Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0034BBa_B0034 Version 1 (Component)RBS (Elowitz 1999) -- defines RBS efficiency
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
pBad/araCBBa_I0500 Version 1 (Component)Inducible pBad/araC promoter
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
BBa_B0011BBa_B0011 Version 1 (Component)LuxICDABEG (+/-)
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
BBa_B0014BBa_B0014 Version 1 (Component)double terminator (B0012-B0011)
pT7BBa_K525998 Version 1 (Component)Promoter T7 and RBS
araCBBa_C0080 Version 1 (Component)araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
AraCBBa_R0080 Version 1 (Component)Promoter (AraC regulated)
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
BBa_K2066527BBa_K2066527 Version 1 (Component)B0034 with scar TACTAG
BBa_E0430BBa_E0430 Version 1 (Component)EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
BBa_K228000BBa_K228000 Version 1 (Component)T7ptag(T7polymerase with amber mutation)
AraBBa_K808000 Version 1 (Component)araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
BBa_K228009BBa_K228009 Version 1 (Component)AraC protein(reversed sequence) and Pbad promoter
cI+luxRBBa_R0065 Version 1 (Component)Promoter (lambda cI and luxR regulated -- hybrid)
BBa_K228878BBa_K228878 Version 1 (Component)AND GATE AraC+RBS(B0031)+T7ptag+Sal+SupD
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_K228877BBa_K228877 Version 1 (Component)AND GATE AraC+RBS(B0033)+T7ptag+Sal+SupD
BBa_K228870BBa_K228870 Version 1 (Component)AND GATE AraC+RBS(B0030)+T7ptag+Sal+SupD
BBa_K228871BBa_K228871 Version 1 (Component)AND GATE AraC+RBS(B0032)+T7ptag+Sal+SupD
BBa_K228872BBa_K228872 Version 1 (Component)AND GATE AraC+RBS(B0034)+T7ptag+Sal+SupD
BBa_K228256BBa_K228256 Version 1 (Component)AND GATE AraC+SupD+Sal+RBS(B0031)+T7ptag
BBa_K228255BBa_K228255 Version 1 (Component)AND GATE AraC+SupD+Sal+RBS(B0030)+T7ptag
BBa_K228258BBa_K228258 Version 1 (Component)AND GATE AraC+SupD+Sal+RBS(B0033)+T7ptag
BBa_K228257BBa_K228257 Version 1 (Component)AND GATE AraC+SupD+Sal+RBS(B0032)+T7ptag
BBa_K228259BBa_K228259 Version 1 (Component)AND GATE AraC+SupD+Sal+RBS(B0034)+T7ptag
BBa_K2066018BBa_K2066018 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
BBa_E0422BBa_E0422 Version 1 (Component)ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
BBa_K228874BBa_K228874 Version 1 (Component)AND GATE AraC+RBS(J61107)+T7ptag+Sal+SupD
BBa_K228873BBa_K228873 Version 1 (Component)AND GATE AraC+RBS(J61100)+T7ptag+Sal+SupD
BBa_K228876BBa_K228876 Version 1 (Component)AND GATE AraC+RBS(J44001)+T7ptag+Sal+SupD
BBa_K228851BBa_K228851 Version 1 (Component)Sal+supD+terminator
BBa_K228875BBa_K228875 Version 1 (Component)AND GATE AraC+RBS(J61127)+T7ptag+Sal+SupD
BBa_K228263BBa_K228263 Version 1 (Component)AND GATE AraC+SupD+Sal+RBS(J61127)+T7ptag
BBa_K228260BBa_K228260 Version 1 (Component)AND GATE AraC+SupD+Sal+RBS(J44001)+T7ptag
BBa_K228261BBa_K228261 Version 1 (Component)AND GATE AraC+SupD+Sal+RBS(J61100)+T7ptag