Cas9Cas9 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubi H99
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
GFPBBa_E0040 Version 1 (Component)green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Amp CasBBa_I13800 Version 1 (Component)Ampicillin Resistance Cassette
tetRBBa_C0040 Version 1 (Component)tetracycline repressor from transposon Tn10 (+LVA)
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
luxIBBa_C0061 Version 1 (Component)autoinducer synthetase for AHL
BBa_K1773005BBa_K1773005 Version 1 (Component)I-F type CRISPR-Cas Cascade complex
tetA(C)fBBa_J31007 Version 1 (Component)tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
BBa_K1773003BBa_K1773003 Version 1 (Component)I-F type CRISPR-Cas Cas3 gene
BBa_K1773000BBa_K1773000 Version 1 (Component)I-F type CRISPR-Cas Cascade complex
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
BBa_K1773001BBa_K1773001 Version 1 (Component)I-F type CRISPR-Cas Cascade complex
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
CatBBa_I14033 Version 1 (Component)P(Cat)
BBa_K1773002BBa_K1773002 Version 1 (Component)I-F type CRISPR-Cas Cas3 protein
CouplingCoupling Version 1 (Component)Translational coupler
CdBBa_K1088060 Version 1 (Component)I-E type Cascade complex
BBa_K1773004BBa_K1773004 Version 1 (Component)I-F type CRISPR-Cas homogenous CRISPR region
aiiABBa_C0060 Version 1 (Component)autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
BBa_K1445001BBa_K1445001 Version 1 (Component)Endogenous Type II CRISPR-Cas9 phagemid
CDSCDS Version (Component) lacZ-alphaBBa_I732006 Version 1 (Component)lacZ alpha fragment
cI 434BBa_C0052 Version 1 (Component)cI repressor from phage 434 (+LVA)
BBa_E0044BBa_E0044 Version 1 (Component)green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)
LacILacI Version 1 (Component) BBa_K786033BBa_K786033 Version 1 (Component)cascade proteins, which consists of casA, casB, casC, casD, casE
Las CIOBBa_I14016 Version 1 (Component)P(Las) CIO
lasrBBa_C0079 Version 1 (Component)lasR activator from P. aeruginosa PAO1(+LVA)
BBa_K1218019BBa_K1218019 Version 1 (Component)CASCADE Complex
BBa_K1559002BBa_K1559002 Version 1 (Component)rearranged CRISPR/Cas9 system without promoter
//cdscds Version 1 (Collection) amilCPBBa_K592009 Version 1 (Component)amilCP, blue chromoprotein
araCBBa_C0080 Version 1 (Component)araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
c2 P22BBa_C0053 Version 1 (Component)c2 repressor from Salmonella phage P22 (+LVA)
Cse1BBa_K1218003 Version 1 (Component)CRISPR CasA E. coli (Modern)
BBa_M1013BBa_M1013 Version 1 (Component)Complete Construct for Type I Phasin Secretion
OriTFBBa_J01002 Version 1 (Component)OriT-F (Origin of Transfer for the F-plasmid nic region)