Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
BBa_B0034BBa_B0034 Version 1 (Component)RBS (Elowitz 1999) -- defines RBS efficiency
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
LacIBBa_R0010 Version 1 (Component)promoter (lacI regulated)
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
lacIBBa_C0012 Version 1 (Component)lacI repressor from E. coli (+LVA)
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
lacI+pLBBa_R0011 Version 1 (Component)Promoter (lacI regulated, lambda pL hybrid)
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
LacILacI Version 1 (Component) BBa_B0014BBa_B0014 Version 1 (Component)double terminator (B0012-B0011)
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
P(Lac) IQBBa_I14032 Version 1 (Component)promoter P(Lac) IQ
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
pT7BBa_K525998 Version 1 (Component)Promoter T7 and RBS
BBa_J33207BBa_J33207 Version 1 (Component)lac promoter and lacZ
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
BBa_E0430BBa_E0430 Version 1 (Component)EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
BBa_K2066527BBa_K2066527 Version 1 (Component)B0034 with scar TACTAG
BBa_K228000BBa_K228000 Version 1 (Component)T7ptag(T7polymerase with amber mutation)
lacZ-alphaBBa_I732006 Version 1 (Component)lacZ alpha fragment
lacIBBa_Q04121 Version 1 (Component)QPI (B0034.C0012.B0015.R0011)
cI+luxRBBa_R0065 Version 1 (Component)Promoter (lambda cI and luxR regulated -- hybrid)
BBa_E0422BBa_E0422 Version 1 (Component)ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_E0420BBa_E0420 Version 1 (Component)ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
LacIBBa_I732100 Version 1 (Component)LacI
BBa_K228855BBa_K228855 Version 1 (Component)AND GATE Sal+SupD+LacP+RBS(B0034)+T7ptag
BBa_K228854BBa_K228854 Version 1 (Component)AND GATE Sal+SupD+LacP+RBS(B0032)+T7ptag
BBa_K228860BBa_K228860 Version 1 (Component)AND GATE Sal+SupD+LacP+RBS(B0031)+T7ptag
BBa_K228859BBa_K228859 Version 1 (Component)AND GATE Sal+SupD+LacP+RBS(B0033)+T7ptag
BBa_K228853BBa_K228853 Version 1 (Component)AND GATE Sal+SupD+LacP+RBS(B0030)+T7ptag
BBa_K2066018BBa_K2066018 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
BBa_K228851BBa_K228851 Version 1 (Component)Sal+supD+terminator
BBa_K228872BBa_K228872 Version 1 (Component)AND GATE AraC+RBS(B0034)+T7ptag+Sal+SupD
T7 RBSBBa_K1362090 Version 1 (Component)strong T7 RBS
BBa_K228877BBa_K228877 Version 1 (Component)AND GATE AraC+RBS(B0033)+T7ptag+Sal+SupD
BBa_K228870BBa_K228870 Version 1 (Component)AND GATE AraC+RBS(B0030)+T7ptag+Sal+SupD
BBa_K228871BBa_K228871 Version 1 (Component)AND GATE AraC+RBS(B0032)+T7ptag+Sal+SupD
BBa_K228878BBa_K228878 Version 1 (Component)AND GATE AraC+RBS(B0031)+T7ptag+Sal+SupD
tetRBBa_Q04400 Version 1 (Component)QPI (B0034.C0040.B0015.R0040)