Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
KBBa_J176012 Version 1 (Component)Kozak
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
His-StopBBa_K133133 Version 1 (Component)His affinity tag-stop
NLSmycSTOPBBa_J176031 Version 1 (Component)NLS myc stop
BBa_K844000BBa_K844000 Version 1 (Component)10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
RGD-His-StBBa_K133036 Version 1 (Component)RGD-His affinity tag-Stop
scarBBa_G0000 Version 1 (Component)SpeI/XbaI scar for RBS-CDS junctions
RFC105 ZBBa_K1362421 Version 1 (Component)C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z
Restriction_sitesRestriction_sites Version 1 (Component)SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI
BBa_J176171BBa_J176171 Version 1 (Component)Kozak-XbaI-NLS-6His-stop
NLSBBa_J176008 Version 1 (Component)NLS
BBa_K1051003BBa_K1051003 Version 1 (Component)Stop codon free GFP in RFC[23] standard
SX scarBBa_G0002 Version 1 (Component)SpeI/XbaI mixed site
BBa_K165002BBa_K165002 Version 1 (Component)Kozak sequence (yeast RBS)
BBa_K809601BBa_K809601 Version 1 (Component)GAL promoter (kozak) without start codon ATG
BBa_K1051000BBa_K1051000 Version 1 (Component)Stop codon free RFP in RFC[23] standard
STOPBBa_J176016 Version 1 (Component)STOP
BBa_K299107BBa_K299107 Version 1 (Component)T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)
StopBBa_K1362437 Version 1 (Component)Tripple A
NLS+TermBBa_K165018 Version 1 (Component)SV40 nuclear localization sequence + ADH1 transcription terminator
BBa_K929001BBa_K929001 Version 1 (Component)AID without NES, with NLS and Kozak sequence
HisTagBBa_J18909 Version 1 (Component)His tag (6xHis; E. coli - optimized)
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
T7stopBBa_J18913 Version 1 (Component)T7 terminator (incl. STOP)
BBa_K1875001BBa_K1875001 Version 1 (Component)This part contains a Kozak sequence
MFa1-KozakBBa_K792001 Version 1 (Component)Kozak sequence from yeast α-factor mating pheromone (MFα1)
BBa_K323083BBa_K323083 Version 1 (Component)His stop T7 terminator
BBa_M36117BBa_M36117 Version 1 (Component)Stop Codon
Kozak & stBBa_J63003 Version 1 (Component)designed yeast Kozak sequence
BBa_K1051004BBa_K1051004 Version 1 (Component)Stop codon free mOrange in RFC[23] standard
BBa_I712022BBa_I712022 Version 1 (Component)T7 RNA polymerase with NLS
BBa_K1051053BBa_K1051053 Version 1 (Component)K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 terminator)
TSSBBa_B0042 Version 1 (Component)Translational stop sequence