ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

iGEM to SBOL conversion
igem2sbol Version 1 (Activity)
Conversion of the iGEM parts registry to SBOL2.1

SyBiOntKB to SBOL conversion
SyBiOntKB2sbol Version 1 (Activity)
Conversion of the Synthetic Biology Ontology knowledge base to SBOL 2.1

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K1150010
BBa_K1150010 Version 1 (Component)
NLS

NLS
BBa_J176008 Version 1 (Component)
NLS

NLSmycSTOP
BBa_J176031 Version 1 (Component)
NLS myc stop

NLS+Term
BBa_K165018 Version 1 (Component)
SV40 nuclear localization sequence + ADH1 transcription terminator

GC Optimised Sequence Pull
GC_Optimised Version 1 (Activity)
Used to indicate that a sequence was GC optimised after pulling from the source and before submission

BBa_I712022
BBa_I712022 Version 1 (Component)
T7 RNA polymerase with NLS

BBa_I712069
BBa_I712069 Version 1 (Component)
RBS-T7 RNA polymerase with NLS

BBa_K929001
BBa_K929001 Version 1 (Component)
AID without NES, with NLS and Kozak sequence

BBa_P10401
BBa_P10401 Version 1 (Component)
Nos

BBa_K1362057
BBa_K1362057 Version 1 (Component)
NLS as RFC[105] N-terminal insert

BBa_J72121
BBa_J72121 Version 1 (Component)
{-NLS!}

BBa_K2040122
BBa_K2040122 Version 1 (Component)
KillerRed + NLS

BBa_K1547009
BBa_K1547009 Version 1 (Component)
Cas9 NLS

NLS
BBa_M45417 Version 1 (Component)
Nuclear Localization Sequence

BBa_K1923006
BBa_K1923006 Version 1 (Component)
NLS-FLAG-EGFP-dCas9-NLS encoding gene

BBa_K1680004
BBa_K1680004 Version 1 (Component)
SV40 NLS

BBa_K382035
BBa_K382035 Version 1 (Component)
LacI+NLS

BBa_J176125
BBa_J176125 Version 1 (Component)
NLS-pcDNA3.1+

BBa_K542010
BBa_K542010 Version 1 (Component)
Enhanced Lumazine Synthase (ELS)

BBa_J85801
BBa_J85801 Version 1 (Component)
rtTa3-2A-NLS-mKate2

BBa_K1733103
BBa_K1733103 Version 1 (Component)
Crisper/spCas9- with NLS

BBa_K1733099
BBa_K1733099 Version 1 (Component)
Crisper/spCas9- with NLS

BBa_K1923011
BBa_K1923011 Version 1 (Component)
Gal4BD-NLS-FLAG-EGFP-dCas9-NLS-Gal4AD encoding gene

BBa_K1471004
BBa_K1471004 Version 1 (Component)
NLS with PolyA.

BBa_K1547001
BBa_K1547001 Version 1 (Component)
PSAD GFP-NLS cassette

BBa_K105125
BBa_K105125 Version 1 (Component)
NLS + Adh1 terminator

BBa_K1150062
BBa_K1150062 Version 1 (Component)
NLS-BGH terminator

mRFP1-NLS
BBa_K678034 Version 1 (Component)
mRFP1-NLS, targeted to the nucleus

BBa_K426014
BBa_K426014 Version 1 (Component)
Nuclear Localization Signal (NLS)

BBa_I712055
BBa_I712055 Version 1 (Component)
myri - TEVP - mCherry - NLS

BBa_K1680021
BBa_K1680021 Version 1 (Component)
Dronpa caged Cre with NLS

BBa_K1680023
BBa_K1680023 Version 1 (Component)
Dronpa caged Cre with NLS

BBa_K1680024
BBa_K1680024 Version 1 (Component)
Dronpa caged Cre with NLS

BBa_K1680022
BBa_K1680022 Version 1 (Component)
Dronpa caged Cre with NLS

BBa_K782007
BBa_K782007 Version 1 (Component)
NicTAL12:NLS DNA binding domain

BBa_K1777005
BBa_K1777005 Version 1 (Component)
flexible linker with SV40 NLS