BBa_K1362466
BBa_K1362466 Version 1 (Component)
Intein protease extein

Intein
BBa_K300004 Version 1 (Component)
Engineered pH-inducible intein (codon optimized for E. coli) - internal domain

BBa_K1362251
BBa_K1362251 Version 1 (Component)
Intein protease

BBa_K1362050
BBa_K1362050 Version 1 (Component)
Intein protease - T7RBS-His-SspDnaBC

Intein
BBa_K1483003 Version 1 (Component)
Ssp GyrB Split Intein

Intein
BBa_K1947002 Version 1 (Component)
Intein

NpuDnaE(N)
BBa_K1362400 Version 1 (Component)
NpuDnaE N-Intein cloning piece

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

NpuDnaE(C)
BBa_K1362401 Version 1 (Component)
NpuDnaE C-Intein cloning piece

CWE
BBa_K1362433 Version 1 (Component)
CWE (extein)

Intein C-t
BBa_K2075024 Version 1 (Component)
Intein and Intein Linker (C-term)

Intein N-t
BBa_K2075010 Version 1 (Component)
Intein and Intein linker (N-term)

His6
BBa_K1362431 Version 1 (Component)
N-terminal His6 (C-Intein)

BBa_I712015
BBa_I712015 Version 1 (Component)
HIV-1 protease cleavage site

BBa_K1362444
BBa_K1362444 Version 1 (Component)
GGG linker with RGK (extein)

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1362051
BBa_K1362051 Version 1 (Component)
Intein protease with arabinose inducible regulatory promoter/ repressor araC-Pbad-T7RBS-His-SspDnaBC

Intein
BBa_K2075002 Version 1 (Component)
Intein

BBa_K1362460
BBa_K1362460 Version 1 (Component)
RGK (extein)

Intein
BBa_K2075025 Version 1 (Component)
Intein N- and C-Term + Linker

BBa_K1491024
BBa_K1491024 Version 1 (Component)
ER Intein

BBa_K1638034
BBa_K1638034 Version 1 (Component)
Sce VMA Intein

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

BBa_K1491026
BBa_K1491026 Version 1 (Component)
Dead ER Intein

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SspDnaB(N)
BBa_K1362408 Version 1 (Component)
SspDnaB N-Intein cloning piece

CircNpu
BBa_K1362000 Version 1 (Component)
NpuDnaE intein RFC[105] circularization construct

BBa_K1903019
BBa_K1903019 Version 1 (Component)
Intein N SSp DnaB S1

SspDnaB(C)
BBa_K1362409 Version 1 (Component)
SspDnaB C-Intein cloning piece

1Gate-NpuD
BBa_K1362100 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

1GateNpuE
BBa_K1362101 Version 1 (Component)
RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)

Intron
BBa_J176014 Version 1 (Component)
Intron

BBa_J32009
BBa_J32009 Version 1 (Component)
4HT Dependent Intein

TerDnaE3(N
BBa_K1362404 Version 1 (Component)
TerDnaE3 N-Intein cloning piece

SspGyrB(N)
BBa_K1362406 Version 1 (Component)
SspGyrB N-Intein cloning piece

SspDnaX(N)
BBa_K1362402 Version 1 (Component)
SspDnaX N-Intein cloning piece

TerDnaE3(C
BBa_K1362405 Version 1 (Component)
TerDnaE3 C-Intein cloning piece

SspGyrB(C)
BBa_K1362407 Version 1 (Component)
SspGyrB C-Intein cloning piece

Gp41-1(N)
BBa_K1362412 Version 1 (Component)
Gp41-1 N-Intein cloning piece

SspDnaX(C)
BBa_K1362403 Version 1 (Component)
SspDnaX C-Intein cloning piece

NpuDnaE(C)
BBa_K1362479 Version 1 (Component)
nonsplicing NpuDnaE C-Intein cloning piece

BBa_K1465230
BBa_K1465230 Version 1 (Component)
T7-RBS Intein-tag

TerDnaE3(N
BBa_K1362130 Version 1 (Component)
RBS + TerDnaE3 N-intein RFC[105] assembly construct (with His6)

SspDnaB(N)
BBa_K1362140 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] assembly construct (with His6)

SspDnaE(N)
BBa_K1362410 Version 1 (Component)
SspDnaE N-Intein cloning piece

1NpuE(N*)
BBa_K1362102 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] non-splicing control construct (with His6)

TerDnaE3(C
BBa_K1362131 Version 1 (Component)
RBS + TerDnaE3 C-intein RFC[105] assembly construct (with His6)

SspDnaB(C)
BBa_K1362141 Version 1 (Component)
RBS + SspDnaB C-intein RFC[105] assembly construct (with His6)

SspDnaX(N)
BBa_K1362110 Version 1 (Component)
RBS + SspDnaX N-intein RFC[105] assembly construct (with His6)

SspGyrB(N)
BBa_K1362120 Version 1 (Component)
RBS + SspGyrB N-intein RFC[105] assembly construct (with His6)