Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
ATGATG Version 1 (Component) scarBBa_K112999 Version 1 (Component)BBb1 scar sequence
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
pT7BBa_K525998 Version 1 (Component)Promoter T7 and RBS
cI+luxRBBa_R0065 Version 1 (Component)Promoter (lambda cI and luxR regulated -- hybrid)
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
His-StopBBa_K133133 Version 1 (Component)His affinity tag-stop
BBa_J04031BBa_J04031 Version 1 (Component)GFP w/LVA tag
BBa_K2116060BBa_K2116060 Version 1 (Component)AND gate bxb1 (fast tag)
HABBa_I712008 Version 1 (Component)HA affinity tag
NLSBBa_J176008 Version 1 (Component)NLS
EcoRIEcoRI Version 1 (Component) NheINheI Version 1 (Component) PacIPacI Version 1 (Component) SpeISpeI Version 1 (Component) BBa_K105012BBa_K105012 Version 1 (Component)10 aa flexible protein domain linker
PESTBBa_J176018 Version 1 (Component)PEST
BBa_M0052BBa_M0052 Version 1 (Component)AANDENYADAS (moderately fast) SsrA degradation tag.
linkerBBa_K133132 Version 1 (Component)8 aa protein domain linker
BBa_K2116030BBa_K2116030 Version 1 (Component)bxb1 integrase with moderately fast degradation tag
BBa_K844000BBa_K844000 Version 1 (Component)10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
NLSmycSTOPBBa_J176031 Version 1 (Component)NLS myc stop
RGD-His-StBBa_K133036 Version 1 (Component)RGD-His affinity tag-Stop
BBa_K2116009BBa_K2116009 Version 1 (Component)bxb1 integrase, with very fast degradation tag
BBa_M0051BBa_M0051 Version 1 (Component)AANDENYNYADAS. (Fast) SsrA degradation tag.
Bxb1_IntBBa_K907000 Version 1 (Component)Mycobacterium Phage Bxb1 gp35, DNA integrase
BBa_J33207BBa_J33207 Version 1 (Component)lac promoter and lacZ
LAABBa_M0050 Version 1 (Component)AANDENYALAA. (Very fast) SsrA degradation tag.
G-His6(N)BBa_K1362430 Version 1 (Component)C-terminal His6 (N-Intein)
BBa_K416003BBa_K416003 Version 1 (Component)Yeast Secretion Tag
RBS-GFP-TeBBa_I763020 Version 1 (Component)RBS-GFP (+LVA Tag)-Terminators
BBa_K851002BBa_K851002 Version 1 (Component)pBAD-pXyl AND Gate
BBa_J70042BBa_J70042 Version 1 (Component)6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)
BBa_K2144009BBa_K2144009 Version 1 (Component)Sequence for His6-tag and LPXTGG-tag
polyABBa_J176009 Version 1 (Component)polyA2
rhIIBBa_C0070 Version 1 (Component)autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
BBa_K608002BBa_K608002 Version 1 (Component)strong Promoter and strong RBS
BBa_J70044BBa_J70044 Version 1 (Component)6his part for use with BioScaffold parts (G)GATCC(glycine serine)-6his-TAA
His6BBa_K1362431 Version 1 (Component)N-terminal His6 (C-Intein)
BBa_J04051BBa_J04051 Version 1 (Component)RFP with tag