Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

pSB1C3
pSB1C3 Version 1 (Component)
High copy BioBrick assembly plasmid

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

Prm +
BBa_I12007 Version 1 (Component)
Modified lambda Prm promoter (OR-3 obliterated)

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

BBa_J119142
BBa_J119142 Version 1 (Component)
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

BBa_J119300
BBa_J119300 Version 1 (Component)
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

RE
BBa_J3101 Version 1 (Component)
Recombinational Enhancer (RE) for Hin/Hix inverting

rhII
BBa_C0070 Version 1 (Component)
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL

BBa_I13401
BBa_I13401 Version 1 (Component)
GFP reporter for RHS of library test constructs

BBa_J23008
BBa_J23008 Version 1 (Component)
[key3c] riboregulator for lock3 variants

BBa_K2116090
BBa_K2116090 Version 1 (Component)
Spacer for BB assembly

scar
BBa_G0000 Version 1 (Component)
SpeI/XbaI scar for RBS-CDS junctions

BBa_K880005
BBa_K880005 Version 1 (Component)
Strong promoter, strong RBS combination for high expression levels of proteins

Prm +
BBa_I12006 Version 1 (Component)
Modified lamdba Prm promoter (repressed by 434 cI)

lox
BBa_J61046 Version 1 (Component)
[Lox] site for recombination

BBa_K1159993
BBa_K1159993 Version 1 (Component)
25/25 Assembly Scar

BsaI ->
BBa_K1362425 Version 1 (Component)
BsaI restriction site for RFC[???] cloning (in part in prefix)

<- BsaI
BBa_K1362423 Version 1 (Component)
BsaI reverse restriction site for RFC[105] cloning

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

mCherry
BBa_J06505 Version 1 (Component)
monomeric RFP optimized for bacteria

BBa_K209402
BBa_K209402 Version 1 (Component)
AarI A-B part, signal sequence-FLAG-YFP [SSFYFP]

BBa_K797013
BBa_K797013 Version 1 (Component)
Plasmid Backbone for golden gate assembly

cheZ
BBa_K629003 Version 1 (Component)
cheZ, chemotaxis regulator, protein phosphatase for CheY

BBa_J100028
BBa_J100028 Version 1 (Component)
placeholder insert for BsaI Golden Gate Assembly of promoter

BBa_J119141
BBa_J119141 Version 1 (Component)
Device for Testing New Promoters via BsgI Golden Gate Assembly

pSB1AT3
pSB1AT3 Version 1 (Component)
High copy BioBrick assembly plasmid

Pveg-spoVG
BBa_K143053 Version 1 (Component)
Promoter Pveg and RBS spoVG for B. subtilis

OriTR
BBa_J01003 Version 1 (Component)
OriT-R (Origin of transfer for the R-plasmid nic region)

lasI
BBa_C0178 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)

BBa_K648029
BBa_K648029 Version 1 (Component)
OR, operating region for Lambda switch

BBa_K209399
BBa_K209399 Version 1 (Component)
AarI A-D acceptor vector

BBa_K560013
BBa_K560013 Version 1 (Component)
BsaI Golden Gate Assembly No.2

BBa_K560014
BBa_K560014 Version 1 (Component)
BsaI Golden Gate Assembly No.3

BBa_K560012
BBa_K560012 Version 1 (Component)
BsaI Golden Gate Assembly No.1

BBa_K560015
BBa_K560015 Version 1 (Component)
BsaI Golden Gate Assembly No.4

BBa_J119127
BBa_J119127 Version 1 (Component)
For Testing New Promoters via Golden Gate Assembly with BsmBI

BBa_J23009
BBa_J23009 Version 1 (Component)
[key3d] riboregulator for lock3 variants

BBa_J70042
BBa_J70042 Version 1 (Component)
6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)

Pveg
BBa_K143012 Version 1 (Component)
Promoter veg a constitutive promoter for B. subtilis

yfp
BBa_K165005 Version 1 (Component)
Venus YFP, yeast optimized for fusion

BBa_J119139
BBa_J119139 Version 1 (Component)
Device for Testing New Promoters via Golden Gate Assembly

BBa_J100104
BBa_J100104 Version 1 (Component)
For Testing New Promoters via Golden Gate Assembly v2

BBa_J119138
BBa_J119138 Version 1 (Component)
Device for Testing New Promoters via Golden Gate Assembly