ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
CD4 signalBBa_I712009 Version 1 (Component)CD4 signal peptide; localizes to plasma membrane
NLS+TermBBa_K165018 Version 1 (Component)SV40 nuclear localization sequence + ADH1 transcription terminator
NLSBBa_J176008 Version 1 (Component)NLS
BBa_K426014BBa_K426014 Version 1 (Component)Nuclear Localization Signal (NLS)
BBa_I712028BBa_I712028 Version 1 (Component)CherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence
BBa_K209402BBa_K209402 Version 1 (Component)AarI A-B part, signal sequence-FLAG-YFP [SSFYFP]
NLSmycSTOPBBa_J176031 Version 1 (Component)NLS myc stop
BBa_K801030BBa_K801030 Version 1 (Component)Polypeptide containing SV40 nuclear localization sequence (SV40 NLS) for nuclear translocation
NLSBBa_M45417 Version 1 (Component)Nuclear Localization Sequence
BBa_M45416BBa_M45416 Version 1 (Component)Nuclear Localization Sequence
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
BBa_K1484104BBa_K1484104 Version 1 (Component)N7 nuclear localisation tag
BBa_J36835BBa_J36835 Version 1 (Component)Lipoprotein signal peptide
BBa_K902019BBa_K902019 Version 1 (Component)S7 micrococcal nuclease
SPzim17BBa_K809201 Version 1 (Component)signal peptide of ZIM17
mRFP1-NLSBBa_K678034 Version 1 (Component)mRFP1-NLS, targeted to the nucleus
BBa_I712022BBa_I712022 Version 1 (Component)T7 RNA polymerase with NLS
BBa_K1729002BBa_K1729002 Version 1 (Component)Yeast MRPS12 mitochondrial localization signal
BBa_K1431201BBa_K1431201 Version 1 (Component)NLS, lead protein into the nucleus
BBa_K1659301BBa_K1659301 Version 1 (Component)Micrococcal Nuclease (DNase) fused at N-terminal with DsbA signal sequence
BBa_I712069BBa_I712069 Version 1 (Component)RBS-T7 RNA polymerase with NLS
BBa_K554002BBa_K554002 Version 1 (Component)HlyA secretion signal peptide
BBa_K523021BBa_K523021 Version 1 (Component)pVIII periplasmic localisation signal (for BioSandwich)
SV40 NLSBBa_J63008 Version 1 (Component)SV40 nuclear localization sequence from SV40; yeast codon optimized
BBa_K929001BBa_K929001 Version 1 (Component)AID without NES, with NLS and Kozak sequence
BBa_K496004BBa_K496004 Version 1 (Component)Reporter of Salmonella secretion signal with NLS, GFP, etc
BBa_K215001BBa_K215001 Version 1 (Component)Secretion Signal and Streptavidin Binding Tags
BBa_K1362057BBa_K1362057 Version 1 (Component)NLS as RFC[105] N-terminal insert
BBa_K1159109BBa_K1159109 Version 1 (Component)Micrococcal nuclease (Thermonuclease) with N-terminal SV40 NLS in RFC[25]
BBa_K2040122BBa_K2040122 Version 1 (Component)KillerRed + NLS
BBa_K1510009BBa_K1510009 Version 1 (Component)E.coli Signal sequence YebF