CnU6
CnU6_terminator Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans JEC21

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

BBa_B0015
BBa_B0015 Version 1 (Component)
double terminator (B0010-B0012)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_B0011
BBa_B0011 Version 1 (Component)
LuxICDABEG (+/-)

BBa_B1001
BBa_B1001 Version 1 (Component)
Terminator (artifical, small, %T~=90)

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_K1051006
BBa_K1051006 Version 1 (Component)
Saccharomycescerevisiae TBY-1 terminator with stopcodon

BBa_K1051053
BBa_K1051053 Version 1 (Component)
K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 terminator)

BBa_K1051008
BBa_K1051008 Version 1 (Component)
S. cerevisiae CAA terminator with stopcodon

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

BBa_K1051000
BBa_K1051000 Version 1 (Component)
Stop codon free RFP in RFC[23] standard

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

BBa_B0014
BBa_B0014 Version 1 (Component)
double terminator (B0012-B0011)

RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z

BBa_B0025
BBa_B0025 Version 1 (Component)
double terminator (B0015), reversed

BBa_K1051055
BBa_K1051055 Version 1 (Component)
K1051001 (non stop codon ECFP + K1051008 (Stop codon + CAA terminator)

BBa_K1051361
BBa_K1051361 Version 1 (Component)
K1051301(clb2 promoter) + K1051002 (non stop codon EYFP) + K1051008 (Stop codon + CAA terminator)

BBa_K1051307
BBa_K1051307 Version 1 (Component)
k1051304(cln3 promoter)+k1051062(K1051004 (non stop codon morange) + K1051006 (Stop codon +TBY-1term

BBa_K1051356
BBa_K1051356 Version 1 (Component)
K1051301(clb2 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 term

BBa_K1051363
BBa_K1051363 Version 1 (Component)
K1051303 (clb6 promoter) + K1051002 (non stop codon EYFP) + K1051008 (Stop codon + CAA terminator)

BBa_K1051360
BBa_K1051360 Version 1 (Component)
K1051300(cln2 promoter) + K1051002 (non stop codon EYFP) + K1051008 (Stop codon + CAA terminator)

BBa_K1051364
BBa_K1051364 Version 1 (Component)
K1051304 (cln3 promoter) + K1051002 (non stop codon EYFP) + K1051008 (Stop codon + CAA terminator)

BBa_K1051362
BBa_K1051362 Version 1 (Component)
K1051302(clb5 promoter) + K1051002 (non stop codon EYFP) + K1051008 (Stop codon + CAA terminator)

BBa_K1051359
BBa_K1051359 Version 1 (Component)
K1051304(cln3 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 term

BBa_K1051355
BBa_K1051355 Version 1 (Component)
K1051300 (cln2 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 ter

BBa_K1051358
BBa_K1051358 Version 1 (Component)
K1051303(clb6 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 term

BBa_K1051357
BBa_K1051357 Version 1 (Component)
K1051302 (clb5 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 ter

BBa_K1051371
BBa_K1051371 Version 1 (Component)
K1051301 (clb2 promoter) + K1051004 (non stop codon mOrange) + K1051008 (Stop codon + CAA terminator

BBa_K1051058
BBa_K1051058 Version 1 (Component)
K1051002 (non stop codon EYFP + K1051008 (Stop codon +CCA terminator)

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

BBa_K1051352
BBa_K1051352 Version 1 (Component)
K1051302 (clb5 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)

BBa_K1051353
BBa_K1051353 Version 1 (Component)
K1051303 (clb6 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)

BBa_K1051372
BBa_K1051372 Version 1 (Component)
K1051302 (clb5 promoter) + K1051004 (non stop codon mOrange) + K1051008 (Stop codon + CAA terminator

BBa_K1051370
BBa_K1051370 Version 1 (Component)
K1051300 (cln2 promoter) + K1051004 (non stop codon mOrange) + K1051008 (Stop codon + CAA terminator

BBa_K1051350
BBa_K1051350 Version 1 (Component)
K1051300 (cln2 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)