ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
NLSBBa_J176008 Version 1 (Component)NLS
NLSmycSTOPBBa_J176031 Version 1 (Component)NLS myc stop
BBa_K2040122BBa_K2040122 Version 1 (Component)KillerRed + NLS
BBa_K1491015BBa_K1491015 Version 1 (Component)Codon Optimized KillerRed
NLS+TermBBa_K165018 Version 1 (Component)SV40 nuclear localization sequence + ADH1 transcription terminator
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
BBa_K1491016BBa_K1491016 Version 1 (Component)Lac Inducible Codon Optimized KillerRed
BBa_I712022BBa_I712022 Version 1 (Component)T7 RNA polymerase with NLS
BBa_I712069BBa_I712069 Version 1 (Component)RBS-T7 RNA polymerase with NLS
BBa_K2040120BBa_K2040120 Version 1 (Component)KillerRed + TtrpC
BBa_K929001BBa_K929001 Version 1 (Component)AID without NES, with NLS and Kozak sequence
BBa_K1641217BBa_K1641217 Version 1 (Component)killerred-B0015
BBa_K1495000BBa_K1495000 Version 1 (Component)KillerRed with Lactose Promoter
BBa_K1141005BBa_K1141005 Version 1 (Component)Red sensor-RBS-KillerRed
BBa_K2040121BBa_K2040121 Version 1 (Component)PgpdA + KillerRed + TtrpC
BBa_K1362057BBa_K1362057 Version 1 (Component)NLS as RFC[105] N-terminal insert
BBa_K2152006BBa_K2152006 Version 1 (Component)T7 promoter + RBS + killerred
BBa_K1914002BBa_K1914002 Version 1 (Component)E. coli Codon Optimised KillerRed(EOKR)
NLSBBa_M45417 Version 1 (Component)Nuclear Localization Sequence
BBa_K1141002BBa_K1141002 Version 1 (Component)Plac-RBS-KillerRed (IPTG-inducible)
BBa_K1923006BBa_K1923006 Version 1 (Component)NLS-FLAG-EGFP-dCas9-NLS encoding gene
BBa_K1641058BBa_K1641058 Version 1 (Component)pBAD-B0034-Killerred-B0015
BBa_J176125BBa_J176125 Version 1 (Component)NLS-pcDNA3.1+
BBa_K542010BBa_K542010 Version 1 (Component)Enhanced Lumazine Synthase (ELS)
BBa_J85801BBa_J85801 Version 1 (Component)rtTa3-2A-NLS-mKate2
BBa_K1733103BBa_K1733103 Version 1 (Component)Crisper/spCas9- with NLS
BBa_K1733099BBa_K1733099 Version 1 (Component)Crisper/spCas9- with NLS
BBa_K1923011BBa_K1923011 Version 1 (Component)Gal4BD-NLS-FLAG-EGFP-dCas9-NLS-Gal4AD encoding gene
BBa_K1471004BBa_K1471004 Version 1 (Component)NLS with PolyA.