Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

iGEM to SBOL conversion
igem2sbol Version 1 (Activity)
Conversion of the iGEM parts registry to SBOL2.1

SyBiOntKB to SBOL conversion
SyBiOntKB2sbol Version 1 (Activity)
Conversion of the Synthetic Biology Ontology knowledge base to SBOL 2.1

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

BBa_K543002
BBa_K543002 Version 1 (Component)
HNS mutant T108I

BBa_K543003
BBa_K543003 Version 1 (Component)
HNS mutant T108I protein generator

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

GC Optimised Sequence Pull
GC_Optimised Version 1 (Activity)
Used to indicate that a sequence was GC optimised after pulling from the source and before submission

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

BBa_K1639005
BBa_K1639005 Version 1 (Component)
H-NS(T108I)

BBa_B0053
BBa_B0053 Version 1 (Component)
Terminator (His)

BBa_K082005
BBa_K082005 Version 1 (Component)
LacI-Mutant

His
BBa_K157011 Version 1 (Component)
His affinity tag; Freiburg standard

BBa_I746200
BBa_I746200 Version 1 (Component)
FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.

BBa_J23151
BBa_J23151 Version 1 (Component)
1bp mutant from J23114

mRdRP
BBa_K1442101 Version 1 (Component)
RdRP Mutant

BBa_J23150
BBa_J23150 Version 1 (Component)
1bp mutant from J23107

lacZ a
BBa_E0033 Version 1 (Component)
LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)

BBa_J202014
BBa_J202014 Version 1 (Component)
PBAD Mutant#15

BBa_J202011
BBa_J202011 Version 1 (Component)
PBAD Mutant#12

BBa_J202013
BBa_J202013 Version 1 (Component)
PBAD Mutant#14

BBa_J202005
BBa_J202005 Version 1 (Component)
PBAD Mutant#6

BBa_J202008
BBa_J202008 Version 1 (Component)
PBAD Mutant#9

BBa_J202000
BBa_J202000 Version 1 (Component)
PBAD mutant#1

BBa_J202004
BBa_J202004 Version 1 (Component)
PBAD Mutant#5

BBa_J202010
BBa_J202010 Version 1 (Component)
PBAD Mutant#11

BBa_J202003
BBa_J202003 Version 1 (Component)
PBAD Mutant#4

BBa_J202002
BBa_J202002 Version 1 (Component)
PBAD Mutant#3

BBa_J202012
BBa_J202012 Version 1 (Component)
PBAD Mutant#13

BBa_J202009
BBa_J202009 Version 1 (Component)
PBAD Mutant#10

BBa_J202006
BBa_J202006 Version 1 (Component)
PBAD Mutant#7

BBa_K1763421
BBa_K1763421 Version 1 (Component)
PCquad Mutant 10

BBa_K613013
BBa_K613013 Version 1 (Component)
TetR V36F mutant

His-Scaffo
BBa_K1602027 Version 1 (Component)
Protein scaffold with N-terminal HisTag