BBa_K896902
BBa_K896902 Version 1 (Component)
Against aging!!

BBa_K896917
BBa_K896917 Version 1 (Component)
Against aging!!

BBa_K748002
BBa_K748002 Version 1 (Component)
Truncated lysostaphin coding sequence. Lysostaphin has has a specific lytic action against S.aureus.

BBa_K320001
BBa_K320001 Version 1 (Component)
Immunotoxin against P.Berghei

BBa_K382019
BBa_K382019 Version 1 (Component)
ihpRNA construct against Arabidopsis Ger3 allergen

BBa_K382017
BBa_K382017 Version 1 (Component)
ihpRNA construct against Arabidopsis LTP Allergen

BBa_K382015
BBa_K382015 Version 1 (Component)
ihpRNA construct against Arabidopsis LTP Allergen

BBa_K1351017
BBa_K1351017 Version 1 (Component)
SdpI with RBS: Immunity against the cannibalsim toxin sdpC of <i>B. subtilis</i>

BBa_K1833012
BBa_K1833012 Version 1 (Component)
lac promoter -> scFv against Human VEGF-A

BBa_K382014
BBa_K382014 Version 1 (Component)
ihpRNA construct against Arabidopsis Bet v 1 allergen

BBa_I723125
BBa_I723125 Version 1 (Component)
Test reporter again

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.