iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_J61101
BBa_J61101 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_J61120
BBa_J61120 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

BBa_C0261
BBa_C0261 Version 1 (Component)
AHL-making Enzyme, luxI (+RBS)

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

KpnI
BBa_I11071 Version 1 (Component)
KpnI restriction enzyme site with extra flanking nucleotides

Restriction_sites
Restriction_sites Version 1 (Component)
SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

BBa_J61100
BBa_J61100 Version 1 (Component)
Ribosome Binding Site Family Member

HindIII
BBa_I11076 Version 1 (Component)
HindIII restriction enzyme site with extra flanking nucleotides

SacII
BBa_I11078 Version 1 (Component)
SacII restriction enzyme site with extra flanking nucleotides

SalI
BBa_I11077 Version 1 (Component)
SalI restriction enzyme site with extra flanking nucleotides

BmtI
BBa_I11075 Version 1 (Component)
BmtI restriction enzyme site with extra flanking nucleotides

PvuI
BBa_I11073 Version 1 (Component)
PvuI restriction enzyme site with extra flanking nucleotides

XmaI
BBa_I11074 Version 1 (Component)
XmaI restriction enzyme site with extra flanking nucleotides

BBa_J85600
BBa_J85600 Version 1 (Component)
attB1 recombination site

ApaI
BBa_I11080 Version 1 (Component)
ApaI restriction site with extra flanking nucleotides

XbaI
BBa_I11082 Version 1 (Component)
XbaI restriction site with extra flanking nucleotides

BamHI
BBa_I11081 Version 1 (Component)
BamHI restriction site with extra flanking nucleotides

AclI
BBa_I11072 Version 1 (Component)
AclI restriction enzyme site with extra nucleotides

MluI
BBa_I11079 Version 1 (Component)
MluI restriction enzyme site with flanking nucleotides

mRFP sel.
BBa_K1362422 Version 1 (Component)
mRFP selection marker with outward BsaI restriction sites

<- BsaI
BBa_K1362423 Version 1 (Component)
BsaI reverse restriction site for RFC[105] cloning

BBa_I13457
BBa_I13457 Version 1 (Component)
Improved insertion site with two RNAse E cut sites

BBa_J64101
BBa_J64101 Version 1 (Component)
ColE1 biobrick plasmid with backward terminator

BamHI
BBa_K150008 Version 1 (Component)
BamHI restriction enzyme site

SpeI
BBa_M31992 Version 1 (Component)
SpeI restriction enzyme site

BglII
BBa_M31985 Version 1 (Component)
BglII restriction enzyme site

Acc65I
BBa_M31953 Version 1 (Component)
Acc65I restriction enzyme site

HpaI
BBa_M31950 Version 1 (Component)
HpaI restriction enzyme site

AatII
BBa_M31952 Version 1 (Component)
AatII restriction enzyme site

BBa_K1897031
BBa_K1897031 Version 1 (Component)
B0033 with flanking Scar sites