mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

mCherry
BBa_J06505 Version 1 (Component)
monomeric RFP optimized for bacteria

BBa_K1769004
BBa_K1769004 Version 1 (Component)
Monomeric FYVE

BBa_K1769007
BBa_K1769007 Version 1 (Component)
Monomeric FYVE+GFP fusion protein

BBa_I712028
BBa_I712028 Version 1 (Component)
CherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence

BBa_K1351021
BBa_K1351021 Version 1 (Component)
Monomeric Red Fluorescent Protein from <i>Discosoma striata</i>

2 x FYVE
BBa_K1769000 Version 1 (Component)
Dimeric FYVE

mSA2
BBa_K1896000 Version 1 (Component)
Monomeric Streptavidin (mSA2)

BBa_K247000
BBa_K247000 Version 1 (Component)
monomeric RFP: Red Fluorescent Protein.

BBa_K1769001
BBa_K1769001 Version 1 (Component)
Dimeric FYVE + GFP

BBa_K1769006
BBa_K1769006 Version 1 (Component)
Dimeric FYVE (without stop codon)

BBa_K2170204
BBa_K2170204 Version 1 (Component)
Enhanced monomeric avidin (eMA) in RFC[25]

BBa_K823029
BBa_K823029 Version 1 (Component)
mKate2, a red monomeric fluorescent protein, B. subtilis optimized

BBa_K1351042
BBa_K1351042 Version 1 (Component)
Monomeric Red Fluorescent Protein from Discosoma striata

BBa_I769900
BBa_I769900 Version 1 (Component)
fibronectin monomer

BBa_I766231
BBa_I766231 Version 1 (Component)
Ser-Gly-Ser-Gly-Ser FYVE-EEA1 PH Domain

BBa_K823051
BBa_K823051 Version 1 (Component)
mKate2, a red monomeric fluorescent protein + RBS + Term

BBa_K2170001
BBa_K2170001 Version 1 (Component)
Secretory eukaryotic biotin binding receptor with enhanced monomeric avidin

BBa_K2170050
BBa_K2170050 Version 1 (Component)
Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin

BBa_I766337
BBa_I766337 Version 1 (Component)
FYVE-EEA1-GFP under medium constitutive promoter

BBa_K1361994
BBa_K1361994 Version 1 (Component)
Curli Fiber major monomer-CsgA with his tag

BBa_K1361999
BBa_K1361999 Version 1 (Component)
Curli Fiber major monomer-CsgA

BBa_K1384000
BBa_K1384000 Version 1 (Component)
silk masp2 monomer for ICA assembly

c1+c2+c3
BBa_K2027002 Version 1 (Component)
Long Bacterial Collagen Fiber Monomer with Cross-linking Domains

s1+s2+s3
BBa_K2027045 Version 1 (Component)
Long Bacterial Collagen Fiber Monomer without Cross-linking Domains

BBa_K758003
BBa_K758003 Version 1 (Component)
UAS, this part consists of five UAS sequences and hsp70 TATA.

BBa_K2066003
BBa_K2066003 Version 1 (Component)
Tet Monomer B w/ 8 bp spacer for ICA

BBa_K2066004
BBa_K2066004 Version 1 (Component)
Tet Monomer C w/ 8 bp spacer for ICA

BBa_K2066002
BBa_K2066002 Version 1 (Component)
Tet Monomer A w/ 8 bp spacer for ICA

BBa_K2066008
BBa_K2066008 Version 1 (Component)
Tet Monomer A w/ 64 bp spacer for ICA

BBa_K2066012
BBa_K2066012 Version 1 (Component)
Lac Monomer B w/ 16 bp spacer for ICA

BBa_K2066009
BBa_K2066009 Version 1 (Component)
Tet Monomer B w/ 64 bp spacer for ICA

BBa_K2066006
BBa_K2066006 Version 1 (Component)
Tet Monomer B w/ 16 bp spacer for ICA

BBa_K2066011
BBa_K2066011 Version 1 (Component)
Lac Monomer A w/ 16 bp spacer for ICA

BBa_K2066007
BBa_K2066007 Version 1 (Component)
Tet Monomer C w/ 16 bp spacer for ICA

BBa_K2066013
BBa_K2066013 Version 1 (Component)
Lac Monomer C w/ 16 bp spacer for ICA

BBa_K2066005
BBa_K2066005 Version 1 (Component)
Tet Monomer A w/ 16 bp spacer for ICA

BBa_K2066010
BBa_K2066010 Version 1 (Component)
Tet Monomer C w/ 64 bp spacer for ICA

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.