Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

BBa_K823003
BBa_K823003 Version 1 (Component)
P_veg

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

pSB1C3
pSB1C3 Version 1 (Component)
High copy BioBrick assembly plasmid

Scar 25
BBa_B0105 Version 1 (Component)
RFC 25 Scar Sequence

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

BBa_K823013
BBa_K823013 Version 1 (Component)
Anderson promoter J23117

BBa_J56012
BBa_J56012 Version 1 (Component)
Invertible sequence of dna includes Ptrc promoter

BBa_K523023
BBa_K523023 Version 1 (Component)
PlacLacZ + EYFP

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

BBa_K1930003
BBa_K1930003 Version 1 (Component)
Encrypted message sequence in integration plasmid (BBa_K823023)

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

BBa_K823043
BBa_K823043 Version 1 (Component)
alternative sigma factor ecf41

pSBBs4S
BBa_K823024 Version 1 (Component)
pSB_Bs4S-P<i>_xyl</i>: Integrative expression vector for <i>Bacillus subtilis</i

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_K223043
BBa_K223043 Version 1 (Component)
RALDH II gene: Converts retinal into retinoic acid

pSBBs0K
BBa_K823026 Version 1 (Component)
pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector; IPTG inducible

BBa_K223023
BBa_K223023 Version 1 (Component)
trp-operon (promoter-operator) sequence

BBa_K223022
BBa_K223022 Version 1 (Component)
IL-6 + HlyA

scar
BBa_K112999 Version 1 (Component)
BBb1 scar sequence

pSBBs1C
BBa_K823023 Version 1 (Component)
pSB_Bs1C: Empty backbone for integration into <i>Bacillus subtilis</i> <i>amyE</i> locus

BBa_K1820023
BBa_K1820023 Version 1 (Component)
FspI + terminator

pSBBs1C
BBa_K823021 Version 1 (Component)
pSB_Bs1C-<i>lacZ</i> (lacZ reporter vector for <i>B. subtilis</i>)

pSBBs3C
BBa_K823025 Version 1 (Component)
pSB_Bs3C-<i>luxABCDE</i> (lux reporter vector for B.subtilis)

BBa_K323023
BBa_K323023 Version 1 (Component)
mCerulean

BBa_K823028
BBa_K823028 Version 1 (Component)
luc luciferase + RBS, codon optimized for Bacillus subtilis

BBa_K823029
BBa_K823029 Version 1 (Component)
mKate2, a red monomeric fluorescent protein, B. subtilis optimized

BBa_K827023
BBa_K827023 Version 1 (Component)
artificial designed terminator

RFC 37
BBa_K245009 Version 1 (Component)
RFC 37 Scar Sequence

pSBBs2E
BBa_K823027 Version 1 (Component)
pSB_Bs2E: Empty backbone for integration into Bacillus subtilis lacA locus

pSBBs4S
BBa_K823022 Version 1 (Component)
pSB_Bs4S: Empty backbone for integration into <i/>Bacillus subtilis thrC</i> locus

BBa_K823020
BBa_K823020 Version 1 (Component)
chloramphenicol acetyltransferase (cat)