Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

GFP
GFP Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S

HYG
Hygromicin_Resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

NEO (G418/Neomicin Resistance)
Neomicin_Resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

mCherry
mCherry Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

amdS
amdS Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

APP1
GenBank_CP047908_Region_1244167_1244926 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)

ACT1
GenBank_CP003820_Region_1242235_1243067 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

BBa_B0015
BBa_B0015 Version 1 (Component)
double terminator (B0010-B0012)

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_B0011
BBa_B0011 Version 1 (Component)
LuxICDABEG (+/-)

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_B0014
BBa_B0014 Version 1 (Component)
double terminator (B0012-B0011)

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

BBa_B0025
BBa_B0025 Version 1 (Component)
double terminator (B0015), reversed

BBa_B0016
BBa_B0016 Version 1 (Component)
Terminator (T7 RNAP specific, T_Phi)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_B0024
BBa_B0024 Version 1 (Component)
double terminator (B0012-B0011), reversed

BBa_B0017
BBa_B0017 Version 1 (Component)
double terminator (B0010-B0010)

BBa_B0035
BBa_B0035 Version 1 (Component)
RBS (B0030 derivative)

BBa_J61101
BBa_J61101 Version 1 (Component)
Ribosome Binding Site Family Member

spacer
BBa_B0040 Version 1 (Component)
Spacer.1 (generic)

BBa_J56012
BBa_J56012 Version 1 (Component)
Invertible sequence of dna includes Ptrc promoter

BBa_K1114107
BBa_K1114107 Version 1 (Component)
Bicistronic Design RBS 2, MoClo Format with BC fusion sites

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_B0053
BBa_B0053 Version 1 (Component)
Terminator (His)

BBa_J61120
BBa_J61120 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_B0013
BBa_B0013 Version 1 (Component)
TE from coliphage T7 (+/-)

pSC101
BBa_I50041 Version 1 (Component)
pSC101 origin of replication, reverse

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

BBa_B0029
BBa_B0029 Version 1 (Component)
1 bp deletion from B0031

BBa_K1114005
BBa_K1114005 Version 1 (Component)
The MoClo format of BBa_J23104 with AB fusion sites.

BBa_B0021
BBa_B0021 Version 1 (Component)
LuxICDABEG (+/-), reversed

BBa_K1114102
BBa_K1114102 Version 1 (Component)
MoClo format of a modified version of BBa_B0032 with BC fusion sites

BBa_K1114100
BBa_K1114100 Version 1 (Component)
MoClo format of a modified version of BBa_B0030 with BC fusion sites.

BBa_K1114101
BBa_K1114101 Version 1 (Component)
MoClo format of a modified version of BBa_B0031 with BC fusion sites.

BBa_B0062
BBa_B0062 Version 1 (Component)
Terminator (Reverse B0052)

BBa_B0054
BBa_B0054 Version 1 (Component)
Transcriptional terminator

TSS
BBa_B0042 Version 1 (Component)
Translational stop sequence

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP