CnU6CnU6_terminator Version 1 (Unknown)Organism: Cryptococcus neoformans var. neoformans JEC21
CnU6GenBank_AE017348_Region_871991_872263 Version 1 (Unknown)Organism: Cryptococcus neoformans var. neoformans JEC21
GAL7GenBank_CP003831_Region_178967_179986 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99
TEF1GenBank_CP003831_Region_375181_376053 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubi H99
UGE2GenBank_CP003831_Region_176453_177430 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99
HOG1GenBank_CP003830_Region_176667_176966 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubi H99
TEF1GenBank_CP003831_Region_377755_378976 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubi H99
GPDGenBank_AE017346_Region_940695_942557 Version 1 (Unknown)Organism: Cryptococcus neoformans var. neoformans JEC21
TRP1GenBank_CP003828_Region_1023919_1024166 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubi H99
GAL7GenBank_AE017353_Region_190704_1911746 Version 1 (Unknown)Organism: Cryptococcus neoformans var. neoformans JEC21
Cas9Cas9 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubi H99
GAL1GenBank_CP003831_Region_176110_177085 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99
URA5GenBank_CM000046_Region_302227_303005 Version 1 (Unknown)Organism: Cryptococcus neoformans var. neoformans B-3501A
URA5GenBank_CM000046_Region_301512_302226 Version 1 (Unknown)Organism: Cryptococcus neoformans var. neoformans B-3501A
URA5GenBank_CM000046_Region_303006_303540 Version 1 (Unknown)Organism: Cryptococcus neoformans var. neoformans B-3501A
APP1GenBank_CP047908_Region_1244167_1244926 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
ACT1GenBank_CP003820_Region_1242235_1243067 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
BBa_J15001BBa_J15001 Version 1 (Component)strong synthetic E. coli ribosome binding site with SacI site.
lacI tsBBa_J06501 Version 1 (Component)LacI repressor (temperature-sensitive mut 265) (+LVA)
BBa_J06801BBa_J06801 Version 1 (Component)QPI (B0034.J06501.B0015.R0011)
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_J06701BBa_J06701 Version 1 (Component)mCherry with RBS and forward terminator
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_J56004BBa_J56004 Version 1 (Component)<Composite> Tar EA400 binds to BBa_J56001
BBa_J56001BBa_J56001 Version 1 (Component)CheW with VM108 mutation for orthogonal interaction with Tar EA400
TraJRBBa_J01001 Version 1 (Component)TraJr protein (controls conjugative transfer in RP4 plasmid)
BBa_J56005BBa_J56005 Version 1 (Component)Codon-optimized aspartate receptor, the periplasmic domain of Tar
BBa_J56009BBa_J56009 Version 1 (Component)Phenylalanine-responsive receptor, signaling (cytoplasmic) domain
BBa_J56008BBa_J56008 Version 1 (Component)Phenylalanine-responsive receptor, periplasmic domain
BBa_J56006BBa_J56006 Version 1 (Component)Codon-optimized signaling domain, cytoplasmic domain of Tar
BBa_J08001BBa_J08001 Version 1 (Component)-- Death --
BBa_J46001BBa_J46001 Version 1 (Component)HSL amplifier
BBa_J56003BBa_J56003 Version 1 (Component)Tar mutated to respond to phenylalanine instead of aspartate