Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

BBa_K1096001
BBa_K1096001 Version 1 (Component)
MazE protein (E. coli)

LuxR
BBa_I0462 Version 1 (Component)
luxR Protein Generator

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

tetA(C)f
BBa_J31007 Version 1 (Component)
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

BBa_K1096002
BBa_K1096002 Version 1 (Component)
MazF protein (E. coli)

BBa_K731722
BBa_K731722 Version 1 (Component)
T1 terminator from E. coli rrnB

BBa_J33201
BBa_J33201 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

BBa_I746200
BBa_I746200 Version 1 (Component)
FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.

BBa_J70029
BBa_J70029 Version 1 (Component)
RBS + mCherry gene, optimized for expression in Me. florum and E. coli

BBa_K2120008
BBa_K2120008 Version 1 (Component)
mazF

LacY
BBa_K079015 Version 1 (Component)
LacY transporter protein from E. coli

BBa_K912001
BBa_K912001 Version 1 (Component)
E. coli phytase (appa phytase)

sacB
BBa_K322921 Version 1 (Component)
B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.