NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

lux pR
BBa_R0062 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pR)

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

lux pL
BBa_R0063 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pL)<br>

cI+luxR
BBa_R0065 Version 1 (Component)
Promoter (lambda cI and luxR regulated -- hybrid)

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

Prm +
BBa_I12007 Version 1 (Component)
Modified lambda Prm promoter (OR-3 obliterated)

BBa_C0261
BBa_C0261 Version 1 (Component)
AHL-making Enzyme, luxI (+RBS)

LuxR
BBa_I0462 Version 1 (Component)
luxR Protein Generator

BBa_J09855
BBa_J09855 Version 1 (Component)
Constitutive luxR device with pLuxR

BBa_J100042
BBa_J100042 Version 1 (Component)
LuxI with 3 clause 3-SAT problem

BBa_J100040
BBa_J100040 Version 1 (Component)
LuxI with 3 clause 2-SAT problem

Cat
BBa_I14033 Version 1 (Component)
P(Cat)

BBa_J100043
BBa_J100043 Version 1 (Component)
LuxI/GFP with 3 clause 3-SAT problem

BBa_K546000
BBa_K546000 Version 1 (Component)
Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.

BBa_J100041
BBa_J100041 Version 1 (Component)
LuxI/GFP with 3 clause 2-SAT problem

BBa_J100014
BBa_J100014 Version 1 (Component)
LuxI with 2 Clause 2-SAT Problem

BBa_J100044
BBa_J100044 Version 1 (Component)
LuxI with 4 clause 2-SAT problem

BBa_J100013
BBa_J100013 Version 1 (Component)
LuxI with 1 Clause 2-SAT Problem

HP3 3
BBa_B0101 Version 1 (Component)
HP3 3

BBa_J64101
BBa_J64101 Version 1 (Component)
ColE1 biobrick plasmid with backward terminator

BBa_J100045
BBa_J100045 Version 1 (Component)
LuxI/GFP with 4 clause 2-SAT problem

BBa_K309012
BBa_K309012 Version 1 (Component)
Unc-54 3' UTR (for use in C. elegans chassis)

BBa_J100000
BBa_J100000 Version 1 (Component)
Cre with 8bp restriction sites and 1-Clause 2-SAT Problem Inserted

BBa_J100016
BBa_J100016 Version 1 (Component)
2 Clause 2-SAT Problem with Frameshifted LuxI and a GFP Reporter

lux pR
BBa_R1062 Version 1 (Component)
Promoter, Standard (luxR and HSL regulated -- lux pR)<br>

BBa_K165011
BBa_K165011 Version 1 (Component)
Zif268-HIV binding sites (3)

RFC105 NN
BBa_K1362415 Version 1 (Component)
N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN

IR-3-GFP
BBa_K658017 Version 1 (Component)
LuxI->LuxR->lux pR-3->GFP

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

BBa_K1763424
BBa_K1763424 Version 1 (Component)
MaSp2-3(1C3)

BBa_K1061003
BBa_K1061003 Version 1 (Component)
RIP 3

BBa_M300052
BBa_M300052 Version 1 (Component)
m13k07 - Gene 3

BBa_J100015
BBa_J100015 Version 1 (Component)
1 Clause 2-SAT Problem with Frameshifted LuxI and a GFP Reporter

BBa_K658019
BBa_K658019 Version 1 (Component)
LuxI->LuxR->lux pR-3/5->GFP

BBa_K1351030
BBa_K1351030 Version 1 (Component)
LMU Bacillus RBS collection 3

lux pR-3
BBa_K658006 Version 1 (Component)
position 3 mutated promoter lux pR-3 (luxR & HSL regulated)

BBa_J202002
BBa_J202002 Version 1 (Component)
PBAD Mutant#3