BBa_K127006BBa_K127006 Version 1 (Component)B0034 C0061 B0015 I732910 E0240
BBa_S03820BBa_S03820 Version 1 (Component)B0034:E0040
BBa_S03856BBa_S03856 Version 1 (Component)E0840:I718016
BBa_S03851BBa_S03851 Version 1 (Component)J37028:E0840
BBa_S03853BBa_S03853 Version 1 (Component)I718017:E0840
BBa_S03854BBa_S03854 Version 1 (Component)I718017:E0840
BBa_S03848BBa_S03848 Version 1 (Component)E0840:J37026
BBa_K1725082BBa_K1725082 Version 1 (Component)R0040.B0034.E0040
BBa_J70476BBa_J70476 Version 1 (Component)R0010.B0030.E0040
BBa_J70544BBa_J70544 Version 1 (Component)R0010.B0032.E0040
BBa_J70480BBa_J70480 Version 1 (Component)R0010.B0034.E0040
BBa_J70477BBa_J70477 Version 1 (Component)R0010.B0031.E0040
BBa_J70481BBa_J70481 Version 1 (Component)R0010.B0035.E0040
BBa_J70545BBa_J70545 Version 1 (Component)R0010.B0033.E0040
BBa_I741026BBa_I741026 Version 1 (Component)B0030 + E0040 + B0015
BBa_K116503BBa_K116503 Version 1 (Component)GFP under control of pOmpC promoter.R0082+E0240
BBa_K235032BBa_K235032 Version 1 (Component)[R0010][E0840] (pLac+RBS+GFP+stop)
BBa_I714062BBa_I714062 Version 1 (Component)[E0040][B0015] ([GFP][Double Terminator])
B0034-phlFBBa_S05329 Version 1 (Component)B0034:K1725040
B0030-phlFBBa_S05330 Version 1 (Component)B0030:K1725040
BBa_K1529953BBa_K1529953 Version 1 (Component)K1139200 B0030 E0040 B0010 B0012
BBa_E1040BBa_E1040 Version 1 (Component)RiPS-based Protein Generator: E0040.B0015
BBa_J72046BBa_J72046 Version 1 (Component){rbs.GFP(E0040).double terminator} in BglBrick Format
BBa_K346025BBa_K346025 Version 1 (Component)PmerT promoter mutant 88+RBS(B0030)+GFP(E0040)Terminator(B0010)+Terminator(B0012)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.