BBa_J07082
BBa_J07082 Version 1 (Component)
tetR promoter+rbs+scfv-FecA 155+rbs+FecI+rbs+FecR'+terminator

BBa_J22116
BBa_J22116 Version 1 (Component)
Lac Y gene under the rec A(SOS) promoter

BBa_J07086
BBa_J07086 Version 1 (Component)
tetR promoter+rbs+scfv-FecA 567-576+rbs+FecI+rbs+FecR'+terminator

BBa_J22103
BBa_J22103 Version 1 (Component)
The Lac Y gene has been put under the rec A(SOS) promoter

BBa_K648100
BBa_K648100 Version 1 (Component)
RecA1 DH10B

BBa_K648108
BBa_K648108 Version 1 (Component)
RecA without restriction sites or recombinant activity

BBa_K648106
BBa_K648106 Version 1 (Component)
RecA1 with mutation of Arg243

BBa_K648105
BBa_K648105 Version 1 (Component)
RecA1 with mutation of Lys 286

BBa_K079016
BBa_K079016 Version 1 (Component)
RecA promoter with GFP reporter protein on a medium copy number plasmid

BBa_K648107
BBa_K648107 Version 1 (Component)
RecA1 with mutations of Arg243 and Lys286

BBa_J22121
BBa_J22121 Version 1 (Component)
Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.