Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
tetRBBa_C0040 Version 1 (Component)tetracycline repressor from transposon Tn10 (+LVA)
BBa_J04450BBa_J04450 Version 1 (Component)RFP Coding Device
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
luxIBBa_C0061 Version 1 (Component)autoinducer synthetase for AHL
BBa_C0261BBa_C0261 Version 1 (Component)AHL-making Enzyme, luxI (+RBS)
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
aiiABBa_C0060 Version 1 (Component)autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
BBa_K809601BBa_K809601 Version 1 (Component)GAL promoter (kozak) without start codon ATG
BBa_K844000BBa_K844000 Version 1 (Component)10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
BBa_K1051003BBa_K1051003 Version 1 (Component)Stop codon free GFP in RFC[23] standard
creBBa_K112102 Version 1 (Component)Cre enzyme without start or stop codons
CreBBa_J61047 Version 1 (Component)Cre DNA recombinase
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
BBa_K1051000BBa_K1051000 Version 1 (Component)Stop codon free RFP in RFC[23] standard
PspaSPspaS_core Version (Component) RFC105 ZBBa_K1362421 Version 1 (Component)C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z
EcoRIEcoRI Version 1 (Component) NheINheI Version 1 (Component) PacIPacI Version 1 (Component) SpeISpeI Version 1 (Component) BBa_K112113BBa_K112113 Version 1 (Component)Ligase without start or stop codons
His-StopBBa_K133133 Version 1 (Component)His affinity tag-stop
dCas 9BBa_K1051800 Version 1 (Component)protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.
BBa_K112108BBa_K112108 Version 1 (Component)BglII endonuclease without start or stop codons
BBa_K112100BBa_K112100 Version 1 (Component)Cre enzyme without start codon
BBa_I716210BBa_I716210 Version 1 (Component)BamHI endonuclease without start codon
BBa_K112101BBa_K112101 Version 1 (Component)Cre enzyme without stop codon
BBa_K112105BBa_K112105 Version 1 (Component)BamHI endonuclease without start or stop codons
RGD-His-StBBa_K133036 Version 1 (Component)RGD-His affinity tag-Stop
RFC105 ABBa_K1362414 Version 1 (Component)N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
BBa_M36117BBa_M36117 Version 1 (Component)Stop Codon
BBa_K284031BBa_K284031 Version 1 (Component)Cre Recombinase without start codon
BBa_K1332002BBa_K1332002 Version 1 (Component)Histidine tag (8 AA) and RFP (without stop codon)
BBa_I746916BBa_I746916 Version 1 (Component)superfolder GFP coding sequence
BBa_K1051004BBa_K1051004 Version 1 (Component)Stop codon free mOrange in RFC[23] standard
BBa_K1582030BBa_K1582030 Version 1 (Component)mTagBFP without stop codon
BBa_K1582037BBa_K1582037 Version 1 (Component)LC without stop codon
BBa_K1582039BBa_K1582039 Version 1 (Component)Thc_Cut1 without stop codon
BBa_K1582038BBa_K1582038 Version 1 (Component)FsC without stop codon
BBa_K1051053BBa_K1051053 Version 1 (Component)K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 terminator)
BBa_K2066518BBa_K2066518 Version 1 (Component)mCherry (with start codon)
lacZ-alphaBBa_I732006 Version 1 (Component)lacZ alpha fragment