutable provenance: Geneart plasmid pMA, with 2x 50 bp from pCC2FosMB K260001.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BBa_K260001 (pCC2FosMB) 5' homology FRT-00500bp
sequence annotation: BBa_K260001 (pCC2FosMB) 3' homology FRT-00500bp
sequence annotation: PvuII site
sequence annotation: prefix
sequence annotation: ColE1 origin
sequence annotation: suffix
sequence annotation: AmpR
sequence: https://synbiohub.org/public/igem/BBa_K260004_sequence/1

BBa_K260001 (pCC2FosMB) 5' homology FRT-00500bp
type: SequenceAnnotation
persistent identity: annotation2049440
display id: annotation2049440
version: 1
top level: BBa_K260004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260004/annotation2049440/range2049440/1

https://synbiohub.org/public/igem/BBa_K260004/annotation2049440/range2049440/1
type: Range
persistent identity: range2049440
display id: range2049440
version: 1
direction: direction/forward
top level: BBa_K260004
start: 2474
end: 2543
orientation: inline

BBa_K260001 (pCC2FosMB) 3' homology FRT-00500bp
type: SequenceAnnotation
persistent identity: annotation2049414
display id: annotation2049414
version: 1
top level: BBa_K260004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260004/annotation2049414/range2049414/1

https://synbiohub.org/public/igem/BBa_K260004/annotation2049414/range2049414/1
type: Range
persistent identity: range2049414
display id: range2049414
version: 1
direction: direction/forward
top level: BBa_K260004
start: 22
end: 91
orientation: inline

PvuII site
type: SequenceAnnotation
persistent identity: annotation2049421
display id: annotation2049421
version: 1
top level: BBa_K260004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K260004/annotation2049421/range2049421/1

https://synbiohub.org/public/igem/BBa_K260004/annotation2049421/range2049421/1
type: Range
persistent identity: range2049421
display id: range2049421
version: 1
direction: direction/forward
top level: BBa_K260004
start: 2468
end: 2473
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2049445
display id: annotation2049445
version: 1
top level: BBa_K260004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260004/annotation2049445/range2049445/1

https://synbiohub.org/public/igem/BBa_K260004/annotation2049445/range2049445/1
type: Range
persistent identity: range2049445
display id: range2049445
version: 1
direction: direction/forward
top level: BBa_K260004
start: 2544
end: 2565
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation2049436
display id: annotation2049436
version: 1
top level: BBa_K260004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260004/annotation2049436/range2049436/1

https://synbiohub.org/public/igem/BBa_K260004/annotation2049436/range2049436/1
type: Range
persistent identity: range2049436
display id: range2049436
version: 1
direction: direction/forward
top level: BBa_K260004
start: 1617
end: 2284
orientation: inline

PvuII site
type: SequenceAnnotation
persistent identity: annotation2049420
display id: annotation2049420
version: 1
top level: BBa_K260004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K260004/annotation2049420/range2049420/1

https://synbiohub.org/public/igem/BBa_K260004/annotation2049420/range2049420/1
type: Range
persistent identity: range2049420
display id: range2049420
version: 1
direction: direction/forward
top level: BBa_K260004
start: 92
end: 97
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2049410
display id: annotation2049410
version: 1
top level: BBa_K260004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260004/annotation2049410/range2049410/1

https://synbiohub.org/public/igem/BBa_K260004/annotation2049410/range2049410/1
type: Range
persistent identity: range2049410
display id: range2049410
version: 1
direction: direction/forward
top level: BBa_K260004
start: 1
end: 21
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2049431
display id: annotation2049431
version: 1
top level: BBa_K260004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260004/annotation2049431/range2049431/1

https://synbiohub.org/public/igem/BBa_K260004/annotation2049431/range2049431/1
type: Range
persistent identity: range2049431
display id: range2049431
version: 1
direction: direction/forward
top level: BBa_K260004
start: 609
end: 1469
orientation: inline

pSB2K4
type: ComponentDefinition
persistent identity: pSB2K4
display id: pSB2K4
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB2K3-derived BioBrick plasmid free of many restriction sites
creator: Austin Che, Leon Chan and Sri Kosuri
created: 2006-01-19T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 22
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Derived from pSB2K3 with many restriction enzyme sites removed by site-directed mutagenesis (~2 dozen mutations). Plasmid is useful when there is a need to cut with a variety of different restriction enzymes. Most symmetric cutters have been removed, and some non-symmetric cutters have also including AarI, AcuI, BbsI, BbvCI, BciVI, BsaI, BsmBI, EarI, N.BstNBI, and SgrAI. Works in exactly same way as pSB2K3. See <bb_part>pSB2K3</bb_part> for more information. Note that the VF2 site has a single base mutation in the middle of it. However, VF2 has been used successfully as a primer for PCR with this plasmid.
mutable provenance: pSB2K3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: repE binding site
sequence annotation: oriS
sequence annotation: Possible -35
sequence annotation: BioBrick prefix
sequence annotation: Possible -10
sequence annotation: Predicted transcription start
sequence annotation: Stem loop
sequence annotation: T->G
sequence annotation: end of terminator
sequence annotation: f1 ori
sequence annotation: Verification reverse
sequence annotation: T->C
sequence annotation: repE
sequence annotation: C->T
sequence annotation: VF2 (not an exact match, but works)
sequence annotation: nnpp promoter 2 (0.96)'
sequence annotation: Low GC region
sequence annotation: Maybe A or G
sequence annotation: repL
sequence annotation: incC
sequence annotation: BioBrick suffix
sequence annotation: G->A
sequence annotation: G->C
sequence annotation: nnpp promoter 1 (.92)'
sequence annotation: Predicted -35
sequence annotation: E. coli his operon terminator
sequence annotation: nnpp promoter 3 (0.95)'
sequence annotation: T->A mutation
sequence annotation: A->G mutation
sequence annotation: A->C
sequence annotation: Predicted -10
sequence annotation: C->A

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974325
display id: annotation1974325
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974325/range1974325/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974325/range1974325/1
type: Range
persistent identity: range1974325
display id: range1974325
version: 1
direction: direction/forward
top level: pSB2K4
start: 1527
end: 1537
orientation: inline

oriS
type: SequenceAnnotation
persistent identity: annotation1974328
display id: annotation1974328
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974328/range1974328/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974328/range1974328/1
type: Range
persistent identity: range1974328
display id: range1974328
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1490
end: 1706
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974323
display id: annotation1974323
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974323/range1974323/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974323/range1974323/1
type: Range
persistent identity: range1974323
display id: range1974323
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1477
end: 1482
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1974355
display id: annotation1974355
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974355/range1974355/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974355/range1974355/1
type: Range
persistent identity: range1974355
display id: range1974355
version: 1
direction: direction/forward
top level: pSB2K4
start: 4446
end: 4467
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974320
display id: annotation1974320
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974320/range1974320/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974320/range1974320/1
type: Range
persistent identity: range1974320
display id: range1974320
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1455
end: 1463
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974318
display id: annotation1974318
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974318/range1974318/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974318/range1974318/1
type: Range
persistent identity: range1974318
display id: range1974318
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1447
end: 1447
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1974354
display id: annotation1974354
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974354/range1974354/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974354/range1974354/1
type: Range
persistent identity: range1974354
display id: range1974354
version: 1
direction: direction/forward
top level: pSB2K4
start: 4402
end: 4421
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974334
display id: annotation1974334
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974334/range1974334/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974334/range1974334/1
type: Range
persistent identity: range1974334
display id: range1974334
version: 1
direction: direction/forward
top level: pSB2K4
start: 2699
end: 2699
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1974353
display id: annotation1974353
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974353/range1974353/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974353/range1974353/1
type: Range
persistent identity: range1974353
display id: range1974353
version: 1
direction: direction/forward
top level: pSB2K4
start: 4389
end: 4392
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974315
display id: annotation1974315
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974315/range1974315/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974315/range1974315/1
type: Range
persistent identity: range1974315
display id: range1974315
version: 1
direction: direction/forward
top level: pSB2K4
start: 1426
end: 1431
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation1974297
display id: annotation1974297
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974297/range1974297/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974297/range1974297/1
type: Range
persistent identity: range1974297
display id: range1974297
version: 1
direction: direction/reverse
top level: pSB2K4
start: 269
end: 317
orientation: inline

Verification reverse
type: SequenceAnnotation
persistent identity: annotation1974296
display id: annotation1974296
version: 1
top level: pSB2K4
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974296/range1974296/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974296/range1974296/1
type: Range
persistent identity: range1974296
display id: range1974296
version: 1
direction: direction/reverse
top level: pSB2K4
start: 184
end: 203
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974338
display id: annotation1974338
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974338/range1974338/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974338/range1974338/1
type: Range
persistent identity: range1974338
display id: range1974338
version: 1
direction: direction/forward
top level: pSB2K4
start: 3251
end: 3251
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974349
display id: annotation1974349
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974349/range1974349/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974349/range1974349/1
type: Range
persistent identity: range1974349
display id: range1974349
version: 1
direction: direction/forward
top level: pSB2K4
start: 3903
end: 3903
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974304
display id: annotation1974304
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974304/range1974304/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974304/range1974304/1
type: Range
persistent identity: range1974304
display id: range1974304
version: 1
direction: direction/reverse
top level: pSB2K4
start: 568
end: 586
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974307
display id: annotation1974307
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974307/range1974307/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974307/range1974307/1
type: Range
persistent identity: range1974307
display id: range1974307
version: 1
direction: direction/reverse
top level: pSB2K4
start: 623
end: 641
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974314
display id: annotation1974314
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974314/range1974314/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974314/range1974314/1
type: Range
persistent identity: range1974314
display id: range1974314
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1426
end: 1426
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1974310
display id: annotation1974310
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974310/range1974310/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974310/range1974310/1
type: Range
persistent identity: range1974310
display id: range1974310
version: 1
direction: direction/reverse
top level: pSB2K4
start: 644
end: 1399
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974343
display id: annotation1974343
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974343/range1974343/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974343/range1974343/1
type: Range
persistent identity: range1974343
display id: range1974343
version: 1
direction: direction/forward
top level: pSB2K4
start: 3394
end: 3394
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974306
display id: annotation1974306
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974306/range1974306/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974306/range1974306/1
type: Range
persistent identity: range1974306
display id: range1974306
version: 1
direction: direction/reverse
top level: pSB2K4
start: 597
end: 619
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1974350
display id: annotation1974350
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974350/range1974350/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974350/range1974350/1
type: Range
persistent identity: range1974350
display id: range1974350
version: 1
direction: direction/forward
top level: pSB2K4
start: 4119
end: 4119
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974317
display id: annotation1974317
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974317/range1974317/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974317/range1974317/1
type: Range
persistent identity: range1974317
display id: range1974317
version: 1
direction: direction/forward
top level: pSB2K4
start: 1444
end: 1452
orientation: inline

VF2 (not an exact match, but works)
type: SequenceAnnotation
persistent identity: annotation1974351
display id: annotation1974351
version: 1
top level: pSB2K4
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974351/range1974351/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974351/range1974351/1
type: Range
persistent identity: range1974351
display id: range1974351
version: 1
direction: direction/forward
top level: pSB2K4
start: 4313
end: 4332
orientation: inline

nnpp promoter 2 (0.96)'
type: SequenceAnnotation
persistent identity: annotation1974341
display id: annotation1974341
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974341/range1974341/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974341/range1974341/1
type: Range
persistent identity: range1974341
display id: range1974341
version: 1
direction: direction/forward
top level: pSB2K4
start: 3353
end: 3398
orientation: inline

Low GC region
type: SequenceAnnotation
persistent identity: annotation1974330
display id: annotation1974330
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974330/range1974330/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974330/range1974330/1
type: Range
persistent identity: range1974330
display id: range1974330
version: 1
direction: direction/forward
top level: pSB2K4
start: 1823
end: 1844
orientation: inline

Maybe A or G
type: SequenceAnnotation
persistent identity: annotation1974332
display id: annotation1974332
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974332/range1974332/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974332/range1974332/1
type: Range
persistent identity: range1974332
display id: range1974332
version: 1
direction: direction/forward
top level: pSB2K4
start: 2565
end: 2565
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974316
display id: annotation1974316
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974316/range1974316/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974316/range1974316/1
type: Range
persistent identity: range1974316
display id: range1974316
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1433
end: 1441
orientation: inline

repL
type: SequenceAnnotation
persistent identity: annotation1974335
display id: annotation1974335
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974335/range1974335/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974335/range1974335/1
type: Range
persistent identity: range1974335
display id: range1974335
version: 1
direction: direction/forward
top level: pSB2K4
start: 2316
end: 3161
orientation: inline

incC
type: SequenceAnnotation
persistent identity: annotation1974302
display id: annotation1974302
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974302/range1974302/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974302/range1974302/1
type: Range
persistent identity: range1974302
display id: range1974302
version: 1
direction: direction/reverse
top level: pSB2K4
start: 394
end: 641
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1974291
display id: annotation1974291
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974291/range1974291/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974291/range1974291/1
type: Range
persistent identity: range1974291
display id: range1974291
version: 1
direction: direction/forward
top level: pSB2K4
start: 1
end: 21
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974305
display id: annotation1974305
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974305/range1974305/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974305/range1974305/1
type: Range
persistent identity: range1974305
display id: range1974305
version: 1
direction: direction/forward
top level: pSB2K4
start: 578
end: 578
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation1974337
display id: annotation1974337
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974337/range1974337/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974337/range1974337/1
type: Range
persistent identity: range1974337
display id: range1974337
version: 1
direction: direction/forward
top level: pSB2K4
start: 3017
end: 3017
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974336
display id: annotation1974336
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974336/range1974336/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974336/range1974336/1
type: Range
persistent identity: range1974336
display id: range1974336
version: 1
direction: direction/forward
top level: pSB2K4
start: 2774
end: 2774
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974326
display id: annotation1974326
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974326/range1974326/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974326/range1974326/1
type: Range
persistent identity: range1974326
display id: range1974326
version: 1
direction: direction/forward
top level: pSB2K4
start: 1552
end: 1570
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1974313
display id: annotation1974313
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974313/range1974313/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974313/range1974313/1
type: Range
persistent identity: range1974313
display id: range1974313
version: 1
direction: direction/forward
top level: pSB2K4
start: 1142
end: 1142
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974301
display id: annotation1974301
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974301/range1974301/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974301/range1974301/1
type: Range
persistent identity: range1974301
display id: range1974301
version: 1
direction: direction/reverse
top level: pSB2K4
start: 477
end: 495
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974347
display id: annotation1974347
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974347/range1974347/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974347/range1974347/1
type: Range
persistent identity: range1974347
display id: range1974347
version: 1
direction: direction/forward
top level: pSB2K4
start: 3849
end: 3849
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974322
display id: annotation1974322
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974322/range1974322/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974322/range1974322/1
type: Range
persistent identity: range1974322
display id: range1974322
version: 1
direction: direction/forward
top level: pSB2K4
start: 1460
end: 1460
orientation: inline

nnpp promoter 1 (.92)'
type: SequenceAnnotation
persistent identity: annotation1974344
display id: annotation1974344
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974344/range1974344/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974344/range1974344/1
type: Range
persistent identity: range1974344
display id: range1974344
version: 1
direction: direction/forward
top level: pSB2K4
start: 3375
end: 3420
orientation: inline

Predicted -35
type: SequenceAnnotation
persistent identity: annotation1974340
display id: annotation1974340
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974340/range1974340/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974340/range1974340/1
type: Range
persistent identity: range1974340
display id: range1974340
version: 1
direction: direction/forward
top level: pSB2K4
start: 3359
end: 3364
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1974292
display id: annotation1974292
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974292/range1974292/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974292/range1974292/1
type: Range
persistent identity: range1974292
display id: range1974292
version: 1
direction: direction/forward
top level: pSB2K4
start: 51
end: 120
orientation: inline

nnpp promoter 3 (0.95)'
type: SequenceAnnotation
persistent identity: annotation1974339
display id: annotation1974339
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974339/range1974339/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974339/range1974339/1
type: Range
persistent identity: range1974339
display id: range1974339
version: 1
direction: direction/forward
top level: pSB2K4
start: 3269
end: 3314
orientation: inline

T->A mutation
type: SequenceAnnotation
persistent identity: annotation1974303
display id: annotation1974303
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974303/range1974303/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974303/range1974303/1
type: Range
persistent identity: range1974303
display id: range1974303
version: 1
direction: direction/forward
top level: pSB2K4
start: 551
end: 551
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1974293
display id: annotation1974293
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974293/range1974293/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974293/range1974293/1
type: Range
persistent identity: range1974293
display id: range1974293
version: 1
direction: direction/forward
top level: pSB2K4
start: 59
end: 91
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974346
display id: annotation1974346
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974346/range1974346/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974346/range1974346/1
type: Range
persistent identity: range1974346
display id: range1974346
version: 1
direction: direction/forward
top level: pSB2K4
start: 3702
end: 3702
orientation: inline

A->G mutation
type: SequenceAnnotation
persistent identity: annotation1974300
display id: annotation1974300
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974300/range1974300/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974300/range1974300/1
type: Range
persistent identity: range1974300
display id: range1974300
version: 1
direction: direction/forward
top level: pSB2K4
start: 462
end: 462
orientation: inline

Low GC region
type: SequenceAnnotation
persistent identity: annotation1974329
display id: annotation1974329
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974329/range1974329/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974329/range1974329/1
type: Range
persistent identity: range1974329
display id: range1974329
version: 1
direction: direction/forward
top level: pSB2K4
start: 1636
end: 1682
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974333
display id: annotation1974333
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974333/range1974333/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974333/range1974333/1
type: Range
persistent identity: range1974333
display id: range1974333
version: 1
direction: direction/forward
top level: pSB2K4
start: 2567
end: 2567
orientation: inline

A->C
type: SequenceAnnotation
persistent identity: annotation1974308
display id: annotation1974308
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974308/range1974308/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974308/range1974308/1
type: Range
persistent identity: range1974308
display id: range1974308
version: 1
direction: direction/forward
top level: pSB2K4
start: 692
end: 692
orientation: inline

Predicted -10
type: SequenceAnnotation
persistent identity: annotation1974342
display id: annotation1974342
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974342/range1974342/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974342/range1974342/1
type: Range
persistent identity: range1974342
display id: range1974342
version: 1
direction: direction/forward
top level: pSB2K4
start: 3379
end: 3387
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974352
display id: annotation1974352
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974352/range1974352/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974352/range1974352/1
type: Range
persistent identity: range1974352
display id: range1974352
version: 1
direction: direction/forward
top level: pSB2K4
start: 4321
end: 4321
orientation: inline

C->A
type: SequenceAnnotation
persistent identity: annotation1974295
display id: annotation1974295
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974295/range1974295/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974295/range1974295/1
type: Range
persistent identity: range1974295
display id: range1974295
version: 1
direction: direction/forward
top level: pSB2K4
start: 146
end: 146
orientation: inline

G->T
type: SequenceAnnotation
persistent identity: annotation1974319
display id: annotation1974319
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974319/range1974319/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974319/range1974319/1
type: Range
persistent identity: range1974319
display id: range1974319
version: 1
direction: direction/forward
top level: pSB2K4
start: 1454
end: 1454
orientation: inline

T->A
type: SequenceAnnotation
persistent identity: annotation1974345
display id: annotation1974345
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974345/range1974345/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974345/range1974345/1
type: Range
persistent identity: range1974345
display id: range1974345
version: 1
direction: direction/forward
top level: pSB2K4
start: 3507
end: 3507
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974309
display id: annotation1974309
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974309/range1974309/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974309/range1974309/1
type: Range
persistent identity: range1974309
display id: range1974309
version: 1
direction: direction/forward
top level: pSB2K4
start: 857
end: 857
orientation: inline

lacOP
type: SequenceAnnotation
persistent identity: annotation1974331
display id: annotation1974331
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974331/range1974331/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974331/range1974331/1
type: Range
persistent identity: range1974331
display id: range1974331
version: 1
direction: direction/forward
top level: pSB2K4
start: 2064
end: 2238
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974327
display id: annotation1974327
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974327/range1974327/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974327/range1974327/1
type: Range
persistent identity: range1974327
display id: range1974327
version: 1
direction: direction/forward
top level: pSB2K4
start: 1581
end: 1591
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974324
display id: annotation1974324
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974324/range1974324/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974324/range1974324/1
type: Range
persistent identity: range1974324
display id: range1974324
version: 1
direction: direction/forward
top level: pSB2K4
start: 1497
end: 1515
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974294
display id: annotation1974294
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974294/range1974294/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974294/range1974294/1
type: Range
persistent identity: range1974294
display id: range1974294
version: 1
direction: direction/forward
top level: pSB2K4
start: 93
end: 93
orientation: inline

T->A
type: SequenceAnnotation
persistent identity: annotation1974298
display id: annotation1974298
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974298/range1974298/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974298/range1974298/1
type: Range
persistent identity: range1974298
display id: range1974298
version: 1
direction: direction/forward
top level: pSB2K4
start: 292
end: 292
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974311
display id: annotation1974311
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974311/range1974311/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974311/range1974311/1
type: Range
persistent identity: range1974311
display id: range1974311
version: 1
direction: direction/forward
top level: pSB2K4
start: 1091
end: 1091
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974299
display id: annotation1974299
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974299/range1974299/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974299/range1974299/1
type: Range
persistent identity: range1974299
display id: range1974299
version: 1
direction: direction/reverse
top level: pSB2K4
start: 394
end: 412
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974321
display id: annotation1974321
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974321/range1974321/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974321/range1974321/1
type: Range
persistent identity: range1974321
display id: range1974321
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1455
end: 1460
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974312
display id: annotation1974312
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974312/range1974312/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974312/range1974312/1
type: Range
persistent identity: range1974312
display id: range1974312
version: 1
direction: direction/forward
top level: pSB2K4
start: 1100
end: 1100
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation1974348
display id: annotation1974348
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974348/range1974348/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974348/range1974348/1
type: Range
persistent identity: range1974348
display id: range1974348
version: 1
direction: direction/forward
top level: pSB2K4
start: 3460
end: 4275
orientation: inline

pSB4A50
type: ComponentDefinition
persistent identity: BBa K1362095
display id: BBa_K1362095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4A50: Low copy BioBrick cloning/expression backbone carrying Amp resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0043
component: BBa_I50042
component: BBa_B0055
component: BBa_B0054
component: BBa_K1362463
component: BBa_G00102
component: BBa_B0042
component: BBa_G00000
component: BBa_G00001
component: BBa_G00100
component: BBa_G0003
component: BBa_G0004
component: BBa_P1002
component: BBa_B0053
component: BBa_B0044
component: BBa_B0062
component: BBa_G0002
sequence annotation: BBa_B0053
sequence annotation: BBa_K1362463
sequence annotation: BBa_B0055
sequence annotation: BBa_B0042
sequence annotation: BBa_G0003
sequence annotation: BBa_B0043
sequence annotation: BBa_G00100
sequence annotation: BBa_G0002
sequence annotation: BBa_G00102
sequence annotation: BBa_B0044
sequence annotation: BBa_G00001
sequence annotation: BBa_P1002
sequence annotation: BBa_I50042
sequence annotation: BBa_G0004
sequence annotation: BBa_B0054
sequence annotation: BBa_B0062
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_K1362095_sequence/1

BBa_B0043
type: Component
persistent identity: component2409838
display id: component2409838
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_I50042
type: Component
persistent identity: component2409812
display id: component2409812
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0055
type: Component
persistent identity: component2409826
display id: component2409826
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0054
type: Component
persistent identity: component2409779
display id: component2409779
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_K1362463
type: Component
persistent identity: component2409842
display id: component2409842
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_G00102
type: Component
persistent identity: component2409781
display id: component2409781
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component2409776
display id: component2409776
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00000
type: Component
persistent identity: component2409847
display id: component2409847
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00001
type: Component
persistent identity: component2409764
display id: component2409764
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00100
type: Component
persistent identity: component2409823
display id: component2409823
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0042
type: Component
persistent identity: component2409833
display id: component2409833
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0003
type: Component
persistent identity: component2409784
display id: component2409784
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G0004
type: Component
persistent identity: component2409819
display id: component2409819
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_P1002
type: Component
persistent identity: component2409818
display id: component2409818
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_B0053
type: Component
persistent identity: component2409821
display id: component2409821
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0044
type: Component
persistent identity: component2409769
display id: component2409769
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409783
display id: component2409783
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0002
type: Component
persistent identity: component2409813
display id: component2409813
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409821
display id: annotation2409821
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
type: Range
persistent identity: range2409821
display id: range2409821
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3179
end: 3250
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409842
display id: annotation2409842
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
type: Range
persistent identity: range2409842
display id: range2409842
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3374
end: 3424
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409826
display id: annotation2409826
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
type: Range
persistent identity: range2409826
display id: range2409826
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3271
end: 3348
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409776
display id: annotation2409776
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
type: Range
persistent identity: range2409776
display id: range2409776
version: 1
direction: direction/reverse
top level: pSB4A50
start: 35
end: 46
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409784
display id: annotation2409784
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
type: Range
persistent identity: range2409784
display id: range2409784
version: 1
direction: direction/reverse
top level: pSB4A50
start: 177
end: 183
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409838
display id: annotation2409838
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
type: Range
persistent identity: range2409838
display id: range2409838
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3361
end: 3373
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409823
display id: annotation2409823
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
type: Range
persistent identity: range2409823
display id: range2409823
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3251
end: 3270
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409813
display id: annotation2409813
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
type: Range
persistent identity: range2409813
display id: range2409813
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2221
end: 2228
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409781
display id: annotation2409781
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
type: Range
persistent identity: range2409781
display id: range2409781
version: 1
direction: direction/reverse
top level: pSB4A50
start: 116
end: 135
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409769
display id: annotation2409769
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
type: Range
persistent identity: range2409769
display id: range2409769
version: 1
direction: direction/reverse
top level: pSB4A50
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409833
display id: annotation2409833
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
type: Range
persistent identity: range2409833
display id: range2409833
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3349
end: 3360
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409764
display id: annotation2409764
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
type: Range
persistent identity: range2409764
display id: range2409764
version: 1
direction: direction/reverse
top level: pSB4A50
start: 1
end: 21
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2409818
display id: annotation2409818
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
component: BBa_P1002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
type: Range
persistent identity: range2409818
display id: range2409818
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2229
end: 3171
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409812
display id: annotation2409812
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
type: Range
persistent identity: range2409812
display id: range2409812
version: 1
direction: direction/reverse
top level: pSB4A50
start: 184
end: 2220
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409819
display id: annotation2409819
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
type: Range
persistent identity: range2409819
display id: range2409819
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3172
end: 3178
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409779
display id: annotation2409779
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
type: Range
persistent identity: range2409779
display id: range2409779
version: 1
direction: direction/reverse
top level: pSB4A50
start: 47
end: 115
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409783
display id: annotation2409783
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
type: Range
persistent identity: range2409783
display id: range2409783
version: 1
direction: direction/reverse
top level: pSB4A50
start: 136
end: 176
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409847
display id: annotation2409847
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
type: Range
persistent identity: range2409847
display id: range2409847
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3425
end: 3446
orientation: inline

BBa_K260007
type: ComponentDefinition
persistent identity: BBa K260007
display id: BBa_K260007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMA-@CC2FosMB-05000bp
creator: Kaj Bernhardt
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _356_
m user id: 0
owner id: 1072
owning group id: 57
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This plasmid backbone is based on pMA from Geneart. It has BioBrick prefix and suffix flanked on both sides by 50 bp of homology arms for insertion of the contained BioBrick into the 05000 locus of pCC2FosMB (BBa_K260001) by recombineering. The pMA-@CC2FosMB-05000bp backbone is available with BBa_K260015, which is composed of promoter-FRT-dhfr. If the PvuII fragment from BBa_K260007-BBa_K260015 is transferred by recombineering to the the 05000 locus of BBa_K260001-BBa_K260016, the intervening sequence between the FRT sites of BBa_K260015 and BBa_K260016 on the BBa_K260001 backbone will be 5,000 bp exactly. These plasmid backbones are similar and all allow recombineering-mediated transfer of the contained BioBrick (typically BBa_K260015) to the pCC2FosMB backbone BBa_K2600001. They differ in the homology arms, which determines the locus of insertion into pCC2FosMB. The bp numbers denote final inter-FRT distance as described above: BBa_K260004: pMA-CC2FosMB-00500bp BBa_K260005: pMA-CC2FosMB-01000bp BBa_K260006: pMA-CC2FosMB-02000bp BBa_K260007: pMA-CC2FosMB-05000bp BBa_K260008: pMA-RQ-CC2FosMB-10000bp
mutable notes: The 50 bp homologies were designed to delete 100 bp of BBa_K260001-BBa_K260016 and put the FRT site of BBa_K260015 (if used with the present backbone BBa_K260007) at a defined distance from the FRT site of BBa_K260016.
mutable provenance: Geneart plasmid pMA, with 2x 50 bp from pCC2FosMB K260001.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BBa_K260001 (pCC2FosMB) 5' homology FRT-05000bp
sequence annotation: PvuII site
sequence annotation: BBa_K260001 (pCC2FosMB) 3' homology FRT-05000bp
sequence annotation: prefix
sequence annotation: AmpR
sequence annotation: suffix
sequence annotation: ColE1 origin
sequence: https://synbiohub.org/public/igem/BBa_K260007_sequence/1

BBa_K260001 (pCC2FosMB) 5' homology FRT-05000bp
type: SequenceAnnotation
persistent identity: annotation2049443
display id: annotation2049443
version: 1
top level: BBa_K260007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260007/annotation2049443/range2049443/1

https://synbiohub.org/public/igem/BBa_K260007/annotation2049443/range2049443/1
type: Range
persistent identity: range2049443
display id: range2049443
version: 1
direction: direction/forward
top level: BBa_K260007
start: 2474
end: 2543
orientation: inline

PvuII site
type: SequenceAnnotation
persistent identity: annotation2049426
display id: annotation2049426
version: 1
top level: BBa_K260007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K260007/annotation2049426/range2049426/1

https://synbiohub.org/public/igem/BBa_K260007/annotation2049426/range2049426/1
type: Range
persistent identity: range2049426
display id: range2049426
version: 1
direction: direction/forward
top level: BBa_K260007
start: 92
end: 97
orientation: inline

BBa_K260001 (pCC2FosMB) 3' homology FRT-05000bp
type: SequenceAnnotation
persistent identity: annotation2049417
display id: annotation2049417
version: 1
top level: BBa_K260007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260007/annotation2049417/range2049417/1

https://synbiohub.org/public/igem/BBa_K260007/annotation2049417/range2049417/1
type: Range
persistent identity: range2049417
display id: range2049417
version: 1
direction: direction/forward
top level: BBa_K260007
start: 22
end: 91
orientation: inline

PvuII site
type: SequenceAnnotation
persistent identity: annotation2049428
display id: annotation2049428
version: 1
top level: BBa_K260007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K260007/annotation2049428/range2049428/1

https://synbiohub.org/public/igem/BBa_K260007/annotation2049428/range2049428/1
type: Range
persistent identity: range2049428
display id: range2049428
version: 1
direction: direction/forward
top level: BBa_K260007
start: 2468
end: 2473
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2049448
display id: annotation2049448
version: 1
top level: BBa_K260007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260007/annotation2049448/range2049448/1

https://synbiohub.org/public/igem/BBa_K260007/annotation2049448/range2049448/1
type: Range
persistent identity: range2049448
display id: range2049448
version: 1
direction: direction/forward
top level: BBa_K260007
start: 2544
end: 2565
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2049434
display id: annotation2049434
version: 1
top level: BBa_K260007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260007/annotation2049434/range2049434/1

https://synbiohub.org/public/igem/BBa_K260007/annotation2049434/range2049434/1
type: Range
persistent identity: range2049434
display id: range2049434
version: 1
direction: direction/forward
top level: BBa_K260007
start: 609
end: 1469
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2049413
display id: annotation2049413
version: 1
top level: BBa_K260007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260007/annotation2049413/range2049413/1

https://synbiohub.org/public/igem/BBa_K260007/annotation2049413/range2049413/1
type: Range
persistent identity: range2049413
display id: range2049413
version: 1
direction: direction/forward
top level: BBa_K260007
start: 1
end: 21
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation2049427
display id: annotation2049427
version: 1
top level: BBa_K260007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260007/annotation2049427/range2049427/1

https://synbiohub.org/public/igem/BBa_K260007/annotation2049427/range2049427/1
type: Range
persistent identity: range2049427
display id: range2049427
version: 1
direction: direction/forward
top level: BBa_K260007
start: 1617
end: 2284
orientation: inline

BBa_K268000
type: ComponentDefinition
persistent identity: BBa K268000
display id: BBa_K268000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB6A0
creator: Nora Yucel
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _379_
m user id: 0
owner id: 2483
owning group id: 170
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Biobricked plasmid backbone pRS314 with XbaI, PstI and SpeI sites removed from outside the MCS. One of a series of pBluescript based yeast centrometer vectors developed by Sikorski R.S., Hieter P (Genetics 122:19-27(1989)). Confers Trp resistence in yeast and ampicillan resistance in E. coli where it can be transformed and amplified for cloning purposes.
mutable notes: Removal of non-biobrick compatible restriction sites.
mutable provenance: Unmodified version can be found by accession number U03440.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: F1_Ori, M13_Ori
sequence annotation: Biobrick prefix
sequence annotation: M13F
sequence annotation: Biobrick suffix
sequence annotation: CEN6_ARS4
sequence annotation: AmpR
sequence annotation: ColE1_Origin
sequence annotation: TRP1
sequence: https://synbiohub.org/public/igem/BBa_K268000_sequence/1

F1_Ori, M13_Ori
type: SequenceAnnotation
persistent identity: annotation2062712
display id: annotation2062712
version: 1
top level: BBa_K268000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062712/range2062712/1

https://synbiohub.org/public/igem/BBa_K268000/annotation2062712/range2062712/1
type: Range
persistent identity: range2062712
display id: range2062712
version: 1
direction: direction/forward
top level: BBa_K268000
start: 264
end: 719
orientation: inline

Biobrick prefix
type: SequenceAnnotation
persistent identity: annotation2062717
display id: annotation2062717
version: 1
top level: BBa_K268000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062717/range2062717/1

https://synbiohub.org/public/igem/BBa_K268000/annotation2062717/range2062717/1
type: Range
persistent identity: range2062717
display id: range2062717
version: 1
direction: direction/forward
top level: BBa_K268000
start: 4793
end: 4814
orientation: inline

M13F
type: SequenceAnnotation
persistent identity: annotation2062713
display id: annotation2062713
version: 1
top level: BBa_K268000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062713/range2062713/1

https://synbiohub.org/public/igem/BBa_K268000/annotation2062713/range2062713/1
type: Range
persistent identity: range2062713
display id: range2062713
version: 1
direction: direction/forward
top level: BBa_K268000
start: 1837
end: 1854
orientation: inline

Biobrick suffix
type: SequenceAnnotation
persistent identity: annotation2062715
display id: annotation2062715
version: 1
top level: BBa_K268000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062715/range2062715/1

https://synbiohub.org/public/igem/BBa_K268000/annotation2062715/range2062715/1
type: Range
persistent identity: range2062715
display id: range2062715
version: 1
direction: direction/forward
top level: BBa_K268000
start: 1
end: 21
orientation: inline

CEN6_ARS4
type: SequenceAnnotation
persistent identity: annotation2062718
display id: annotation2062718
version: 1
top level: BBa_K268000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062718/range2062718/1

https://synbiohub.org/public/igem/BBa_K268000/annotation2062718/range2062718/1
type: Range
persistent identity: range2062718
display id: range2062718
version: 1
direction: direction/forward
top level: BBa_K268000
start: 2022
end: 2540
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2062714
display id: annotation2062714
version: 1
top level: BBa_K268000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062714/range2062714/1

https://synbiohub.org/public/igem/BBa_K268000/annotation2062714/range2062714/1
type: Range
persistent identity: range2062714
display id: range2062714
version: 1
direction: direction/forward
top level: BBa_K268000
start: 2870
end: 3529
orientation: inline

ColE1_Origin
type: SequenceAnnotation
persistent identity: annotation2062716
display id: annotation2062716
version: 1
top level: BBa_K268000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062716/range2062716/1

https://synbiohub.org/public/igem/BBa_K268000/annotation2062716/range2062716/1
type: Range
persistent identity: range2062716
display id: range2062716
version: 1
direction: direction/forward
top level: BBa_K268000
start: 3627
end: 4609
orientation: inline

TRP1
type: SequenceAnnotation
persistent identity: annotation2062711
display id: annotation2062711
version: 1
top level: BBa_K268000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K268000/annotation2062711/range2062711/1

https://synbiohub.org/public/igem/BBa_K268000/annotation2062711/range2062711/1
type: Range
persistent identity: range2062711
display id: range2062711
version: 1
direction: direction/reverse
top level: BBa_K268000
start: 817
end: 1490
orientation: inline

BBa_K1480012
type: ComponentDefinition
persistent identity: BBa K1480012
display id: BBa_K1480012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3 + BF and AF TALEN (K1480001) Target sites
creator: Roberto Alejandro Valderrama Alfaro
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1860_
m user id: 0
owner id: 19018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Backbone vector of pSB1C3 that contains two modifications with a TALEN target after and before the origin of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains two TALEN target sites (AF+BF)
mutable notes: Modifications where inserted by PCR on both directions due to TALEN specificity.
mutable provenance: Derived from pSB1C3 plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1480012_sequence/1

BBa_K390000
type: ComponentDefinition
persistent identity: BBa K390000
display id: BBa_K390000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A3_Int (BamHI, XhoI, NdeI, NheI added)
creator: Cody Tramp
created: 2010-07-31T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _518_
m user id: 0
owner id: 6788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is based on pSB1A3, with BamHI, XhoI, NdeI and NheI sites added through site-directed mutagenesis. This plasmid backbone retains compatibility with BioBricks (except with RFC 21 - BamBgl), its antibiotic resistance, and its high copy number in E. coli. The additional restriction sites allow the addition of DNA sequences homologous to genomic sequences in Synechocystis sp. PCC 6803 and other species, enabling the plasmid to be used to integrate the BioBrick construct and an antibiotic resistance gene into the chromosome at a specific location through homologous recombination. Integration sequences can be designed so that the BioBrick construct is added in the middle of a gene knocking the gene out in the process, or between two genes without interfering with native expression. The BioBrick construct can be assembled in E. coli cells without changes to the standard procedure, and when completely assemble, the plasmid can be introduced into the target species. Synechocystis sp. PCC 6803 naturally takes up DNA from its environment, and if sequences are homologous to regions of the genome, will naturally undergo homologous recombination. Other species may lack one or both of these abilities, and the addition of another plasmid carrying a recombinase gene may be necessary for genomic integration.
mutable notes: BamHI, XhoI, NdeI, and NheI sites were mutated in using Stratagene's site-directed mutagenesis kit, the sites were mutated in by changing the nucleotide sequence, without the addition or removal of nucleotides.
mutable provenance: pSB1A3 with BamHI, XhoI, NdeI, and NheI sites mutated in outside of the terminator regions.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: XhoI Site
sequence annotation: VR
sequence annotation: E. coli his operon terminator
sequence annotation: g > a
sequence annotation: VF2
sequence annotation: end of terminator
sequence annotation: AmpR (rev)
sequence annotation: terminator stem loop
sequence annotation: c > t
sequence annotation: Ori
sequence annotation: BamHI Site
sequence annotation: NheI Site
sequence annotation: rep(pMB1) (rev)
sequence annotation: a > g
sequence annotation: suffix
sequence annotation: deletion of two bases
sequence annotation: prefix
sequence annotation: NdeI site
sequence annotation: t > a
sequence: https://synbiohub.org/public/igem/BBa_K390000_sequence/1

XhoI Site
type: SequenceAnnotation
persistent identity: annotation2121040
display id: annotation2121040
version: 1
top level: BBa_K390000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121040/range2121040/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121040/range2121040/1
type: Range
persistent identity: range2121040
display id: range2121040
version: 1
direction: direction/forward
top level: BBa_K390000
start: 2009
end: 2014
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2121030
display id: annotation2121030
version: 1
top level: BBa_K390000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121030/range2121030/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121030/range2121030/1
type: Range
persistent identity: range2121030
display id: range2121030
version: 1
direction: direction/reverse
top level: BBa_K390000
start: 157
end: 176
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2121027
display id: annotation2121027
version: 1
top level: BBa_K390000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121027/range2121027/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121027/range2121027/1
type: Range
persistent identity: range2121027
display id: range2121027
version: 1
direction: direction/forward
top level: BBa_K390000
start: 22
end: 93
orientation: inline

g > a
type: SequenceAnnotation
persistent identity: annotation2121032
display id: annotation2121032
version: 1
top level: BBa_K390000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121032/range2121032/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121032/range2121032/1
type: Range
persistent identity: range2121032
display id: range2121032
version: 1
direction: direction/forward
top level: BBa_K390000
start: 219
end: 219
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2121043
display id: annotation2121043
version: 1
top level: BBa_K390000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121043/range2121043/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121043/range2121043/1
type: Range
persistent identity: range2121043
display id: range2121043
version: 1
direction: direction/forward
top level: BBa_K390000
start: 2017
end: 2037
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2121044
display id: annotation2121044
version: 1
top level: BBa_K390000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121044/range2121044/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121044/range2121044/1
type: Range
persistent identity: range2121044
display id: range2121044
version: 1
direction: direction/forward
top level: BBa_K390000
start: 2094
end: 2097
orientation: inline

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation2121037
display id: annotation2121037
version: 1
top level: BBa_K390000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121037/range2121037/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121037/range2121037/1
type: Range
persistent identity: range2121037
display id: range2121037
version: 1
direction: direction/reverse
top level: BBa_K390000
start: 1025
end: 1895
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2121045
display id: annotation2121045
version: 1
top level: BBa_K390000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121045/range2121045/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121045/range2121045/1
type: Range
persistent identity: range2121045
display id: range2121045
version: 1
direction: direction/forward
top level: BBa_K390000
start: 2107
end: 2126
orientation: inline

c > t
type: SequenceAnnotation
persistent identity: annotation2121034
display id: annotation2121034
version: 1
top level: BBa_K390000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121034/range2121034/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121034/range2121034/1
type: Range
persistent identity: range2121034
display id: range2121034
version: 1
direction: direction/forward
top level: BBa_K390000
start: 232
end: 232
orientation: inline

Ori
type: SequenceAnnotation
persistent identity: annotation2121036
display id: annotation2121036
version: 1
top level: BBa_K390000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121036/range2121036/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121036/range2121036/1
type: Range
persistent identity: range2121036
display id: range2121036
version: 1
direction: direction/reverse
top level: BBa_K390000
start: 276
end: 276
orientation: inline

BamHI Site
type: SequenceAnnotation
persistent identity: annotation2121038
display id: annotation2121038
version: 1
top level: BBa_K390000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121038/range2121038/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121038/range2121038/1
type: Range
persistent identity: range2121038
display id: range2121038
version: 1
direction: direction/forward
top level: BBa_K390000
start: 1992
end: 1997
orientation: inline

NheI Site
type: SequenceAnnotation
persistent identity: annotation2121033
display id: annotation2121033
version: 1
top level: BBa_K390000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121033/range2121033/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121033/range2121033/1
type: Range
persistent identity: range2121033
display id: range2121033
version: 1
direction: direction/forward
top level: BBa_K390000
start: 230
end: 235
orientation: inline

c > t
type: SequenceAnnotation
persistent identity: annotation2121041
display id: annotation2121041
version: 1
top level: BBa_K390000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121041/range2121041/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121041/range2121041/1
type: Range
persistent identity: range2121041
display id: range2121041
version: 1
direction: direction/forward
top level: BBa_K390000
start: 2010
end: 2010
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2121035
display id: annotation2121035
version: 1
top level: BBa_K390000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121035/range2121035/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121035/range2121035/1
type: Range
persistent identity: range2121035
display id: range2121035
version: 1
direction: direction/reverse
top level: BBa_K390000
start: 261
end: 875
orientation: inline

a > g
type: SequenceAnnotation
persistent identity: annotation2121042
display id: annotation2121042
version: 1
top level: BBa_K390000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121042/range2121042/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121042/range2121042/1
type: Range
persistent identity: range2121042
display id: range2121042
version: 1
direction: direction/forward
top level: BBa_K390000
start: 2014
end: 2014
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2121026
display id: annotation2121026
version: 1
top level: BBa_K390000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121026/range2121026/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121026/range2121026/1
type: Range
persistent identity: range2121026
display id: range2121026
version: 1
direction: direction/forward
top level: BBa_K390000
start: 1
end: 21
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation2121029
display id: annotation2121029
version: 1
top level: BBa_K390000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121029/range2121029/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121029/range2121029/1
type: Range
persistent identity: range2121029
display id: range2121029
version: 1
direction: direction/forward
top level: BBa_K390000
start: 34
end: 35
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2121028
display id: annotation2121028
version: 1
top level: BBa_K390000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121028/range2121028/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121028/range2121028/1
type: Range
persistent identity: range2121028
display id: range2121028
version: 1
direction: direction/forward
top level: BBa_K390000
start: 30
end: 64
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2121046
display id: annotation2121046
version: 1
top level: BBa_K390000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121046/range2121046/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121046/range2121046/1
type: Range
persistent identity: range2121046
display id: range2121046
version: 1
direction: direction/forward
top level: BBa_K390000
start: 2136
end: 2157
orientation: inline

NdeI site
type: SequenceAnnotation
persistent identity: annotation2121031
display id: annotation2121031
version: 1
top level: BBa_K390000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121031/range2121031/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121031/range2121031/1
type: Range
persistent identity: range2121031
display id: range2121031
version: 1
direction: direction/forward
top level: BBa_K390000
start: 216
end: 221
orientation: inline

t > a
type: SequenceAnnotation
persistent identity: annotation2121039
display id: annotation2121039
version: 1
top level: BBa_K390000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K390000/annotation2121039/range2121039/1

https://synbiohub.org/public/igem/BBa_K390000/annotation2121039/range2121039/1
type: Range
persistent identity: range2121039
display id: range2121039
version: 1
direction: direction/forward
top level: BBa_K390000
start: 1995
end: 1995
orientation: inline

BBa_K799025
type: ComponentDefinition
persistent identity: BBa K799025
display id: BBa_K799025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3 yGG ORF acceptor vector
creator: Daniel Wolozny
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1055_
m user id: 0
owner id: 8447
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The original psb1c3 backbone was modified in order to allow for Golden Gate enabled parts to easily become biobricks.
mutable notes: Golden Gate Compatible.
mutable provenance: Igem Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Psb1C3 Modified Backbone
sequence: https://synbiohub.org/public/igem/BBa_K799025_sequence/1

Psb1C3 Modified Backbone
type: SequenceAnnotation
persistent identity: annotation2197874
display id: annotation2197874
version: 1
top level: BBa_K799025
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K799025/annotation2197874/range2197874/1

https://synbiohub.org/public/igem/BBa_K799025/annotation2197874/range2197874/1
type: Range
persistent identity: range2197874
display id: range2197874
version: 1
direction: direction/forward
top level: BBa_K799025
start: 1
end: 2027
orientation: inline

BBa_J99003
type: ComponentDefinition
persistent identity: BBa J99003
display id: BBa_J99003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSUlib ori_p15A; [KmR]
creator: David Bikard
created: 2009-12-17T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _412_
m user id: 0
owner id: 1481
owning group id: 412
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid with a P15A origin of replication
mutable notes: Modification of the MCS of pSU38delta by inverse PCR with oligo 5'-GCTTCTAGAGTACTAGTAGCGGCCGCTGCAGATTCCGAAGCCCAACCTTTCATA-3' and 5'-AGTACTCTAGAAGCGGCCGCGAATTCTAACATACGAGCCGGAAGCAT-3'
mutable provenance: pSU38delta (Biskri et al., 2005)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: aph [KmR]
sequence annotation: P15A rep
sequence: https://synbiohub.org/public/igem/BBa_J99003_sequence/1

aph [KmR]
type: SequenceAnnotation
persistent identity: annotation2065229
display id: annotation2065229
version: 1
top level: BBa_J99003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J99003/annotation2065229/range2065229/1

https://synbiohub.org/public/igem/BBa_J99003/annotation2065229/range2065229/1
type: Range
persistent identity: range2065229
display id: range2065229
version: 1
direction: direction/reverse
top level: BBa_J99003
start: 125
end: 919
orientation: inline

P15A rep
type: SequenceAnnotation
persistent identity: annotation2065228
display id: annotation2065228
version: 1
top level: BBa_J99003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J99003/annotation2065228/range2065228/1

https://synbiohub.org/public/igem/BBa_J99003/annotation2065228/range2065228/1
type: Range
persistent identity: range2065228
display id: range2065228
version: 1
direction: direction/forward
top level: BBa_J99003
start: 1281
end: 2069
orientation: inline

BBa_K592201
type: ComponentDefinition
persistent identity: BBa K592201
display id: BBa_K592201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy Lambda Red recombineering compatible plasmid
creator: Antonio Ascue Avalos
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 10133
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K592201 is a BioBrick friendly vector with low to medium copy p15A replication origin (BBa_I50032) and chloramphenicol antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22, and the chloramphenicol resistance marker is flanked by FRT sites so that the resistance marker can be removed after chromosomal integration using the FLP recombinase.
mutable notes: To make the plasmid usable for Lambda Red recombineering, all sequences with homologies to the E coli chromosome had to be removed. To accomplish this, the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. To be able to remove the antibiotic resistance marker after chromosomal integration, the resistance marker from the pKD3 plasmid (Datsenko and Wanner, 2000) was used. The resistance marker can be removed after chromosomal integration using the FLP recombinase, leaving only the plasmid insert and a scar behind.
mutable provenance: The P22 late terminator come from the genome of the Salmonella phage P22 (GenBank: AF217253.1, 19662..19702), and the chloramphenicol resistance marker flanked by FRT sites comes from the pKD3 plasmid (Datsenko and Wanner, 2000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Chloramphenicol resistant marker
sequence annotation: Terminator BBa_B0054
sequence annotation: T3 terminator BBa_B0055
sequence annotation: Terminator BBa_B0062
sequence annotation: FRT site
sequence annotation: translational stop BBa_B0042
sequence annotation: p15A ori BBa_I50032
sequence annotation: Translational stop BBa_B0042
sequence annotation: P22 late terminator
sequence annotation: VF2 site BBa_G00100
sequence annotation: VR site BBa_G00102
sequence annotation: Lambda Red R primer
sequence annotation: L-red_F_primer
sequence: https://synbiohub.org/public/igem/BBa_K592201_sequence/1

Chloramphenicol resistant marker
type: SequenceAnnotation
persistent identity: annotation2137508
display id: annotation2137508
version: 1
top level: BBa_K592201
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137508/range2137508/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137508/range2137508/1
type: Range
persistent identity: range2137508
display id: range2137508
version: 1
direction: direction/forward
top level: BBa_K592201
start: 2035
end: 2673
orientation: inline

Terminator BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2137468
display id: annotation2137468
version: 1
top level: BBa_K592201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137468/range2137468/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137468/range2137468/1
type: Range
persistent identity: range2137468
display id: range2137468
version: 1
direction: direction/forward
top level: BBa_K592201
start: 47
end: 115
orientation: inline

T3 terminator BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2137574
display id: annotation2137574
version: 1
top level: BBa_K592201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137574/range2137574/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137574/range2137574/1
type: Range
persistent identity: range2137574
display id: range2137574
version: 1
direction: direction/forward
top level: BBa_K592201
start: 2843
end: 2920
orientation: inline

Terminator BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2137500
display id: annotation2137500
version: 1
top level: BBa_K592201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137500/range2137500/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137500/range2137500/1
type: Range
persistent identity: range2137500
display id: range2137500
version: 1
direction: direction/forward
top level: BBa_K592201
start: 136
end: 176
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2137507
display id: annotation2137507
version: 1
top level: BBa_K592201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137507/range2137507/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137507/range2137507/1
type: Range
persistent identity: range2137507
display id: range2137507
version: 1
direction: direction/forward
top level: BBa_K592201
start: 1785
end: 1826
orientation: inline

translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2137622
display id: annotation2137622
version: 1
top level: BBa_K592201
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137622/range2137622/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137622/range2137622/1
type: Range
persistent identity: range2137622
display id: range2137622
version: 1
direction: direction/forward
top level: BBa_K592201
start: 2921
end: 2932
orientation: inline

p15A ori BBa_I50032
type: SequenceAnnotation
persistent identity: annotation2137501
display id: annotation2137501
version: 1
top level: BBa_K592201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137501/range2137501/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137501/range2137501/1
type: Range
persistent identity: range2137501
display id: range2137501
version: 1
direction: direction/forward
top level: BBa_K592201
start: 184
end: 1748
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2137467
display id: annotation2137467
version: 1
top level: BBa_K592201
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137467/range2137467/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137467/range2137467/1
type: Range
persistent identity: range2137467
display id: range2137467
version: 1
direction: direction/forward
top level: BBa_K592201
start: 35
end: 46
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2137512
display id: annotation2137512
version: 1
top level: BBa_K592201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137512/range2137512/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137512/range2137512/1
type: Range
persistent identity: range2137512
display id: range2137512
version: 1
direction: direction/forward
top level: BBa_K592201
start: 2782
end: 2822
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2137509
display id: annotation2137509
version: 1
top level: BBa_K592201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137509/range2137509/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137509/range2137509/1
type: Range
persistent identity: range2137509
display id: range2137509
version: 1
direction: direction/forward
top level: BBa_K592201
start: 2723
end: 2756
orientation: inline

VF2 site BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2137548
display id: annotation2137548
version: 1
top level: BBa_K592201
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137548/range2137548/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137548/range2137548/1
type: Range
persistent identity: range2137548
display id: range2137548
version: 1
direction: direction/forward
top level: BBa_K592201
start: 2823
end: 2842
orientation: inline

VR site BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2137469
display id: annotation2137469
version: 1
top level: BBa_K592201
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137469/range2137469/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137469/range2137469/1
type: Range
persistent identity: range2137469
display id: range2137469
version: 1
direction: direction/reverse
top level: BBa_K592201
start: 116
end: 135
orientation: inline

Lambda Red R primer
type: SequenceAnnotation
persistent identity: annotation2136905
display id: annotation2136905
version: 1
top level: BBa_K592201
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2136905/range2136905/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2136905/range2136905/1
type: Range
persistent identity: range2136905
display id: range2136905
version: 1
direction: direction/reverse
top level: BBa_K592201
start: 20
end: 41
orientation: inline

L-red_F_primer
type: SequenceAnnotation
persistent identity: annotation2137502
display id: annotation2137502
version: 1
top level: BBa_K592201
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592201/annotation2137502/range2137502/1

https://synbiohub.org/public/igem/BBa_K592201/annotation2137502/range2137502/1
type: Range
persistent identity: range2137502
display id: range2137502
version: 1
direction: direction/forward
top level: BBa_K592201
start: 1726
end: 1745
orientation: inline

BBa_K563055
type: ComponentDefinition
persistent identity: BBa K563055
display id: BBa_K563055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A11, reconstructed from pSB1A2 with NcoI, XhoI, BamHI and PstI
creator: Guo Jing
created: 2011-10-04T11:00:00Z
modified: 2015-05-08T01:12:42Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _731_
m user id: 0
owner id: 10236
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1A2 is a existing BioBrick, and we reconstruct it to make our connection easier. We add NcoI, XhoI, BamHI, and PstI into the vector.
mutable notes: In order to make our mutation work easier, we separate Tor2 into three parts. so we add fout certain restriction sites into the vector to make the connection easier to conduct.
mutable provenance: pSB1A2 is from BioBrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: NcoI
sequence annotation: PstI
sequence annotation: XhoI
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_K563055_sequence/1

NcoI
type: SequenceAnnotation
persistent identity: annotation2154493
display id: annotation2154493
version: 1
top level: BBa_K563055
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K563055/annotation2154493/range2154493/1

https://synbiohub.org/public/igem/BBa_K563055/annotation2154493/range2154493/1
type: Range
persistent identity: range2154493
display id: range2154493
version: 1
direction: direction/forward
top level: BBa_K563055
start: 121
end: 126
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2154498
display id: annotation2154498
version: 1
top level: BBa_K563055
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K563055/annotation2154498/range2154498/1

https://synbiohub.org/public/igem/BBa_K563055/annotation2154498/range2154498/1
type: Range
persistent identity: range2154498
display id: range2154498
version: 1
direction: direction/forward
top level: BBa_K563055
start: 167
end: 172
orientation: inline

XhoI
type: SequenceAnnotation
persistent identity: annotation2154494
display id: annotation2154494
version: 1
top level: BBa_K563055
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K563055/annotation2154494/range2154494/1

https://synbiohub.org/public/igem/BBa_K563055/annotation2154494/range2154494/1
type: Range
persistent identity: range2154494
display id: range2154494
version: 1
direction: direction/forward
top level: BBa_K563055
start: 133
end: 138
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2154496
display id: annotation2154496
version: 1
top level: BBa_K563055
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K563055/annotation2154496/range2154496/1

https://synbiohub.org/public/igem/BBa_K563055/annotation2154496/range2154496/1
type: Range
persistent identity: range2154496
display id: range2154496
version: 1
direction: direction/forward
top level: BBa_K563055
start: 153
end: 158
orientation: inline

pSBBs4S
type: ComponentDefinition
persistent identity: BBa K823024
display id: BBa_K823024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs4S-Pxyl: Integrative expression vector for Bacillus subtilis</i
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
mutable provenance: This part is an igem compatible version of the Bacillus expression vector pXT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: thrB
sequence annotation: bla
sequence annotation: spc
sequence annotation: Pxly region
sequence annotation: thrC'
sequence annotation: hom
sequence annotation: 'thrC
sequence: https://synbiohub.org/public/igem/BBa_K823024_sequence/1

thrB
type: SequenceAnnotation
persistent identity: annotation2180242
display id: annotation2180242
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180242/range2180242/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180242/range2180242/1
type: Range
persistent identity: range2180242
display id: range2180242
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1453
end: 1863
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180243
display id: annotation2180243
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180243/range2180243/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180243/range2180243/1
type: Range
persistent identity: range2180243
display id: range2180243
version: 1
direction: direction/reverse
top level: pSBBs4S
start: 2716
end: 3576
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2180240
display id: annotation2180240
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180240/range2180240/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180240/range2180240/1
type: Range
persistent identity: range2180240
display id: range2180240
version: 1
direction: direction/forward
top level: pSBBs4S
start: 211
end: 885
orientation: inline

Pxly region
type: SequenceAnnotation
persistent identity: annotation2180246
display id: annotation2180246
version: 1
top level: pSBBs4S
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180246/range2180246/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180246/range2180246/1
type: Range
persistent identity: range2180246
display id: range2180246
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4539
end: 4763
orientation: inline

thrC'
type: SequenceAnnotation
persistent identity: annotation2180245
display id: annotation2180245
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180245/range2180245/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180245/range2180245/1
type: Range
persistent identity: range2180245
display id: range2180245
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4033
end: 4572
orientation: inline

hom
type: SequenceAnnotation
persistent identity: annotation2180244
display id: annotation2180244
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180244/range2180244/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180244/range2180244/1
type: Range
persistent identity: range2180244
display id: range2180244
version: 1
direction: direction/forward
top level: pSBBs4S
start: 3731
end: 4033
orientation: inline

'thrC
type: SequenceAnnotation
persistent identity: annotation2180241
display id: annotation2180241
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180241/range2180241/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180241/range2180241/1
type: Range
persistent identity: range2180241
display id: range2180241
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1028
end: 1456
orientation: inline

BBa_K359201
type: ComponentDefinition
persistent identity: BBa K359201
display id: BBa_K359201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB3K3-S-rbsRFP-P
creator: George Zarubin
created: 2010-07-27T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: true
group u list: _470_
m user id: 0
owner id: 7330
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Inspired by the measkit BBa_I20260 . The RFP measkit template with swappable promoter part, taken from BBa_J61002 .
mutable notes: Non-standard suffix and prefix of BBa_J6100 is inherited.
mutable provenance: BBa_J61002-BBa_J23101, pSB3K3-BBa_I20260
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: E1010
sequence annotation: KanR
sequence annotation: B0015
sequence annotation: SpeI
sequence annotation: PstI
sequence annotation: B0034
sequence annotation: p15A ori
sequence annotation: BB pre
sequence: https://synbiohub.org/public/igem/BBa_K359201_sequence/1

E1010
type: SequenceAnnotation
persistent identity: annotation2076040
display id: annotation2076040
version: 1
top level: BBa_K359201
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K359201/annotation2076040/range2076040/1

https://synbiohub.org/public/igem/BBa_K359201/annotation2076040/range2076040/1
type: Range
persistent identity: range2076040
display id: range2076040
version: 1
direction: direction/forward
top level: BBa_K359201
start: 27
end: 707
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2076042
display id: annotation2076042
version: 1
top level: BBa_K359201
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K359201/annotation2076042/range2076042/1

https://synbiohub.org/public/igem/BBa_K359201/annotation2076042/range2076042/1
type: Range
persistent identity: range2076042
display id: range2076042
version: 1
direction: direction/reverse
top level: BBa_K359201
start: 1108
end: 1923
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2076041
display id: annotation2076041
version: 1
top level: BBa_K359201
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K359201/annotation2076041/range2076041/1

https://synbiohub.org/public/igem/BBa_K359201/annotation2076041/range2076041/1
type: Range
persistent identity: range2076041
display id: range2076041
version: 1
direction: direction/forward
top level: BBa_K359201
start: 741
end: 869
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2076045
display id: annotation2076045
version: 1
top level: BBa_K359201
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K359201/annotation2076045/range2076045/1

https://synbiohub.org/public/igem/BBa_K359201/annotation2076045/range2076045/1
type: Range
persistent identity: range2076045
display id: range2076045
version: 1
direction: direction/forward
top level: BBa_K359201
start: 2
end: 7
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2076046
display id: annotation2076046
version: 1
top level: BBa_K359201
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K359201/annotation2076046/range2076046/1

https://synbiohub.org/public/igem/BBa_K359201/annotation2076046/range2076046/1
type: Range
persistent identity: range2076046
display id: range2076046
version: 1
direction: direction/forward
top level: BBa_K359201
start: 885
end: 890
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2076039
display id: annotation2076039
version: 1
top level: BBa_K359201
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K359201/annotation2076039/range2076039/1

https://synbiohub.org/public/igem/BBa_K359201/annotation2076039/range2076039/1
type: Range
persistent identity: range2076039
display id: range2076039
version: 1
direction: direction/forward
top level: BBa_K359201
start: 9
end: 20
orientation: inline

p15A ori
type: SequenceAnnotation
persistent identity: annotation2076043
display id: annotation2076043
version: 1
top level: BBa_K359201
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K359201/annotation2076043/range2076043/1

https://synbiohub.org/public/igem/BBa_K359201/annotation2076043/range2076043/1
type: Range
persistent identity: range2076043
display id: range2076043
version: 1
direction: direction/reverse
top level: BBa_K359201
start: 2255
end: 3079
orientation: inline

BB pre
type: SequenceAnnotation
persistent identity: annotation2076044
display id: annotation2076044
version: 1
top level: BBa_K359201
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K359201/annotation2076044/range2076044/1

https://synbiohub.org/public/igem/BBa_K359201/annotation2076044/range2076044/1
type: Range
persistent identity: range2076044
display id: range2076044
version: 1
direction: direction/forward
top level: BBa_K359201
start: 3598
end: 3619
orientation: inline

BBa_K1480011
type: ComponentDefinition
persistent identity: BBa K1480011
display id: BBa_K1480011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3 + BF TALEN (K1480001) Target site
creator: Roberto Alejandro Valderrama Alfaro
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1860_
m user id: 0
owner id: 19018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Backbone vector of pSB1C3 that contains a modification with a TALEN target site before the origin of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains a single TALEN target site (BF).
mutable notes: Modifications where inserted by PCR on both directions due to TALEN specificity.
mutable provenance: Derived from pSB1C3 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1480011_sequence/1

BBa_K1391017
type: ComponentDefinition
persistent identity: BBa K1391017
display id: BBa_K1391017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MAV1212-RFP
creator: Jeremy Gam
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1768_
m user id: 0
owner id: 22845
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RFP inside a MAV1212 backbone.
mutable notes: none
mutable provenance: provided by the Weiss lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1391017_sequence/1

BBa_K238026
type: ComponentDefinition
persistent identity: BBa K238026
display id: BBa_K238026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMA plasmid
creator: Annelien Verfaillie
created: 2009-10-03T11:00:00Z
modified: 2015-05-08T01:11:36Z
discontinued: false
dominant: false
group u list: _332_
m user id: 0
owner id: 4771
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part BBa_K238024, the lock, was sent to us by Geneart in this plasmid. It differs from pMA-RQ in that it has an extra suffix and 4 extra basepares after the second suffix, being TACT, making it 25bp longer.
mutable notes: none
mutable provenance: geneart
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: AmpR
sequence annotation: ColE1 origin
sequence annotation: suffix 1
sequence annotation: prefix
sequence annotation: suffix 2
sequence: https://synbiohub.org/public/igem/BBa_K238026_sequence/1

AmpR
type: SequenceAnnotation
persistent identity: annotation2028067
display id: annotation2028067
version: 1
top level: BBa_K238026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K238026/annotation2028067/range2028067/1

https://synbiohub.org/public/igem/BBa_K238026/annotation2028067/range2028067/1
type: Range
persistent identity: range2028067
display id: range2028067
version: 1
direction: direction/forward
top level: BBa_K238026
start: 488
end: 516
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation2028069
display id: annotation2028069
version: 1
top level: BBa_K238026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K238026/annotation2028069/range2028069/1

https://synbiohub.org/public/igem/BBa_K238026/annotation2028069/range2028069/1
type: Range
persistent identity: range2028069
display id: range2028069
version: 1
direction: direction/forward
top level: BBa_K238026
start: 1513
end: 2195
orientation: inline

suffix 1
type: SequenceAnnotation
persistent identity: annotation2028064
display id: annotation2028064
version: 1
top level: BBa_K238026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K238026/annotation2028064/range2028064/1

https://synbiohub.org/public/igem/BBa_K238026/annotation2028064/range2028064/1
type: Range
persistent identity: range2028064
display id: range2028064
version: 1
direction: direction/forward
top level: BBa_K238026
start: 1
end: 21
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2028066
display id: annotation2028066
version: 1
top level: BBa_K238026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K238026/annotation2028066/range2028066/1

https://synbiohub.org/public/igem/BBa_K238026/annotation2028066/range2028066/1
type: Range
persistent identity: range2028066
display id: range2028066
version: 1
direction: direction/forward
top level: BBa_K238026
start: 2417
end: 2438
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2028068
display id: annotation2028068
version: 1
top level: BBa_K238026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K238026/annotation2028068/range2028068/1

https://synbiohub.org/public/igem/BBa_K238026/annotation2028068/range2028068/1
type: Range
persistent identity: range2028068
display id: range2028068
version: 1
direction: direction/forward
top level: BBa_K238026
start: 558
end: 1415
orientation: inline

suffix 2
type: SequenceAnnotation
persistent identity: annotation2028065
display id: annotation2028065
version: 1
top level: BBa_K238026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K238026/annotation2028065/range2028065/1

https://synbiohub.org/public/igem/BBa_K238026/annotation2028065/range2028065/1
type: Range
persistent identity: range2028065
display id: range2028065
version: 1
direction: direction/forward
top level: BBa_K238026
start: 22
end: 42
orientation: inline

BBa_K1480019
type: ComponentDefinition
persistent identity: BBa K1480019
display id: BBa_K1480019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB3C5 + MID + BF + AF TALEN (K1480001) Target sites
creator: Roberto Alejandro Valderrama Alfaro
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1860_
m user id: 0
owner id: 19018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Backbone vector of pSB3C5 that contains three modifications with a TALEN target site on the center, after and before the origin of the plasmid. It can be used as a target for the respective TALEN enzyme in DNA edition projects or processing information systems. It contains three TALEN target sites (MID+BF+AF)
mutable notes: Modifications where inserted by PCR on both directions due to TALEN specificity.
mutable provenance: Derived from pSB3C5 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1480019_sequence/1

BBa_K533012
type: ComponentDefinition
persistent identity: BBa K533012
display id: BBa_K533012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: safety version of pSB1C3 plasmid
creator: ZHANG Yunxiao
created: 2011-10-22T11:00:00Z
modified: 2015-06-15T01:18:23Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _699_
m user id: 4206
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Deleted
bookmark: false
mutable description: The plasmid has a suicide gene and a mutation-controller sequence. It can hence eliminate devious mutations and help to restrain biobricks to only our engineered organisms.
mutable notes: Do not use commonly used commercial competent cells when using this plasmid. Special strains are required.
mutable provenance: derived from pSB1C3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VF2
sequence annotation: mutated EcoRI
sequence annotation: -10
sequence annotation: -35
sequence annotation: E. coli his operon terminator
sequence annotation: mutated I-SceI site
sequence annotation: Chloramphenicol resistance
sequence annotation: new EcoRI site
sequence annotation: ccdB
sequence annotation: VR
sequence annotation: pMB1
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K533012_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation2161083
display id: annotation2161083
version: 1
top level: BBa_K533012
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161083/range2161083/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161083/range2161083/1
type: Range
persistent identity: range2161083
display id: range2161083
version: 1
direction: direction/forward
top level: BBa_K533012
start: 1933
end: 1952
orientation: inline

mutated EcoRI
type: SequenceAnnotation
persistent identity: annotation2161042
display id: annotation2161042
version: 1
top level: BBa_K533012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161042/range2161042/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161042/range2161042/1
type: Range
persistent identity: range2161042
display id: range2161042
version: 1
direction: direction/forward
top level: BBa_K533012
start: 2049
end: 2054
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2161043
display id: annotation2161043
version: 1
top level: BBa_K533012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161043/range2161043/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161043/range2161043/1
type: Range
persistent identity: range2161043
display id: range2161043
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 2527
end: 2532
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2161044
display id: annotation2161044
version: 1
top level: BBa_K533012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161044/range2161044/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161044/range2161044/1
type: Range
persistent identity: range2161044
display id: range2161044
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 2558
end: 2563
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2161048
display id: annotation2161048
version: 1
top level: BBa_K533012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161048/range2161048/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161048/range2161048/1
type: Range
persistent identity: range2161048
display id: range2161048
version: 1
direction: direction/forward
top level: BBa_K533012
start: 22
end: 93
orientation: inline

mutated I-SceI site
type: SequenceAnnotation
persistent identity: annotation2161049
display id: annotation2161049
version: 1
top level: BBa_K533012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161049/range2161049/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161049/range2161049/1
type: Range
persistent identity: range2161049
display id: range2161049
version: 1
direction: direction/forward
top level: BBa_K533012
start: 2571
end: 2588
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2161047
display id: annotation2161047
version: 1
top level: BBa_K533012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161047/range2161047/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161047/range2161047/1
type: Range
persistent identity: range2161047
display id: range2161047
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 1109
end: 1819
orientation: inline

new EcoRI site
type: SequenceAnnotation
persistent identity: annotation2161041
display id: annotation2161041
version: 1
top level: BBa_K533012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161041/range2161041/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161041/range2161041/1
type: Range
persistent identity: range2161041
display id: range2161041
version: 1
direction: direction/forward
top level: BBa_K533012
start: 2590
end: 2595
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation2161040
display id: annotation2161040
version: 1
top level: BBa_K533012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161040/range2161040/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161040/range2161040/1
type: Range
persistent identity: range2161040
display id: range2161040
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 2061
end: 2366
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2161082
display id: annotation2161082
version: 1
top level: BBa_K533012
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161082/range2161082/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161082/range2161082/1
type: Range
persistent identity: range2161082
display id: range2161082
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 157
end: 176
orientation: inline

pMB1
type: SequenceAnnotation
persistent identity: annotation2161046
display id: annotation2161046
version: 1
top level: BBa_K533012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161046/range2161046/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161046/range2161046/1
type: Range
persistent identity: range2161046
display id: range2161046
version: 1
direction: direction/forward
top level: BBa_K533012
start: 261
end: 874
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2161045
display id: annotation2161045
version: 1
top level: BBa_K533012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161045/range2161045/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161045/range2161045/1
type: Range
persistent identity: range2161045
display id: range2161045
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 2022
end: 2041
orientation: inline

BBa_K1256001
type: ComponentDefinition
persistent identity: BBa K1256001
display id: BBa_K1256001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3-Plac-NB
creator: Pei-Hong Chen
created: 2014-05-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1614_
m user id: 0
owner id: 8995
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: TEST
mutable notes: TEST
mutable provenance: TEST
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Chloramphenicol resistance cassette
sequence annotation: ori
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K1256001_sequence/1

Chloramphenicol resistance cassette
type: SequenceAnnotation
persistent identity: annotation2378956
display id: annotation2378956
version: 1
top level: BBa_K1256001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1256001/annotation2378956/range2378956/1

https://synbiohub.org/public/igem/BBa_K1256001/annotation2378956/range2378956/1
type: Range
persistent identity: range2378956
display id: range2378956
version: 1
direction: direction/reverse
top level: BBa_K1256001
start: 2235
end: 2894
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2378955
display id: annotation2378955
version: 1
top level: BBa_K1256001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1256001/annotation2378955/range2378955/1

https://synbiohub.org/public/igem/BBa_K1256001/annotation2378955/range2378955/1
type: Range
persistent identity: range2378955
display id: range2378955
version: 1
direction: direction/forward
top level: BBa_K1256001
start: 1335
end: 1948
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2378952
display id: annotation2378952
version: 1
top level: BBa_K1256001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1256001/annotation2378952/range2378952/1

https://synbiohub.org/public/igem/BBa_K1256001/annotation2378952/range2378952/1
type: Range
persistent identity: range2378952
display id: range2378952
version: 1
direction: direction/forward
top level: BBa_K1256001
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2378951
display id: annotation2378951
version: 1
top level: BBa_K1256001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1256001/annotation2378951/range2378951/1

https://synbiohub.org/public/igem/BBa_K1256001/annotation2378951/range2378951/1
type: Range
persistent identity: range2378951
display id: range2378951
version: 1
direction: direction/forward
top level: BBa_K1256001
start: 1
end: 200
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2378954
display id: annotation2378954
version: 1
top level: BBa_K1256001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1256001/annotation2378954/range2378954/1

https://synbiohub.org/public/igem/BBa_K1256001/annotation2378954/range2378954/1
type: Range
persistent identity: range2378954
display id: range2378954
version: 1
direction: direction/forward
top level: BBa_K1256001
start: 947
end: 1075
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2378953
display id: annotation2378953
version: 1
top level: BBa_K1256001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1256001/annotation2378953/range2378953/1

https://synbiohub.org/public/igem/BBa_K1256001/annotation2378953/range2378953/1
type: Range
persistent identity: range2378953
display id: range2378953
version: 1
direction: direction/forward
top level: BBa_K1256001
start: 227
end: 932
orientation: inline

pSB8C15
type: ComponentDefinition
persistent identity: BBa K864017
display id: BBa_K864017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8C15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and Chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Lux terminator
sequence annotation: cmR
sequence annotation: tonB terminator
sequence annotation: T3 terminator
sequence annotation: Promoter
sequence annotation: -35
sequence annotation: VF2 forward primer binding site
sequence annotation: VR reverse primer binding site
sequence annotation: P22 late terminator
sequence annotation: pSC101-ts ori
sequence annotation: rrnC
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K864017_sequence/1

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2203159
display id: annotation2203159
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203159/range2203159/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203159/range2203159/1
type: Range
persistent identity: range2203159
display id: range2203159
version: 1
direction: direction/reverse
top level: pSB8C15
start: 2454
end: 2499
orientation: inline

cmR
type: SequenceAnnotation
persistent identity: annotation2203155
display id: annotation2203155
version: 1
top level: pSB8C15
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203155/range2203155/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203155/range2203155/1
type: Range
persistent identity: range2203155
display id: range2203155
version: 1
direction: direction/reverse
top level: pSB8C15
start: 2506
end: 3168
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2203157
display id: annotation2203157
version: 1
top level: pSB8C15
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203157/range2203157/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203157/range2203157/1
type: Range
persistent identity: range2203157
display id: range2203157
version: 1
direction: direction/forward
top level: pSB8C15
start: 47
end: 115
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2203161
display id: annotation2203161
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203161/range2203161/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203161/range2203161/1
type: Range
persistent identity: range2203161
display id: range2203161
version: 1
direction: direction/forward
top level: pSB8C15
start: 3342
end: 3419
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2202888
display id: annotation2202888
version: 1
top level: pSB8C15
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2202888/range2202888/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2202888/range2202888/1
type: Range
persistent identity: range2202888
display id: range2202888
version: 1
direction: direction/reverse
top level: pSB8C15
start: 3235
end: 3461
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2203156
display id: annotation2203156
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203156/range2203156/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203156/range2203156/1
type: Range
persistent identity: range2203156
display id: range2203156
version: 1
direction: direction/forward
top level: pSB8C15
start: 881
end: 886
orientation: inline

VF2 forward primer binding site
type: SequenceAnnotation
persistent identity: annotation2203154
display id: annotation2203154
version: 1
top level: pSB8C15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203154/range2203154/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203154/range2203154/1
type: Range
persistent identity: range2203154
display id: range2203154
version: 1
direction: direction/forward
top level: pSB8C15
start: 3322
end: 3341
orientation: inline

VR reverse primer binding site
type: SequenceAnnotation
persistent identity: annotation2203046
display id: annotation2203046
version: 1
top level: pSB8C15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203046/range2203046/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203046/range2203046/1
type: Range
persistent identity: range2203046
display id: range2203046
version: 1
direction: direction/reverse
top level: pSB8C15
start: 116
end: 135
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2203160
display id: annotation2203160
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203160/range2203160/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203160/range2203160/1
type: Range
persistent identity: range2203160
display id: range2203160
version: 1
direction: direction/forward
top level: pSB8C15
start: 3281
end: 3321
orientation: inline

pSC101-ts ori
type: SequenceAnnotation
persistent identity: annotation2203153
display id: annotation2203153
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203153/range2203153/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203153/range2203153/1
type: Range
persistent identity: range2203153
display id: range2203153
version: 1
direction: direction/forward
top level: pSB8C15
start: 190
end: 2405
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation2203158
display id: annotation2203158
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203158/range2203158/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203158/range2203158/1
type: Range
persistent identity: range2203158
display id: range2203158
version: 1
direction: direction/forward
top level: pSB8C15
start: 136
end: 175
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2203162
display id: annotation2203162
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203162/range2203162/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203162/range2203162/1
type: Range
persistent identity: range2203162
display id: range2203162
version: 1
direction: direction/forward
top level: pSB8C15
start: 903
end: 908
orientation: inline

BBa_M36095
type: ComponentDefinition
persistent identity: BBa M36095
display id: BBa_M36095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S. elongatus cyanobacteria sensor test rig
creator: Joseph Shih
created: 2013-04-22T11:00:00Z
modified: 2015-05-08T01:14:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 10436
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: S. elongatus test vector for sensors. Has a cyanocitrus YFP coding sequence as a reporter. Comes with high copy number origin and kanamycin resistance
mutable notes: Redesigned by the BIOE44 teaching staff from pNS2-YFP.
mutable provenance: Source plasmid is pNS2-YFP. pNS2-YFP is a plasmid made by Rachel Hood from Dr. Dave Savage's lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: NS2 site #1
sequence annotation: cyanocitrus YFP
sequence annotation: student sensors go here
sequence annotation: RBS
sequence annotation: rrnB transcription terminator
sequence annotation: NS2 site #2
sequence annotation: KanR cassette
sequence annotation: E. coli ORI
sequence: https://synbiohub.org/public/igem/BBa_M36095_sequence/1

NS2 site #1
type: SequenceAnnotation
persistent identity: annotation2215810
display id: annotation2215810
version: 1
top level: BBa_M36095
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_M36095/annotation2215810/range2215810/1

https://synbiohub.org/public/igem/BBa_M36095/annotation2215810/range2215810/1
type: Range
persistent identity: range2215810
display id: range2215810
version: 1
direction: direction/forward
top level: BBa_M36095
start: 1
end: 1484
orientation: inline

cyanocitrus YFP
type: SequenceAnnotation
persistent identity: annotation2215813
display id: annotation2215813
version: 1
top level: BBa_M36095
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_M36095/annotation2215813/range2215813/1

https://synbiohub.org/public/igem/BBa_M36095/annotation2215813/range2215813/1
type: Range
persistent identity: range2215813
display id: range2215813
version: 1
direction: direction/forward
top level: BBa_M36095
start: 1767
end: 2482
orientation: inline

student sensors go here
type: SequenceAnnotation
persistent identity: annotation2215811
display id: annotation2215811
version: 1
top level: BBa_M36095
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36095/annotation2215811/range2215811/1

https://synbiohub.org/public/igem/BBa_M36095/annotation2215811/range2215811/1
type: Range
persistent identity: range2215811
display id: range2215811
version: 1
direction: direction/forward
top level: BBa_M36095
start: 1715
end: 1751
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2215812
display id: annotation2215812
version: 1
top level: BBa_M36095
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_M36095/annotation2215812/range2215812/1

https://synbiohub.org/public/igem/BBa_M36095/annotation2215812/range2215812/1
type: Range
persistent identity: range2215812
display id: range2215812
version: 1
direction: direction/forward
top level: BBa_M36095
start: 1752
end: 1766
orientation: inline

rrnB transcription terminator
type: SequenceAnnotation
persistent identity: annotation2215814
display id: annotation2215814
version: 1
top level: BBa_M36095
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_M36095/annotation2215814/range2215814/1

https://synbiohub.org/public/igem/BBa_M36095/annotation2215814/range2215814/1
type: Range
persistent identity: range2215814
display id: range2215814
version: 1
direction: direction/forward
top level: BBa_M36095
start: 2496
end: 3064
orientation: inline

NS2 site #2
type: SequenceAnnotation
persistent identity: annotation2215816
display id: annotation2215816
version: 1
top level: BBa_M36095
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_M36095/annotation2215816/range2215816/1

https://synbiohub.org/public/igem/BBa_M36095/annotation2215816/range2215816/1
type: Range
persistent identity: range2215816
display id: range2215816
version: 1
direction: direction/forward
top level: BBa_M36095
start: 4332
end: 5064
orientation: inline

KanR cassette
type: SequenceAnnotation
persistent identity: annotation2215815
display id: annotation2215815
version: 1
top level: BBa_M36095
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_M36095/annotation2215815/range2215815/1

https://synbiohub.org/public/igem/BBa_M36095/annotation2215815/range2215815/1
type: Range
persistent identity: range2215815
display id: range2215815
version: 1
direction: direction/reverse
top level: BBa_M36095
start: 3075
end: 4326
orientation: inline

E. coli ORI
type: SequenceAnnotation
persistent identity: annotation2215817
display id: annotation2215817
version: 1
top level: BBa_M36095
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36095/annotation2215817/range2215817/1

https://synbiohub.org/public/igem/BBa_M36095/annotation2215817/range2215817/1
type: Range
persistent identity: range2215817
display id: range2215817
version: 1
direction: direction/reverse
top level: BBa_M36095
start: 7982
end: 8842
orientation: inline

BBa_K289012
type: ComponentDefinition
persistent identity: BBa K289012
display id: BBa_K289012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBS-TII
creator: Nan Hou
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _393_
m user id: 0
owner id: 4781
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmide with a Ampicilin selecting marker and Multiple cloning site within the lacZ(selection marker) gene on it.
mutable notes: quickly selecting plasmide with lacZ marker on it.
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: EcoRI
sequence annotation: insertation site
sequence annotation: T3
sequence annotation: m13 forward Primer
sequence annotation: M13 reverse
sequence annotation: PstI
sequence annotation: XhoI
sequence annotation: XbaI
sequence annotation: T7
sequence: https://synbiohub.org/public/igem/BBa_K289012_sequence/1

EcoRI
type: SequenceAnnotation
persistent identity: annotation2061306
display id: annotation2061306
version: 1
top level: BBa_K289012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K289012/annotation2061306/range2061306/1

https://synbiohub.org/public/igem/BBa_K289012/annotation2061306/range2061306/1
type: Range
persistent identity: range2061306
display id: range2061306
version: 1
direction: direction/forward
top level: BBa_K289012
start: 102
end: 103
orientation: inline

insertation site
type: SequenceAnnotation
persistent identity: annotation2061376
display id: annotation2061376
version: 1
top level: BBa_K289012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K289012/annotation2061376/range2061376/1

https://synbiohub.org/public/igem/BBa_K289012/annotation2061376/range2061376/1
type: Range
persistent identity: range2061376
display id: range2061376
version: 1
direction: direction/forward
top level: BBa_K289012
start: 115
end: 116
orientation: inline

T3
type: SequenceAnnotation
persistent identity: annotation2061345
display id: annotation2061345
version: 1
top level: BBa_K289012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K289012/annotation2061345/range2061345/1

https://synbiohub.org/public/igem/BBa_K289012/annotation2061345/range2061345/1
type: Range
persistent identity: range2061345
display id: range2061345
version: 1
direction: direction/reverse
top level: BBa_K289012
start: 192
end: 213
orientation: inline

m13 forward Primer
type: SequenceAnnotation
persistent identity: annotation2061301
display id: annotation2061301
version: 1
top level: BBa_K289012
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K289012/annotation2061301/range2061301/1

https://synbiohub.org/public/igem/BBa_K289012/annotation2061301/range2061301/1
type: Range
persistent identity: range2061301
display id: range2061301
version: 1
direction: direction/forward
top level: BBa_K289012
start: 1
end: 17
orientation: inline

M13 reverse
type: SequenceAnnotation
persistent identity: annotation2061346
display id: annotation2061346
version: 1
top level: BBa_K289012
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K289012/annotation2061346/range2061346/1

https://synbiohub.org/public/igem/BBa_K289012/annotation2061346/range2061346/1
type: Range
persistent identity: range2061346
display id: range2061346
version: 1
direction: direction/reverse
top level: BBa_K289012
start: 232
end: 250
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2061307
display id: annotation2061307
version: 1
top level: BBa_K289012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K289012/annotation2061307/range2061307/1

https://synbiohub.org/public/igem/BBa_K289012/annotation2061307/range2061307/1
type: Range
persistent identity: range2061307
display id: range2061307
version: 1
direction: direction/forward
top level: BBa_K289012
start: 133
end: 134
orientation: inline

XhoI
type: SequenceAnnotation
persistent identity: annotation2061305
display id: annotation2061305
version: 1
top level: BBa_K289012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K289012/annotation2061305/range2061305/1

https://synbiohub.org/public/igem/BBa_K289012/annotation2061305/range2061305/1
type: Range
persistent identity: range2061305
display id: range2061305
version: 1
direction: direction/forward
top level: BBa_K289012
start: 69
end: 70
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2061344
display id: annotation2061344
version: 1
top level: BBa_K289012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K289012/annotation2061344/range2061344/1

https://synbiohub.org/public/igem/BBa_K289012/annotation2061344/range2061344/1
type: Range
persistent identity: range2061344
display id: range2061344
version: 1
direction: direction/forward
top level: BBa_K289012
start: 152
end: 153
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2061304
display id: annotation2061304
version: 1
top level: BBa_K289012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K289012/annotation2061304/range2061304/1

https://synbiohub.org/public/igem/BBa_K289012/annotation2061304/range2061304/1
type: Range
persistent identity: range2061304
display id: range2061304
version: 1
direction: direction/forward
top level: BBa_K289012
start: 26
end: 45
orientation: inline

Screening
type: ComponentDefinition
persistent identity: pSB1A10
display id: pSB1A10
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid v1.0
creator: Josh Michener, Jason Kelly
created: 2006-05-25T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: E0040 (EGFP)
sequence annotation: B0034
sequence annotation: barcode
sequence annotation: I0500 (pBAD/araC)
sequence annotation: suffix
sequence annotation: Hairpin
sequence annotation: prefix
sequence annotation: RNAse E site
sequence annotation: B0015 (Term)
sequence annotation: psb1a3
sequence annotation: Scar from BB suffix removal by I13450
sequence annotation: Scar from BB prefix removal by I13450
sequence annotation: spacer artifact from 13450
sequence annotation: E1010 (mRFP1)
sequence: https://synbiohub.org/public/igem/pSB1A10_sequence/1

E0040 (EGFP)
type: SequenceAnnotation
persistent identity: annotation1871317
display id: annotation1871317
version: 1
top level: Screening
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871317/range1871317/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871317/range1871317/1
type: Range
persistent identity: range1871317
display id: range1871317
version: 1
direction: direction/forward
top level: Screening
start: 4475
end: 5194
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1871316
display id: annotation1871316
version: 1
top level: Screening
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871316/range1871316/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871316/range1871316/1
type: Range
persistent identity: range1871316
display id: range1871316
version: 1
direction: direction/forward
top level: Screening
start: 4457
end: 4468
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1871310
display id: annotation1871310
version: 1
top level: Screening
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871310/range1871310/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871310/range1871310/1
type: Range
persistent identity: range1871310
display id: range1871310
version: 1
direction: direction/forward
top level: Screening
start: 801
end: 825
orientation: inline

I0500 (pBAD/araC)
type: SequenceAnnotation
persistent identity: annotation1871315
display id: annotation1871315
version: 1
top level: Screening
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871315/range1871315/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871315/range1871315/1
type: Range
persistent identity: range1871315
display id: range1871315
version: 1
direction: direction/forward
top level: Screening
start: 3239
end: 4448
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1871283
display id: annotation1871283
version: 1
top level: Screening
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871283/range1871283/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871283/range1871283/1
type: Range
persistent identity: range1871283
display id: range1871283
version: 1
direction: direction/forward
top level: Screening
start: 1
end: 21
orientation: inline

Hairpin
type: SequenceAnnotation
persistent identity: annotation1871307
display id: annotation1871307
version: 1
top level: Screening
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871307/range1871307/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871307/range1871307/1
type: Range
persistent identity: range1871307
display id: range1871307
version: 1
direction: direction/forward
top level: Screening
start: 59
end: 93
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1871320
display id: annotation1871320
version: 1
top level: Screening
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871320/range1871320/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871320/range1871320/1
type: Range
persistent identity: range1871320
display id: range1871320
version: 1
direction: direction/forward
top level: Screening
start: 5242
end: 5263
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1871318
display id: annotation1871318
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871318/range1871318/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871318/range1871318/1
type: Range
persistent identity: range1871318
display id: range1871318
version: 1
direction: direction/forward
top level: Screening
start: 5203
end: 5223
orientation: inline

B0015 (Term)
type: SequenceAnnotation
persistent identity: annotation1871311
display id: annotation1871311
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871311/range1871311/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871311/range1871311/1
type: Range
persistent identity: range1871311
display id: range1871311
version: 1
direction: direction/forward
top level: Screening
start: 834
end: 962
orientation: inline

psb1a3
type: SequenceAnnotation
persistent identity: annotation1871313
display id: annotation1871313
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871313/range1871313/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871313/range1871313/1
type: Range
persistent identity: range1871313
display id: range1871313
version: 1
direction: direction/forward
top level: Screening
start: 1045
end: 3157
orientation: inline

Scar from BB suffix removal by I13450
type: SequenceAnnotation
persistent identity: annotation1871312
display id: annotation1871312
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871312/range1871312/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871312/range1871312/1
type: Range
persistent identity: range1871312
display id: range1871312
version: 1
direction: direction/forward
top level: Screening
start: 963
end: 1044
orientation: inline

Scar from BB prefix removal by I13450
type: SequenceAnnotation
persistent identity: annotation1871314
display id: annotation1871314
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871314/range1871314/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871314/range1871314/1
type: Range
persistent identity: range1871314
display id: range1871314
version: 1
direction: direction/forward
top level: Screening
start: 3158
end: 3238
orientation: inline

spacer artifact from 13450
type: SequenceAnnotation
persistent identity: annotation1871319
display id: annotation1871319
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871319/range1871319/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871319/range1871319/1
type: Range
persistent identity: range1871319
display id: range1871319
version: 1
direction: direction/forward
top level: Screening
start: 5224
end: 5241
orientation: inline

E1010 (mRFP1)
type: SequenceAnnotation
persistent identity: annotation1871309
display id: annotation1871309
version: 1
top level: Screening
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871309/range1871309/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871309/range1871309/1
type: Range
persistent identity: range1871309
display id: range1871309
version: 1
direction: direction/forward
top level: Screening
start: 120
end: 800
orientation: inline

spacer artifact from 13450
type: SequenceAnnotation
persistent identity: annotation1871305
display id: annotation1871305
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871305/range1871305/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871305/range1871305/1
type: Range
persistent identity: range1871305
display id: range1871305
version: 1
direction: direction/forward
top level: Screening
start: 22
end: 37
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1871306
display id: annotation1871306
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871306/range1871306/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871306/range1871306/1
type: Range
persistent identity: range1871306
display id: range1871306
version: 1
direction: direction/forward
top level: Screening
start: 38
end: 58
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1871308
display id: annotation1871308
version: 1
top level: Screening
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871308/range1871308/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871308/range1871308/1
type: Range
persistent identity: range1871308
display id: range1871308
version: 1
direction: direction/forward
top level: Screening
start: 102
end: 113
orientation: inline

BBa_K2170301
type: ComponentDefinition
persistent identity: BBa K2170301
display id: BBa_K2170301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcDNA5 for genomic integration
creator: Max Mustermann
created: 2016-10-11T11:00:00Z
modified: 2017-03-17T05:09:37Z
discontinued: false
dominant: false
group u list: _2641_
m user id: 32573
owner id: 32573
owning group id: 9
status: status/Planning
bookmark: false
mutable description: genomic integration
mutable notes: none
mutable provenance: Chair for biological chemistry TUM
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K2170301_sequence/1

BBa_K390200
type: ComponentDefinition
persistent identity: BBa K390200
display id: BBa_K390200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A3_IntC - General Integration Plasmid with CmR
creator: Cody Tramp
created: 2010-08-02T11:00:00Z
modified: 2015-05-08T01:12:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _518_
m user id: 0
owner id: 6788
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: (coming soon)
mutable notes: (coming soon)
mutable provenance: (coming soon)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K390200_sequence/1

pSB1A3
type: ComponentDefinition
persistent identity: pSB1A3
display id: pSB1A3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Reshma Shetty & Tom Knight
created: 2004-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 52
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1A3-1 is a high copy number plasmid carrying ampicillin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). pSB1A3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region. A fully annotated Genbank file is attached below.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: terminator stem loop
sequence annotation: end of terminator
sequence annotation: E. coli his operon terminator
sequence annotation: rep(pMB1) (rev)
sequence annotation: VR
sequence annotation: ampR (rev)
sequence annotation: ORI
sequence annotation: prefix
sequence annotation: VF2
sequence annotation: suffix
sequence: https://synbiohub.org/public/igem/pSB1A3_sequence/1

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2119971
display id: annotation2119971
version: 1
top level: pSB1A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119971/range2119971/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119971/range2119971/1
type: Range
persistent identity: range2119971
display id: range2119971
version: 1
direction: direction/forward
top level: pSB1A3
start: 30
end: 64
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2119977
display id: annotation2119977
version: 1
top level: pSB1A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119977/range2119977/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119977/range2119977/1
type: Range
persistent identity: range2119977
display id: range2119977
version: 1
direction: direction/reverse
top level: pSB1A3
start: 2094
end: 2097
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2119970
display id: annotation2119970
version: 1
top level: pSB1A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119970/range2119970/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119970/range2119970/1
type: Range
persistent identity: range2119970
display id: range2119970
version: 1
direction: direction/forward
top level: pSB1A3
start: 22
end: 93
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2119973
display id: annotation2119973
version: 1
top level: pSB1A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119973/range2119973/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119973/range2119973/1
type: Range
persistent identity: range2119973
display id: range2119973
version: 1
direction: direction/reverse
top level: pSB1A3
start: 261
end: 875
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2119972
display id: annotation2119972
version: 1
top level: pSB1A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119972/range2119972/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119972/range2119972/1
type: Range
persistent identity: range2119972
display id: range2119972
version: 1
direction: direction/reverse
top level: pSB1A3
start: 157
end: 176
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2119978
display id: annotation2119978
version: 1
top level: pSB1A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119978/range2119978/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119978/range2119978/1
type: Range
persistent identity: range2119978
display id: range2119978
version: 1
direction: direction/reverse
top level: pSB1A3
start: 2107
end: 2126
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation2119975
display id: annotation2119975
version: 1
top level: pSB1A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119975/range2119975/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119975/range2119975/1
type: Range
persistent identity: range2119975
display id: range2119975
version: 1
direction: direction/reverse
top level: pSB1A3
start: 1035
end: 1895
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2119974
display id: annotation2119974
version: 1
top level: pSB1A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119974/range2119974/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119974/range2119974/1
type: Range
persistent identity: range2119974
display id: range2119974
version: 1
direction: direction/forward
top level: pSB1A3
start: 276
end: 276
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2119979
display id: annotation2119979
version: 1
top level: pSB1A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119979/range2119979/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119979/range2119979/1
type: Range
persistent identity: range2119979
display id: range2119979
version: 1
direction: direction/forward
top level: pSB1A3
start: 2136
end: 2157
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2119976
display id: annotation2119976
version: 1
top level: pSB1A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119976/range2119976/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119976/range2119976/1
type: Range
persistent identity: range2119976
display id: range2119976
version: 1
direction: direction/forward
top level: pSB1A3
start: 2018
end: 2037
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2119969
display id: annotation2119969
version: 1
top level: pSB1A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119969/range2119969/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119969/range2119969/1
type: Range
persistent identity: range2119969
display id: range2119969
version: 1
direction: direction/forward
top level: pSB1A3
start: 1
end: 21
orientation: inline

pSB4A3
type: ComponentDefinition
persistent identity: pSB4A3
display id: pSB4A3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4A3 (Replaced by pSB4A5)
creator: Reshma Shetty
created: 2004-06-28T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB4A3-1 is a low copy plasmid with ampicillin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) pSB4A3 has a terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into any DNA outside the MCS.The orientation of the pBLA promoter driving the AmpR gene is such that relatively little transcription should be coming into the MCS from upstream. A second terminator (E.coli His operon-derived) is downstream of the MCS, again insulating the vector from transcription reading out of the MCS. Ideally, future versions of standard biobrick vectors would have terminators bracketing the MCS that were 100% efficient in terminating transcription both into and out of the MCS region.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VF2
sequence annotation: end of terminator
sequence annotation: VR
sequence annotation: repA (rev)
sequence annotation: deletion of two bases
sequence annotation: prefix
sequence annotation: terminator stem loop
sequence annotation: AmpR (rev)
sequence annotation: (rev)
sequence annotation: E.coli His operon terminator
sequence annotation: rbs(rev)
sequence annotation: -10
sequence annotation: Terminator (rev)
sequence annotation: suffix
sequence annotation: RBS (rev)
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/pSB4A3_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1782550
display id: annotation1782550
version: 1
top level: pSB4A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782550/range1782550/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782550/range1782550/1
type: Range
persistent identity: range1782550
display id: range1782550
version: 1
direction: direction/forward
top level: pSB4A3
start: 3200
end: 3219
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1782530
display id: annotation1782530
version: 1
top level: pSB4A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782530/range1782530/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782530/range1782530/1
type: Range
persistent identity: range1782530
display id: range1782530
version: 1
direction: direction/reverse
top level: pSB4A3
start: 3276
end: 3279
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782542
display id: annotation1782542
version: 1
top level: pSB4A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782542/range1782542/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782542/range1782542/1
type: Range
persistent identity: range1782542
display id: range1782542
version: 1
direction: direction/reverse
top level: pSB4A3
start: 157
end: 176
orientation: inline

repA (rev)
type: SequenceAnnotation
persistent identity: annotation1782534
display id: annotation1782534
version: 1
top level: pSB4A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782534/range1782534/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782534/range1782534/1
type: Range
persistent identity: range1782534
display id: range1782534
version: 1
direction: direction/reverse
top level: pSB4A3
start: 384
end: 1334
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823771
display id: annotation1823771
version: 1
top level: pSB4A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB4A3/annotation1823771/range1823771/1

https://synbiohub.org/public/igem/pSB4A3/annotation1823771/range1823771/1
type: Range
persistent identity: range1823771
display id: range1823771
version: 1
direction: direction/forward
top level: pSB4A3
start: 34
end: 35
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1782531
display id: annotation1782531
version: 1
top level: pSB4A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782531/range1782531/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782531/range1782531/1
type: Range
persistent identity: range1782531
display id: range1782531
version: 1
direction: direction/forward
top level: pSB4A3
start: 3318
end: 3339
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824031
display id: annotation1824031
version: 1
top level: pSB4A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB4A3/annotation1824031/range1824031/1

https://synbiohub.org/public/igem/pSB4A3/annotation1824031/range1824031/1
type: Range
persistent identity: range1824031
display id: range1824031
version: 1
direction: direction/forward
top level: pSB4A3
start: 30
end: 64
orientation: inline

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation1782537
display id: annotation1782537
version: 1
top level: pSB4A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782537/range1782537/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782537/range1782537/1
type: Range
persistent identity: range1782537
display id: range1782537
version: 1
direction: direction/reverse
top level: pSB4A3
start: 2220
end: 3080
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824032
display id: annotation1824032
version: 1
top level: pSB4A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB4A3/annotation1824032/range1824032/1

https://synbiohub.org/public/igem/pSB4A3/annotation1824032/range1824032/1
type: Range
persistent identity: range1824032
display id: range1824032
version: 1
direction: direction/reverse
top level: pSB4A3
start: 3289
end: 3308
orientation: inline

(rev)
type: SequenceAnnotation
persistent identity: annotation1782536
display id: annotation1782536
version: 1
top level: pSB4A3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782536/range1782536/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782536/range1782536/1
type: Range
persistent identity: range1782536
display id: range1782536
version: 1
direction: direction/reverse
top level: pSB4A3
start: 1355
end: 1374
orientation: inline

E.coli His operon terminator
type: SequenceAnnotation
persistent identity: annotation1782533
display id: annotation1782533
version: 1
top level: pSB4A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782533/range1782533/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782533/range1782533/1
type: Range
persistent identity: range1782533
display id: range1782533
version: 1
direction: direction/forward
top level: pSB4A3
start: 22
end: 93
orientation: inline

rbs(rev)
type: SequenceAnnotation
persistent identity: annotation1782527
display id: annotation1782527
version: 1
top level: pSB4A3
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782527/range1782527/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782527/range1782527/1
type: Range
persistent identity: range1782527
display id: range1782527
version: 1
direction: direction/reverse
top level: pSB4A3
start: 3085
end: 3089
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1782528
display id: annotation1782528
version: 1
top level: pSB4A3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782528/range1782528/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782528/range1782528/1
type: Range
persistent identity: range1782528
display id: range1782528
version: 1
direction: direction/forward
top level: pSB4A3
start: 3098
end: 3103
orientation: inline

Terminator (rev)
type: SequenceAnnotation
persistent identity: annotation1782538
display id: annotation1782538
version: 1
top level: pSB4A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782538/range1782538/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782538/range1782538/1
type: Range
persistent identity: range1782538
display id: range1782538
version: 1
direction: direction/reverse
top level: pSB4A3
start: 2145
end: 2174
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1782532
display id: annotation1782532
version: 1
top level: pSB4A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782532/range1782532/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782532/range1782532/1
type: Range
persistent identity: range1782532
display id: range1782532
version: 1
direction: direction/forward
top level: pSB4A3
start: 1
end: 21
orientation: inline

RBS (rev)
type: SequenceAnnotation
persistent identity: annotation1782535
display id: annotation1782535
version: 1
top level: pSB4A3
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782535/range1782535/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782535/range1782535/1
type: Range
persistent identity: range1782535
display id: range1782535
version: 1
direction: direction/reverse
top level: pSB4A3
start: 1338
end: 1350
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1782529
display id: annotation1782529
version: 1
top level: pSB4A3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782529/range1782529/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782529/range1782529/1
type: Range
persistent identity: range1782529
display id: range1782529
version: 1
direction: direction/forward
top level: pSB4A3
start: 3124
end: 3129
orientation: inline

B. S. Vect
type: ComponentDefinition
persistent identity: BBa K606003
display id: BBa_K606003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Multi-host replicative plasmid, for B. Subtilis and E. Coli
creator: Cyrille Pauthenier
created: 2011-07-24T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: true
dominant: false
experience: experience/Issues
group u list: _778_
m user id: 0
owner id: 8998
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20). This plasmid is ideal for importing parts in Subtilis: all the cloning can be done in E. Coli, as well as the amplification. Once the plasmid is extracted, you can incorporate it directly in B. Subtilis using electroporation. This plasmid is Ampicilyn resistant in E. Coli, and is tetracycline resistant in both B. Subtilis and E. Coli. The biobrick BBa_J04450 is inserted between the E X and SP site, has a negative cloning control in E. Coli
mutable notes: The EcoRI site has to be eliminated and the Biobricks prefixes and suffixes were recovered
mutable provenance: The plasmid was obtained from Harald Putzer laboratory at the IBPC [http://www.ibpc.fr/UPR9073/equipe_HaraldJackie/AccueilJAP_HPGB.htm] . This is the fusion of the commercial plasmids pBC16-1 and pTZ19R via the site EcoRI. This site has been eliminated by the 2011 iGEM Paris Team by quick change mutagenesis, and the multicloning site was transformed into the biobrick standards.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Biobrick SP
sequence annotation: Replication origin for E. Coli
sequence annotation: pBC16-1 plasmid (B.S. plasmid)
sequence annotation: Biobrick EX
sequence annotation: LacZ alpha disabled by the cloning
sequence annotation: DNA: Major Replication origin OriU
sequence annotation: Beta lactamase peptide (ampicilin)
sequence annotation: Tetracycline resistance gene
sequence annotation: SYNPTZ19R
sequence annotation: pT7 promoter
sequence annotation: DNA minor replication origin OriL
sequence: https://synbiohub.org/public/igem/BBa_K606003_sequence/1

Biobrick SP
type: SequenceAnnotation
persistent identity: annotation2124971
display id: annotation2124971
version: 1
top level: B. S. Vect
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124971/range2124971/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124971/range2124971/1
type: Range
persistent identity: range2124971
display id: range2124971
version: 1
direction: direction/forward
top level: B. S. Vect
start: 5905
end: 5924
orientation: inline

Replication origin for E. Coli
type: SequenceAnnotation
persistent identity: annotation2124969
display id: annotation2124969
version: 1
top level: B. S. Vect
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124969/range2124969/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124969/range2124969/1
type: Range
persistent identity: range2124969
display id: range2124969
version: 1
direction: direction/forward
top level: B. S. Vect
start: 4867
end: 5469
orientation: inline

pBC16-1 plasmid (B.S. plasmid)
type: SequenceAnnotation
persistent identity: annotation2124962
display id: annotation2124962
version: 1
top level: B. S. Vect
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124962/range2124962/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124962/range2124962/1
type: Range
persistent identity: range2124962
display id: range2124962
version: 1
direction: direction/forward
top level: B. S. Vect
start: 22
end: 3072
orientation: inline

Biobrick EX
type: SequenceAnnotation
persistent identity: annotation2124961
display id: annotation2124961
version: 1
top level: B. S. Vect
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124961/range2124961/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124961/range2124961/1
type: Range
persistent identity: range2124961
display id: range2124961
version: 1
direction: direction/reverse
top level: B. S. Vect
start: 1
end: 21
orientation: inline

LacZ alpha disabled by the cloning
type: SequenceAnnotation
persistent identity: annotation2124966
display id: annotation2124966
version: 1
top level: B. S. Vect
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124966/range2124966/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124966/range2124966/1
type: Range
persistent identity: range2124966
display id: range2124966
version: 1
direction: direction/reverse
top level: B. S. Vect
start: 3076
end: 3244
orientation: inline

DNA: Major Replication origin OriU
type: SequenceAnnotation
persistent identity: annotation2124965
display id: annotation2124965
version: 1
top level: B. S. Vect
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124965/range2124965/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124965/range2124965/1
type: Range
persistent identity: range2124965
display id: range2124965
version: 1
direction: direction/forward
top level: B. S. Vect
start: 2813
end: 2838
orientation: inline

Beta lactamase peptide (ampicilin)
type: SequenceAnnotation
persistent identity: annotation2124968
display id: annotation2124968
version: 1
top level: B. S. Vect
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124968/range2124968/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124968/range2124968/1
type: Range
persistent identity: range2124968
display id: range2124968
version: 1
direction: direction/reverse
top level: B. S. Vect
start: 3835
end: 4692
orientation: inline

Tetracycline resistance gene
type: SequenceAnnotation
persistent identity: annotation2124963
display id: annotation2124963
version: 1
top level: B. S. Vect
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124963/range2124963/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124963/range2124963/1
type: Range
persistent identity: range2124963
display id: range2124963
version: 1
direction: direction/forward
top level: B. S. Vect
start: 80
end: 1468
orientation: inline

SYNPTZ19R
type: SequenceAnnotation
persistent identity: annotation2124967
display id: annotation2124967
version: 1
top level: B. S. Vect
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124967/range2124967/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124967/range2124967/1
type: Range
persistent identity: range2124967
display id: range2124967
version: 1
direction: direction/reverse
top level: B. S. Vect
start: 3078
end: 5904
orientation: inline

pT7 promoter
type: SequenceAnnotation
persistent identity: annotation2124970
display id: annotation2124970
version: 1
top level: B. S. Vect
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124970/range2124970/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124970/range2124970/1
type: Range
persistent identity: range2124970
display id: range2124970
version: 1
direction: direction/forward
top level: B. S. Vect
start: 5871
end: 5893
orientation: inline

DNA minor replication origin OriL
type: SequenceAnnotation
persistent identity: annotation2124964
display id: annotation2124964
version: 1
top level: B. S. Vect
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124964/range2124964/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124964/range2124964/1
type: Range
persistent identity: range2124964
display id: range2124964
version: 1
direction: direction/forward
top level: B. S. Vect
start: 2120
end: 2398
orientation: inline

pSBBs1C
type: ComponentDefinition
persistent identity: BBa K823021
display id: BBa_K823021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs1C-lacZ (lacZ reporter vector for B. subtilis)
creator: Jara Radeck
created: 2012-08-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a backbone vector for Bacillus subtilis. It integrates at the amyE locus. It has a Ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: This reporter vector can be used with the normal BioBrick assembly strategies.
mutable provenance: This vector is the Bacillus vector pAC6 brought into the iGEM BioBrick standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: cat
sequence annotation: amyE'
sequence annotation: lacZ
sequence annotation: bla
sequence annotation: 'amyE
sequence: https://synbiohub.org/public/igem/BBa_K823021_sequence/1

cat
type: SequenceAnnotation
persistent identity: annotation2179606
display id: annotation2179606
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179606/range2179606/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179606/range2179606/1
type: Range
persistent identity: range2179606
display id: range2179606
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 8813
end: 9463
orientation: inline

amyE'
type: SequenceAnnotation
persistent identity: annotation2179603
display id: annotation2179603
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179603/range2179603/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179603/range2179603/1
type: Range
persistent identity: range2179603
display id: range2179603
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 3165
end: 3698
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation2179602
display id: annotation2179602
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179602/range2179602/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179602/range2179602/1
type: Range
persistent identity: range2179602
display id: range2179602
version: 1
direction: direction/forward
top level: pSBBs1C
start: 49
end: 3112
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2179604
display id: annotation2179604
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179604/range2179604/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179604/range2179604/1
type: Range
persistent identity: range2179604
display id: range2179604
version: 1
direction: direction/forward
top level: pSBBs1C
start: 4023
end: 4883
orientation: inline

'amyE
type: SequenceAnnotation
persistent identity: annotation2179605
display id: annotation2179605
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179605/range2179605/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179605/range2179605/1
type: Range
persistent identity: range2179605
display id: range2179605
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 7402
end: 8229
orientation: inline

BBa_J72118
type: ComponentDefinition
persistent identity: BBa J72118
display id: BBa_J72118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p15A assembly vector, pBjh1601CA
creator: Jin Huh
created: 2012-01-12T12:00:00Z
modified: 2015-05-08T01:08:27Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 3025
owning group id: 171
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p15A assembly vector, CmR and AmpR
mutable notes: p15A origin, used in layered assembly of BglBrick parts
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72118_sequence/1

pSB4A5
type: ComponentDefinition
persistent identity: pSB4A5
display id: pSB4A5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-26T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4A5 is a low copy plasmid with ampicillin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
mutable provenance: BBa_I51001, pSC101 origin and a new version of ampR stripped of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0043
component: BBa_B0054
component: BBa_G00102
component: BBa_B0042
component: BBa_G00100
component: BBa_B0062
component: BBa_G00000
component: BBa_I50042
component: BBa_B0055
component: BBa_G00001
component: BBa_P1002
component: BBa_G0004
component: BBa_B0044
component: BBa_G0003
component: BBa_B0053
component: BBa_G0002
sequence annotation: BBa_G00102
sequence annotation: BBa_G0004
sequence annotation: BBa_B0043
sequence annotation: BBa_B0054
sequence annotation: BBa_B0062
sequence annotation: BBa_I50042
sequence annotation: BBa_G0003
sequence annotation: BBa_P1002
sequence annotation: BBa_G00001
sequence annotation: BBa_B0055
sequence annotation: BBa_G00000
sequence annotation: BBa_G0002
sequence annotation: BBa_B0042
sequence annotation: BBa_B0044
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence: https://synbiohub.org/public/igem/pSB4A5_sequence/1

BBa_B0043
type: Component
persistent identity: component2236391
display id: component2236391
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0054
type: Component
persistent identity: component2236332
display id: component2236332
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00102
type: Component
persistent identity: component2236334
display id: component2236334
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component2236329
display id: component2236329
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00100
type: Component
persistent identity: component2236376
display id: component2236376
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0062
type: Component
persistent identity: component2236336
display id: component2236336
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G00000
type: Component
persistent identity: component2236396
display id: component2236396
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_I50042
type: Component
persistent identity: component2236365
display id: component2236365
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0055
type: Component
persistent identity: component2236379
display id: component2236379
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00001
type: Component
persistent identity: component2236317
display id: component2236317
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_P1002
type: Component
persistent identity: component2236371
display id: component2236371
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_G0004
type: Component
persistent identity: component2236372
display id: component2236372
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0042
type: Component
persistent identity: component2236386
display id: component2236386
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component2236322
display id: component2236322
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G0003
type: Component
persistent identity: component2236337
display id: component2236337
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0053
type: Component
persistent identity: component2236374
display id: component2236374
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0002
type: Component
persistent identity: component2236366
display id: component2236366
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236334
display id: annotation2236334
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236334/range2236334/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4A5/annotation2236334/range2236334/1
type: Range
persistent identity: range2236334
display id: range2236334
version: 1
direction: direction/reverse
top level: pSB4A5
start: 116
end: 135
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236372
display id: annotation2236372
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236372/range2236372/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4A5/annotation2236372/range2236372/1
type: Range
persistent identity: range2236372
display id: range2236372
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3172
end: 3178
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236391
display id: annotation2236391
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236391/range2236391/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4A5/annotation2236391/range2236391/1
type: Range
persistent identity: range2236391
display id: range2236391
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3361
end: 3373
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236332
display id: annotation2236332
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236332/range2236332/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4A5/annotation2236332/range2236332/1
type: Range
persistent identity: range2236332
display id: range2236332
version: 1
direction: direction/reverse
top level: pSB4A5
start: 47
end: 115
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236336
display id: annotation2236336
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236336/range2236336/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4A5/annotation2236336/range2236336/1
type: Range
persistent identity: range2236336
display id: range2236336
version: 1
direction: direction/reverse
top level: pSB4A5
start: 136
end: 176
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236365
display id: annotation2236365
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236365/range2236365/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4A5/annotation2236365/range2236365/1
type: Range
persistent identity: range2236365
display id: range2236365
version: 1
direction: direction/reverse
top level: pSB4A5
start: 184
end: 2220
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236337
display id: annotation2236337
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236337/range2236337/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4A5/annotation2236337/range2236337/1
type: Range
persistent identity: range2236337
display id: range2236337
version: 1
direction: direction/reverse
top level: pSB4A5
start: 177
end: 183
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2236371
display id: annotation2236371
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236371/range2236371/1
component: BBa_P1002

https://synbiohub.org/public/igem/pSB4A5/annotation2236371/range2236371/1
type: Range
persistent identity: range2236371
display id: range2236371
version: 1
direction: direction/reverse
top level: pSB4A5
start: 2229
end: 3171
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236317
display id: annotation2236317
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236317/range2236317/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4A5/annotation2236317/range2236317/1
type: Range
persistent identity: range2236317
display id: range2236317
version: 1
direction: direction/reverse
top level: pSB4A5
start: 1
end: 21
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236379
display id: annotation2236379
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236379/range2236379/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4A5/annotation2236379/range2236379/1
type: Range
persistent identity: range2236379
display id: range2236379
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3271
end: 3348
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236396
display id: annotation2236396
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236396/range2236396/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4A5/annotation2236396/range2236396/1
type: Range
persistent identity: range2236396
display id: range2236396
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3374
end: 3395
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236366
display id: annotation2236366
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236366/range2236366/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4A5/annotation2236366/range2236366/1
type: Range
persistent identity: range2236366
display id: range2236366
version: 1
direction: direction/reverse
top level: pSB4A5
start: 2221
end: 2228
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236386
display id: annotation2236386
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236386/range2236386/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4A5/annotation2236386/range2236386/1
type: Range
persistent identity: range2236386
display id: range2236386
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3349
end: 3360
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236322
display id: annotation2236322
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236322/range2236322/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4A5/annotation2236322/range2236322/1
type: Range
persistent identity: range2236322
display id: range2236322
version: 1
direction: direction/reverse
top level: pSB4A5
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236329
display id: annotation2236329
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236329/range2236329/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4A5/annotation2236329/range2236329/1
type: Range
persistent identity: range2236329
display id: range2236329
version: 1
direction: direction/reverse
top level: pSB4A5
start: 35
end: 46
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236374
display id: annotation2236374
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236374/range2236374/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4A5/annotation2236374/range2236374/1
type: Range
persistent identity: range2236374
display id: range2236374
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3179
end: 3250
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236376
display id: annotation2236376
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236376/range2236376/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4A5/annotation2236376/range2236376/1
type: Range
persistent identity: range2236376
display id: range2236376
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3251
end: 3270
orientation: inline

BBa_K1137006
type: ComponentDefinition
persistent identity: BBa K1137006
display id: BBa_K1137006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB6A3
creator: Matthew Deyell
created: 2013-10-03T11:00:00Z
modified: 2015-05-08T01:09:21Z
discontinued: false
dominant: false
group u list: _1449_
m user id: 0
owner id: 15098
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB6A3 - contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with ColE1 replicon derived from pBR322 with a copy number of approximately 40 per cell. The cat gene has been replaced with ampR cassette conveying ampicillin resistance.
mutable notes: Designed to be stable with pSB9S1, pSB10K1, and pSB3C7
mutable provenance: Original sequence used was pSB1C3, replacing the ori and resistance cassette with those from pET
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Prefix
sequence annotation: f1 ori
sequence annotation: Terminator
sequence annotation: ampR
sequence annotation: ColE1
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K1137006_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation2367917
display id: annotation2367917
version: 1
top level: BBa_K1137006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1137006/annotation2367917/range2367917/1

https://synbiohub.org/public/igem/BBa_K1137006/annotation2367917/range2367917/1
type: Range
persistent identity: range2367917
display id: range2367917
version: 1
direction: direction/forward
top level: BBa_K1137006
start: 3544
end: 3564
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation2367912
display id: annotation2367912
version: 1
top level: BBa_K1137006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1137006/annotation2367912/range2367912/1

https://synbiohub.org/public/igem/BBa_K1137006/annotation2367912/range2367912/1
type: Range
persistent identity: range2367912
display id: range2367912
version: 1
direction: direction/forward
top level: BBa_K1137006
start: 65
end: 512
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2367920
display id: annotation2367920
version: 1
top level: BBa_K1137006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137006/annotation2367920/range2367920/1

https://synbiohub.org/public/igem/BBa_K1137006/annotation2367920/range2367920/1
type: Range
persistent identity: range2367920
display id: range2367920
version: 1
direction: direction/forward
top level: BBa_K1137006
start: 4656
end: 4725
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2367913
display id: annotation2367913
version: 1
top level: BBa_K1137006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1137006/annotation2367913/range2367913/1

https://synbiohub.org/public/igem/BBa_K1137006/annotation2367913/range2367913/1
type: Range
persistent identity: range2367913
display id: range2367913
version: 1
direction: direction/reverse
top level: BBa_K1137006
start: 638
end: 1498
orientation: inline

ColE1
type: SequenceAnnotation
persistent identity: annotation2367914
display id: annotation2367914
version: 1
top level: BBa_K1137006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1137006/annotation2367914/range2367914/1

https://synbiohub.org/public/igem/BBa_K1137006/annotation2367914/range2367914/1
type: Range
persistent identity: range2367914
display id: range2367914
version: 1
direction: direction/reverse
top level: BBa_K1137006
start: 1593
end: 2323
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2367916
display id: annotation2367916
version: 1
top level: BBa_K1137006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137006/annotation2367916/range2367916/1

https://synbiohub.org/public/igem/BBa_K1137006/annotation2367916/range2367916/1
type: Range
persistent identity: range2367916
display id: range2367916
version: 1
direction: direction/forward
top level: BBa_K1137006
start: 3502
end: 3542
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2367919
display id: annotation2367919
version: 1
top level: BBa_K1137006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1137006/annotation2367919/range2367919/1

https://synbiohub.org/public/igem/BBa_K1137006/annotation2367919/range2367919/1
type: Range
persistent identity: range2367919
display id: range2367919
version: 1
direction: direction/forward
top level: BBa_K1137006
start: 4636
end: 4655
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2367918
display id: annotation2367918
version: 1
top level: BBa_K1137006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1137006/annotation2367918/range2367918/1

https://synbiohub.org/public/igem/BBa_K1137006/annotation2367918/range2367918/1
type: Range
persistent identity: range2367918
display id: range2367918
version: 1
direction: direction/forward
top level: BBa_K1137006
start: 3792
end: 4469
orientation: inline

BBa_J176123
type: ComponentDefinition
persistent identity: BBa J176123
display id: BBa_J176123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcDNA3.1+ hygro
creator: Brady Laughlin
created: 2011-10-21T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pcDNA3.1+ hygro/ΔpCMV, based on pcDNA3.1+; no promoter; amp, hygro
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: SV40
sequence annotation: DD122B
sequence annotation: f1 origin
sequence annotation: pUC origin
sequence annotation: CMV promoter remnant
sequence annotation: BGH
sequence annotation: Hygromycin resistance
sequence annotation: bla
sequence annotation: Ampicillin resistance
sequence annotation: SpeI NotI XbaI
sequence: https://synbiohub.org/public/igem/BBa_J176123_sequence/1

SV40
type: SequenceAnnotation
persistent identity: annotation2167173
display id: annotation2167173
version: 1
top level: BBa_J176123
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167173/range2167173/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167173/range2167173/1
type: Range
persistent identity: range2167173
display id: range2167173
version: 1
direction: direction/forward
top level: BBa_J176123
start: 2628
end: 2758
orientation: inline

DD122B
type: SequenceAnnotation
persistent identity: annotation2167204
display id: annotation2167204
version: 1
top level: BBa_J176123
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167204/range2167204/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167204/range2167204/1
type: Range
persistent identity: range2167204
display id: range2167204
version: 1
direction: direction/forward
top level: BBa_J176123
start: 186
end: 203
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2167170
display id: annotation2167170
version: 1
top level: BBa_J176123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167170/range2167170/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167170/range2167170/1
type: Range
persistent identity: range2167170
display id: range2167170
version: 1
direction: direction/forward
top level: BBa_J176123
start: 612
end: 1040
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2167174
display id: annotation2167174
version: 1
top level: BBa_J176123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167174/range2167174/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167174/range2167174/1
type: Range
persistent identity: range2167174
display id: range2167174
version: 1
direction: direction/reverse
top level: BBa_J176123
start: 3141
end: 3811
orientation: inline

CMV promoter remnant
type: SequenceAnnotation
persistent identity: annotation2167166
display id: annotation2167166
version: 1
top level: BBa_J176123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167166/range2167166/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167166/range2167166/1
type: Range
persistent identity: range2167166
display id: range2167166
version: 1
direction: direction/forward
top level: BBa_J176123
start: 232
end: 249
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2167168
display id: annotation2167168
version: 1
top level: BBa_J176123
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167168/range2167168/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167168/range2167168/1
type: Range
persistent identity: range2167168
display id: range2167168
version: 1
direction: direction/reverse
top level: BBa_J176123
start: 336
end: 353
orientation: inline

Hygromycin resistance
type: SequenceAnnotation
persistent identity: annotation2167172
display id: annotation2167172
version: 1
top level: BBa_J176123
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167172/range2167172/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167172/range2167172/1
type: Range
persistent identity: range2167172
display id: range2167172
version: 1
direction: direction/forward
top level: BBa_J176123
start: 1438
end: 2468
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2167176
display id: annotation2167176
version: 1
top level: BBa_J176123
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167176/range2167176/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167176/range2167176/1
type: Range
persistent identity: range2167176
display id: range2167176
version: 1
direction: direction/reverse
top level: BBa_J176123
start: 4816
end: 4915
orientation: inline

SV40
type: SequenceAnnotation
persistent identity: annotation2167171
display id: annotation2167171
version: 1
top level: BBa_J176123
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167171/range2167171/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167171/range2167171/1
type: Range
persistent identity: range2167171
display id: range2167171
version: 1
direction: direction/forward
top level: BBa_J176123
start: 1045
end: 1388
orientation: inline

Ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation2167175
display id: annotation2167175
version: 1
top level: BBa_J176123
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167175/range2167175/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167175/range2167175/1
type: Range
persistent identity: range2167175
display id: range2167175
version: 1
direction: direction/reverse
top level: BBa_J176123
start: 3956
end: 4816
orientation: inline

SpeI NotI XbaI
type: SequenceAnnotation
persistent identity: annotation2167167
display id: annotation2167167
version: 1
top level: BBa_J176123
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167167/range2167167/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167167/range2167167/1
type: Range
persistent identity: range2167167
display id: range2167167
version: 1
direction: direction/forward
top level: BBa_J176123
start: 250
end: 324
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2167169
display id: annotation2167169
version: 1
top level: BBa_J176123
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176123/annotation2167169/range2167169/1

https://synbiohub.org/public/igem/BBa_J176123/annotation2167169/range2167169/1
type: Range
persistent identity: range2167169
display id: range2167169
version: 1
direction: direction/forward
top level: BBa_J176123
start: 342
end: 566
orientation: inline

BBa_J119048
type: ComponentDefinition
persistent identity: BBa J119048
display id: BBa_J119048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4A8
creator: A. Malcolm Campbell and Todd Eckdahl
created: 2012-01-23T12:00:00Z
modified: 2015-08-31T04:08:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Insulated low copy vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. Uses I50042 low copy pSC101 replication origin.
mutable notes: BsaI site removed from amplicillin resistance gene. Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.
mutable provenance: de novo DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR primer
sequence annotation: B0025 terminator
sequence annotation: I50042 low copy pSC101 origin
sequence annotation: stuffer
sequence annotation: Ampicillin resistance gene
sequence annotation: VF2 primer
sequence annotation: Suffix
sequence annotation: B0010
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_J119048_sequence/1

VR primer
type: SequenceAnnotation
persistent identity: annotation2169706
display id: annotation2169706
version: 1
top level: BBa_J119048
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119048/annotation2169706/range2169706/1

https://synbiohub.org/public/igem/BBa_J119048/annotation2169706/range2169706/1
type: Range
persistent identity: range2169706
display id: range2169706
version: 1
direction: direction/reverse
top level: BBa_J119048
start: 208
end: 227
orientation: inline

B0025 terminator
type: SequenceAnnotation
persistent identity: annotation2169710
display id: annotation2169710
version: 1
top level: BBa_J119048
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J119048/annotation2169710/range2169710/1

https://synbiohub.org/public/igem/BBa_J119048/annotation2169710/range2169710/1
type: Range
persistent identity: range2169710
display id: range2169710
version: 1
direction: direction/forward
top level: BBa_J119048
start: 22
end: 205
orientation: inline

I50042 low copy pSC101 origin
type: SequenceAnnotation
persistent identity: annotation2169709
display id: annotation2169709
version: 1
top level: BBa_J119048
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119048/annotation2169709/range2169709/1

https://synbiohub.org/public/igem/BBa_J119048/annotation2169709/range2169709/1
type: Range
persistent identity: range2169709
display id: range2169709
version: 1
direction: direction/forward
top level: BBa_J119048
start: 314
end: 2350
orientation: inline

stuffer
type: SequenceAnnotation
persistent identity: annotation2169712
display id: annotation2169712
version: 1
top level: BBa_J119048
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119048/annotation2169712/range2169712/1

https://synbiohub.org/public/igem/BBa_J119048/annotation2169712/range2169712/1
type: Range
persistent identity: range2169712
display id: range2169712
version: 1
direction: direction/forward
top level: BBa_J119048
start: 3651
end: 3662
orientation: inline

Ampicillin resistance gene
type: SequenceAnnotation
persistent identity: annotation2169708
display id: annotation2169708
version: 1
top level: BBa_J119048
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J119048/annotation2169708/range2169708/1

https://synbiohub.org/public/igem/BBa_J119048/annotation2169708/range2169708/1
type: Range
persistent identity: range2169708
display id: range2169708
version: 1
direction: direction/reverse
top level: BBa_J119048
start: 2508
end: 3368
orientation: inline

VF2 primer
type: SequenceAnnotation
persistent identity: annotation2169707
display id: annotation2169707
version: 1
top level: BBa_J119048
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119048/annotation2169707/range2169707/1

https://synbiohub.org/public/igem/BBa_J119048/annotation2169707/range2169707/1
type: Range
persistent identity: range2169707
display id: range2169707
version: 1
direction: direction/forward
top level: BBa_J119048
start: 3491
end: 3510
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2169704
display id: annotation2169704
version: 1
top level: BBa_J119048
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119048/annotation2169704/range2169704/1

https://synbiohub.org/public/igem/BBa_J119048/annotation2169704/range2169704/1
type: Range
persistent identity: range2169704
display id: range2169704
version: 1
direction: direction/forward
top level: BBa_J119048
start: 1
end: 21
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation2169711
display id: annotation2169711
version: 1
top level: BBa_J119048
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J119048/annotation2169711/range2169711/1

https://synbiohub.org/public/igem/BBa_J119048/annotation2169711/range2169711/1
type: Range
persistent identity: range2169711
display id: range2169711
version: 1
direction: direction/forward
top level: BBa_J119048
start: 3589
end: 3628
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2169705
display id: annotation2169705
version: 1
top level: BBa_J119048
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119048/annotation2169705/range2169705/1

https://synbiohub.org/public/igem/BBa_J119048/annotation2169705/range2169705/1
type: Range
persistent identity: range2169705
display id: range2169705
version: 1
direction: direction/forward
top level: BBa_J119048
start: 3629
end: 3650
orientation: inline

BBa_K560015
type: ComponentDefinition
persistent identity: BBa K560015
display id: BBa_K560015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BsaI Golden Gate Assembly No.4
creator: Zhang Chao
created: 2011-10-25T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
group u list: _728_
m user id: 0
owner id: 8553
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The forth vector of the Golden gate series (adapted from psb1c3) This vector expedites golden gate cloning with BBa_K560012, BBa_K560013 and BBa_K560014. To make this set of vector compatible with the standard biobricks, we have maintained PstI and XbaI recognition sites. Flanking these two sites are the cleavage and recognition sites of BsaI, a kind of Type IIS restriction endonuclease. This kind of restriction endonucleases cleaves outside their recognition sites thus creating different sticky ends. If you want to assemble four biobricks at a time, you can put the biobricks in order into corresponding vector by the digestion of PstI and Xbal. Then mix these assembled vectors and a backbone (PFUS4 from TALEN golden gate kit). Under the effect of ligase and Bsal, these biobricks will have sticky ends nose to tails and ligate in order in a single reaction.
mutable notes: No
mutable provenance: Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Research, 2011, Vol. 39, No. 12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BsaI
sequence annotation: XbaI
sequence annotation: PstI
sequence annotation: Suffix
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_K560015_sequence/1

BsaI
type: SequenceAnnotation
persistent identity: annotation2160728
display id: annotation2160728
version: 1
top level: BBa_K560015
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560015/annotation2160728/range2160728/1

https://synbiohub.org/public/igem/BBa_K560015/annotation2160728/range2160728/1
type: Range
persistent identity: range2160728
display id: range2160728
version: 1
direction: direction/forward
top level: BBa_K560015
start: 14
end: 19
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2160731
display id: annotation2160731
version: 1
top level: BBa_K560015
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560015/annotation2160731/range2160731/1

https://synbiohub.org/public/igem/BBa_K560015/annotation2160731/range2160731/1
type: Range
persistent identity: range2160731
display id: range2160731
version: 1
direction: direction/forward
top level: BBa_K560015
start: 2073
end: 2078
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2160727
display id: annotation2160727
version: 1
top level: BBa_K560015
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560015/annotation2160727/range2160727/1

https://synbiohub.org/public/igem/BBa_K560015/annotation2160727/range2160727/1
type: Range
persistent identity: range2160727
display id: range2160727
version: 1
direction: direction/forward
top level: BBa_K560015
start: 3
end: 8
orientation: inline

BsaI
type: SequenceAnnotation
persistent identity: annotation2160730
display id: annotation2160730
version: 1
top level: BBa_K560015
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560015/annotation2160730/range2160730/1

https://synbiohub.org/public/igem/BBa_K560015/annotation2160730/range2160730/1
type: Range
persistent identity: range2160730
display id: range2160730
version: 1
direction: direction/forward
top level: BBa_K560015
start: 2062
end: 2067
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2160729
display id: annotation2160729
version: 1
top level: BBa_K560015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K560015/annotation2160729/range2160729/1

https://synbiohub.org/public/igem/BBa_K560015/annotation2160729/range2160729/1
type: Range
persistent identity: range2160729
display id: range2160729
version: 1
direction: direction/forward
top level: BBa_K560015
start: 2058
end: 2080
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2160588
display id: annotation2160588
version: 1
top level: BBa_K560015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K560015/annotation2160588/range2160588/1

https://synbiohub.org/public/igem/BBa_K560015/annotation2160588/range2160588/1
type: Range
persistent identity: range2160588
display id: range2160588
version: 1
direction: direction/forward
top level: BBa_K560015
start: 1
end: 24
orientation: inline

BBa_K864010
type: ComponentDefinition
persistent identity: BBa K864010
display id: BBa_K864010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864010_sequence/1

BBa_K245004
type: ComponentDefinition
persistent identity: BBa K245004
display id: BBa_K245004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No Description --
creator: Ota Fekonja
created: 2009-09-15T11:00:00Z
modified: 2015-05-08T01:11:38Z
discontinued: true
dominant: false
experience: experience/None
group u list: _337_
m user id: 0
owner id: 4948
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: BB-NIC-II-HisN originates from BioBrick vector pSB1A2 but contains modified prefix and suffix according to assembly standard 37. T7 promoter is included in prefix and T7 terminator in suffix. There are four new inner restriction sites added in multiple-cloning site (NgoMIV, AgeI-prefix; XmaI/BspEI-suffix) which enable extension of part cloned into pSB1A30 with S/G on N-terminal or T/G on C-terminal; extension of linker and creating a friendly scar during parts fusion.
mutable notes: BioBrick vector pSB1A2 was first cut with EcoRI/PstI. An insert containing modified prefix and suffix (according to assembly standard 37) was ligated in between. Subsequently a ccdB domain was inserted between AgeI and XmaI restriction sites.
mutable provenance: The source is BioBrick vector pSB1A2; modified prefix and suffix sequences are of synthetic origin; ccdB originates from part BBa_P1010.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: XmaI
sequence annotation: BspEI
sequence annotation: T7 promoter
sequence annotation: SpeI
sequence annotation: T7 terminator
sequence annotation: amp promoter
sequence annotation: modified sufix
sequence annotation: PstI
sequence annotation: NgoMIV
sequence annotation: amp resistance marker
sequence annotation: rbs
sequence annotation: XbaI
sequence annotation: modified prefix
sequence annotation: NotI
sequence annotation: start
sequence annotation: His tag
sequence annotation: stop
sequence annotation: pBR233 origin
sequence annotation: lacO regulatory site
sequence annotation: AgeI
sequence annotation: EcoRI
sequence: https://synbiohub.org/public/igem/BBa_K245004_sequence/1

XmaI
type: SequenceAnnotation
persistent identity: annotation2021199
display id: annotation2021199
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021199/range2021199/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021199/range2021199/1
type: Range
persistent identity: range2021199
display id: range2021199
version: 1
direction: direction/forward
top level: BBa_K245004
start: 1
end: 6
orientation: inline

BspEI
type: SequenceAnnotation
persistent identity: annotation2021200
display id: annotation2021200
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021200/range2021200/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021200/range2021200/1
type: Range
persistent identity: range2021200
display id: range2021200
version: 1
direction: direction/forward
top level: BBa_K245004
start: 7
end: 12
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2021212
display id: annotation2021212
version: 1
top level: BBa_K245004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021212/range2021212/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021212/range2021212/1
type: Range
persistent identity: range2021212
display id: range2021212
version: 1
direction: direction/forward
top level: BBa_K245004
start: 2133
end: 2151
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2021201
display id: annotation2021201
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021201/range2021201/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021201/range2021201/1
type: Range
persistent identity: range2021201
display id: range2021201
version: 1
direction: direction/forward
top level: BBa_K245004
start: 13
end: 18
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2021203
display id: annotation2021203
version: 1
top level: BBa_K245004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021203/range2021203/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021203/range2021203/1
type: Range
persistent identity: range2021203
display id: range2021203
version: 1
direction: direction/forward
top level: BBa_K245004
start: 22
end: 69
orientation: inline

amp promoter
type: SequenceAnnotation
persistent identity: annotation2021208
display id: annotation2021208
version: 1
top level: BBa_K245004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021208/range2021208/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021208/range2021208/1
type: Range
persistent identity: range2021208
display id: range2021208
version: 1
direction: direction/reverse
top level: BBa_K245004
start: 1920
end: 1948
orientation: inline

modified sufix
type: SequenceAnnotation
persistent identity: annotation2021198
display id: annotation2021198
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021198/range2021198/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021198/range2021198/1
type: Range
persistent identity: range2021198
display id: range2021198
version: 1
direction: direction/forward
top level: BBa_K245004
start: 1
end: 82
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2021205
display id: annotation2021205
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021205/range2021205/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021205/range2021205/1
type: Range
persistent identity: range2021205
display id: range2021205
version: 1
direction: direction/forward
top level: BBa_K245004
start: 77
end: 82
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2021218
display id: annotation2021218
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021218/range2021218/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021218/range2021218/1
type: Range
persistent identity: range2021218
display id: range2021218
version: 1
direction: direction/forward
top level: BBa_K245004
start: 2250
end: 2255
orientation: inline

amp resistance marker
type: SequenceAnnotation
persistent identity: annotation2021207
display id: annotation2021207
version: 1
top level: BBa_K245004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021207/range2021207/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021207/range2021207/1
type: Range
persistent identity: range2021207
display id: range2021207
version: 1
direction: direction/reverse
top level: BBa_K245004
start: 1018
end: 1878
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2021214
display id: annotation2021214
version: 1
top level: BBa_K245004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021214/range2021214/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021214/range2021214/1
type: Range
persistent identity: range2021214
display id: range2021214
version: 1
direction: direction/forward
top level: BBa_K245004
start: 2208
end: 2214
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2021217
display id: annotation2021217
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021217/range2021217/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021217/range2021217/1
type: Range
persistent identity: range2021217
display id: range2021217
version: 1
direction: direction/forward
top level: BBa_K245004
start: 2244
end: 2249
orientation: inline

modified prefix
type: SequenceAnnotation
persistent identity: annotation2021209
display id: annotation2021209
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021209/range2021209/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021209/range2021209/1
type: Range
persistent identity: range2021209
display id: range2021209
version: 1
direction: direction/forward
top level: BBa_K245004
start: 2119
end: 2261
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation2021211
display id: annotation2021211
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021211/range2021211/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021211/range2021211/1
type: Range
persistent identity: range2021211
display id: range2021211
version: 1
direction: direction/forward
top level: BBa_K245004
start: 2125
end: 2132
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2021215
display id: annotation2021215
version: 1
top level: BBa_K245004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021215/range2021215/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021215/range2021215/1
type: Range
persistent identity: range2021215
display id: range2021215
version: 1
direction: direction/forward
top level: BBa_K245004
start: 2220
end: 2222
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2021216
display id: annotation2021216
version: 1
top level: BBa_K245004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021216/range2021216/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021216/range2021216/1
type: Range
persistent identity: range2021216
display id: range2021216
version: 1
direction: direction/forward
top level: BBa_K245004
start: 2226
end: 2243
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation2021204
display id: annotation2021204
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021204/range2021204/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021204/range2021204/1
type: Range
persistent identity: range2021204
display id: range2021204
version: 1
direction: direction/forward
top level: BBa_K245004
start: 70
end: 77
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2021202
display id: annotation2021202
version: 1
top level: BBa_K245004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021202/range2021202/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021202/range2021202/1
type: Range
persistent identity: range2021202
display id: range2021202
version: 1
direction: direction/forward
top level: BBa_K245004
start: 19
end: 21
orientation: inline

pBR233 origin
type: SequenceAnnotation
persistent identity: annotation2021206
display id: annotation2021206
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021206/range2021206/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021206/range2021206/1
type: Range
persistent identity: range2021206
display id: range2021206
version: 1
direction: direction/reverse
top level: BBa_K245004
start: 244
end: 863
orientation: inline

lacO regulatory site
type: SequenceAnnotation
persistent identity: annotation2021213
display id: annotation2021213
version: 1
top level: BBa_K245004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021213/range2021213/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021213/range2021213/1
type: Range
persistent identity: range2021213
display id: range2021213
version: 1
direction: direction/forward
top level: BBa_K245004
start: 2151
end: 2178
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2021197
display id: annotation2021197
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021197/range2021197/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021197/range2021197/1
type: Range
persistent identity: range2021197
display id: range2021197
version: 1
direction: direction/forward
top level: BBa_K245004
start: 2256
end: 2261
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2021210
display id: annotation2021210
version: 1
top level: BBa_K245004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K245004/annotation2021210/range2021210/1

https://synbiohub.org/public/igem/BBa_K245004/annotation2021210/range2021210/1
type: Range
persistent identity: range2021210
display id: range2021210
version: 1
direction: direction/forward
top level: BBa_K245004
start: 2119
end: 2124
orientation: inline

LT
type: ComponentDefinition
persistent identity: BBa K2144101
display id: BBa_K2144101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Vector containing Hisx6 and Sortase tag LPETGG
creator: Sigrun Stulz
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T01:15:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2614_
m user id: 31510
owner id: 31510
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This vector is constructed to simplify the usage of the transpeptidase Sortase A for future iGEM teams. Following a BamHI restriction site we constructed a synthetic sequence consisting of three main elements: 1) A linker (GSSS) to allow for limited mobility of the attached peptides and facilitate correct folding 2) A Sortase A tag (LPETGG) that is used to conjugate the proteins to poly-glycines 3) A standard Hisx6 tag commonly used for Immobilized Metal Affinity Chromatography (IMAC) This sequence follows a BamHI restriction site which can be used by teams to clone in whichever peptide they would like to conjugate using Sortase A.
mutable notes: BamHI is a very convenient restriction enzyme for our application as its restriction site will code for a glycine and a serine, both of which are often used in linkers and tend to be unproblematic in such sequences.
mutable provenance: The plasmid was constructed using the iGEM-supplied pSB1C3 and a synthetically produced sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: stop
sequence annotation: stem_loop
sequence annotation: Stop
sequence annotation: Sortase A Tag
sequence annotation: Hisx6
sequence annotation: BBa_R0010
sequence annotation: -10
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: CmR
sequence annotation: polya
sequence annotation: -35
sequence annotation: conserved
sequence annotation: T7 TE
sequence annotation: CAP
sequence annotation: ori
sequence annotation: barcode
sequence annotation: start
sequence annotation: BBa_B0012
sequence annotation: LacI
sequence: https://synbiohub.org/public/igem/BBa_K2144101_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2509050
display id: annotation2509050
version: 1
top level: LT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509050/range2509050/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509050/range2509050/1
type: Range
persistent identity: range2509050
display id: range2509050
version: 1
direction: direction/forward
top level: LT
start: 1062
end: 1062
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2509046
display id: annotation2509046
version: 1
top level: LT
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509046/range2509046/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509046/range2509046/1
type: Range
persistent identity: range2509046
display id: range2509046
version: 1
direction: direction/forward
top level: LT
start: 952
end: 995
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2509054
display id: annotation2509054
version: 1
top level: LT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509054/range2509054/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509054/range2509054/1
type: Range
persistent identity: range2509054
display id: range2509054
version: 1
direction: direction/forward
top level: LT
start: 2095
end: 2097
orientation: inline

Sortase A Tag
type: SequenceAnnotation
persistent identity: annotation2509053
display id: annotation2509053
version: 1
top level: LT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509053/range2509053/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509053/range2509053/1
type: Range
persistent identity: range2509053
display id: range2509053
version: 1
direction: direction/forward
top level: LT
start: 2059
end: 2076
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2509052
display id: annotation2509052
version: 1
top level: LT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509052/range2509052/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509052/range2509052/1
type: Range
persistent identity: range2509052
display id: range2509052
version: 1
direction: direction/forward
top level: LT
start: 2077
end: 2094
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2509035
display id: annotation2509035
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509035/range2509035/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509035/range2509035/1
type: Range
persistent identity: range2509035
display id: range2509035
version: 1
direction: direction/forward
top level: LT
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2509038
display id: annotation2509038
version: 1
top level: LT
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509038/range2509038/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509038/range2509038/1
type: Range
persistent identity: range2509038
display id: range2509038
version: 1
direction: direction/forward
top level: LT
start: 161
end: 166
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2509041
display id: annotation2509041
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509041/range2509041/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509041/range2509041/1
type: Range
persistent identity: range2509041
display id: range2509041
version: 1
direction: direction/forward
top level: LT
start: 209
end: 220
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2509045
display id: annotation2509045
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509045/range2509045/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509045/range2509045/1
type: Range
persistent identity: range2509045
display id: range2509045
version: 1
direction: direction/forward
top level: LT
start: 941
end: 1020
orientation: inline

CmR
type: SequenceAnnotation
persistent identity: annotation2509051
display id: annotation2509051
version: 1
top level: LT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509051/range2509051/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509051/range2509051/1
type: Range
persistent identity: range2509051
display id: range2509051
version: 1
direction: direction/forward
top level: LT
start: 1140
end: 1799
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2509049
display id: annotation2509049
version: 1
top level: LT
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509049/range2509049/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509049/range2509049/1
type: Range
persistent identity: range2509049
display id: range2509049
version: 1
direction: direction/forward
top level: LT
start: 1056
end: 1069
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2509037
display id: annotation2509037
version: 1
top level: LT
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509037/range2509037/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509037/range2509037/1
type: Range
persistent identity: range2509037
display id: range2509037
version: 1
direction: direction/forward
top level: LT
start: 137
end: 142
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2509042
display id: annotation2509042
version: 1
top level: LT
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509042/range2509042/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509042/range2509042/1
type: Range
persistent identity: range2509042
display id: range2509042
version: 1
direction: direction/forward
top level: LT
start: 213
end: 216
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2509048
display id: annotation2509048
version: 1
top level: LT
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509048/range2509048/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509048/range2509048/1
type: Range
persistent identity: range2509048
display id: range2509048
version: 1
direction: direction/forward
top level: LT
start: 1036
end: 1055
orientation: inline

CAP
type: SequenceAnnotation
persistent identity: annotation2509036
display id: annotation2509036
version: 1
top level: LT
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509036/range2509036/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509036/range2509036/1
type: Range
persistent identity: range2509036
display id: range2509036
version: 1
direction: direction/forward
top level: LT
start: 89
end: 126
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2509043
display id: annotation2509043
version: 1
top level: LT
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509043/range2509043/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509043/range2509043/1
type: Range
persistent identity: range2509043
display id: range2509043
version: 1
direction: direction/forward
top level: LT
start: 254
end: 842
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation2509044
display id: annotation2509044
version: 1
top level: LT
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509044/range2509044/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509044/range2509044/1
type: Range
persistent identity: range2509044
display id: range2509044
version: 1
direction: direction/forward
top level: LT
start: 908
end: 932
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2509040
display id: annotation2509040
version: 1
top level: LT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509040/range2509040/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509040/range2509040/1
type: Range
persistent identity: range2509040
display id: range2509040
version: 1
direction: direction/forward
top level: LT
start: 173
end: 173
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2509047
display id: annotation2509047
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509047/range2509047/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509047/range2509047/1
type: Range
persistent identity: range2509047
display id: range2509047
version: 1
direction: direction/forward
top level: LT
start: 1029
end: 1069
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation2509039
display id: annotation2509039
version: 1
top level: LT
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509039/range2509039/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509039/range2509039/1
type: Range
persistent identity: range2509039
display id: range2509039
version: 1
direction: direction/forward
top level: LT
start: 166
end: 200
orientation: inline

BBa_J72154
type: ComponentDefinition
persistent identity: BBa J72154
display id: BBa_J72154
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kanamycin + chloramphenicol resistant p15A vector (2ab vector)
creator: Josh Kittleson
created: 2012-08-11T11:00:00Z
modified: 2015-05-08T01:08:28Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Referred to internally as pBjh1601KC
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72154_sequence/1

BBa_K1033201
type: ComponentDefinition
persistent identity: BBa K1033201
display id: BBa_K1033201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance
creator: Viktor Blomkvist
created: 2013-08-22T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
group u list: _1340_
m user id: 0
owner id: 17571
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: E. coli Lactobacilli shuttle vector pSBLb4C15 is a shuttle vector containing the ori pSH71 originating from the plasmid pJP059 which was taken from Lactococcus lactis. It has a red insert.
mutable notes: Had to make sure it was compatible with biobrick standard 10
mutable provenance: Ori in pEL4C15 replaced with ori from pJP059. Chloramphenicol resistance replaced with erythromycin resistance.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

BBa_K1178001
type: ComponentDefinition
persistent identity: BBa K1178001
display id: BBa_K1178001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid Backbone pSB1C95 for RFC 95 Open Sequence Initiative
creator: Razan Alnahhas
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1491_
m user id: 0
owner id: 11540
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Is the same pSB1C3 backbone but includes homing endonuclease sites before the Biobrick prefix and after the Biobrick suffix.
mutable notes: None
mutable provenance: pSB1C3 from the parts kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: I CeuI Site
sequence annotation: pMB1 Origin
sequence annotation: BioBrick Suffix
sequence annotation: CAM Resistance CDS
sequence annotation: I SceI Site
sequence annotation: BioBrick Prefix
sequence: https://synbiohub.org/public/igem/BBa_K1178001_sequence/1

I CeuI Site
type: SequenceAnnotation
persistent identity: annotation2346868
display id: annotation2346868
version: 1
top level: BBa_K1178001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1178001/annotation2346868/range2346868/1

https://synbiohub.org/public/igem/BBa_K1178001/annotation2346868/range2346868/1
type: Range
persistent identity: range2346868
display id: range2346868
version: 1
direction: direction/forward
top level: BBa_K1178001
start: 22
end: 48
orientation: inline

pMB1 Origin
type: SequenceAnnotation
persistent identity: annotation2346870
display id: annotation2346870
version: 1
top level: BBa_K1178001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1178001/annotation2346870/range2346870/1

https://synbiohub.org/public/igem/BBa_K1178001/annotation2346870/range2346870/1
type: Range
persistent identity: range2346870
display id: range2346870
version: 1
direction: direction/reverse
top level: BBa_K1178001
start: 287
end: 902
orientation: inline

BioBrick Suffix
type: SequenceAnnotation
persistent identity: annotation2346866
display id: annotation2346866
version: 1
top level: BBa_K1178001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1178001/annotation2346866/range2346866/1

https://synbiohub.org/public/igem/BBa_K1178001/annotation2346866/range2346866/1
type: Range
persistent identity: range2346866
display id: range2346866
version: 1
direction: direction/forward
top level: BBa_K1178001
start: 1
end: 21
orientation: inline

CAM Resistance CDS
type: SequenceAnnotation
persistent identity: annotation2346881
display id: annotation2346881
version: 1
top level: BBa_K1178001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1178001/annotation2346881/range2346881/1

https://synbiohub.org/public/igem/BBa_K1178001/annotation2346881/range2346881/1
type: Range
persistent identity: range2346881
display id: range2346881
version: 1
direction: direction/forward
top level: BBa_K1178001
start: 1187
end: 1846
orientation: inline

I SceI Site
type: SequenceAnnotation
persistent identity: annotation2346882
display id: annotation2346882
version: 1
top level: BBa_K1178001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1178001/annotation2346882/range2346882/1

https://synbiohub.org/public/igem/BBa_K1178001/annotation2346882/range2346882/1
type: Range
persistent identity: range2346882
display id: range2346882
version: 1
direction: direction/forward
top level: BBa_K1178001
start: 2078
end: 2095
orientation: inline

BioBrick Prefix
type: SequenceAnnotation
persistent identity: annotation2346883
display id: annotation2346883
version: 1
top level: BBa_K1178001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1178001/annotation2346883/range2346883/1

https://synbiohub.org/public/igem/BBa_K1178001/annotation2346883/range2346883/1
type: Range
persistent identity: range2346883
display id: range2346883
version: 1
direction: direction/forward
top level: BBa_K1178001
start: 2096
end: 2115
orientation: inline

BBa_K1085014
type: ComponentDefinition
persistent identity: BBa K1085014
display id: BBa_K1085014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1AC3-HySp: Integrational backbone into B. subtilis amyE locus with IPTG inducible promoter
creator: S. P. van Kessel
created: 2013-09-10T11:00:00Z
modified: 2015-06-08T04:01:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 4206
owner id: 16152
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: We used the amyE intergrational backbone form Munich 2012 (BBa_K823023) that had no promoter. We've added the Hy_Spank IPTG inducable promoter from Rudner's Lab 2004. The Hy_Spank promoter is right in front of the prefix so any biobrick can be inserted in this backbone.
mutable notes: In pDR111 the Hy_spank promoter and the lacI gene are orientated in the same direction. In order to have the Hy_spank promoter right in front of the prefix we turend the promoter around.
mutable provenance: iGEM Munich 2012 (Rudner Lab 2004) and Rudner's Lab 2004 (pDR111)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: amp
sequence annotation: lacO
sequence annotation: amyE
sequence annotation: Hy_Spank
sequence annotation: prefix
sequence annotation: -35
sequence annotation: Term. (-12.8 kcals)
sequence annotation: lacI
sequence annotation: Term. (-14.4 kcals)
sequence annotation: cat
sequence annotation: suffix
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1085014_sequence/1

amp
type: SequenceAnnotation
persistent identity: annotation2338706
display id: annotation2338706
version: 1
top level: BBa_K1085014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338706/range2338706/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338706/range2338706/1
type: Range
persistent identity: range2338706
display id: range2338706
version: 1
direction: direction/reverse
top level: BBa_K1085014
start: 4361
end: 5221
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation2338708
display id: annotation2338708
version: 1
top level: BBa_K1085014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338708/range2338708/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338708/range2338708/1
type: Range
persistent identity: range2338708
display id: range2338708
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 7497
end: 7516
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation2338709
display id: annotation2338709
version: 1
top level: BBa_K1085014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338709/range2338709/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338709/range2338709/1
type: Range
persistent identity: range2338709
display id: range2338709
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 7568
end: 7588
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2338704
display id: annotation2338704
version: 1
top level: BBa_K1085014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338704/range2338704/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338704/range2338704/1
type: Range
persistent identity: range2338704
display id: range2338704
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 5546
end: 6059
orientation: inline

Hy_Spank
type: SequenceAnnotation
persistent identity: annotation2338702
display id: annotation2338702
version: 1
top level: BBa_K1085014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338702/range2338702/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338702/range2338702/1
type: Range
persistent identity: range2338702
display id: range2338702
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 7497
end: 7588
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2338713
display id: annotation2338713
version: 1
top level: BBa_K1085014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338713/range2338713/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338713/range2338713/1
type: Range
persistent identity: range2338713
display id: range2338713
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 7604
end: 7625
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2338711
display id: annotation2338711
version: 1
top level: BBa_K1085014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338711/range2338711/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338711/range2338711/1
type: Range
persistent identity: range2338711
display id: range2338711
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 7533
end: 7533
orientation: inline

Term. (-12.8 kcals)
type: SequenceAnnotation
persistent identity: annotation2339050
display id: annotation2339050
version: 1
top level: BBa_K1085014
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2339050/range2339050/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2339050/range2339050/1
type: Range
persistent identity: range2339050
display id: range2339050
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 7435
end: 7462
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2338705
display id: annotation2338705
version: 1
top level: BBa_K1085014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338705/range2338705/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338705/range2338705/1
type: Range
persistent identity: range2338705
display id: range2338705
version: 1
direction: direction/reverse
top level: BBa_K1085014
start: 6096
end: 7178
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2338703
display id: annotation2338703
version: 1
top level: BBa_K1085014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338703/range2338703/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338703/range2338703/1
type: Range
persistent identity: range2338703
display id: range2338703
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 1379
end: 2407
orientation: inline

Term. (-14.4 kcals)
type: SequenceAnnotation
persistent identity: annotation2339027
display id: annotation2339027
version: 1
top level: BBa_K1085014
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2339027/range2339027/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2339027/range2339027/1
type: Range
persistent identity: range2339027
display id: range2339027
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 314
end: 350
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2338707
display id: annotation2338707
version: 1
top level: BBa_K1085014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338707/range2338707/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338707/range2338707/1
type: Range
persistent identity: range2338707
display id: range2338707
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 358
end: 1008
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2338712
display id: annotation2338712
version: 1
top level: BBa_K1085014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338712/range2338712/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338712/range2338712/1
type: Range
persistent identity: range2338712
display id: range2338712
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 1
end: 21
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2338710
display id: annotation2338710
version: 1
top level: BBa_K1085014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1085014/annotation2338710/range2338710/1

https://synbiohub.org/public/igem/BBa_K1085014/annotation2338710/range2338710/1
type: Range
persistent identity: range2338710
display id: range2338710
version: 1
direction: direction/forward
top level: BBa_K1085014
start: 7558
end: 7558
orientation: inline

BBa_K260008
type: ComponentDefinition
persistent identity: BBa K260008
display id: BBa_K260008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMA-RQ-@CC2FosMB-10000bp
creator: Kaj Bernhardt
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _356_
m user id: 0
owner id: 1072
owning group id: 57
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This plasmid backbone is based on pMA-RQ from Geneart. It has BioBrick prefix and suffix flanked on both sides by 50 bp of homology arms for insertion of the contained BioBrick into the 10000 locus of pCC2FosMB (BBa_K260001) by recombineering. The pMA-RQ-@CC2FosMB-10000bp backbone is available with BBa_K260015, which is composed of promoter-FRT-dhfr. If the PvuII fragment from BBa_K260008-BBa_K260015 is transferred by recombineering to the the 10000 locus of BBa_K260001-BBa_K260016, the intervening sequence between the FRT sites of BBa_K260015 and BBa_K260016 on the BBa_K260001 backbone will be 10,000 bp exactly. These plasmid backbones are similar and all allow recombineering-mediated transfer of the contained BioBrick (typically BBa_K260015) to the pCC2FosMB backbone BBa_K2600001. They differ in the homology arms, which determines the locus of insertion into pCC2FosMB. The bp numbers denote final inter-FRT distance as described above: BBa_K260004: pMA-CC2FosMB-00500bp BBa_K260005: pMA-CC2FosMB-01000bp BBa_K260006: pMA-CC2FosMB-02000bp BBa_K260007: pMA-CC2FosMB-05000bp BBa_K260008: pMA-RQ-CC2FosMB-10000bp
mutable notes: The 50 bp homologies were designed to delete 100 bp of BBa_K260001-BBa_K260016 and put the FRT site of BBa_K260015 (if used with the present backbone BBa_K260008) at a defined distance from the FRT site of BBa_K260016.
mutable provenance: Geneart plasmid pMA-RQ, with 2x 50 bp from pCC2FosMB K260001.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: PvuII site
sequence annotation: AmpR
sequence annotation: ColE1 origin
sequence annotation: prefix
sequence annotation: BBa_K260001 (pCC2FosMB) 5' homology FRT-10000bp
sequence annotation: suffix
sequence annotation: BBa_K260001 (pCC2FosMB) 3' homology FRT-10000bp
sequence: https://synbiohub.org/public/igem/BBa_K260008_sequence/1

PvuII site
type: SequenceAnnotation
persistent identity: annotation2049430
display id: annotation2049430
version: 1
top level: BBa_K260008
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K260008/annotation2049430/range2049430/1

https://synbiohub.org/public/igem/BBa_K260008/annotation2049430/range2049430/1
type: Range
persistent identity: range2049430
display id: range2049430
version: 1
direction: direction/forward
top level: BBa_K260008
start: 2451
end: 2456
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2049435
display id: annotation2049435
version: 1
top level: BBa_K260008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K260008/annotation2049435/range2049435/1

https://synbiohub.org/public/igem/BBa_K260008/annotation2049435/range2049435/1
type: Range
persistent identity: range2049435
display id: range2049435
version: 1
direction: direction/reverse
top level: BBa_K260008
start: 1087
end: 1947
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation2049439
display id: annotation2049439
version: 1
top level: BBa_K260008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260008/annotation2049439/range2049439/1

https://synbiohub.org/public/igem/BBa_K260008/annotation2049439/range2049439/1
type: Range
persistent identity: range2049439
display id: range2049439
version: 1
direction: direction/reverse
top level: BBa_K260008
start: 272
end: 939
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2049449
display id: annotation2049449
version: 1
top level: BBa_K260008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260008/annotation2049449/range2049449/1

https://synbiohub.org/public/igem/BBa_K260008/annotation2049449/range2049449/1
type: Range
persistent identity: range2049449
display id: range2049449
version: 1
direction: direction/forward
top level: BBa_K260008
start: 2527
end: 2548
orientation: inline

PvuII site
type: SequenceAnnotation
persistent identity: annotation2049429
display id: annotation2049429
version: 1
top level: BBa_K260008
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K260008/annotation2049429/range2049429/1

https://synbiohub.org/public/igem/BBa_K260008/annotation2049429/range2049429/1
type: Range
persistent identity: range2049429
display id: range2049429
version: 1
direction: direction/forward
top level: BBa_K260008
start: 92
end: 97
orientation: inline

BBa_K260001 (pCC2FosMB) 5' homology FRT-10000bp
type: SequenceAnnotation
persistent identity: annotation2049444
display id: annotation2049444
version: 1
top level: BBa_K260008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260008/annotation2049444/range2049444/1

https://synbiohub.org/public/igem/BBa_K260008/annotation2049444/range2049444/1
type: Range
persistent identity: range2049444
display id: range2049444
version: 1
direction: direction/forward
top level: BBa_K260008
start: 2457
end: 2526
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2049418
display id: annotation2049418
version: 1
top level: BBa_K260008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K260008/annotation2049418/range2049418/1

https://synbiohub.org/public/igem/BBa_K260008/annotation2049418/range2049418/1
type: Range
persistent identity: range2049418
display id: range2049418
version: 1
direction: direction/forward
top level: BBa_K260008
start: 1
end: 21
orientation: inline

BBa_K260001 (pCC2FosMB) 3' homology FRT-10000bp
type: SequenceAnnotation
persistent identity: annotation2049419
display id: annotation2049419
version: 1
top level: BBa_K260008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K260008/annotation2049419/range2049419/1

https://synbiohub.org/public/igem/BBa_K260008/annotation2049419/range2049419/1
type: Range
persistent identity: range2049419
display id: range2049419
version: 1
direction: direction/forward
top level: BBa_K260008
start: 22
end: 91
orientation: inline

BBa_J18966
type: ComponentDefinition
persistent identity: BBa J18966
display id: BBa_J18966
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSS3K2
creator: Sean Sleight
created: 2013-06-17T11:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2480
owning group id: 172
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Vector with pSS Prefix B and Suffix C, confers kanamycin resistance, p15a origin of replication, constitutively expresses luxR and araC.
mutable notes: Inserted luxR and araC to be constitutively expressed in the reverse direction on the vector. Removed restriction sites in prefix and suffix to improve assembly success rate.
mutable provenance: Modified pGA3K3 from 2011 University of Washington iGEM team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J18966_sequence/1

BBa_J176171
type: ComponentDefinition
persistent identity: BBa J176171
display id: BBa_J176171
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kozak-XbaI-NLS-6His-stop
creator: Karmella Haynes
created: 2013-04-18T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mammalian expression vector (without a promoter) designed to add a eukaryotic RBS (Kozak) upstream (in-frame) and a nuclear localization tag, 6-histidine tag, and stop codon downstream (in-frame) of a protein coding insert. This method only works for Silver Standard (RFC 23) inserts.
mutable notes: * A Kozak-XbaI-NLS-6His-stop with SpeI sticky overhangs was inserted into the XbaI site. * Sequencing data confirms proper orientation of the inserted part. * XbaI site can accept any Silver Standard (RFC 23) protein coding region that is cut with XbaI/SpeI. * Single-cut vectors can easily re-ligate: It is highly recommended that the user dephosphorylates the XbaI-cut vector prior to ligation. * Insertion of a XbaI/SpeI insert is non-directional: It is high recommended that the user selects at least 3 colonies after ligation check (vi sequencing or some other method) for proper orientation of the insert.
mutable provenance: Built from BBa_J176122
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: f1 origin
sequence annotation: BGH polyA
sequence annotation: A - Stop - S - stop
sequence annotation: NLS
sequence annotation: Kozak
sequence annotation: bla promoter
sequence annotation: MCS: SpeI BstXI EcoRI PstI EcoRV BstXI NotI XhoI
sequence annotation: Ampicillin R
sequence annotation: BGH reverse
sequence annotation: SV40 promoter/ origin
sequence annotation: Puromycin R
sequence annotation: 6-His
sequence annotation: DD123 B
sequence annotation: XbaI
sequence annotation: SV40 polyA
sequence annotation: DD122 B
sequence annotation: pCMV remnant
sequence: https://synbiohub.org/public/igem/BBa_J176171_sequence/1

f1 origin
type: SequenceAnnotation
persistent identity: annotation2215320
display id: annotation2215320
version: 1
top level: BBa_J176171
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215320/range2215320/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215320/range2215320/1
type: Range
persistent identity: range2215320
display id: range2215320
version: 1
direction: direction/forward
top level: BBa_J176171
start: 694
end: 1122
orientation: inline

BGH polyA
type: SequenceAnnotation
persistent identity: annotation2215319
display id: annotation2215319
version: 1
top level: BBa_J176171
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215319/range2215319/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215319/range2215319/1
type: Range
persistent identity: range2215319
display id: range2215319
version: 1
direction: direction/forward
top level: BBa_J176171
start: 424
end: 648
orientation: inline

A - Stop - S - stop
type: SequenceAnnotation
persistent identity: annotation2215316
display id: annotation2215316
version: 1
top level: BBa_J176171
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215316/range2215316/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215316/range2215316/1
type: Range
persistent identity: range2215316
display id: range2215316
version: 1
direction: direction/forward
top level: BBa_J176171
start: 374
end: 386
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2215314
display id: annotation2215314
version: 1
top level: BBa_J176171
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215314/range2215314/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215314/range2215314/1
type: Range
persistent identity: range2215314
display id: range2215314
version: 1
direction: direction/forward
top level: BBa_J176171
start: 335
end: 355
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2215312
display id: annotation2215312
version: 1
top level: BBa_J176171
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215312/range2215312/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215312/range2215312/1
type: Range
persistent identity: range2215312
display id: range2215312
version: 1
direction: direction/forward
top level: BBa_J176171
start: 311
end: 328
orientation: inline

bla promoter
type: SequenceAnnotation
persistent identity: annotation2215325
display id: annotation2215325
version: 1
top level: BBa_J176171
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215325/range2215325/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215325/range2215325/1
type: Range
persistent identity: range2215325
display id: range2215325
version: 1
direction: direction/reverse
top level: BBa_J176171
start: 4476
end: 4574
orientation: inline

MCS: SpeI BstXI EcoRI PstI EcoRV BstXI NotI XhoI
type: SequenceAnnotation
persistent identity: annotation2215311
display id: annotation2215311
version: 1
top level: BBa_J176171
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215311/range2215311/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215311/range2215311/1
type: Range
persistent identity: range2215311
display id: range2215311
version: 1
direction: direction/forward
top level: BBa_J176171
start: 250
end: 304
orientation: inline

Ampicillin R
type: SequenceAnnotation
persistent identity: annotation2215324
display id: annotation2215324
version: 1
top level: BBa_J176171
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215324/range2215324/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215324/range2215324/1
type: Range
persistent identity: range2215324
display id: range2215324
version: 1
direction: direction/reverse
top level: BBa_J176171
start: 3615
end: 4475
orientation: inline

BGH reverse
type: SequenceAnnotation
persistent identity: annotation2215317
display id: annotation2215317
version: 1
top level: BBa_J176171
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215317/range2215317/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215317/range2215317/1
type: Range
persistent identity: range2215317
display id: range2215317
version: 1
direction: direction/reverse
top level: BBa_J176171
start: 418
end: 435
orientation: inline

SV40 promoter/ origin
type: SequenceAnnotation
persistent identity: annotation2215321
display id: annotation2215321
version: 1
top level: BBa_J176171
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215321/range2215321/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215321/range2215321/1
type: Range
persistent identity: range2215321
display id: range2215321
version: 1
direction: direction/forward
top level: BBa_J176171
start: 1127
end: 1471
orientation: inline

Puromycin R
type: SequenceAnnotation
persistent identity: annotation2215322
display id: annotation2215322
version: 1
top level: BBa_J176171
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215322/range2215322/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215322/range2215322/1
type: Range
persistent identity: range2215322
display id: range2215322
version: 1
direction: direction/forward
top level: BBa_J176171
start: 1475
end: 2074
orientation: inline

6-His
type: SequenceAnnotation
persistent identity: annotation2215315
display id: annotation2215315
version: 1
top level: BBa_J176171
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215315/range2215315/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215315/range2215315/1
type: Range
persistent identity: range2215315
display id: range2215315
version: 1
direction: direction/forward
top level: BBa_J176171
start: 356
end: 373
orientation: inline

DD123 B
type: SequenceAnnotation
persistent identity: annotation2215318
display id: annotation2215318
version: 1
top level: BBa_J176171
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215318/range2215318/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215318/range2215318/1
type: Range
persistent identity: range2215318
display id: range2215318
version: 1
direction: direction/reverse
top level: BBa_J176171
start: 426
end: 444
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2215313
display id: annotation2215313
version: 1
top level: BBa_J176171
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215313/range2215313/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215313/range2215313/1
type: Range
persistent identity: range2215313
display id: range2215313
version: 1
direction: direction/forward
top level: BBa_J176171
start: 329
end: 334
orientation: inline

SV40 polyA
type: SequenceAnnotation
persistent identity: annotation2215323
display id: annotation2215323
version: 1
top level: BBa_J176171
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215323/range2215323/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215323/range2215323/1
type: Range
persistent identity: range2215323
display id: range2215323
version: 1
direction: direction/forward
top level: BBa_J176171
start: 2288
end: 2417
orientation: inline

DD122 B
type: SequenceAnnotation
persistent identity: annotation2215309
display id: annotation2215309
version: 1
top level: BBa_J176171
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215309/range2215309/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215309/range2215309/1
type: Range
persistent identity: range2215309
display id: range2215309
version: 1
direction: direction/forward
top level: BBa_J176171
start: 186
end: 203
orientation: inline

pCMV remnant
type: SequenceAnnotation
persistent identity: annotation2215310
display id: annotation2215310
version: 1
top level: BBa_J176171
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176171/annotation2215310/range2215310/1

https://synbiohub.org/public/igem/BBa_J176171/annotation2215310/range2215310/1
type: Range
persistent identity: range2215310
display id: range2215310
version: 1
direction: direction/forward
top level: BBa_J176171
start: 232
end: 249
orientation: inline

K592200
type: ComponentDefinition
persistent identity: BBa K592200
display id: BBa_K592200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Erik Gullberg, Antonio Ascue Avalos, Erik Lundin
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 10133
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K592200 is a BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: To make the plasmid usable for Lambda Red recombineering, all sequences with homologies to the E coli chromosome had to be removed. To accomplish this, the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable provenance: Salmonella phage P22 late terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Spectinomycin resistance marker
sequence annotation: Terminator BBa_B0054
sequence annotation: Translational stop BBa_B0042
sequence annotation: Lambda red F primer
sequence annotation: VR site BBa_G00102
sequence annotation: T3 terminator BBa_B0055
sequence annotation: p15A low to medium copy replication origin BBa_I50032
sequence annotation: P22 late terminator
sequence annotation: VF2 site BBa_G00100
sequence annotation: Terminator BBa_B0062
sequence annotation: Lambda Red R primer
sequence annotation: SpcR promoter
sequence: https://synbiohub.org/public/igem/BBa_K592200_sequence/1

Spectinomycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2136375
display id: annotation2136375
version: 1
top level: K592200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136375/range2136375/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136375/range2136375/1
type: Range
persistent identity: range2136375
display id: range2136375
version: 1
direction: direction/reverse
top level: K592200
start: 1841
end: 2840
orientation: inline

Terminator BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2136370
display id: annotation2136370
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136370/range2136370/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136370/range2136370/1
type: Range
persistent identity: range2136370
display id: range2136370
version: 1
direction: direction/forward
top level: K592200
start: 47
end: 115
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2136380
display id: annotation2136380
version: 1
top level: K592200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136380/range2136380/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136380/range2136380/1
type: Range
persistent identity: range2136380
display id: range2136380
version: 1
direction: direction/forward
top level: K592200
start: 3091
end: 3102
orientation: inline

Lambda red F primer
type: SequenceAnnotation
persistent identity: annotation2136374
display id: annotation2136374
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136374/range2136374/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136374/range2136374/1
type: Range
persistent identity: range2136374
display id: range2136374
version: 1
direction: direction/forward
top level: K592200
start: 1726
end: 1745
orientation: inline

VR site BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2136371
display id: annotation2136371
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136371/range2136371/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136371/range2136371/1
type: Range
persistent identity: range2136371
display id: range2136371
version: 1
direction: direction/reverse
top level: K592200
start: 116
end: 135
orientation: inline

T3 terminator BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2136379
display id: annotation2136379
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136379/range2136379/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136379/range2136379/1
type: Range
persistent identity: range2136379
display id: range2136379
version: 1
direction: direction/forward
top level: K592200
start: 3013
end: 3090
orientation: inline

p15A low to medium copy replication origin BBa_I50032
type: SequenceAnnotation
persistent identity: annotation2136373
display id: annotation2136373
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136373/range2136373/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136373/range2136373/1
type: Range
persistent identity: range2136373
display id: range2136373
version: 1
direction: direction/forward
top level: K592200
start: 184
end: 1748
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2136377
display id: annotation2136377
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136377/range2136377/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136377/range2136377/1
type: Range
persistent identity: range2136377
display id: range2136377
version: 1
direction: direction/forward
top level: K592200
start: 2952
end: 2992
orientation: inline

VF2 site BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2136378
display id: annotation2136378
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136378/range2136378/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136378/range2136378/1
type: Range
persistent identity: range2136378
display id: range2136378
version: 1
direction: direction/forward
top level: K592200
start: 2993
end: 3012
orientation: inline

Terminator BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2136372
display id: annotation2136372
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136372/range2136372/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136372/range2136372/1
type: Range
persistent identity: range2136372
display id: range2136372
version: 1
direction: direction/forward
top level: K592200
start: 136
end: 176
orientation: inline

Lambda Red R primer
type: SequenceAnnotation
persistent identity: annotation2136398
display id: annotation2136398
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136398/range2136398/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136398/range2136398/1
type: Range
persistent identity: range2136398
display id: range2136398
version: 1
direction: direction/reverse
top level: K592200
start: 20
end: 41
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2136369
display id: annotation2136369
version: 1
top level: K592200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136369/range2136369/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136369/range2136369/1
type: Range
persistent identity: range2136369
display id: range2136369
version: 1
direction: direction/forward
top level: K592200
start: 35
end: 46
orientation: inline

SpcR promoter
type: SequenceAnnotation
persistent identity: annotation2136376
display id: annotation2136376
version: 1
top level: K592200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136376/range2136376/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136376/range2136376/1
type: Range
persistent identity: range2136376
display id: range2136376
version: 1
direction: direction/reverse
top level: K592200
start: 2907
end: 2933
orientation: inline

BBa_J119047
type: ComponentDefinition
persistent identity: BBa J119047
display id: BBa_J119047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1T8
creator: A. Malcolm Campbell and Todd Eckdahl
created: 2012-01-23T12:00:00Z
modified: 2015-08-31T04:08:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Insulated vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix.
mutable notes: Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.
mutable provenance: de novo DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Suffix
sequence annotation: VF2 primer
sequence annotation: Tetracycline resistance gene
sequence annotation: stuffer
sequence annotation: VR primer
sequence annotation: B0025 terminator
sequence annotation: c -> g BbsI site removed
sequence annotation: Prefix
sequence annotation: B0010 terminator
sequence annotation: pMB1 replication origin
sequence: https://synbiohub.org/public/igem/BBa_J119047_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2169696
display id: annotation2169696
version: 1
top level: BBa_J119047
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119047/annotation2169696/range2169696/1

https://synbiohub.org/public/igem/BBa_J119047/annotation2169696/range2169696/1
type: Range
persistent identity: range2169696
display id: range2169696
version: 1
direction: direction/forward
top level: BBa_J119047
start: 1
end: 21
orientation: inline

VF2 primer
type: SequenceAnnotation
persistent identity: annotation2169699
display id: annotation2169699
version: 1
top level: BBa_J119047
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119047/annotation2169699/range2169699/1

https://synbiohub.org/public/igem/BBa_J119047/annotation2169699/range2169699/1
type: Range
persistent identity: range2169699
display id: range2169699
version: 1
direction: direction/forward
top level: BBa_J119047
start: 2375
end: 2394
orientation: inline

Tetracycline resistance gene
type: SequenceAnnotation
persistent identity: annotation2169703
display id: annotation2169703
version: 1
top level: BBa_J119047
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J119047/annotation2169703/range2169703/1

https://synbiohub.org/public/igem/BBa_J119047/annotation2169703/range2169703/1
type: Range
persistent identity: range2169703
display id: range2169703
version: 1
direction: direction/forward
top level: BBa_J119047
start: 1178
end: 2368
orientation: inline

stuffer
type: SequenceAnnotation
persistent identity: annotation2169741
display id: annotation2169741
version: 1
top level: BBa_J119047
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119047/annotation2169741/range2169741/1

https://synbiohub.org/public/igem/BBa_J119047/annotation2169741/range2169741/1
type: Range
persistent identity: range2169741
display id: range2169741
version: 1
direction: direction/forward
top level: BBa_J119047
start: 2535
end: 2546
orientation: inline

VR primer
type: SequenceAnnotation
persistent identity: annotation2169698
display id: annotation2169698
version: 1
top level: BBa_J119047
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J119047/annotation2169698/range2169698/1

https://synbiohub.org/public/igem/BBa_J119047/annotation2169698/range2169698/1
type: Range
persistent identity: range2169698
display id: range2169698
version: 1
direction: direction/reverse
top level: BBa_J119047
start: 208
end: 227
orientation: inline

B0025 terminator
type: SequenceAnnotation
persistent identity: annotation2169700
display id: annotation2169700
version: 1
top level: BBa_J119047
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J119047/annotation2169700/range2169700/1

https://synbiohub.org/public/igem/BBa_J119047/annotation2169700/range2169700/1
type: Range
persistent identity: range2169700
display id: range2169700
version: 1
direction: direction/reverse
top level: BBa_J119047
start: 22
end: 205
orientation: inline

c -> g BbsI site removed
type: SequenceAnnotation
persistent identity: annotation2169742
display id: annotation2169742
version: 1
top level: BBa_J119047
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J119047/annotation2169742/range2169742/1

https://synbiohub.org/public/igem/BBa_J119047/annotation2169742/range2169742/1
type: Range
persistent identity: range2169742
display id: range2169742
version: 1
direction: direction/forward
top level: BBa_J119047
start: 1831
end: 1831
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2169697
display id: annotation2169697
version: 1
top level: BBa_J119047
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J119047/annotation2169697/range2169697/1

https://synbiohub.org/public/igem/BBa_J119047/annotation2169697/range2169697/1
type: Range
persistent identity: range2169697
display id: range2169697
version: 1
direction: direction/forward
top level: BBa_J119047
start: 2513
end: 2534
orientation: inline

B0010 terminator
type: SequenceAnnotation
persistent identity: annotation2169701
display id: annotation2169701
version: 1
top level: BBa_J119047
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J119047/annotation2169701/range2169701/1

https://synbiohub.org/public/igem/BBa_J119047/annotation2169701/range2169701/1
type: Range
persistent identity: range2169701
display id: range2169701
version: 1
direction: direction/forward
top level: BBa_J119047
start: 2473
end: 2512
orientation: inline

pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation2169702
display id: annotation2169702
version: 1
top level: BBa_J119047
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J119047/annotation2169702/range2169702/1

https://synbiohub.org/public/igem/BBa_J119047/annotation2169702/range2169702/1
type: Range
persistent identity: range2169702
display id: range2169702
version: 1
direction: direction/forward
top level: BBa_J119047
start: 314
end: 928
orientation: inline

BBa_J72210
type: ComponentDefinition
persistent identity: BBa J72210
display id: BBa_J72210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kanamycin resistant BAC with R6K origin, pGlpT 5' of cloning site
creator: Josh Kittleson
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:08:29Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Kanamycin resistant BAC with R6K origin, glucose driven promoter 5' of cloning site. Can be maintained at higher copy in strains expressing pir. Referred to internally as pBacRJ2g
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_J72210_sequence/1

BBa_K176007
type: ComponentDefinition
persistent identity: BBa K176007
display id: BBa_K176007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A3 with the suffix of J61002 (mRFP)
creator: Hao Jiang, Danqian Liu, Chao Li
created: 2009-06-15T11:00:00Z
modified: 2015-05-08T01:11:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: It's the same plasmid backbone of pSB1A3, but sequence between SpeI and PstI in the suffix are from plasmid backbon J61002. SpeI-RBS-mRFP-Terminator-PstI-pSB1A3-prefix
mutable notes: This plasmid backbone is generated by cutting a part in J61002 with X+P, and ligating it into pSB1A3 cutted with X+P.
mutable provenance: pSB1A3 J61002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: B0015
sequence annotation: mRFP
sequence annotation: B0034
sequence annotation: PstI
sequence annotation: t->c in pSB1A3
sequence annotation: a part of pSB1A3
sequence annotation: g->a in pSB1A3
sequence annotation: SpeI
sequence annotation: deletion of ac in pSB1A3
sequence annotation: NotI
sequence annotation: prefix
sequence annotation: a part of J61002
sequence annotation: rrnB terminator
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K176007_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2005405
display id: annotation2005405
version: 1
top level: BBa_K176007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2005405/range2005405/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2005405/range2005405/1
type: Range
persistent identity: range2005405
display id: range2005405
version: 1
direction: direction/forward
top level: BBa_K176007
start: 741
end: 869
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2005404
display id: annotation2005404
version: 1
top level: BBa_K176007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2005404/range2005404/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2005404/range2005404/1
type: Range
persistent identity: range2005404
display id: range2005404
version: 1
direction: direction/forward
top level: BBa_K176007
start: 27
end: 707
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2005401
display id: annotation2005401
version: 1
top level: BBa_K176007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2005401/range2005401/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2005401/range2005401/1
type: Range
persistent identity: range2005401
display id: range2005401
version: 1
direction: direction/forward
top level: BBa_K176007
start: 9
end: 20
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2005408
display id: annotation2005408
version: 1
top level: BBa_K176007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2005408/range2005408/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2005408/range2005408/1
type: Range
persistent identity: range2005408
display id: range2005408
version: 1
direction: direction/forward
top level: BBa_K176007
start: 885
end: 890
orientation: inline

t->c in pSB1A3
type: SequenceAnnotation
persistent identity: annotation2028472
display id: annotation2028472
version: 1
top level: BBa_K176007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2028472/range2028472/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2028472/range2028472/1
type: Range
persistent identity: range2028472
display id: range2028472
version: 1
direction: direction/forward
top level: BBa_K176007
start: 1149
end: 1149
orientation: inline

a part of pSB1A3
type: SequenceAnnotation
persistent identity: annotation2005410
display id: annotation2005410
version: 1
top level: BBa_K176007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2005410/range2005410/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2005410/range2005410/1
type: Range
persistent identity: range2005410
display id: range2005410
version: 1
direction: direction/forward
top level: BBa_K176007
start: 878
end: 3026
orientation: inline

g->a in pSB1A3
type: SequenceAnnotation
persistent identity: annotation2028473
display id: annotation2028473
version: 1
top level: BBa_K176007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2028473/range2028473/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2028473/range2028473/1
type: Range
persistent identity: range2028473
display id: range2028473
version: 1
direction: direction/forward
top level: BBa_K176007
start: 1584
end: 1584
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2005402
display id: annotation2005402
version: 1
top level: BBa_K176007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2005402/range2005402/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2005402/range2005402/1
type: Range
persistent identity: range2005402
display id: range2005402
version: 1
direction: direction/forward
top level: BBa_K176007
start: 2
end: 7
orientation: inline

deletion of ac in pSB1A3
type: SequenceAnnotation
persistent identity: annotation2028471
display id: annotation2028471
version: 1
top level: BBa_K176007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2028471/range2028471/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2028471/range2028471/1
type: Range
persistent identity: range2028471
display id: range2028471
version: 1
direction: direction/forward
top level: BBa_K176007
start: 902
end: 903
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation2005407
display id: annotation2005407
version: 1
top level: BBa_K176007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2005407/range2005407/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2005407/range2005407/1
type: Range
persistent identity: range2005407
display id: range2005407
version: 1
direction: direction/forward
top level: BBa_K176007
start: 878
end: 885
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2005411
display id: annotation2005411
version: 1
top level: BBa_K176007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2005411/range2005411/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2005411/range2005411/1
type: Range
persistent identity: range2005411
display id: range2005411
version: 1
direction: direction/forward
top level: BBa_K176007
start: 3005
end: 3026
orientation: inline

a part of J61002
type: SequenceAnnotation
persistent identity: annotation2005409
display id: annotation2005409
version: 1
top level: BBa_K176007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2005409/range2005409/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2005409/range2005409/1
type: Range
persistent identity: range2005409
display id: range2005409
version: 1
direction: direction/forward
top level: BBa_K176007
start: 1
end: 890
orientation: inline

rrnB terminator
type: SequenceAnnotation
persistent identity: annotation2005406
display id: annotation2005406
version: 1
top level: BBa_K176007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2005406/range2005406/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2005406/range2005406/1
type: Range
persistent identity: range2005406
display id: range2005406
version: 1
direction: direction/forward
top level: BBa_K176007
start: 752
end: 795
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2005403
display id: annotation2005403
version: 1
top level: BBa_K176007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K176007/annotation2005403/range2005403/1

https://synbiohub.org/public/igem/BBa_K176007/annotation2005403/range2005403/1
type: Range
persistent identity: range2005403
display id: range2005403
version: 1
direction: direction/forward
top level: BBa_K176007
start: 9
end: 20
orientation: inline

BBa_K879918
type: ComponentDefinition
persistent identity: BBa K879918
display id: BBa_K879918
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetO ori
creator: Nien-Tsu Tseng
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: TetO ori
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: -35
sequence annotation: BioBrick suffix
sequence annotation: TetO operator
sequence annotation: BioBrick prefix
sequence annotation: RNA II
sequence annotation: pMB1 derived replication origin
sequence annotation: E coli his operon terminator
sequence annotation: Chloramphenicol resistance
sequence annotation: -10
sequence annotation: T0 terminator
sequence annotation: VF2
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_K879918_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2210756
display id: annotation2210756
version: 1
top level: BBa_K879918
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210756/range2210756/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210756/range2210756/1
type: Range
persistent identity: range2210756
display id: range2210756
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 859
end: 864
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2210749
display id: annotation2210749
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210749/range2210749/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210749/range2210749/1
type: Range
persistent identity: range2210749
display id: range2210749
version: 1
direction: direction/forward
top level: BBa_K879918
start: 1
end: 21
orientation: inline

TetO operator
type: SequenceAnnotation
persistent identity: annotation2210755
display id: annotation2210755
version: 1
top level: BBa_K879918
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210755/range2210755/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210755/range2210755/1
type: Range
persistent identity: range2210755
display id: range2210755
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 842
end: 858
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2210775
display id: annotation2210775
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210775/range2210775/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210775/range2210775/1
type: Range
persistent identity: range2210775
display id: range2210775
version: 1
direction: direction/forward
top level: BBa_K879918
start: 2053
end: 2072
orientation: inline

RNA II
type: SequenceAnnotation
persistent identity: annotation2210753
display id: annotation2210753
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210753/range2210753/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210753/range2210753/1
type: Range
persistent identity: range2210753
display id: range2210753
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 277
end: 826
orientation: inline

pMB1 derived replication origin
type: SequenceAnnotation
persistent identity: annotation2210752
display id: annotation2210752
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210752/range2210752/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210752/range2210752/1
type: Range
persistent identity: range2210752
display id: range2210752
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 263
end: 877
orientation: inline

E coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2210750
display id: annotation2210750
version: 1
top level: BBa_K879918
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210750/range2210750/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210750/range2210750/1
type: Range
persistent identity: range2210750
display id: range2210750
version: 1
direction: direction/forward
top level: BBa_K879918
start: 22
end: 95
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2210758
display id: annotation2210758
version: 1
top level: BBa_K879918
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210758/range2210758/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210758/range2210758/1
type: Range
persistent identity: range2210758
display id: range2210758
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 1162
end: 1821
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2210754
display id: annotation2210754
version: 1
top level: BBa_K879918
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210754/range2210754/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210754/range2210754/1
type: Range
persistent identity: range2210754
display id: range2210754
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 836
end: 841
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2210757
display id: annotation2210757
version: 1
top level: BBa_K879918
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210757/range2210757/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210757/range2210757/1
type: Range
persistent identity: range2210757
display id: range2210757
version: 1
direction: direction/forward
top level: BBa_K879918
start: 1044
end: 1149
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2210759
display id: annotation2210759
version: 1
top level: BBa_K879918
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210759/range2210759/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210759/range2210759/1
type: Range
persistent identity: range2210759
display id: range2210759
version: 1
direction: direction/forward
top level: BBa_K879918
start: 1935
end: 1954
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2210751
display id: annotation2210751
version: 1
top level: BBa_K879918
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210751/range2210751/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210751/range2210751/1
type: Range
persistent identity: range2210751
display id: range2210751
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 159
end: 178
orientation: inline

BBa_K1256002
type: ComponentDefinition
persistent identity: BBa K1256002
display id: BBa_K1256002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3-Plac-NB-Cm-MN
creator: Pei-Hong Chen
created: 2014-06-08T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
group u list: _1614_
m user id: 0
owner id: 8995
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: TEST
mutable notes: TEST
mutable provenance: TEST
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_E1010
sequence annotation: Chloramphenicol resistance cassette
sequence annotation: ori
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1256002_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2378948
display id: annotation2378948
version: 1
top level: BBa_K1256002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1256002/annotation2378948/range2378948/1

https://synbiohub.org/public/igem/BBa_K1256002/annotation2378948/range2378948/1
type: Range
persistent identity: range2378948
display id: range2378948
version: 1
direction: direction/forward
top level: BBa_K1256002
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2378947
display id: annotation2378947
version: 1
top level: BBa_K1256002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1256002/annotation2378947/range2378947/1

https://synbiohub.org/public/igem/BBa_K1256002/annotation2378947/range2378947/1
type: Range
persistent identity: range2378947
display id: range2378947
version: 1
direction: direction/forward
top level: BBa_K1256002
start: 1
end: 200
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2378957
display id: annotation2378957
version: 1
top level: BBa_K1256002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1256002/annotation2378957/range2378957/1

https://synbiohub.org/public/igem/BBa_K1256002/annotation2378957/range2378957/1
type: Range
persistent identity: range2378957
display id: range2378957
version: 1
direction: direction/forward
top level: BBa_K1256002
start: 227
end: 932
orientation: inline

Chloramphenicol resistance cassette
type: SequenceAnnotation
persistent identity: annotation2378960
display id: annotation2378960
version: 1
top level: BBa_K1256002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1256002/annotation2378960/range2378960/1

https://synbiohub.org/public/igem/BBa_K1256002/annotation2378960/range2378960/1
type: Range
persistent identity: range2378960
display id: range2378960
version: 1
direction: direction/reverse
top level: BBa_K1256002
start: 2256
end: 2915
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2378959
display id: annotation2378959
version: 1
top level: BBa_K1256002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1256002/annotation2378959/range2378959/1

https://synbiohub.org/public/igem/BBa_K1256002/annotation2378959/range2378959/1
type: Range
persistent identity: range2378959
display id: range2378959
version: 1
direction: direction/forward
top level: BBa_K1256002
start: 1335
end: 1948
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2378958
display id: annotation2378958
version: 1
top level: BBa_K1256002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1256002/annotation2378958/range2378958/1

https://synbiohub.org/public/igem/BBa_K1256002/annotation2378958/range2378958/1
type: Range
persistent identity: range2378958
display id: range2378958
version: 1
direction: direction/forward
top level: BBa_K1256002
start: 947
end: 1075
orientation: inline

pMA-BBFR
type: ComponentDefinition
persistent identity: BBa K157000
display id: BBa_K157000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMA-BBFR - plasmid description
creator: Raik Gruenberg and iGEM Team Freiburg
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _232_
m user id: 0
owner id: 1675
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid with BioBrick 3.0 prefix and suffix
mutable notes: none
mutable provenance: Plasmid designed by Raik Groenberg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: AmpR
sequence annotation: AgeI
sequence annotation: Suffix BioBrick 3.0
sequence annotation: NgoMIV
sequence annotation: Prefix BioBrick 3.0
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K157000_sequence/1

AmpR
type: SequenceAnnotation
persistent identity: annotation1986394
display id: annotation1986394
version: 1
top level: pMA-BBFR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986394/range1986394/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986394/range1986394/1
type: Range
persistent identity: range1986394
display id: range1986394
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 541
end: 1398
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1986391
display id: annotation1986391
version: 1
top level: pMA-BBFR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986391/range1986391/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986391/range1986391/1
type: Range
persistent identity: range1986391
display id: range1986391
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 1
end: 6
orientation: inline

Suffix BioBrick 3.0
type: SequenceAnnotation
persistent identity: annotation1986392
display id: annotation1986392
version: 1
top level: pMA-BBFR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986392/range1986392/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986392/range1986392/1
type: Range
persistent identity: range1986392
display id: range1986392
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 1
end: 30
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2064660
display id: annotation2064660
version: 1
top level: pMA-BBFR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157000/annotation2064660/range2064660/1

https://synbiohub.org/public/igem/BBa_K157000/annotation2064660/range2064660/1
type: Range
persistent identity: range2064660
display id: range2064660
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 2423
end: 2428
orientation: inline

Prefix BioBrick 3.0
type: SequenceAnnotation
persistent identity: annotation1986395
display id: annotation1986395
version: 1
top level: pMA-BBFR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986395/range1986395/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986395/range1986395/1
type: Range
persistent identity: range1986395
display id: range1986395
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 2400
end: 2428
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1986393
display id: annotation1986393
version: 1
top level: pMA-BBFR
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986393/range1986393/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986393/range1986393/1
type: Range
persistent identity: range1986393
display id: range1986393
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 7
end: 9
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1986396
display id: annotation1986396
version: 1
top level: pMA-BBFR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986396/range1986396/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986396/range1986396/1
type: Range
persistent identity: range1986396
display id: range1986396
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 2420
end: 2422
orientation: inline

BBa_K1364021
type: ComponentDefinition
persistent identity: BBa K1364021
display id: BBa_K1364021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Integrative plasmid for Bacillus subtilis (pSBbs4E)
creator: Jourdan Camille
created: 2014-10-02T11:00:00Z
modified: 2015-06-08T09:20:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1740_
m user id: 4206
owner id: 21465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: bla
mutable notes: bla
mutable provenance: bla
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ermB
sequence annotation: 'thrC
sequence annotation: thrC'
sequence annotation: amp
sequence: https://synbiohub.org/public/igem/BBa_K1364021_sequence/1

ermB
type: SequenceAnnotation
persistent identity: annotation2425222
display id: annotation2425222
version: 1
top level: BBa_K1364021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1364021/annotation2425222/range2425222/1

https://synbiohub.org/public/igem/BBa_K1364021/annotation2425222/range2425222/1
type: Range
persistent identity: range2425222
display id: range2425222
version: 1
direction: direction/forward
top level: BBa_K1364021
start: 288
end: 1047
orientation: inline

'thrC
type: SequenceAnnotation
persistent identity: annotation2425223
display id: annotation2425223
version: 1
top level: BBa_K1364021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1364021/annotation2425223/range2425223/1

https://synbiohub.org/public/igem/BBa_K1364021/annotation2425223/range2425223/1
type: Range
persistent identity: range2425223
display id: range2425223
version: 1
direction: direction/forward
top level: BBa_K1364021
start: 1181
end: 1736
orientation: inline

thrC'
type: SequenceAnnotation
persistent identity: annotation2425225
display id: annotation2425225
version: 1
top level: BBa_K1364021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1364021/annotation2425225/range2425225/1

https://synbiohub.org/public/igem/BBa_K1364021/annotation2425225/range2425225/1
type: Range
persistent identity: range2425225
display id: range2425225
version: 1
direction: direction/forward
top level: BBa_K1364021
start: 4661
end: 5163
orientation: inline

amp
type: SequenceAnnotation
persistent identity: annotation2425224
display id: annotation2425224
version: 1
top level: BBa_K1364021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1364021/annotation2425224/range2425224/1

https://synbiohub.org/public/igem/BBa_K1364021/annotation2425224/range2425224/1
type: Range
persistent identity: range2425224
display id: range2425224
version: 1
direction: direction/forward
top level: BBa_K1364021
start: 3347
end: 4205
orientation: inline

BBa_K906201
type: ComponentDefinition
persistent identity: BBa K906201
display id: BBa_K906201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Psl2131 (for Synechocystis homologous recombination)
creator: Bert Berla
created: 2012-08-13T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1171_
m user id: 0
owner id: 12629
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Kan/Amp resistant plasmid for Homogous recombination into Synechocystis 6803. Please note that this plasmid is only biobrick friendly and not compatible due to an EcoRI site present above the upstream homology region. To use this with biobrick compatible parts, assembly should be completed in a standard, highcopy plasmid such as Psb1C3 and then inserted into this backbone for transformation into Synechocystis using XbaI and PstI.
mutable notes: This plasmid has both Kan and Amp resistance.
mutable provenance: This plasmid backbone was made by Bert Berla. It is a pUC19 derivitive.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

BBa_K1137003
type: ComponentDefinition
persistent identity: BBa K1137003
display id: BBa_K1137003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB9S1
creator: Matthew Deyell
created: 2013-10-03T11:00:00Z
modified: 2015-05-08T01:09:21Z
discontinued: false
dominant: false
group u list: _1449_
m user id: 0
owner id: 15098
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Contains the mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with CloDF13 replicon with a copy number of 20 ??? 40 per cell. The cat gene has been replaced with aadA conveying spectinomycin and streptomycin resistance.
mutable notes: The terminators in the pSB1C3 made gibson assembly difficult and as such we switched to traditional cloning using XhoI and AvrII cut sites.
mutable provenance: Upgraded pSB1C3 swapping out the origin and resistance cassettes for CloDF13 and aadA from pCDF
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: terminator
sequence annotation: CloDF13 ori
sequence annotation: prefix
sequence annotation: mRFP
sequence annotation: aadA
sequence annotation: suffix
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_K1137003_sequence/1

terminator
type: SequenceAnnotation
persistent identity: annotation2367777
display id: annotation2367777
version: 1
top level: BBa_K1137003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137003/annotation2367777/range2367777/1

https://synbiohub.org/public/igem/BBa_K1137003/annotation2367777/range2367777/1
type: Range
persistent identity: range2367777
display id: range2367777
version: 1
direction: direction/forward
top level: BBa_K1137003
start: 1887
end: 1927
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2367775
display id: annotation2367775
version: 1
top level: BBa_K1137003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137003/annotation2367775/range2367775/1

https://synbiohub.org/public/igem/BBa_K1137003/annotation2367775/range2367775/1
type: Range
persistent identity: range2367775
display id: range2367775
version: 1
direction: direction/reverse
top level: BBa_K1137003
start: 1705
end: 1726
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2367776
display id: annotation2367776
version: 1
top level: BBa_K1137003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137003/annotation2367776/range2367776/1

https://synbiohub.org/public/igem/BBa_K1137003/annotation2367776/range2367776/1
type: Range
persistent identity: range2367776
display id: range2367776
version: 1
direction: direction/reverse
top level: BBa_K1137003
start: 1762
end: 1784
orientation: inline

CloDF13 ori
type: SequenceAnnotation
persistent identity: annotation2367774
display id: annotation2367774
version: 1
top level: BBa_K1137003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1137003/annotation2367774/range2367774/1

https://synbiohub.org/public/igem/BBa_K1137003/annotation2367774/range2367774/1
type: Range
persistent identity: range2367774
display id: range2367774
version: 1
direction: direction/reverse
top level: BBa_K1137003
start: 1050
end: 1788
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2367781
display id: annotation2367781
version: 1
top level: BBa_K1137003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1137003/annotation2367781/range2367781/1

https://synbiohub.org/public/igem/BBa_K1137003/annotation2367781/range2367781/1
type: Range
persistent identity: range2367781
display id: range2367781
version: 1
direction: direction/forward
top level: BBa_K1137003
start: 1929
end: 1949
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2367779
display id: annotation2367779
version: 1
top level: BBa_K1137003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1137003/annotation2367779/range2367779/1

https://synbiohub.org/public/igem/BBa_K1137003/annotation2367779/range2367779/1
type: Range
persistent identity: range2367779
display id: range2367779
version: 1
direction: direction/forward
top level: BBa_K1137003
start: 2177
end: 2854
orientation: inline

aadA
type: SequenceAnnotation
persistent identity: annotation2367771
display id: annotation2367771
version: 1
top level: BBa_K1137003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1137003/annotation2367771/range2367771/1

https://synbiohub.org/public/igem/BBa_K1137003/annotation2367771/range2367771/1
type: Range
persistent identity: range2367771
display id: range2367771
version: 1
direction: direction/reverse
top level: BBa_K1137003
start: 122
end: 910
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2367785
display id: annotation2367785
version: 1
top level: BBa_K1137003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1137003/annotation2367785/range2367785/1

https://synbiohub.org/public/igem/BBa_K1137003/annotation2367785/range2367785/1
type: Range
persistent identity: range2367785
display id: range2367785
version: 1
direction: direction/forward
top level: BBa_K1137003
start: 3021
end: 3040
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2367784
display id: annotation2367784
version: 1
top level: BBa_K1137003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137003/annotation2367784/range2367784/1

https://synbiohub.org/public/igem/BBa_K1137003/annotation2367784/range2367784/1
type: Range
persistent identity: range2367784
display id: range2367784
version: 1
direction: direction/forward
top level: BBa_K1137003
start: 3041
end: 3110
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation2367783
display id: annotation2367783
version: 1
top level: BBa_K1137003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1137003/annotation2367783/range2367783/1

https://synbiohub.org/public/igem/BBa_K1137003/annotation2367783/range2367783/1
type: Range
persistent identity: range2367783
display id: range2367783
version: 1
direction: direction/forward
top level: BBa_K1137003
start: 2986
end: 3005
orientation: inline

BBa_K1945011
type: ComponentDefinition
persistent identity: BBa K1945011
display id: BBa_K1945011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3 HR Plasmid
creator: Sharon Lian
created: 2016-10-20T11:00:00Z
modified: 2016-10-28T04:49:52Z
discontinued: false
dominant: false
group u list: _2412_
m user id: 27623
owner id: 27623
owning group id: 9
status: status/Planning
bookmark: false
mutable description: WIP
mutable notes: WIP
mutable provenance: WIP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1945011_sequence/1

BBa_J140000
type: ComponentDefinition
persistent identity: BBa J140000
display id: BBa_J140000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Vaccinia virus integration backbone
creator: Darren Nesbeth
created: 2015-06-19T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: false
group u list: _641_
m user id: 0
owner id: 4717
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: Pending
mutable notes: Absence of illegal sites in Vaccinia virus TK gene
mutable provenance: Pending
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone

BBa_K1473005
type: ComponentDefinition
persistent identity: BBa K1473005
display id: BBa_K1473005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phagemid pBluescript
creator: Shan, Zhe
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1852_
m user id: 0
owner id: 20914
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a phagemid. It functions as a normal plasmid when transformed into bacteria. But when helper phage M13KO7 infect this bacteria, it will produce progeny M13 phage. Phagemid pBluescript contains a f1 ori while M13KO7 helper phage's package signal is modified as knock-down. So after the infection of M13KO7, its genome can produce protein but these proteins will see pBluescript as their true ???genome???. As a result, the progeny phage???s genome will only contains pBluescript, so they cannot produce next generation by infection. We have removed some sequence on its MCS to create a linearized form of this phagemid.
mutable notes: Normally, a pBluescript contains EcoRI, PstI, XbaI and SpeI on its MCS. We have removed these sequence to create a linearized form of this phagemid.
mutable provenance: Its f1 ori comes from phage M13.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: f1 ori
sequence annotation: AmpR promoter
sequence annotation: ori
sequence annotation: AmpR
sequence: https://synbiohub.org/public/igem/BBa_K1473005_sequence/1

f1 ori
type: SequenceAnnotation
persistent identity: annotation2383839
display id: annotation2383839
version: 1
top level: BBa_K1473005
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1473005/annotation2383839/range2383839/1

https://synbiohub.org/public/igem/BBa_K1473005/annotation2383839/range2383839/1
type: Range
persistent identity: range2383839
display id: range2383839
version: 1
direction: direction/forward
top level: BBa_K1473005
start: 2211
end: 2666
orientation: inline

AmpR promoter
type: SequenceAnnotation
persistent identity: annotation2383841
display id: annotation2383841
version: 1
top level: BBa_K1473005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1473005/annotation2383841/range2383841/1

https://synbiohub.org/public/igem/BBa_K1473005/annotation2383841/range2383841/1
type: Range
persistent identity: range2383841
display id: range2383841
version: 1
direction: direction/reverse
top level: BBa_K1473005
start: 2080
end: 2185
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2383838
display id: annotation2383838
version: 1
top level: BBa_K1473005
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1473005/annotation2383838/range2383838/1

https://synbiohub.org/public/igem/BBa_K1473005/annotation2383838/range2383838/1
type: Range
persistent identity: range2383838
display id: range2383838
version: 1
direction: direction/reverse
top level: BBa_K1473005
start: 460
end: 1048
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2383840
display id: annotation2383840
version: 1
top level: BBa_K1473005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1473005/annotation2383840/range2383840/1

https://synbiohub.org/public/igem/BBa_K1473005/annotation2383840/range2383840/1
type: Range
persistent identity: range2383840
display id: range2383840
version: 1
direction: direction/reverse
top level: BBa_K1473005
start: 1219
end: 2079
orientation: inline

BBa_K408001
type: ComponentDefinition
persistent identity: BBa K408001
display id: BBa_K408001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBlueScript
creator: Pavan Choppakatla
created: 2010-11-05T12:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
group u list: _523_
m user id: 0
owner id: 6477
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Uses this typical vector as a substitute for Biobrick vectors to enable easier selection by blue white screening.
mutable notes: Obtained directly from commercial sources and used specifically to enable insertion of BBa_K408000.
mutable provenance: Typical commercial vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K408001_sequence/1

BBa_K1321308
type: ComponentDefinition
persistent identity: BBa K1321308
display id: BBa_K1321308
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBla-Vhb-122-Bb
creator: Michael Florea
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20779
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: X
mutable notes: Z
mutable provenance: Y
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Biobrick prefix
sequence annotation: Replication protein
sequence annotation: Biobrick suffix
sequence annotation: Mobilization protein (contains a 4bp deletion)
sequence annotation: KanR
sequence: https://synbiohub.org/public/igem/BBa_K1321308_sequence/1

Biobrick prefix
type: SequenceAnnotation
persistent identity: annotation2425411
display id: annotation2425411
version: 1
top level: BBa_K1321308
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321308/annotation2425411/range2425411/1

https://synbiohub.org/public/igem/BBa_K1321308/annotation2425411/range2425411/1
type: Range
persistent identity: range2425411
display id: range2425411
version: 1
direction: direction/forward
top level: BBa_K1321308
start: 4667
end: 4688
orientation: inline

Replication protein
type: SequenceAnnotation
persistent identity: annotation2425408
display id: annotation2425408
version: 1
top level: BBa_K1321308
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321308/annotation2425408/range2425408/1

https://synbiohub.org/public/igem/BBa_K1321308/annotation2425408/range2425408/1
type: Range
persistent identity: range2425408
display id: range2425408
version: 1
direction: direction/reverse
top level: BBa_K1321308
start: 480
end: 1154
orientation: inline

Biobrick suffix
type: SequenceAnnotation
persistent identity: annotation2425407
display id: annotation2425407
version: 1
top level: BBa_K1321308
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321308/annotation2425407/range2425407/1

https://synbiohub.org/public/igem/BBa_K1321308/annotation2425407/range2425407/1
type: Range
persistent identity: range2425407
display id: range2425407
version: 1
direction: direction/forward
top level: BBa_K1321308
start: 1
end: 21
orientation: inline

Mobilization protein (contains a 4bp deletion)
type: SequenceAnnotation
persistent identity: annotation2425410
display id: annotation2425410
version: 1
top level: BBa_K1321308
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321308/annotation2425410/range2425410/1

https://synbiohub.org/public/igem/BBa_K1321308/annotation2425410/range2425410/1
type: Range
persistent identity: range2425410
display id: range2425410
version: 1
direction: direction/forward
top level: BBa_K1321308
start: 3393
end: 4416
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2425409
display id: annotation2425409
version: 1
top level: BBa_K1321308
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1321308/annotation2425409/range2425409/1

https://synbiohub.org/public/igem/BBa_K1321308/annotation2425409/range2425409/1
type: Range
persistent identity: range2425409
display id: range2425409
version: 1
direction: direction/forward
top level: BBa_K1321308
start: 1680
end: 2495
orientation: inline

pSB2K3
type: ComponentDefinition
persistent identity: pSB2K3
display id: pSB2K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible copy number BioBrick plasmid
creator: Sri Kosuri
created: 2004-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_48_46_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB2K3 is a variable copy number plasmid carrying kanamycin resistance. The plasmid contains the F' replication origin and also the P1 lytic replication origin. Genes encoding proteins that are required to activate the P1 lytic replication origin are carried on the plasmid under the control of a lacI-regulated promoter. When grown in cells producing LacI the plasmid is maintained at very low copy (<10 per cell). When induced with IPTG the P1 lytic replication proteins are produced and the P1 lytic replication origin takes over, raising the copy number to very high levels (>100++ per cell). The copy control features of pSB2K3 are useful for cloning "difficult" parts while still being able to prepare large amounts of DNA. Additionally, pSB2K3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region.
mutable notes: *** pSCANS via BNL *** The pSB2 series plasmids are based on the pSCANS-5 plasmid from John Dunn's group at Brookhaven National Laboratory. The pSCANS plasmids are introduced here.
mutable provenance: MIT SBWG modification of pSCANS-5 from BNL
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: E. coli his operon terminator
sequence annotation: kanR
sequence annotation: repL
sequence annotation: repE (rev)
sequence annotation: terminator stem loop
sequence annotation: suffix
sequence annotation: VR
sequence annotation: lacOP
sequence annotation: end of terminator
sequence annotation: nnpp promoters
sequence annotation: deletion of two bases
sequence annotation: F1 Replication Origin
sequence annotation: VF2
sequence annotation: prefix
sequence annotation: oriS Replication Origin (rev)
sequence: https://synbiohub.org/public/igem/pSB2K3_sequence/1

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824015
display id: annotation1824015
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824015/range1824015/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824015/range1824015/1
type: Range
persistent identity: range1824015
display id: range1824015
version: 1
direction: direction/forward
top level: pSB2K3
start: 22
end: 93
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation1023020
display id: annotation1023020
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023020/range1023020/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023020/range1023020/1
type: Range
persistent identity: range1023020
display id: range1023020
version: 1
direction: direction/forward
top level: pSB2K3
start: 3433
end: 4248
orientation: inline

repL
type: SequenceAnnotation
persistent identity: annotation1023027
display id: annotation1023027
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023027/range1023027/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023027/range1023027/1
type: Range
persistent identity: range1023027
display id: range1023027
version: 1
direction: direction/forward
top level: pSB2K3
start: 2289
end: 3134
orientation: inline

repE (rev)
type: SequenceAnnotation
persistent identity: annotation1023024
display id: annotation1023024
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023024/range1023024/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023024/range1023024/1
type: Range
persistent identity: range1023024
display id: range1023024
version: 1
direction: direction/reverse
top level: pSB2K3
start: 617
end: 1372
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824014
display id: annotation1824014
version: 1
top level: pSB2K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824014/range1824014/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824014/range1824014/1
type: Range
persistent identity: range1824014
display id: range1824014
version: 1
direction: direction/forward
top level: pSB2K3
start: 30
end: 64
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1023022
display id: annotation1023022
version: 1
top level: pSB2K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023022/range1023022/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023022/range1023022/1
type: Range
persistent identity: range1023022
display id: range1023022
version: 1
direction: direction/forward
top level: pSB2K3
start: 1
end: 21
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782483
display id: annotation1782483
version: 1
top level: pSB2K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K3/annotation1782483/range1782483/1

https://synbiohub.org/public/igem/pSB2K3/annotation1782483/range1782483/1
type: Range
persistent identity: range1782483
display id: range1782483
version: 1
direction: direction/reverse
top level: pSB2K3
start: 157
end: 176
orientation: inline

lacOP
type: SequenceAnnotation
persistent identity: annotation1023026
display id: annotation1023026
version: 1
top level: pSB2K3
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023026/range1023026/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023026/range1023026/1
type: Range
persistent identity: range1023026
display id: range1023026
version: 1
direction: direction/forward
top level: pSB2K3
start: 2037
end: 2211
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824017
display id: annotation1824017
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824017/range1824017/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824017/range1824017/1
type: Range
persistent identity: range1824017
display id: range1824017
version: 1
direction: direction/reverse
top level: pSB2K3
start: 4362
end: 4365
orientation: inline

nnpp promoters
type: SequenceAnnotation
persistent identity: annotation1023028
display id: annotation1023028
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023028/range1023028/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023028/range1023028/1
type: Range
persistent identity: range1023028
display id: range1023028
version: 1
direction: direction/forward
top level: pSB2K3
start: 3242
end: 3393
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823781
display id: annotation1823781
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1823781/range1823781/1

https://synbiohub.org/public/igem/pSB2K3/annotation1823781/range1823781/1
type: Range
persistent identity: range1823781
display id: range1823781
version: 1
direction: direction/forward
top level: pSB2K3
start: 34
end: 35
orientation: inline

F1 Replication Origin
type: SequenceAnnotation
persistent identity: annotation1023023
display id: annotation1023023
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023023/range1023023/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023023/range1023023/1
type: Range
persistent identity: range1023023
display id: range1023023
version: 1
direction: direction/forward
top level: pSB2K3
start: 242
end: 290
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824016
display id: annotation1824016
version: 1
top level: pSB2K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824016/range1824016/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824016/range1824016/1
type: Range
persistent identity: range1824016
display id: range1824016
version: 1
direction: direction/reverse
top level: pSB2K3
start: 4375
end: 4394
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782484
display id: annotation1782484
version: 1
top level: pSB2K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K3/annotation1782484/range1782484/1

https://synbiohub.org/public/igem/pSB2K3/annotation1782484/range1782484/1
type: Range
persistent identity: range1782484
display id: range1782484
version: 1
direction: direction/forward
top level: pSB2K3
start: 4286
end: 4305
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1023021
display id: annotation1023021
version: 1
top level: pSB2K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023021/range1023021/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023021/range1023021/1
type: Range
persistent identity: range1023021
display id: range1023021
version: 1
direction: direction/forward
top level: pSB2K3
start: 4404
end: 4425
orientation: inline

oriS Replication Origin (rev)
type: SequenceAnnotation
persistent identity: annotation1023025
display id: annotation1023025
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023025/range1023025/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023025/range1023025/1
type: Range
persistent identity: range1023025
display id: range1023025
version: 1
direction: direction/reverse
top level: pSB2K3
start: 1463
end: 1679
orientation: inline

BBa_J176122
type: ComponentDefinition
persistent identity: BBa J176122
display id: BBa_J176122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcDNA3.1+ puro
creator: Brady Laughlin
created: 2011-10-20T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pcDNA3.1+ puro/ ΔpCMV, based on pcDNA3.1+; no promoter; amp, puro
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Puromycin resistance
sequence annotation: SV40
sequence annotation: Ampicillin resistance
sequence annotation: STOP in frame w/ XbaI
sequence annotation: DD122B
sequence annotation: bla
sequence annotation: BGH
sequence annotation: f1 origin
sequence annotation: pCMV remnant
sequence annotation: SpeI EcoRI PstI NotI XbaI
sequence annotation: DD123B
sequence: https://synbiohub.org/public/igem/BBa_J176122_sequence/1

Puromycin resistance
type: SequenceAnnotation
persistent identity: annotation2167163
display id: annotation2167163
version: 1
top level: BBa_J176122
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167163/range2167163/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167163/range2167163/1
type: Range
persistent identity: range2167163
display id: range2167163
version: 1
direction: direction/forward
top level: BBa_J176122
start: 1393
end: 1992
orientation: inline

SV40
type: SequenceAnnotation
persistent identity: annotation2167162
display id: annotation2167162
version: 1
top level: BBa_J176122
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167162/range2167162/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167162/range2167162/1
type: Range
persistent identity: range2167162
display id: range2167162
version: 1
direction: direction/forward
top level: BBa_J176122
start: 1045
end: 1389
orientation: inline

Ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation2167165
display id: annotation2167165
version: 1
top level: BBa_J176122
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167165/range2167165/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167165/range2167165/1
type: Range
persistent identity: range2167165
display id: range2167165
version: 1
direction: direction/reverse
top level: BBa_J176122
start: 3533
end: 4393
orientation: inline

STOP in frame w/ XbaI
type: SequenceAnnotation
persistent identity: annotation2167157
display id: annotation2167157
version: 1
top level: BBa_J176122
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167157/range2167157/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167157/range2167157/1
type: Range
persistent identity: range2167157
display id: range2167157
version: 1
direction: direction/forward
top level: BBa_J176122
start: 329
end: 331
orientation: inline

DD122B
type: SequenceAnnotation
persistent identity: annotation2167153
display id: annotation2167153
version: 1
top level: BBa_J176122
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167153/range2167153/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167153/range2167153/1
type: Range
persistent identity: range2167153
display id: range2167153
version: 1
direction: direction/forward
top level: BBa_J176122
start: 186
end: 203
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2167177
display id: annotation2167177
version: 1
top level: BBa_J176122
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167177/range2167177/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167177/range2167177/1
type: Range
persistent identity: range2167177
display id: range2167177
version: 1
direction: direction/reverse
top level: BBa_J176122
start: 4394
end: 4492
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2167158
display id: annotation2167158
version: 1
top level: BBa_J176122
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167158/range2167158/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167158/range2167158/1
type: Range
persistent identity: range2167158
display id: range2167158
version: 1
direction: direction/reverse
top level: BBa_J176122
start: 336
end: 353
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2167161
display id: annotation2167161
version: 1
top level: BBa_J176122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167161/range2167161/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167161/range2167161/1
type: Range
persistent identity: range2167161
display id: range2167161
version: 1
direction: direction/forward
top level: BBa_J176122
start: 612
end: 1040
orientation: inline

pCMV remnant
type: SequenceAnnotation
persistent identity: annotation2167154
display id: annotation2167154
version: 1
top level: BBa_J176122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167154/range2167154/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167154/range2167154/1
type: Range
persistent identity: range2167154
display id: range2167154
version: 1
direction: direction/forward
top level: BBa_J176122
start: 232
end: 249
orientation: inline

BGH
type: SequenceAnnotation
persistent identity: annotation2167159
display id: annotation2167159
version: 1
top level: BBa_J176122
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167159/range2167159/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167159/range2167159/1
type: Range
persistent identity: range2167159
display id: range2167159
version: 1
direction: direction/forward
top level: BBa_J176122
start: 342
end: 566
orientation: inline

SpeI EcoRI PstI NotI XbaI
type: SequenceAnnotation
persistent identity: annotation2167155
display id: annotation2167155
version: 1
top level: BBa_J176122
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167155/range2167155/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167155/range2167155/1
type: Range
persistent identity: range2167155
display id: range2167155
version: 1
direction: direction/forward
top level: BBa_J176122
start: 250
end: 324
orientation: inline

DD123B
type: SequenceAnnotation
persistent identity: annotation2167160
display id: annotation2167160
version: 1
top level: BBa_J176122
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167160/range2167160/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167160/range2167160/1
type: Range
persistent identity: range2167160
display id: range2167160
version: 1
direction: direction/reverse
top level: BBa_J176122
start: 344
end: 362
orientation: inline

STOP in frame w/ XbaI
type: SequenceAnnotation
persistent identity: annotation2167156
display id: annotation2167156
version: 1
top level: BBa_J176122
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167156/range2167156/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167156/range2167156/1
type: Range
persistent identity: range2167156
display id: range2167156
version: 1
direction: direction/forward
top level: BBa_J176122
start: 320
end: 322
orientation: inline

SV40
type: SequenceAnnotation
persistent identity: annotation2167164
display id: annotation2167164
version: 1
top level: BBa_J176122
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_J176122/annotation2167164/range2167164/1

https://synbiohub.org/public/igem/BBa_J176122/annotation2167164/range2167164/1
type: Range
persistent identity: range2167164
display id: range2167164
version: 1
direction: direction/forward
top level: BBa_J176122
start: 2206
end: 2335
orientation: inline

Transfecti
type: ComponentDefinition
persistent identity: BBa K157041
display id: BBa_K157041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transfection vector (similar to pSB1AK8)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick prefix (missing a 3' G)
sequence annotation: VF2
sequence annotation: kanamycin resistance marker
sequence annotation: Eukaryotic terminator
sequence annotation: rep (pMB1)
sequence annotation: BioBrick suffix (missing a C)
sequence annotation: ampicillin resistance marker
sequence annotation: Stem loop
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_K157041_sequence/1

BioBrick prefix (missing a 3' G)
type: SequenceAnnotation
persistent identity: annotation2064659
display id: annotation2064659
version: 1
top level: Transfecti
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157041/annotation2064659/range2064659/1

https://synbiohub.org/public/igem/BBa_K157041/annotation2064659/range2064659/1
type: Range
persistent identity: range2064659
display id: range2064659
version: 1
direction: direction/forward
top level: Transfecti
start: 3405
end: 3425
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2064658
display id: annotation2064658
version: 1
top level: Transfecti
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K157041/annotation2064658/range2064658/1

https://synbiohub.org/public/igem/BBa_K157041/annotation2064658/range2064658/1
type: Range
persistent identity: range2064658
display id: range2064658
version: 1
direction: direction/forward
top level: Transfecti
start: 3207
end: 3226
orientation: inline

kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2064656
display id: annotation2064656
version: 1
top level: Transfecti
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157041/annotation2064656/range2064656/1

https://synbiohub.org/public/igem/BBa_K157041/annotation2064656/range2064656/1
type: Range
persistent identity: range2064656
display id: range2064656
version: 1
direction: direction/reverse
top level: Transfecti
start: 1334
end: 2149
orientation: inline

Eukaryotic terminator
type: SequenceAnnotation
persistent identity: annotation1992677
display id: annotation1992677
version: 1
top level: Transfecti
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K157041/annotation1992677/range1992677/1

https://synbiohub.org/public/igem/BBa_K157041/annotation1992677/range1992677/1
type: Range
persistent identity: range1992677
display id: range1992677
version: 1
direction: direction/forward
top level: Transfecti
start: 21
end: 257
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1992681
display id: annotation1992681
version: 1
top level: Transfecti
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157041/annotation1992681/range1992681/1

https://synbiohub.org/public/igem/BBa_K157041/annotation1992681/range1992681/1
type: Range
persistent identity: range1992681
display id: range1992681
version: 1
direction: direction/reverse
top level: Transfecti
start: 498
end: 1196
orientation: inline

BioBrick suffix (missing a C)
type: SequenceAnnotation
persistent identity: annotation1992678
display id: annotation1992678
version: 1
top level: Transfecti
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157041/annotation1992678/range1992678/1

https://synbiohub.org/public/igem/BBa_K157041/annotation1992678/range1992678/1
type: Range
persistent identity: range1992678
display id: range1992678
version: 1
direction: direction/forward
top level: Transfecti
start: 1
end: 21
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation2064657
display id: annotation2064657
version: 1
top level: Transfecti
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157041/annotation2064657/range2064657/1

https://synbiohub.org/public/igem/BBa_K157041/annotation2064657/range2064657/1
type: Range
persistent identity: range2064657
display id: range2064657
version: 1
direction: direction/reverse
top level: Transfecti
start: 2304
end: 3164
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1992679
display id: annotation1992679
version: 1
top level: Transfecti
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K157041/annotation1992679/range1992679/1

https://synbiohub.org/public/igem/BBa_K157041/annotation1992679/range1992679/1
type: Range
persistent identity: range1992679
display id: range1992679
version: 1
direction: direction/forward
top level: Transfecti
start: 267
end: 301
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1992680
display id: annotation1992680
version: 1
top level: Transfecti
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K157041/annotation1992680/range1992680/1

https://synbiohub.org/public/igem/BBa_K157041/annotation1992680/range1992680/1
type: Range
persistent identity: range1992680
display id: range1992680
version: 1
direction: direction/reverse
top level: Transfecti
start: 394
end: 413
orientation: inline

BBa_J61003
type: ComponentDefinition
persistent identity: BBa J61003
display id: BBa_J61003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EN-Ptet-rbs-X-[GFP]-SNP "ORF Expresser"
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid for assembly of open reading frame basic parts. A Tet promoter (r0040) and strong ribosome binding site (b0034) lie between the EcoRI and XbaI sites. Insertion of an open reading frame basic part into the XbaI and PstI sites results in constitutive expression but retains basic part utilityin standard assembly.
mutable notes: N/A
mutable provenance: PCR KB001/ca1021R on pSB1A2-J01022 (250 bp, EcoRI/XbaI) sub into pSB1A2-E0040(GFP;DNA-1-5H) (EcoRI/XbaI) Product is pBca1021-E0040 ---- ca1021R Reverse b0034 XbaI AGCCATCTAGAATTTCTCCTCTTTCTCTAG KB001 Forward EcoRI to construct Ptet part-making biobrick plasmid ttctggaattcgcggccgcaactagagccaggcatc
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RBS
sequence annotation: rrnB T1 terminator
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: BBa_B0034 RBS
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: BBa_R0040 (Ptet)
sequence annotation: pBR322
sequence annotation: Ptet promoter
sequence annotation: XbaI
sequence annotation: ColE1 origin
sequence annotation: NotI
sequence annotation: BBa_B0015 (terminator)
sequence annotation: EcoRI
sequence annotation: BioBrick suffix
sequence: https://synbiohub.org/public/igem/BBa_J61003_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1975756
display id: annotation1975756
version: 1
top level: BBa_J61003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975756/range1975756/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975756/range1975756/1
type: Range
persistent identity: range1975756
display id: range1975756
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2279
end: 2290
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975747
display id: annotation1975747
version: 1
top level: BBa_J61003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975747/range1975747/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975747/range1975747/1
type: Range
persistent identity: range1975747
display id: range1975747
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2091
end: 2134
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1892281
display id: annotation1892281
version: 1
top level: BBa_J61003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892281/range1892281/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892281/range1892281/1
type: Range
persistent identity: range1892281
display id: range1892281
version: 1
direction: direction/reverse
top level: BBa_J61003
start: 957
end: 1817
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975753
display id: annotation1975753
version: 1
top level: BBa_J61003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975753/range1975753/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975753/range1975753/1
type: Range
persistent identity: range1975753
display id: range1975753
version: 1
direction: direction/forward
top level: BBa_J61003
start: 1940
end: 1959
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1892279
display id: annotation1892279
version: 1
top level: BBa_J61003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892279/range1892279/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892279/range1892279/1
type: Range
persistent identity: range1892279
display id: range1892279
version: 1
direction: direction/reverse
top level: BBa_J61003
start: 1859
end: 1887
orientation: inline

BBa_B0034 RBS
type: SequenceAnnotation
persistent identity: annotation1975755
display id: annotation1975755
version: 1
top level: BBa_J61003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975755/range1975755/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975755/range1975755/1
type: Range
persistent identity: range1975755
display id: range1975755
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2279
end: 2290
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975754
display id: annotation1975754
version: 1
top level: BBa_J61003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975754/range1975754/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975754/range1975754/1
type: Range
persistent identity: range1975754
display id: range1975754
version: 1
direction: direction/reverse
top level: BBa_J61003
start: 79
end: 98
orientation: inline

BBa_R0040 (Ptet)
type: SequenceAnnotation
persistent identity: annotation1892278
display id: annotation1892278
version: 1
top level: BBa_J61003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892278/range1892278/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892278/range1892278/1
type: Range
persistent identity: range1892278
display id: range1892278
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2217
end: 2270
orientation: inline

pBR322
type: SequenceAnnotation
persistent identity: annotation1975748
display id: annotation1975748
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975748/range1975748/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975748/range1975748/1
type: Range
persistent identity: range1975748
display id: range1975748
version: 1
direction: direction/reverse
top level: BBa_J61003
start: 183
end: 802
orientation: inline

Ptet promoter
type: SequenceAnnotation
persistent identity: annotation1975757
display id: annotation1975757
version: 1
top level: BBa_J61003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975757/range1975757/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975757/range1975757/1
type: Range
persistent identity: range1975757
display id: range1975757
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2217
end: 2270
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975752
display id: annotation1975752
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975752/range1975752/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975752/range1975752/1
type: Range
persistent identity: range1975752
display id: range1975752
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2292
end: 2297
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1892280
display id: annotation1892280
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892280/range1892280/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892280/range1892280/1
type: Range
persistent identity: range1892280
display id: range1892280
version: 1
direction: direction/forward
top level: BBa_J61003
start: 180
end: 862
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975751
display id: annotation1975751
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975751/range1975751/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975751/range1975751/1
type: Range
persistent identity: range1975751
display id: range1975751
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2064
end: 2071
orientation: inline

BBa_B0015 (terminator)
type: SequenceAnnotation
persistent identity: annotation1892277
display id: annotation1892277
version: 1
top level: BBa_J61003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892277/range1892277/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892277/range1892277/1
type: Range
persistent identity: range1892277
display id: range1892277
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2080
end: 2208
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975750
display id: annotation1975750
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975750/range1975750/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975750/range1975750/1
type: Range
persistent identity: range1975750
display id: range1975750
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2058
end: 2063
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975749
display id: annotation1975749
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975749/range1975749/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975749/range1975749/1
type: Range
persistent identity: range1975749
display id: range1975749
version: 1
direction: direction/forward
top level: BBa_J61003
start: 1
end: 21
orientation: inline

pSB1K15
type: ComponentDefinition
persistent identity: pSB1K15
display id: pSB1K15
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gateway entry vector for promoters (L4R1)
creator: Laura Deming, Joy Jiao, Adrian Slusarczyk
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _525_
m user id: 0
owner id: 5564
owning group id: 525
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Made by the 2010 MIT iGEM Team
mutable notes: To be added
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: M13 Reverse Primer
sequence annotation: M13(-20) Forward Primer
sequence annotation: attL4 Recombination Site
sequence annotation: Kanamycin
sequence annotation: rrnB T1 Transcription Terminator
sequence annotation: pUC Origin
sequence annotation: attR1 Recombination Site
sequence annotation: rrnB T2 Transcription Terminator
sequence: https://synbiohub.org/public/igem/pSB1K15_sequence/1

M13 Reverse Primer
type: SequenceAnnotation
persistent identity: annotation2113497
display id: annotation2113497
version: 1
top level: pSB1K15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1K15/annotation2113497/range2113497/1

https://synbiohub.org/public/igem/pSB1K15/annotation2113497/range2113497/1
type: Range
persistent identity: range2113497
display id: range2113497
version: 1
direction: direction/reverse
top level: pSB1K15
start: 125
end: 141
orientation: inline

M13(-20) Forward Primer
type: SequenceAnnotation
persistent identity: annotation2113496
display id: annotation2113496
version: 1
top level: pSB1K15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1K15/annotation2113496/range2113496/1

https://synbiohub.org/public/igem/pSB1K15/annotation2113496/range2113496/1
type: Range
persistent identity: range2113496
display id: range2113496
version: 1
direction: direction/forward
top level: pSB1K15
start: 2496
end: 2512
orientation: inline

attL4 Recombination Site
type: SequenceAnnotation
persistent identity: annotation2104329
display id: annotation2104329
version: 1
top level: pSB1K15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K15/annotation2104329/range2104329/1

https://synbiohub.org/public/igem/pSB1K15/annotation2104329/range2104329/1
type: Range
persistent identity: range2104329
display id: range2104329
version: 1
direction: direction/forward
top level: pSB1K15
start: 2552
end: 2648
orientation: inline

Kanamycin
type: SequenceAnnotation
persistent identity: annotation2113492
display id: annotation2113492
version: 1
top level: pSB1K15
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/pSB1K15/annotation2113492/range2113492/1

https://synbiohub.org/public/igem/pSB1K15/annotation2113492/range2113492/1
type: Range
persistent identity: range2113492
display id: range2113492
version: 1
direction: direction/forward
top level: pSB1K15
start: 354
end: 1164
orientation: inline

rrnB T1 Transcription Terminator
type: SequenceAnnotation
persistent identity: annotation2113495
display id: annotation2113495
version: 1
top level: pSB1K15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K15/annotation2113495/range2113495/1

https://synbiohub.org/public/igem/pSB1K15/annotation2113495/range2113495/1
type: Range
persistent identity: range2113495
display id: range2113495
version: 1
direction: direction/reverse
top level: pSB1K15
start: 2387
end: 2430
orientation: inline

pUC Origin
type: SequenceAnnotation
persistent identity: annotation2113493
display id: annotation2113493
version: 1
top level: pSB1K15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K15/annotation2113493/range2113493/1

https://synbiohub.org/public/igem/pSB1K15/annotation2113493/range2113493/1
type: Range
persistent identity: range2113493
display id: range2113493
version: 1
direction: direction/forward
top level: pSB1K15
start: 1284
end: 1957
orientation: inline

attR1 Recombination Site
type: SequenceAnnotation
persistent identity: annotation2104382
display id: annotation2104382
version: 1
top level: pSB1K15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K15/annotation2104382/range2104382/1

https://synbiohub.org/public/igem/pSB1K15/annotation2104382/range2104382/1
type: Range
persistent identity: range2104382
display id: range2104382
version: 1
direction: direction/forward
top level: pSB1K15
start: 1
end: 125
orientation: inline

rrnB T2 Transcription Terminator
type: SequenceAnnotation
persistent identity: annotation2113494
display id: annotation2113494
version: 1
top level: pSB1K15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K15/annotation2113494/range2113494/1

https://synbiohub.org/public/igem/pSB1K15/annotation2113494/range2113494/1
type: Range
persistent identity: range2113494
display id: range2113494
version: 1
direction: direction/reverse
top level: pSB1K15
start: 2228
end: 2255
orientation: inline

BBa_K560014
type: ComponentDefinition
persistent identity: BBa K560014
display id: BBa_K560014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BsaI Golden Gate Assembly No.3
creator: Zhang Chao
created: 2011-10-25T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
group u list: _728_
m user id: 0
owner id: 8553
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The third vector of the Golden gate series (adapted from psb1c3) This vector expedites golden gate cloning with BBa_K560012, BBa_K560013 and BBa_K560015. To make this set of vector compatible with the standard biobricks, we have maintained PstI and XbaI recognition sites. Flanking these two sites are the cleavage and recognition sites of BsaI, a kind of Type IIS restriction endonuclease. This kind of restriction endonucleases cleaves outside their recognition sites thus creating different sticky ends. If you want to assemble four biobricks at a time, you can put the biobricks in order into corresponding vector by the digestion of PstI and Xbal. Then mix these assembled vectors and a backbone (PFUS4 from TALEN golden gate kit). Under the effect of ligase and Bsal, these biobricks will have sticky ends nose to tails and ligate in order in a single reaction.
mutable notes: No
mutable provenance: Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting, Nucleic Acids Research, 2011, Vol. 39, No. 12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: PstI
sequence annotation: XbaI
sequence annotation: Prefix
sequence annotation: BsaI
sequence annotation: Suffix
sequence: https://synbiohub.org/public/igem/BBa_K560014_sequence/1

PstI
type: SequenceAnnotation
persistent identity: annotation2160722
display id: annotation2160722
version: 1
top level: BBa_K560014
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560014/annotation2160722/range2160722/1

https://synbiohub.org/public/igem/BBa_K560014/annotation2160722/range2160722/1
type: Range
persistent identity: range2160722
display id: range2160722
version: 1
direction: direction/forward
top level: BBa_K560014
start: 3
end: 8
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2160726
display id: annotation2160726
version: 1
top level: BBa_K560014
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560014/annotation2160726/range2160726/1

https://synbiohub.org/public/igem/BBa_K560014/annotation2160726/range2160726/1
type: Range
persistent identity: range2160726
display id: range2160726
version: 1
direction: direction/forward
top level: BBa_K560014
start: 2073
end: 2078
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2160721
display id: annotation2160721
version: 1
top level: BBa_K560014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K560014/annotation2160721/range2160721/1

https://synbiohub.org/public/igem/BBa_K560014/annotation2160721/range2160721/1
type: Range
persistent identity: range2160721
display id: range2160721
version: 1
direction: direction/forward
top level: BBa_K560014
start: 1
end: 24
orientation: inline

BsaI
type: SequenceAnnotation
persistent identity: annotation2160723
display id: annotation2160723
version: 1
top level: BBa_K560014
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560014/annotation2160723/range2160723/1

https://synbiohub.org/public/igem/BBa_K560014/annotation2160723/range2160723/1
type: Range
persistent identity: range2160723
display id: range2160723
version: 1
direction: direction/forward
top level: BBa_K560014
start: 14
end: 19
orientation: inline

BsaI
type: SequenceAnnotation
persistent identity: annotation2160725
display id: annotation2160725
version: 1
top level: BBa_K560014
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K560014/annotation2160725/range2160725/1

https://synbiohub.org/public/igem/BBa_K560014/annotation2160725/range2160725/1
type: Range
persistent identity: range2160725
display id: range2160725
version: 1
direction: direction/forward
top level: BBa_K560014
start: 2062
end: 2067
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2160724
display id: annotation2160724
version: 1
top level: BBa_K560014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K560014/annotation2160724/range2160724/1

https://synbiohub.org/public/igem/BBa_K560014/annotation2160724/range2160724/1
type: Range
persistent identity: range2160724
display id: range2160724
version: 1
direction: direction/forward
top level: BBa_K560014
start: 2058
end: 2080
orientation: inline

BBa_K106672
type: ComponentDefinition
persistent identity: BBa K106672
display id: BBa_K106672
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS305, Gal1P, Adh1t)
creator: Andrew Horwitz
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is an acceptor vector for AarI cloning, with A and D ends. Cloning into this plasmid will put your parts under the control of a Gal1 promoter (Gal1P) and Adh1 terminator (Adh1t). For more information regarding AarI acceptor vectors, and AarI cloning in general, please visit the UCSF 2008 wiki.
mutable notes: The pRS305 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites. Please note, the actual orientation of this vector is reversed.
mutable provenance: A modified pRS305 plasmid--Integrating yeast expression vector (LEU2 marker).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Gal1 promoter
sequence annotation: Adh1 terminator
sequence annotation: Start codon
sequence annotation: LEU2 marker
sequence annotation: f1 origin
sequence annotation: lacZalpha reporter
sequence annotation: lac promoter
sequence annotation: ampicillin resistance marker
sequence annotation: pBR322 origin
sequence annotation: https://synbiohub.org/public/igem/BBa_K106672/annotation1999705/1
sequence annotation: https://synbiohub.org/public/igem/BBa_K106672/annotation1999713/1
sequence annotation: https://synbiohub.org/public/igem/BBa_K106672/annotation1999711/1
sequence annotation: https://synbiohub.org/public/igem/BBa_K106672/annotation1999715/1
sequence annotation: https://synbiohub.org/public/igem/BBa_K106672/annotation1999708/1
sequence annotation: https://synbiohub.org/public/igem/BBa_K106672/annotation1993689/1
sequence annotation: https://synbiohub.org/public/igem/BBa_K106672/annotation1993687/1
sequence: https://synbiohub.org/public/igem/BBa_K106672_sequence/1

Gal1 promoter
type: SequenceAnnotation
persistent identity: annotation1999712
display id: annotation1999712
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999712/range1999712/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999712/range1999712/1
type: Range
persistent identity: range1999712
display id: range1999712
version: 1
direction: direction/forward
top level: BBa_K106672
start: 6172
end: 6621
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1993688
display id: annotation1993688
version: 1
top level: BBa_K106672
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993688/range1993688/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993688/range1993688/1
type: Range
persistent identity: range1993688
display id: range1993688
version: 1
direction: direction/forward
top level: BBa_K106672
start: 30
end: 215
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999714
display id: annotation1999714
version: 1
top level: BBa_K106672
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999714/range1999714/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999714/range1999714/1
type: Range
persistent identity: range1999714
display id: range1999714
version: 1
direction: direction/forward
top level: BBa_K106672
start: 6626
end: 6628
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1993684
display id: annotation1993684
version: 1
top level: BBa_K106672
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993684/range1993684/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993684/range1993684/1
type: Range
persistent identity: range1993684
display id: range1993684
version: 1
direction: direction/forward
top level: BBa_K106672
start: 1570
end: 2676
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999707
display id: annotation1999707
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999707/range1999707/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999707/range1999707/1
type: Range
persistent identity: range1999707
display id: range1999707
version: 1
direction: direction/forward
top level: BBa_K106672
start: 427
end: 733
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999706
display id: annotation1999706
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999706/range1999706/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999706/range1999706/1
type: Range
persistent identity: range1999706
display id: range1999706
version: 1
direction: direction/forward
top level: BBa_K106672
start: 246
end: 405
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999710
display id: annotation1999710
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999710/range1999710/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999710/range1999710/1
type: Range
persistent identity: range1999710
display id: range1999710
version: 1
direction: direction/forward
top level: BBa_K106672
start: 5482
end: 5511
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1993685
display id: annotation1993685
version: 1
top level: BBa_K106672
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993685/range1993685/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993685/range1993685/1
type: Range
persistent identity: range1993685
display id: range1993685
version: 1
direction: direction/forward
top level: BBa_K106672
start: 3539
end: 4399
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999709
display id: annotation1999709
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999709/range1999709/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999709/range1999709/1
type: Range
persistent identity: range1999709
display id: range1999709
version: 1
direction: direction/forward
top level: BBa_K106672
start: 4554
end: 5173
orientation: inline