Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

Cat
BBa_I14033 Version 1 (Component)
P(Cat)

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

Hin
BBa_J31000 Version 1 (Component)
DNA-invertase Hin from Salmonella typhimurium

RE
BBa_J3101 Version 1 (Component)
Recombinational Enhancer (RE) for Hin/Hix inverting

NLS
BBa_J176008 Version 1 (Component)
NLS

BBa_K1223006
BBa_K1223006 Version 1 (Component)
His-Tag

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

BBa_B0053
BBa_B0053 Version 1 (Component)
Terminator (His)

PEST
BBa_J176018 Version 1 (Component)
PEST

pLux/Tet
BBa_K176000 Version 1 (Component)
pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

His-Scaffo
BBa_K1602027 Version 1 (Component)
Protein scaffold with N-terminal HisTag

NLSmycSTOP
BBa_J176031 Version 1 (Component)
NLS myc stop

BBa_K233307
BBa_K233307 Version 1 (Component)
TorA- This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT)

BBa_K233306
BBa_K233306 Version 1 (Component)
YcdB - This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT)

BBa_K726003
BBa_K726003 Version 1 (Component)
T7 promoter + lac operator + His tag

BBa_K1861102
BBa_K1861102 Version 1 (Component)
His-Tag

BBa_K1150016
BBa_K1150016 Version 1 (Component)
HA tag

BBa_K1189009
BBa_K1189009 Version 1 (Component)
Beta-lactamase with His Tag

BBa_I13600
BBa_I13600 Version 1 (Component)
Tet with CFP reporter (without LVA tag)

GC Optimised Sequence Pull
GC_Optimised Version 1 (Activity)
Used to indicate that a sequence was GC optimised after pulling from the source and before submission

BBa_K1123012
BBa_K1123012 Version 1 (Component)
His-Tag and Thrombine Cleavage Site

Kat
BBa_I14034 Version 1 (Component)
P(Kat)

BBa_K2144009
BBa_K2144009 Version 1 (Component)
Sequence for His6-tag and LPXTGG-tag

eGFP+His
BBa_K193202 Version 1 (Component)
EGFP(enhanced Green Fluorescent Protein) with His tag

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

Tat PROM
BBa_K895000 Version 1 (Component)
Eco_Ptat2

His6
BBa_K1362431 Version 1 (Component)
N-terminal His6 (C-Intein)

BBa_I13602
BBa_I13602 Version 1 (Component)
Tet operator with CFP reporter (with LVA tag) [R/Tc+]

BBa_J70048
BBa_J70048 Version 1 (Component)
6his part for use with BioScaffold parts ATG-GGATCC (glycine serine)-6 His-TAA

HIC genera
BBa_K1602020 Version 1 (Component)
Inducible generator of Humicola insolens cutinase (HIC) with N-terminal His-Tag