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Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Public

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Public

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

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BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

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BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

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BBa_B0015
BBa_B0015 Version 1 (Component)
double terminator (B0010-B0012)

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BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

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BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

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BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

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BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

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BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

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BBa_B0011
BBa_B0011 Version 1 (Component)
LuxICDABEG (+/-)

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BBa_B0014
BBa_B0014 Version 1 (Component)
double terminator (B0012-B0011)

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Scar 25
BBa_B0105 Version 1 (Component)
RFC 25 Scar Sequence

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BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

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mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

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BBa_B0025
BBa_B0025 Version 1 (Component)
double terminator (B0015), reversed

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tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

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Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

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BBa_B0016
BBa_B0016 Version 1 (Component)
Terminator (T7 RNAP specific, T_Phi)

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BBa_K1051006
BBa_K1051006 Version 1 (Component)
Saccharomycescerevisiae TBY-1 terminator with stopcodon

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BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

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BBa_K115001
BBa_K115001 Version 1 (Component)
RNA thermometer (ROSE)

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BBa_K1051000
BBa_K1051000 Version 1 (Component)
Stop codon free RFP in RFC[23] standard

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BBa_K1051008
BBa_K1051008 Version 1 (Component)
S. cerevisiae CAA terminator with stopcodon

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BBa_B0017
BBa_B0017 Version 1 (Component)
double terminator (B0010-B0010)

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cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

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BBa_B0024
BBa_B0024 Version 1 (Component)
double terminator (B0012-B0011), reversed

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mCherry
BBa_J06505 Version 1 (Component)
monomeric RFP optimized for bacteria

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eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

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BBa_B0035
BBa_B0035 Version 1 (Component)
RBS (B0030 derivative)

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spacer
BBa_B0040 Version 1 (Component)
Spacer.1 (generic)

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BBa_K1051004
BBa_K1051004 Version 1 (Component)
Stop codon free mOrange in RFC[23] standard

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luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

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BBa_K1150001
BBa_K1150001 Version 1 (Component)
VP16

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lacI ts
BBa_J06501 Version 1 (Component)
LacI repressor (temperature-sensitive mut 265) (+LVA)

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BBa_K1051001
BBa_K1051001 Version 1 (Component)
Stop codon free ECFP in RFC[23] standard

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BBa_K1051002
BBa_K1051002 Version 1 (Component)
Stop codon free EYFP in RFC[23] standard

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BBa_B0013
BBa_B0013 Version 1 (Component)
TE from coliphage T7 (+/-)

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BBa_J04051
BBa_J04051 Version 1 (Component)
RFP with tag

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mRFP1
BBa_J04650 Version 1 (Component)
E1010.B0015

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cI857
BBa_J06503 Version 1 (Component)
Lambda cI857 (Lambda II) (RBS- LVA+)

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BBa_K1151008
BBa_K1151008 Version 1 (Component)
RFP nickel regulated

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BBa_K1051009
BBa_K1051009 Version 1 (Component)
e.coli terminator ilvGEDA_T with stop codon

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BBa_B0053
BBa_B0053 Version 1 (Component)
Terminator (His)

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BBa_K1151006
BBa_K1151006 Version 1 (Component)
HpNikR coding device

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ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

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hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

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BBa_K1151005
BBa_K1151005 Version 1 (Component)
pUreA

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BBa_K1151001
BBa_K1151001 Version 1 (Component)
Histidine-rich metal-binding protein

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