ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

HMG-CoA-R
BBa_K849000 Version 1 (Component)
3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase from Yeast

GAL1
BBa_J63006 Version 1 (Component)
yeast GAL1 promoter

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Prm +
BBa_I12007 Version 1 (Component)
Modified lambda Prm promoter (OR-3 obliterated)

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_K165002
BBa_K165002 Version 1 (Component)
Kozak sequence (yeast RBS)

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

BBa_K801012
BBa_K801012 Version 1 (Component)
ADH1 yeast terminator

BBa_I725011
BBa_I725011 Version 1 (Component)
B-hydroxy butyryl coA dehydrogenase

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

HP3 3
BBa_B0101 Version 1 (Component)
HP3 3

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

Bioplastic
BBa_K156013 Version 1 (Component)
phaB1 (acetyacetyl-CoA reductase)

c2 P22
BBa_C0053 Version 1 (Component)
c2 repressor from Salmonella phage P22 (+LVA)

BBa_K165010
BBa_K165010 Version 1 (Component)
ADH1 yeast transcription terminator

BBa_I15008
BBa_I15008 Version 1 (Component)
heme oxygenase (ho1) from Synechocystis

BBa_K309012
BBa_K309012 Version 1 (Component)
Unc-54 3' UTR (for use in C. elegans chassis)

BBa_I15009
BBa_I15009 Version 1 (Component)
phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis

BBa_K1129005
BBa_K1129005 Version 1 (Component)
Cinnamoyl-CoA reductase from Arabidopsis thaliana

BBa_K165011
BBa_K165011 Version 1 (Component)
Zif268-HIV binding sites (3)

RFC105 NN
BBa_K1362415 Version 1 (Component)
N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN

BBa_M1312
BBa_M1312 Version 1 (Component)
3-hydroxybutyryl-CoA dehydratase

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

BBa_K759005
BBa_K759005 Version 1 (Component)
phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

BBa_K1129050
BBa_K1129050 Version 1 (Component)
Cinnamoyl-CoA reductase from Arabidopsis thaliana under arabinose promoter

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)