BBa_K1677391
BBa_K1677391 Version 1 (Component)
IBV Pseudoknot

BBa_K1677800
BBa_K1677800 Version 1 (Component)
IPTG inducible promoter with IBV pseudoknot

BBa_K1677743
BBa_K1677743 Version 1 (Component)
TGV Pseudoknot

BBa_K1677369
BBa_K1677369 Version 1 (Component)
MMTV Pseudoknot

BBa_K1210001
BBa_K1210001 Version 1 (Component)
PK401 Pseudoknot

BBa_K1677666
BBa_K1677666 Version 1 (Component)
HCV229E Pseudoknot

BBa_K1677802
BBa_K1677802 Version 1 (Component)
IPTG inducible promoter with MMTV pseudoknot

BBa_K1677801
BBa_K1677801 Version 1 (Component)
IPTG inducible promoter with TGV pseudoknot

BBa_J5532
BBa_J5532 Version 1 (Component)
inv

BBa_J72130
BBa_J72130 Version 1 (Component)
{Pflu-inv}

BBa_K079012
BBa_K079012 Version 1 (Component)
mbv

pCpcG2 inv
BBa_K566009 Version 1 (Component)
pCpcG2 promoter, inverted sequence (Green light inducible)

BBa_K1431403
BBa_K1431403 Version 1 (Component)
gRNA2 for HBV

BBa_K1431402
BBa_K1431402 Version 1 (Component)
gRNA1 for HBV

BBa_K1180000
BBa_K1180000 Version 1 (Component)
anti-HBV siRNA

PenI inv
BBa_K566010 Version 1 (Component)
PenI repressor optimized for E. coli (inverted sequence)

OL Inv
BBa_K566035 Version 1 (Component)
OL region from Lambda (Inverted)

cI Inv
BBa_K566038 Version 1 (Component)
cI repressor from Lambda phage optimized for E. coli cI +LVA (Inverted)

BBa_K1431413
BBa_K1431413 Version 1 (Component)
target sequence2 for HBV

BBa_J331002
BBa_J331002 Version 1 (Component)
Dipeptidyl Peptidase IV

BBa_K624062
BBa_K624062 Version 1 (Component)
pYMB essentials + RBS(trunc.) + Inv

BBa_K624058
BBa_K624058 Version 1 (Component)
pYMB essentials + RBS(Pmsp3) + Inv

BBa_J72217
BBa_J72217 Version 1 (Component)
MC1061 ΔdapD O16::PglpT-rbs2.inv

BBa_J72216
BBa_J72216 Version 1 (Component)
MC1061 ΔdapD O16::PglpT-rbs1.inv

BBa_K896012
BBa_K896012 Version 1 (Component)
Inv+LLO+RFP ( reporter for invasin& listeriolysin)

RBS.3 Inv
BBa_K566037 Version 1 (Component)
RBS.3 (Inverted)

BBa_M13004
BBa_M13004 Version 1 (Component)
M13K07 gene IV

rhiI
BBa_K594005 Version 1 (Component)
a luxI homologous AHL synthetase from Rhzobium leguminosarum bv. vicae IMA57

BBa_K624048
BBa_K624048 Version 1 (Component)
RBS(trunc.) + LLO + RBS(trunc.) + Inv + RBS(trunc.) + ECFP

BBa_K624047
BBa_K624047 Version 1 (Component)
RBS(pmsp3) + LLO + RBS(pmsp3) + Inv + RBS(pmsp3) + ECFP

BBa_K1442113
BBa_K1442113 Version 1 (Component)
siRNA for DPP-IV

BBa_M13504
BBa_M13504 Version 1 (Component)
M13K07 gene IV RBS

BBa_M13104
BBa_M13104 Version 1 (Component)
M13K07 gene IV promoter

DPP-IV
BBa_K1442111 Version 1 (Component)
DPP-IV (our siRNA target)

XylE ib
BBa_K1062003 Version 1 (Component)
Guide RNA (gRNA) target for XylE (end of gene)

BBa_M45112
BBa_M45112 Version 1 (Component)
Protein Reduces Uranium (VI) to Uranium (IV)

BBa_K2033003
BBa_K2033003 Version 1 (Component)
Isovaleryl-HSL (IV-HSL) Receiver Device - BjaR

BBa_K1442026
BBa_K1442026 Version 1 (Component)
siRNA target to human DPP-IV mRNA

BBa_K1100151
BBa_K1100151 Version 1 (Component)
AAC(6')-Ib translational unit with B0015 terminator

BBa_M45134
BBa_M45134 Version 1 (Component)
Uranium reduction from VI valent state to IV valent state

BBa_K1431412
BBa_K1431412 Version 1 (Component)
target sequence1 for HBV

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.