TRP1
GenBank_CP003828_Region_1023919_1024166 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubi H99

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

iGEM to SBOL conversion
igem2sbol Version 1 (Activity)
Conversion of the iGEM parts registry to SBOL2.1

SyBiOntKB to SBOL conversion
SyBiOntKB2sbol Version 1 (Activity)
Conversion of the Synthetic Biology Ontology knowledge base to SBOL 2.1

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_J119126
BBa_J119126 Version 1 (Component)
P5-Bd24 ligated to trpR (S05028+J119124)

Scar 25
BBa_B0105 Version 1 (Component)
RFC 25 Scar Sequence

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K864400
BBa_K864400 Version 1 (Component)
Ptac, trp & lac regulated promoter

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_J119024
BBa_J119024 Version 1 (Component)
BD18 bicistronic translational junction - Medium effiency

p15A
BBa_I50032 Version 1 (Component)
p15A replication origin

BBa_J100134
BBa_J100134 Version 1 (Component)
P5+BD24+gst9

BBa_J100133
BBa_J100133 Version 1 (Component)
P5+BD24+gst1

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

BBa_S05017
BBa_S05017 Version 1 (Component)
J119029:J119024

CD4 signal
BBa_I712009 Version 1 (Component)
CD4 signal peptide; localizes to plasma membrane

BBa_J100254
BBa_J100254 Version 1 (Component)
Part J119137 + P5

RFC 37
BBa_K245122 Version 1 (Component)
P5

RFC 37
BBa_K245009 Version 1 (Component)
RFC 37 Scar Sequence

BBa_S05022
BBa_S05022 Version 1 (Component)
J119029:J119027

BBa_S05007
BBa_S05007 Version 1 (Component)
J119029:J119025

BBa_S05012
BBa_S05012 Version 1 (Component)
J119029:J119026

BBa_S05027
BBa_S05027 Version 1 (Component)
J119029:J119028

BBa_S01022
BBa_S01022 Version 1 (Component)
B0034.E0020

BBa_J119125
BBa_J119125 Version 1 (Component)
Ptrp+(rbs-mRFP) (J119123 ligated to S04419)

BBa_S05048
BBa_S05048 Version 1 (Component)
B0034:K748000

BBa_J119031
BBa_J119031 Version 1 (Component)
P5 Promoter - Highest efficiency

BBa_J119028
BBa_J119028 Version 1 (Component)
BD24 bicistronic translational junction - Highest efficiency

GC Optimised Sequence Pull
GC_Optimised Version 1 (Activity)
Used to indicate that a sequence was GC optimised after pulling from the source and before submission

BBa_J119401
BBa_J119401 Version 1 (Component)
tCloneTet+PDB riboswitch with P5 and BD2

BBa_J119123
BBa_J119123 Version 1 (Component)
Promoter-tryptophan, Ptrp, repressible promoter of the tryptophan operon

TrpR
BBa_K191007 Version 1 (Component)
Trp promoter

BBa_J119127
BBa_J119127 Version 1 (Component)
For Testing New Promoters via Golden Gate Assembly with BsmBI

BBa_P10598
BBa_P10598 Version 1 (Component)
GoldenBraid destination vector omega insert

BBa_P10599
BBa_P10599 Version 1 (Component)
GoldenBraid destination vector alpha insert

BBa_S01008
BBa_S01008 Version 1 (Component)
B0034.C0070.E0420

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

P5
BBa_K100003 Version 1 (Component)
Edited Xylose Regulated Bi-Directional Operator 3