GFPGFP Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A H99S
HYGHygromicin_Resistance Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A strain H99O
mCherrymCherry Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A H99S strain
amdSamdS Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A strain H99O
APP1GenBank_CP047908_Region_1244167_1244926 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
ACT1GenBank_CP003820_Region_1242235_1243067 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
BBa_B0034BBa_B0034 Version 1 (Component)RBS (Elowitz 1999) -- defines RBS efficiency
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
pBad/araCBBa_I0500 Version 1 (Component)Inducible pBad/araC promoter
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
ATGATG Version 1 (Component) SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
luxrBBa_C0062 Version 1 (Component)luxR repressor/activator, (no LVA?)
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
BBa_I13453BBa_I13453 Version 1 (Component)Pbad promoter
pBADBBa_K206000 Version 1 (Component)pBAD strong
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
araCBBa_C0080 Version 1 (Component)araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
AraCBBa_R0080 Version 1 (Component)Promoter (AraC regulated)
YFPBBa_E0032 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
AraBBa_K808000 Version 1 (Component)araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
BBa_K228009BBa_K228009 Version 1 (Component)AraC protein(reversed sequence) and Pbad promoter
His-StopBBa_K133133 Version 1 (Component)His affinity tag-stop
RFC105 ABBa_K1362414 Version 1 (Component)N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
BBa_J04031BBa_J04031 Version 1 (Component)GFP w/LVA tag
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
luxIBBa_C0161 Version 1 (Component)autoinducer synthetase for AHL (no LVA)
BBa_I716463BBa_I716463 Version 1 (Component){AraC-Pbad}{rbs1-A}{ATG}{barnase no ATG}
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
BBa_I716457BBa_I716457 Version 1 (Component){AraC-Pbad}{rbs1-A}{barnase no ATG}
ECFPBBa_E0022 Version 1 (Component)enhanced cyan fluorescent protein derived from A. victoria GFP
BBa_I716462BBa_I716462 Version 1 (Component){AraC-Pbad}{rbs1-A}{ATG}{BamHI no ATG}
HABBa_I712008 Version 1 (Component)HA affinity tag