ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
NLSmycSTOPBBa_J176031 Version 1 (Component)NLS myc stop
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
RFC105 ZBBa_K1362421 Version 1 (Component)C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z
NLSBBa_J176008 Version 1 (Component)NLS
His-StopBBa_K133133 Version 1 (Component)His affinity tag-stop
BBa_K844000BBa_K844000 Version 1 (Component)10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
BBa_K1051003BBa_K1051003 Version 1 (Component)Stop codon free GFP in RFC[23] standard
RGD-His-StBBa_K133036 Version 1 (Component)RGD-His affinity tag-Stop
BBa_K1051000BBa_K1051000 Version 1 (Component)Stop codon free RFP in RFC[23] standard
STOPBBa_J176016 Version 1 (Component)STOP
StopBBa_K1362437 Version 1 (Component)Tripple A
NLS+TermBBa_K165018 Version 1 (Component)SV40 nuclear localization sequence + ADH1 transcription terminator
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
T7stopBBa_J18913 Version 1 (Component)T7 terminator (incl. STOP)
BBa_M36117BBa_M36117 Version 1 (Component)Stop Codon
BBa_K1051004BBa_K1051004 Version 1 (Component)Stop codon free mOrange in RFC[23] standard
BBa_J176171BBa_J176171 Version 1 (Component)Kozak-XbaI-NLS-6His-stop
c-MycBBa_I712007 Version 1 (Component)c-Myc affinity tag
BBa_I712022BBa_I712022 Version 1 (Component)T7 RNA polymerase with NLS
BBa_K1051053BBa_K1051053 Version 1 (Component)K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 terminator)
TSSBBa_B0042 Version 1 (Component)Translational stop sequence
BBa_I712069BBa_I712069 Version 1 (Component)RBS-T7 RNA polymerase with NLS
BBa_K1051009BBa_K1051009 Version 1 (Component)e.coli terminator ilvGEDA_T with stop codon
BBa_K1051002BBa_K1051002 Version 1 (Component)Stop codon free EYFP in RFC[23] standard
BBa_K1051001BBa_K1051001 Version 1 (Component)Stop codon free ECFP in RFC[23] standard
BBa_K929001BBa_K929001 Version 1 (Component)AID without NES, with NLS and Kozak sequence
BBa_I766217BBa_I766217 Version 1 (Component)Stop-D-BamHI-Adh Terminator Adaptor
dCas 9BBa_K1051800 Version 1 (Component)protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.
BBa_K112503BBa_K112503 Version 1 (Component)< myc ! in BBb
BBa_K1051502BBa_K1051502 Version 1 (Component)Sic1 mutation, non stop codon
BBa_K323083BBa_K323083 Version 1 (Component)His stop T7 terminator
BBa_K1362057BBa_K1362057 Version 1 (Component)NLS as RFC[105] N-terminal insert
BBa_K323130BBa_K323130 Version 1 (Component)stop T7 terminator