SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Cre
BBa_J61047 Version 1 (Component)
Cre DNA recombinase

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

BBa_K809601
BBa_K809601 Version 1 (Component)
GAL promoter (kozak) without start codon ATG

BBa_M36009
BBa_M36009 Version 1 (Component)
5' Bicistronic UTR (strong), does not include ATG start

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

pRE
BBa_K116603 Version 1 (Component)
pRE promoter from λ phage

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

BBa_I716213
BBa_I716213 Version 1 (Component)
Cre (TTG start)

T7start
BBa_J18912 Version 1 (Component)
T7 promoter + RBS + START

BBa_J36841
BBa_J36841 Version 1 (Component)
Streptavidin, wild-type core + His6 tag (no start codon)

RE
BBa_J3101 Version 1 (Component)
Recombinational Enhancer (RE) for Hin/Hix inverting

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

NLS
BBa_J176008 Version 1 (Component)
NLS

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

PEST
BBa_J176018 Version 1 (Component)
PEST

T7rnap
BBa_I716103 Version 1 (Component)
T7rnap (T7 RNA Polymerase) - ATG Start Codon

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

BBa_K1333002
BBa_K1333002 Version 1 (Component)
M13 bacteriophage g3p with start codon of "ATG" and a FLAG tag on C-terminal

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

NLSmycSTOP
BBa_J176031 Version 1 (Component)
NLS myc stop

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

BBa_K2066518
BBa_K2066518 Version 1 (Component)
mCherry (with start codon)

cre
BBa_K112102 Version 1 (Component)
Cre enzyme without start or stop codons

BBa_M36001
BBa_M36001 Version 1 (Component)
5' Bicistronic UTR (medium), does not include ATG start

BBa_K1150016
BBa_K1150016 Version 1 (Component)
HA tag

BBa_M36282
BBa_M36282 Version 1 (Component)
5' Bicistronic UTR, includes ATG start codon

BBa_K284031
BBa_K284031 Version 1 (Component)
Cre Recombinase without start codon

BBa_K299801
BBa_K299801 Version 1 (Component)
standard biobrick scar (if next sequence does NOT start with ATG)

BBa_J36843
BBa_J36843 Version 1 (Component)
Streptavidin, single-chain dimer + His6 tag (no start codon)

BBa_K1583059
BBa_K1583059 Version 1 (Component)
His-tag without start codon

BBa_K112100
BBa_K112100 Version 1 (Component)
Cre enzyme without start codon

STAR
BBa_K1893013 Version 1 (Component)
Small transcription activating RNA (STAR)

BBa_K844006
BBa_K844006 Version 1 (Component)
Spider Silk 1x Subunit "W" with Met (ATG) start codon

CHE
BBa_K256023 Version 1 (Component)
Cholesteryl Esterase (CHE)

RFC105 A
BBa_K1362440 Version 1 (Component)
N-terminal start overhang (T)(A)-GATG=RBS+Start RFC[105] A

BBa_M36724
BBa_M36724 Version 1 (Component)
5' Bicistronic UTR (strong), does not include ATG start

CRP
BBa_J58112 Version 1 (Component)
CRP protein

BBa_I716210
BBa_I716210 Version 1 (Component)
BamHI endonuclease without start codon

BCRBS
BBa_M36293 Version 1 (Component)
5' Bicistronic UTR, includes ATG start

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

BBa_K323217
BBa_K323217 Version 1 (Component)
T7 promoter with start codon and hexahistidine tag