Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
cI lamBBa_R0051 Version 1 (Component)promoter (lambda cI regulated)
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
OmpABBa_K103006 Version 1 (Component)OmpA outer membrane protein A fused to linker; displays proteins on cell surface
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_K1811333BBa_K1811333 Version 1 (Component)CUP1 metallothionein fused to LamB on outer membrane
CD4 signalBBa_I712009 Version 1 (Component)CD4 signal peptide; localizes to plasma membrane
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
BBa_I746200BBa_I746200 Version 1 (Component)FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.
cI lamBBa_R1051 Version 1 (Component)Promoter, Standard (lambda cI regulated)<br>
BBa_K1051105BBa_K1051105 Version 1 (Component)TOM40;targeting peptide to mitochondria outer membrane
BBa_K1811666BBa_K1811666 Version 1 (Component)CUP1 metallothionein fused to LamB with promoter and RBS
PelBBBa_J32015 Version 1 (Component)PelB leader sequence; directs protein to E. coli periplasmic membrane
BBa_K1321151BBa_K1321151 Version 1 (Component)metallothionein Fmt fused to CBDclos
BBa_K1321121BBa_K1321121 Version 1 (Component)Smt metallothionein fused to CBDclos
BBa_K1321152BBa_K1321152 Version 1 (Component)metallothionein Fmt fused to CBDcex
BBa_K1321154BBa_K1321154 Version 1 (Component)CBDclos fused to metallothionein Fmt
lamBBBa_K137112 Version 1 (Component)lamB
BBa_K1321120BBa_K1321120 Version 1 (Component)SmtA metallothionein fused to linker-dCBD
oprFBBa_K1172501 Version 1 (Component)Outer membrane porin OprF from Pseudomonas fluorescens
BBa_J36837BBa_J36837 Version 1 (Component)Outer membrane protein A, aa 46-159
BBa_J36836BBa_J36836 Version 1 (Component)Outer membrane protein A, aa 46-66
BBa_I746009BBa_I746009 Version 1 (Component)AIP generator + outer membrane permeability
BBa_J44001BBa_J44001 Version 1 (Component)Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
OmpA_LBBa_K257003 Version 1 (Component)Outer membrane protein A (partial) fused to linker
BBa_K1051106BBa_K1051106 Version 1 (Component)targeting peptide to mitochondria outer membrane
BBa_K1051104BBa_K1051104 Version 1 (Component)VDAC1;targeting peptide to mitochondria outer membrane
BBa_K1321011BBa_K1321011 Version 1 (Component)SmtA metallothionein in Freiburg format (RFC[25])
BBa_K1321159BBa_K1321159 Version 1 (Component)Fmt metallothionein with T7 promoter
BBa_M1028BBa_M1028 Version 1 (Component)tolC-Outer membrane protein
BBa_K1321150BBa_K1321150 Version 1 (Component)Metallothionein Fmt fused to linker-dCBD
BBa_K1321342BBa_K1321342 Version 1 (Component)sfGFP fused to CBDcex in RFC 25
BBa_K331008BBa_K331008 Version 1 (Component)Outer Membrane Protein OmpA
BBa_K607008BBa_K607008 Version 1 (Component)reverse lasB promoter
BBa_K1393003BBa_K1393003 Version 1 (Component)outer membrane porin protein C from Escherichia coli BL21(DE3)
myristoilaBBa_I712026 Version 1 (Component)myristoilation signal sequence; anchors protein to membrane