Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

BBa_K1510929
BBa_K1510929 Version 1 (Component)
Circuit injected in Streptococcus mutans that allows S.m. yield N-Acyl homoserine lactone.

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K1331003
BBa_K1331003 Version 1 (Component)
RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6

rhII
BBa_C0070 Version 1 (Component)
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL

BBa_K2033011
BBa_K2033011 Version 1 (Component)
N-Acyl Homoserine Lactone (AHL) Modular Sender Vector

BBa_K1718003
BBa_K1718003 Version 1 (Component)
N-acyl-homoserine-lactone inducible LuxPr promoter generating RFP

BBa_K1321340
BBa_K1321340 Version 1 (Component)
Double CBD (dCBD) with N-terminal linker

AAR
BBa_K590032 Version 1 (Component)
Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942

BBa_K663000
BBa_K663000 Version 1 (Component)
Homoserine lactone sensor

lacZ a
BBa_E0033 Version 1 (Component)
LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)

BBa_K2033008
BBa_K2033008 Version 1 (Component)
N-3-oxo-tetradecanoyl-L-Homoserine lactone* Sender- SinI

GC Optimised Sequence Pull
GC_Optimised Version 1 (Activity)
Used to indicate that a sequence was GC optimised after pulling from the source and before submission

BBa_K2033000
BBa_K2033000 Version 1 (Component)
N-dodecanoyl-L-homoserine lactone (C(12)-HSL) Sender- AubI

BBa_M36268
BBa_M36268 Version 1 (Component)
LasR-N-(3-oxododecanoyl)-homoserine lactone qsc102 sensor

BBa_K1676076
BBa_K1676076 Version 1 (Component)
Lactate Permease

NpuDnaE(N)
BBa_K1362400 Version 1 (Component)
NpuDnaE N-Intein cloning piece

BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

BBa_K663001
BBa_K663001 Version 1 (Component)
Wintergreen-generator on an homoserine lactone responsive promoter

CBDcipA
BBa_K1615111 Version 1 (Component)
CBDcipA with N and C-terminal linker in RFC25

BBa_K1510816
BBa_K1510816 Version 1 (Component)
A Steptococcus Mutants adhesive complex.

BBa_K249008
BBa_K249008 Version 1 (Component)
YFP with N-terminal Arganine Tag

LacI
BBa_K143033 Version 1 (Component)
LacI (Lva^-, N-terminal deletion) regulatory protein

BBa_M41016
BBa_M41016 Version 1 (Component)
Calcium sensor from Streptococcus mutans

BBa_K1695000
BBa_K1695000 Version 1 (Component)
Tightly-regulated lactose-inducible promoter

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

BBa_K861020
BBa_K861020 Version 1 (Component)
FadE ,acyl-CoA dehydrogenase

BBa_K1321002
BBa_K1321002 Version 1 (Component)
Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25

BBa_K1051000
BBa_K1051000 Version 1 (Component)
Stop codon free RFP in RFC[23] standard

BBa_K1510409
BBa_K1510409 Version 1 (Component)
A Steptococcus Mutants adhesive complex.

BBa_K861010
BBa_K861010 Version 1 (Component)
FadD, an inner membrane-associated acyl-CoA synthase

BBa_K1510114
BBa_K1510114 Version 1 (Component)
C16 is a signal peptide that binds to the surface of Streptococcus Mutants.

BBa_K1321003
BBa_K1321003 Version 1 (Component)
Re-entry of BBa_K863101, Cellulose Binding Domain CBDcex in rfc25

BBa_K663002
BBa_K663002 Version 1 (Component)
Wintergreen and GFP-generators on an homoserine lactone responsive promoter

BBa_K1423000
BBa_K1423000 Version 1 (Component)
BCLA N-terminal Domain

BBa_K1159101
BBa_K1159101 Version 1 (Component)
N-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

BBa_K400628
BBa_K400628 Version 1 (Component)
mAAA (EcoRI site mutated Aryl Acyl-Amidase)